Composition for reducing skin purpurin and preparation method and application thereof
Technical Field
The invention belongs to the field of cosmetics, and particularly relates to a composition for reducing skin purpurin, and a preparation method and application thereof.
Background
The severe manifestations of purpuria mainly include acne, pimple, nodule, cyst, sunken skin, hyperplastic paralysis, etc., especially the skin damage on face brings heavy burden to the spirit and mind of the patient, which may cause the patient to have bad mental states such as anxiety, depression, etc., and affect the life, study and work of the patient. The face is usually the first thing a person notices, and purpura can negatively affect the perception of others. The skin purpura is a chronic inflammatory disease of the pilosebaceous glands with high incidence rate, and the disease can affect people of any age group. The pathogenesis of the skin purpurin is complex and is the result of multifactorial action, the excessive secretion of sebaceous glands mediated by initial hormone, the abnormal keratinization and inflammatory reaction of the opening of hair follicle sebaceous glands in the later period, the colonization of propionibacterium acnes and the delayed immune reaction are involved, and external factors and genetic factors are involved in the generation. Purpurin is a metabolite of bacteria, and if the skin contains much purpurin, pores are easily blocked, and skin problems such as skin blockage and the like are easily caused.
The most interesting item of the oil skin, purpurin, is that we often see the skin in different parts, and the observation with the skin image analysis system VISIA reveals that especially the T-region of the face (forehead and nose) emits a lot of fluorescence, which is actually the metabolite of bacteria parasitizing in the mouth of the hair follicle, also called "porphyrin". Porphyrin can present fluorescent points with different colors under the excitation of ultraviolet light, for example, brick red bright spots are related to propionibacterium, blue white fluorescence is related to malassezia folliculitis, and the extinguishment of the porphyrin points is of great significance for controlling skin inflammation and controlling oil.
CN106511153A discloses a nano composition with acne removing efficacy and a cosmetic or skin care product containing the same. The nano composition with the acne removing effect comprises the following components in percentage by weight: 0.1-5% of purslane extract, 0.5-5% of compound consisting of keratin amino acids, betaine and chicory root extract, 0.78-0.5% of quaternary ammonium salt-730.001, 1-10% of emulsifier, 5-15% of grease and the balance of water. The nano composition with the acne removing effect and the cosmetic or skin care product containing the composition can inhibit the secretion of skin sebaceous gland grease, so that the skin sebaceous gland grease is reduced, but the skin purpura is still poor in effect of radically treating the skin purpura, and is easy to repeat after long-term use.
CN110227058A discloses a pleiotrophin composition comprising: 0.01-1% of a hypoxic pretreated pleiotropic factor solution, 0.05-1% of sodium hyaluronate, 2-10% of mannitol, 0.5-2% of sodium ascorbate, 0.5-2% of panthenol, 0.05-0.2% of phenoxyethanol, and the balance of water. The pleiotropic factor composition provided by the invention can be used for nourishing and filling tissues of skin, and the subcutaneous deep tissue participates in the repair of basal cells, but the pleiotropic factor composition has poor effect on radically treating skin purpura and is easy to repeat after long-term use.
Therefore, the development of a cosmetic capable of fundamentally reducing skin purpurin is the focus of current research in the art.
Disclosure of Invention
Aiming at the defects of the prior art, the invention aims to provide a composition for reducing skin purpurin and a preparation method and application thereof. The composition can effectively inhibit inflammation caused by bacteria and inhibit the growth of pathogenic flora, thereby reducing skin purpurin, and has the function of inhibiting oil secretion, effectively preventing the hyperkeratosis of hair follicle sebaceous gland ducts, and good oil control effect.
In order to achieve the purpose, the invention adopts the following technical scheme:
in a first aspect, the present invention provides a skin purple reducing composition comprising a yeast/rice fermentation filtrate, a yeast/zinc fermentation, a yeast dehiscence yeast fermentation lysate and a hamamelis virginiana extract.
