CN110734327A - liquid fermented biological foliar fertilizer and preparation method thereof - Google Patents
liquid fermented biological foliar fertilizer and preparation method thereof Download PDFInfo
- Publication number
- CN110734327A CN110734327A CN201910934997.0A CN201910934997A CN110734327A CN 110734327 A CN110734327 A CN 110734327A CN 201910934997 A CN201910934997 A CN 201910934997A CN 110734327 A CN110734327 A CN 110734327A
- Authority
- CN
- China
- Prior art keywords
- parts
- bacillus
- solution
- cgmcc
- liquid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D9/00—Other inorganic fertilisers
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Inorganic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Fertilizers (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses liquid fermentation biological foliar fertilizer and a preparation method thereof, wherein the biological foliar fertilizer comprises the following components, by weight, 1000 parts of yeast fermentation powder slag 2000-portions, 500 parts of starch 1000-portions, 500 parts of corn flour 1000-portions, 600 parts of rice bran 1000-portions, 50-200 parts of diatomite, 10-20 parts of bacillus fermentation liquid, 5-20 parts of trace element solution, 1-20 parts of 20% potassium fulvate solution, 10-20 parts of 0.1% seaweed extract solution and 200 parts of 50% gelatin solution 100-portions.
Description
Technical Field
The invention relates to the field of fertilizers, in particular to liquid fermented biological foliar fertilizer and a preparation method thereof.
Background
The way that plants absorb nutrient elements is mainly divided into root absorption and leaf surface absorption, and the nutrition mode that nutrients are absorbed through nutrient organs except roots is called as extra-root nutrition of plants. The method of applying fertilizer to the surface of a nutrient body other than roots is called topdressing, also called foliar fertilization. Fertilizers used for foliar fertilization are then called foliar fertilizers.
The foliar fertilizer is applied to the leaf surface of a plant, is directly absorbed by the leaves of the plant, does not need to pass through soil and roots, and reduces the loss of nutrient components, so the foliar fertilizer has the characteristics of high fertilizer efficiency utilization rate, quick nutrient absorption, strong pertinence, simple and easy application method and the like, and can also make up the influence caused by the decline of the roots on the plants with the root functions beginning to decline in the mature period.
However, the existing foliar fertilizer is generally used, has incomplete nutrition and insufficient organic matters, and can not effectively prevent diseases.
Disclosure of Invention
The invention aims to provide liquid fermented biological foliar fertilizer and a preparation method thereof aiming at the defects of the prior art, the method adopts a liquid fermentation technology, bacillus fermentation liquor and a plurality of organic components are uniformly mixed and then fermented, and inorganic nutrient components are added to finally form microbial fertilizers with comprehensive nutrition.
In order to achieve the above object, the present invention is achieved by the following technical solutions.
liquid fermentation biological foliar fertilizer, which is characterized by comprising the following components, by weight, 1000 portions of yeast fermentation powder slag 2000-portions, 500 portions of starch 1000-portions, 500 portions of corn flour 1000-portions, 600 portions of rice bran 1000-portions, 50-200 portions of diatomite, 10-20 portions of bacillus fermentation broth, 5-20 portions of trace element solution, 1-20 portions of 20% potassium fulvate solution, 10-20 portions of 0.1% seaweed extract solution, and 100 portions of 50% gelatin solution 200-portions;
the bacillus fermentation liquor comprises bacillus methylotrophicus with the preservation number of CGMCC NO. 18268; bacillus methylotrophicus with the preservation number of CGMCC NO. 18269; bacillus amyloliquefaciens with the preservation number of CGMCC NO. 18263.
