CN107907520A - One kind separation preserves reagent filtering detection device - Google Patents
One kind separation preserves reagent filtering detection device Download PDFInfo
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- CN107907520A CN107907520A CN201711318648.3A CN201711318648A CN107907520A CN 107907520 A CN107907520 A CN 107907520A CN 201711318648 A CN201711318648 A CN 201711318648A CN 107907520 A CN107907520 A CN 107907520A
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- 238000001914 filtration Methods 0.000 title claims abstract description 79
- 239000003153 chemical reaction reagent Substances 0.000 title claims abstract description 61
- 238000001514 detection method Methods 0.000 title claims abstract description 32
- 238000000926 separation method Methods 0.000 title claims abstract description 21
- 238000007789 sealing Methods 0.000 claims abstract description 96
- 238000006243 chemical reaction Methods 0.000 claims abstract description 57
- 239000007788 liquid Substances 0.000 claims abstract description 31
- 238000002955 isolation Methods 0.000 claims abstract description 25
- 239000000565 sealant Substances 0.000 claims abstract description 19
- 239000012528 membrane Substances 0.000 claims abstract description 13
- 210000003205 muscle Anatomy 0.000 claims description 18
- 230000006835 compression Effects 0.000 claims description 10
- 238000007906 compression Methods 0.000 claims description 10
- 241000521257 Hydrops Species 0.000 claims description 7
- 206010030113 Oedema Diseases 0.000 claims description 7
- 238000002156 mixing Methods 0.000 claims description 5
- 239000006004 Quartz sand Substances 0.000 claims description 3
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 3
- 229910052782 aluminium Inorganic materials 0.000 claims description 3
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 claims description 3
- 239000005030 aluminium foil Substances 0.000 claims description 3
- 238000004891 communication Methods 0.000 claims description 3
- 230000005484 gravity Effects 0.000 claims description 3
- 229910052751 metal Inorganic materials 0.000 claims description 3
- 239000002184 metal Substances 0.000 claims description 3
- 230000002906 microbiologic effect Effects 0.000 claims description 3
- 239000007787 solid Substances 0.000 claims description 3
- 210000001519 tissue Anatomy 0.000 claims description 3
- 239000000463 material Substances 0.000 claims description 2
- 230000000903 blocking effect Effects 0.000 claims 1
- 238000007689 inspection Methods 0.000 claims 1
- 244000005700 microbiome Species 0.000 abstract description 19
- 238000000034 method Methods 0.000 abstract description 5
- 238000011002 quantification Methods 0.000 abstract description 4
- 230000008569 process Effects 0.000 abstract description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 12
- 230000006872 improvement Effects 0.000 description 6
- 230000000813 microbial effect Effects 0.000 description 6
- 238000012360 testing method Methods 0.000 description 6
- 239000007850 fluorescent dye Substances 0.000 description 5
- 244000000010 microbial pathogen Species 0.000 description 5
- 239000003795 chemical substances by application Substances 0.000 description 4
- 238000011109 contamination Methods 0.000 description 4
- 238000013461 design Methods 0.000 description 3
- 238000000605 extraction Methods 0.000 description 3
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 238000006073 displacement reaction Methods 0.000 description 2
- 239000000446 fuel Substances 0.000 description 2
- 230000001788 irregular Effects 0.000 description 2
- 230000014759 maintenance of location Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 208000031295 Animal disease Diseases 0.000 description 1
- 241000235342 Saccharomycetes Species 0.000 description 1
- 238000009360 aquaculture Methods 0.000 description 1
- 244000144974 aquaculture Species 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 235000013361 beverage Nutrition 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 230000001010 compromised effect Effects 0.000 description 1
- 238000012937 correction Methods 0.000 description 1
- 239000002537 cosmetic Substances 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000008676 import Effects 0.000 description 1
- 238000011169 microbiological contamination Methods 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 238000001303 quality assessment method Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 239000013535 sea water Substances 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
- G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
