CN109699681B - Bacillus pumilus ZR3-3 for preventing and treating bacterial diseases and application thereof - Google Patents
Bacillus pumilus ZR3-3 for preventing and treating bacterial diseases and application thereof Download PDFInfo
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Abstract
The invention relates to a biocontrol microbial inoculum, wherein the effective active ingredient of the biocontrol microbial inoculum consists of Bacillus pumilus (Bacillus pumilus) ZR 3-3. The method is characterized in that: the biocontrol microbial inoculum is an aqueous agent and comprises the following components in percentage by weight: bacillus pumilus ZR 3-3: 89.5 to 99.8 percent; sodium dodecyl sulfate: 0.1-10%; potassium sorbate: 0.1 to 0.5 percent. When the water aqua is applied, the water aqua is irrigated to roots to prevent and control soft rot of Chinese cabbages and bacterial wilt of hot peppers, and is uniformly sprayed on rice leaf surfaces to prevent and control bacterial leaf blight of rice and bacterial leaf streak of rice. The advantages are that: the purpose of preventing and treating the plant bacterial diseases by using the bio-control fungicide is achieved by introducing the bacillus pumilus ZR3-3 which is safe to human and livestock and has better prevention and treatment effects on various plant bacterial diseases; the dosage of chemical agents is reduced; meanwhile, the ecological environment is improved, and the residual quantity of chemical agents in agricultural products is reduced.
Description
Technical Field
The invention relates to a biocontrol microbial inoculum and application thereof, in particular to a bacillus pumilus ZR3-3 and application thereof in preventing and treating plant bacterial diseases.
Background
The bacterial diseases are diseases caused by pathogenic bacteria infection, such as soft rot, bacterial wilt, bacterial leaf blight and the like. Bacteria that attack plants are bacilli, most of which have 1 to several flagella, and can invade through natural orifices (stomata, skin pores, water pores, etc.) and wounds, and spread by running water, rain water, insects, etc., and easily cause diseases in the case of passing winter in disease residues, seeds, and soil under high temperature and high humidity conditions. The symptoms of the bacterial diseases are manifested by wilting, rotting, perforation and the like, bacterial mucus overflows from the disease parts when wet weather occurs in the later period of disease, and the disease has obvious foul smell and is the characteristic of the bacterial diseases. At present, the production of preventing and treating plant bacterial diseases mainly depends on chemical pesticides such as thiacumidine, copper preparations and the like, so that not only is the ecological environment seriously polluted, but also the residues of toxic chemical substances in agricultural products are directly increased, and the serious harm is brought to the human health. In order to achieve the purposes of effectively controlling plant diseases and reducing the use amount of chemical pesticides, methods for controlling plant diseases by using biological control technologies are receiving more and more attention from governments and people. Biological control technology mainly utilizes biological diversity, and can continuously control plant diseases by releasing or introducing beneficial microorganisms in the nature. The research of using the biocontrol microbial inoculum to prevent and treat plant bacterial diseases is carried out at home and abroad since 1950, and the biocontrol microbial inoculum mainly comprises: bacillus (Bacillus), pseudomonas (pseudomonas), and the like.
A novel biocontrol strain ZR3-3 is obtained by separating and purifying soil samples collected by agricultural academy of sciences of Jiangsu province from Tibet grassland. The results of the previous studies show that: the biocontrol strain ZR3-3 has good control effect on various bacterial diseases (soft rot, bacterial wilt, bacterial leaf blight, bacterial streak, black rot and the like) and fungal diseases (such as anthracnose, banded sclerotial blight, blight and the like) in production. The strain ZR3-3 is identified as Bacillus pumilus (Bacillus pumilus) by the detection and identification of the institute of microbiology of Chinese academy of sciences and the comprehensive analysis of experimental data such as cell morphology, physiological and biochemical characteristics, 16S rRNA gene sequence and the like. Bacillus pumilus ZR3-3 has been registered in China general microbiological culture Collection center at 11.15.2018 with the address of No. 3 Siro-1 of Kyowa, Chaoyang, Beijing, and the collection number of the microorganism research institute of Chinese academy of sciences is CGMCC No. 16740.
