CN109938044A - A kind of medicament that Wheat in Grain Filling Stage high temperature damage is effectively relieved and method - Google Patents
A kind of medicament that Wheat in Grain Filling Stage high temperature damage is effectively relieved and method Download PDFInfo
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- rhamnolipid
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Abstract
The invention discloses a kind of medicament that Wheat in Grain Filling Stage high temperature damage is effectively relieved and method, the medicament is diluted by medicament stoste, and the medicament stoste group becomes KH2PO4, Lamivudine, rhamnolipid, water, in terms of mass concentration, KH2PO4For 8~12g/L, Lamivudine be 0.2~0.4mg/L, 2~5g/L of rhamnolipid, surplus are water, and the pH value of the medicament stoste is 6.6, and the method that Wheat in Grain Filling Stage high temperature damage is effectively relieved is the following steps are included: S1, KH2PO4Medicament I is prepared, Lamivudine and rhamnolipid prepare medicament II, and medicament I and the mixing of medicament II are made into medicament stoste；S2, medicament stoste is diluted into obtain medicament；S3, wheat is sprayed using medicament.Medicament proposed by the present invention can alleviate harm of the high temperature to wheat, and spraying method is easy to be reliable, convenient for promoting the use of.
Description
Technical field
The present invention relates to regulation cereal crops late growth stage physiological processes technical field more particularly to one kind be effectively relieved it is small
The medicament and method of wheat High Temperature during Grain Filling harm.
Background technique
Wheat is one of main cereal crops in China, and the height of yield is related to the grain security in China.Wheat seed
The grain filling stage is the critical period for determining wheat ultimate output and quality, and the preference temperature in the stage is 20~24 DEG C.Research
Show that the high temperature more than 30 DEG C can cause wheat seed grouting to be interrupted, accelerate wheat aging, pustulation period shortening, yield reduction,
And high temperature can also be such that filling rate declines, and grain weight and yield reduce.It is shown according to meteorological data, in recent years Huang-Huai-Hai gas
Temperature is in rising trend, and wheat grain filling prometaphase temperature is up to 34.5 DEG C, and Climatic regionalization is paid attention to and research by more and more.
Forefathers are studies have shown that the pustulation period is the critical period of wheat growing process.Grouting mid-term high temperature significantly affects wheat
Grain weight, declines grain again, and grouting mid-term high temperature stress is conducive to improve activities of antioxidant enzymes, reduces Lipid peroxidation metabolism, and be in the milk
Injury caused by mid-term high temperature stress is irrecoverable.Yang Xiaojuan et al. research discovery High Temperature during Grain Filling reduces wheat flag leaf net photosynthesis
Rate and chlorophyll relative content reduce grain starch accumulation amount and mass of 1000 kernel.Cao Caiyun et al. research discovery grouting mid-term is high
Temperature significantly shortens grouting time, reduces kernel grouting rate.
Existing research shows that Wheat in Grain Filling Stage Climatic regionalization can be effectively relieved by spraying certain change modulator.Wheat in Grain Filling Stage
Climatic regionalization can be effectively relieved by spraying abscisic acid (ABA), improve Grain Weight in Common Wheat, especially Superiors grains grain weight；Pustulation period applies magnesium
Fertilizer is conducive to improve wheat dry-matter accumulation and transhipment amount, is effectively relieved and rear high temperature is spent to help the injury of leave photosynthetic ability
In the accumulation of photosynthate and the formation of yield, kernel grouting rate and yield are improved；Pustulation period sprays salicylic acid (SA) and can show
The wheat polyphenoils enzymatic activity promoted under High temperature stress is write, extra active oxygen is removed, to improve wheat leaf blade
To High temperature stress resistance.
KH2PO4Have the characteristics that low in cost, application is convenient, person poultry harmless, is applied multiple times in recent years raw to crops
In growth process.Zheng Xianfeng et al. research discovery Wheat in Grain Filling Stage sprays KH2PO4Be conducive to improve mass of 1000 kernel；Han Zongyou et al. research
It was found that by the Integration of Water into Fertilizer, foliage-spray KH2PO4, Resistance of Wheat To Adversity can enhance, and Biological Characteristics of Wheat becomes excellent, volume increase
The effect of increasing income is obvious；Yang Jun et al. research discovery foliage-spray KH2PO4Be conducive to improve early rice yield；Chen Tiecheng et al. research hair
Existing foliage-spray KH2PO4Be conducive to improve soybean yields and protein content.But in the prior art and have not seen related KH2PO4
Influence to Wheat in Grain Filling Stage Climatic regionalization.Based on this, the invention proposes one kind, and Wheat in Grain Filling Stage high temperature damage is effectively relieved
Medicament and method.
