CN107981056A - The preparation method of feed for sheep is prepared using compound bacteria and disease-resistant Chinese herbal medicine - Google Patents
The preparation method of feed for sheep is prepared using compound bacteria and disease-resistant Chinese herbal medicine Download PDFInfo
- Publication number
- CN107981056A CN107981056A CN201711243820.3A CN201711243820A CN107981056A CN 107981056 A CN107981056 A CN 107981056A CN 201711243820 A CN201711243820 A CN 201711243820A CN 107981056 A CN107981056 A CN 107981056A
- Authority
- CN
- China
- Prior art keywords
- parts
- disease
- chinese herbal
- herbal medicine
- sheep
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/10—Feeding-stuffs specially adapted for particular animals for ruminants
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/30—Oligoelements
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Animal Husbandry (AREA)
- Zoology (AREA)
- Food Science & Technology (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Health & Medical Sciences (AREA)
- Physiology (AREA)
- Botany (AREA)
- Mycology (AREA)
- Inorganic Chemistry (AREA)
- Sustainable Development (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Birds (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention discloses the preparation method that feed for sheep is prepared using compound bacteria and disease-resistant Chinese herbal medicine, belong to technique for producing feed field, the preparation method includes the preparation of compound bacteria, the preparation of disease-resistant Chinese herbal medicine extract, feedstuff processing, material mixed fermentation, drying and other steps；Trametes versicolor and microbacterium in the composite bacteria that the present invention uses, which have, destroys cell membrane, the degraded effect such as crude fibre and pectin, and with saccharomycete with the use of synergistic effect can be produced, cell membrane, the degraded crude fibre in sugarcane caudal lobe and stalk can be destroyed while fermentation so that the supplement material prepared is easier to be digested and assimilated by sheep；In addition, the disease-resistant Chinese herbal medicine extract added in feed for sheep can improve the percentage of neutrophil in blood, you can to effectively improve the non-specific immunity of sheep, reduce sick rate.
Description
Technical field
The present invention relates to technique for producing feed field, is raised more particularly to sheep is prepared using compound bacteria and disease-resistant Chinese herbal medicine
The preparation method of material.
Background technology
Chinese herbal medicine comes from natural plants, and toxic side effect is small, the katabolism easily in animal body, good drug efficacy, livestock products medicine
Thing residual quantity is low and some Chinese herbal medicines have the function that to promote growth of animal etc., in order to reduce the sick probability of livestock and poultry, improves body
Immunity, generally improves immunity of organisms using antibiotic is added in feed, and improvement is grown, but antibiotic etc
Long-time service can make microorganism produce drug resistance, cause livestock and poultry endogenous infection and two degree of infection, decline immunity of livestock, antibiosis
The problems such as element residual, seriously affect animal health and health.At present, more and more antibiotic are prohibited from using；Therefore,
Quote Chinese herbal medicine and raised to develop the non-stimulated high disease-resistant feed of new type of safe for alleviating medicament residue and pathogenic bacteria resistance to drugs etc.
Material and food-safety problem, ensure that human health has positive meaning.
But current application of the Chinese herbal medicine in feed is added substantial amounts of in traditional Chinese herbal feed there is also some problems
Chinese herbal medicine, so as to have impact on the palatability of feed, Chinese patent 201510143866.2 discloses a kind of raising in applying disease-resistant
Ability Chinese herbal medicine sheep medicine feed and preparation method thereof, which is made of the raw material of following parts by weight：40-120 parts of soya-bean cake,
30-60 parts of wheat bran, 30-60 parts of rice bran, 10-30 parts of pears slag, 10-20 parts of treaster, 2-6 parts of salt；Chinese herb feed additive 1-10
Part, it is prepared by the preparation and fermenting raw materials of Chinese herb feed additive.Using 18 kinds of Chinese herbal medicines mix merga pass it is common plus
The mode that fry is boiled obtains Chinese herb feed additive, although preparation process is simple, the addition of a large amount of Chinese herbal medicines can produce hardship
Astringent taste, influences the palatability of feed, and Chinese herbal medicine effective ingredients utilization rate is low, therefore, it is necessary to the preparation process of development of new, subtracts
Bitter taste that is low or even removing Chinese herbal medicine, improves the palatability of feed, while the active principle of Chinese herbal medicine is greatly incorporated
Into feed, the utilization rate of Chinese herbal medicine effective ingredients is improved.
The disclosure of background above technology contents is only used for inventive concept and the technical solution that auxiliary understands the present invention, it is not
The prior art of present patent application is necessarily belonged to, shows the applying date of the above in present patent application in no tangible proof
In the case of disclosed, above-mentioned background technology should not be taken to the novelty and creativeness of evaluation the application.
The content of the invention
Present invention aims at proposing a kind of preparation method that feed for sheep is prepared using compound bacteria and disease-resistant Chinese herbal medicine, with
Solve the technical problems such as above-mentioned middle feed palatability is poor, disease resistance is low；The feed for sheep of the present invention can be realized effectively at the same time
Sheep getting fat.
