CN109846939A - The fermented tcm extracting solution for preventing and treating piglet bacillary dysentery - Google Patents
The fermented tcm extracting solution for preventing and treating piglet bacillary dysentery Download PDFInfo
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- CN109846939A CN109846939A CN201910080059.9A CN201910080059A CN109846939A CN 109846939 A CN109846939 A CN 109846939A CN 201910080059 A CN201910080059 A CN 201910080059A CN 109846939 A CN109846939 A CN 109846939A
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Abstract
The invention belongs to contain the technical field of medicine of the plant from Angiospermae, and in particular to a kind of fermented tcm extracting solution for preventing and treating piglet bacillary dysentery.The fermented tcm extracting solution of the prevention and treatment piglet bacillary dysentery, is dried by pine needle, folium cortex eucommiae, Semen Cuscutae and the fruit of glossy privet, is crushed, is sieved, decocted and to obtain extracting solution, then obtaining through microbial bacterial agent anaerobic fermentation.Fermented tcm extracting solution of the invention is good to the control efficiency of piglet white scour；Has the function of disease-resistant growth-promoting to growing pigs；It can be improved feed conversion rate, improve daily gain；It can be improved pigling immunity；It is easy to use, it can reduce the irritability of livestock and poultry；It has no toxic side effect, securely and reliably.
Description
Technical field
The invention belongs to contain the technical field of medicine of the plant from Angiospermae, and in particular to Yi Zhongfang
Control the fermented tcm extracting solution of piglet bacillary dysentery.
Background technique
Diarrhea be piglet it is most commonly seen and influence survival rate disease of digestive tract, often become the primary cause of the death.Pig house heat preservation
Bad, sanitary condition is poor, and sow teat is unclean, and nutrition caused by subalimentation or microelement, vitamin are short of in feed is not
Entirely, abrupt change of climate (sometimes hot and sometimes cold), pathogenic infection microorganism or helminth etc. can cause piglet white scour (" piglet white scour examined
Disconnected main points and control principle ", Zhang Ming, cultural technique consultant, the 1st phase in 2010, the 68-69 pages, publication date May 10 in 2010
Day).These years, piglet white scour is extremely widespread in scale pig raising.Have data to show: piglet 30kg piglet white scour below is averagely sent out
For sick rate 46.5%, the death rate is up to 10.3%, brings great economic loss to pig breeding industry.How better prevention and control piglet
Diarrhea improves piglet survival ratio, become particularly important project in pig raising practice (" clinical diagnosis and treatment of piglet white scour ",
Liu Xingyuan, Chinese animal and veterinary digest, the 3rd phase of volume 32 in 2016, page 201, publication date on December 31st, 2016).
Currently, piglet white scour generally utilizes antibiotic treatment, inhibiting or killing to cause a disease during these antibiotic usages
While bacterium, normal flora in body is also affected, makes normal enterobacteriaceae disequilibrium, leads to flora imbalance, influence body
Normal physiological function, to seriously affect the digestive function of piglet.Secondly, bacterium is also easy to produce drug resistance and is difficult to cure, sometimes
With several medicines all invalid (" progress of antibiotic-associated diarrhea traditional Chinese medicine prevention strategy ", Zhe etc., modern Chinese medicine
Medicine, the 3rd phase of volume 26 in 2006, the 66-68 pages, publication date on December 31st, 2006；" bromogeramine treats piglet white scour effect
Well ", Zhu Xianlong, Guangxi animal and veterinary, the 3rd phase in 1991, page 33, publication date on December 31st, 1991).In addition global model
In enclosing, due to the abuse of antibiotic, this disease is caused also in worsening, to endanger even more serious.
And the pharmacological property of Chinese herbal medicine is mild, more metenteron mucous membrane smaller to internal organs damage and the problem of medicament residue
Injury recovery faster.Therefore, Chinese herbal medicine has biggish advantage (" herbal control weanling pig to the treatment of piglet white scour
The test of diarrhea ", Li Kaicai, animal husbandry, the 9th phase in 2011, the 74-75 pages, publication date on December 31st, 2011).So opening
The Chinese medicine of hair treatment piglet white scour improves China for solving the indeterminable medicament residue of chemical synthetic drug and pollution problem
Animal husbandry production level and product quality, make China's animal products can smoothly enter into international market, take part in international competition with great
Research significance.
Currently, people have carried out correlative study for the Chinese medicine for the treatment of piglet white scour.However, these drugs are under piglet
The curative effect of dysentery is slower, and dosage is big, higher cost.