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- Health & Medical Sciences (AREA)
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- Chemical & Material Sciences (AREA)
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- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Optics & Photonics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
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- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
One kind separation preserves reagent filtering detection device, including seal cap, filter pump head and filtering reaction tube, seal cap is compressible, include first kind reagent, filtering reaction tube is located in filtering pump head, filtering reaction bottom of the tube is equipped with sealing-plug, the filter membrane that can retain microorganism is equipped with filtering reaction tube, filtering reaction tube upper end is connected by support ring with seal cap, support ring bottom is equipped with the sealing isolation layer for being used for placing the second class reagent, hollow sealing bar is located in support ring and seal cap, sealing bar upper end is equipped with the head cover of sealant, the sharp protrusion for puncturing sealant is convexly equipped with head cover, it is raised that seal cap top inner wall is equipped with up-side down triangle, sealing bar bottom is equipped with the protrusion for being used for puncturing separation layer.The present invention is rational in infrastructure ingenious, easy to operate, is incubated and operates without full shading, just directly can carry out accurate quantification to the thing content to be checked in liquid, simplify detection process, save detection time and cost, has the characteristics that to detect sensitive, fast, accurately.
Description
Technical field
The invention belongs to technical field of microbial detection, one kind separation preserves agent detection devices, more particularly, to one kind
The microorganism of organic and water environment microorganism is extracted and device for fast detecting.
Background technology
Content of microorganisms test has become what municipal water use, food and beverage, clinic, cosmetics and pharmaceutical field were faced
One challenge.But China is in research of the fields such as food, medicine, pesticide, soil, environment to microbial contamination and related mark
Accurate and method is more, and the microbial contamination in fuel and fuel system still belongs to new field in China, in oil product industry category
Newer things, the also all external imports of used detection device, method are with high costs.
On the other hand, although China's quality standard of water environment (GB3838-2002) defines 24 elementary item standard limits
It is worth (23 physical and chemical indexes and 1 pathogenic microorganism index), but current China's water quality assessment is very examined less still based on physical and chemical index
Consider pathogenic microorganism index.Study (Yang Yong etc., 2013) to show, the major water system in China has been subject to different degrees of cause of disease
It is also pathogenic microorganism institute that microbial contamination, external 40% river and the water quality in river mouth, which are difficult to reach quality standard of water environment,
Cause, if considering pathogenic microorganism index, then the water grade in China major part river will be further compromised.Water body disease
Pathogenic microorganism pollution constitutes human health huge potential threat.And with culture fishery scale it is continuous expand and
The pollution of the continuous improvement of intensive degree, sea water culture environment and breeding water body increasingly sharpens, aquatic animal disease outburst day
Become frequent and severe, huge economic loss is caused to aquaculture industry.Water body microbiological contamination and its control increasingly obtain
To the attention of people.Therefore realized using effective ways and means extremely urgent to the real time monitoring of water environment and early warning.
Content of microorganisms test (being also known as microbial limit test) is intended to determine in finished product or non-sterilized raw material
The number of the aerobe of survival.Content of microorganisms test divides two parts to carry out：Part I is quantitative stage (microorganism meter
Number), in this stage, determine the sum and saccharomycete and the sum of mould of aerobe.Part II is qualitative stage
(microorganism that test is specified), it is intended to determine that specified microorganisms whether there is.