A method for preparing a biological foliar fertilizer by liquid fermentation, which comprises the following steps:
step 1, inoculating three functional strains of bacillus methylotrophicus with the preservation number of CGMCC NO.18268, bacillus methylotrophicus with the preservation number of CGMCC NO.18269 and bacillus amyloliquefaciens with the preservation number of CGMCC NO.18263 into a liquid fermentation culture medium respectively under the aseptic condition, culturing for 24-36h for activation under the condition of 25-37 ℃, then inoculating into another liquid fermentation culture medium again according to the inoculation proportion of 10-20 Wt%, and performing expanded culture for 24-36h under the condition of 25-37 ℃;
step 2, respectively preparing a bacillus methylotrophicus liquid, a bacillus methylotrophicus liquid and a bacillus amyloliquefaciens liquid;
step 3, uniformly mixing the bacterial liquid prepared in the step 2 according to the volume ratio of 1-5: 1-5 to prepare bacillus fermentation liquid;
step 4, adding 10-20 parts of the bacillus fermentation liquor prepared in the step 3 into a nutrient source, adding a proper amount of water until the raw materials are submerged for 10cm, introducing sterile air, stirring, fermenting, and fermenting for 10-20 days at the temperature of 25-37 ℃;
step 5, filtering the product obtained in the step 4 through a 400-mesh screen to obtain a filtrate, namely a stock solution A;
step 6, uniformly mixing a 20% potassium fulvate solution, a trace element solution, a 0.1% seaweed extract solution and a 50% gelatin solution according to the proportion to obtain a stock solution B;
and 7, uniformly mixing the stock solution A prepared in the step 5 with the stock solution B prepared in the step 6 to obtain the liquid fermented microbial foliar fertilizer.
In step , the liquid fermentation medium in step 1 contains parts by weight of peptone 10g/L, yeast extract 5g/L, and sodium chloride 10 g/L.
, the nutrient source in the step 4 comprises the following components, by weight, 1000-2000 parts of yeast fermentation powder slag, 500-1000 parts of starch, 500-1000 parts of corn flour, 600-1000 parts of rice bran and 50-200 parts of diatomite.
, the microelement solution in step 6 comprises the following components, by weight, 0.5-1% of sugar alcohol calcium, 0.1-1% of sugar alcohol boron, 0.04-0.5% of sugar alcohol molybdenum, 0.1-1% of amino acid chelated iron, 0.5-3% of amino acid chelated zinc, 0.5-1.5% of amino acid chelated magnesium, 0.04-0.5% of citric acid chelated copper, 0.5-2% of citric acid chelated manganese, and the balance water.
, the 50% gelatin solution in step 6 is prepared by weighing corresponding gelatin according to weight ratio, and stirring and dissolving in warm water at 40-60 deg.C.
Bacillus methylotrophicus (Bacillus methylotrophicus) with the preservation number of CGMCC NO.18268 and the preservation date: 22/7/2019, deposit address: xilu No.1 Hospital No. 3, Beijing, Chaoyang, the department of deposit: china general microbiological culture Collection center;
bacillus amyloliquefaciens (Bacillus amyloliquefaciens) with the preservation number of CGMCC NO.18263 and the preservation date: 22/7/2019, deposit address: xilu No.1 Hospital No. 3, Beijing, Chaoyang, the department of deposit: china general microbiological culture Collection center;
bacillus methylotrophicus (Bacillus methylotrophicus) with the preservation number of CGMCC NO.18269 and the preservation date: 22/7/2019, deposit address: xilu No.1 Hospital No. 3, Beijing, Chaoyang, the department of deposit: china general microbiological culture Collection center.
The invention has the beneficial effects that:
1. compared with the prior art, the invention adopts a liquid fermentation mode, and the working procedure is simple;
2. the invention adds a plurality of microelements and nutrients needed by plants, and has comprehensive nutrition;
3. the trace elements added in the invention are all chelating elements, so that the fertilizer is easy to be absorbed by crops, the influence on the environment due to nutrient loss can be reduced in the application process, the application amount can be reduced, and the cost can be reduced.
3. The functional bacillus flora is added, so that the stress resistance is enhanced, and the powdery mildew is prevented and treated;
4. the raw materials adopted by the invention contain industrial and agricultural wastes, so that the industrial and agricultural wastes are fully utilized, and the sustainable development is facilitated.
Detailed Description
The present invention is further illustrated in with reference to the following examples, which are not intended to be limiting.
A liquid fermentation microbial foliar fertilizer is characterized by comprising the following raw materials in parts by weight:
1000 portions of yeast fermentation powder slag, 1000 portions of starch, 500 portions of corn flour, 1000 portions of rice bran, 600 portions of diatomite, 50-200 portions of bacillus fermentation liquor (bacillus methylotrophicus, bacillus methylotrophicus and bacillus amyloliquefaciens), 10-20 portions of trace element solution, 1-20 portions of 20 mass percent potassium fulvate solution, 10-20 portions of 0.1 mass percent seaweed extract solution and 100 portions of 50 mass percent gelatin solution.