Summary of the invention
In view of this, the purpose of the present invention is to provide a kind of fermented tcm extracting solution for preventing and treating piglet bacillary dysentery,
The Chinese medicine extract is to the significant in efficacy of piglet bacillary dysentery.
To achieve the above object, the technical solution of the present invention is as follows:
The fermented tcm extracting solution for preventing and treating piglet bacillary dysentery, dried by pine needle, folium cortex eucommiae, Semen Cuscutae and the fruit of glossy privet,
Extracting solution is crushed, is sieved, decocting and to obtain, is then obtained through microbial bacterial agent anaerobic fermentation.
Inventor has found in the course of the research, by the drying of pine needle, folium cortex eucommiae, Semen Cuscutae and the fruit of glossy privet, crushing, sieving, decocts
Extracting solution is boiled to obtain, then through prevention and treatment of the fermented tcm extracting solution to piglet bacillary dysentery obtained by microbial bacterial agent anaerobic fermentation
Effect is good, especially significant to the therapeutic effect of piglet yellow scour, dysentery characterized by white mucous stool.
The bacillary dysentery refers to piglet white scour caused by bacterium infection, including the piglet as caused by salmonella infection
Paratyphoid, the white scour of piglet as caused by coli-infection, yellow dysentery, piglet red dysentery caused by being infected by c-type clostridieum welchii (" in
Doctor trained in Western medicine combination complex treatment piglet bacillary dysentery ", the pig farm Li Wansheng animal doctor, o. 11th in 2011, the 68-69 pages, publication date
On December 31st, 2011).
The piglet refers to pig (" clinical diagnosis and treatment of piglet white scour ", Liu Xingyuan, China poultry from birth to 30kg
Herd animal doctor's digest, the 3rd phase of volume 32 in 2016, page 201, publication date on December 31st, 2016).
Further, in terms of mass parts, pine needle, folium cortex eucommiae, Semen Cuscutae and the fruit of glossy privet proportion relation are as follows: 30-50 parts of pine needle,
20-30 parts of folium cortex eucommiae, 10-20 parts of Semen Cuscutae, 10-20 parts of the fruit of glossy privet.
Further, in terms of mass parts, pine needle, folium cortex eucommiae, Semen Cuscutae and the fruit of glossy privet proportion relation are as follows: 50 parts of pine needle, Du
30 parts of Zhong Ye, 10 parts of Semen Cuscutae, 10 parts of the fruit of glossy privet.
Further, the drying is specially the drying and processing 36h at 40-60 DEG C.
Further, the sieving was specially 80-100 mesh.
Further, the decoction is specially that 6-8 times of steaming of pine needle, folium cortex eucommiae, Semen Cuscutae and glossy privet fruit total quality is added
Distilled water decocts 50-60min.
Further, the microbial bacterial agent is mixed by lactic acid bacteria, saccharomycete and bacillus subtilis.
Further, total bacteria count is 0.5 × 10 in the microbial bacterial agent8-1.0×108cfu/ml。
Further, the anaerobic fermentation is specially the anaerobic fermentation 48-72h at 30-37 DEG C.
Further, the fermented tcm extracting solution, by 30-50 parts of pine needle, 20-30 parts of folium cortex eucommiae, 10-20 parts of Semen Cuscutae,
10-20 parts of the fruit of glossy privet drying and processing 36h at 40-60 DEG C, crushing can pass through 80-100 mesh, addition pine needle, folium cortex eucommiae, Tu
6-8 times of distilled water of silk and glossy privet fruit total quality decocts 50-60min and obtains extracting solution, then through microbial bacterial agent in 30-
Anaerobic fermentation 48-72h at 37 DEG C and obtain, in terms of mass parts, the microbial bacterial agent is by lactic acid bacteria, saccharomycete and withered grass gemma
Bacillus mixes, and total bacteria count is 0.5 × 10 in microbial bacterial agent8-1.0×108cfu/ml。
The beneficial effects of the present invention are:
Fermented tcm extracting solution of the invention is good to the control efficiency of piglet white scour.
Fermented tcm extracting solution of the invention has the function of disease-resistant growth-promoting to growing pigs.
Fermented tcm extracting solution of the invention can be improved feed conversion rate, improve daily gain.
Fermented tcm extracting solution of the invention can be improved pigling immunity.
Fermented tcm extracting solution of the invention is easy to use, can reduce the irritability of livestock and poultry.
Fermented tcm extracting solution of the invention has no toxic side effect, securely and reliably.
