CN108614112A - A kind of colloidal gold method half-quantitative detection human TfR CD71 detection kits - Google Patents
A kind of colloidal gold method half-quantitative detection human TfR CD71 detection kits Download PDFInfo
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- G01N33/577—Immunoassay; Biospecific binding assay; Materials therefor involving monoclonal antibodies binding reaction mechanisms characterised by the use of monoclonal antibodies; monoclonal antibodies per se are classified with their corresponding antigens
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Abstract
The invention discloses a kind of colloidal gold method half-quantitative detection human TfR CD71 detection kits comprising：Bottom plate, blotting paper, NC films, bonding pad and sample pad, the NC films are located on the bottom plate, a part for the bonding pad fits in one end of the NC films, the sample pad is located at the bonding pad top, the blotting paper part is bonded with the other end of the NC films, the CD71 monoclonal antibodies of colloid gold label are coated on the bonding pad, it is respectively equipped with detection line and nature controlling line on the NC films, it is coated with ABC71 monoclonal antibodies in the detection line, sheep anti-mouse igg is coated on the nature controlling line.The present invention uses double antibodies sandwich lateral chromatography method, by being used as capture antibody for the ABC71 monoclonal antibodies of human TfR, using colloidal gold as labeled vector, realize it is simple, quick, accurately in half-quantitative detection human body fluid TfR content purpose.
Description
Technical field
The present invention relates to immunochromatographic method biotechnology detection technique fields, and in particular to a kind of colloidal gold method sxemiquantitative inspection
Survey human TfR CD71 detection kits.
Background technology
TfR (TfR) is cell membrane transporter albumen, universally present on the various kinds of cell after birth of people, such as tire
Disk wool trait syncytial cell, the normoblast for synthesizing Hb and active tumour cell etc..TfR points are 2 kinds of hypotypes, i.e.,
TfR1 and TfR2.TfR1 is that iron absorbs and can adjust the relevant II types transmembrane protein of cell growth in a kind of mediated cell, by complete
2 articles of exactly the same monomers are formed by connecting by two articles of disulfide bond that the cystine residue on the 89th and 92 is formed.Every monomer
Containing 780 amino acid residues, molecular weight 90kD is divided into 3 regions, and 61 amino acid residues of N-terminal are in intracellular, C-terminal
It is transmembrane region that 671 amino acid residues, which are in 28 extracellular, intermediate amino aminos,.Its C-terminal region is ectodomain, packet
It is the important side that cell obtains ferro element with the TF intracellular transports mediated containing 3 N glycosylation sites and 1 O glycosylation site
Formula.
TfR2 is then mainly expressed in liver, and 80 amino acid of N-terminal form intracellular region, and 24 amino acid form transmembrane region, C
696 amino acid residues at end form extracellular region.Its extracellular region is consistent with TfR1 amino acid sequences 45%, and 66% is homologous.
Practical measurement is soluble T fR, i.e. sTfR in human body fluid.STfR molecular weight 75kD are by TfR1 extracellular regions
100-101 amino acid between peptide bond break to form, combined with Tf after exocytosis, with TfR1 keep certain proportion, mainly
It is distributed in the blood of people, (such as urine, excrement) also contains a small amount of in secretion (such as saliva) and excreta.
Soluble transferrin receptor sTfR is currently used primarily in the judgement of people's iron deficiency disease, content number directly
The shortage or surplus for reflecting iron content in human body are that hypoferric anemia or other chronic diseases cause for distinguishing anaemia patient
Anaemia and human body in the evaluation of RBC acceptor garland rate have certain reference significance.
Also, the generation of cancer cell is often accompanied with TfR and largely expresses, therefore the content detection of sTfR can be certain cancers
The early diagnosis of disease provides auxiliary foundation.At present through clinical research, nasopharyngeal carcinoma, liver cancer, hematological system tumor and breast cancer, stomach
Most of malignant tumor patients such as cancer, carcinoma of urinary bladder are all accompanied by the great expression of TfR, and content is directly predictive of tumour cell
Proliferation activity.Since there are substantial connections with tumour cell for TfR, it is used for the auxiliary diagnosis of cancer and work at present
Treatment for carrier for cancer becomes research hotspot.