Through looking into, the Chinese patent of existing Patent No. CN201621066914.9《A kind of liquid state filtering extraction microorganism
Detection device》, including extraction element and reaction unit, wherein extraction element include screen pipe and filtering pump head, the bottom of screen pipe
Portion is equipped with the filter membrane that can retain microorganism, and the upper end of screen pipe is connected with the container equipped with liquid sample to be checked by taking over, filtered
Pump head is connected to the lower end of screen pipe, and filtering pump head lower end is connected by constant displacement pump, liquid suction pipe with hydrops bottle；Reaction unit bag
Include reaction tube, above-mentioned retention has the screen pipe of microorganism and containing the seal cap for detecting liquid, screen pipe is placed in reaction tube, close
Sealing cap is connected by outer urgent formula lock with the upper end of reaction tube, during reaction, is made to contain in seal cap and is detected liquid and all flowed to
In chimney filter, the filter membrane for having microorganism by retention makes sampling fully be reacted with detection liquid, and reaction unit is put into ATP fluoroscopic examinations
It is detected in instrument.Microorganism in liquid can be extracted and be detected to this detection device, and it is accurately fixed to carry out DNA in application fluorescent dye
, it is necessary to which the operation being incubated with full shading, process are comparatively laborious during amount, it is impossible to directly the content of microorganisms in liquid is carried out accurate
Quantitative detection.
The content of the invention
The technical problems to be solved by the invention are provided a kind of reasonable in design skilful for the above-mentioned state of the art
Wonderful, easy to operate separation preserves reagent filtering detection device, all kinds of detections can be coordinated to shine the use of shape instrument, by liquid to be checked
After directly extracting microorganism by present apparatus quantitative filtering, without specially treated, so that it may which the thing to be checked in direct quantitative liquid contains
Amount.
Technical solution is used by the present invention solves above-mentioned technical problem：One kind separation preserves reagent filtering detection dress
Put, including seal cap, filtering pump head and filtering reaction tube, filtering pump head lower end are connected by liquid suction pipe with hydrops bottle, its
It is characterized in that：The seal cap is compressible seal cap, and first kind reagent is contained in seal cap, and filtering reaction tube was arranged on
In filter pump head, the sealed bottom for filtering reaction tube is equipped with a removable lining sealing-plug, filters in reaction tube close to the position of bottom
Equipped with the filter membrane that can retain microbiological materials, the upper end for filtering reaction tube is connected by support ring with seal cap, support ring
Interior bottom is equipped with the sealing isolation layer for being used for placing the second class reagent, and a hollow sealing bar is arranged on support ring and seal cap
Interior, the lower seal of sealing bar is plugged on the top for being located at sealing isolation layer in support ring, is equipped with the upper end opening of sealing bar
One head cover, the top of head cover are equipped with sealant, the sharp protrusion for puncturing sealant are at least convexly equipped with head cover, sealing bar
Bottom forms the protrusion of the sealing isolation layer for puncturing support ring, when seal cap is compressed downwards, the point at least on head cover
Sharp protrusion punctures sealant, and promotes sealing bar to move down the sealing isolation layer for puncturing support ring, first kind reagent outflow and and the
The thing to be checked for flowing into after the mixing of two class reagents in filtering reaction tube and being gathered on filter membrane reacts, easy to finally by instrument
Sensor reads related data.
Further, the top inner wall of the seal cap also is provided with up-side down triangle protrusion, makes the up-side down triangle in seal cap convex
Rise to coordinate with the sharp protrusion on head cover and puncture sealant.
As an improvement, the lower part of the seal cap is the internal thread linkage section being connected with support ring, the top of seal cap
For compressible section, the top of seal cap can realize compression downwards by the control of instrument button；Head cover is located at close in ring hat shape lid
The top of bar is sealed, there is the through hole with the centre bore coaxial communication of sealing bar on head cover, sealant is bonded close using aluminum tissue paper
It is enclosed at the upper shed of head cover.
As an improvement, the top of the sealing bar is cylinder, the circular cone in downward flaring footpath is shaped in the middle part of sealing bar
Type, the middle part of sealing bar are tightly connected using female Luer mode and support ring, the protrusion of sealing bar bottom for trigone shape or
Rotate the edge of a knife.
As an improvement, being tried easy to the first kind for more rules is equipped with taper terminal in the middle part of the pyramid type of the sealing bar
The sunken inside rain line that agent flows downward.