To solve the above problems, the present invention provides following technical scheme：
The preparation method that feed for sheep is prepared using compound bacteria and disease-resistant Chinese herbal medicine is comprised the following steps：
S1：The preparation of compound bacteria：
S11：By Sucus Caulis et Folium Brassicae capitatae 0.5g/l-1g/l, peptone 2.5g/l-5g/l, glucose 0.7g/l-1.2g/l, chlorination
Sodium 0.4g/l-0.9g/l, potassium phosphate 0.8g/l-1.3g/l are uniformly mixed, and sterilizing, obtains culture medium；
S12：Trametes versicolor after purification, microbacterium, saccharomycete are linked into the culture medium of step T1, are cultivated per 100g
In base, inoculum concentration 0.5-1.0ml；
S13：The culture medium quiescent culture 6-9d of Trametes versicolor, microbacterium, saccharomycete will be accessed under 28-30 DEG C of environment,
Period shakes up once manually every 2d；Compound bacteria is made；
S2：The preparation of disease-resistant Chinese herbal medicine extract：
S21：All raw materials of disease-resistant Chinese herbal medicine extract are cleaned, mixing, which is put into, to decoct in container and add clear water, clearly
Water exceeds raw material surface 2-3cm, is decocted 1 time at a temperature of 70-90 DEG C, and filtering, filter residue is again with the water of 2-3 times of parts by weight in 50-
Decoct 1-2 times, filter, merging filtrate at a temperature of 80 DEG C；
The disease-resistant Chinese herbal medicine extract is in units of parts by weight, including following raw material：5-7 parts of wrinkled giant hyssop, cordate houttuynia 6-8
Part, 2-4 parts of coptis root, 8-10 parts of garlic, 2-4 parts of purple perilla, 8-10 parts of STEVIA REBAUDIANA, 6-8 parts of rhizoma zingiberis, 2-4 parts of Radix Codonopsis, radix glycyrrhizae 8-10
Part, 7-9 parts of jujube；
S22：It is the mixed of 1.20-1.30 that the filtrate that step S21 merges is concentrated into relative density under cryogenic high pressure environment
Close liquid A；
S23：Additional proportion is 0.5-0.7 in the mixed liquor A of step S22:The water of 1.0-1.2 and the mixed liquid B of ethanol,
20-30min is stirred under Ultrasonic Conditions；Obtain mixed liquor C；
S24：Mixed liquor C is centrifuged under the conditions of 30-40 DEG C of temperature, rotating speed 1200-2400r/min, is taken
Clear liquid；Supernatant is spray-dried, obtains disease-resistant Chinese herbal medicine extract；
S3：Feedstuff processing：Sugarcane caudal lobe and peanut straw are pulverized and mixed, mixed-powder and wheat bran, the beans of gained
The dregs of rice, pears slag, treaster are uniformly mixed, and mixture D is made；
S4：Material mixed fermentation：Mixture D and disease-resistant Chinese herbal medicine are extracted under the conditions of 28-30 DEG C, 600-800r/min
Thing, amino acid, trace element, compound bacteria are uniformly mixed, then uniformly mixed material is fermented 5-7d with diaphragm seal；
S5：It is dry：It is 30%-40% to air-dry or dry in the shade the material fermented to water content, that is, obtains feed for sheep.
Further, the step S12 Trametes versicolors, microbacterium, the ratio of saccharomycete are 1:1:0.5.
Further, the step S22 cryogenic high pressures condition is 10-15 DEG C of temperature, pressure 1.02-1.5MPa.
Further, the disease-resistant Chinese herbal medicine extracts of step S21 are in units of parts by weight, including following raw material：Wrinkled giant hyssop 6
Part, 7 parts of cordate houttuynia, 3 parts of coptis root, 9 parts of garlic, 3 parts of purple perilla, 9 parts of STEVIA REBAUDIANA, 7 parts of rhizoma zingiberis, 3 parts of Radix Codonopsis, 9 parts of radix glycyrrhizae, jujube 8
Part.
Further, the step S23 ultrasonic technique conditions are：40-55 DEG C of temperature, power 35-45W, frequency 20-
25KHz。
Further, the ratio of mixed liquor A and mixed liquid B described in the step S23 is 1-2:0.8.
Further, the mixed-powder mesh number after the step S3 is crushed is 25-45 mesh.
Further, the step S4 amino acid is one kind in tryptophan, lysine, threonine；The trace element
For any two kinds in iron, zinc, selenium, manganese, chromium.
Further, the fermentation condition of the step S5 is：25-28 DEG C of temperature, humidity 60%-70%.
Further, the process conditions of the step S5 spray drying are：Speed 1000-1500ml/h, feeding temperature 65-
70 DEG C, 30-40 DEG C of drop temperature, atomizing pressure 1.5-2.15MPa.
The beneficial effect that the present invention is compared with the prior art includes：
1st, the Trametes versicolor in the composite bacteria that uses of the present invention and microbacterium have destroy cell membrane, degraded crude fibre and
Pectin etc. acts on, and with saccharomycete with the use of can produce synergistic effect, can be destroyed while fermenting sugarcane caudal lobe and
Cell membrane, degraded crude fibre in stalk so that the supplement material prepared is easier to be digested and assimilated by sheep.
2nd, wrinkled giant hyssop in the disease-resistant herbal raw material that adds of the present invention, cordate houttuynia, coptis root, garlic, purple perilla, STEVIA REBAUDIANA, rhizoma zingiberis,
Radix Codonopsis, radix glycyrrhizae, jujube play synergistic effect, and wrinkled giant hyssop is used cooperatively with coptis root, garlic, have change it is turbid and in, purging intense heat and detonicating, sterilization
The effect of stomach invigorating, Radix Codonopsis, jujube are with the use of having a tonifying middle-Jiao and Qi, the effect of strengthen immunity, and cordate houttuynia is with antibacterial, disease-resistant
Poison, improve immunity of organisms and other effects, and rhizoma zingiberis can drive exterior cold, pre- avian influenza-prevention with purple perilla use；And STEVIA REBAUDIANA primarily serves
Seasoning acts on, and avoids the Chinese herbal medicine that boils excessively bitter, radix glycyrrhizae primarily serves the effect of coordinating the drug actions of a prescription so that ten taste Chinese herbal medicines are more
Add rational compatibility, it is respective the effect of reach maximization.
3rd, it is thin in vain can to effectively improve neutrophilia in blood for the disease-resistant Chinese herbal medicine extract added in feed for sheep of the present invention
The percentage of born of the same parents, you can to effectively improve the non-specific immunity of sheep, reduce sick rate.
