CN108739380A - 一种白及组培苗一次性成苗的方法 - Google Patents
一种白及组培苗一次性成苗的方法 Download PDFInfo
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- CN108739380A CN108739380A CN201810502968.2A CN201810502968A CN108739380A CN 108739380 A CN108739380 A CN 108739380A CN 201810502968 A CN201810502968 A CN 201810502968A CN 108739380 A CN108739380 A CN 108739380A
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- Prior art keywords
- seedling
- culture
- bottle
- bletilla
- tissue
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Links
- 241001313855 Bletilla Species 0.000 title claims abstract description 41
- 238000000034 method Methods 0.000 title claims abstract description 21
- 230000015572 biosynthetic process Effects 0.000 title claims abstract description 18
- 241001597008 Nomeidae Species 0.000 claims abstract description 17
- 230000035755 proliferation Effects 0.000 claims abstract description 16
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 14
- 230000001939 inductive effect Effects 0.000 claims abstract description 14
- 239000005648 plant growth regulator Substances 0.000 claims description 25
- 235000015097 nutrients Nutrition 0.000 claims description 21
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical group O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 18
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 13
- 229910001868 water Inorganic materials 0.000 claims description 13
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 claims description 12
- FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Chemical compound [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 claims description 12
- 238000012258 culturing Methods 0.000 claims description 11
- 239000002775 capsule Substances 0.000 claims description 10
- GPXLRLUVLMHHIK-UHFFFAOYSA-N forchlorfenuron Chemical compound C1=NC(Cl)=CC(NC(=O)NC=2C=CC=CC=2)=C1 GPXLRLUVLMHHIK-UHFFFAOYSA-N 0.000 claims description 9
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims description 6
- ZGTMUACCHSMWAC-UHFFFAOYSA-L EDTA disodium salt (anhydrous) Chemical compound [Na+].[Na+].OC(=O)CN(CC([O-])=O)CCN(CC(O)=O)CC([O-])=O ZGTMUACCHSMWAC-UHFFFAOYSA-L 0.000 claims description 6
- 239000004471 Glycine Substances 0.000 claims description 6
- SQUHHTBVTRBESD-UHFFFAOYSA-N Hexa-Ac-myo-Inositol Natural products CC(=O)OC1C(OC(C)=O)C(OC(C)=O)C(OC(C)=O)C(OC(C)=O)C1OC(C)=O SQUHHTBVTRBESD-UHFFFAOYSA-N 0.000 claims description 6
- 239000007836 KH2PO4 Substances 0.000 claims description 6
- 229910004619 Na2MoO4 Inorganic materials 0.000 claims description 6
- PVNIIMVLHYAWGP-UHFFFAOYSA-N Niacin Chemical compound OC(=O)C1=CC=CN=C1 PVNIIMVLHYAWGP-UHFFFAOYSA-N 0.000 claims description 6
- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 claims description 6
- 239000001110 calcium chloride Substances 0.000 claims description 6
- 229910001628 calcium chloride Inorganic materials 0.000 claims description 6
- JZCCFEFSEZPSOG-UHFFFAOYSA-L copper(II) sulfate pentahydrate Chemical compound O.O.O.O.O.[Cu+2].[O-]S([O-])(=O)=O JZCCFEFSEZPSOG-UHFFFAOYSA-L 0.000 claims description 6
- XLYOFNOQVPJJNP-ZSJDYOACSA-N heavy water Substances [2H]O[2H] XLYOFNOQVPJJNP-ZSJDYOACSA-N 0.000 claims description 6
- CDAISMWEOUEBRE-GPIVLXJGSA-N inositol Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@H](O)[C@@H]1O CDAISMWEOUEBRE-GPIVLXJGSA-N 0.000 claims description 6
- 229960000367 inositol Drugs 0.000 claims description 6
- SURQXAFEQWPFPV-UHFFFAOYSA-L iron(2+) sulfate heptahydrate Chemical compound O.O.O.O.O.O.O.[Fe+2].[O-]S([O-])(=O)=O SURQXAFEQWPFPV-UHFFFAOYSA-L 0.000 claims description 6
- WRUGWIBCXHJTDG-UHFFFAOYSA-L magnesium sulfate heptahydrate Chemical compound O.O.O.O.O.O.O.[Mg+2].[O-]S([O-])(=O)=O WRUGWIBCXHJTDG-UHFFFAOYSA-L 0.000 claims description 6