Summary of the invention
The purpose of the present invention is to solve disadvantages existing in the prior art, and wheat filling is effectively relieved in the one kind proposed
The medicament and method of slurry phase high temperature damage.
A kind of medicament that Wheat in Grain Filling Stage high temperature damage is effectively relieved, the medicament is diluted by medicament stoste, described
Medicament stoste group becomes KH2PO4, Lamivudine, rhamnolipid, water, in terms of mass concentration, KH2PO4For 8~12g/L, rummy husband
Being set to 0.2~0.4mg/L, 2~5g/L of rhamnolipid, surplus is water, and the pH value of the medicament stoste is 6.6.
Preferably, the medicament stoste group becomes KH2PO4, Lamivudine, rhamnolipid, water, in terms of mass concentration,
KH2PO4It is water for 10g/L, Lamivudine 0.3mg/L, rhamnolipid 4g/L, surplus.
The invention also provides a kind of methods that Wheat in Grain Filling Stage high temperature damage is effectively relieved, comprising the following steps:
S1, according to KH in medicament stoste2PO4, Lamivudine, rhamnolipid mass concentration be respectively 8~12g/L, 0.2
~0.4mg/L and 2~5g/L weigh KH2PO4, Lamivudine and rhamnolipid, and by weighed KH2PO4With suitable water-soluble
Solution, obtains medicament I, will weigh and suitable water is added in Lamivudine and rhamnolipid in 40~50 DEG C of 1~2h of mechanical stirring, cooling
Medicament II is obtained to room temperature, then medicament I and medicament II are mixed, adds water, constant volume is configured to KH2PO4Mass concentration be 8~12g/L,
Lamivudine is the medicament stoste of 0.2~0.4mg/L, 2~5g/L of rhamnolipid, spare；
S2, before spraying, medicament stoste prepared by step S1 is diluted with water 5 times, is configured to KH2PO4Final concentration of 1.6~
The medicament of 2.4g/L；
S3, the medicament for obtaining step S2 carry out spraying operation to wheat canopy, and after in the afternoon 5 points of spraying operation
It carries out, the time of each spraying operation is two days, and the plot of daily every 666.7 ㎡ sprays medicament 30L, if spraying behaviour above-mentioned
It rains in 6 hours after work, then next day sprays again.
Preferably, in step S1, churned mechanically temperature is 46 DEG C in water for Lamivudine and rhamnolipid, mechanical stirring
Time be 1.5h, be cooled to room temperature to obtain medicament II.
Preferably, in step S3, time of the spraying operation be high-temperature process before, high-temperature process when, after high-temperature process
One of.
Preferably, before the high-temperature process for the wheat grain filling rapid increase phase a few days ago, the high-temperature process Shi Wei little
Wheat is in the milk the centre two days of rapid increase phase, after the high-temperature process for the wheat grain filling rapid increase phase after two days.
The present invention has the effect that
1, Wheat Photosynthesis rate can be improved by spraying medicament to wheat canopy, promote the generation of chlorophyll, improve photosynthesis
Dark reaction, Delaying Leaf-Senescence, and can reduce plant organ-tissue damage, the ability for making photoassimilates be sent to fringe and grain
It is promoted, reduces mda content, improve activities of antioxidant enzymes；Extend grouting time, increases wheat grain filling peak value, make seed more
Full, yield is promoted；
2, the KH that the present invention passes through reasonable concentration2PO4, the medicament stoste that is formulated of Lamivudine, rhamnolipid and water,
Convenient for storage and transport, stability is good, easy to use, and using preceding dilution, and the medicament after dilution can be wheat growth
Sufficient nutrient, especially KH are provided2PO4Suitable phosphate fertilizer and potash fertilizer can be provided for wheat, guarantee the normal life of wheat
It is long, and cooperate medicament II made of Lamivudine and rhamnolipid compounding, wheat can be significantly improved to the tolerance of high temperature, and
The experiment proved that spraying medicament within continuous two days after high temperature generation, it can significantly alleviate harm of the high temperature to wheat.