Embodiment
For ease of more fully understanding the present invention, compound bacteria and disease-resistant Chinese herbal medicine are applied to the preparation of feed for sheep by the present invention
During, and be illustrated by following embodiments, these embodiments belong to protection scope of the present invention, but do not limit the present invention
Protection domain.
Embodiment 1：
Feed for sheep is in units of parts by weight, including following raw material：70 parts of sugarcane caudal lobe, 60 parts of peanut straw, wheat bran 70
Part, 40 parts of dregs of beans, 10 parts of compound bacteria, 10 parts of treaster, 5 parts of pears slag, 12 parts of tryptophan, ferro element and 2 parts of Zn-ef ficiency, it is disease-resistant in
8 parts of herb extracts；
The compound bacteria obtains by the following method：
T1：Culture medium：By Sucus Caulis et Folium Brassicae capitatae 0.5g/l, peptone 2.5g/l, glucose 0.7g/l, sodium chloride 0.4g/l, phosphorus
Sour potassium 0.8g/l is uniformly mixed, and sterilizing, obtains culture medium；
T2：Inoculation：Trametes versicolor after purification, microbacterium, saccharomycete are linked into the culture medium of step T1, each strain
Ratio be 1:1:0.5, often in 100g culture mediums, inoculum concentration 0.5ml；
T3：Prepare compound bacteria：The culture medium standing training of Trametes versicolor, microbacterium, saccharomycete will be accessed under 28 DEG C of environment
6d is supported, is during which shaken up manually once every 2d；Compound bacteria is made.
The disease-resistant Chinese herbal medicine extract is in units of parts by weight, including following raw material：5 parts of wrinkled giant hyssop, 6 parts of cordate houttuynia, Huang
2 parts of lotus, 8 parts of garlic, 2 parts of purple perilla, 8 parts of STEVIA REBAUDIANA, 6 parts of rhizoma zingiberis, 2 parts of Radix Codonopsis, 8 parts of radix glycyrrhizae, 7 parts of jujube；
The disease-resistant Chinese herbal medicine extract obtains by the following method：
D1：All raw materials of disease-resistant Chinese herbal medicine extract are cleaned, mixing, which is put into, decocts in container and add clear water, clear water
Beyond raw material surface 2cm, decoct 1 time, filter at a temperature of 70 DEG C, filter residue is decocted with the water of 2 times of parts by weight at a temperature of 50 DEG C again
Boil 1 time, filter, merging filtrate,
D2：By the filtrate that step D1 merges 10 DEG C of temperature, pressure 1.02MPa cryogenic high pressure environment under be concentrated into it is opposite
Density is 1.20 mixed liquor A；
D3：In the mixed liquor A of step D2 additional proportion be 0.51.0 water and ethanol mixed liquid B, the mixed liquor A
Ratio with mixed liquid B is 1:0.8；40 DEG C of temperature, power 35W, frequency 20KHz Ultrasonic Conditions under stir 20min；
To mixed liquor C；
D4：Mixed liquor C is centrifuged under the conditions of temperature is 30 DEG C, rotating speed is 1200r/min, takes supernatant；
D5：Under the conditions of speed 1000ml/h, 65 DEG C of feeding temperature, 30 DEG C of drop temperature, atomizing pressure 1.5MPa, it will walk
The supernatant of rapid D4 is spray-dried, and obtains disease-resistant Chinese herbal medicine extract.
The present invention also provides the preparation method of above-mentioned feed for sheep；The preparation method comprises the following steps：
S1：Pretreatment of raw material：Sugarcane caudal lobe and peanut straw are pulverized and mixed, the mixed-powder mesh number after crushing is 25-
45 mesh；The mixed-powder of gained is uniformly mixed with wheat bran, dregs of beans, pears slag, treaster, mixture D is made；
S2：Fermenting raw materials：Under the conditions of 28 DEG C, 600r/min by mixture D and disease-resistant Chinese herbal medicine extract, tryptophan,
Ferro element and Zn-ef ficiency, compound bacteria are uniformly mixed, then by uniformly mixed material diaphragm seal in 25 DEG C of temperature, humidity 60%
Under the conditions of ferment 5d；
S3：It is dry：It is 30% to air-dry or dry in the shade the material fermented to water content, that is, obtains feed for sheep.
Embodiment 2：
Feed for sheep is in units of parts by weight, including following raw material：80 parts of sugarcane caudal lobe, 65 parts of peanut straw, wheat bran 80
Part, 50 parts of dregs of beans, 15 parts of compound bacteria, 12 parts of treaster, 8 parts of pears slag, 13 parts of threonine, Zn-ef ficiency and 4 parts of selenium element, it is disease-resistant in
10 parts of herb extracts；
The compound bacteria obtains by the following method：
T1：Culture medium：By Sucus Caulis et Folium Brassicae capitatae 0.8g/l, peptone 3.8g/l, glucose 1.0g/l, sodium chloride 0.6g/l, phosphorus
Sour potassium 1.0g/l is uniformly mixed, and sterilizing, obtains culture medium；
T2：Inoculation：Trametes versicolor after purification, microbacterium, saccharomycete are linked into the culture medium of step T1, each strain
Ratio be 1:1:0.5, often in 100g culture mediums, inoculum concentration 0.8ml；
T3：Prepare compound bacteria：The culture medium standing training of Trametes versicolor, microbacterium, saccharomycete will be accessed under 29 DEG C of environment
7d is supported, is during which shaken up manually once every 2d；Compound bacteria is made.
