CN108077651A - A kind of spotted maigre young postlarva micro-encapsulated diet and preparation method thereof - Google Patents
A kind of spotted maigre young postlarva micro-encapsulated diet and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a kind of spotted maigre young postlarva micro-encapsulated diet and preparation method thereof, the spotted maigre young postlarva micro-encapsulated diet, including the following raw material：White fish meal, krill meal, squid powder, fish protein hydrolysate levels, fodder yeast, α starch, soybean lecithin, papain, fish oil, multi-vitamins, composite mineral matter, binding agent, immunopotentiator, phagostimulant, medium chain triglyceride, decanoylacetaldehyde sodium hydrosulfite, 5 aminosalicylic acids；Wherein described binding agent is sodium alginate, and the immunopotentiator is glucan and chitosan, and the phagostimulant is taurine, the spotted maigre young postlarva micro-encapsulated diet be by crushing, being granulated, being coated, sub-sieve and etc. made of.The product of the present invention compared with prior art, can improve the average length and survival rate of spotted maigre young postlarva.
Description
【Technical field】
The invention belongs to agriculture mariculture technologies, and in particular to a kind of spotted maigre young postlarva micro-encapsulated diet and its preparation
Method.
【Background technology】
Spotted maigre (Nibea albiflora Richardson) is commonly called as yellow mother-in-law chicken, belongs to Perciformes, Sciaenidae, yellow aunt
Fish category, the bodily form is similar to Larimichthys crocea, belongs to lower water layer spotted maigre in warm warm nature coastal waters, mainly inhabits the shallower littoral sea of sand mud substrate
Domain using zoobenthos such as small-sized shell-fish and small fish as food, is distributed mainly on northwest Pacific area, South Sea in China, the East Sea and
The Huanghai Sea has its distribution, and 8-33 DEG C of suitable temperature range, proper salinity level 10-40 ‰, the most suitable growth water temperature is 24-29 DEG C, water temperature of laying eggs
24-28 DEG C, age at sexual maturity is 2 week old.Spotted maigre is the economic spotted maigre of the important seawater in China, has that meat flavour is delicious, nutrition
Abundant, the features such as culture benefit is high.At present, carried out the extensive of spotted maigre in the Fujian in China and Zhejiang area manually to educate
Seedling, but the special compound feed of spotted maigre young postlarva is not yet researched and developed, and is cultivated feed and is mainly used wheel animalcule, artemia and Copepods etc.,
Using existing marine fish larvae feedstuff feeding spotted maigre, there are not comprehensive nutrition, the series such as palatability is poor, absorption rate is low
Problem has seriously affected the survival rate of spotted maigre, constrains the further development of its aquaculture industry.Therefore, spotted maigre young is carried out
The development of the special micro-encapsulated diet of postlarva, to improving spotted maigre weed survival rate, solving spotted maigre propagation in scale, the yellow aunt of quickening
Fish culture industrialization process all has positive facilitation.
" a kind of spotted maigre young postlarva micro-encapsulated diet and its processing are prepared and (authorized public with feeding method Chinese patent literature
Announcement number：CN102845653B) " disclose a kind of spotted maigre young postlarva micro-encapsulated diet and its processing prepares and feeding method, it
It is made of the raw material of following weight percent：White fish meal 30%-50%, krill meal 10%-20%, squid powder 5%-
15%th, fish protein hydrolysate levels 2%-8%, fodder yeast 3%-9%, alphalise starch 7.5%-8.5%, soybean lecithin 2%-8%,
Papain 1%-3%, fish oil 1%-5%, multi-vitamins 1%-3%, composite mineral matter 1%-3%, binding agent
0.5%-1.5%, immunopotentiator 0.05%-0.15%, phagostimulant 0.5%-1.5%.The invention can meet larva and juvenile life
Long nutritional need, and have many advantages, such as that nutrition is comprehensive, palatability is good, water stability energy is good, it can manufacture, convenient for storage
It hides, transport and feeds, be suitble to the Seedling production of batch production, while employ corresponding feeding method, spotted maigre young can be improved
The growth of postlarva and survival rate are suitble to large-scale promotion to use, but also have greatly improved in terms of its average length and survival rate
Space.
【The content of the invention】
The present invention provides a kind of spotted maigre young postlarva micro-encapsulated diet and preparation method thereof, to solve in Chinese patent literature
" a kind of spotted maigre young postlarva micro-encapsulated diet and its processing are prepared and feeding method (Authorization Notice No.：CN102845653B it is) " public
On the basis of the formula opened, optimize component, dosage, method etc., further improve the average length and survival rate of spotted maigre young postlarva
The technical issues of.
