CN107988388A - A kind of and relevant SNP marker of pig dewclaw undue growth character and application thereof - Google Patents
A kind of and relevant SNP marker of pig dewclaw undue growth character and application thereof Download PDFInfo
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- CN107988388A CN107988388A CN201711310397.4A CN201711310397A CN107988388A CN 107988388 A CN107988388 A CN 107988388A CN 201711310397 A CN201711310397 A CN 201711310397A CN 107988388 A CN107988388 A CN 107988388A
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Abstract
The present invention provides a kind of and relevant SNP marker of pig dewclaw undue growth character, the site of the SNP marker is the 133470903rd nucleotide site on international pig reference gene group No. 13 chromosomes of 11.1 version, and the base in the site is T or G.The present invention can reduce the generation of great Bai dewclaw undue growths, accelerate genetic progress, effectively improve the economic benefit of kind of pig breeding by the advantage allele of the preferably SNP.
Description
Technical field
The present invention relates to a kind of and relevant SNP marker of pig dewclaw undue growth character and application thereof.
Background technology
Pig is typical Artiodactyla mammal, its 1st toe is degenerated completely;3rd, 4 toes are especially flourishing, and length is equal, claim
Be main hoof;2nd, 5 toes are undeveloped, hang stand in the air under normal circumstances, be referred to as dewclaw (see Fig. 1).The main function of dewclaw
The weight that is to aid in main hoof to bear pig and its balance is maintained, (be more than 7cm) when sow dewclaw is long, easily occur
Mechanical damage, in turn resulting in can not stand and diet, secondary to cause farrowing sow miscarriage, milking sow malnutrition etc. (see figure
2), so as to be had an impact to each side of sow, sow is directly eliminated when serious, and huge economy is brought to breeding enterprise
Loss.
Due to pig dewclaw final lengths after adult just it is observed that, and the seed selection of boar is before this, therefore early
The phase accuracy of selection is just particularly important.And separate and identify that pig dewclaw undue growth susceptibility loci is then that pig dewclaw is excessive
Grow the key of breeding for disease resistance.At present, whole-genome association (Genome-wide association studies,
GWAS) it is widely used in finding the site with livestock and poultry complex character highlights correlations, the molecular labeling filtered out can be to being difficult to
The character (such as dewclaw undue growth) of measurement carries out molecular marker assisted selection (the marker assisted of early stage
Selection, MAS) breeding.At present, this method has become the important means of large scale of pig farm industry molecular breeding.
Dewclaw undue growth is distributed widely in bacon hogs kind, is the determinant for causing the numerous sow of energy to be eliminated in advance
One of.Great Bai is one of bacon hogs kind famous in the world, because of its higher reproductive capacity, preferable growing and fattening and adaptation
The features such as property, often by as the synthetic female parent of many market pigs.Multiple studies have shown that there is obvious hang in large white sow colony
Hoof undue growth phenomenon.Therefore, genetic improvement is carried out to the dewclaw growth traits of large white sow, will it is possible to prevente effectively from sow because
Limb hoof is abnormal and is eliminated in advance, improves the service life of sow to greatest extent, is carried so as to reduce sow because of toe hoof illness
Before be eliminated the economic loss of brought breeding enterprise.
The content of the invention
It is an object of the present invention to provide a kind of and the relevant SNP marker of pig dewclaw undue growth character and its use
On the way.
To achieve the above object, the technical solution used in the present invention:It is a kind of relevant with pig dewclaw undue growth character
SNP marker, the site of the SNP marker is the 133470903rd on international pig reference gene group No. 13 chromosomes of 11.1 version
Nucleotide site, the base in the site is T or G.
Preferably, the sequence of the SNP marker such as SEQ ID NO:Shown in 1, the SEQ ID NO:Sequence shown in 1 is from 5 '
It is T or G to have held the 332nd bit base.The SNP site of the molecular labeling corresponds to 11.1 version of international pig reference gene group 13
T at the 133470903rd on chromosome>G is mutated;The SNP site of the molecular labeling is SEQ ID NO1:It is shown, wherein sequence
M in row is T or G, causes the difference of dewclaw growth traits.The present invention can be subtracted by the advantage allele of the preferably SNP
The generation of few great Bai dewclaw undue growths, accelerates genetic progress, effectively improves the economic benefit of kind of pig breeding.