The disease-resistant Chinese herbal medicine extract is in units of parts by weight, including following raw material：6 parts of wrinkled giant hyssop, 7 parts of cordate houttuynia, Huang
3 parts of lotus, 9 parts of garlic, 3 parts of purple perilla, 9 parts of STEVIA REBAUDIANA, 7 parts of rhizoma zingiberis, 3 parts of Radix Codonopsis, 9 parts of radix glycyrrhizae, 8 parts of jujube；
The disease-resistant Chinese herbal medicine extract obtains by the following method：
D1：All raw materials of disease-resistant Chinese herbal medicine extract are cleaned, mixing, which is put into, decocts in container and add clear water, clear water
Beyond raw material surface 3cm, decoct 1 time, filter at a temperature of 80 DEG C, filter residue decocts 2 with the water of 3 times of parts by weight in 65 DEG C of temperature again
It is secondary, filtering, merging filtrate,
D2：By the filtrate that step D1 merges 17 DEG C of temperature, pressure 1.26MPa cryogenic high pressure environment under be concentrated into it is opposite
Density is 1.25 mixed liquor A；
D3：Additional proportion is 0.6 in the mixed liquor A of step D2:1.1 water and the mixed liquid B of ethanol, the mixed liquor
The ratio of A and mixed liquid B is 1.6:0.8；Temperature 50 C, power 40W, frequency 23KHz Ultrasonic Conditions under stir
125min；Obtain mixed liquor C；
D4：Mixed liquor C is centrifuged under the conditions of temperature is 30-40 DEG C, rotating speed is 1200-2400r/min, is taken
Supernatant；
D5：, will under the conditions of speed 1200ml/h, 68 DEG C of feeding temperature, 35 DEG C of drop temperature, atomizing pressure 1.83MPa
The supernatant of step D4 is spray-dried, and obtains disease-resistant Chinese herbal medicine extract.
The present invention also provides the preparation method of above-mentioned feed for sheep；The preparation method comprises the following steps：
S1：Pretreatment of raw material：Sugarcane caudal lobe and peanut straw are pulverized and mixed, the mixed-powder mesh number after crushing is 35
Mesh；The mixed-powder of gained is uniformly mixed with wheat bran, dregs of beans, pears slag, treaster, mixture D is made；
S2：Fermenting raw materials：Under the conditions of 29 DEG C, 700r/min by mixture D and disease-resistant Chinese herbal medicine extract, threonine,
Zn-ef ficiency and selenium element, compound bacteria are uniformly mixed, then by uniformly mixed material diaphragm seal in 28 DEG C of temperature, humidity 65%
Under the conditions of ferment 6d；
S3：It is dry：It is 35% to air-dry or dry in the shade the material fermented to water content, that is, obtains feed for sheep.
Embodiment 3：
Feed for sheep is in units of parts by weight, including following raw material：90 parts of sugarcane caudal lobe, 70 parts of peanut straw, wheat bran 90
It is part, 60 parts of dregs of beans, 20 parts of compound bacteria, 14 parts of treaster, 10 parts of pears slag, 14 parts of lysine, ferro element and 6 parts of manganese element, disease-resistant
12 parts of Chinese herbal medicine extract；
The compound bacteria obtains by the following method：
T1：Culture medium：By Sucus Caulis et Folium Brassicae capitatae 1g/l, peptone 5g/l, glucose 1.2g/l, sodium chloride 0.9g/l, potassium phosphate
1.3g/l is uniformly mixed, and sterilizing, obtains culture medium；
T2：Inoculation：Trametes versicolor after purification, microbacterium, saccharomycete are linked into the culture medium of step T1, each strain
Ratio be 1:1:0.5, often in 100g culture mediums, inoculum concentration 1.0ml；
T3：Prepare compound bacteria：The culture medium standing of Trametes versicolor, microbacterium, saccharomycete will be accessed under 28-30 DEG C of environment
9d is cultivated, is during which shaken up manually once every 2d；Compound bacteria is made.
The disease-resistant Chinese herbal medicine extract is in units of parts by weight, including following raw material：7 parts of wrinkled giant hyssop, 8 parts of cordate houttuynia, Huang
4 parts of lotus, 10 parts of garlic, 4 parts of purple perilla, 10 parts of STEVIA REBAUDIANA, 8 parts of rhizoma zingiberis, 4 parts of Radix Codonopsis, 10 parts of radix glycyrrhizae, 9 parts of jujube；
The disease-resistant Chinese herbal medicine extract obtains by the following method：
D1：All raw materials of disease-resistant Chinese herbal medicine extract are cleaned, mixing, which is put into, decocts in container and add clear water, clear water
Beyond raw material surface 3cm, decoct 1 time, filter at a temperature of 90 DEG C, filter residue is decocted with the water of 3 times of parts by weight at a temperature of 80 DEG C again
Boil 2 times, filter, merging filtrate,
D2：By the filtrate that step D1 merges 15 DEG C of temperature, pressure 1.5MPa cryogenic high pressure environment under be concentrated into it is opposite
Density is 1.30 mixed liquor A；
D3：Additional proportion is 0.7 in the mixed liquor A of step D2:1.2 water and the mixed liquid B of ethanol, the mixed liquor
The ratio of A and mixed liquid B is 2:0.8；55 DEG C of temperature, power 45W, frequency 25KHz Ultrasonic Conditions under stir 30min；
Obtain mixed liquor C；
D4：Mixed liquor C is centrifuged under the conditions of temperature is 40 DEG C, rotating speed is 2400r/min, takes supernatant；
D5：, will under the conditions of speed 1500ml/h, 70 DEG C of feeding temperature, 40 DEG C of drop temperature, atomizing pressure 2.15MPa
The supernatant of step D4 is spray-dried, and obtains disease-resistant Chinese herbal medicine extract.
