CN109880400A - A kind of extraction process of the anti-oxidant uranidin of Snakegourd Fruit - Google Patents
A kind of extraction process of the anti-oxidant uranidin of Snakegourd Fruit Download PDFInfo
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- CN109880400A CN109880400A CN201910226020.3A CN201910226020A CN109880400A CN 109880400 A CN109880400 A CN 109880400A CN 201910226020 A CN201910226020 A CN 201910226020A CN 109880400 A CN109880400 A CN 109880400A
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Abstract
The present invention provides a kind of extraction process of anti-oxidant uranidin of Snakegourd Fruit, is related to uranidin extractive technique field.The extraction process of the anti-oxidant uranidin mainly has: slice cook, dries pulverizing, heat preservation enzymatic hydrolysis, freezing melt, centrifugation mixing, filtering absorption, ultrafiltration freeze-drying and etc..The present invention overcomes the deficiencies in the prior art, the pericarp and pulp for efficiently using Snakegourd Fruit carry out uranidin extraction, increase the economic benefit of Snakegourd Fruit, and extracting method is safe and efficient, resulting uranidin is made to have many advantages, such as that purity is high, stability is good, antioxidant effect is strong.
Description
Technical field
The present invention relates to uranidin extractive technique fields, and in particular to a kind of extraction process of the anti-oxidant uranidin of Snakegourd Fruit.
Background technique
The state that China is also in synthetic dyestuff and natural pigment at present and deposits and develop simultaneously, due to the peace of synthetic dyestuff
Full property problem, synthetic dyestuff China allow using degree limited significantly, especially in food, medicine and cosmetic industry,
Therefore the application of natural pigment is the Main way of future development.
Snakegourd Fruit be it is a kind of have the effects that it is antipyretic quench the thirst, diuresis, kobadrin plant, wherein seed containing rouge flower fat oil,
Fruit contains triterpenoid saponin, organic acid, resin, carbohydrate, pigment, and root contains protein, saponin(e, acids.Large area plantation at this stage
Snakegourd Fruit is mostly to be extracted oil using its seed, and PERICARPIUM TRICHOSANTHIS and pulp often result in waste.How these ingredients progress yellow is utilized
The extraction of element makes a research direction at this stage.
Summary of the invention
In view of the shortcomings of the prior art, the present invention provides a kind of extraction process of anti-oxidant uranidin of Snakegourd Fruit, melon is efficiently used
The pericarp and pulp of beach wormwood carry out uranidin extraction, increase the economic benefit of Snakegourd Fruit, and extracting method is safe and efficient, make resulting
Uranidin has many advantages, such as that purity is high, stability is good, antioxidant effect is strong.
In order to achieve the above object, technical solution of the present invention is achieved by the following technical programs:
A kind of extraction process of the anti-oxidant uranidin of Snakegourd Fruit, the extraction process of the anti-oxidant uranidin includes following step
It is rapid:
(1) it will be cleaned up after mature Snakegourd Fruit deseeding, after drying surface moisture, be cut into sheet, be laid in food steamer, water proof
A period of time is steamed, it is spare to obtain ripe Snakegourd Fruit;
(2) above-mentioned ripe Snakegourd Fruit is placed in baking oven progress high temperature drying, after pulverization process is carried out in pulverizer, obtain Snakegourd Fruit
It is spare after powder sieving;
(3) deionized water is added after cellulase being added in above-mentioned Snakegourd Fruit powder, after being mixed evenly, water-bath keeps the temperature enzyme
Solution a period of time, it is spare to obtain mixed liquor;
(4) it is rapidly frozen solidification a period of time after above-mentioned mixed liquor being carried out ultrasonic vibration processing, room temperature melts after taking-up,
Carry out high speed centrifugation in centrifuge, sediment be added after ethanol solution keep the temperature standing a period of time after mixing evenly continue from
The heart merges the clear liquid being centrifuged twice, it is spare to obtain solution A;
(5) above-mentioned solution A is passed through into microfiltration membranes, it is spare obtains solution B, then solution B is subjected to macroporous absorbent resin absorption, and
With 1.5-2 times of amount of resin of ethanol elution, it is spare to obtain eluent;
(6) eluent is subjected to ethyl alcohol recycling, then carries out high temperature concentrated by rotary evaporation, be finally spray-dried, obtain yellow
Plain crude product is spare;
(7) by ultrafiltration membrane after above-mentioned crude yellow element addition deionized water being mixed evenly, efflux is obtained, then will
Efflux carries out low temperature freeze-drying, obtains anti-oxidant uranidin of the invention.