Improve again, the support ring includes the support outer shroud being vertically connected with and support inner ring, wherein the upper end of support outer shroud
It is connected with seal cap, supports the lower end of inner ring to be connected with filtering reaction tube, the top for supporting outer shroud is the circle with sealing bar
The matched female Luer of tapered middle, supports the lower part of outer shroud to be equipped with the stop bit concave bar for being used for sealing bar clamping, supports inner ring
For the structure that top bore is big, lower part bore is small, the bottom of inner ring is supported to be equipped with the center empty slot for being used for placing the second class reagent,
Sealing isolation layer is upper and lower two layers, is sealingly disposed in the both sides up and down of center empty slot respectively, and sealing isolation layer is close using aluminium-foil paper
Envelope.
Improve again, the lower outside of the support outer shroud is circumferential to be equipped with raised screens muscle, supports in the upper inside wall of inner ring
Circumferential direction is equipped with corresponding concave card muscle groove, supports the lower part of outer shroud to be plugged in support inner ring and offsets with step surface, the two passes through
Raised screens muscle is connected with the fitted seal of concave card muscle groove.
Improve again, in the inner cavity of the support inner ring, the top of sealing isolation layer is equipped with and is used to support sealing bar to increase
The spring of leakproofness, spring are divided into high-order two, spring and low level spring, when sealing bar is compressed downwards under the compression of seal cap
High-order spring to low level spring stress point when, the first kind reagent in seal cap is flowed into support ring bottom and the second class reagent
Mix, after sealing bar continues to move down and makes the sealing isolation layer that low level spring stress punctures support inner ring bottom again, mixing
Reagent flow into filtering reaction tube in.
Further, the high-order spring is high-order low force spring, and low level spring is gravity spring.
Further, the lower outside circumferential direction of the support inner ring is convexly equipped with screens muscle, filters on the upper end inner wall of reaction tube
Corresponding circumferential groove is formed, supports the lower part of inner ring to be plugged on matching somebody with somebody by screens muscle and circumferential groove in filtering reaction tube
Connection is closed to fix.
Further, the filter membrane fixes support chip by threaded position-arresting ring with quartz sand, metal mesh sheet is positioned at
The bottom of reaction tube is filtered, the step that positioning is planted for filtering reaction tube is equipped with filtering pump head, filters the upper and lower ends of pump head
External screw thread is separately formed, the lower end for filtering pump head is connected by liquid suction pipe with hydrops bottle, filters upper end and the screw thread of pump head
It is connected after sealing cover connection by pipe with open bottles.
Finally, the second class reagent is liquid or solid.
Compared with prior art, the advantage of the invention is that：In addition to first kind reagent is set in seal cap, supporting
Ring bottom also sets up the second class reagent, can coordinate all kinds of detections shine shape instrument use, by liquid to be checked directly by this dress
Put after quantitative filtering lifts microorganism, without specially treated, so that it may which the thing content to be checked in direct quantitative liquid, is particularly applied
Fluorescent dye carries out DNA accurate quantifications, DNA and fluorescent dye can be individually positioned in Two Areas, then inserted in instrument, leads to
Cross button and realize that lucifuge is incubated, can so remove the operation that full shading is incubated from, the thing content to be checked in direct quantitative liquid；It is close
Sealing cap be designed to it is compressible, so by compression can sealing bar push, cause two class reagent flows easy to puncture sealant
Go out and mix；Design integrates the filtering reaction tube of reaction and filtering, enormously simplify structure.The present invention is reasonable in design
Operation that is ingenious, easy to operate, being incubated without full shading, just directly can carry out accurate quantification to the thing content to be checked in liquid,
Detection process is enormously simplify, has saved detection time and cost, has the characteristics that detection is sensitive, fast, accurately, the present invention is built
Fast microbiological content measuring platform has been found, the time obtained needed for result has been substantially reduced, so as to take correction rapidly
Measure efficiently solves microbial contamination test problems in liquid.