Specific embodiment
Illustrated embodiment is to preferably be illustrated to the contents of the present invention, but is not that the contents of the present invention only limit
In illustrated embodiment.So those skilled in the art carry out nonessential change to embodiment according to foregoing invention content
Into and adjustment, still fall within protection scope of the present invention.
Following poplar flower oral liquid is purchased from Chongqing Yong Jian Bioisystech Co., Ltd, and product batch number is；20180801
Immunoglobulin A (IgA) kit is purchased from U.S. TSZ Co., Ltd, product batch number CG2018060192；
Immunoglobulin G (IgG) kit is purchased from U.S. TSZ Co., Ltd, product batch number CG2018060193；
Immunoglobulin M (IgM) kit is purchased from U.S. TSZ Co., Ltd, product batch number CG2018060196.
Embodiment 1
The fermented tcm extracting solution for preventing and treating piglet bacillary dysentery, is by 50 parts of pine needle meal, 30 parts of folium cortex eucommiae, Semen Cuscutae 10
Part, the addition of 10 parts of the fruit of glossy privet (in terms of mass parts) of extracting solution are formed through microbial bacterial agent closed anaerobic fermentation.Specific preparation
Method is as follows:
(1) 50 parts of pine needle meal, 30 parts of folium cortex eucommiae, 10 parts of Semen Cuscutae, 10 parts of the fruit of glossy privet are taken, is respectively placed in baking oven in 60 DEG C
Drying and processing 36h, crushing, which can lead to, to be sieved with 100 mesh sieve, and is then mixed, and is added 600 parts of distilled water (mass parts) and is decocted 60 minutes,
Obtain extracting solution.
(2) lactic acid bacteria, saccharomycete and each 30ml mixing of bacillus subtilis are taken, microbial bacterial agent, and microbial bacteria are obtained
Total bacteria count is 0.6 × 10 in agent8-0.8×108Then microbial bacterial agent is added in extracting solution by cfu/ml, close at 37 DEG C
Close anaerobic fermentation 48 hours.
Wherein, lactic acid bacteria, saccharomycete and bacillus subtilis are respectively adopted corresponding strain and form through conventional method culture.
Determine that total bacteria count reaches 0.6 × 10 in three's mixed bacteria liquid with colony counting method8-0.8×108Until cfu/ml.
Embodiment 2
The fermented tcm extracting solution for preventing and treating piglet bacillary dysentery, is by 40 parts of pine needle meal, 30 parts of folium cortex eucommiae, Semen Cuscutae 15
Part, the addition of 15 parts of the fruit of glossy privet (in terms of mass parts) of extracting solution are formed through microbial bacterial agent closed anaerobic fermentation.Specific preparation
Method is as follows:
(1) 40 parts of pine needle meal, 30 parts of folium cortex eucommiae, 15 parts of Semen Cuscutae, 15 parts of the fruit of glossy privet are taken, is respectively placed in baking oven in 60 DEG C
Drying and processing 36h, it is crushed to lead to and sieves with 100 mesh sieve, then mix, add 800 parts of distilled water (mass parts) and decoct 50 points
Clock obtains extracting solution.
(2) lactic acid bacteria, saccharomycete and each 30ml mixing of bacillus subtilis are taken, microbial bacterial agent, and microbial bacteria are obtained
Total bacteria count is 0.8 × 10 in agent8-1.0×108Then microbial bacterial agent is added in extracting solution by cfu/ml, close at 37 DEG C
Close anaerobic fermentation 72 hours.
Wherein, lactic acid bacteria, saccharomycete and bacillus subtilis are respectively adopted corresponding strain and form through conventional method culture.
Determine that total bacteria count reaches 0.8 × 10 in three's mixed bacteria liquid with colony counting method8-1.0×108Until cfu/ml.
Pharmacodynamic test
Pharmacodynamic test is carried out to fermented tcm extracting solution made from embodiment 1-2, specific as follows:
1. materials and methods
1.1 test drugs: fermented tcm extracting solution made from embodiment 1-2.Control drug: poplar flower oral liquid；
1.2 experimental animals: piglet
1.3 experimental design
Development is neat, growth performance is consistent, weight is 3.6kg or so sodium selenite 180 are chosen, are randomly divided into 6 groups,
Every group 30, respectively experimental group 1-2, control group 1, control group 2,3 and of control group, blank control group；Wherein, experimental group 1-2
Fermented tcm extracting solution 1ml/ head made from embodiment 1-2 is fed respectively, and control group 1 feeds microbial bacterial agent 1ml/ (by cream
Sour bacterium, saccharomycete and bacillus subtilis composition, and total bacteria count is 0.6 × 108Cfu/ml) contain 0.6 × 108The lactic acid of cfu/ml,
Control group 2 feeds poplar flower oral liquid, and control group 3, which is fed, to be accounted for the traditional Chinese medicine powder of feed diet quality 1.0% (traditional Chinese medicine powder is by pine needle meal
40, folium cortex eucommiae 30, Semen Cuscutae 15 and the fruit of glossy privet 15 form, in terms of mass parts).(specific grouping is not administered then for blank control group
1) situation is shown in Table.It successive administration 14 days, is observed continuously again after administration 14 days, the piglet clinical change of timing observation daily；Experimental period
Between swinery be freely eaten, drinking-water, except be administered it is different in addition to other way to manages it is identical.
