CN110823660A - Tissue floating slice paving device and method for floating slice paving - Google Patents
Tissue floating slice paving device and method for floating slice paving Download PDFInfo
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- CN110823660A CN110823660A CN201911172393.3A CN201911172393A CN110823660A CN 110823660 A CN110823660 A CN 110823660A CN 201911172393 A CN201911172393 A CN 201911172393A CN 110823660 A CN110823660 A CN 110823660A
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- 238000000034 method Methods 0.000 title claims abstract description 14
- 238000011534 incubation Methods 0.000 claims abstract description 8
- 238000005452 bending Methods 0.000 claims abstract description 4
- 239000008055 phosphate buffer solution Substances 0.000 claims description 14
- 239000011521 glass Substances 0.000 claims description 11
- 238000004061 bleaching Methods 0.000 claims description 9
- 239000007788 liquid Substances 0.000 claims description 7
- 239000000243 solution Substances 0.000 claims description 4
- 239000006059 cover glass Substances 0.000 claims description 3
- 239000002184 metal Substances 0.000 claims description 3
- 239000003153 chemical reaction reagent Substances 0.000 description 4
- 238000004043 dyeing Methods 0.000 description 4
- 230000000694 effects Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 230000000877 morphologic effect Effects 0.000 description 2
- 241000251468 Actinopterygii Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 238000011532 immunohistochemical staining Methods 0.000 description 1
- 230000004807 localization Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000011002 quantification Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000009870 specific binding Effects 0.000 description 1
- 239000010902 straw Substances 0.000 description 1
Images
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/2813—Producing thin layers of samples on a substrate, e.g. smearing, spinning-on
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N1/31—Apparatus therefor
- G01N1/312—Apparatus therefor for samples mounted on planar substrates
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Engineering & Computer Science (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The invention provides a tissue floating piece spreading device which comprises a handle part and two branch parts symmetrically arranged at the lower end of the handle part, wherein anti-skid grains are arranged on two sides of the handle part, each branch part comprises a sheet-shaped end connected with the handle part and a cylindrical end integrally connected with the sheet-shaped end, a distance adjusting structure is arranged between the two sheet-shaped ends of the two branch parts, the diameter of the cylindrical end is 0.3mm, and the cylindrical end can be bent. The invention also provides a method for carrying out floating slice paving by the tissue floating slice paving device, which comprises the steps of bending the cylindrical end of the tissue floating slice paving device into different angles and different lengths according to tissue slice incubation containers with different sizes and different depths; the distance between the two branch parts is adjusted by adjusting the position locked by the nut on the bolt according to the size of the tissue slice to be processed; the floating slide applicator and its operation of the present invention cause the tissue sections to not be completely collapsed together, allowing the tissue sections to lay flat on the slide.
Description
Technical Field
The invention belongs to the technical field of medical instruments, and particularly relates to a tissue floating slice laying device and a method for floating slice laying by using the same.
Background
In biomedical research, immunohistochemical staining techniques are one of the most commonly used methods in morphological studies by observing the tissue structure of tissue sections under a microscope and the localization and quantification of specific proteins based on the principle of antigen-antibody specific binding. The method is favorable for fully combining the reagent and the tissue section, and has better dyeing effect than the method that the tissue section is stuck on a glass slide. However, how to remove the tissue section from the reagent and to spread it on a glass slide is the most critical step to be able to finally observe under a microscope and obtain a satisfactory morphological picture.
The tissue slices are in microns, typically between 10-30 microns thick, and it is very difficult to remove such thin tissue slices from the reagent and lay them very flat on glass slides. At present, researchers use a variety of methods, for example, a soft small brush pen, a suction tube, a small syringe needle, and the like are bent into a small hook to fish up a tissue slice, and for a tissue slice with a larger size, a container for floating the slice is also larger, and the tissue slice is generally fished up by using the brush pen; and for the small tissue, the small tissue is sucked by a straw, or the tip of a syringe is subtracted, the head is bent, and a small hook is formed for fishing out the tissue slice. However, in either case, the tissue sections shrink together after being pulled out of the reagent. Although the tissue is returned to the liquid and developed again, the phosphate buffer solution dropped on the glass slide is limited, and it is very difficult to develop a tissue section flat on the glass slide, and it is more difficult to develop a tissue section larger. These methods all result in tissue slices that curl or even collapse together when they are scooped, making it difficult to spread flat on a glass slide. Slices are also easily damaged when adjusted to lay flat on a slide, which, although seemingly a small operation, is the most critical step in the success of an experiment. If this step is not performed well, even if the dyeing effect is good, a good-quality dyeing image cannot be obtained, and the early experiment is also wasted, so that a special tissue bleaching sheet spreader is required to solve the above problems.