Preferably, the steam temperature of Snakegourd Fruit Steam by water bath is 100-110 DEG C in the step (1), time 5-8min.
Preferably, the drying temperature of baking oven is 70-80 DEG C in the step (2), water content≤5% of Snakegourd Fruit after drying,
And Snakegourd Fruit powder crosses 40-60 mesh.
Preferably, the mass ratio of Snakegourd Fruit powder and cellulase, deionized water is 3: 0.01: 20 in the step (3), and bath is protected
The temperature of temperature enzymatic hydrolysis is 45-55 DEG C, enzymolysis time 30-40min.
Preferably, the frequency of ultrasonic vibration is 40KHz in the step (4), and power 220W, the concussion time is 15-
20min, the temperature of cryogenic freezing is subzero 5 DEG C-it is 10 DEG C, cooling time 15-20min subzero, and centrifuge is ultracentrifugal
Revolving speed is 4200r/min, centrifugation time 10-12min, and it is 80-85 DEG C that the temperature that ethyl alcohol heat preservation is stood, which is added, and time of repose is
30-40min。
Preferably, the filter opening diameter of microfiltration membranes is 0.1-0.5 μm in the step (5).
Preferably, the temperature of step (6) the high temperature revolving is 100-120 DEG C, and volume is original volume after concentration
1/3.
Preferably, ultrafiltration membrane used in the step (7) is the PS membrane that molecular cut off is 10000.
The present invention provides a kind of extraction process of anti-oxidant uranidin of Snakegourd Fruit, and advantage is compared with prior art:
(1) present invention, which uses Snakegourd Fruit deseeding to dry after boiling and grinds resulting Snakegourd Fruit powder, extracts uranidin for raw material,
The other positions efficiently used outside Snakegourd Fruit oil expression generate economic benefit, and Snakegourd Fruit can be further reduced the later period after boiling
The impurity of finished product promotes the purity of product, reduces influence of the lubricant component to product.
(2) present invention addition cellulase handles Snakegourd Fruit, effectively removes the cellulose in Snakegourd Fruit, mentions final products
Purity, and use ultrasonic vibration and Refrigeration Technique, effectively extract the uranidin in Snakegourd Fruit, and have resulting uranidin
There is higher inoxidizability, promotes the quality of product.
(3) present invention gained uranidin is free of any chemical addition agent, safety and environmental protection, and will extract crude product through filter membrane
And adsorption treatment, so that product colour is pure, quality is good, with high purity, and stability with higher.
Specific embodiment
In order to make the object, technical scheme and advantages of the embodiment of the invention clearer, below with reference to the embodiment of the present invention pair
Technical solution in the embodiment of the present invention is clearly and completely described, it is clear that described embodiment is a part of the invention
Embodiment, instead of all the embodiments.Based on the embodiments of the present invention, those of ordinary skill in the art are not making wound
Every other embodiment obtained under the premise of the property made labour, shall fall within the protection scope of the present invention.
Embodiment 1:
A kind of extraction process of the anti-oxidant uranidin of Snakegourd Fruit, the extraction process of the anti-oxidant uranidin includes following step
It is rapid:
(1) it will be cleaned up after mature Snakegourd Fruit deseeding, after drying surface moisture, be cut into sheet, be laid in food steamer, water proof
A period of time is steamed, it is spare to obtain ripe Snakegourd Fruit;
(2) above-mentioned ripe Snakegourd Fruit is placed in baking oven progress high temperature drying, after pulverization process is carried out in pulverizer, obtain Snakegourd Fruit
It is spare after powder sieving;
(3) deionized water is added after cellulase being added in above-mentioned Snakegourd Fruit powder, after being mixed evenly, water-bath keeps the temperature enzyme
Solution a period of time, it is spare to obtain mixed liquor;
(4) it is rapidly frozen solidification a period of time after above-mentioned mixed liquor being carried out ultrasonic vibration processing, room temperature melts after taking-up,
Carry out high speed centrifugation in centrifuge, sediment be added after ethanol solution keep the temperature standing a period of time after mixing evenly continue from
The heart merges the clear liquid being centrifuged twice, it is spare to obtain solution A;
(5) above-mentioned solution A is passed through into microfiltration membranes, it is spare obtains solution B, then solution B is subjected to macroporous absorbent resin absorption, and
With 1.5-2 times of amount of resin of ethanol elution, it is spare to obtain eluent;
(6) eluent is subjected to ethyl alcohol recycling, then carries out high temperature concentrated by rotary evaporation, be finally spray-dried, obtain yellow
Plain crude product is spare;
(7) by ultrafiltration membrane after above-mentioned crude yellow element addition deionized water being mixed evenly, efflux is obtained, then will
Efflux carries out low temperature freeze-drying, obtains anti-oxidant uranidin of the invention.