The present invention also provides the preparation method of above-mentioned feed for sheep；The preparation method comprises the following steps：
S1：Pretreatment of raw material：Sugarcane caudal lobe and peanut straw are pulverized and mixed, the mixed-powder mesh number after crushing is 45
Mesh；The mixed-powder of gained is uniformly mixed with wheat bran, dregs of beans, pears slag, treaster, mixture D is made；
S2：Fermenting raw materials：Under the conditions of 30 DEG C, 800r/min by mixture D and disease-resistant Chinese herbal medicine extract, amino acid,
Trace element, compound bacteria are uniformly mixed, then by uniformly mixed material diaphragm seal under the conditions of 28 DEG C of temperature, humidity 70%
Ferment 7d；
S3：It is dry：It is 30%-40% to air-dry or dry in the shade the material fermented to water content, up to feed for sheep.
Comparative example 1：
Feed for sheep is in units of parts by weight, including following raw material：80 parts of sugarcane caudal lobe, 65 parts of peanut straw, wheat bran 80
Part, 50 parts of dregs of beans, 15 parts of saccharomycete, 12 parts of treaster, 8 parts of pears slag, 13 parts of threonine, Zn-ef ficiency and 4 parts of selenium element, it is disease-resistant in
10 parts of herb extracts；
The disease-resistant Chinese herbal medicine extract is in units of parts by weight, including following raw material：6 parts of wrinkled giant hyssop, 7 parts of cordate houttuynia, Huang
3 parts of lotus, 9 parts of garlic, 3 parts of purple perilla, 9 parts of STEVIA REBAUDIANA, 7 parts of rhizoma zingiberis, 3 parts of Radix Codonopsis, 9 parts of radix glycyrrhizae, 8 parts of jujube；
The disease-resistant Chinese herbal medicine extract obtains by the following method：
D1：All raw materials of disease-resistant Chinese herbal medicine extract are cleaned, mixing, which is put into, decocts in container and add clear water, clear water
Beyond raw material surface 3cm, decoct 1 time, filter at a temperature of 80 DEG C, filter residue is decocted with the water of 3 times of parts by weight at a temperature of 65 DEG C again
Boil 2 times, filter, merging filtrate,
D2：By the filtrate that step D1 merges 17 DEG C of temperature, pressure 1.26MPa cryogenic high pressure environment under be concentrated into it is opposite
Density is 1.25 mixed liquor A；
D3：It is 0.6 that Volume fraction is added in the mixed liquor A of step D2:1.1 water and the mixed liquid B of ethanol, it is described
The Volume fraction of mixed liquor A and mixed liquid B is 1.6:0.8；Temperature 50 C, power 40W, frequency 23KHz ultrasonic wave bar
125min is stirred under part；Obtain mixed liquor C；
D4：Mixed liquor C is centrifuged under the conditions of temperature is 30-40 DEG C, rotating speed is 1200-2400r/min, is taken
Supernatant；
D5：, will under the conditions of speed 1200ml/h, 68 DEG C of feeding temperature, 35 DEG C of drop temperature, atomizing pressure 1.83MPa
The supernatant of step D4 is spray-dried, and obtains disease-resistant Chinese herbal medicine extract.
The preparation method of above-mentioned feed for sheep comprises the following steps：
S1：Pretreatment of raw material：Sugarcane caudal lobe and peanut straw are pulverized and mixed, the mixed-powder mesh number after crushing is 35
Mesh；The mixed-powder of gained is uniformly mixed with wheat bran, dregs of beans, pears slag, treaster, mixture D is made；
S2：Fermenting raw materials：Under the conditions of 29 DEG C, 700r/min by mixture D and disease-resistant Chinese herbal medicine extract, amino acid,
Trace element, saccharomycete are uniformly mixed, then by uniformly mixed material diaphragm seal under the conditions of 28 DEG C of temperature, humidity 65%
Ferment 6d；
S3：It is dry：It is 35% to air-dry or dry in the shade the material fermented to water content, up to feed for sheep.
Comparative example 2
The technical solution of comparative example 2 and the technical solution of embodiment 2 are essentially identical, difference lies in：
The disease-resistant Chinese herbal medicine extract obtains by the following method：
D1：All raw materials of disease-resistant Chinese herbal medicine extract are cleaned, mixing, which is put into, decocts in container and add clear water, clear water
Beyond raw material surface 3cm, decoct 1 time, filter at a temperature of 80 DEG C, filter residue is decocted with the water of 3 times of parts by weight at a temperature of 65 DEG C again
Boil 2 times, filter, merging filtrate；The ethanol solution of the filtrate volume fraction 0.5% is added in the filtrate of merging, mixing is equal
It is even, obtain mixed liquor A；
D2：, will under the conditions of speed 1200ml/h, 68 DEG C of feeding temperature, 35 DEG C of drop temperature, atomizing pressure 1.83MPa
The mixed liquor A of step D1 is spray-dried, and obtains disease-resistant Chinese herbal medicine extract.
Other preparation processes are identical with the preparation process of embodiment 2.
Comparative example 3：
The technical solution of comparative example 3 and the technical solution of embodiment 2 are essentially identical, difference lies in：
The disease-resistant Chinese herbal medicine extract is in units of parts by weight, including following raw material：9 parts of STEVIA REBAUDIANA, 7 parts of cordate houttuynia,
3 parts of purple perilla, 7 parts of rhizoma zingiberis；
Other preparation processes are identical with the preparation process of embodiment 2.
Comparative example 4：
The technical solution of comparative example 4 and the technical solution of embodiment 2 are essentially identical, difference lies in：
The disease-resistant Chinese herbal medicine extract is in units of parts by weight, including following raw material：6 parts of wrinkled giant hyssop, 3 parts of coptis root, garlic 9
Part, 3 parts of Radix Codonopsis, 9 parts of radix glycyrrhizae, 8 parts of jujube；
Other preparation processes are identical with the preparation process of embodiment 2.