The present invention provides a kind of primer pair for being used to detect SNP marker described above, the nucleic acid sequence of the primer pair
Row such as SEQ ID NO:2 and SEQ ID NO:Shown in 3.
The present invention provides a kind of kit for being used to detect SNP marker described above, including primer described above
It is right.
The present invention provides a kind of method for reducing pig dewclaw undue growth, the described method comprises the following steps:
Detect the 133470903rd nucleotide site on No. 13 chromosomes of international 11.1 version of pig reference gene group of pig
Genotype, selects the GG types individual of the 133470903rd nucleotide site to be used as boar.
Preferably, the 133470903rd on No. 13 chromosomes of international 11.1 version of pig reference gene group of the detection pig
The method of the genotype of nucleotide site comprises the following steps:
(1) genomic DNA of pig to be measured is extracted;
(2) using the primer pair described in claim 3, the genomic DNA of the pig to be measured is subjected to PCR amplification, so as to
Obtain pcr amplification product;
(3) pcr amplification product is sequenced, to obtain sequencing result;
(4) sequencing result is based on, determines the gene of the SNP marker as claimed in claim 1 or 2 of the pig to be measured
Type.
Preferably, the boar includes its synthesis system of Herba Phaii tankervilliaes.
The present invention provides the purposes of SNP marker described above in pig dewclaw undue growth is reduced.
The present invention provides the use of primer pair described above or the kit in pig dewclaw undue growth is reduced
On the way.
The present invention provides SNP marker described above or the primer pair or the kit in identification pig
Purposes in dewclaw undue growth character.
The beneficial effects of the present invention are:
The present invention studies and determines to influence the relevant molecular labeling of large white sow dewclaw undue growth, there is provided one kind is used to reflect
The fixing primer pair for ringing large white sow dewclaw undue growth trait molecular marker, finally establishes the molecular labeling auxiliary of efficiently and accurately
Breeding technique, is applied in boar dewclaw growth traits genetic improvement, so as to reduce pig dewclaw undue growth, reduces sow
Generation mechanical damage, it is secondary to cause farrowing sow miscarriage, milking sow malnutrition etc., so as to each of sow and offspring piglet
Aspect performance has an impact;Sow service life is improved, improves enterprise profit, increases core competitiveness.
Brief description of the drawings
Fig. 1 is pig dewclaw schematic diagram;
Fig. 2 is dewclaw is normal and dewclaw undue growth schematic diagram;
Fig. 3 is that full-length genome of the large white sow on dewclaw growth traits on No. 13 chromosomes associates (GWAS) analysis
Figure;Wherein:Abscissa represents the chromosome numbers of pig;Ordinate expression-logP is worth.
Embodiment
With reference to embodiment and attached drawing, the present invention is described in further detail, but embodiments of the present invention are unlimited
In this.
What the foregoing invention purpose of the present invention was specifically realized in:
1st, swinery is tested
Experiment swinery colony used in the present invention is boar branch company of Guangdong Wen'S Foodstuffs Group Co., Ltd.
475 purebred large white sows, are boar branch company core group, and colony's pedigree record is detailed.Using identical feeding manner by special
Industry personnel are raised and are managed.Swinery is raised according to unified pig trophic level, free choice feeding and drinking-water.Experiment pig is good for
Health, well-grown, no clinical symptoms.
2nd, sample collection and phenotype record
Above-mentioned pig ear tissue sample is collected, is soaked in 75% ethanol that to be placed in -20 DEG C of refrigerators spare.
Record pig dewclaw growth traits:Two back leg dewclaws measure of all pigs is using moccasin ruler according to unification side
Method carries out precisive, and accuracy is millimeter.The direction eyed to the front with pig is denoted as left and right directions, and the dewclaw of hoof is surveyed behind left and right
The length of fixed outside dewclaw, each dewclaw measure measure 3 times, take its average value to be recorded as the outstanding of the rear hoof dewclaw of pig or so respectively
Hoof length.