Brief description of the drawings
Fig. 1 is the structure diagram of the present invention；
Fig. 2 is the sectional view of Fig. 1；
Fig. 3 is the exploded view of Fig. 1.
Embodiment
The present invention is described in further detail below in conjunction with attached drawing embodiment.
As shown in Figures 1 to 3, a kind of separation preserves reagent filtering detection device, including seal cap 1, sealing bar 5, support ring
4th, pump head 2 and filtering reaction tube 3 are filtered, wherein filtering reaction tube 3 is arranged in filtering pump head 2, filters the bottom of reaction tube 3
Sealing is equipped with a removable lining sealing-plug 9, filters the filter membrane for being equipped with reaction tube 3 close to the position of bottom and can retaining microorganism
31, the upper end of filtering reaction tube 3 is connected by support ring 4 with seal cap 1, and bottom, which is equipped with, in support ring 4 is used to place second
The sealing isolation layer 422 of class reagent 8, seal cap 1 are compressible seal cap 1, and the lower part of seal cap 1 is to be connected with support ring 4
The internal thread linkage section connect, the top of seal cap 1 are compressible section, contain first kind reagent in seal cap 1, seal cap 1 it is upper
Portion can realize compression downwards by the control of instrument button；Sealing bar 5 is hollow structure, is arranged on support ring 4 and seal cap 1
Interior, the lower seal of sealing bar 5 is plugged on the top for being located at sealing isolation layer 422 in support ring 4, the upper end opening of sealing bar 5
Place is equipped with a head cover 6, and the upper end periphery of sealing bar 5 is circumferential to be equipped with the convex ribs 52 being connected with head cover 6, and head cover 6 is in ring hat shape lid
The top of sealing bar 5 is located at, there is the through hole with the centre bore coaxial communication of sealing bar 5, the top of head cover 6 is set on head cover 6
There is sealant 61, sealant 61 is bonded and sealed at the upper shed of head cover 6 using aluminum tissue paper, the inboard upper projection of 6 opening of head cover
It is useful for puncturing the sharp protrusion 62 of sealant 61, the top inner wall of seal cap 1 is equipped with up-side down triangle protrusion, due to seal cap 1
Be built-in with first kind reagent, first kind reagent is liquid reagent, due to the effect of atmospheric pressure, below air pressure can be more than seal cap 1
The inside pressure, causes the inside liquid smoothly to flow out, if it is desired that the inside liquid can be flowed to smoothly in following container, needs
Upper and lower air pressure is consistent, that is, puncturing 61 first kind reagent of sealant of head cover 6 could flow out, and the bottom of sealing bar 5 forms
Protrusion 51, protrusion 51 are typically irregular, and protrusion 51 can be prismatic shape or the rotation edge of a knife, for puncturing the close of support ring 4
Absciss layer 422 is insulated, and makes to form irregular status in place of rupture, is flowed out easy to liquid；When seal cap 1 is compressed downwards, sealing
The raised sharp protrusion 62 with head cover 6 of up-side down triangle in cap 1 punctures sealant 61, and promotes sealing bar 5 to move down and puncture branch
The sealing isolation layer 422 of pushing out ring 4, the outflow of first kind reagent simultaneously and after the mixing of the second class reagent 8 flows into filtering reaction tube 3 and mistake
The thing to be checked gathered on filter membrane 31 reacts, and related data is read finally by Instrument sensor；The top of sealing bar 5 is circle
Cylindricality, the middle part of sealing bar 5 are shaped to the pyramid type in downward flaring footpath, and the middle part of sealing bar 5 is using female Luer mode and branch
Pushing out ring 4 is tightly connected, and the downward easy to first kind reagent of more rules is equipped with taper terminal in the middle part of the pyramid type of sealing bar 5
The sunken inside rain line of flowing, is exactly similar to indent fluid slot；Support ring 4 includes the support outer shroud 41 being vertically connected with and support inner ring
42, wherein the upper end of support outer shroud 41 is connected with seal cap 1, supports the lower end of inner ring 42 to be connected with filtering reaction tube 3, prop up
The upper outside of support outer shroud 41 forms the external screw thread 412 being threadedly coupled with seal cap 1, support the upper inner of outer shroud 41 for