Bacillus pumilus has been reported to primarily control fungal diseases including verticillium wilt of cotton (Liu et al, 2018, proceedings of Henan university), Phytophthora sojae (Cao Shu et al, 2015, Ph D.J. university of Anhui), tobacco blackleg (Wang Jing et al, 2010, Chinese tobacco science), Alternaria alternata and powdery mildew (Wang Jing et al, 2015 D.AN. ecology), neck blast of rice (Zhu Mei et al, 2007, Anhui agricultural science), rice sheath blight (Jasdep ChathPadaria et al, 2016, Journal of Basic Microbiology; Amin Rostami et al, 2017, Pesticide Biotechnology and Physiology), sclerotinia sclerotiorum (Manoj Kaushal et al, 2017, root knot nematode, Sha et al, Johnik Biotechnology and Physiog et al, Johnia japonica, Ha et al, Johniki.p. Biotechnology and Phymatology, Ph., 2017, Huang. Biotechnology and Phymato, Ha., Yongg et al, Johnik, Johnia, Ha., Yongg et al, Johnik et al, Johnik et al, Biotechnology, phytoparasitica), Penicillium (Haijiao Yan et al, 2018, world journal of Microbiology and Biotechnology), and the like. The main control objects of the existing Bacillus pumilus are fungal diseases including rice blast, wheat heterodera avenae wollen, apple alternaria leaf spot, strawberry anthracnose, wheat sharp eyespot, fruit tree rot and the like (patent application No.: CN201711171896. X; CN 201310046055.1; CN 201510502270.7; CN 201510312295.0; CN 201510219845.4; CN 201410339603.4). In addition, some compost or microorganism straw decomposing agents contain bacillus pumilus (patent application number: 201310560992.9) which can decompose straws and simultaneously have biological control function, and indoor test results show that the growth of rhizoctonia solani and rice bacterial blight can be inhibited.
At present, 2 reports of using Bacillus pumilus to prevent and treat tomato bacterial wilt at home and abroad (1, Lianli et al, 2009, university of agriculture and forestry, Fujian, 2, Min Shen et al, 2017, Acta Agriculture Scandinavica, Section B-Soil and Plant Science) have the action principle of mainly inducing Plant resistance and weakening germ toxicity. The mechanism of action and the target pathogenic bacteria of the Bacillus pumilus ZR3-3 obtained by the applicant are completely different from those of the Bacillus pumilus ZR3-3, which are specifically as follows: 1. the bacillus pumilus ZR3-3 living bacteria can directly inhibit the growth of bacterial diseases indoors and outdoors, and is different from the reported action mechanisms of induced resistance, weakened germ toxicity and the like; 2. as is well known, Bacillus pumilus is a biocontrol bacterium with wider production and application. It is to be emphasized that: within this large family of B.pumilus, the targets of action of the different strains are not completely identical. One strain of bacillus pumilus has an inhibitory effect on pathogenic bacteria, and cannot represent that all the bacillus pumilus have an inhibitory effect on pathogenic bacteria. The Bacillus pumilus ZR3-3 separated by the applicant has control effects on tomato bacterial wilt and pepper bacterial wilt, and other Bacillus pumilus do not necessarily have control effects on pepper bacterial wilt. Although the pepper bacterial wilt and the tomato bacterial wilt are both of the genus Laurel, infecting different hosts can generate different specialities such as specialities of speciality, pathogenicity and the like. In other words, it has been reported in the literature that Bacillus pumilus for controlling tomato bacterial wilt cannot necessarily control pepper bacterial wilt.
The Bacillus pumilus ZR3-3(Bacillus pumilus) is a new strain obtained by sampling and separating by the applicant, can effectively prevent and control bacterial diseases such as Chinese cabbage soft rot, pepper bacterial wilt, rice bacterial leaf blight, rice bacterial streak and the like, has no relevant report at home and abroad, and has creativity and novelty.
Disclosure of Invention
The invention aims to provide a biocontrol strain, namely Bacillus pumilus ZR3-3 with better application prospect, which has been registered in the China general microbiological culture Collection center at 11 and 15 days in 2018, wherein the address is No. 3 of the Beijing university Hokko No.1 of sunward area, and the preservation number is CGMCC No.16740 at the institute of microbiology of the China academy of sciences.
The invention provides a biocontrol microbial inoculum, wherein the effective active ingredient of the biocontrol microbial inoculum consists of bacillus pumilus ZR 3-3.
The biocontrol microbial inoculum comprises three antibacterial related substances, namely protease, siderophin and chitinase, which are secreted by bacillus pumilus ZR 3-3.