The kit for being used for measuring sTfR contents on the market at present is not within minority, but is generally confined to Enzyme-linked Immunosorbent Assay
Method, immunoturbidimetry etc..Though enzyme-linked immunization has preferable linear and sensitivity, operating process is loaded down with trivial details, entire detection process
Also there is minimum requirements in the time at least needing 4 hours or more to detection sample size；Immunoturbidimetry rule confrontation original and marker
The molecular weight and quantity of formation have certain requirement, otherwise compare and are difficult to measure.
It can be seen that traditional detection method detection time is long, operating process is complicated, and the accuracy of detection is low, it would be highly desirable into
One step is improved.
Invention content
The purpose of the present invention is to provide a kind of colloidal gold method half-quantitative detection human TfR CD71 detection reagents
Box, long to solve the existing detection kit operating time, operating process is complicated, the low defect of accuracy in detection.
To achieve the above object, the present invention provides a kind of colloidal gold method half-quantitative detection human TfR CD71 detections
Kit comprising：Bottom plate, blotting paper, NC films, bonding pad and sample pad, the NC films are located on the bottom plate, the knot
The part for closing pad fits in one end of the NC films, and the sample pad is located at the bonding pad top, the blotting paper one
Divide and be bonded with the other end of the NC films, the CD71 monoclonal antibodies of colloid gold label, the NC are coated on the bonding pad
It is respectively equipped with detection line and nature controlling line on film, ABC71 monoclonal antibodies are coated in the detection line, are coated on the nature controlling line
There is sheep anti-mouse igg.
Preferably, the colloid gold particle size is 30 ± 10nm.
Preferably, the colloidal gold of the CD71 monoclonal antibodies is in conjunction with a concentration of 45-55ug/ml, optimum mark pH
8.0。
Preferably, the peridium concentration of the ABC71 monoclonal antibodies is 1-4ug/cm.
Preferably, the peridium concentration of the goat anti-mouse IgG is 1ug/cm.
Preferably, the detection kit further includes colorimetric card, and corresponding detection line is shown on the colorimetric card and is shown
The weight of color, the ranging from 0.5-8mg/L containing CD71 antibody concentrations in corresponding detection sample.
Preferably, the colloidal gold is prepared by the following method：Measure 0.01% aqueous solution of chloraurate 100ml stirrings
Heating is boiled, and is then rapidly added three sodium solution of citric acid of the 1% of 2ml, is persistently boiled 5-10min, until solution becomes orange
It is red.
Preferably, the CD71 monoclonal antibodies of the colloid gold label are made by the following method：
Colloidal gold solution is taken, pH=8.0 is adjusted with the solution of potassium carbonate of 0.2M, according to antibody：Colloidal gold=50ug：1ml
Ratio be added CD71 monoclonal antibodies, 20min is stirred at room temperature, 10% colloidal gold solution is then added to final concentration 0.5%, stirs
10min is mixed, centrifugation goes supernatant, precipitation to redissolve liquid using same volume and redissolve.
Preferably, the ingredient for redissolving liquid redissolution liquid is 50mM PB buffer solutions, pH8.0,0.5%BSA, 5% sugarcane
Sugar, 2% tween, 0.05% Sodium azide.
The invention has the advantages that：
The present invention uses double antibodies sandwich lateral chromatography method, passes through the ABC71 monoclonal antibodies for human TfR
As capture antibody, using colloidal gold as labeled vector, simple, quick, accurately half-quantitative detection human body fluid transfer iron is realized
The purpose of protein receptor content.The CD71 contents in human serum or other samples can semi-quantitatively be detected.The detection of the present invention
Reagent cartridge configuration is simple, quickly and accurately in semiquantitative determination human body fluid TfR content based on colloid gold immune
The detection kit of chromatographic technique.It is with high sensitivity, the good advantage of specificity.
Description of the drawings
The structural schematic diagram of Fig. 1 colloidal gold method half-quantitative detection human TfR CD71 detection kits of the present invention.
The standard color comparison card of Fig. 2 colloidal gold method half-quantitative detection human TfR CD71 detection kits of the present invention.
Specific implementation mode
The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention..