3rd, the extracting of tissue DNA and pig full-length genome 80K SNP sentence type
The ear tissue of above-mentioned large white sow is gathered, ear tissue is soaked in 75% ethanol to be placed in -20 DEG C of refrigerators standby in time
With.With reference to the complete genome DNA of phenol chloroform method extraction large white sow, with Nanodrop-ND1000 nucleic acid concentrations instrument and agar
Sugared gel electrophoresis carries out concentration mensuration and quality testing to the DNA of large white sow.Specifically nucleic acid concentration instrument is measured
For A260/280 ratios in 1.8-2.0, A260/230 ratios are determined as purity qualification in 1.7-1.9, by concentration higher than 300 nanograms/
Microlitre it is determined as concentration qualification;By the DNA sample of purity and concentration qualification be uniformly diluted to 50 nanograms/microlitre.6 μ l diluted again
DNA sample mixed with 2 μ lLoading Buffer, be loaded in 1% Ago-Gel, electrophoresis 25min under 150V voltages,
Observe and take pictures under UV detector and gel imaging equipment, observe the integrity degree of DNA.By concentration, purity and complete
The all qualified DNA sample of degree is determined as up-to-standard sample.
GeneSeek Genomic Profiler Porcine 80K SNP parting platforms, using Illumina
The operation instruction and normal process of Infinium carries out chip hybridization and is scanned with result.Read finally by GenomeStudio softwares
Take genotype data.Quality control is carried out to the genotype data of acquisition with PLINK v1.07, specific standards are as follows:
(1) Hardy-Weinberg equilibrium (HWE, Hardy-Weinberg equilibrium) examines P values to be more than 10-6
(2) SNP recall rates are more than 90%
(3) individual SNP recall rates are more than 90%
(4) SNP minimums gene frequency (MAF, Minor Allele Frequency) is more than 5%
(5) SNP on unknown position and sex chromosome is deleted
4th, full-length genome association (GWAS) analysis
Using case-control (Case-Control) whole-genome association based on SNP marker, by detecting full base
Because case is individual on each SNP marker site in the range of group and compares the difference of individual allelic and genotype frequency,
The site that linkage disequilibrium is in the gene locus of control targe character is searched with this.This research selection comes from Michigan, United States
The GEMMA softwares that the Xiang Zhou of university and the Matthew Stephens of Chicago University are researched and developed jointly hang large white sow
Hoof undue growth colony and normal dewclaw colony carry out whole-genome association.GEMMA softwares are one and are carrying out full genome
The brute force software of colony's layering and affiliation can be explained in group association analysis, is adapted to use single variable linear mixed model
(Univariate Linear Mixed Model) is associated detection to single phenotype and mark, explains that colony is layered with this
With composition of sample effect, and for estimate genotype explain phenotype ratio.In view of affiliation and colony's layering effect
The possibility of false positive results, this research are controlled by using single argument mixed linear model caused by tackling association analysis, are led to
Cross GEMMA softwares and carry out whole-genome association.Single argument mixed linear model:
Y=W α+x β+u+ ε
Y is phenotype vector;For age in days when W is represented using field border effect and sampling as the oriental matrix of covariant, α is covariant
Corresponding related coefficient;The oriental matrix that x is SNP (for affiliation matrix after correction, is calculated by full-length genome marker genetype
And obtain), β is the effect of SNP;μ is the stochastic effects of prediction, and ε is residual error.Determine that full-length genome associates using Bonferroni methods
Conspicuousness threshold value is analyzed to obtain, the remarkable threshold of genomic level is 0.05 divided by effective SNP site quantity, i.e. 0.05/50227=
9.9548E-07;Chromosome level remarkable threshold is 1 divided by effective SNP site quantity, i.e. 1/50227=1.9910E-05.
GWAS analysis results are as shown in Figure 3.As can be seen from Figure 3, it is outstanding in the presence of significantly affecting in No. 13 chromosome in large white sow colony
The site of hoof growth traits, g.332T most strongly connected SNP is>G (P=1.04E-06).
5th, the correlation analysis of different genotype and dewclaw growth phenotype
According to table 1, the SNP site of molecular labeling is g.332T>The G and extremely significantly correlated (P=of dewclaw growth traits
1.04E-06), illustrate that this molecular labeling significantly affects the dewclaw growth traits of pig, the auxiliary of this SNP site to pig can be passed through
Selection is helped, so as to reduce colony's dewclaw undue growth.In addition, normal growth group in SNP g.332T>The allele in G sites
The gene frequency pole of T is substantially less than undue growth group (5.4%<20.5%, P<0.0001).Therefore, we are being educated
, it is necessary to eliminate the boar of TT and TG types during kind, retain the boar of GG types, to improve the allele G in the site by generation
Frequency.