The biocontrol microbial inoculum is an aqueous agent.
The water aqua of the invention is characterized in that: the components and the weight percentage content are as follows: bacillus pumilus ZR 3-3: 89.5 to 99.8 percent; sodium dodecyl sulfate: 0.1-10%; potassium sorbate: 0.1 to 0.5 percent; the pH value is 6.5-7.0.
The aqueous agent disclosed by the invention is used for preventing and treating soft rot of Chinese cabbage and pepper bacterial wilt by root irrigation, and is used for preventing and treating bacterial leaf blight of rice and bacterial leaf streak of rice by uniformly spraying on rice leaf surfaces.
The invention has the advantages that: a biocontrol strain-bacillus pumilus ZR3-3 which is safe to human and livestock and has better application prospect is developed, and biocontrol resources are enriched; the biocontrol microbial inoculum can be used for preventing and treating various plant bacterial diseases, reducing the using amount of chemical agents in production, improving the ecological environment and reducing the residual amount of the chemical agents in agricultural products.
Drawings
The invention is further illustrated with reference to the following figures and examples.
FIG. 1 shows the inhibitory effect of Bacillus pumilus ZR3-3 on pathogenic bacteria
FIG. 2 shows the protease produced by Bacillus pumilus ZR 3-3.
FIG. 3 shows the production of siderophore by Bacillus pumilus ZR 3-3.
FIG. 4 shows the chitinase production by Bacillus pumilus ZR 3-3.
In the figure, A: bacillus pumilus ZR 3-3; b: chinese cabbage soft rot; c: bacterial wilt of capsicum; d: rice bacterial blight; e: bacterial leaf streak disease of rice.
The specific implementation mode is as follows:
examples 1,
Inhibition of pathogenic bacteria by Bacillus pumilus ZR3-3
A. Pathogenic bacteria to be tested
The tested bacteria comprise cabbage soft rot, pepper ralstonia solanacearum, rice leaf blight and rice bacterial streak germ, which are provided by the plant protection research institute of agricultural academy of sciences of Jiangsu province. Culturing with LB culture solution (peptone 10.0g, yeast powder 5.0g, NaCl 10g, distilled water 1000mL) at 28 deg.C under 150rpm shaking for 2d, diluting with sterile water to 10%8cfu/mL for use.
B. The test method comprises the following steps:
activating Bacillus pumilus ZR3-3 screened by agricultural academy of sciences of Jiangsu province on LB culture medium, transferring into 50mL LB culture solution, culturing at 28 deg.C under shaking at 150rpm for 2d, diluting with sterile water to 10%8cfu/mL for use.
Sucking 5 mu L of bacillus pumilus ZR3-3 bacterial liquid, dotting the liquid at the center of an LB flat plate, culturing at 28 ℃ for 48h, spraying pathogenic bacteria diluent, culturing at 28 ℃ overnight, and investigating the diameter of a bacteriostatic circle. Each treatment was repeated 3 times and averaged. And setting clear water as a reference.
C. And (3) test results:
the test results are shown in FIG. 1. The bacillus pumilus ZR3-3 bacterial liquid has certain inhibition effect on 4 kinds of bacteria to be tested, and the diameter of an inhibition zone for cabbage soft rot germs is 70.2 mm; the diameter of a bacteriostasis zone for the pseudomonas solanacearum is 31.7 mm; the diameter of the inhibition zone for rice bacterial blight is 27.5 mm; the diameter of the inhibition zone for rice bacterial streak germ is 66.2 mm.
As shown in fig. 1.
Example 2
Production of antibacterial related substance from metabolic secretion of Bacillus pumilus ZR3-3
Test materials: protease detection medium: 100g of skimmed milk powder and 20g of agar, and the volume is up to 1000 mL. And (3) detecting a siderophore culture medium: CAS 60.5mg, 10mL ferric iron solution (1 mmol.L)-1FeCl3·6H2O), HDTMA 72.9mg, agar 20g, constant volume to 1000mL, pH 7.0. Chitinase assay medium: chitin 15g, MgSO4·7H2O 0.5g，FeS04·7H2O0.01g，K2HP040.7g，KH2P040.3g of agar and 20g of agar, and the volume is up to 1000mL, and the pH value is 7.0-7.2. Cellulase detection culture medium: 10g of peptone, 10g of yeast powder, 10g of sodium carboxymethylcellulose, 5g of NaCl and KH2PO41g of agar and 20g of agar, and the volume is up to 1000mL, and the pH value is 7.0.