Embodiment 1
As shown in Figure 1, the colloidal gold method half-quantitative detection human TfR CD71 detection kits of the present invention, this examination
The detection method that agent box uses captures antibody for double-antibody method, by ABC71 mouse monoclonals, and the CD71 mouse of commercialization is single
Anti- to be used as detection antibody, sheep anti-mouse igg is as Quality Control antibody.It includes：PVC bottom plates 500, NC films 300, combine blotting paper 400
Pad 200 and sample pad 100, NC films 300 are located on bottom plate 500, and a part for bonding pad 200 fits in one end of NC films 500,
Sample pad 100 is located at 200 top of bonding pad, and 400 part of blotting paper is bonded with the other end of NC films 300, is wrapped on bonding pad 200
Had a CD71 monoclonal antibodies of colloid gold label, bonding pad 200 by colloid gold label CD71 monoclonal antibodies (Abcam,
Ab1086) uniformly polyester film material is sprayed on to dry；It is respectively equipped with detection line 310 and nature controlling line 320 on NC films 300, detects
ABC71 monoclonal antibodies are coated on line 310, the peridium concentration of ABC71 monoclonal antibodies is 1-4ug/cm.On nature controlling line 320
It is coated with sheep anti-mouse igg, the peridium concentration of goat anti-mouse igg is 1ug/cm, and detection line 310 is located at one close to bonding pad 200
End, nature controlling line 320 are located at close to one end of water absorption pad 400.The detection kit combination ABC71 monoclonal antibody conducts of the present invention
Capture antibody, have it is good specificity and higher sensitivity, have the advantages that easily and fast, it is economical and practical.
Wherein, sample pad 100 is carried out using the mixed solution of borate buffer solution, tween, bovine serum albumin(BSA) at immersion
Reason, to increase the stability and precision of kit.The detection sample of the detection kit of the present invention uses serum or urine, can
Direct sample detection.Saliva or excrement etc. can also be used, but need to be diluted the pre- places such as centrifugation using phosphate buffer before loading
Manage step, dilution ratio 1:2-1:5.Sample pad 100 is to cut out glass fibre to suitable dimension, is soaked using sample pad treatment fluid
It is taken out after bubble 5min, 37 DEG C of thermostatic drying chambers use after drying overnight.
Bonding pad 200 is that the CD71 of colloid gold label is diluted 2-5 times using liquid is redissolved, and 3-5ul/cm is equably sprayed on poly-
Ester film, 37 DEG C of thermostatic drying chambers dry formation in 2-4 hours.The coating of NC films：NC films are labelled to the specified region of PVC bottom plates,
Then ABC71 monoclonal antibodies, goat anti-mouse igg are all made of PBS and are diluted to 2mg/ml and 1mg/ml, using 1.0l/cm's
It draws film speed to draw in 320 corresponding region of detection line 310 and nature controlling line, the two interval 5mm, 37 DEG C of thermostatic drying chambers dry overnight
It is dry.The detection kit of the present invention conforms to the bonding pad 200, blotting paper 400, sample pad 100 that above-mentioned preparation is completed respectively
PVC bottom plates 500 specify region, and the test strips of wide 4mm are cut into using cutting machine, are filled to specify and get stuck, case pressing machine flattens, and is filled to and contains
There is the aluminium foil bag of drier spare.
As shown in Fig. 2, the colloidal gold semi-quantitative detection kit of the present invention further includes colorimetric card, shown on colorimetric card pair
The weight for the detection line display color answered contains CD71 antibody, concentration range 0.5- in corresponding detection sample
8mg/L。
Wherein, colloidal gold of the invention is prepared by the following method：The aqueous solution of chloraurate 100ml of measurement 0.01% is stirred
It mixes heating to boil, is then rapidly added three sodium solution of citric acid of the 1% of 2ml, persistently boils 5-10min, until solution becomes
Orange red, colloid gold particle size at this time is 30nm ± 10nm.The preparation process of the CD71 monoclonal antibodies of colloid gold label
For：The colloidal gold solution for taking above-mentioned preparation adjusts pH=8.0, according to CD71 monoclonal antibodies with the solution of potassium carbonate of 0.2M：Glue
Body gold=(45-55) ug：CD71 monoclonal antibodies are added in the ratio of 1ml, and 20min is stirred at room temperature, 10% colloidal gold is then added
Solution stirs 10min to final concentration 0.5%, and centrifugation goes supernatant, precipitation to redissolve liquid using same volume and redissolve.Wherein, liquid is redissolved
Ingredient is 50mM PB buffer solutions, pH8.0,0.5%BSA, 5% sucrose, 2% tween and 0.05% Sodium azide.
Implement the preparation and purification of the ABC71 monoclonal antibodies of 2 present invention
The detailed patent application text for preparing information and being published in the patent No. 2017106928722 of ABC71 monoclonal antibodies
In part.