Disclosure of Invention
The invention aims to solve the technical problem of providing a tissue floating slice paving device and a method for floating slice paving thereof, which are used for solving the problems in the background art.
In order to solve the technical problem, an embodiment of the invention provides a tissue floating piece paving device which is characterized by comprising a handle part and two branch parts symmetrically arranged at the lower end of the handle part, wherein anti-skid threads are arranged on two sides of the handle part, each branch part comprises a sheet-shaped end connected with the handle part and a cylindrical end integrally connected with the sheet-shaped end, a distance adjusting structure is arranged between the two sheet-shaped ends of the two branch parts, the diameter of the cylindrical end is 0.3mm, and the cylindrical end can be bent.
Further, the distance adjusting structure comprises a bolt and a nut, through holes for the bolt to pass through are formed in the two sheet-shaped ends, the distance between the two sheet-shaped ends of the two branch parts is adjusted through the nut, and a connecting spring is sleeved on the inner side part, opposite to the inner side part, of the two branch parts of the bolt.
Furthermore, the cylindrical end in the branch part of the tissue floating sheet paving sheet is made of deformation memory metal.
Furthermore, the width of the side surface of the sheet end gradually narrows from top to bottom to form an isosceles trapezoid structure, and the thickness of the sheet end gradually thins from top to bottom.
Wherein the thickness of the joint of the flaky end and the cylindrical end is 0.3mm, and the thickness of the upper end of the flaky part is 0.5-0.8 mm.
Further, the height of the cylindrical end is 20 mm.
Further, the height of the sheet part is 40 mm.
Preferably, the bolt is mounted at an intermediate position of the sheet part.
The embodiment of the invention also provides a method for using the tissue floating slice paving device for floating slice paving, which is characterized by comprising the following steps:
(1) bending the cylindrical end of the tissue floating device into different angles and different lengths according to tissue slice incubation containers with different sizes and different depths; the distance between the two branch parts is adjusted by adjusting the position locked by the nut on the bolt according to the size of the tissue slice to be processed;
(2) an operator holds the handle part by hand, extends the two branch parts of the tissue floating slice spreading device into the incubation container, uses the cylindrical end to support the tissue slices so that the tissue slices can not be completely shrunk together, and then moves the tissue slices onto a phosphate buffer solution dripped on a glass slide; the tissue slices are enabled to float on the phosphate buffer solution in a relatively flat way by means of the tension of the liquid;
(3) and drawing out the tissue floating slice spreading device, slowly sucking the phosphate buffer solution from the periphery by using a liquid moving device, completely and flatly spreading the tissue slices, dripping the mounting solution after sucking the phosphate buffer solution, and mounting the slices by using a cover glass.
The technical scheme of the invention has the following beneficial effects: according to the tissue floating slice laying device, the cylindrical end can be bent into two sections with different lengths and different angles; a distance adjusting structure is arranged between the two sheet-shaped ends of the branch part, and the distance between the sheet-shaped ends can be adjusted through the distance adjusting structure; after the length adjustment and the distance adjustment, extending a tissue floating slice spreading device into the incubation container to lift a tissue slice; the floating slide plate-laying device and the operation thereof enable the tissue sections not to be completely shrunk together, so that the tissue sections are flatly laid on the glass slide and then placed on the glass slide on which the phosphate buffer solution is dripped, and the tissue sections are more easily unfolded, thereby shooting a good-quality dyeing image.
Drawings
FIG. 1 is a schematic structural view of the present invention;
FIG. 2 is a side view of the present invention;
fig. 3 is a schematic view of the structure of the cylindrical end of the present invention after being bent with respect to the sheet-like end.
Description of reference numerals: 1. a handle portion; 11. anti-skid lines; 2. a branching section; 21. a blade end; 22. a cylindrical end; 3. a bolt; 4. a nut; 5. a spring is connected.
Detailed Description
In order to make the technical problems, technical solutions and advantages of the present invention more apparent, the following detailed description is given with reference to the accompanying drawings and specific embodiments.
In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", "front", "rear", and the like indicate orientations or positional relationships based on those shown in the drawings, and are only for convenience of description and simplicity of description, but do not indicate or imply that the referred device or element must have a specific orientation, be constructed in a specific orientation, and be operated, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
In the description of the present invention, it should be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.