For solution more than technical problem, the present invention uses following technical scheme：
A kind of spotted maigre young postlarva micro-encapsulated diet, which is characterized in that including the following raw material：White fish meal, krill meal, squid
Powder, fish protein hydrolysate levels, fodder yeast, alphalise starch, soybean lecithin, papain, fish oil, multi-vitamins, grandidierite
Substance, binding agent, immunopotentiator, phagostimulant, medium chain triglyceride, decanoylacetaldehyde sodium hydrosulfite, 5-aminosalicylic acid；Its
Described in binding agent be sodium alginate, the immunopotentiator be glucan and chitosan, the phagostimulant be taurine；It is described
Medium chain triglyceride, decanoylacetaldehyde sodium hydrosulfite, the mass ratio of 5-aminosalicylic acid are (0.5-0.1):(0.4-0.8):
(0.1-0.3)。
Further, the medium chain triglyceride, decanoylacetaldehyde sodium hydrosulfite, the mass ratio of 5-aminosalicylic acid are
0.8:0.6:0.2。
Further, the spotted maigre young postlarva micro-encapsulated diet, which is characterized in that in units of mass parts, including
The following raw material：White fish meal 35-45 mass parts, krill meal 12-18 mass parts, squid powder 8-12 mass parts, fish protein hydrolysate levels 4-6
Mass parts, fodder yeast 5-7 mass parts, alphalise starch 7.8-8 mass parts, soybean lecithin 4-6 mass parts, papain 1.5-
2.5 mass parts, fish oil 2-4 mass parts, multi-vitamins 1.5-2.5 mass parts, composite mineral matter 1.5-2.5 mass parts, bonding
Agent 0.8-1.2 mass parts, immunopotentiator 0.08-0.12 mass parts, phagostimulant 0.8-1.2 mass parts, medium chain triglyceride
0.5-0.1 mass parts, decanoylacetaldehyde sodium hydrosulfite 0.4-0.8 mass parts, 5-aminosalicylic acid 0.1-0.3 mass parts.
Further, the spotted maigre young postlarva micro-encapsulated diet, which is characterized in that in units of mass parts, including
The following raw material：40 mass parts of white fish meal, 15 mass parts of krill meal, 10 mass parts of squid powder, 5 mass parts of fish protein hydrolysate levels, feeding
Expect 6 mass parts of yeast, 7.9 mass parts of alphalise starch, 5 mass parts of soybean lecithin, 2 mass parts of papain, 3 mass of fish oil
Part, 2 mass parts of multi-vitamins, 2 mass parts of composite mineral matter, 1 mass parts of binding agent, 0.1 mass parts of immunopotentiator, food calling
1 mass parts of agent, 0.8 mass parts of medium chain triglyceride, 0.6 mass parts of decanoylacetaldehyde sodium hydrosulfite, 0.2 matter of 5-aminosalicylic acid
Measure part.
Further, glucan is added with chitosan equal proportion in the profit immunopotentiator.
The present invention also provides a kind of preparation methods of above-mentioned spotted maigre young postlarva micro-encapsulated diet, which is characterized in that bag
Include following steps：
1) solid feed raw material is crushed with micronizer；
2) all feeds raw material in the ratio of feed formula is weighed, mixed, be first sufficiently mixed by hand, pass through 100 mesh
Then sieve is mixed with mixer；
3) particle is made in mixed raw material；
4) manufactured particle feed using fluid bed bottom is sprayed or is coated with sugar coating machine, coating solution is uniformly sprayed
In feed surface, micro-encapsulated diet is made, wherein coating solution is using the aqueous solution of gelatin, the ethanol solution of ethyl cellulose, jade
One kind in the rice ethanol solution of alcohol soluble protein, the ethanol solution of resin；
5) micro-encapsulated diet of preparation is subjected to grain size grading by the shaking screen of different meshes with sieve method；
6) feed being classified is sealed in vacuum packing machine in aluminium foil bag, be stored in 4 DEG C of refrigerator for use.
Further, particle is made using one of following 3 kinds of methods in step 3)：A. by mixed raw material using fluidisation
Particle is made in bed top spray method, includes the following steps：Mixed material is placed in fluid bed, peristaltic pump sprays into water, flow
For 0.5-1.5mL/min, the inlet air temperature of fluid bed is 60-80 DEG C, leaving air temp is 30-50 DEG C, 40-60 DEG C of bed temperature, compression
Atmospheric pressure 20-30MPa, feed product particle diameter distribution obtained is between 150-600 μm；B. by mixed raw material using crowded
Go out-particle is made in spheronization, include the following steps：Mixed material is added in the water of core amount 5%-10%, is mixed with groove profile
Conjunction machine mixes 10-30min with the process conditions of rotating speed 20-30rpm, 100-120 ° of batch turning angle, and it is uniformly wet that moisture content is made
The moist wood prepared is placed in the stainless steel screen drum of rotating type granulating machine by material, and through rotary squeezing, cylindrical bars are formed by mesh
Shape feed, and it is aided with the method for hand-crushed into wet granular, size controlling puts into shot-blasting machine at 150-600 μm, by wet granular
Rotating centrifugal disk in rotating speed 5-15rpm throw circle 20-40min pellet is made；C. mixed raw material is used into efficient wet
Particle is made in granulator, includes the following steps：Mixed feed is put into efficient wet granulator, passes through certain mesh number
Particle is made in mesh, and the size of particle is adjusted according to the size of mesh.
Further, it is coated using the method for fluid bed bottom spray in step 4), is carried out with hot water soluble coating solution
Bottom is sprayed, and process conditions are that inlet air temperature is 50-70 DEG C, and leaving air temp is 20-40 DEG C, and bed temperature is 35-45 DEG C, atmospheric pressure 15-
25MPa, the flow velocity of peristaltic pump is 0.5-1.5mL/min.
The invention has the advantages that：
(1) by the data of embodiment 1-3 and comparative example 5 as it can be seen that spotted maigre young postlarva micro-encapsulated diet using embodiment 1
Average length and survival rate obtained comprehensive raising；Simultaneously by the data of embodiment 1-3 as it can be seen that embodiment 1 is optimal implementation
Example.