Wherein, the steam temperature of Snakegourd Fruit Steam by water bath is 100 DEG C in the step (1), time 5min;The step
(2) drying temperature of baking oven is 70 DEG C in, water content≤5% of Snakegourd Fruit after drying, and Snakegourd Fruit powder crosses 40 meshes;The step
(3) mass ratio of Snakegourd Fruit powder and cellulase, deionized water is 3: 0.01: 20 in, and the temperature of bath heat preservation enzymatic hydrolysis is 45 DEG C, enzymatic hydrolysis
Time is 30min;The frequency of ultrasonic vibration is 40KHz in the step (4), and power 220W, the concussion time is 15min, low
The temperature of temperature freezing is subzero 5 DEG C, cooling time 15min, and the ultracentrifugal revolving speed of centrifuge is 4200r/min, centrifugation
Time is 10min, and it is 80-85 DEG C that the temperature that ethyl alcohol heat preservation is stood, which is added, time of repose 30min;Micro-filtration in the step (5)
The filter opening diameter of film is 0.1-0.5 μm;The temperature of step (6) the high temperature revolving is 100 DEG C, and volume is original body after concentration
Long-pending 1/3;Ultrafiltration membrane used in the step (7) is the PS membrane that molecular cut off is 10000.
Embodiment 2:
A kind of extraction process of the anti-oxidant uranidin of Snakegourd Fruit, the extraction process of the anti-oxidant uranidin includes following step
It is rapid:
(1) it will be cleaned up after mature Snakegourd Fruit deseeding, after drying surface moisture, be cut into sheet, be laid in food steamer, water proof
A period of time is steamed, it is spare to obtain ripe Snakegourd Fruit;
(2) above-mentioned ripe Snakegourd Fruit is placed in baking oven progress high temperature drying, after pulverization process is carried out in pulverizer, obtain Snakegourd Fruit
It is spare after powder sieving;
(3) deionized water is added after cellulase being added in above-mentioned Snakegourd Fruit powder, after being mixed evenly, water-bath keeps the temperature enzyme
Solution a period of time, it is spare to obtain mixed liquor;
(4) it is rapidly frozen solidification a period of time after above-mentioned mixed liquor being carried out ultrasonic vibration processing, room temperature melts after taking-up,
Carry out high speed centrifugation in centrifuge, sediment be added after ethanol solution keep the temperature standing a period of time after mixing evenly continue from
The heart merges the clear liquid being centrifuged twice, it is spare to obtain solution A;
(5) above-mentioned solution A is passed through into microfiltration membranes, it is spare obtains solution B, then solution B is subjected to macroporous absorbent resin absorption, and
With 1.5-2 times of amount of resin of ethanol elution, it is spare to obtain eluent;
(6) eluent is subjected to ethyl alcohol recycling, then carries out high temperature concentrated by rotary evaporation, be finally spray-dried, obtain yellow
Plain crude product is spare;
(7) by ultrafiltration membrane after above-mentioned crude yellow element addition deionized water being mixed evenly, efflux is obtained, then will
Efflux carries out low temperature freeze-drying, obtains anti-oxidant uranidin of the invention.
Wherein, the steam temperature of Snakegourd Fruit Steam by water bath is 110 DEG C in the step (1), time 8min;The step
(2) drying temperature of baking oven is 80 DEG C in, water content≤5% of Snakegourd Fruit after drying, and Snakegourd Fruit powder crosses 60 meshes;The step
(3) mass ratio of Snakegourd Fruit powder and cellulase, deionized water is 3: 0.01: 20 in, and the temperature of bath heat preservation enzymatic hydrolysis is 55 DEG C, enzymatic hydrolysis
Time is 40min;The frequency of ultrasonic vibration is 40KHz in the step (4), and power 220W, the concussion time is 20min, low
The temperature of temperature freezing is subzero 10 DEG C, cooling time 20min, and the ultracentrifugal revolving speed of centrifuge is 4200r/min, centrifugation
Time is 10-12min, and it is 80-85 DEG C that the temperature that ethyl alcohol heat preservation is stood, which is added, time of repose 40min;In the step (5)
The filter opening diameter of microfiltration membranes is 0.1-0.5 μm;The temperature of step (6) the high temperature revolving is 120 DEG C, and volume is original after concentration
There is the 1/3 of volume;Ultrafiltration membrane used in the step (7) is the PS membrane that molecular cut off is 10000.