The preparation method of the microbial foliar fertilizer comprises the following steps:
(1) respectively inoculating Bacillus methylotrophicus (CGMCC NO. 18268), Bacillus methylotrophicus (Bacillus subtilis) CGMCC NO.18269 and Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) CGMCC NO.18263 functional strains in a liquid fermentation culture medium under the aseptic condition, culturing for 24-36h for activation at 25-37 ℃, then inoculating again in the liquid fermentation culture medium according to the inoculation ratio of 10-20 Wt%, and performing amplification culture for 24-36h at 25-37 ℃;
(2) respectively preparing bacillus methylotrophicus liquid, bacillus methylotrophicus liquid and bacillus amyloliquefaciens liquid;
(3) uniformly mixing the bacterial liquid prepared in the step (2) according to the volume ratio of 1-5: 1-5 to prepare a bacillus fermentation liquid;
(4) adding 10-20 parts of the bacillus fermentation liquor prepared in the step (3) into a nutrient source, adding a proper amount of water until the raw material is submerged for 10cm, introducing sterile air, stirring, fermenting, and fermenting for 10-20 days at 25-37 ℃;
(5) filtering the product obtained in the step (4) through a 400-mesh screen to obtain a filtrate, namely a stock solution 1;
(6) uniformly mixing a 20% potassium fulvate solution, a trace element solution, a 0.1% seaweed extract solution and a 50% gelatin solution according to the proportion to obtain a stock solution 2;
(7) and (4) uniformly mixing the stock solution 1 prepared in the step (5) and the stock solution 2 prepared in the step (6) to obtain the liquid fermented microbial foliar fertilizer.
The preparation method of the liquid fermentation medium in the step (1) comprises the following steps:
10g/L of peptone, 5g/L of yeast extract and 10g/L of sodium chloride.
The nutrient source components in the step (4) are as follows:
1000-2000 parts of yeast fermentation powder slag, 500-1000 parts of starch, 500-1000 parts of corn flour, 600-1000 parts of rice bran and 50-200 parts of diatomite.
The trace element solution in the step (6) comprises the following components:
0.5-1% of sugar alcohol calcium, 0.1-1% of sugar alcohol boron, 0.04-0.5% of sugar alcohol molybdenum, 0.1-1% of amino acid chelated iron, 0.5-3% of amino acid chelated zinc, 0.5-1.5% of amino acid chelated magnesium, 0.04-0.5% of citric acid chelated copper and 0.5-2% of citric acid chelated manganese.
The 50% gelatin solution in the step (6) is prepared as follows:
weighing corresponding gelatin according to the weight ratio, and placing the gelatin in warm water at the temperature of 40-60 ℃ to be stirred and dissolved for later use.
Detailed description of the preferred embodiment 1
biological foliar fertilizer is prepared from the following raw materials, by weight (kg), 1000 parts of yeast fermentation powder residues, 500 parts of starch, 500 parts of corn flour, 600 parts of rice bran, 50 parts of diatomite, 10 parts of bacillus fermentation liquor (bacillus methylotrophicus, bacillus methylotrophicus and bacillus amyloliquefaciens), 20 parts of trace element solution, 1 part of 20% potassium fulvate solution, 10 parts of 0.1% seaweed extract solution and 100 parts of 50% gelatin solution.
The preparation method of the biological foliar fertilizer comprises the following specific steps:
(1) respectively inoculating Bacillus methylotrophicus (Bacillus methylotrophicus) CGMCC NO.18268, Bacillus methylotrophicus (Bacillus subtilis) CGMCC NO.18269 and Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) CGMCC NO.18263 functional strains in a liquid fermentation culture medium under the aseptic condition, culturing for 36h activation at 37 ℃, then inoculating the strains in the liquid fermentation culture medium again according to the inoculation ratio of 10 Wt%, and performing amplification culture for 36h at 37 ℃;
(2) respectively preparing bacillus methylotrophicus liquid, bacillus methylotrophicus liquid and bacillus amyloliquefaciens liquid;
(3) uniformly mixing the bacterial liquid prepared in the step (2) according to the volume ratio of 3:4:3 to prepare bacillus fermentation liquid;
(4) adding 15 parts of the bacillus fermentation liquor prepared in the step (3) into a nutrient source, adding a proper amount of water until the raw materials are submerged for 10cm, introducing sterile air, stirring, fermenting, and fermenting for 15 days at 37 ℃;
(5) filtering the product obtained in the step (4) through a 400-mesh screen to obtain a filtrate, namely a stock solution A;
(6) uniformly mixing a 20% potassium fulvate solution, a trace element solution, a 0.1% seaweed extract solution and a 50% gelatin solution according to the proportion to obtain a stock solution B;
(7) and (4) uniformly mixing the stock solution A prepared in the step (5) with the stock solution B prepared in the step (6) to obtain the liquid fermented microbial foliar fertilizer.