Comparative example 5：
Using patent " one kind improves disease resistance Chinese herbal medicine sheep medicine feed and preparation method thereof " (application number：
201510143866.2) technical solution of the embodiment 1-5 in prepares sheep medicine feed.
Effect example：
Feed for sheep is prepared using the technical solution of embodiment 1-3 and comparative example 1-4 respectively, while using in comparative example 5
The technical solution of embodiment 1-5 prepares sheep medicine feed.Selection 80 is 14 ± 1kg sheep (30 age in days) without illness record and weight；
8 groups are divided into, every group 10, the feed prepared using above-mentioned technical proposal carries out nursing experiment, and daily feeding twice, and is
Morning, evening feeding, experimental period are 40 days, and every 10 day morning weighs once on an empty stomach, in the jugular vein of sheep on the day of off-test
Place takes a blood sample once, blood volume 5ml.The palatability such as institute of table 1 of the rate of body weight gain of sheep, sick rate result and supplement material after the test
Show；Leucocyte investigation result is as shown in table 2 in sheep blood.
Table 1：
Table 2：
It was found from the embodiment 2 of table 1 and comparative example 1-2, if individually using saccharomycetes to make fermentation raw material or Chinese herbal juice not
Concentrated directly extraction, centrifuging and taking supernatant, then the feed palatability that is produced is poor, sheep weightening unobvious and sheep easy infection
Germ causes sick；Because the Trametes versicolor and microbacterium in the composite bacteria that the present invention uses, which have, destroys cell membrane, degraded
Crude fibre and pectin etc. act on, and can be destroyed while fermentation sweet with saccharomycete with the use of that can produce synergistic effect
Cell membrane, degraded crude fibre in sugarcane caudal lobe and stalk so that the supplement material prepared is easier to be digested and assimilated by sheep.This
Outside, the sheep prepared using technical solution of the present invention is can be seen that from embodiment 2 and comparative example 1-4 and is supplemented and is expected, palatability is preferable,
And it is full of nutrition, sheep weightening is obvious after eating, and without sick phenomenon, illustrates Chinese herbal medicine treatment process using the present invention, that is, lead to
Cross concentrated medicine juice and active principle is concentrated into collection, then supernatant, each close phase of processing step are extracted through extraction, centrifugal treating
Even, preferably the active ingredient concentration extraction in Chinese herbal medicine can be come out, greatly improves the utilization rate of active principle；At the same time
Pass through compound bacteria-fermented so that the active ingredient and amino acid of Chinese herbal medicine extract, trace element spread or penetrate into well
In supplement material, the nutritive value of supplement material is improved.
Neutrophil percentage in sheep blood is related to the nospecific immunity of sheep；Within the specific limits, in thermophilic
Property leucocyte percentage is higher, then the non-specific immunity of sheep is stronger.It was found from the embodiment 1-3 of table 2, embodiment 1-3
Between neutrophil percentage difference unobvious, but compared with comparative example 1-5, more than 3 percentage points will be higher by, said
Bright addition Chinese herbal medicine extract can effectively improve the percentage of neutrophil in blood, you can to effectively improve the non-of sheep
Specific immunity ability, reduces sick rate.In addition, from embodiment 2 and comparative example 3-4 understand wrinkled giant hyssop, cordate houttuynia, coptis root, garlic,
Purple perilla, STEVIA REBAUDIANA, rhizoma zingiberis, Radix Codonopsis, radix glycyrrhizae, jujube play synergistic effect, and wrinkled giant hyssop is used cooperatively with coptis root, garlic, turbid with changing
With in, purging intense heat and detonicating, sterilization stomach invigorating the effect of, Radix Codonopsis, jujube are with the use of having a tonifying middle-Jiao and Qi, the effect of strengthen immunity,
Cordate houttuynia has antibacterial, antiviral, raising immunity of organisms and other effects, and rhizoma zingiberis can drive exterior cold, prevention fowl stream with purple perilla use
Sense；And STEVIA REBAUDIANA primarily serves seasoning effect, avoid the Chinese herbal medicine that boils excessively bitter, radix glycyrrhizae primarily serves the work of coordinating the drug actions of a prescription
With so that ten more rational compatibilities of taste Chinese herbal medicine, it is respective the effect of reach maximization.
The palatability of the sheep feed of the present invention is preferable, and sheep rate of body weight gain is obviously improved, and the neutrophil percentage of sheep compares
It is high.
Above content is to combine preferred embodiment further description made for the present invention, it is impossible to assert this hair
Bright specific implementation is confined to these explanations.For general technical staff of the technical field of the invention, do not taking off
On the premise of present inventive concept, it can also make some replacements or modification to the embodiment that these have been described, and these are replaced
Generation or variant should all be considered as belonging to protection scope of the present invention.