In the invention, the saccharomycete/rice fermentation product filtrate, the saccharomycete/zinc fermentation product, the schizosaccharomyces cerevisiae fermentation product lysate and the hamamelis virginiana extract are matched with each other for synergistic interaction, so that the effects of cleaning and controlling oil and repairing barriers on facial skin are effectively improved, the secretion of oil is inhibited, and the balance of skin microecology is adjusted; regulating pH value of skin microenvironment, inhibiting growth of pathogenic bacteria, effectively inhibiting oil secretion, and inhibiting hyperkeratosis of pilosebaceous canal, thereby reducing skin purpurin.
In the invention, the yeast/rice fermentation product filtrate is prepared by selecting excellent rice and naturally fermenting the rice by natural yeast, and is rich in various active ingredients required by skin, such as oligosaccharide, organic acid, polypeptide, amino acid and the like. The probiotic bacteria can be simply and quickly absorbed and utilized by the metabolic activity of common skin probiotics (such as lactobacillus pentosus, micrococcus crusus and the like) and not absorbed and utilized by common skin pathogenic bacteria (such as propionibacterium acnes and staphylococcus aureus), so that the healthy growth of the probiotic bacteria in the skin is facilitated, the growth and the propagation of the pathogenic bacteria are competitively inhibited, and a good probiotic bacteria ecological environment beneficial to the skin is established; meanwhile, the skin care product has the effects of quickly activating skin cells, inhibiting skin inflammation and strengthening a skin cutin protective barrier, and achieves the purpose of fundamentally reducing skin purpurin.
In the invention, the yeast/zinc fermentation product refers to yeast essence substance extracted from zinc-containing yeast thalli after biological enzymolysis, contains more than 4500ppm chelated organic zinc besides a large amount of amino acid, peptide, nucleic acid and other bioactive substances necessary for skin, and has good anti-inflammation and acne-removing functions because of the chelated form of zinc and amino acid, and the stimulation of single zinc to skin is reduced.
In the present invention, the yeast fermentation lysate of the binary fission can regulate the gene expression and metabolism of cells through signal pathways of the cells, thereby exerting an effect of improving the skin condition. The lysate of the yeast fermentation product of the second split can generate small molecules which comprise vitamin B group, minerals, amino acid and the like and are beneficial to skin care, can strengthen the metabolism of stratum corneum and effectively inhibit the secretion of grease.
In the invention, the hamamelis virginiana extract contains active tannin components, and can help skin to be astringent, promote the skin to absorb nutrient substances of yeast/rice fermentation product filtrate, yeast/zinc fermentation product and schizosaccharomyces cerevisiae fermentation product lysate, and further improve the anti-inflammatory and soothing functions of the facial skin. Meanwhile, the witch hazel has a soft and sensitive factor, so that the witch hazel can calm the skin, keep the balance of skin metabolism, eliminate discomfort of the skin and play a great role in the health of the skin in the contact process of the witch hazel and the skin.
Preferably, the composition comprises, in parts by weight: 0.05-10 parts of yeast/rice fermentation product filtrate, 0.05-10 parts of yeast/zinc fermentation product, 0.05-10 parts of schizosaccharomyces cerevisiae fermentation product lysate and 0.05-10 parts of hamamelis virginiana extract.
In the present invention, the content of the yeast/rice fermentation product filtrate is 0.05 to 10 parts, for example, 0.05 part, 0.1 part, 0.5 part, 1 part, 1.5 part, 2 parts, 2.5 parts, 3 parts, 3.5 parts, 4 parts, 4.5 parts, 5 parts, 5.5 parts, 6 parts, 6.5 parts, 7 parts, 7.5 parts, 8 parts, 8.5 parts, 9 parts, 9.5 parts, 10 parts.
In the present invention, the content of the yeast/zinc fermentation product is 0.05 to 10 parts, for example, 0.05 part, 0.1 part, 0.5 part, 1 part, 1.5 part, 2 parts, 2.5 parts, 3 parts, 3.5 parts, 4 parts, 4.5 parts, 5 parts, 5.5 parts, 6 parts, 6.5 parts, 7 parts, 7.5 parts, 8 parts, 8.5 parts, 9 parts, 9.5 parts, 10 parts.