(2) by embodiment 1 and the data of comparative example 1-4 as it can be seen that medium chain triglyceride, decanoylacetaldehyde sodium hydrosulfite, 5-
Aminosalicylic acid plays synergistic effect during spotted maigre young postlarva micro-encapsulated diet is fed, and collaboration improves average length
And survival rate, this may be：
1) medium chain triglyceride is added in, the viscosity of fatty substance of high viscosity will greatly reduce, and can greatly improve food
The fuel factor of material and stomach, while can be mutual with decanoylacetaldehyde sodium hydrosulfite, 5-aminosalicylic acid to the assimilation effect of fat
With reference to being dispersed in feed and play stable effect, while in spotted maigre feeding process, can be dissociated the last of the ten Heavenly stems in stomach
The active ingredient of acyl acetaldehyde sodium hydrogensulfite, 5-aminosalicylic acid, so as to improve suction of the spotted maigre to feed and its active ingredient
It receives, so as to increase absorption efficiency, therefore adds the survival rate of spotted maigre and the average length of spotted maigre.
2) 5-aminosalicylic acid has significant antiinflammation to intestinal wall inflammation, due to having amino, hydroxyl and carboxyl on ring
Three active reactive groups, can be combined with each other with decanoylacetaldehyde sodium hydrosulfite, play the role of collaboration and promote anti-inflammatory,
It both can also promote metabolism in vivo simultaneously, while coordinate medium chain triglyceride as sustained release agent, the effect of sustained release be played, so as to increase
Add it in the spotted maigre in vivo profit reduction and reserving time, so as to add the average length of the survival rate of spotted maigre and spotted maigre.
3) decanoylacetaldehyde sodium hydrosulfite is sodium houttuyfonate, itself has certain antibiotic property and to the agreeable to the taste of fish
Property, while there is hydrophily and lipophile, it can be combined with medium chain triglyceride, easily be distributed to the effect that sustained release is played in feed
Fruit, while can interact with 5-aminosalicylic acid under acid instruction in stomach, it is promoted to be absorbed by the cell membrane of stomach
Enter in blood, so as to promote spotted maigre and vitality, therefore add the survival rate of spotted maigre and the average length of spotted maigre.
(3) medium chain triglyceride of the invention, decanoylacetaldehyde sodium hydrosulfite, 5-aminosalicylic acid be as reinforcement system,
By control medium chain triglyceride, decanoylacetaldehyde sodium hydrosulfite, 5-aminosalicylic acid mass ratio be (0.5-0.1):(0.4-
0.8):(0.1-0.3) is realized in reinforcement system with medium chain triglyceride sustained release agent and stabilizer, 5-aminosalicylic acid conduct
Activating agent, while decanoylacetaldehyde sodium hydrosulfite is as sorbefacient so that reinforcement system applies to the spotted maigre of the present invention
The average length and survival rate of spotted maigre young postlarva can be effectively improved in larva and juvenile micro-encapsulated diet.
【Specific embodiment】
It for ease of more fully understanding the present invention, is illustrated by following instance, these examples belong to the protection of the present invention
Scope, but do not limit the scope of the invention.
In embodiment, white fish meal (crude protein 67%；Crude fat 9% (dry weight), seafood company of the U.S.) 35-45 mass
Part, krill meal (crude protein 63.9%；Crude fat be 5.6% (dry weight), Zhoushan Fengshun fish meal products Co., Ltd) 12-18
Mass parts, squid powder (crude protein 50.3%；Crude fat be 4.9% (dry weight), Zhoushan Marine Fishery Co., State Bureau of Aquatic Products's fish meal
Factory) 8-12 mass parts, fish protein hydrolysate levels (crude protein 64.3%, crude fat be 7.6% (dry weight), self-control, using tryptose
Enzyme and papain hydrolysis white fish meal are made) it is 4-6 mass parts, fodder yeast 5-7 mass parts, alphalise starch 7.8-8 mass parts, big
Beans lecithin 4-6 mass parts, papain 1.5-2.5 mass parts, fish oil 2-4 mass parts, multi-vitamins 1.5-2.5 mass
Part, composite mineral matter 1.5-2.5 mass parts, sodium alginate 0.8-1.2 mass parts, immunopotentiator 0.08-0.12 mass parts, ox
Sulfonic acid 0.8-1.2 mass parts, medium chain triglyceride 0.5-0.1 mass parts, decanoylacetaldehyde sodium hydrosulfite 0.4-0.8 mass parts,
5-aminosalicylic acid 0.1-0.3 mass parts；Wherein described immunopotentiator mixes for glucan and chitosan equal proportion.