The preparation method of the liquid fermentation medium comprises the following steps:
10g/L of peptone, 5g/L of yeast extract and 10g/L of sodium chloride
The nutrient source comprises the following components:
1000 parts of yeast fermentation powder residues, 500 parts of starch, 500 parts of corn flour, 600 parts of rice bran and 50 parts of diatomite.
The trace element solution comprises the following components:
0.5% of sugar alcohol calcium, 0.1% of sugar alcohol boron, 0.5% of sugar alcohol molybdenum, 1% of amino acid chelated iron, 3% of amino acid chelated zinc, 0.5% of amino acid chelated magnesium, 1.5% of citric acid chelated copper and 0.5% of citric acid chelated manganese.
The 50% gelatin solution was prepared as follows:
weighing corresponding gelatin according to the weight ratio, and placing the gelatin in warm water at 40 ℃ to be stirred and dissolved for later use.
Specific example 2
biological foliar fertilizer is prepared from 2000 parts (kg) of yeast fermentation powder residues, 1000 parts of starch, 1000 parts of corn flour, 1000 parts of rice bran, 200 parts of diatomite, 20 parts of bacillus fermentation liquor (bacillus methylotrophicus, bacillus methylotrophicus and bacillus amyloliquefaciens), 5 parts of trace element solution, 20 parts of 20% potassium fulvate solution, 20 parts of 0.1% seaweed extract solution and 200 parts of 50% gelatin solution.
The preparation method of the biological foliar fertilizer comprises the following specific steps:
(1) respectively inoculating Bacillus methylotrophicus (Bacillus methylotrophicus) CGMCC NO.18268, Bacillus methylotrophicus (Bacillus subtilis) CGMCC NO.18269 and Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) CGMCC NO.18263 functional strains in a liquid fermentation culture medium under the aseptic condition, culturing for 36h activation at 37 ℃, then inoculating the strains in the liquid fermentation culture medium again according to the inoculation ratio of 10 Wt%, and performing amplification culture for 36h at 37 ℃;
(2) respectively preparing a methylotrophic bacillus liquid, a bacillus subtilis liquid and a bacillus amyloliquefaciens liquid;
(3) uniformly mixing the bacterial liquid prepared in the step (2) according to the volume ratio of 4:3:3 to obtain bacillus fermentation liquid;
(4) adding 20 parts of the bacillus fermentation liquor prepared in the step (3) into a nutrient source, adding a proper amount of water until the raw materials are submerged for 10cm, introducing sterile air, stirring, fermenting, and fermenting for 15 days at 37 ℃;
(5) filtering the product obtained in the step (4) through a 400-mesh screen to obtain a filtrate, namely a stock solution A;
(6) uniformly mixing a 20% potassium fulvate solution, a trace element solution, a 0.1% seaweed extract solution and a 50% gelatin solution according to the proportion to obtain a stock solution B;
(7) and (4) uniformly mixing the stock solution A prepared in the step (5) with the stock solution B prepared in the step (6) to obtain the liquid fermented microbial foliar fertilizer.
The preparation method of the liquid fermentation medium comprises the following steps:
10g/L of peptone, 5g/L of yeast extract and 10g/L of sodium chloride
The nutrient source comprises the following components:
2000 parts of yeast fermentation powder residues, 1000 parts of starch, 1000 parts of corn flour, 1000 parts of rice bran and 200 parts of diatomite.
The trace element solution comprises the following components:
1% of sugar alcohol calcium, 1% of sugar alcohol boron, 0.04% of sugar alcohol molybdenum, 0.1% of amino acid chelated iron, 0.5% of amino acid chelated zinc, 1.5% of amino acid chelated magnesium, 0.04% of citric acid chelated copper and 2% of citric acid chelated manganese.
