CN110521874A - A kind of method of EPA and DHA deposition in promotion chicken - Google Patents

A kind of method of EPA and DHA deposition in promotion chicken Download PDF

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Publication number
CN110521874A
CN110521874A CN201910796957.4A CN201910796957A CN110521874A CN 110521874 A CN110521874 A CN 110521874A CN 201910796957 A CN201910796957 A CN 201910796957A CN 110521874 A CN110521874 A CN 110521874A
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epa
diet
chicken
dha
isoflavones
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Inventor
苟钟勇
崔小燕
蒋守群
李龙
范秋丽
林厦菁
王一冰
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Institute of Animal Science of Guangdong Academy of Agricultural Sciences
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/02—Breeding vertebrates
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/116—Heterocyclic compounds
    • A23K20/121—Heterocyclic compounds containing oxygen or sulfur as hetero atom
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/158—Fatty acids; Fats; Products containing oils or fats
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/70—Feeding-stuffs specially adapted for particular animals for birds
    • A23K50/75—Feeding-stuffs specially adapted for particular animals for birds for poultry
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
    • Y02P60/87—Re-use of by-products of food processing for fodder production

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  • Life Sciences & Earth Sciences (AREA)
  • Polymers & Plastics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Animal Husbandry (AREA)
  • Zoology (AREA)
  • Food Science & Technology (AREA)
  • Physiology (AREA)
  • Mycology (AREA)
  • Molecular Biology (AREA)
  • Botany (AREA)
  • Biotechnology (AREA)
  • Health & Medical Sciences (AREA)
  • Environmental Sciences (AREA)
  • Birds (AREA)
  • Animal Behavior & Ethology (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)

Abstract

The invention discloses a kind of methods of EPA and DHA deposition in promotion chicken, using the diet feeding dorking containing alpha-linolenic acid, and isoflavones are added in diet.It can promote the synthesis of EPA and DHA by the addition of isoflavones, so that EPA and DHA are effectively deposited in chicken, and then improve value of chicken, so that people can absorb EPA and DHA by edible chicken.