As shown in fig. 1-3, a tissue floating slice spreading device comprises a handle part 1 and two branch parts 2 symmetrically arranged at the lower end of the handle part 1, wherein anti-slip threads 11 are arranged at two sides of the handle part 1, the branch parts 2 comprise sheet-shaped ends 21 connected with the handle part 1 and cylindrical ends 22 integrally connected with the sheet-shaped ends 21, a distance adjusting structure is arranged between the two sheet-shaped ends 21 of the two branch parts 2, the diameter of the cylindrical ends 22 is 0.3mm, and the cylindrical ends 22 can be bent.
In a further embodiment, the distance adjusting structure includes a bolt 3 and a nut 4, through holes for the bolt 3 to pass through are formed in the two sheet-shaped ends 21, the distance between the two sheet-shaped ends 21 of the two branch portions 2 is adjusted by locking the position of the nut 4, a connecting spring 5 is sleeved on the opposite inner portion of the two branch portions 2 of the bolt 3, and the connecting spring 5 increases the elasticity between the two branch portions 2, so that the two branch portions 2 are in a separated state; and then the distance between the two branch parts 2 is adjusted by locking the nut 4 of the distance adjusting device on the bolt 3, so that the two branch parts 2 form a stable distance.
In a further embodiment, the cylindrical end 22 of the branch part 2 of the tissue floating sheet paving sheet is made of a deformation memory metal, and the cylindrical end 22 of the branch part 2 is bent at different positions and different angles according to requirements, so that two sections with different lengths and different angles are formed.
In a further embodiment, the width of the side surface of the sheet end 21 gradually narrows from top to bottom to form an isosceles trapezoid structure, and the thickness of the sheet end 21 gradually thins from top to bottom.
Wherein the thickness of the joint of the sheet end 21 and the cylindrical end 22 is 0.3mm, and the thickness of the upper end of the sheet part is 0.5-0.8 mm.
In a further embodiment, the height of the cylindrical end 22 is 20 mm.
In a further embodiment, the height of the tab 21 is 40 mm.
Preferably, the bolt 3 is mounted at an intermediate position of the sheet portion 21.
Embodiments of the invention additionally provide a method for tissue bleaching tile paving by using the tissue bleaching tile paving device, which comprises the following steps:
(1) bending the cylindrical end 22 of the tissue floating device into different angles and different lengths according to tissue slice incubation containers with different sizes and different depths; the distance between the two branch parts 2 is adjusted through the position locked by the adjusting nut 4 on the bolt 3 according to the size of the tissue slice to be processed;
(2) an operator holds the handle part 1 by hand, extends the two branch parts 2 of the tissue floating slice spreading device into the incubation container, uses the cylindrical end 22 to lift the tissue slices so that the tissue slices can not be completely shrunk together, and then moves the tissue slices onto a glass slide dropped phosphate buffer solution; the tissue slices are enabled to float on the phosphate buffer solution in a relatively flat way by means of the tension of the liquid;
(3) and drawing out the tissue floating slice spreading device, slowly sucking the phosphate buffer solution from the periphery by using a liquid moving device, completely and flatly spreading the tissue slices, dripping the mounting solution after sucking the phosphate buffer solution, and mounting the slices by using a cover glass.
While the foregoing is directed to the preferred embodiment of the present invention, it will be understood by those skilled in the art that various changes and modifications may be made without departing from the spirit and scope of the invention as defined in the appended claims.
Claims (9)
1. The utility model provides a piece ware is spread to tissue float piece which characterized in that includes handle portion and two branches portion that the symmetry was established at handle portion lower extreme, the both sides of handle portion are provided with anti-skidding line, branch portion include the slice end that is connected with handle portion and hold an organic whole with the slice and be connected the cylinder end, two be provided with a distance between the bivalve form end of branch portion and adjust the structure, the diameter of cylinder end is 0.3mm, the cylinder end can be buckled.
2. The tissue floating slice spreading device according to claim 1, wherein the distance adjusting structure comprises a bolt and a nut, a through hole for the bolt to pass through is arranged on the two slice ends, the distance between the two slice ends of the two branch parts is adjusted through the nut, and a connecting spring is sleeved on the opposite inner side part of the two branch parts of the bolt.
3. The tissue floating slice paving device according to claim 1, wherein the cylindrical end of the branch part of the tissue floating slice paving device is made of a deformation memory metal.