The 50% gelatin solution was prepared as follows:
weighing corresponding gelatin according to the weight ratio, and stirring and dissolving the gelatin at 60 ℃ for later use.
Specific example 3
The biological foliar fertilizer is prepared from 1500 parts (kg) of yeast fermentation powder residues, 750 parts of starch, 750 parts of corn flour, 800 parts of rice bran, 125 parts of diatomite, 15 parts of bacillus fermentation liquor (bacillus methylotrophicus, bacillus methylotrophicus and bacillus amyloliquefaciens), 12 parts of trace element solution, 11 parts of 20% potassium fulvate solution, 15 parts of 0.1% seaweed extract solution and 150 parts of 50% gelatin solution.
The preparation method of the biological foliar fertilizer comprises the following specific steps:
(1) respectively inoculating Bacillus methylotrophicus (Bacillus methylotrophicus) CGMCC NO.18268, Bacillus methylotrophicus (Bacillus subtilis) CGMCC NO.18269 and Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) CGMCC NO.18263 functional strains in a liquid fermentation culture medium under the aseptic condition, culturing for 36h activation at 37 ℃, then inoculating the strains in the liquid fermentation culture medium again according to the inoculation ratio of 10 Wt%, and performing amplification culture for 36h at 37 ℃;
(2) respectively preparing a methylotrophic bacillus liquid, a bacillus subtilis liquid and a bacillus amyloliquefaciens liquid;
(3) uniformly mixing the bacterial liquid prepared in the step (2) according to the volume ratio of 2:1:1 to obtain bacillus fermentation liquid;
(4) adding 20 parts of the bacillus fermentation liquor prepared in the step (3) into a nutrient source, adding a proper amount of water until the raw materials are submerged for 10cm, introducing sterile air, stirring, fermenting, and fermenting for 15 days at 37 ℃;
(5) filtering the product obtained in the step (4) through a 400-mesh screen to obtain a filtrate, namely a stock solution 1;
(6) uniformly mixing a 20% potassium fulvate solution, a trace element solution, a 0.1% seaweed extract solution and a 50% gelatin solution according to the proportion to obtain a stock solution 2;
(7) and (4) uniformly mixing the stock solution 1 prepared in the step (5) and the stock solution 2 prepared in the step (6) to obtain the liquid fermented microbial foliar fertilizer.
The preparation method of the liquid fermentation medium comprises the following steps:
10g/L of peptone, 5g/L of yeast extract and 10g/L of sodium chloride
The nutrient source comprises the following components:
1500 parts of yeast fermentation powder residues, 750 parts of starch, 750 parts of corn flour, 800 parts of rice bran and 125 parts of diatomite.
The trace element solution comprises the following components:
the trace element solution comprises the following components: 0.7% of sugar alcohol calcium, 0.6% of sugar alcohol boron, 0.3% of sugar alcohol molybdenum, 0.5% of amino acid chelated iron, 1.2% of amino acid chelated zinc, 1% of amino acid chelated magnesium, 0.25% of citric acid chelated copper and 1.3% of citric acid chelated manganese.
The 50% gelatin solution was prepared as follows:
weighing corresponding gelatin according to the weight ratio, and placing the gelatin in warm water at 50 ℃ to be stirred and dissolved for later use.
Examples effects of field application
Under the same environment, 120 nodulizing lettuce pots are planted in a greenhouse by adopting the same planting management method and are randomly divided into 4 groups, 10 plants in each group are respectively numbered as a test group 1, a test group 2, a test group 3 and a control group, wherein the test groups 1, 2 and 3 are respectively sprayed on the surface of a plant by adopting the biological foliar fertilizer of examples 1-3, the control group is sprayed with common water, each test is repeated for 3 times, the specific spraying mode is that times are sprayed every 7 days after transplantation, the application amount is that the biological foliar fertilizer is diluted by 200 times of water and is sprayed on the surface of a plant leaf blade by 300 times, the agronomic characters of each group are collected at the later nodulizing stage for comparative analysis, and the specific results are shown in table 1:
TABLE 1 agricultural trait survey results of lettuce
| Number of blades | Fresh weight of overground part (g) | Fresh weight of underground (g) | |
| Test group 1 | 9.4 | 80-100(86.3) | 8.5-10(9.2) |
| Test group 2 | 9.8 | 80-100(98.2) | 8.5-10(10.6) |
| Test group 3 | 10.1 | 80-100(95.6) | 8.5-10(9.8) |
| Control group | 9.2 | 60-70(64.7) | 8(8.4) |
As can be seen from the above table 1, the biological foliar fertilizer disclosed by the invention is applied to the planting of head lettuce, and has a remarkable effect of improving the yield of lettuce.