The preparation method of above-mentioned spotted maigre young postlarva micro-encapsulated diet, comprises the following steps：
(1) by solid material and additive micronizer (WFJ series micronizers, gold section of Jiangyin City pulverizer
Tool Co., Ltd) it crushes, granularity is less than 20 μm；
(2) all feeds raw material is weighed in proportion, the principle mixing being superimposed in a small amount adds in a certain proportion of water, first uses
It is sufficiently mixed by hand, all by 100 mesh sieves, then with (the YH series 3 D multi-directional movement mixing of 3 D multi-directional movement mixer
Machine, Changzhou Pu Naier drying equipments Co., Ltd) mixing 10min；
(3) particle is made in mixed raw material, particle is made using one of following 3 kinds of methods：A. by mixed original
Particle is made using fluid bed top spray method in material, includes the following steps：Mixed material is placed in fluid bed, peristaltic pump spray
Enter water, flow 0.5-1.5mL/min, the inlet air temperature of fluid bed is 60-80 DEG C, leaving air temp is 30-50 DEG C, bed temperature 40-
60 DEG C, compressed air pressure 20-30MPa, feed product particle diameter distribution obtained is between 150-600 μm；It b. will be mixed
Particle is made using extrusion-spheronization in raw material, includes the following steps：Mixed material is added in core amount 5%-10%'s
Water mixes 10-30min with the process conditions of rotating speed 20-30rpm, 100-120 ° of batch turning angle with trough type mixing machine, is made aqueous
The uniform moist wood of rate, the moist wood prepared is placed in the stainless steel screen drum of rotating type granulating machine, through rotary squeezing, passes through mesh
Cylinder strip feed is formed, and is aided with the method for hand-crushed into wet granular, size controlling throws wet granular at 150-600 μm
Enter into the rotating centrifugal disk of shot-blasting machine and pellet is made with rotating speed 5-15rpm throwing circles 20-40min；C. mixed raw material is adopted
Particle is made with efficient wet granulator, includes the following steps：Mixed feed is put into efficient wet granulator, is passed through
Particle is made in the mesh of certain mesh number, and the size of particle is adjusted according to the size of mesh；
(4) manufactured particle feed using fluid bed bottom is sprayed or is coated with sugar coating machine, coating solution is uniformly sprayed
Be coated in feed surface, be made micro-encapsulated diet, wherein coating solution using the aqueous solution of gelatin, ethyl cellulose ethanol solution,
One kind in the ethanol solution of zeins, the ethanol solution of resin；It is coated, used using the method that fluid bed bottom is sprayed
Hot water soluble coating solution carries out bottom spray, and process conditions are that inlet air temperature is 50-70 DEG C, and leaving air temp is 20-40 DEG C, and bed temperature is
35-45 DEG C, atmospheric pressure 15-25MPa, the flow velocity of peristaltic pump is 0.5-1.5mL/min, single crowd of yield 2kg, the dosage of coating solution
For 100g；
(5) by the micro-encapsulated diet of preparation by 100 mesh, 80 mesh, 60 mesh, 40 mesh, 30 mesh, 20 mesh, 10 mesh shaking screen,
Grain size grading is carried out using sieve method；
(6) it is the feed being classified is for use in aluminium foil bag, being stored in 4 DEG C of refrigerator with vacuum packing machine sealing.
It is illustrated below by more specific embodiment.
Embodiment 1
A kind of spotted maigre young postlarva micro-encapsulated diet, in units of mass parts, including the following raw material：White fish meal (crude protein
For 67%；Crude fat 9% (dry weight), seafood company of the U.S.), 40 mass parts；Krill meal (crude protein 63.9%；Crude fat is
5.6% (dry weight), Zhoushan Fengshun fish meal products Co., Ltd), 15 mass parts；Squid powder (crude protein 50.3%；Crude fat
For 4.9% (dry weight), Zhoushan Marine Fishery Co., State Bureau of Aquatic Products's fish meal factory), 10 mass parts；(crude protein is fish protein hydrolysate levels
64.3%, crude fat is 7.6% (dry weight), and self-control is made of trypsase and papain hydrolysis white fish meal) 5 mass
Part；Fodder yeast, 6 mass parts；Alphalise starch, 7.9 mass parts；Soybean lecithin, 5 mass parts；Papain, 2 mass parts；Sea
Mosanom, 1 mass parts；Fish oil, 3 mass parts；Multi-vitamins, 2 mass parts；Composite mineral matter, 2 mass parts, taurine, 1 matter
Measure part；Glucan, 0.05 mass parts；Chitosan, 0.05 mass parts；0.8 mass parts of medium chain triglyceride, decanoyl acetaldehyde sulfurous acid
0.6 mass parts of hydrogen sodium, 0.2 mass parts of 5-aminosalicylic acid.
The preparation method of above-mentioned spotted maigre young postlarva micro-encapsulated diet, comprises the following steps：
(1) by solid material and additive micronizer (WFJ series micronizers, gold section of Jiangyin City pulverizer
Tool Co., Ltd) it crushes, granularity is less than 20 μm；
(2) all feeds raw material is weighed in proportion, the principle mixing being superimposed in a small amount adds in a certain proportion of water, first uses
It is sufficiently mixed by hand, all by 100 mesh sieves, then with (the YH series 3 D multi-directional movement mixing of 3 D multi-directional movement mixer
Machine, Changzhou Pu Naier drying equipments Co., Ltd) mixing 10min；
(3) particle is made using fluid bed top spray method in mixed raw material, included the following steps：By mixed object
Material is placed in fluid bed, and peristaltic pump sprays into water, and flow 1mL/min, the inlet air temperature of fluid bed is 70 DEG C, leaving air temp 40
DEG C, 50 DEG C of bed temperature, compressed air pressure 25MPa, feed product particle diameter distribution obtained is between 500 μm；
(4) manufactured particle feed using sugar coating machine is coated, coating solution is uniformly sprayed in feed surface, system
Into micro-encapsulated diet, wherein coating solution uses the aqueous solution of gelatin, and single crowd of yield 2kg, the dosage of coating solution is 100g；
It uses fluid bed bottom spray method instead, hot water soluble gelatin solution is subjected to bottom spray, process conditions are that inlet air temperature is
60 DEG C, leaving air temp is 30 DEG C, and bed temperature is 40 DEG C, atmospheric pressure 20mPa, and the flow velocity of peristaltic pump is 1mL/min, single batch of yield
2kg, coating material are gelatin solution, and the dosage of gelatin is 100g；
(5) by the micro-encapsulated diet of preparation by 100 mesh, 80 mesh, 60 mesh, 40 mesh, 30 mesh, 20 mesh, 10 mesh shaking screen,
Grain size grading is carried out using sieve method；
(6) it is the feed being classified is for use in aluminium foil bag, being stored in 4 DEG C of refrigerator with vacuum packing machine sealing.