Claims (10)
1. the preparation method of feed for sheep is prepared using compound bacteria and disease-resistant Chinese herbal medicine, it is characterised in that the preparation method bag
Include following steps：
S1：The preparation of compound bacteria：
S11：By Sucus Caulis et Folium Brassicae capitatae 0.5g/l-1g/l, peptone 2.5g/l-5g/l, glucose 0.7g/l-1.2g/l, sodium chloride
0.4g/l-0.9g/l, potassium phosphate 0.8g/l-1.3g/l are uniformly mixed, and sterilizing, obtains culture medium；
S12：Trametes versicolor after purification, microbacterium, saccharomycete are linked into the culture medium of step T1, per 100g culture mediums
In, inoculum concentration 0.5-1.0ml；
S13：The culture medium quiescent culture 6-9d of Trametes versicolor, microbacterium, saccharomycete will be accessed under 28-30 DEG C of environment, during which
Shaken up manually once every 2d；Compound bacteria is made；
S2：The preparation of disease-resistant Chinese herbal medicine extract：
S21：All raw materials of disease-resistant Chinese herbal medicine extract are cleaned, mixing, which is put into, to decoct in container and add clear water, and clear water surpasses
Go out raw material surface 2-3cm, decocted 1 time at a temperature of 70-90 DEG C, filtered, filter residue is again with the water of 2-3 times of parts by weight at 50-80 DEG C
At a temperature of decoct 1-2 times, filter, merging filtrate；
The disease-resistant Chinese herbal medicine extract is in units of parts by weight, including following raw material：5-7 parts of wrinkled giant hyssop, 6-8 parts of cordate houttuynia, Huang
2-4 parts of lotus, 8-10 parts of garlic, 2-4 parts of purple perilla, 8-10 parts of STEVIA REBAUDIANA, 6-8 parts of rhizoma zingiberis, 2-4 parts of Radix Codonopsis, 8-10 parts of radix glycyrrhizae, jujube
7-9 parts；
S22：The filtrate that step S21 merges is concentrated into the mixed liquor that relative density is 1.20-1.30 under cryogenic high pressure environment
A；
S23：Additional proportion is 0.5-0.7 in the mixed liquor A of step S22:The water of 1.0-1.2 and the mixed liquid B of ethanol, super
20-30min is stirred under the conditions of sound wave；Obtain mixed liquor C；
S24：Mixed liquor C is centrifuged under the conditions of 30-40 DEG C of temperature, rotating speed 1200-2400r/min, takes supernatant；
Supernatant is spray-dried, obtains disease-resistant Chinese herbal medicine extract；
S3：Feedstuff processing：Sugarcane caudal lobe and peanut straw are pulverized and mixed, mixed-powder and wheat bran, dregs of beans, the pears of gained
Slag, treaster are uniformly mixed, and mixture D is made；
S4：Material mixed fermentation：Under the conditions of 28-30 DEG C, 600-800r/min by mixture D and disease-resistant Chinese herbal medicine extract,
Amino acid, trace element, compound bacteria are uniformly mixed, then uniformly mixed material is fermented 5-7d with diaphragm seal；
S5：It is dry：It is 30%-40% to air-dry or dry in the shade the material fermented to water content, that is, obtains feed for sheep.
2. the preparation method of feed for sheep is prepared using compound bacteria and disease-resistant Chinese herbal medicine as claimed in claim 1, it is characterised in that
The step S12 Trametes versicolors, microbacterium, the ratio of saccharomycete are 1:1:0.5.
3. the preparation method of feed for sheep is prepared using compound bacteria and disease-resistant Chinese herbal medicine as claimed in claim 1, it is characterised in that
The step S22 cryogenic high pressures condition is 10-15 DEG C of temperature, pressure 1.02-1.5MPa.
4. the preparation method of feed for sheep is prepared using compound bacteria and disease-resistant Chinese herbal medicine as claimed in claim 1, it is characterised in that
The disease-resistant Chinese herbal medicine extracts of step S21 are in units of parts by weight, including following raw material：6 parts of wrinkled giant hyssop, 7 parts of cordate houttuynia, coptis root
3 parts, 9 parts of garlic, 3 parts of purple perilla, 9 parts of STEVIA REBAUDIANA, 7 parts of rhizoma zingiberis, 3 parts of Radix Codonopsis, 9 parts of radix glycyrrhizae, 8 parts of jujube.
5. the preparation method of feed for sheep is prepared using compound bacteria and disease-resistant Chinese herbal medicine as claimed in claim 1, it is characterised in that
The step S23 ultrasonic technique conditions are：40-55 DEG C of temperature, power 35-45W, frequency 20-25KHz.
6. the preparation method of feed for sheep is prepared using compound bacteria and disease-resistant Chinese herbal medicine as claimed in claim 1, it is characterised in that
The ratio of mixed liquor A and mixed liquid B described in the step S23 is 1-2:0.8.
7. the preparation method of feed for sheep is prepared using compound bacteria and disease-resistant Chinese herbal medicine as claimed in claim 1, it is characterised in that
Mixed-powder mesh number after the step S3 is crushed is 25-45 mesh.
8. the preparation method of feed for sheep is prepared using compound bacteria and disease-resistant Chinese herbal medicine as claimed in claim 1, it is characterised in that
The step S4 amino acid is tryptophan, one kind in lysine, threonine；The trace element is iron, in zinc, selenium, manganese, chromium
Any two kinds.
9. the preparation method of feed for sheep is prepared using compound bacteria and disease-resistant Chinese herbal medicine as claimed in claim 1, it is characterised in that
The fermentation condition of the step S5 is：25-28 DEG C of temperature, humidity 60%-70%.