(1) preparation of hybridoma
1) animal immune and cell culture：Total protein is extracted after human bladder carcinoma tissue is homogenized, and (the purchase of Balb/C mouse is immunized
From Beijing Vital River Experimental Animals Technology Co., Ltd.), carry out peritoneal immunity by the dosage of every mouse 50ug total protein.Often
Mouse is immunized again every two weeks.Mice serum potency reaches after requirement that booster immunization is primary again, takes mouse spleen to prepare after 3 days
Splenoblast suspension, is ready for cell fusion.Recovery murine myeloma cell Sp2/0 (ATCC CRL-1772), is used in combination 8-AG
(8 azaguanine) is screened to maintain sensibility of the cell to HAT.
2) cell fusion：The splenocyte suspension that step 1) prepares is merged with myeloma cell, specific method ginseng
According to《Fine works immunological experiment guide》((U.S.) J.E. science root (U.S.) D.H. Margules etc., Science Press, 2009
January publishes).Cell suspension after fusion is added to contain in feeder cells culture medium and is cultivated.After 24 hours, add HAT selection cultures
Base (is purchased from Sigma companies；HAT, that is, H:Hypoxanthine hypoxanthine, A:Aminopterin methopterins, T:
Thymidine thymidines) carry out selective culture.
3) antibody test：The hybridoma cell strain of secretory antibody is determined by ELISA method.Specific method is：Extract wing
Overnight with 4 DEG C of coatings of 0.05mol/L carbonate buffer solutions (pH9.6) 5% bovine serum albumin(BSA) is added in Guang cancerous tissue total protein
(BSA) it closes 3 hours for 37 DEG C.PBST is washed 3 times, then adds 100ul supernatants to be checked, 37 DEG C of incubation 1h.Washing 3 times, adds
Enter the anti-mouse secondary antibody IgG-HRP (be purchased from Beijing CoWin Bioscience Co., Ltd.) of horseradish peroxidase-labeled, 37
DEG C be incubated 1h.Washing 3 times adds after 50ul TMB (being purchased from Beijing Bioisystech Co., Ltd of Zhong Shan Golden Bridge) colour developings 5min is added
Enter 50ul terminate liquids.The OD values that wavelength is 450nm are read with microplate reader.OD values are more than regarding for the 2 times or more of negative control OD value
For the positive.
4) it the cloning of hybridoma and freezes：The positive hybridoma cell filtered out is cloned using limiting dilution assay
Change culture.By the colonized culture of 5 wheels, the hybridoma for filtering out high-titer monoclonal antibody is enlarged culture.
A kind of positive hybridoma cell strain obtained in the present invention is the monoclonal hybridoma strain of antihuman bladder cancer, should
Hybridoma cell strain was preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center on July 27th, 2017
(CGMCC, China, Beijing), preserving number CGMCCNo.14312.
(2) preparation and purification of ABC71 monoclonal antibodies
The hybridoma cell strain (preserving number CGMCCNo.14312) of above-mentioned secretion ABC71 monoclonal antibodies is expanded into culture,
And collect cells and supernatant.Affinitive layer purification is carried out to ABC71 monoclonal antibodies using Protein G.Step is：First
With phosphate buffer PBS balance Protein G affinity columns (being purchased from GE companies)；Then it will contain ABC71 monoclonal antibodies
Cells and supernatant pass through Protein G affinity columns；Chromatographic column then is washed with PBS, the washing until flowing out pillar
The OD values of liquid are close to zero；Protein G affinity columns are eluted using the glycine-HCL solution (PH2.8) of 0.2mol/L, are received
Collect eluent, measures OD values.The eluent of the monoclonal antibody containing ABC71 freezes after PBS dialyses in -20 DEG C.
The identification of ABC71 monoclonal antibodies in the present invention：
Human bladder carcinoma tissue and Human normal bladder histotomy are carried out using ABC71 monoclonal antibodies prepared by the present invention
Immunohistochemistry.The result shows that positive reaction is presented after ABC71 monoclonal antibody immunity histochemical stainings in human bladder carcinoma tissue, and
Negative reaction is presented after ABC71 monoclonal antibody immunity histochemical stainings in Human normal bladder tissue.ABC71 prepared by the present invention is mono-
Clonal antibody and human bladder carcinoma tissue are in strong positive reaction, and with Human normal bladder tissue no cross reaction.