Embodiment 2
A kind of spotted maigre young postlarva micro-encapsulated diet, in units of mass parts, including the following raw material：White fish meal (crude protein
For 67%；Crude fat 9% (dry weight), seafood company of the U.S.) 35 mass parts, krill meal (crude protein 63.9%；Crude fat is
5.6% (dry weight), Zhoushan Fengshun fish meal products Co., Ltd) 18 mass parts, squid powder (crude protein 50.3%；Crude fat
For 4.9% (dry weight), Zhoushan Marine Fishery Co., State Bureau of Aquatic Products's fish meal factory) 8 mass parts, (crude protein is fish protein hydrolysate levels
64.3%, crude fat is 7.6% (dry weight), and self-control is made of trypsase and papain hydrolysis white fish meal) 6 mass
Part, 5 mass parts of fodder yeast, 8 mass parts of alphalise starch, 4 mass parts of soybean lecithin, 2.5 mass parts of papain, fish oil 2
Mass parts, 2.5 mass parts of multi-vitamins, 1.5 mass parts of composite mineral matter, 1.2 mass parts of sodium alginate, 0.04 matter of glucan
Measure part, 0.04 mass parts of chitosan, 1.2 mass parts of taurine, 0.5 mass parts of medium chain triglyceride, decanoyl acetaldehyde bisulfite
0.8 mass parts of sodium, 0.1 mass parts of 5-aminosalicylic acid.
The preparation method of above-mentioned spotted maigre young postlarva micro-encapsulated diet, comprises the following steps：
(1) by solid material and additive micronizer (WFJ series micronizers, gold section of Jiangyin City pulverizer
Tool Co., Ltd) it crushes, granularity is less than 20 μm；
(2) all feeds raw material is weighed in proportion, the principle mixing being superimposed in a small amount adds in a certain proportion of water, first uses
It is sufficiently mixed by hand, all by 100 mesh sieves, then with (the YH series 3 D multi-directional movement mixing of 3 D multi-directional movement mixer
Machine, Changzhou Pu Naier drying equipments Co., Ltd) mixing 10min；
(3) particle is made using extrusion-spheronization in mixed raw material, included the following steps：By mixed material
The water of core amount 6% is added in, 10min, system are mixed with the process conditions of rotating speed 30rpm, 120 ° of batch turning angle with trough type mixing machine
Into the uniform moist wood of moisture content, the moist wood prepared is placed in the stainless steel screen drum of rotating type granulating machine, through rotary squeezing, is led to
It crosses mesh and forms cylindrical bars shape feed, and be aided with the method for hand-crushed into wet granular, size controlling is at 500 μm, by wet granular
It puts into the rotating centrifugal disk of shot-blasting machine and pellet is made with rotating speed 10rpm throwing circles 30min；
(4) manufactured particle feed using the spray of fluid bed bottom is coated, coating solution is uniformly sprayed in feed table
Micro-encapsulated diet is made in face, and wherein coating solution uses the ethanol solution of ethyl cellulose, is carried out using the method for fluid bed bottom spray
Coating, carries out bottom spray, process conditions are that inlet air temperature is 60 DEG C, go out wind-warm syndrome with the ethanol solution of hot water soluble ethyl cellulose
It spends for 30 DEG C, bed temperature is 40 DEG C, atmospheric pressure 20MPa, and the flow velocity of peristaltic pump is 1mL/min, single crowd of yield 2kg, ethyl cellulose
The dosage of the ethanol solution of element is 100g；
(5) by the micro-encapsulated diet of preparation by 100 mesh, 80 mesh, 60 mesh, 40 mesh, 30 mesh, 20 mesh, 10 mesh shaking screen,
Grain size grading is carried out using sieve method；
(6) it is the feed being classified is for use in aluminium foil bag, being stored in 4 DEG C of refrigerator with vacuum packing machine sealing.
Embodiment 3
A kind of spotted maigre young postlarva micro-encapsulated diet, in units of mass parts, including the following raw material：White fish meal (crude protein
For 67%；Crude fat 9% (dry weight), seafood company of the U.S.) 45 mass parts, krill meal (crude protein 63.9%；Crude fat is
5.6% (dry weight), Zhoushan Fengshun fish meal products Co., Ltd) 12 mass parts, squid powder (crude protein 50.3%；Crude fat
For 4.9% (dry weight), Zhoushan Marine Fishery Co., State Bureau of Aquatic Products's fish meal factory) 12 mass parts, (crude protein is fish protein hydrolysate levels
64.3%, crude fat is 7.6% (dry weight), and self-control is made of trypsase and papain hydrolysis white fish meal) 4 mass
Part, 7 mass parts of fodder yeast, 7.8 mass parts of alphalise starch, 6 mass parts of soybean lecithin, 1.5 mass parts of papain, fish oil
4 mass parts, 1.5 mass parts of multi-vitamins, 2.5 mass parts of composite mineral matter, 0.8 mass parts of sodium alginate, glucan 0.06
Mass parts, 0.06 mass parts of chitosan, 0.8 mass parts of taurine, 0.1 mass parts of medium chain triglyceride, decanoyl acetaldehyde sulfurous acid
0.4 mass parts of hydrogen sodium, 0.3 mass parts of 5-aminosalicylic acid.