- CDUFCUKTJFSWPL-UHFFFAOYSA-L manganese(II) sulfate tetrahydrate Chemical compound O.O.O.O.[Mn+2].[O-]S([O-])(=O)=O CDUFCUKTJFSWPL-UHFFFAOYSA-L 0.000 claims description 6
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 6
- 235000001968 nicotinic acid Nutrition 0.000 claims description 6
- 229960003512 nicotinic acid Drugs 0.000 claims description 6
- 239000011664 nicotinic acid Substances 0.000 claims description 6
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 claims description 6
- CDAISMWEOUEBRE-UHFFFAOYSA-N scyllo-inosotol Natural products OC1C(O)C(O)C(O)C(O)C1O CDAISMWEOUEBRE-UHFFFAOYSA-N 0.000 claims description 6
- 239000011684 sodium molybdate Substances 0.000 claims description 6
- TVXXNOYZHKPKGW-UHFFFAOYSA-N sodium molybdate (anhydrous) Chemical compound [Na+].[Na+].[O-][Mo]([O-])(=O)=O TVXXNOYZHKPKGW-UHFFFAOYSA-N 0.000 claims description 6
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 claims description 6
- 229910000368 zinc sulfate Inorganic materials 0.000 claims description 6
- 239000011686 zinc sulphate Substances 0.000 claims description 6
- 229960002523 mercuric chloride Drugs 0.000 claims description 5
- LWJROJCJINYWOX-UHFFFAOYSA-L mercury dichloride Chemical compound Cl[Hg]Cl LWJROJCJINYWOX-UHFFFAOYSA-L 0.000 claims description 5
- 239000008223 sterile water Substances 0.000 claims description 5
- 230000001954 sterilising effect Effects 0.000 claims description 5
- 238000004659 sterilization and disinfection Methods 0.000 claims description 5
- 230000008093 supporting effect Effects 0.000 claims description 5
- 239000002352 surface water Substances 0.000 claims description 5
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- 238000005286 illumination Methods 0.000 claims description 4
- LFDGRWDETVOGDT-UHFFFAOYSA-N 1h-pyrrole;hydrochloride Chemical compound Cl.C=1C=CNC=1 LFDGRWDETVOGDT-UHFFFAOYSA-N 0.000 claims description 3
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 3
- -1 hydrochloric acid sulphur Amine Chemical class 0.000 claims description 3
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- 239000011747 thiamine hydrochloride Substances 0.000 claims description 3
- DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 claims description 3
- 238000007670 refining Methods 0.000 claims description 2
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- JTEDVYBZBROSJT-UHFFFAOYSA-N indole-3-butyric acid Chemical compound C1=CC=C2C(CCCC(=O)O)=CNC2=C1 JTEDVYBZBROSJT-UHFFFAOYSA-N 0.000 description 7
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- 235000012871 Arctostaphylos uva ursi Nutrition 0.000 description 1
- 241001313857 Bletilla striata Species 0.000 description 1
- 244000025254 Cannabis sativa Species 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 239000005979 Forchlorfenuron Substances 0.000 description 1
- 208000034507 Haematemesis Diseases 0.000 description 1
- 208000000616 Hemoptysis Diseases 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
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- 230000007774 longterm Effects 0.000 description 1
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- 239000000463 material Substances 0.000 description 1
- YGGXZTQSGNFKPJ-UHFFFAOYSA-N methyl 2-naphthalen-1-ylacetate Chemical compound C1=CC=C2C(CC(=O)OC)=CC=CC2=C1 YGGXZTQSGNFKPJ-UHFFFAOYSA-N 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
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- 238000004161 plant tissue culture Methods 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
Description
Claims (4)
Priority Applications (1)
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CN201810502968.2A CN108739380B (zh) | 2018-05-23 | 2018-05-23 | 一种白及组培苗一次性成苗的方法 |