The matched female Luer in cone middle part of sealing bar 5, the lower part of support outer shroud 41 are equipped with the stop bit for 5 clamping of sealing bar
Concave bar, support inner ring 42 are the structure that top bore is big, lower part bore is small, and the bottom of support inner ring 42, which is equipped with, to be used to place second
The center empty slot 421 of class reagent 8, the second class reagent 8 can be liquid or solid, and sealing isolation layer 422 is upper and lower two layers, respectively
The both sides up and down of center empty slot 421 are sealingly disposed in, sealing isolation layer 422 is sealed using aluminium-foil paper, supports the lower part of outer shroud 41
Outside is circumferential to be equipped with raised screens muscle 411, supports circumferential direction in the upper inside wall of inner ring 42 to be equipped with corresponding concave card muscle groove 423,
The lower part of support outer shroud 41 is plugged in support inner ring 42 and offsets with step surface, the two passes through raised screens muscle 411 and concave card
The fitted seal connection of muscle groove 423；Support in the inner cavity of inner ring 42, the top of sealing isolation layer 422 is equipped with and is used to support sealing
Bar 5 is to increase the spring 7 of leakproofness, and spring 7 can be divided into high-order two, spring and low level spring (drawing does not embody), wherein high
Position spring is high-order low force spring, and low level spring is gravity spring, and the present embodiment uses a spring, when sealing bar 5 is close
Under the compression of sealing cap 1 downwards the high-order spring of compression to low level spring stress point when, the sealant 61 of head cover 6 and sealing above
Separation layer 422 is punctured, and the first kind reagent in seal cap 1 is flowed into 4 bottom of support ring and the second class reagent 8 mixes, close
After envelope bar 5 continues to move down and makes the sealing isolation layer 422 that low level spring stress punctures 42 bottom of support inner ring again, the examination of mixing
Agent is flowed into filtering reaction tube 3, and the lower outside circumferential direction of support inner ring 42 is convexly equipped with screens muscle 424, filters the upper end of reaction tube 3
Corresponding circumferential groove 32 is formed on inner wall, the lower part of support inner ring 42 is plugged in filtering reaction tube 3 by screens muscle 424
With being fixedly connected for circumferential groove 32, filter membrane 31 fixes support chip, metal by threaded position-arresting ring 33 and quartz sand
Mesh sheet is positioned at the bottom of filtering reaction tube 3, and the step that positioning is planted for 3 bottom of filtering reaction tube, mistake are equipped with filtering pump head 1
The upper and lower ends of filter pump first 1 have been separately formed external screw thread, and the lower end of filtering pump head 1 is connected by liquid suction pipe with hydrops bottle, mistake
The upper end of filter pump first 1 is connected after being connected with thread sealed closure by pipe with open bottles.
Seal cap 1 and filtering reaction tube 3 in the present embodiment can be split, and individually be preserved.
In use, first kind reagent and the second class reagent 8 first are added in seal cap 1 and 4 bottom of support ring, then will sealing
Cap 1, sealing bar 5, head cover 6 and support ring 4 link into an integrated entity；The filtering reaction tube 3 not sealed is put into filtering pump head 2, button
Tight thread sealed closure, is connected in open bottles, and the lower end of filtering pump head 2 is connected to hydrops bottle by pipe, and passes through constant displacement pump
Liquid sample to be checked is added dropwise in filtering reaction tube 3, the microorganism in liquid is trapped within the filter membrane 31 in filtering reaction tube 3
On, then sealing cover 9 is connected with filtering the bottom thread of reaction tube 3, by above-mentioned mounted support ring 4 and filtering reaction tube
3 upper end connection sealing, is placed in instrument, promotes compression seal cap 1 by instrument button, puncture the sealant 61 of head cover 6,
And promote sealing bar 5 to move down, then the upper strata sealing isolation layer 422 of support ring 5 is punctured, the outflow of first kind reagent and and the second class examination
Agent 8 mixes, and sealing bar 5 continues to move down, and punctures lower floor's sealing isolation layer 422 of support ring 5, and mix reagent flows into filtering reaction tube
React in 3 with thing to be checked, related data is read finally by Instrument sensor.