Description

A kind of method of EPA and DHA deposition in promotion chicken
Technical field
The present invention relates to broiler fodder technical field, EPA and DHA deposition in more particularly to a kind of promotion chicken Method.
Background technique
EPA (Eicosapentaenoic Acid) i.e. eicosapentaenoic acid belongs to the serial polyunsaturated fatty acid of ω -3, It is the indispensable important nutrient of human body, the content of cholesterol and triglycerides can be reduced, promotes internal saturated fatty acid generation It thanks, the cardiovascular diseases such as prevention cerebral thrombosis, cerebral hemorrhage, hypertension.
DHA (Docosahexaenoic Acid) i.e. docosahexaenoic acid is nervous system cell growth and is maintained A kind of main component is the important composition ingredient of brain and retina.
In order to supplement EPA, DHA, people generally select EPA, DHA health care product taken by purification, however, as EPA With the deep sea fish oil and marine algae of DHA main source, due to fish overfishing, marine environmental pollution, yield and quality Degradation is no longer satisfied ever-increasing market demand, therefore, still needs to develop the more natural intake side EPA, DHA Formula.
Summary of the invention
In view of this, improving chicken nutriture value the invention discloses a kind of method of EPA and DHA deposition in promotion chicken Value, and then by edible chicken, that is, ingestible EPA, DHA, and EPA the and DHA bioavailability of food source is higher.
To achieve the goals above, the present invention adopts the following technical scheme:
Application of the isoflavones in promoting chicken in EPA and DHA deposition.
Isoflavones promotes the application in chicken in the diet or diet additive of EPA and DHA deposition in preparation.
A kind of method of EPA and DHA deposition in promotion chicken, using the diet feeding dorking containing alpha-linolenic acid, and Isoflavones is added in diet.
Isoflavones have different physiological roles, while alpha-linolenic acid (ALA) as n-3 precursor have synthesis EPA and DHA ability (mainly realizes) that the addition of isoflavones may be by changing one by unsaturated enzyme and extension enzyme Serial enzyme activity effect, promotes the efficiency of ALA Synthesis EPA and DHA and is deposited on it effectively in chicken.
Preferably, using the diet feeding dorking containing linseed oil, and isoflavones is added in diet；Linseed Alpha-linolenic acid is rich in oil.
The diet of EPA and DHA deposition in a kind of promotion chicken, linseed oil content is 2%-4% in diet；Linseed oil Middle alpha-linolenic acid content is 50-60%；Isoflavones additive amount is 20-60mg/kg in diet.
It can be seen via above technical scheme that compared with prior art, the invention discloses EPA in a kind of promotion chicken and The method of DHA deposition, promotes the synthesis of EPA and DHA by the addition of isoflavones, so that EPA and DHA are effectively deposited on chicken In meat, value of chicken is improved, so that people can absorb EPA and DHA by edible chicken.
Specific embodiment
The technical scheme in the embodiments of the invention will be clearly and completely described below, it is clear that described implementation Example is only a part of the embodiment of the present invention, instead of all the embodiments.Based on the embodiments of the present invention, this field is common Technical staff's every other embodiment obtained without making creative work belongs to the model that the present invention protects It encloses.
Embodiment 1
It selects the fast large-scale Lingnan yellow broiler (public affairs) of 900 29 ages in days (597 ± 0.00g of weight BW) to be used as test chicken, presses Diet ingredient difference is divided into 5 processing groups (table 1), 6 repetitions of every processing, 30 chickens of each repetition.Test chicken ground is flat to support, often (30) raisings are repeated in a column, particulate material and drinking-water is freely eaten.It is carried out according to conventinal breeding operating instruction and immune programme It raises and immune；Experimental period 29-66 age in days.
Table 1
Test uses corn-soybean meal Basic drawing, trophic level Primary Reference " fowl raising standard " (NY/T 33- 2004) be arranged, diet composition and trophic level calculated according to Chinese feed ingredient and nutritive value table (2018), diet composition and As shown in table 2, diet fatty acid composition is as shown in table 3 for trophic level (air bells dry basis).Each group diet is made using granulator Pellet.
Table 2
Wherein,1Premix is that every kilogram of diet adds respectively:
Vitamin B1: 2.4mg (29-49 age in days), 2.1mg (50-66 age in days)；
Vitamin B2: 4mg (29-49 age in days), 3mg (50-66 age in days)；
Niacin: 17mg (29-49 age in days), 15mg (50-66 age in days)；
Choline: 600mg (29-49 age in days), 500mg (50-66 age in days)；
Vitamin A: 6000IU；Vitamin D3: 500IU；Vitamin E: 20IU；Vitamin K3: 0.50mg；Pantothenic acid: 10mg； Vitamin B6: 3.5mg；Biotin: 0.15mg；Folic acid: 0.55:mg；Vitamin B12: 0.01mg；