In the present invention, the content of the secondary fission yeast fermentation product lysate may be, for example, 0.05 part, 0.1 part, 0.5 part, 1 part, 1.5 parts, 2 parts, 2.5 parts, 3 parts, 3.5 parts, 4 parts, 4.5 parts, 5 parts, 5.5 parts, 6 parts, 6.5 parts, 7 parts, 7.5 parts, 8 parts, 8.5 parts, 9 parts, 9.5 parts, or 10 parts.
In the present invention, the content of the witch hazel extract may be, for example, 0.05 parts, 0.1 parts, 0.5 parts, 1 part, 1.5 parts, 2 parts, 2.5 parts, 3 parts, 3.5 parts, 4 parts, 4.5 parts, 5 parts, 5.5 parts, 6 parts, 6.5 parts, 7 parts, 7.5 parts, 8 parts, 8.5 parts, 9 parts, 9.5 parts, 10 parts.
Preferably, the composition comprises, in parts by weight: 0.1-5 parts of yeast/rice fermentation product filtrate, 0.1-5 parts of yeast/zinc fermentation product, 0.1-5 parts of schizosaccharomyces cerevisiae fermentation product lysate and 0.1-5 parts of hamamelis virginiana extract.
In a second aspect, the present invention provides a method of preparing a skin purple reducing composition according to the first aspect, the method comprising the steps of: mixing yeast/rice fermentation product filtrate, yeast/zinc fermentation product, yeast schizosaccharomyces fermentation product lysate and hamamelis virginiana extract, stirring, and filtering to obtain the composition for reducing skin purpurin.
Preferably, the mixing and stirring temperature is 35-45 ℃, for example, can be 35 degrees, 36 degrees, 37 degrees, 38 degrees, 39 degrees, 40 degrees, 41 degrees, 42 degrees, 43 degrees, 44 degrees, 45 degrees.
Preferably, the rotation speed of the mixing and stirring is 200-400r/min, such as 200r/min, 220r/min, 240r/min, 260r/min, 280r/min, 300r/min, 320r/min, 340r/min, 360r/min, 380r/min, 400 r/min.
Preferably, the mixing and stirring time is 10-30min, such as 10min, 12min, 14min, 16min, 18min, 20min, 22min, 24min, 26min, 28min, 30 min.
Preferably, the filtration is microfiltration.
Preferably, the pore size of the micropores is 400-600 meshes, for example, 400 meshes, 420 meshes, 440 meshes, 460 meshes, 480 meshes, 500 meshes, 520 meshes, 540 meshes, 560 meshes, 580 meshes, 600 meshes may be provided.
Preferably, the preparation method comprises the following steps: mixing and stirring the yeast/rice fermentation product filtrate, the yeast/zinc fermentation product, the schizosaccharomyces cerevisiae fermentation product lysate and the hamamelis virginiana extract at 35-45 ℃ at the rotating speed of 200-400r/min for 10-30min, and filtering through micropores with 400-600 meshes to obtain the composition for reducing the skin purpurin.
In a third aspect, the present invention provides the use of a skin purple reducing composition according to the first aspect in the preparation of a cosmetic.
In a fourth aspect, the present invention provides a cosmetic comprising a skin purple reducing composition according to the first aspect.
Preferably, the skin purple reducing composition is added in an amount of 0.05-20% by weight, for example, 0.05%, 0.1%, 0.5%, 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 12%, 14%, 16%, 18%, 20%, preferably 0.05-10% by weight of the total cosmetic weight;
preferably, the cosmetic comprises a mask, a lotion, a skin cream, a smoothing toner, a serum, a moisturizing cream or a lotion.
Compared with the prior art, the invention has the following beneficial effects:
according to the invention, the saccharomycetes/rice fermentation product filtrate, the saccharomycetes/zinc fermentation product, the schizosaccharomyces cerevisiae fermentation product lysate and the hamamelis virginiana extract are matched with each other, so that the synergistic effect is achieved, the effects of cleaning and controlling oil and repairing barriers on facial skin are improved, the secretion of oil is effectively inhibited, and the balance of skin microecology is adjusted; regulating pH value of skin microenvironment, inhibiting growth of pathogenic bacteria, effectively inhibiting oil secretion, and inhibiting hyperkeratosis of pilosebaceous canal, thereby reducing skin purpurin.
Drawings
FIG. 1 is a diagram of the purple characteristic of the face of a subject before trial.