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CN201810502968.2A CN108739380B (zh) | 2018-05-23 | 2018-05-23 | 一种白及组培苗一次性成苗的方法 |
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CN108739380A true CN108739380A (zh) | 2018-11-06 |
CN108739380B CN108739380B (zh) | 2020-09-15 |
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CN201810502968.2A Active CN108739380B (zh) | 2018-05-23 | 2018-05-23 | 一种白及组培苗一次性成苗的方法 |
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Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109964797A (zh) * | 2019-03-22 | 2019-07-05 | 浙江大学 | 促进白及假鳞茎增殖/膨大的方法 |
CN112154919A (zh) * | 2020-11-09 | 2021-01-01 | 广西壮族自治区农业科学院 | 一种诱导七叶一枝花愈伤组织直接成苗的培养基及方法 |
CN115644058A (zh) * | 2022-09-23 | 2023-01-31 | 广西壮族自治区农业科学院 | 一种促进兰科植物种子快速成苗的方法 |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH05148115A (ja) * | 1991-07-08 | 1993-06-15 | Sanko Kk | ランの簡便な播種・発芽方法及び移植方法 |
CN103299903A (zh) * | 2013-05-23 | 2013-09-18 | 广西壮族自治区中国科学院广西植物研究所 | 一种以假鳞茎诱导假鳞茎的白芨高效繁殖方法 |
KR20150103879A (ko) * | 2014-03-04 | 2015-09-14 | 고려대학교 산학협력단 | 난 유묘의 생산방법 |
CN106879471A (zh) * | 2017-04-10 | 2017-06-23 | 云南天泉生物科技股份有限公司 | 一种改良的白及组培苗生产方法 |
-
2018
- 2018-05-23 CN CN201810502968.2A patent/CN108739380B/zh active Active
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH05148115A (ja) * | 1991-07-08 | 1993-06-15 | Sanko Kk | ランの簡便な播種・発芽方法及び移植方法 |
CN103299903A (zh) * | 2013-05-23 | 2013-09-18 | 广西壮族自治区中国科学院广西植物研究所 | 一种以假鳞茎诱导假鳞茎的白芨高效繁殖方法 |
KR20150103879A (ko) * | 2014-03-04 | 2015-09-14 | 고려대학교 산학협력단 | 난 유묘의 생산방법 |
CN106879471A (zh) * | 2017-04-10 | 2017-06-23 | 云南天泉生物科技股份有限公司 | 一种改良的白及组培苗生产方法 |
Non-Patent Citations (4)
Title |
---|
LI MEIYA等: "Induction and Characterization of Tetraploids from Seeds of Bletilla striata(Thunb.) Reichb.f", 《BIOMED RESEARCH INTERNATIONAL》 * |
周勇等: "一叶兰的组织培养和快速繁殖", 《科协论坛(下半月)》 * |
崔凤芝等: "CPPU类细胞***素类物质的合成及在农业上的应用", 《河北农业技术师范学院学报》 * |
袁宁等: "白芨组培快繁育苗技术研究", 《西南农业学报》 * |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109964797A (zh) * | 2019-03-22 | 2019-07-05 | 浙江大学 | 促进白及假鳞茎增殖/膨大的方法 |
CN112154919A (zh) * | 2020-11-09 | 2021-01-01 | 广西壮族自治区农业科学院 | 一种诱导七叶一枝花愈伤组织直接成苗的培养基及方法 |
CN115644058A (zh) * | 2022-09-23 | 2023-01-31 | 广西壮族自治区农业科学院 | 一种促进兰科植物种子快速成苗的方法 |
CN115644058B (zh) * | 2022-09-23 | 2023-11-21 | 广西壮族自治区农业科学院 | 一种促进兰科植物种子快速成苗的方法 |
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CN108739380B (zh) | 2020-09-15 |
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Inventor after: Shi Yunping Inventor after: Xu Juan Inventor after: Su Zuxiang Inventor after: Li Xiaoquan Inventor after: Lin Qian Inventor after: Gui Jie Inventor after: Hu Yifeng Inventor before: Shi Yunping Inventor before: Xu Juan Inventor before: Su Zuxiang Inventor before: Li Xiaoquan Inventor before: Lin Qian Inventor before: Gui Jie Inventor before: Hu Yifeng |
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Effective date of registration: 20210517 Address after: No.174, Daxue East Road, Nanning, Guangxi 530003 Patentee after: GUANGXI ACADEMY OF AGRICULTURAL SCIENCES Address before: 530007, 174 East University Road, the Guangxi Zhuang Autonomous Region, Nanning Patentee before: BIOTECHNOLOGY Research Institute GUANGXI ACADEMY OF AGRICULTURAL SCIENCES |
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Application publication date: 20181106 Assignee: Guangxi Nanning Nongxinda Agricultural Technology Co.,Ltd. Assignor: GUANGXI ACADEMY OF AGRICULTURAL SCIENCES Contract record no.: X2023980046041 Denomination of invention: A method for one-time seedling formation of tissue culture seedlings of Baiji Granted publication date: 20200915 License type: Common License Record date: 20231108 |
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