It should be understood that the above-described embodiments of the present invention are merely examples for clearly illustrating the present invention, and are not intended to limit the embodiments of the present invention. Other variations and modifications will be apparent to persons skilled in the art in light of the above description. Not all embodiments are exhaustive. All obvious changes and modifications which are obvious to the technical scheme of the invention are covered by the protection scope of the invention.
Claims (9)
1, liquid fermentation biological foliar fertilizer, which is characterized by comprising the following components, by weight, 1000 parts of yeast fermentation powder slag 2000-portions, 500 parts of starch 1000-portions, 500 parts of corn flour 1000-portions, 600 parts of rice bran 1000-portions, 50-200 parts of diatomite, 10-20 parts of bacillus fermentation liquid, 5-20 parts of trace element solution, 1-20 parts of 20% potassium fulvate solution, 10-20 parts of 0.1% seaweed extract solution and 100 parts of 50% gelatin solution 200-portions;
the bacillus fermentation liquor comprises bacillus methylotrophicus with the preservation number of CGMCC NO. 18268; bacillus methylotrophicus with the preservation number of CGMCC NO. 18269; bacillus amyloliquefaciens with the preservation number of CGMCC NO. 18263.
2. The kinds of liquid fermented biological foliar fertilizer of claim 1, characterized in that it comprises the following components, by weight, 1000 parts of yeast fermentation powder residues, 500 parts of starch, 500 parts of corn flour, 600 parts of rice bran, 50 parts of diatomite, 10 parts of bacillus fermentation liquor, 5 parts of trace element solution, 1 part of 20% potassium fulvate solution, 10 parts of 0.1% seaweed extract solution, and 100 parts of 50% gelatin solution;
the bacillus fermentation liquor comprises bacillus methylotrophicus with the preservation number of CGMCC NO. 18268; bacillus methylotrophicus with the preservation number of CGMCC NO. 18269; bacillus amyloliquefaciens with the preservation number of CGMCC NO. 18263.
3. The kinds of liquid fermented biological foliar fertilizer of claim 1, wherein the foliar fertilizer comprises, by weight, 2000 parts of yeast fermentation powder residues, 1000 parts of starch, 1000 parts of corn flour, 1000 parts of rice bran, 200 parts of diatomaceous earth, 20 parts of bacillus fermentation broth, 20 parts of trace element solution, 20 parts of 20% potassium fulvate solution, 20 parts of 0.1% seaweed extract solution, and 200 parts of 50% gelatin solution;
the bacillus fermentation liquor comprises bacillus methylotrophicus with the preservation number of CGMCC NO. 18268; bacillus methylotrophicus with the preservation number of CGMCC NO. 18269; bacillus amyloliquefaciens with the preservation number of CGMCC NO. 18263;
the microbial inoculum in the bacillus fermentation liquor comprises bacillus methylotrophicus with the preservation number of CGMCC NO. 18268; bacillus methylotrophicus with the preservation number of CGMCC NO. 18269; bacillus amyloliquefaciens with the preservation number of CGMCC NO. 18263.
4. The kinds of liquid fermented biological foliar fertilizer as claimed in claim 1, which is characterized in that it comprises the following components, by weight, 1500 parts of yeast fermentation powder residues, 750 parts of starch, 750 parts of corn flour, 800 parts of rice bran, 120 parts of diatomite, 15 parts of bacillus fermentation liquor, 12 parts of trace element solution, 11 parts of 20% potassium fulvate solution, 15 parts of 0.1% seaweed extract solution, and 150 parts of 50% gelatin solution;
the bacillus fermentation liquor comprises bacillus methylotrophicus with the preservation number of CGMCC NO. 18268; bacillus methylotrophicus with the preservation number of CGMCC NO. 18269; bacillus amyloliquefaciens with the preservation number of CGMCC NO. 18263.