When application fluorescent dye progress DNA accurate quantifications, the operation used and be incubated with full shading can be removed from, can be by DNA
(i.e. first kind reagent) and fluorescent dye (i.e. the second class reagent) are individually positioned in Two Areas (seal cap 1 and support ring 4), so
After insert in instrument, realize that lucifuge is incubated by button, thing content to be checked that can be in direct quantitative liquid.
The above is only the preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, without departing from the technical principles of the invention, some improvements and modifications can also be made, these improvements and modifications
Also it should be regarded as protection scope of the present invention.
Claims (12)
1. one kind separation preserves reagent filtering detection device, including seal cap, filtering pump head and filtering reaction tube, pump head is filtered
Lower end is connected by liquid suction pipe with hydrops bottle, it is characterised in that：The seal cap is compressible seal cap, and seal cap includes
There is first kind reagent, filtering reaction tube is arranged in filtering pump head, and the sealed bottom for filtering reaction tube is removable lining close equipped with one
Blocking, filters the filter membrane for being equipped with reaction tube close to the position of bottom and can retaining microbiological materials, filters the upper end of reaction tube
It is connected by support ring with seal cap, bottom is equipped with the sealing isolation layer for being used for placing the second class reagent in support ring, in one
Empty sealing bar is arranged in support ring and seal cap, and the lower seal of sealing bar, which is plugged in support ring, is located at sealing isolation layer
Top, be equipped with a head cover at the upper end opening of sealing bar, the top of head cover is equipped with sealant, is at least convexly equipped with and is used on head cover
The sharp protrusion of sealant is punctured, the bottom of sealing bar forms the protrusion of the sealing isolation layer for puncturing support ring, when close
When sealing cap compresses downwards, the sharp protrusion at least on head cover punctures sealant, and promotes sealing bar to move down and puncture the close of support ring
Absciss layer is insulated, the outflow of first kind reagent is flowed into filtering reaction tube after simultaneously being mixed with the second class reagent and treated with what is gathered on filter membrane
Inspection thing reacts, easy to read related data finally by Instrument sensor.
2. separation according to claim 1 preserves reagent filtering detection device, it is characterised in that：The top of the seal cap
Inner wall also is provided with up-side down triangle protrusion, coordinates the raised sharp protrusion with head cover of the up-side down triangle in seal cap and punctures sealing
Layer.
3. separation according to claim 1 preserves reagent filtering detection device, it is characterised in that：The lower part of the seal cap
For the internal thread linkage section being connected with support ring, the top of seal cap is compressible section, and the top of seal cap can pass through instrument
Compression downwards is realized in the control of button；Head cover is located at the top of sealing bar in ring hat shape lid, have on head cover with sealing bar
The through hole of heart hole coaxial communication, sealant are bonded and sealed at the upper shed of head cover using aluminum tissue paper.
4. separation according to claim 3 preserves reagent filtering detection device, it is characterised in that：The top of the sealing bar
For cylinder, be shaped to the pyramid type in downward flaring footpath in the middle part of sealing bar, the middle part of sealing bar using female Luer mode with
Support ring is tightly connected, and the protrusion of sealing bar bottom is trigone shape or the rotation edge of a knife.
5. separation according to claim 4 preserves reagent filtering detection device, it is characterised in that：The circular cone of the sealing bar
The sunken inside rain line to flow downward easy to first kind reagent of more rules is equipped with the middle part of type in taper terminal.