1 immunohistochemistry of table detects antihuman bladder cancer ABC71 monoclonal antibodies to human bladder carcinoma tissue and normal bladder tissue
Immune response
| It organizes (cancerous tissue and normal structure) | ABC71 antibody |
| Human bladder carcinoma tissue (patient #1) | Strong positive (+++) |
| Human normal bladder tissue (patient #1) | Negative (-) |
| Human bladder carcinoma tissue (patient #2) | Strong positive (+++) |
| Human normal bladder tissue (patient #2) | Negative (-) |
| Human bladder carcinoma tissue (patient #3) | Strong positive (+++) |
| Human normal bladder tissue (patient #3) | Negative (-) |
| Human bladder carcinoma tissue (patient #4) | Positive (++) |
| Human normal bladder tissue (patient #4) | Negative (-) |
| Human bladder carcinoma tissue (patient #5) | Strong positive (+++) |
| Human normal bladder tissue (patient #5) | Negative (-) |
The detection kit of 3 colloidal gold immunity chromatography half-quantitative detection human TfR CD71 of the present invention of embodiment
Methodology index
1, the sensitivity of colloidal gold immunity chromatography half-quantitative detection kit of the present invention
A volunteers sera's sample is taken, using the Human sTfR Quantikine IVD ELISA of R＆D companies of the U.S.
It is 20.8nmol/L, i.e. 1.52mg/L that Kit, which measures its CD71 content,.We are diluted to 1.5mg/ using phosphate buffer
L, 1.0mg/L, 0.5mg/L, 0.1mg/L loading are tested, as a result, it has been found that T lines show that human eye is rigid when sample concentration is in 0.5mg/L
The micro- red line that can be distinguished, T lines are blank when sample concentration 0.1mg/L, therefore the detection sensitivity of this detection reagent card is not higher than
0.5mg/L。
2, the interpretation of detection kit result of the present invention
A volunteers sera's sample, the present invention is taken to use the Human sTfR Quantikine IVD of R＆D companies of the U.S.
The content that ELISA Kit measure its CD71 monoclonal antibody is 20.8nmol/L, i.e. 1.52mg/L.By adding blank phosphate
Buffer solution dilutes or addition CD71 recombinant antigens, and it is respectively 1mg/L, 2mg/L, 3mg/L, 4mg/L, 5mg/ to be configured to CD71 concentration
L, the sample of 6mg/L, 7mg/L, 8mg/L carry out loading, as shown in Figure 2：When concentration is less than 3mg/L, T line color depth is less than C
Line color depth；When concentration is equal to 4mg/L, T line colors depth and C line color depth are almost consistent；When concentration is more than 4mg/L, T
Line color depth is more than C lines；But T line colors depth is slightly shallower than 7mg/L when concentration 8mg/L, illustrates that CD71 concentration is opened in 8mg/L
There are hook effects in beginning.It is prepared for a color colorimetric card according to the shade of various concentration T line outlets, by comparing T lines
The shade of outlet can intuitively substantially in judgement sample CD71 content.
3, the accuracy of colloidal gold semi-quantitative detection kit of the present invention
The present invention acquires 100 parts of different human serum samples, using the Human sTfR of R＆D companies of the U.S.
Quantikine IVD ELISA Kit are quantified, and the sample for measuring concentration in 1-2mg/L is 38 parts, in the sample of 2-3mg/L
This is 46 parts, and sample has 16 parts in the sample of 3-4mg/L.It is concentration in 1- to use the result that detection kit of the present invention measures
The sample of 2mg/L is 40 parts, is 45 parts in the sample of 2-3mg/L, and sample has 15 parts in the sample of 3-4mg/L, specific such as table 1：
The detection kit accuracy statistical result of 1 present invention of table
Provide that the control of this two CV values is tolerance interval within 5%.This hair is can be seen that by the result in table
The accuracy of bright detection kit meets the requirements.
The precision of the detection kit of the present invention, takes 1 part of volunteers sera's sample, using the Human of R＆D companies of the U.S.