Embodiment 3:
A kind of extraction process of the anti-oxidant uranidin of Snakegourd Fruit, the extraction process of the anti-oxidant uranidin includes following step
It is rapid:
(1) it will be cleaned up after mature Snakegourd Fruit deseeding, after drying surface moisture, be cut into sheet, be laid in food steamer, water proof
A period of time is steamed, it is spare to obtain ripe Snakegourd Fruit;
(2) above-mentioned ripe Snakegourd Fruit is placed in baking oven progress high temperature drying, after pulverization process is carried out in pulverizer, obtain Snakegourd Fruit
It is spare after powder sieving;
(3) deionized water is added after cellulase being added in above-mentioned Snakegourd Fruit powder, after being mixed evenly, water-bath keeps the temperature enzyme
Solution a period of time, it is spare to obtain mixed liquor;
(4) it is rapidly frozen solidification a period of time after above-mentioned mixed liquor being carried out ultrasonic vibration processing, room temperature melts after taking-up,
Carry out high speed centrifugation in centrifuge, sediment be added after ethanol solution keep the temperature standing a period of time after mixing evenly continue from
The heart merges the clear liquid being centrifuged twice, it is spare to obtain solution A;
(5) above-mentioned solution A is passed through into microfiltration membranes, it is spare obtains solution B, then solution B is subjected to macroporous absorbent resin absorption, and
With 1.5-2 times of amount of resin of ethanol elution, it is spare to obtain eluent;
(6) eluent is subjected to ethyl alcohol recycling, then carries out high temperature concentrated by rotary evaporation, be finally spray-dried, obtain yellow
Plain crude product is spare;
(7) by ultrafiltration membrane after above-mentioned crude yellow element addition deionized water being mixed evenly, efflux is obtained, then will
Efflux carries out low temperature freeze-drying, obtains anti-oxidant uranidin of the invention.
Wherein, the steam temperature of Snakegourd Fruit Steam by water bath is 105 DEG C in the step (1), time 7min;The step
(2) drying temperature of baking oven is 75 DEG C in, water content≤5% of Snakegourd Fruit after drying, and Snakegourd Fruit powder crosses 50 meshes;The step
(3) mass ratio of Snakegourd Fruit powder and cellulase, deionized water is 3: 0.01: 20 in, and the temperature of bath heat preservation enzymatic hydrolysis is 50 DEG C, enzymatic hydrolysis
Time is 35min;The frequency of ultrasonic vibration is 40KHz in the step (4), and power 220W, the concussion time is 15-20min,
The temperature of cryogenic freezing is subzero 8 DEG C, cooling time 18min, and the ultracentrifugal revolving speed of centrifuge is 4200r/min, from
The heart time is 10-12min, and it is 80-85 DEG C that the temperature that ethyl alcohol heat preservation is stood, which is added, time of repose 35min;The step (5)
The filter opening diameter of middle microfiltration membranes is 0.1-0.5 μm;The temperature of step (6) the high temperature revolving is 110 DEG C, and volume is after concentration
The 1/3 of original volume;Ultrafiltration membrane used in the step (7) is the PS membrane that molecular cut off is 10000.
It should be noted that, in this document, relational terms such as first and second and the like are used merely to a reality
Body or operation are distinguished with another entity or operation, are deposited without necessarily requiring or implying between these entities or operation
In any actual relationship or order or sequence.Moreover, the terms "include", "comprise" or its any other variant are intended to
Non-exclusive inclusion, so that the process, method, article or equipment including a series of elements is not only wanted including those
Element, but also including other elements that are not explicitly listed, or further include for this process, method, article or equipment
Intrinsic element.In the absence of more restrictions, the element limited by sentence "including a ...", it is not excluded that
There is also other identical elements in process, method, article or equipment including the element.
The above embodiments are merely illustrative of the technical solutions of the present invention, rather than its limitations;Although with reference to the foregoing embodiments
Invention is explained in detail, those skilled in the art should understand that: it still can be to aforementioned each implementation
Technical solution documented by example is modified or equivalent replacement of some of the technical features;And these modification or
Replacement, the spirit and scope for technical solution of various embodiments of the present invention that it does not separate the essence of the corresponding technical solution.