3, the raw material that the present invention uses, abundance is cheap, and medicament is easily prepared, and spraying method is easy to be reliable, people and animals
It is harmless, convenient for promoting the use of.
Specific embodiment
Combined with specific embodiments below the present invention is made further to explain.
Embodiment
A kind of medicament that Wheat in Grain Filling Stage high temperature damage is effectively relieved proposed by the present invention, the medicament are dilute by medicament stoste
It releases, the medicament stoste group becomes KH2PO4, Lamivudine, rhamnolipid, water, in terms of mass concentration, KH2PO4For 10g/
L, Lamivudine is 0.3mg/L, rhamnolipid 4g/L, surplus are water, and the pH value of the medicament stoste is 6.6.
The invention also provides a kind of methods that Wheat in Grain Filling Stage high temperature damage is effectively relieved, comprising the following steps:
S1, wheat planting:
Two experimental plots, respectively the first experimental plot and the second experimental plot are set up, and plant Huaihe River wheat 33 in the first experimental plot,
Second experimental plot plantation peace agriculture 0711, was sowed, application rate: the first 10.92 kgs/acre of experimental plot, second on November 5th, 2017
9.63 kgs/acre of experimental plot carries out wheat weighing, broadcast application, ploughs, rakes, manually ditching, sowing, suppression before sowing, fertilising is pressed
According to the form fertilising of 5:5,666.7 ㎡ basal dressings pure N 7.5Kg, K2O 7.5Kg, P2O5 7.5Kg, next year, in wheat during jointing stage
Urea 7.5Kg is imposed, remaining same Routine Management；
The preparation of S2, medicament stoste:
Weigh KH2PO410g adds suitable water to dissolve, and obtains medicament I, weighs Lamivudine 0.3mg, rhamnolipid 4g, add suitable
The water of amount mechanical stirring 1.5h at 46 DEG C is cooled to room temperature to obtain medicament II, then medicament I and medicament II is mixed, and adds water, fixed
Hold, is configured to KH2PO4Mass concentration is 10g/L, the medicament stoste of Lamivudine 0.3mg/L, rhamnolipid 4g/L, the medicine
The pH value of agent stoste is 6.6；
The preparation of S3, medicament:
Before spraying, medicament stoste prepared by step S2 is diluted with water 5 times, is configured to KH2PO4The medicine of final concentration of 2g/L
Agent；
S4, crop field spray:
First experimental plot is divided into 7 parts, i.e. 1-1,1-2,1-3,1-4,1-5,1-6,1-7, the second experimental plot is divided equally
For 7 parts, i.e. 2-1,2-2,2-3,2-4,2-5,2-6,2-7, and the blank control group that 1-1 and 2-1 is not handled as high temperature, it will
1-2 and 2-2 sprays clear water as the control group for spraying clear water before high-temperature process for 2 days, using 1-3 and 2-3 as 2 days when high-temperature process
Control group, using 1-4 and 2-4 as the control group for spraying clear water after high-temperature process for 2 days, using 1-5 and 2-5 as high-temperature process before
The test group for spraying medicament for 2 days makees 1-7 and 2-7 using 1-6 and 2-6 as the test group for spraying medicament when high-temperature process for 2 days
For the test group for spraying medicament after high-temperature process for 2 days, and before high-temperature process the time of spraying operation be on April 24th, 2018 and
On April 25th, 2018, the time of spraying operation is on April 27th, 2018 and on April 28th, 2018, high-temperature process when high-temperature process
The time of spraying operation is on May 4th, 2018 and on May 5th, 2018 afterwards.Before high-temperature process, when high-temperature process and high-temperature process
It is carried out after in the afternoon 5 points of spraying operation afterwards, is uniformly sprayed on wheat strain, the daily plot every 666.7 ㎡ sprays medicament
30L, if raining in 6 hours after above-mentioned spraying operation, next day sprays again.
Progress on May 27th, 2018 harvesting wheat carries out species test after harvest and surveys to produce, the results are shown in Table 1.
May 6 was sampled (boot leaf) test group that medicament is sprayed after high-temperature process, places -80 DEG C of refrigerator low temperature and protects
It deposits, carries out MDA, SOD, POD, CAT determination of activity in August, 2018, the results are shown in Table 2.