Off-test counts diarrhea disease incidence, the death rate and the survival rate of every group of piglet, and the results are shown in Table 1.
1 piglet incidence test result of table
| Diarrhea disease incidence/% | The death rate/% | Survival rate/% | |
| Experimental group 1 | 10.00 | 3.33 | 96.67 |
| Experimental group 2 | 10.00 | 6.67 | 93.33 |
| Control group 1 | 13.33 | 6.67 | 93.33 |
| Control group 2 | 26.67 | 13.33 | 86.67 |
| Control group 3 | 26.67 | 10.00 | 90.00 |
| Blank control group | 60.00 | 26.67 | 73.33 |
Remarks: the judgment method of piglet white scour are as follows: piglet arranges yellow loose stools containing ziega or the smelly loose stools of greyish white fishy smell.
As shown in Table 1, the piglet white scour morbidity and mortality of experimental group 1-2 are substantially less than control group 1-3 and blank pair
According to group.Thus it proving, fermented tcm extracting solution of the invention is good to the control efficiency of piglet white scour, meanwhile, have to growing pigs
There is disease-resistant growth-promoting.
Every group randomly selects 10, takes a blood sample on the day before on-test with the 28th day vena cava anterior of test, and recorded,
Test piglet blood sampling is illustrated to measure immune ball immediately according to kit in 2500 turns/min centrifugation 15 minutes after separating serum
Albumin A (IgA), immunoglobulin G (IgG), immunoglobulin M (IgM)；On-test, at the end of weigh feed, piglet respectively
Weight counts piglet daily gain and feed-weight ratio, and the results are shown in Table 2.
2 piglet growth performance of table and immune indexes test result (x ± s, n=10)
As shown in Table 2, compared with blank control group, the piglet feed-weight ratio of experimental group 1-2 is significantly reduced.Thus it demonstrate,proves
Bright, fermented tcm extracting solution of the invention can be improved feed conversion rate, improve daily gain.
As shown in Table 2, the immune state of each group piglet is almost the same before testing, no significant difference.When off-test, by
It has grown up in piglet, immune indexes are varied before relatively testing.Compared with blank control group, experimental group 1-2 when off-test
Immune indexes IgA, IgG, IgM content of piglet is significantly improved.Thus it proves, fermented tcm extracting solution energy of the invention
Enough improve pigling immunity.
Embodiment 3
The fermented tcm extracting solution for preventing and treating piglet bacillary dysentery, is by 45 parts of pine needle meal, 32 parts of folium cortex eucommiae, Semen Cuscutae 12
Part, the addition of 11 parts of the fruit of glossy privet (in terms of mass parts) of extracting solution are formed through microbial bacterial agent closed anaerobic fermentation.Specific preparation
Method is as follows:
(1) 45 parts of pine needle meal, 32 parts of folium cortex eucommiae, 12 parts of Semen Cuscutae, 11 parts of the fruit of glossy privet are taken, is respectively placed in baking oven in 60 DEG C
Drying and processing 36h, it is crushed to lead to and sieves with 100 mesh sieve, then mix, add 800 parts of distilled water (mass parts) and decoct 60 points
Clock obtains extracting solution.
(2) lactic acid bacteria, saccharomycete and each 30ml mixing of bacillus subtilis are taken, microbial bacterial agent, and microbial bacteria are obtained
Total bacteria count is 0.6 × 10 in agent8Then microbial bacterial agent is added in extracting solution by cfu/ml, closing anaerobic is sent out at 37 DEG C
Ferment 72 hours to get the Chinese medicine extract.
Wherein lactic acid bacteria, saccharomycete and bacillus subtilis can be formed using corresponding strain through conventional method culture.