The different gene frequencies of table 1 and corresponding phenotypic difference
6th, target DNA sequence amplification and sequencing
(1) it is 13 to contain with the amplification purpose fragment of the purpose fragment of the significantly correlated SNP site of great Bai dewclaw undue growths
The nucleotide sequence of one section of 483bp in number chromosome, the upstream and downstream primer of sequence amplification are:
Sense primer 5 '-AGAAGAATCTATGGGAAACTAC-3 ' (SEQ ID NO:2);
Anti-sense primer 5 '-GTAAGTGGGAGCGGGTAG-3 ' (SEQ ID NO:3).
(2) system and condition setting of PCR amplification
Configure 20ul systems, wherein DNA sample 3.5ul, sense primer 0.6ul, anti-sense primer 0.6ul, PCR mix
10ul,ddH2O 5.3ul, PCR condition are 98 DEG C of pre-degeneration 2min, and 98 DEG C of denaturation 30s, 50.8 DEG C of annealing 30s, 72 DEG C extend
30s, totally 35 circulations, finally extend to 72 DEG C of 10min.
(3) DNA sequence dna sequencing identification:Sequence is carried out in Shenzhen Huada Genetic Technology Co., Ltd, and genetic fragment is surveyed
Positive and negative two reactions.By measured sequence and NCBI genomic sequence comparisons, the mutation of corresponding SNP site is drawn.
Sequencing result (SEQ ID NO as follows:1):
Note:The M marked in sequence table is mutational site, (is mutating alkali yl in bracket, is equipotential with display is underlined
Gene mutation), it is shown as primer sequence in the head and the tail overstriking of the sequence.
It should be noted that the above embodiments are merely illustrative of the technical solutions of the present invention rather than model protected to the present invention
The limitation enclosed, although being explained in detail with reference to preferred embodiment to the present invention, it will be understood by those of ordinary skill in the art that,
Can be to technical scheme technical scheme is modified or replaced equivalently, without departing from the essence and model of technical solution of the present invention
Enclose.
Sequence table
<110>Agricultural University Of South China;Guangdong Wen'S Foodstuffs Group Co., Ltd.
<120>A kind of and relevant SNP marker of pig dewclaw undue growth character and application thereof
<160> 3
<170> PatentIn version 3.3
<210> 1
<211> 483
<212> DNA
<213>Pig
<220>
<221> misc_feature
<223>M=T or G
<400> 1
agaagaatct atgggaaact accaaaaccc cccacaaaac accaaggaga ggaaaaaaca 60
caagttgcgt ttgatttaaa aaatatttat aggacacggt ttttattttc atagaatgtc 120
aataaatatt ttaccaggat gatacggata tatcacaatg atgggaggaa gaatttggga 180
tcgctaaggg atggaaacat ttcgaccttt aatttaaacc tcgattcagc aggcttatcc 240
ttggcagtgg gggggtcttg aaatctggca gagaaagcgt ccttacgtgc acagaccaga 300
tttctaacgt gggcacgctg ccagatcagt amatggagga ccagtggcac cggctcttga 360
tgacctgaag caaaggcttc ctgaaagtga ggaagatgac agtgcaggcc accagcaggg 420
tgaggcagct gggaagaatg agcacgaggt acagcaggcc catgtctacc cgctcccact 480
tac 483
<210> 2
<211> 22
<212> DNA
<213>Artificial sequence
<400> 2
agaagaatct atgggaaact ac 22
<210> 3
<211> 18
<212> DNA
<213>Artificial sequence
<400> 3
gtaagtggga gcgggtag 18
Claims (10)
1. a kind of and relevant SNP marker of pig dewclaw undue growth character, it is characterised in that the site of the SNP marker is state
133470903rd nucleotide site on pig reference gene group No. 13 chromosomes of 11.1 version of border, the base in the site is T or G.
2. SNP marker according to claim 1, it is characterised in that the sequence of the SNP marker such as SEQ ID NO:1 institute
Show, the SEQ ID NO:The 332nd bit base from 5 ' ends of sequence shown in 1 is T or G.
3. a kind of primer pair for being used to detect SNP marker as claimed in claim 1 or 2, it is characterised in that the primer pair
Nucleotide sequence such as SEQ ID NO:2 and SEQ ID NO:Shown in 3.