6. separation according to claim 4 preserves reagent filtering detection device, it is characterised in that：The support ring includes upper
The support outer shroud of lower connection and support inner ring, wherein the upper end of support outer shroud is connected with seal cap, support inner ring lower end and
Filtering reaction tube is connected, and the top for supporting outer shroud is the matched female Luer in cone middle part with sealing bar, and support is outer
The lower part of ring is equipped with the stop bit concave bar for sealing bar clamping, supports inner ring as the structure that top bore is big, lower part bore is small, branch
The bottom for supportting inner ring is equipped with the center empty slot for being used for placing the second class reagent, and sealing isolation layer is upper and lower two layers, and sealing respectively is set
Put and sealed in the both sides up and down of center empty slot, sealing isolation layer using aluminium-foil paper.
7. separation according to claim 6 preserves reagent filtering detection device, it is characterised in that：Under the support outer shroud
It is circumferential on the outside of portion to be equipped with raised screens muscle, support circumferential direction in the upper inside wall of inner ring to be equipped with corresponding concave card muscle groove, support is outer
The lower part of ring is plugged in support inner ring and offsets with step surface, the two passes through the fitted seal of raised screens muscle and concave card muscle groove
Connection.
8. separation according to claim 6 preserves reagent filtering detection device, it is characterised in that：It is described to support the interior of inner ring
In chamber, the top of sealing isolation layer be equipped with and be used to supporting sealing bar to increase the spring of leakproofness, spring be divided into high-order spring and
Two, low level spring, when sealing bar under the compression of seal cap downwards the high-order spring of compression to low level spring stress point when, it is close
First kind reagent in sealing cap is flowed into support ring bottom and the second class reagent mixes, and sealing bar continues to move down and makes low level bullet
After spring stress punctures the sealing isolation layer of support inner ring bottom again, the reagent of mixing is flowed into filtering reaction tube.
9. separation according to claim 8 preserves reagent filtering detection device, it is characterised in that：The high position spring is height
Position low force spring, low level spring is gravity spring.
10. separation according to claim 6 preserves reagent filtering detection device, it is characterised in that：The support inner ring
Lower outside circumferential direction is convexly equipped with screens muscle, filters and corresponding circumferential groove is formed on the upper end inner wall of reaction tube, supports inner ring
Lower part be plugged on being fixedly connected by screens muscle and circumferential groove in filtering reaction tube.
11. the separation according to claims 1 to 10 any claim preserves reagent filtering detection device, its feature exists
In：The filter membrane fixes support chip by threaded position-arresting ring and quartz sand, metal mesh sheet is positioned at the bottom for filtering reaction tube
Portion, interior be equipped with of filtering pump head plant the step positioned for filtering reaction tube, and the upper and lower ends for filtering pump head have been separately formed outer spiral shell
Line, the lower end for filtering pump head are connected by liquid suction pipe with hydrops bottle, filter pump head upper end be connected with thread sealed closure after lead to
Pipe is crossed with open bottles to be connected.
12. the separation according to claims 1 to 10 any claim preserves reagent filtering detection device, its feature exists
In：The second class reagent is liquid or solid.
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| CN201711318648.3A CN107907520B (en) | 2017-12-12 | 2017-12-12 | Separation preservation reagent filters detection device |
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| CN201711318648.3A CN107907520B (en) | 2017-12-12 | 2017-12-12 | Separation preservation reagent filters detection device |
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| CN107907520B CN107907520B (en) | 2024-02-27 |
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| CN108489904A (en) * | 2018-04-28 | 2018-09-04 | 东莞德益生物医疗科技有限公司 | A kind of reaction vessel for detection |
| CN110373411A (en) * | 2019-08-22 | 2019-10-25 | 奥然科技生物(深圳)有限责任公司 | A kind of trace amount DNA extraction capsule |
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| CN107907520B (en) | 2024-02-27 |
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