5. The preparation method of kinds of liquid fermented biological foliar fertilizer according to claim 1 is characterized by comprising the following steps:
step 1, inoculating three functional strains of bacillus methylotrophicus with the preservation number of CGMCC NO.18268, bacillus methylotrophicus with the preservation number of CGMCC NO.18269 and bacillus amyloliquefaciens with the preservation number of CGMCC NO.18263 into a liquid fermentation culture medium respectively under the aseptic condition, culturing for 24-36h for activation under the condition of 25-37 ℃, then inoculating into another liquid fermentation culture medium again according to the inoculation proportion of 10-20 Wt%, and performing expanded culture for 24-36h under the condition of 25-37 ℃;
step 2, respectively preparing a bacillus methylotrophicus liquid, a bacillus methylotrophicus liquid and a bacillus amyloliquefaciens liquid;
step 3, uniformly mixing the bacterial liquid prepared in the step 2 according to the volume ratio of 1-5: 1-5 to prepare bacillus fermentation liquid;
step 4, adding 10-20 parts of the bacillus fermentation liquor prepared in the step 3 into a nutrient source, adding a proper amount of water until the raw materials are submerged for 10cm, introducing sterile air, stirring, fermenting, and fermenting for 10-20 days at the temperature of 25-37 ℃;
step 5, filtering the product obtained in the step 4 through a 400-mesh screen to obtain a filtrate, namely a stock solution A;
step 6, uniformly mixing a 20% potassium fulvate solution, a trace element solution, a 0.1% seaweed extract solution and a 50% gelatin solution according to the proportion to obtain a stock solution B;
and 7, uniformly mixing the stock solution A prepared in the step 5 with the stock solution B prepared in the step 6 to obtain the liquid fermented microbial foliar fertilizer.
6. The preparation method of kinds of liquid fermented biological foliar fertilizer as claimed in claim 5, wherein the liquid fermentation medium in step 1 contains weight portions of peptone 10g/L, yeast extract 5g/L, sodium chloride 10 g/L.
7. The preparation method of liquid fermentation biological foliar fertilizer as claimed in claim 5, wherein the nutrient source in step 4 comprises the following components, by weight, 1000 parts of yeast fermentation powder slag 2000 parts, 1000 parts of starch 500-.
8. The preparation method of kinds of liquid fermented biological foliar fertilizer according to claim 5, wherein the trace element solution in step 6 comprises the following components, by weight, 0.5-1% of sugar alcohol calcium, 0.1-1% of sugar alcohol boron, 0.04-0.5% of sugar alcohol molybdenum, 0.1-1% of amino acid chelated iron, 0.5-3% of amino acid chelated zinc, 0.5-1.5% of amino acid chelated magnesium, 0.04-0.5% of citric acid chelated copper, 0.5-2% of citric acid chelated manganese, and the balance water.
9. The preparation method of kinds of liquid fermented biological foliar fertilizer according to claim 5, wherein the 50% gelatin solution in step 6 is prepared by weighing corresponding gelatin according to the weight ratio, and stirring and dissolving in warm water at 40-60 ℃ for later use.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910934997.0A CN110734327A (en) | 2019-09-29 | 2019-09-29 | liquid fermented biological foliar fertilizer and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910934997.0A CN110734327A (en) | 2019-09-29 | 2019-09-29 | liquid fermented biological foliar fertilizer and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110734327A true CN110734327A (en) | 2020-01-31 |
Family
ID=69269803
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910934997.0A Pending CN110734327A (en) | 2019-09-29 | 2019-09-29 | liquid fermented biological foliar fertilizer and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110734327A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112552103A (en) * | 2020-11-30 | 2021-03-26 | 云南省微生物发酵工程研究中心有限公司 | Microbial agent containing biochemical fulvic acid potassium and preparation method thereof |
| CN115362924A (en) * | 2022-08-22 | 2022-11-22 | 江西省中国科学院庐山植物园 | Method for promoting aquatic plant to take root in gravel environment |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102746064A (en) * | 2012-07-11 | 2012-10-24 | 德州市元和农业科技开发有限责任公司 | Microbial foliage fertilizer, and preparation method and application thereof |