Table 1: influence of the medicament to 0711 yield of Huaihe River wheat 33 and An Nong is sprayed
From 1 experimental result of table: compared to high temperature not processing operation, carrying out spraying clear water before, during and after high-temperature process
All be conducive to boot leaf grain number per spike, mass of 1000 kernel and yield with medicament is sprayed, and spray medicament compared to the width for spraying clear water promotion
Degree is more significant, and the effect for spraying medicament for 2 days especially after high-temperature process is best, in addition to this, it has been found that sprays medicament pair
The treatment effect of Huaihe River wheat 33 and An Nong 0711 have identical rule, show: medicament proposed by the present invention and method can be effective
Alleviate harm of the high temperature to wheat.
Table 2: influence of the medicament to Huaihe River wheat 33 and An Nong 0711 photosynthetic rate and anti-oxidant enzyme activity is sprayed within 2 days after high temperature
From 2 experimental result of table: it sprays within 2 days after high temperature medicament of the invention and is conducive to improve boot leaf Net Photosynthetic Rate,
Activities of antioxidant enzymes is improved, reduces Lipid peroxidation metabolism, and Huaihe River wheat 33 and An Nong 0711 have identical rule.
The foregoing is only a preferred embodiment of the present invention, but scope of protection of the present invention is not limited thereto,
Anyone skilled in the art in the technical scope disclosed by the present invention, according to the technique and scheme of the present invention and its
Inventive concept is subject to equivalent substitution or change, should be covered by the protection scope of the present invention.
Claims (6)
1. a kind of medicament that Wheat in Grain Filling Stage high temperature damage is effectively relieved, which is characterized in that the medicament is diluted by medicament stoste
It forms, the medicament stoste group becomes KH2PO4, Lamivudine, rhamnolipid, water, in terms of mass concentration, KH2PO4For 8~
12g/L, Lamivudine are 0.2~0.4mg/L, 2~5g/L of rhamnolipid, surplus are water, and the pH value of the medicament stoste is
6.6。
2. a kind of medicament that Wheat in Grain Filling Stage high temperature damage is effectively relieved according to claim 1, which is characterized in that described
Medicament stoste group becomes KH2PO4, Lamivudine, rhamnolipid, water, in terms of mass concentration, KH2PO4For 10g/L, Lamivudine
It is water for 0.3mg/L, rhamnolipid 4g/L, surplus.
3. a kind of method that Wheat in Grain Filling Stage high temperature damage is effectively relieved, which comprises the following steps:
S1, according to KH in medicament stoste2PO4, Lamivudine, rhamnolipid mass concentration be respectively 8~12g/L, 0.2~
0.4mg/L and 2~5g/L weigh KH2PO4, Lamivudine and rhamnolipid, and by weighed KH2PO4It is dissolved with suitable water,
Medicament I is obtained, will weigh and suitable water is added in Lamivudine and rhamnolipid in 40~50 DEG C of 1~2h of mechanical stirring, be cooled to
Room temperature obtains medicament II, then medicament I and medicament II are mixed, and adds water, constant volume is configured to KH2PO4Mass concentration is 8~12g/L, drawing
Rice husband is set to the medicament stoste of 0.2~0.4mg/L, 2~5g/L of rhamnolipid, spare；
S2, before spraying, medicament stoste prepared by step S1 is diluted with water 5 times, is configured to KH2PO4Final concentration of 1.6~2.4g/
The medicament of L；
S3, the medicament for obtaining step S2 carry out spraying operation to wheat canopy, and in the afternoon 5 points of spraying operation with laggard
Row, the time of each spraying operation is two days, and the plot of daily every 666.7 ㎡ sprays medicament 30L, if in above-mentioned spraying operation
It rains in 6 hours afterwards, then next day sprays again.
4. a kind of method that Wheat in Grain Filling Stage high temperature damage is effectively relieved according to claim 3, which is characterized in that step
In S1, churned mechanically temperature is 46 DEG C in water for Lamivudine and rhamnolipid, and the churned mechanically time is 1.5h, is cooled to
Room temperature obtains medicament II.
5. a kind of method that Wheat in Grain Filling Stage high temperature damage is effectively relieved according to claim 3, which is characterized in that step
In S3, time of the spraying operation be high-temperature process before, high-temperature process when, one of after high-temperature process.
6. a kind of method that Wheat in Grain Filling Stage high temperature damage is effectively relieved according to claim 4, which is characterized in that described
Before high-temperature process a few days ago for the wheat grain filling rapid increase phase, in the wheat grain filling rapid increase phase when high-temperature process
Between two days, after the high-temperature process for the wheat grain filling rapid increase phase after two days.
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