10. preparing the preparation method of feed for sheep using compound bacteria and disease-resistant Chinese herbal medicine as claimed in claim 1, its feature exists
In the process conditions of the step S5 spray drying are：Speed 1000-1500ml/h, 65-70 DEG C of feeding temperature, drop temperature
30-40 DEG C, atomizing pressure 1.5-2.15MPa.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711243820.3A CN107981056A (en) | 2017-11-30 | 2017-11-30 | The preparation method of feed for sheep is prepared using compound bacteria and disease-resistant Chinese herbal medicine |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711243820.3A CN107981056A (en) | 2017-11-30 | 2017-11-30 | The preparation method of feed for sheep is prepared using compound bacteria and disease-resistant Chinese herbal medicine |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107981056A true CN107981056A (en) | 2018-05-04 |
Family
ID=62034827
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711243820.3A Pending CN107981056A (en) | 2017-11-30 | 2017-11-30 | The preparation method of feed for sheep is prepared using compound bacteria and disease-resistant Chinese herbal medicine |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107981056A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109527232A (en) * | 2018-12-30 | 2019-03-29 | 广西武鸣绿世界生态农业投资有限公司 | High nutrition straw biological Black Hills sheep feed and preparation method thereof |
| CN114041536A (en) * | 2021-11-24 | 2022-02-15 | 石河子大学 | Composite Chinese herbal medicine microecological preparation and preparation method thereof |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104222628A (en) * | 2014-08-26 | 2014-12-24 | 陈文文 | Composite Chinese herb medicine feed additive |
| CN104286568A (en) * | 2014-10-27 | 2015-01-21 | 安徽省田丰牧业科技有限公司 | Chinese herbal medicine feed for ewes at suckling period and preparation method of Chinese herbal medicine feed |
| CN104585477A (en) * | 2014-12-11 | 2015-05-06 | 黄南概 | Chinese herbal medicine mixed fermentation feed and preparation method thereof |
| CN104770592A (en) * | 2015-03-30 | 2015-07-15 | 杨文昊 | Disease resistance improving Chinese herbal medicine sheep feed and preparation method thereof |
| CN105265802A (en) * | 2015-11-03 | 2016-01-27 | 界首市田营镇美地养殖家庭农场 | Formula feed special for young white goats |
| CN105325710A (en) * | 2015-11-06 | 2016-02-17 | 陈琳 | Goat feed and preparation method thereof |
| CN105995057A (en) * | 2016-05-24 | 2016-10-12 | 松桃天农养殖场 | Sheep fodder and preparation method thereof |
-
2017
- 2017-11-30 CN CN201711243820.3A patent/CN107981056A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104222628A (en) * | 2014-08-26 | 2014-12-24 | 陈文文 | Composite Chinese herb medicine feed additive |
| CN104286568A (en) * | 2014-10-27 | 2015-01-21 | 安徽省田丰牧业科技有限公司 | Chinese herbal medicine feed for ewes at suckling period and preparation method of Chinese herbal medicine feed |
| CN104585477A (en) * | 2014-12-11 | 2015-05-06 | 黄南概 | Chinese herbal medicine mixed fermentation feed and preparation method thereof |
| CN104770592A (en) * | 2015-03-30 | 2015-07-15 | 杨文昊 | Disease resistance improving Chinese herbal medicine sheep feed and preparation method thereof |
| CN105265802A (en) * | 2015-11-03 | 2016-01-27 | 界首市田营镇美地养殖家庭农场 | Formula feed special for young white goats |
| CN105325710A (en) * | 2015-11-06 | 2016-02-17 | 陈琳 | Goat feed and preparation method thereof |
| CN105995057A (en) * | 2016-05-24 | 2016-10-12 | 松桃天农养殖场 | Sheep fodder and preparation method thereof |
Non-Patent Citations (2)
| Title |
|---|
| 任南琪等: "《污染控制微生物学》", 30 September 2004, 哈尔滨工业大学出版社 * |
| 李莉等: "《秸秆生物降解技术研究与应用》", 28 February 2014, 辽宁科学技术出版社 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109527232A (en) * | 2018-12-30 | 2019-03-29 | 广西武鸣绿世界生态农业投资有限公司 | High nutrition straw biological Black Hills sheep feed and preparation method thereof |
| CN114041536A (en) * | 2021-11-24 | 2022-02-15 | 石河子大学 | Composite Chinese herbal medicine microecological preparation and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101130741B (en) | Vinegar koji and biological edible vinegar, and method of producing the same | |
| CN103146560B (en) | Health-care vinegar and preparation method thereof | |
| CN105482953B (en) | A kind of production technology of selenium-rich blueberry mixed health wine | |
| CN103168979B (en) | Miracle fruit high-fiber jelly health-care food for regulating blood sugar level | |
| CN107574073B (en) | Brewing method of stomach-invigorating health-preserving yellow wine | |
| CN106434157A (en) | Traditional Chinese medicinal distiller's yeast and preparation method | |
| CN106047631A (en) | Strawberry vinegar prepared through enzymolysis and application thereof | |
| CN101407754A (en) | Production process of black fungus rough rice vinegar | |
| CN102021101B (en) | Preparation method of healthy wine for preventing and treating cardiovascular and cerebrovascular diseases | |
| CN107080246A (en) | A kind of plant enzyme and preparation method thereof | |
| CN107981056A (en) | The preparation method of feed for sheep is prepared using compound bacteria and disease-resistant Chinese herbal medicine | |
| CN108902853A (en) | A kind of method of honey concentration | |
| CN107927366A (en) | New disease-resistant lamb supplement material of height and preparation method thereof | |
| CN107212392A (en) | A kind of full nutrition fermentation health-preserving food and preparation method thereof | |
| CN1374396A (en) | Making process of nutritious lycium chinense and date vinegar | |
| KR20100020862A (en) | Method of preparing fermented liquid of garlic and chonggugjang | |
| CN107981055A (en) | The adult feed for sheep prepared using crop material | |
| CN110192571A (en) | A kind of preparation method of sweet potato red bean Semen Coicis modulation cream | |
| CN105505707B (en) | A kind of health drink based on Oyster yellow wine and preparation method thereof | |
| KR101935848B1 (en) | Food composition for improving hyperglycemia using Brassica rapa | |
| CN108013238A (en) | Disease-resistant Chinese herbal medicine extract of lamb materials and preparation method thereof | |
| CN108013237A (en) | High-effective service and its application in lamb supplement material is prepared | |
| KR101453061B1 (en) | Ferment solution and pill using mulberry and manufacturing method of that | |
| CN107410809A (en) | A kind of mushroom with abundant selenium solid beverage of cancer-resisting | |
| CN1076962A (en) | The product of edible mushrooms and herbal medicine mixed fermentation brewing and method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20180504 |
|
| RJ01 | Rejection of invention patent application after publication |