4. The tissue floating slice spreading device according to claim 3, wherein the width of the lateral surface of the slice-shaped end gradually narrows from top to bottom to form an isosceles trapezoid structure, and the thickness of the slice-shaped end gradually thins from top to bottom.
5. The tissue bleaching sheet spreader according to claim 4, wherein the thickness of the joint between the sheet-like end and the cylindrical end is 0.3mm, and the thickness of the upper end of the sheet-like part is 0.5-0.8 mm.
6. The tissue bleaching sheet spreader of claim 1, wherein the height of the cylindrical end is 20 mm.
7. The tissue bleaching sheet paver of claim 1, characterized in that the height of said sheet is 40 mm.
8. The tissue bleaching sheet paver of claim 2, characterized in that the bolt is mounted at the middle position of the sheet part.
9. A method of using a tissue bleaching slide paver according to any one of claims 1-8 for bleaching slide paving, comprising the steps of:
(1) bending the cylindrical end of the tissue floating device into different angles and different lengths according to tissue slice incubation containers with different sizes and different depths; the distance between the two branch parts is adjusted by adjusting the position locked by the nut on the bolt according to the size of the tissue slice to be processed;
(2) an operator holds the handle part by hand, extends the two branch parts of the tissue floating slice spreading device into the incubation container, uses the cylindrical end to support the tissue slices so that the tissue slices can not be completely shrunk together, and then moves the tissue slices onto a phosphate buffer solution dripped on a glass slide; the tissue slices are enabled to float on the phosphate buffer solution in a relatively flat way by means of the tension of the liquid;
(3) and drawing out the tissue floating slice spreading device, slowly sucking the phosphate buffer solution from the periphery by using a liquid moving device, completely and flatly spreading the tissue slices, dripping the mounting solution after sucking the phosphate buffer solution, and mounting the slices by using a cover glass.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911172393.3A CN110823660A (en) | 2019-11-26 | 2019-11-26 | Tissue floating slice paving device and method for floating slice paving |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911172393.3A CN110823660A (en) | 2019-11-26 | 2019-11-26 | Tissue floating slice paving device and method for floating slice paving |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110823660A true CN110823660A (en) | 2020-02-21 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201911172393.3A Pending CN110823660A (en) | 2019-11-26 | 2019-11-26 | Tissue floating slice paving device and method for floating slice paving |
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102426124A (en) * | 2011-11-08 | 2012-04-25 | 中国人民解放军第四军医大学 | Device of pulling and flatting section for pathologic tissue frozen section and method thereof |
| CN205003003U (en) * | 2015-09-11 | 2016-01-27 | 安徽省农业科学院茶叶研究所 | Instrument of moving is chosen with tissue to paraffin section |
| CN205483738U (en) * | 2016-03-23 | 2016-08-17 | 西安交通大学 | Cell ultrathin section creep plate drags for piece needle |
| CN105973635A (en) * | 2016-06-29 | 2016-09-28 | 廖丹丹 | Mobile device used in preparation process of biological tissue sample slices |
| CN206414321U (en) * | 2016-10-25 | 2017-08-18 | 黄河科技学院 | Return hook-type tweezers |
| CN109187118A (en) * | 2018-07-09 | 2019-01-11 | 中南大学湘雅医院 | Hard tissue pathological section unfolding method and device |
-
2019
- 2019-11-26 CN CN201911172393.3A patent/CN110823660A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102426124A (en) * | 2011-11-08 | 2012-04-25 | 中国人民解放军第四军医大学 | Device of pulling and flatting section for pathologic tissue frozen section and method thereof |
| CN205003003U (en) * | 2015-09-11 | 2016-01-27 | 安徽省农业科学院茶叶研究所 | Instrument of moving is chosen with tissue to paraffin section |
| CN205483738U (en) * | 2016-03-23 | 2016-08-17 | 西安交通大学 | Cell ultrathin section creep plate drags for piece needle |
| CN105973635A (en) * | 2016-06-29 | 2016-09-28 | 廖丹丹 | Mobile device used in preparation process of biological tissue sample slices |
| CN206414321U (en) * | 2016-10-25 | 2017-08-18 | 黄河科技学院 | Return hook-type tweezers |
| CN109187118A (en) * | 2018-07-09 | 2019-01-11 | 中南大学湘雅医院 | Hard tissue pathological section unfolding method and device |
Non-Patent Citations (1)
| Title |
|---|
| 孙宝元等: "《现代执行器技术》", 30 April 2003, 吉林大学出版社 * |
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Application publication date: 20200221 |