The preparation method of above-mentioned spotted maigre young postlarva micro-encapsulated diet, comprises the following steps：
(1) by solid material and additive micronizer (WFJ series micronizers, gold section of Jiangyin City pulverizer
Tool Co., Ltd) it crushes, granularity is less than 20 μm；
(2) all feeds raw material is weighed in proportion, the principle mixing being superimposed in a small amount adds in a certain proportion of water, first uses
It is sufficiently mixed by hand, all by 100 mesh sieves, then with (the YH series 3 D multi-directional movement mixing of 3 D multi-directional movement mixer
Machine, Changzhou Pu Naier drying equipments Co., Ltd) mixing 10min；
(3) particle is made using efficient wet granulator in mixed raw material, included the following steps：By mixed feeding
Material is put into efficient wet granulator, and particle is made by the mesh of certain mesh number, the size of particle according to mesh size into
Row is adjusted；
(4) manufactured particle feed using the spray of fluid bed bottom is coated, coating solution is uniformly sprayed in feed table
Face, is made micro-encapsulated diet, and wherein coating solution uses the ethanol solution of zeins, using the spray of fluid bed bottom method into
Row coating, carries out bottom spray, process conditions are that inlet air temperature is 60 DEG C, are gone out with the ethanol solution of hot water soluble zeins
Air temperature is 30 DEG C, and bed temperature is 40 DEG C, atmospheric pressure 20MPa, and the flow velocity of peristaltic pump is 1mL/min, single crowd of yield 2kg, corn
The dosage of the ethanol solution of alcohol soluble protein is 100g；
(5) by the micro-encapsulated diet of preparation by 100 mesh, 80 mesh, 60 mesh, 40 mesh, 30 mesh, 20 mesh, 10 mesh shaking screen,
Grain size grading is carried out using sieve method；
(6) it is the feed being classified is for use in aluminium foil bag, being stored in 4 DEG C of refrigerator with vacuum packing machine sealing.
Comparative example 1
It is essentially identical with the preparation process of embodiment 1, it has only the difference is that preparing spotted maigre young postlarva micro-encapsulated diet
Lack medium chain triglyceride, decanoylacetaldehyde sodium hydrosulfite, 5-aminosalicylic acid in raw material.
Comparative example 2
It is essentially identical with the preparation process of embodiment 1, it has only the difference is that preparing spotted maigre young postlarva micro-encapsulated diet
Lack medium chain triglyceride in raw material.
Comparative example 3
It is essentially identical with the preparation process of embodiment 1, it has only the difference is that preparing spotted maigre young postlarva micro-encapsulated diet
Decanoylacetaldehyde sodium hydrosulfite in raw material.
Comparative example 4
It is essentially identical with the preparation process of embodiment 1, it has only the difference is that preparing spotted maigre young postlarva micro-encapsulated diet
Lack 5-aminosalicylic acid in raw material.
Comparative example 5
Using a kind of Chinese patent literature " spotted maigre young postlarva micro-encapsulated diet and preparation method thereof (Authorization Notice No.：
CN102845653B the technique of embodiment 1) " prepares spotted maigre young postlarva micro-encapsulated diet.
Culture experiment
Using the spotted maigre prelarva of 15 ages in days as test material, overall length 0.5cm.Spotted maigre prelarva is moved into 2
2.5m3Cement aquaculture pond in, put 20,000 tails per pond.Test group passes through the feed transition period of 5 days, i.e., is progressively dropped within the transition period
The dosage (reducing 20% daily) of low artemia, while it is stepped up the dosage (increasing by 20% daily) of micro-encapsulated diet.According to young
The size of postlarva and bore of ingesting, the grain size and dosage of micro-encapsulated diet increase with the growth of larva and juvenile.15-25 days ages
Between feed the micro-encapsulated diet (5.0-8.0mg/ tails/day) of 150-250 μm of grain size, be changed to 200-450 μm during 25-45 ages in days
Grain size.It is agreeable to the taste, appropriate, in due course to accomplish.Holding temperature of cultivation is 26 ± 2 DEG C during experiment, salinity is 30 ± 1 ‰, pH value 8.2
±0.2.In more than 6.0mg/L, ammonia-nitrogen content is less than 0.02mg/L for dissolved oxygen control, and content of nitrite is less than 0.01mg/L.Often
It inhales bottom of pond 1 time, to remove the residual bait of bottom of pond and dead seedling；The dirt of floating on water, quantity of exchanged water 80% are removed in time.Cultivate into
Row 30 days counts the survival rate of spotted maigre and the average length of spotted maigre.
As seen from the above table：(1) by the data of embodiment 1-3 and comparative example 5 as it can be seen that young using the spotted maigre young of embodiment 1
The average length and survival rate of fish micro-encapsulated diet have obtained comprehensive raising；Simultaneously by the data of embodiment 1-3 as it can be seen that embodiment
1 is optimum embodiment.