Fe:80mg；Cu:7mg；Mn:60mg；Zn:75mg；I:0.35mg；Se:0.23mg is (respectively with ferrous sulfate monohydrate FeSO4·H2O, cupric sulfate pentahydrate CuSO4·5H2O, manganese sulfate monohydrate MnSO4·H2O, monohydrate zinc sulphate ZnSO4·H2O, acid iodide Calcium Ca (IO3)2·H2O and sodium selenite Na2SeO3Form addition).
2Trophic level is calculated value, with reference to Database of Feed (2018).
In table, LA group diet contains 4% lard；LA+LO group diet+2% linseed oil containing 2% lard；LO group diet contains 4% Linseed oil；SI indicates 30mg/kg isoflavones.
In addition to adding SI, remaining ingredient forms phase with LA+LO group and LO group diet respectively for LA+LO+SI group and LO+SI group Together, SI is added in premix.
Synthesizing isoflavones, (Synthetic isoflavone, SI, main component is 6- methyl -2- ethyl -7- propionyl Oxygroup isoflavones, 98%) purity is greater than to be provided by Xinnandu Feed Science & Technology Co., Ltd., Guangdong.
The specific Component Percent (measured value) of linseed oil (Linseed oil, LO): alpha-linolenic acid (C18:3n-3) 58.3%, oleic acid (C18:1n-9) 17.1%, linoleic acid (C18:2n-6) 14.0%, stearic acid (C18:0) 4.0%, palmitinic acid (C16:0) 5.1%, etc..
The specific Component Percent (measured value) of lard (Lard, LA): oleic acid (C18:1n-9) 40.2%, palmitinic acid (C16: 0) 23%, linoleic acid (C18:2n-6) 15.2%, stearic acid (C18:0) 14.1%, palmitoleic acid (C16:1n-7) 2.7%, meat Myristic acid (C14:0) 1.3%, alpha-linolenic acid (C18:3n-3) 1.5%, etc..
Table 3
Note: the lower fatty acid of content is unlisted.
It when 66 age in days, is weighed again after fracture 12 hours to calculate average daily gain (ADG), is with each every repetition of processing Unit record average daily gain (ADFI) calculates feed-weight ratio (F/G).2 are then chosen from the every repetition of each processing close to flat The test chicken of equal weight carries out quiet vein blood sampling using anticoagulant heparin pipe；1200g is centrifuged 10 minutes separated plasma samples at 4 DEG C Product, and -80 DEG C are stored in after blood plasma is in charge of until analysis.Broiler chicken acquires left pectoral sample after butchering, and moves on to -20 DEG C of refrigerators It saves, is used for chest muscle content of fatty acid and composition measuring.
Plasma Biochemical analysis: it is determined always using commercial reagent box (Nanjing Jian Cheng Bioengineering Research Institute) spectrophotometry Superoxide dismutase (T-SOD) activity, total antioxidant capacity (T-AOC), reduced glutathione (GSH), oxidized form paddy Guang Sweet peptide (GSSG) and malonaldehyde (MDA) content.Then calculate GSH/GSSG ratio.Using automatic biochemistry analyzer (Holland Selectra-proxl clinical chemistry systems) and matched reagent box (Zhongsheng Beikong Biological Science & Technology Co., Ltd.) measurement blood plasma In total triglycerides (TG), total cholesterol (TCH), high-density lipoprotein cholesterol (HDL-C) and low density lipoprotein cholesterol (LDL-C) concentration.
Content of fatty acid is measured with composition using gas chromatography (GC): accurately weighing 2.0g chest muscle meat sample freeze-dried powder In hydrolysis pipe, 4mL chloracetyl-methanol solution (1:10) and the interior target hendecoic acid methyl esters of 1mL C11:0 (1mg/ml) is added Solution covers tightly bottle cap and is placed in 2 hours of 80 DEG C of water-baths.7% potassium carbonate 5mL is added after taking out cooling, shaking is uniform, with 0.2um filter membrane, which is crossed, after the speed centrifugation 5min of 1200r/min is packed into sample introduction bottle, upper machine testing.
It is carried out using one-way ANOVA in SAS (version 9.1, SAS Institute, Cary, NC) statistical software The data analysis of five processing groups, data are missed (SEM) with average value ± average and are indicated, linseed oil substitution level and soybean The reciprocation of isoflavones is analyzed using GLM, using P < 0.05 as significance of difference judgment criteria.
1. growth performance
As shown in table 4, compared with LA diet, half is substituted with linseed oil or whole lards significantly reduce yellow-feather broiler Average daily gain (ADFI), completely substitution reduce F/G；The whole BW and ADG of 29-66 age in days chicken be not by significant shadow It rings.LO group diet improves 4.56% (P < 0.05) compared to the feed efficiency of LA group diet.
Table 4
Wherein,The standard error of SEM:5 processing group, n=6 (every group of 6 repetitions, 2 chickens of each repetition).
a,bColleague indicates that difference is not significant (P > 0.05) without letter, different significant P < 0.05 of lowercase letter indication difference).
1BW, weight；ADG, average daily gain；ADFI, average daily gain；F/G, feed-weight ratio.
2Refer to the P value of 5 processing group one-way ANOVA analysis.
3LO indicates that linseed oil dosage, linseed oil substitute half or whole lards.
2. blood plasma is anti-oxidant and lipid-metabolism index