4. a kind of kit for being used to detect SNP marker as claimed in claim 1 or 2, it is characterised in that will including such as right
Seek the primer pair described in 3.
A kind of 5. method for reducing pig dewclaw undue growth, it is characterised in that the described method comprises the following steps:
Detect the gene of the 133470903rd nucleotide site on No. 13 chromosomes of international 11.1 version of pig reference gene group of pig
Type, selects the GG types individual of the 133470903rd nucleotide site to be used as boar.
6. according to the method described in claim 5, it is characterized in that, international 11.1 version of pig reference gene group of the detection pig
The method of the genotype of the 133470903rd nucleotide site comprises the following steps on No. 13 chromosomes:
(1) genomic DNA of pig to be measured is extracted;
(2) using the primer pair described in claim 3, the genomic DNA of the pig to be measured is subjected to PCR amplification, to obtain
Pcr amplification product;
(3) pcr amplification product is sequenced, to obtain sequencing result;
(4) sequencing result is based on, determines the genotype of the SNP marker as claimed in claim 1 or 2 of the pig to be measured.
7. according to the method described in claim 5, it is characterized in that, the boar includes its synthesis system of Herba Phaii tankervilliaes.
8. purposes of the SNP marker as claimed in claim 1 or 2 in pig dewclaw undue growth is reduced.
9. primer pair as claimed in claim 3 or kit as claimed in claim 4 are in pig dewclaw undue growth is reduced
Purposes.
10. SNP marker as claimed in claim 1 or 2 or primer pair as claimed in claim 3 or such as claim 4 institute
Purposes of the kit stated in pig dewclaw undue growth character is identified.
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Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN108359735A (en) * | 2018-05-19 | 2018-08-03 | 华中农业大学 | With the SNP genetic markers of the relevant VTCN1 genes of sow limb coffin bone density |
CN108359736A (en) * | 2018-05-23 | 2018-08-03 | 华中农业大学 | The SNP genetic markers of sow limb coffin bone density character |
CN113637768A (en) * | 2021-08-06 | 2021-11-12 | 华南农业大学 | SNP molecular marker on pig No. 13 chromosome related to number of malformed piglets born by sow and application thereof |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20040241676A1 (en) * | 2001-04-24 | 2004-12-02 | Leif Andersson | Methods |
CN103421769A (en) * | 2012-10-23 | 2013-12-04 | 华中农业大学 | SNP molecular marker related to pig carcass trait and application thereof |
-
2017
- 2017-12-11 CN CN201711310397.4A patent/CN107988388B/en active Active
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20040241676A1 (en) * | 2001-04-24 | 2004-12-02 | Leif Andersson | Methods |
CN103421769A (en) * | 2012-10-23 | 2013-12-04 | 华中农业大学 | SNP molecular marker related to pig carcass trait and application thereof |
Non-Patent Citations (4)
Title |
---|
WU_ABGC: "dbSNP登记号:rs81210648", 《NCBI》 * |
温鹏: "蹄部异常对泌乳母猪行为的影响", 《今日养猪业》 * |
郭广伦: "猪趾蹄病发病原因及防治措施", 《农村百事通》 * |
陈慧勇 等: "猪的全基因组水平SNP研究现状", 《生命科学》 * |
Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN108359735A (en) * | 2018-05-19 | 2018-08-03 | 华中农业大学 | With the SNP genetic markers of the relevant VTCN1 genes of sow limb coffin bone density |
CN108359735B (en) * | 2018-05-19 | 2020-12-25 | 华中农业大学 | SNP genetic marker of VTCN1 gene related to sow limb hoof bone density |
CN108359736A (en) * | 2018-05-23 | 2018-08-03 | 华中农业大学 | The SNP genetic markers of sow limb coffin bone density character |
CN108359736B (en) * | 2018-05-23 | 2020-12-29 | 华中农业大学 | SNP genetic marker for sow limb hoof bone density character |
CN113637768A (en) * | 2021-08-06 | 2021-11-12 | 华南农业大学 | SNP molecular marker on pig No. 13 chromosome related to number of malformed piglets born by sow and application thereof |
CN113637768B (en) * | 2021-08-06 | 2023-07-14 | 华南农业大学 | SNP molecular marker related to number of sow-produced malformed piglets on chromosome 13 of pig and application thereof |
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