It is 19.6nmol/L, i.e. 1.43mg/L that sTfR Quantikine IVD ELISA Kit, which measure its CD71 content,.With reference to embodiment
The sample processing method of " interpretations of kit results of the present invention " in 1, prepare concentration be respectively 1.5mg/ml, 3.5mg/ml,
The sample of 6.5mg/ml, each concentration repeat detection 10 times to the detection reagent card of 3 batches respectively, compare colorimetric card, core
To coincidence rate, as a result such as table 2：
2 kit of the present invention of table criticizes interior, poor statistical result
The present invention shows that three batch detection reagent card withinrun precisions and betweenrun precision are good by test result,
Meet test request.
5, the stability of detection kit of the invention
The detection kit of the present invention is packaged in the aluminium foil bag equipped with drier, is taken with a batch of detection reagent card
It is respectively placed in 37 DEG C of constant temperature and humidity incubators and 4 DEG C of refrigerators and continuously places, meanwhile, it separately takes a part to be placed in 25 DEG C of room temperatures and puts
Set, it is primary every detection in 7 days using the serum sample of CD71 contents 3mg/L as quality-control product, extreme case with connect under room temperature
Continuous detection 12 months.It is learnt by statistical result, is positioned over and is placed at room temperature under detection kit extreme condition of the invention, effectively
Phase is at 12 months or more.
Although above having used general explanation and specific embodiment, the present invention is described in detail, at this
On the basis of invention, it can be made some modifications or improvements, this will be apparent to those skilled in the art.Therefore,
These modifications or improvements without departing from theon the basis of the spirit of the present invention belong to the scope of protection of present invention.
Claims (9)
1. a kind of colloidal gold method half-quantitative detection human TfR CD71 detection kits, which is characterized in that the detection
Kit includes：Bottom plate, blotting paper, NC films, bonding pad and sample pad, the NC films are located on the bottom plate, the combination
A part for pad fits in one end of the NC films, and the sample pad is located at the bonding pad top, the blotting paper part
It is bonded with the other end of the NC films, the CD71 monoclonal antibodies of colloid gold label, the NC films is coated on the bonding pad
On be respectively equipped with detection line and nature controlling line, ABC71 monoclonal antibodies are coated in the detection line, are coated on the nature controlling line
Sheep anti-mouse igg.
2. detection kit as described in claim 1, which is characterized in that
The colloid gold particle size is 30 ± 10nm.
3. detection kit as described in claim 1, which is characterized in that
It is 8.0 that the colloidal gold of the CD71 monoclonal antibodies, which combines a concentration of 45-55ug/ml, optimum mark pH,.
4. detection kit as described in claim 1, which is characterized in that
The peridium concentration of the ABC71 monoclonal antibodies is 1-4ug/cm.
5. detection kit as described in claim 1, which is characterized in that
The peridium concentration of the goat anti-mouse IgG is 1ug/cm.
6. detection kit as described in claim 1, which is characterized in that
The detection kit further includes colorimetric card, and the depth of corresponding detection line display color is shown on the colorimetric card
It spends, the ranging from 0.5-8mg/L containing CD71 antibody concentrations in corresponding detection sample.
7. detection kit as described in claim 1, which is characterized in that
The colloidal gold is prepared by the following method：The aqueous solution of chloraurate 100ml agitating and heatings for measuring 0.01% are boiled, so
It is rapidly added three sodium solution of citric acid of the 1% of 2ml afterwards, persistently boils 5-10min, until solution becomes orange red.
8. detection kit as described in claim 1, which is characterized in that
What the CD71 monoclonal antibodies of the colloid gold label were made by the following method：
Colloidal gold solution is taken, pH=8.0 is adjusted with the solution of potassium carbonate of 0.2M, according to antibody：Colloidal gold=50ug：The ratio of 1ml
CD71 monoclonal antibodies are added in example, and 20min is stirred at room temperature, and 10% colloidal gold solution is then added to final concentration 0.5%, stirring
10min, centrifugation go supernatant, precipitation to redissolve liquid using same volume and redissolve.
9. detection kit as described in claim 1, which is characterized in that
It is described redissolve liquid redissolve liquid ingredient be 50mM PB buffer solutions, pH8.0,0.5%BSA, 5% sucrose, 2% tween,
0.05% Sodium azide.
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| CN108026170A (en) * | 2015-05-04 | 2018-05-11 | 西托姆克斯治疗公司 | Anti- CD71 antibody, can activate anti-CD71 antibody and its application method |
| CN107573414A (en) * | 2017-08-14 | 2018-01-12 | 李翀 | Human bladder cancer mark AG CD71 and its antibody A BC71 and application |
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