As shown in table 5, half is substituted with linseed oil or whole lards do not make significant difference to broiler chicken blood plasma Antioxidant Indexes (P>0.05).When SI is added to linseed oil diet, LO+SI diet group broiler chicken blood plasma T-SOD activity is significantly higher than LO and LA+ LO group.Five kinds of diets do not make significant difference to blood plasma T-AOC, MDA, GSH and GSSG content, but the diet group (LA+LO+SI containing SI And LO+SI) GSH/GSSG ratio be above LA+LO and LO group (P < 0.05).Compared with LA group, in linseed oil diet TG and TCH content is substantially reduced, and especially in the presence of SI, TG and TCH level can be further decreased.Linseed oil substitutes pig There are significant interactions for the influence of oil level and SI to plasma TC H content, but each diet contains chicken plasma HDL-C or LDL-C Amount does not make significant difference.
Table 5
Wherein,The standard error of SEM:5 processing group, n=6 (every group of 6 repetitions, 2 chickens of each repetition).
a,b,cColleague indicates that difference is not significant (P > 0.05) without letter, different significant P < 0.05 of lowercase letter indication difference).
1T-AOC, total antioxidant capacity；T-SOD, total number born；GSH, reduced glutathione；GSSG, oxygen Change type glutathione；MDA, malonaldehyde；TG, total triglycerides；TCH, total cholesterol；HDL-C, high-density lipoprotein cholesterol； LDL-C, low density lipoprotein cholesterol.
2Refer to the P value of 5 processing group one-way ANOVA analysis.
3LO indicates that linseed oil dosage, linseed oil substitute half or whole lards.
3. chest muscle fatty acid forms
As shown in table 6, compared with LA group, ALA (C18:3n-3), the EPA (C20:5n-3) of the chicken containing linseed oil are eaten Content is higher (P < 0.05), myristic acid (C14:0), palmitinic acid (C16:0), oleic acid (C18:1n-9), arachidonic acid (C20: 4n-6) and sour (C24:0) content of tetracosa carbon is lower.The saturated fatty acid (SFA) and n- for the chicken that diet is fed containing linseed oil 6/n-3 ratio is below the chicken (p < 0.05) fed with lard merely.Compared with LA group, the n-3 fatty acid of linseed oil group contains Amount, PUFA content and PUFA/SFA ratio are higher (P < 0.05), but have no significant effect to the content of n-6 fatty acid.Work as addition When 30mg/kg SI is into the diet containing linseed oil (LA+LO and LO), EPA content further increases (P < 0.05), DHA content It dramatically increases.Show that the alpha-linolenic acid in diet can be effectively deposited in yellow-feather broiler muscle, and EPA can be effectively converted into It is deposited in chicken；Addition isoflavones can further promote alpha-linolenic acid to be converted to EPA, turn again into linseed oil diet Chemical conversion DHA is deposited in chicken.
Table 6
The standard error of SEM:5 processing group, n=6 (every group of 6 repetitions, 2 chickens of each repetition).
a,b,cColleague indicates that difference is not significant (P > 0.05) without letter, significant (the P < of different lowercase letter indication differences 0.05)。
1EPA: eicosapentaenoic acid, DHA: docosahexaenoic acid, SFA: saturated fatty acid, MUFA: monounsaturated fatty acid Acid, PUFA: polyunsaturated fatty acid, n-3: including alpha-linolenic acid, EPA, DHA, n-6: including linoleic acid, eicosadienoic acid, Eicosatrienoic acid, arachidonic acid.
2Refer to the P value of 5 processing group one-way ANOVA analysis.
3LO indicates that linseed oil dosage, linseed oil substitute half or whole lards.
In conclusion feed efficiency can be improved with linseed oil substitution half lard or whole lards, make Lingnan Yellow chicken Meat be rich in n-3 polyunsaturated fatty acid (ALA, EPA and DHA), and reduce blood lipids (plasma TG, TCH).In flax SI is added in seed oil diet and further improves the content of EPA and DHA in chest muscle, and reduces plasma TG and TCH level, Improve the antioxidant status of chicken.In terms of TCH content, linseed oil it is horizontal between SI there are significant reciprocation, Middle LO+SI diet is optimal.
In short, isoflavones has the function of promoting to be converted into EPA and DHA from ALA on the basis of having ALA, from And increase EPA and DHA content in muscle.Therefore, isoflavones and linseed oil are used cooperatively and can produce rich in n-3 not The chicken meat product of saturated fatty acid (ALA, EPA, DHA) is made contributions to meet consumer to the needs of healthy food.
The foregoing description of the disclosed embodiments enables those skilled in the art to implement or use the present invention. A variety of modifications of above-described embodiment will be readily apparent to those skilled in the art, it is as defined herein General Principle can be realized in other embodiments without departing from the spirit or scope of the present invention.Therefore, of the invention It is not intended to be limited to the embodiments shown herein, and is to fit to and the principles and novel features disclosed herein phase one The widest scope of cause.