Determine that total bacteria count reaches 0.6 × 10 in three's mixed bacteria liquid with colony counting method8Until cfu/ml.
In addition, it should be understood that although this specification is described in terms of embodiments, but not each embodiment is only wrapped
Containing an independent technical solution, this description of the specification is merely for the sake of clarity, and those skilled in the art should
It considers the specification as a whole, the technical solutions in the various embodiments may also be suitably combined, forms those skilled in the art
The other embodiments being understood that.
Claims (10)
1. the fermented tcm extracting solution for preventing and treating piglet bacillary dysentery, which is characterized in that by pine needle, folium cortex eucommiae, Semen Cuscutae and female
Extracting solution is dried, crushed, being sieved, decocting and to be obtained to loyal son, then obtains through microbial bacterial agent anaerobic fermentation.
2. Chinese medicine extract according to claim 1, which is characterized in that in terms of mass parts, pine needle, folium cortex eucommiae, Semen Cuscutae
With the proportion relation of the fruit of glossy privet are as follows: 30-50 parts of pine needle, 20-30 parts of folium cortex eucommiae, 10-20 parts of Semen Cuscutae, 10-20 parts of the fruit of glossy privet.
3. fermented tcm extracting solution according to claim 2, which is characterized in that in terms of mass parts, pine needle, folium cortex eucommiae, Tu
The proportion relation of silk and the fruit of glossy privet are as follows: 50 parts of pine needle, 30 parts of folium cortex eucommiae, 10 parts of Semen Cuscutae, 10 parts of the fruit of glossy privet.
4. fermented tcm extracting solution according to claim 1,2 or 3, which is characterized in that the drying is specially in 40-60
Drying and processing 36h at DEG C.
5. fermented tcm extracting solution according to claim 1,2,3 or 4, which is characterized in that the sieving was specially 80-
100 meshes.
6. according to claim 1, fermented tcm extracting solution described in 2,3,4 or 5, which is characterized in that the decoction is specially to add
Enter 6-8 times of distilled water of pine needle, folium cortex eucommiae, Semen Cuscutae and glossy privet fruit total quality, decocts 50-60min.
7. according to claim 1, fermented tcm extracting solution described in 2,3,4,5 or 6, which is characterized in that the microbial bacterial agent
It is mixed by lactic acid bacteria, saccharomycete and bacillus subtilis.
8. fermented tcm extracting solution according to claim 1,2,3,4,5,6 or 7, which is characterized in that the microbial bacteria
Total bacteria count is 0.5 × 10 in agent8-1.0×108cfu/ml。
9. according to claim 1, fermented tcm extracting solution described in 2,3,4,5,6,7 or 8, which is characterized in that the anaerobism hair
Ferment is specially the anaerobic fermentation 48-72h at 30-37 DEG C.
10. according to claim 1, fermented tcm extracting solution described in 2,3,4,5,6,7,8 or 9, which is characterized in that by pine needle
30-50 parts, 20-30 parts of folium cortex eucommiae, 10-20 parts of Semen Cuscutae, 10-20 parts of the fruit of glossy privet drying and processing 36h at 40-60 DEG C, crushing,
Cross 80-100 mesh, be added pine needle, folium cortex eucommiae, Semen Cuscutae and glossy privet fruit total quality 6-8 times of distilled water, decoction 50-60min
Extracting solution, then through microbial bacterial agent at 30-37 DEG C anaerobic fermentation 48-72h and obtain, in terms of mass parts, micro- life
Object microbial inoculum is mixed by lactic acid bacteria, saccharomycete and bacillus subtilis, and total bacteria count is 0.5 × 10 in microbial bacterial agent8-
1.0×108cfu/ml。
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| CN110786420A (en) * | 2019-12-11 | 2020-02-14 | 重庆市畜牧科学院 | Feed additive for piglets |
| CN113475633A (en) * | 2021-06-07 | 2021-10-08 | 吉林省农业科学院 | Fermented Chinese herbal medicine, method, feed and application applied to production of weaned piglets |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110786420A (en) * | 2019-12-11 | 2020-02-14 | 重庆市畜牧科学院 | Feed additive for piglets |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110786420A (en) * | 2019-12-11 | 2020-02-14 | 重庆市畜牧科学院 | Feed additive for piglets |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110786420A (en) * | 2019-12-11 | 2020-02-14 | 重庆市畜牧科学院 | Feed additive for piglets |
| CN113475633A (en) * | 2021-06-07 | 2021-10-08 | 吉林省农业科学院 | Fermented Chinese herbal medicine, method, feed and application applied to production of weaned piglets |
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