Fig. 2 is a facial purple profile of a subject 1 week after using the skin purple reducing composition prepared in example 1.
Fig. 3 is a facial purple profile of a subject 2 weeks after using the skin purple reducing composition prepared in example 1.
Detailed Description
The technical solution of the present invention is further explained by the following embodiments. It should be understood by those skilled in the art that the examples are only for the understanding of the present invention and should not be construed as the specific limitations of the present invention.
In the following examples, the yeast/rice fermentation product filtrate was prepared as follows:
(1) preparing materials: cleaning and drying rice, and crushing the rice to 100 meshes; preparing yeast fermentation bacteria liquid with the concentration of 110 cfu/mL; preparing a fermentation medium: based on 100 percent of the total mass of the fermentation medium, 1 percent of peptone and 4 percent of glucose, and the balance of distilled water;
(2) fermentation: mixing the above bacteria liquid, rice and fermentation medium, adjusting pH to 6, and fermenting in a shaking table at 30 deg.C and 100r/min for 40 hr to obtain fermentation liquid;
(3) centrifuging: centrifuging the fermentation liquor for 30min under the conditions of 5000r/min and the centrifugal radius of 10cm, removing the precipitate, and collecting the supernatant;
(4) and (3) filtering: passing the supernatant through a membrane with aperture of 1 μm, and filtering under operation pressure of 0.15MPa to obtain yeast/rice fermentation product filtrate.
In the following examples, the yeast/zinc fermentation product was prepared as follows:
(1) preparing materials: preparing yeast fermentation bacteria liquid with the concentration of 110 cfu/mL; preparing a fermentation medium: based on 100 percent of the total mass of the fermentation medium, 1 percent of peptone and 4 percent of glucose, and the balance of distilled water;
(2) fermentation: mixing the bacterial liquid, zinc sulfate and fermentation medium, adjusting pH to 6, and fermenting in a shaker at 30 deg.C and 100r/min for 40h to obtain fermentation liquid;
(3) centrifuging: centrifuging the fermentation liquor for 30min under the conditions of 5000r/min and the centrifugal radius of 10cm, removing the precipitate, and collecting the supernatant;
(4) and (3) filtering: filtering the supernatant through a membrane with the aperture of 1 μm under the operation pressure of 0.15MPa to obtain the yeast/zinc fermentation product.
In the following examples, the yeast lysate of the second split yeast fermentation product was prepared as follows:
(1) preparing materials: preparing a saccharomyces bifidus fermentation broth with the concentration of 110 cfu/mL; preparing a fermentation medium: based on the total mass of the fermentation medium as 100 percent: 2% of glucose, 1% of peptone, 0.5% of beef extract powder, 1% of yeast extract, 0.5% of anhydrous sodium acetate, 800.1% of tween-800, 0.2% of diammonium hydrogen citrate, 0.2% of dipotassium phosphate, 0.058% of magnesium sulfate heptahydrate, 0.019% of manganese sulfate monohydrate, 0.1% of L-cysteine hydrochloride and the balance of water;
(2) fermentation: mixing the above bacterial liquid and fermentation medium, adjusting pH to 7, and fermenting in a shaker at 40 deg.C and 100r/min for 10h to obtain fermentation liquid;
(3) centrifuging: centrifuging the fermentation liquor for 30min under the conditions of 5000r/min and the centrifugal radius of 10cm, removing the supernatant, and taking the precipitate to obtain bacterial sludge;
(4) emulsification: adding trehalose into the bacterial sludge, wherein the mass ratio of the bacterial sludge to the trehalose is 7:3, and fully emulsifying by using a high-speed stirrer to obtain an emulsion;
(5) and (3) sterilization: and (3) carrying out high-temperature moist heat sterilization on the emulsion at 110 ℃ for 20min to obtain the lysate of the yeast fermentation product of the second split yeast.