Claims (5)

1. application of the isoflavones in promoting chicken in EPA and DHA deposition.
2. isoflavones promotes the application in chicken in the diet or diet additive of EPA and DHA deposition in preparation.
3. a kind of method for promoting EPA and DHA deposition in chicken, which is characterized in that feed meat using the diet containing alpha-linolenic acid Chicken, and isoflavones is added in diet.
4. a kind of method for promoting EPA and DHA deposition in chicken according to claim 1, which is characterized in that using containing Asia The diet feeding dorking of flaxseed oil, and isoflavones is added in diet；Contain alpha-linolenic acid in the linseed oil.
5. a kind of diet for promoting EPA and DHA deposition in chicken, which is characterized in that linseed oil content is 2- in the diet 4%；Alpha-linolenic acid content is 50-60% in the linseed oil；Isoflavones additive amount is 20-60mg/ in the diet kg。
CN201910796957.4A 2019-08-27 2019-08-27 A kind of method of EPA and DHA deposition in promotion chicken Pending CN110521874A (en)

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Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107087728A (en) * 2016-02-18 2017-08-25 魏全 A kind of feed addictive and preparation method
CN107981087A (en) * 2017-11-30 2018-05-04 山东和美集团有限公司 Improve feed of laying hen Egg Quality and preparation method thereof
CN112088977A (en) * 2020-09-30 2020-12-18 湖南九鼎科技（集团）有限公司 Antibiotic-free compound feed for improving lactation performance of sows

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107087728A (en) * 2016-02-18 2017-08-25 魏全 A kind of feed addictive and preparation method
CN107981087A (en) * 2017-11-30 2018-05-04 山东和美集团有限公司 Improve feed of laying hen Egg Quality and preparation method thereof
CN112088977A (en) * 2020-09-30 2020-12-18 湖南九鼎科技（集团）有限公司 Antibiotic-free compound feed for improving lactation performance of sows

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
BO YANG ET AL.: "Soybean isoflavones improve the health benefits、flavour quality indicators and physical properties of grass carp (Ctenopharygodon idella)", 《PLOS ONE》 *
MEGHAN L MANOR ET AL.: "Inclusion of Dietary Defatted Microalgae Dose-Dependently Enriches ω-3 Fatty Acids in Egg Yolk and Tissues of Laying Hens", 《THE JOURNAL OF NUTRITION》 *
路延笃: "《真核微藻逆境响应关键基因及分子调控机理研究》", 31 January 2018 *

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