(2) by embodiment 1 and the data of comparative example 1-4 as it can be seen that medium chain triglyceride, decanoylacetaldehyde sodium hydrosulfite, 5-
Aminosalicylic acid plays synergistic effect during spotted maigre young postlarva micro-encapsulated diet is fed, and collaboration improves average length
And survival rate, this may be：
1) medium chain triglyceride is added in, the viscosity of fatty substance of high viscosity will greatly reduce, and can greatly improve food
The fuel factor of material and stomach, while can be mutual with decanoylacetaldehyde sodium hydrosulfite, 5-aminosalicylic acid to the assimilation effect of fat
With reference to being dispersed in feed and play stable effect, while in spotted maigre feeding process, can be dissociated the last of the ten Heavenly stems in stomach
The active ingredient of acyl acetaldehyde sodium hydrogensulfite, 5-aminosalicylic acid, so as to improve suction of the spotted maigre to feed and its active ingredient
It receives, so as to increase absorption efficiency, therefore adds the survival rate of spotted maigre and the average length of spotted maigre.
2) 5-aminosalicylic acid has significant antiinflammation to intestinal wall inflammation, due to having amino, hydroxyl and carboxyl on ring
Three active reactive groups, can be combined with each other with decanoylacetaldehyde sodium hydrosulfite, play the role of collaboration and promote anti-inflammatory,
It both can also promote metabolism in vivo simultaneously, while coordinate medium chain triglyceride as sustained release agent, the effect of sustained release be played, so as to increase
Add it in the spotted maigre in vivo profit reduction and reserving time, so as to add the average length of the survival rate of spotted maigre and spotted maigre.
3) decanoylacetaldehyde sodium hydrosulfite is sodium houttuyfonate, itself has certain antibiotic property and to the agreeable to the taste of fish
Property, while there is hydrophily and lipophile, it can be combined with medium chain triglyceride, easily be distributed to the effect that sustained release is played in feed
Fruit, while can interact with 5-aminosalicylic acid under acid instruction in stomach, it is promoted to be absorbed by the cell membrane of stomach
Enter in blood, so as to promote spotted maigre and vitality, therefore add the survival rate of spotted maigre and the average length of spotted maigre.
The present invention medium chain triglyceride, decanoylacetaldehyde sodium hydrosulfite, 5-aminosalicylic acid as reinforcement system, pass through
It is (0.5-0.1) to control medium chain triglyceride, decanoylacetaldehyde sodium hydrosulfite, the mass ratio of 5-aminosalicylic acid:(0.4-
0.8):(0.1-0.3) is realized in reinforcement system with medium chain triglyceride sustained release agent and stabilizer, 5-aminosalicylic acid conduct
Activating agent, while decanoylacetaldehyde sodium hydrosulfite is as sorbefacient so that reinforcement system applies to the spotted maigre of the present invention
The average length and survival rate of spotted maigre young postlarva can be effectively improved in larva and juvenile micro-encapsulated diet.
More than content is it cannot be assumed that present invention specific implementation is confined to these explanations, for the technical field of the invention
Those of ordinary skill for, under the premise of present inventive concept is not departed from, several simple deduction or replace can also be made, all should
When being considered as belonging to present invention scope of patent protection determined by the appended claims.
Claims (8)
1. a kind of spotted maigre young postlarva micro-encapsulated diet, which is characterized in that including the following raw material：White fish meal, krill meal, squid
Powder, fish protein hydrolysate levels, fodder yeast, alphalise starch, soybean lecithin, papain, fish oil, multi-vitamins, grandidierite
Substance, binding agent, immunopotentiator, phagostimulant, medium chain triglyceride, decanoylacetaldehyde sodium hydrosulfite, 5-aminosalicylic acid；Its
Described in binding agent be sodium alginate, the immunopotentiator be glucan and chitosan, the phagostimulant be taurine；It is described
Medium chain triglyceride, decanoylacetaldehyde sodium hydrosulfite, the mass ratio of 5-aminosalicylic acid are (0.5-0.1):(0.4-0.8):
(0.1-0.3)。
2. spotted maigre young postlarva micro-encapsulated diet according to claim 1, which is characterized in that the medium chain triglyceride,
Decanoylacetaldehyde sodium hydrosulfite, the mass ratio of 5-aminosalicylic acid are 0.8:0.6:0.2.
3. spotted maigre young postlarva micro-encapsulated diet according to claim 1, which is characterized in that in units of mass parts, bag
Include the following raw material：White fish meal 35-45 mass parts, krill meal 12-18 mass parts, squid powder 8-12 mass parts, fish protein hydrolysate levels
4-6 mass parts, fodder yeast 5-7 mass parts, alphalise starch 7.8-8 mass parts, soybean lecithin 4-6 mass parts, papain
1.5-2.5 mass parts, fish oil 2-4 mass parts, multi-vitamins 1.5-2.5 mass parts, composite mineral matter 1.5-2.5 mass parts,
Binding agent 0.8-1.2 mass parts, immunopotentiator 0.08-0.12 mass parts, phagostimulant 0.8-1.2 mass parts, medium chain triglyceride three
Ester 0.5-0.1 mass parts, decanoylacetaldehyde sodium hydrosulfite 0.4-0.8 mass parts, 5-aminosalicylic acid 0.1-0.3 mass parts.