| CN105837291A (en) * | 2016-04-21 | 2016-08-10 | 深圳市芭田生态工程股份有限公司 | Microorganism activating agent specially used for microorganism liquid fertilizer and preparation method thereof |
| CN108440146A (en) * | 2018-05-30 | 2018-08-24 | 云南云天化股份有限公司 | A kind of full trophic function type suspension liquid fertilizer of high concentration and preparation method thereof |
-
2019
- 2019-09-29 CN CN201910934997.0A patent/CN110734327A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102746064A (en) * | 2012-07-11 | 2012-10-24 | 德州市元和农业科技开发有限责任公司 | Microbial foliage fertilizer, and preparation method and application thereof |
| CN105837291A (en) * | 2016-04-21 | 2016-08-10 | 深圳市芭田生态工程股份有限公司 | Microorganism activating agent specially used for microorganism liquid fertilizer and preparation method thereof |
| CN108440146A (en) * | 2018-05-30 | 2018-08-24 | 云南云天化股份有限公司 | A kind of full trophic function type suspension liquid fertilizer of high concentration and preparation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 黎肇家等: "甲基营养型芽孢杆菌对黄柏苗的促生作用", 《湖南农业科学》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112552103A (en) * | 2020-11-30 | 2021-03-26 | 云南省微生物发酵工程研究中心有限公司 | Microbial agent containing biochemical fulvic acid potassium and preparation method thereof |
| CN115362924A (en) * | 2022-08-22 | 2022-11-22 | 江西省中国科学院庐山植物园 | Method for promoting aquatic plant to take root in gravel environment |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107794240B (en) | Bacillus megaterium, polypeptide agricultural microbial agent thereof, and preparation method and application thereof | |
| CN102816006B (en) | Special apple microorganism bacterial manure and method for preparing same | |
| CN110183266A (en) | A kind of small molecule alginic acid microbial manure | |
| CN105130685A (en) | Preparation method for organic fertilizer containing Nu-polyglutamic acid | |
| CN107580818B (en) | A kind of integrated approach of soil conditioning and reparation | |
| CN109650986A (en) | It is a kind of improve degeneration vegetable soil charcoal base biological organic fertilizer and its application | |
| CN113214000A (en) | Composite microbial fertilizer and preparation method thereof | |
| CN102533553A (en) | Organic matter decomposition combined fungicide for rapidly degrading rice straws and application method thereof | |
| CN113501734A (en) | Gamma-polyglutamic acid and chitosan oligosaccharide combined water-soluble fertilizer | |
| CN105237058A (en) | Bio-organic fertilizer and preparation method thereof | |
| CN111607526B (en) | Straw decomposition agent and preparation method and application thereof | |
| CN112225595A (en) | Water-soluble compound microbial fertilizer and preparation method thereof | |
| CN114478109A (en) | Biological foliar fertilizer and preparation method thereof | |
| CN110734327A (en) | liquid fermented biological foliar fertilizer and preparation method thereof | |
| CN110981635A (en) | Agricultural microbial agent with nitrogen fixation, phosphorus dissolution, potassium dissolution and cellulose dissolution functions and preparation method thereof | |
| CN105198512B (en) | A kind of method that double-microbial fermentation prepares gamma-polyglutamic acid organic fertilizer | |
| CN110606782A (en) | Farmland soil water-retaining organic fertilizer and preparation method thereof | |
| CN109913234A (en) | A kind of soil conditioner and its application | |
| CN111470899B (en) | Special organic liquid fertilizer for sugar beet and application of special organic liquid fertilizer in sugar beet planting | |
| CN105315068A (en) | Composition for promoting crop growth and preparation method and fertilization method thereof | |
| CN108821858A (en) | The disease-resistant dedicated bacterial manure of dragon fruit, preparation method and applications | |
| CN109438079B (en) | Soluble biological organic fertilizer and production method thereof | |
| CN107974423B (en) | Soil biological activator and preparation method thereof | |
| CN111039718A (en) | Agricultural streptomyces microflavus composite bacterial fertilizer taking enzymolysis cassava residues as base material and preparation method thereof | |
| CN115819123A (en) | Biofertilizer for improving yield of winter wheat re-sowed corn and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20200131 |
|
| RJ01 | Rejection of invention patent application after publication |