The Bacillus pumilus ZR3-3 strain was prepared as in example 1.5 mu L of Bacillus pumilus ZR3-3 bacterial liquid is inoculated to the center of each detection medium plate, and each treatment is repeated for 3 times. Culturing at 28 deg.C for 3-7 days, and observing.
And (3) protease detection: the presence or absence of the transparent ring was observed. If a transparent ring is generated, the antagonistic bacteria can secrete the protease.
Detecting siderophore: after culturing at 28 ℃ for 7-10 days, observing whether yellow halo is generated. If a yellow halo is produced, the antagonistic bacteria can secrete the siderophore.
Chitinase detection: the presence or absence of the transparent ring was observed. If a transparent ring is generated, the antagonistic bacteria can secrete chitinase and degrade chitin.
And (3) cellulase detection: the colony was observed for the presence of a transparent ring around it. If a transparent circle is generated, the antagonistic bacteria can secrete the cellulase.
The test result shows that: bacillus pumilus ZR3-3Secreting cellulase; the protein in the detection plate can be used for forming a transparent ring around the colony by secreting protease (figure 2); can secrete siderophore and detect Fe in the plate3+Binding, forming an orange halo around the colonies (fig. 3); chitinase was secreted and a clear ring was formed around the colony using chitin in the assay plate (FIG. 4).
As shown in fig. 2, 3 and 4.
Example 3
Preparation of Bacillus pumilus ZR3-3 aqueous solution
A. Preparing and activating strains.
LB medium (same as example 1) was sterilized by adding agar and slant-slant culture. Inoculating Bacillus pumilus ZR3-3, and culturing at 28 deg.C for 2 days.
B. And (5) liquid fermentation.
Primary fermentation: transferring the cultured test tube slant with an inoculating loop to 300mL LB culture solution. Shaking and culturing at 28 deg.C and 150rpm for 2 d.
Secondary fermentation: the formula of the culture medium is as follows: 1% of soybean cake powder, 0.5% of soybean meal, 0.15% of yeast extract, 1% of corn flour, 0.5% of corn steep liquor, 0.5% of cane sugar, 0.05% of NaCl, CaCO30.18 percent. pH 7.0 before sterilization. 1 ton of material was charged into a 2 ton fermentor. Sterilizing at 121 deg.C for 30 min. And (3) cooling to about 30 ℃, and inoculating 300mL of the primary fermentation strain liquid. The liquid fermentation temperature is 32 ℃, and the stirring speed is 300 rpm. The ventilation rate is 1: 1.5. The fermentation period was 18 hours.
And (3) three-stage fermentation: the culture medium formula is the same as the secondary fermentation. 10 tons of feed are fed into a 15 ton fermenter. Sterilizing at 121 deg.C for 30 min. And (3) inoculating 1 ton of the secondary fermentation strain liquid when cooling to about 30 ℃. The liquid fermentation temperature is 32 ℃, and the stirring speed is 300 rpm. The ventilation rate is 1: 1.5. The fermentation period is about 24 hours, and the mixture is put into a tank.
C. And (4) preparing an aqueous agent.
Putting the fermentation liquor into a storage tank, adding 0.1-10% of sodium dodecyl sulfate and 0.1-0.5% of potassium sorbate, and uniformly stirring. The pH value of the fermentation liquor is 6.5-7.0, and the number of viable bacteria is 109cfu/mL. Filling into a water aqua of Bacillus pumilus ZR 3-3.
Example 4
Test for preventing and treating Chinese cabbage soft rot by bacillus pumilus ZR3-3 aqueous solution
Test site: carried out in Jiangsu province, Ganxingu region of Lianyung City, Lianhong province.
The test varieties are as follows: xia Feng, also called Fu Bao, is provided by vegetable research institute of agricultural academy of sciences in Jiangsu province.
Test and control agents: aqueous Bacillus pumilus ZR3-3 was provided in example 3. The control agent is 2% kasugamycin wettable powder, purchased from Chunlei biological pharmaceutical industry Co., Ltd, Yanbian, Jilin province, and used according to the recommended dosage in the field.