4. spotted maigre young postlarva micro-encapsulated diet according to claim 3, which is characterized in that in units of mass parts, bag
Include the following raw material：40 mass parts of white fish meal, 15 mass parts of krill meal, 10 mass parts of squid powder, 5 mass parts of fish protein hydrolysate levels,
6 mass parts of fodder yeast, 7.9 mass parts of alphalise starch, 5 mass parts of soybean lecithin, 2 mass parts of papain, 3 mass of fish oil
Part, 2 mass parts of multi-vitamins, 2 mass parts of composite mineral matter, 1 mass parts of binding agent, 0.1 mass parts of immunopotentiator, food calling
1 mass parts of agent, 0.8 mass parts of medium chain triglyceride, 0.6 mass parts of decanoylacetaldehyde sodium hydrosulfite, 0.2 matter of 5-aminosalicylic acid
Measure part.
5. spotted maigre young postlarva micro-encapsulated diet according to claim 1, which is characterized in that in the profit immunopotentiator
Glucan is added with chitosan equal proportion.
A kind of 6. preparation method of spotted maigre young postlarva micro-encapsulated diet according to claim 1-5, which is characterized in that bag
Include following steps：
1) solid feed raw material is crushed with micronizer；
2) all feeds raw material in the ratio of feed formula is weighed, mixed, is first sufficiently mixed by hand, by 100 mesh screens,
Then mixed with mixer；
3) particle is made in mixed raw material；
4) manufactured particle feed using fluid bed bottom is sprayed or is coated with sugar coating machine, coating solution is uniformly sprayed and is being raised
Expect surface, micro-encapsulated diet is made, wherein coating solution is using the aqueous solution of gelatin, the ethanol solution of ethyl cellulose, corn alcohol
One kind in the ethanol solution of molten albumen, the ethanol solution of resin；
5) micro-encapsulated diet of preparation is subjected to grain size grading by the shaking screen of different meshes with sieve method；
6) feed being classified is sealed in vacuum packing machine in aluminium foil bag, be stored in 4 DEG C of refrigerator for use.
7. the preparation method of spotted maigre young postlarva micro-encapsulated diet according to claim 6, which is characterized in that in step 3)
Particle is made using one of following 3 kinds of methods：A. particle is made using fluid bed top spray method in mixed raw material, including such as
Lower step：Mixed material is placed in fluid bed, peristaltic pump spray into water, flow 0.5-1.5mL/min, fluid bed into
Air temperature is 60-80 DEG C, leaving air temp is 30-50 DEG C, 40-60 DEG C of bed temperature, compressed air pressure 20-30MPa, feed obtained
Product cut size is distributed between 150-600 μm；
B. particle is made using extrusion-spheronization in mixed raw material, included the following steps：Mixed material is added in into core
The water of material amount 5%-10% mixes 10- with trough type mixing machine with the process conditions of rotating speed 20-30rpm, 100-120 ° of batch turning angle
30min is made the uniform moist wood of moisture content, the moist wood prepared is placed in the stainless steel screen drum of rotating type granulating machine, through rotation
Turn to squeeze, cylindrical bars shape feed is formed by mesh, and be aided with the method for hand-crushed into wet granular, size controlling is in 150-
600 μm, wet granular is put into the rotating centrifugal disk of shot-blasting machine, pellet is made with rotating speed 5-15rpm throwing circles 20-40min；
C. particle is made using efficient wet granulator in mixed raw material, included the following steps：Mixed feed is put
Enter in efficient wet granulator, particle is made by the mesh of certain mesh number, the size of particle is adjusted according to the size of mesh
Section.
8. the preparation method of spotted maigre young postlarva micro-encapsulated diet according to claim 6, which is characterized in that in step 4)
It is coated using the method that fluid bed bottom is sprayed, carries out bottom spray with hot water soluble coating solution, process conditions are that inlet air temperature is
50-70 DEG C, leaving air temp is 20-40 DEG C, and bed temperature is 35-45 DEG C, atmospheric pressure 15-25MPa, and the flow velocity of peristaltic pump is 0.5-
1.5mL/min。
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110074254A (en) * | 2019-06-05 | 2019-08-02 | 甘肃省畜牧兽医研究所 | A method of saving fermentation apple pomace protein feed |
| CN110447773A (en) * | 2019-09-12 | 2019-11-15 | 广西鸿光农牧有限公司 | A kind of egg feedstuff and preparation method thereof improving laying rate |
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| CN102845653A (en) * | 2012-10-15 | 2013-01-02 | 浙江省海洋水产研究所 | Nibea albiflora richardson larvae and juvenile microcapsule feed, as well as processing preparation and feeding method thereof |
| CN105639120A (en) * | 2015-12-29 | 2016-06-08 | 湖南晶天科技实业有限公司 | Cyclodextrin inclusion compound containing antibiotic components, a compounding method thereof and feed additive |
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2017
- 2017-12-31 CN CN201711494007.3A patent/CN108077651A/en not_active Withdrawn
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102845653A (en) * | 2012-10-15 | 2013-01-02 | 浙江省海洋水产研究所 | Nibea albiflora richardson larvae and juvenile microcapsule feed, as well as processing preparation and feeding method thereof |
| CN105639120A (en) * | 2015-12-29 | 2016-06-08 | 湖南晶天科技实业有限公司 | Cyclodextrin inclusion compound containing antibiotic components, a compounding method thereof and feed additive |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110074254A (en) * | 2019-06-05 | 2019-08-02 | 甘肃省畜牧兽医研究所 | A method of saving fermentation apple pomace protein feed |
| CN110447773A (en) * | 2019-09-12 | 2019-11-15 | 广西鸿光农牧有限公司 | A kind of egg feedstuff and preparation method thereof improving laying rate |
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