Claims (8)
1. a kind of extraction process of the anti-oxidant uranidin of Snakegourd Fruit, which is characterized in that the extraction process packet of the anti-oxidant uranidin
Include following steps:
(1) it will be cleaned up after mature Snakegourd Fruit deseeding, after drying surface moisture, be cut into sheet, be laid in food steamer, Steam by water bath one
The section time, it is spare to obtain ripe Snakegourd Fruit;
(2) above-mentioned ripe Snakegourd Fruit is placed in baking oven progress high temperature drying, after pulverization process is carried out in pulverizer, obtain Snakegourd Fruit powder mistake
It is spare after sieve;
(3) deionized water is added after cellulase being added in above-mentioned Snakegourd Fruit powder, after being mixed evenly, water-bath heat preservation enzymatic hydrolysis one
The section time, it is spare to obtain mixed liquor;
(4) it is rapidly frozen solidification a period of time after above-mentioned mixed liquor being carried out ultrasonic vibration processing, room temperature melts after taking-up, then at
High speed centrifugation is carried out in centrifuge, sediment, which is added after ethanol solution keeps the temperature standing a period of time after mixing evenly, to be continued to be centrifuged,
Merge the clear liquid being centrifuged twice, it is spare to obtain solution A;
(5) above-mentioned solution A is passed through into microfiltration membranes, it is spare to obtain solution B, then solution B is carried out macroporous absorbent resin absorption, and with setting
The ethanol elution of 1.5-2 times of rouge amount, it is spare to obtain eluent;
(6) eluent is subjected to ethyl alcohol recycling, then carries out high temperature concentrated by rotary evaporation, be finally spray-dried, it is thick to obtain uranidin
Product are spare;
(7) by ultrafiltration membrane after above-mentioned crude yellow element addition deionized water being mixed evenly, efflux is obtained, then will outflow
Liquid carries out low temperature freeze-drying, obtains anti-oxidant uranidin of the invention.
2. a kind of extraction process of the anti-oxidant uranidin of Snakegourd Fruit according to claim 1, it is characterised in that: the step
(1) steam temperature of Snakegourd Fruit Steam by water bath is 100-110 DEG C in, time 5-8min.
3. a kind of extraction process of the anti-oxidant uranidin of Snakegourd Fruit according to claim 1, it is characterised in that: the step
(2) drying temperature of baking oven is 70-80 DEG C in, water content≤5% of Snakegourd Fruit after drying, and Snakegourd Fruit powder crosses 40-60 mesh.
4. a kind of extraction process of the anti-oxidant uranidin of Snakegourd Fruit according to claim 1, it is characterised in that: the step
(3) mass ratio of Snakegourd Fruit powder and cellulase, deionized water is 3: 0.01: 20 in, and the temperature of bath heat preservation enzymatic hydrolysis is 45-55 DEG C,
Enzymolysis time is 30-40min.
5. a kind of extraction process of the anti-oxidant uranidin of Snakegourd Fruit according to claim 1, it is characterised in that: the step
(4) frequency of ultrasonic vibration is 40KHz in, and power 220W, the concussion time is 15-20min, and the temperature of cryogenic freezing is subzero
5 DEG C -10 DEG C, cooling time 15-20min subzero, and the ultracentrifugal revolving speed of centrifuge is 4200r/min, centrifugation time is
10-12min, it is 80-85 DEG C that the temperature that ethyl alcohol heat preservation is stood, which is added, time of repose 30-40min.
6. a kind of extraction process of the anti-oxidant uranidin of Snakegourd Fruit according to claim 1, it is characterised in that: the step
(5) the filter opening diameter of microfiltration membranes is 0.1-0.5 μm in.
7. a kind of extraction process of the anti-oxidant uranidin of Snakegourd Fruit according to claim 1, it is characterised in that: the step
(6) temperature of high temperature revolving is 100-120 DEG C, and volume is the 1/3 of original volume after concentration.
8. a kind of extraction process of the anti-oxidant uranidin of Snakegourd Fruit according to claim 1, it is characterised in that: the step
(7) ultrafiltration membrane used in is the PS membrane that molecular cut off is 10000.
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Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
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CN103087548A (en) * | 2013-01-29 | 2013-05-08 | 安阳天尊生物工程有限公司 | Method for extracting trichosanthes kirilowii maxim uranidin |
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Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
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CN103087548A (en) * | 2013-01-29 | 2013-05-08 | 安阳天尊生物工程有限公司 | Method for extracting trichosanthes kirilowii maxim uranidin |
Non-Patent Citations (3)
Title |
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全若琳 等: "超声辅助提取栝楼皮黄色素工艺优化", 《吉首大学学报(自然科学版)》 * |
孙体健 等: "栝楼黄色素的提取及性能研究", 《中国食品卫生杂志》 * |
房照龙 等: "现代提取技术在天然色素领域的应用", 《广州化工》 * |
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Application publication date: 20190614 |