The test method comprises the following steps: irrigating roots of the Chinese cabbage for 10 days, 17 days and 25 days respectively for 1 time, and irrigating 250ml of the pesticide liquid for each plant. The experiment was set to 5 treatments: : 100 times of diluent of bacillus pumilus ZR3-3 aqueous solution, 200 times of diluent of ZR3-3 aqueous solution, 750 times of diluent of 2% kasugamycin wettable powder, 1500 times of diluent of 2% kasugamycin wettable powder and clear water for comparison. 4 times of repetition, 20 cells in total, and the area of each cell is 20m2And are randomly arranged. Randomly sampling in each cell at 35d and 55d after the administration, investigating 50 Chinese cabbages, investigating the disease incidence, and calculating the disease incidence index and the prevention effect. Disease grading criteria are as follows:
level 0: no symptoms;
level 1: the leaves begin to appear water stain-like brown scabs;
and 3, level: the base of the leaf at the bottom 1-2 has obvious scab;
and 5, stage: the outer leaves are 1/3-1/2 rotten;
and 7, stage: the outer leaves are all rotten;
and 9, stage: the whole plant is rotten.
Disease index ═ number of onset of disease at each stage × representative value at each stage)/(total number of investigated plants × highest representative value) ] × 100
Control effect (%) < control disease index > < 100%
The test results are shown in Table 2. After the medicine is taken for 35 days, the control effect of the bacillus pumilus ZR3-3 aqueous solution 100 times of the diluent is the highest and is 83.10 percent, the control effect of the control medicament 2% kasugamycin wettable powder 750 times of the diluent is the second and is 75.35 percent, and the two are obviously different. The control effect of the 200-time diluent of the bacillus pumilus ZR3-3 aqueous solution is equivalent to that of the 1500-time diluent of 2% kasugamycin wettable powder. 55 days after the drug is applied, the disease index of the clear water contrast is obviously increased, the control effect of the bacillus pumilus ZR3-3 aqueous solution 100 times of the diluent is 81.48 percent, the control effect of the 2 percent kasugamycin wettable powder 750 times of the diluent is 75.21 percent, and the two are still obviously different. The results show that: the bacillus pumilus ZR3-3 has obvious effect of preventing and treating Chinese cabbage soft rot.
TABLE 2 field control effect of Bacillus pumilus ZR3-3 aqua on cabbage soft rot
Note: different lower case letters in the same column indicate significant differences.
Example 5
Bacillus pumilus ZR3-3 aqueous solution field plot test for preventing and treating tomato and pepper bacterial wilt
Test site: facility greenhouse of agricultural and sideline product distribution limited company in Suzhou of Jiangsu province.
The test varieties are as follows: xia fen No. 3, Su jiao No. 17, provided by vegetable research institute of agricultural academy of sciences of Jiangsu province.
Test agents: the bacillus pumilus ZR3-3 aqueous solution is provided in example 3, and the control agent is 3% Zhongshengmycin wettable powder purchased from Shenzhen Nuopuxin agrichemical Co., Ltd and used according to the recommended dosage in the field.
The test method comprises the following steps: selecting 2 greenhouse greenhouses with the tomato bacterial wilt and pepper bacterial wilt which are relatively serious in natural morbidity as test greenhouses. The test seedlings are subjected to two stages of channel plug seedling and field planting according to conventional operation. When the hole tray is used for sowing, diluting the bacillus pumilus ZR3-3 aqueous solution according to the proportion of 1: 50, and irrigating 50ml of the solution in each hole; during planting and transplanting, pouring 200 ml of water aqua of Bacillus pumilus ZR3-3 into roots according to the ratio of 1: 100; and irrigating roots of the bacillus pumilus ZR3-3 aqueous solution according to the proportion of 1: 100 every 15 days after planting, wherein 500 ml of roots are irrigated to each seedling. Test agents: bacillus pumilus ZR3-3 water agent, 3% zhongshengmycin wettable powder and clear water as reference. Each treatment cell is about 500 seedlings, the treatment cell is repeated for 2 times, and the treatment cells are randomly distributed. And (5) investigating the disease condition when the tomatoes and the peppers are attacked in the control plot, and calculating the control effect.