In the following examples, the hamamelis virginiana extract was prepared by the following method:
(1) weighing Hamamelis virginiana, crushing to 100 meshes, adding 10 times of water, adding boric acid, extracting at 65 deg.C for 1.5h, filtering, collecting supernatant, adding citric acid to adjust pH to 3.6, extracting at room temperature for 22h, adding hydrochloric acid to adjust pH to 3.6, heating to 80 deg.C, and keeping the temperature for 32 min;
(2) filtering the mixture obtained in the step (1) to obtain a precipitate, adding an ethanol solution with the volume fraction of 70% into the precipitate, extracting at 75 ℃ for 1.5h, and filtering to obtain filter residue and a supernatant; collecting supernatant, concentrating, and drying to obtain first extract; adding the filter residue into 3 times of 80% ethanol solution by volume, performing ultrasonic extraction for 50min at the ultrasonic power of 300W, filtering, collecting supernatant, concentrating, and drying to obtain a second extract;
(3) mixing: mixing the first extract and the second extract obtained in the step (2) to obtain the Hamamelis virginiana extract.
Example 1
This example provides a skin purple reducing composition comprising, in parts by weight: 3 parts of yeast/rice fermentation product filtrate, 3 parts of yeast/zinc fermentation product, 3 parts of schizosaccharomyces cerevisiae fermentation product lysate and 3 parts of hamamelis virginiana extract.
The preparation method comprises the following steps: mixing yeast/rice fermentation product filtrate, yeast/zinc fermentation product, yeast schizosaccharomyces cerevisiae fermentation product lysate and Hamamelis virginiana extract at 40 deg.C at 300r/min speed, stirring for 20min, and filtering with 500 mesh microporous filter to obtain the composition for reducing skin purpurin.
Example 2
This example provides a skin purple reducing composition comprising, in parts by weight: 3 parts of yeast/rice fermentation product filtrate, 4 parts of yeast/zinc fermentation product, 1 part of schizosaccharomyces cerevisiae fermentation product lysate and 3 parts of hamamelis virginiana extract.
The preparation method comprises the following steps: mixing yeast/rice fermentation product filtrate, yeast/zinc fermentation product, yeast schizosaccharomyces cerevisiae fermentation product lysate and Hamamelis virginiana extract at 35 deg.C at 400r/min speed, stirring for 30min, and filtering with 400 mesh microporous filter to obtain the composition for reducing skin purpurin.
Example 3
This example provides a skin purple reducing composition comprising, in parts by weight: 1 part of yeast/rice fermentation product filtrate, 4 parts of yeast/zinc fermentation product, 2 parts of schizosaccharomyces cerevisiae fermentation product lysate and 2 parts of hamamelis virginiana extract.
The preparation method comprises the following steps: mixing yeast/rice fermentation product filtrate, yeast/zinc fermentation product, yeast schizosaccharomyces cerevisiae fermentation product lysate and Hamamelis virginiana extract at 45 deg.C and 200r/min speed, stirring for 10min, and filtering with 600 mesh microporous filter to obtain the composition for reducing skin purpurin.
Example 4
This example provides a skin purple reducing composition comprising, in parts by weight: 0.1 part of yeast/rice fermentation product filtrate, 5 parts of yeast/zinc fermentation product, 5 parts of schizosaccharomyces cerevisiae fermentation product lysate and 0.1 part of hamamelis virginiana extract.
The preparation method comprises the following steps: mixing yeast/rice fermentation product filtrate, yeast/zinc fermentation product, yeast schizosaccharomyces cerevisiae fermentation product lysate and Hamamelis virginiana extract at 40 deg.C at 300r/min speed, stirring for 20min, and filtering with 500 mesh microporous filter to obtain the composition for reducing skin purpurin.
Example 5
This example provides a skin purple reducing composition comprising, in parts by weight: 5 parts of yeast/rice fermentation product filtrate, 0.1 part of yeast/zinc fermentation product, 0.1 part of schizosaccharomyces cerevisiae fermentation product lysate and 5 parts of hamamelis virginiana extract.
The preparation method comprises the following steps: mixing yeast/rice fermentation product filtrate, yeast/zinc fermentation product, yeast schizosaccharomyces cerevisiae fermentation product lysate and Hamamelis virginiana extract at 40 deg.C at 300r/min speed, stirring for 20min, and filtering with 500 mesh microporous filter to obtain the composition for reducing skin purpurin.