Incidence rate ═ number of diseased strains in survey cell/number of total strains investigated × 100
Control efficacy (incidence of treatment cells-incidence of control cells)/incidence of control cells
The test results are shown in Table 3. The disease incidence of the tomato bacterial wilt in a control disease field is 18.46 percent, the field control effect of the bacillus pumilus ZR3-3 aqueous solution on the tomato bacterial wilt is 72.70 percent, the control effect of the 3 percent zhongshengmycin wettable powder is 59.26 percent, and the two are obviously different. The incidence rate of the pepper bacterial wilt in a control disease field is 53.27 percent, the field control effect of the bacillus pumilus ZR3-3 aqueous solution on the pepper bacterial wilt is 71.75 percent, the control effect of the 3 percent zhongshengmycin wettable powder is 46.05 percent, and the two are obviously different. The test result shows that: the bacillus pumilus ZR3-3 aqua can effectively prevent and treat tomato bacterial wilt and pepper bacterial wilt.
TABLE 3 field control effect of Bacillus pumilus ZR3-3 aqua on tomato and pepper bacterial wilt
Note: different lower case letters in the same column indicate significant differences.
Example 6
Bacillus pumilus ZR3-3 aqueous solution for preventing and treating bacterial streak and bacterial blight of rice in field
Test site: test field of agricultural science institute of Jiangsu province.
Test agents: the bacillus pumilus ZR3-3 aqueous solution is provided in example 3, and the control agent is 20% thifluzazole wettable powder, available from qiangjiang longwan chemical industry ltd), used according to the instructions.
Spraying the pesticide on the leaves in the early stage of bacterial leaf blight and bacterial streak disease (sporadic disease in the field), and performing secondary control at the interval of about 10 days. The investigation method comprises the following steps: at the initial stage of disease onset, 4 points were randomly selected per cell, 5 points per point, and 3 leaves at the uppermost part of each tillering were investigated. The disease index was investigated again after the initial administration of 21 days.
Grading standard: grade 0, no scab; grade 1, the scab occupies less than 5 percent of the leaf area; grade 3, the lesion area accounts for 6 to 15 percent of the leaf area; grade 5, the lesion area accounts for 16 to 30 percent of the leaf area; 7, the lesion area accounts for 31 to 50 percent of the leaf area; grade 9, the lesion area accounts for more than 50% of the leaf area.
The disease index ═ Σ (number of onset at each stage × representative value at each stage)/(total number of examined leaves × highest representative value) × 100;
the preventing and treating effect is [1- (after-drug disease index in the treatment area x before-drug disease index in the control area)/(before-drug disease index in the treatment area x after-drug disease index in the control area) ] × 100%.
The test results are shown in Table 4. The control agents respectively have the control effects on the bacterial leaf streak and the bacterial blight of rice of 48.18 percent and 65.39 percent, the Bacillus pumilus ZR3-3 aqueous solution accounts for 33.3-50ml of the dosage per mu, the control effects on the bacterial leaf streak and the bacterial blight of rice of 50.24-54.24 percent and 60.25-65.39 percent, and the control effects on the bacterial leaf streak and the bacterial blight of rice are equivalent to the control agents; if the dosage of each mu is 100ml, the control effect on the bacterial leaf streak and bacterial leaf blight of rice is 61.99 percent and 73.08 percent respectively; the control effect is obvious and is obviously different from the control medicament.
TABLE 4 field control effect of Bacillus pumilus ZR3-3 aqua on bacterial streak and bacterial blight of rice
Claims (3)
1. A biological control agent is characterized in that: the effective active ingredients in the biological control agent consist of Bacillus pumilus (Bacillus pumilus) ZR3-3 CGMCC No. 16740; metabolites secreted by the bacillus pumilus ZR3-3 comprise three antibacterial related substances of protease, siderophin and chitinase; the biocontrol microbial inoculum is an aqueous agent.
2. The biocontrol microbial inoculum of claim 1 wherein: the components and the weight percentage content are as follows: bacillus pumilus: 89.5 to 99.8 percent; sodium dodecyl sulfate: 0.1-10%; potassium sorbate: 0.1 to 0.5 percent.
3. The use of the biocontrol microbial inoculum according to claim 1, characterized in that: irrigating roots to prevent and control soft rot of Chinese cabbage and bacterial wilt of hot pepper, and spraying on leaves of rice to prevent and control bacterial leaf blight of rice and bacterial leaf streak of rice.
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| CN104982459A (en) * | 2015-06-09 | 2015-10-21 | 苏州市农业科学院 | Bacillus pumilus N103-1 and application thereof |
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