Comparative example 1
This comparative example provides a skin purple reducing composition which differs from example 1 only in that it does not contain the yeast/rice fermentation product filtrate, the yeast/zinc fermentation product content is increased to 4 parts, the diaschisis yeast fermentation product lysate content is increased to 4 parts, and the hamamelis virginiana extract content is increased to 4 parts.
Comparative example 2
This comparative example provides a skin purple reducing composition, differing from example 1 only in that the composition did not contain yeast/zinc fermentation product, the yeast/rice fermentation product filtrate content was increased to 4 parts, the yeast dehiscence yeast fermentation product lysate content was increased to 4 parts, and the hamamelis virginiana extract content was increased to 4 parts.
Comparative example 3
This comparative example provides a skin purple reducing composition, differing from example 1 only in that the composition did not contain a yeast dehiscence yeast ferment lysate, the yeast/rice ferment filtrate content was increased to 4 parts, the yeast/zinc ferment content was increased to 4 parts, and the hamamelis virginiana extract content was increased to 4 parts.
Comparative example 4
This comparative example provides a skin purple reducing composition, differing from example 1 only in that the composition did not contain hamamelis virginiana extract, the yeast/rice fermentation product filtrate content was increased to 4 parts, the yeast/zinc fermentation product content was increased to 4 parts, and the yeast schizosaccharomyces fermentation product lysate content was increased to 4 parts.
Test example 1
Haemolysis test of erythrocytes
The erythrocyte hemolysis test is one of the alternatives of the rabbit eye irritation test (Draize test), and the basic principle is to evaluate the damage of chemicals to eye tissue cells by measuring the dissolution amount and denaturation degree of hemoglobin. The RBC test is used internationally for evaluating eye irritation studies on chemicals such as cosmetics and raw materials.
The purpurin-reducing compositions prepared in examples 1-5 and the purpurin-reducing compositions prepared in comparative examples 1-4 were subjected to a red blood cell hemolysis test according to the RBC test protocol and grading standards of the European Alternatives validation Process (ECVAM). ECVAM. ECVAM DB-ALM INVITOX Protoco, Red blood cells testsystem INVITOX No.37 Ispra. Italy ECVAM,1994 ]. The specific test method is shown in table 1:
TABLE 1
L/D
|
Grading
|
﹥100
|
Has no irritation
|
10﹤L/D≤100
|
Micro-stimulation property
|
1﹤L/D≤10
|
Mild irritation
|
0.1﹤L/D≤1
|
Moderate irritation
|
L/D≤0.1
|
Severe irritation |
Results of erythrolysis experiments of the purpurin-reducing compositions prepared in examples 1 to 5 and the purpurin-reducing compositions prepared in comparative examples 1 to 4 are shown in Table 2, in which HC50 is the sample concentration at which 50% of erythrocytes are hemolyzed, DI is the protein denaturation index, and L/D is the ratio of HC50 to DI.
TABLE 2
As can be seen from the results of the experiments on hemolysis of erythrocytes in Table 2, the compositions for reducing skin purpurin prepared in examples 1-5 of the present invention all had L/D values of more than 100, and the irritation was classified as non-irritating, indicating that the compositions for reducing skin purpurin prepared in the present invention all had the characteristics of being mild and non-irritating, and that the sample concentration HC of 50% of erythrocytes in the compositions for reducing skin purpurin prepared in examples 1-5 was hemolyzed50HC's substantially greater than the skin purple reducing compositions prepared in comparative examples 1-450Meanwhile, the protein denaturation index DI is obviously smaller than that of the composition for reducing the skin purpurin prepared in comparative examples 1-4, which shows that the four components of the composition are scientifically and reasonably compatible, and the toxic and side effects and the irritation can be obviously reduced after the synergistic effect is achieved, so that the aims of weakening the cell hemolysis degree and reducing the protein denaturation are fulfilled.
Test example 2
In vitro oil control efficacy evaluation test
Test samples: the purpurin reducing compositions prepared in examples 1-5 and the purpurin reducing compositions prepared in comparative examples 1-4.
Subject: liver of 3 male SD rats
Experimental procedure 9 identical 5 α -reducing enzyme solutions were prepared from liver homogenates of 3 male SD rats, and divided into 9 groups, and one of the composition samples was added to each group, and the inhibition rate of the oil control composition containing the control composition on the 5 α -reducing enzyme solution was measured through a series of reaction systems, and the higher the inhibition rate is, the better the oil control effect is, and the test results are shown in Table 3.
TABLE 3
As can be seen from Table 3, the inhibition ratio of the skin purpurin reducing composition prepared in examples 1-5 to 5 α -reductase solution is generally higher than that of the composition prepared in comparative examples 1-4, and the inhibition ratio to 5 α -reductase solution is between 33.20-60.32%, wherein the inhibition ratio of example 1 is the highest and reaches 60.32%, so that the oil control effect is the best.
Test example 3
Pathogenic bacteria inhibition test
The examples and comparative examples were tested for the effect and effect on the production of a flora in the presence of both probiotics and pathogens. The skin purple-reducing compositions prepared in examples 1 to 5 and the skin purple-reducing compositions prepared in comparative examples 1 to 4 were prepared at a mass concentration of 0.5%, respectively. The colonies were then incubated in the above medium and the number of bacteria was counted after 24 hours. The flora is a mixed flora of Micrococcus keiskei and Staphylococcus aureus, and the specific test results are shown in Table 4 (wherein T24/T0 represents the bacterial growth multiple within 24 hours).
TABLE 4
As can be seen from the test data in Table 4, the skin purple reducing compositions prepared in examples 1-5 showed significantly less pathogenic bacteria after 24 hours of incubation than the compositions of comparative examples 1-4. This indicates that the skin purple reducing composition of the present invention can significantly reduce the number of pathogenic bacteria due to lack of food and nutrition, and has the effects of selectively being utilized by probiotics and inhibiting the pathogenic bacteria, thereby fundamentally inhibiting the metabolism of bacteria producing skin purple. Moreover, compared with comparative examples 1 to 4, the skin purple reducing compositions prepared in examples 1 to 5 have a larger reduction amount of pathogenic bacteria, which shows that the skin purple reducing compositions provided by the invention can effectively inhibit the breeding and metabolism of pathogenic bacteria through reasonable collocation and synergy of the four active ingredients.
Test example 4
Purple testing of skin image analysis System VISIA
90 subjects were selected and divided into 9 groups of 10 subjects, and the compositions for reducing skin purple prepared in examples 1 to 5 and the compositions for reducing skin purple prepared in comparative examples 1 to 4 were tried for 2 weeks, respectively, and before trial, after trial for 1 week and after trial for 2 weeks, photographs were taken using the skin image analysis system VISIA, purple characteristic counts were recorded, and the average value was calculated, and the specific test results were as described in table 5:
TABLE 5
As can be seen from the test data in Table 5, the number of purple characteristic of human face was significantly reduced in subjects after 1-2 weeks using the skin purple reducing compositions prepared in examples 1-5, and the number of purple characteristic was reduced to 11 or less after 2 weeks of trial. Compared with the compositions prepared by using comparative examples 1-4, the composition for reducing the skin purpurin has the advantages that the number of the facial purpurin characteristics of human faces is obviously reduced, the effect is stable, and the repeated phenomenon cannot occur after long-time use, so that the composition for reducing the skin purpurin has the synergistic effect by reasonably matching the four active ingredients, and can effectively reduce the skin purpurin.
In addition, fig. 1 is a diagram showing the characteristics of the purple pigment of the face before trial use of the subject. Fig. 2 is a facial purple profile of a subject 1 week after using the skin purple reducing composition prepared in example 1. Fig. 3 is a facial purple profile of a subject 2 weeks after using the skin purple reducing composition prepared in example 1. It can also be clearly seen that the number of human facial purpurin features is greatly reduced after the composition for reducing skin purpurin is used, and the effect is basically the same between 1 week and 2 weeks of the test, so that the problem of purpurin is completely cured without repeated appearance.
The applicant states that the present invention is illustrated by the above examples of the skin purple reducing composition of the present invention and the preparation method and application thereof, but the present invention is not limited to the above examples, i.e. it does not mean that the present invention must be implemented by the above examples. It should be understood by those skilled in the art that any modification of the present invention, equivalent substitutions of the raw materials of the product of the present invention, addition of auxiliary components, selection of specific modes, etc., are within the scope and disclosure of the present invention.