CN107831309A - 一种猪伪狂犬病病毒gE蛋白抗体竞争ELISA检测试剂盒及检测方法 - Google Patents
一种猪伪狂犬病病毒gE蛋白抗体竞争ELISA检测试剂盒及检测方法 Download PDFInfo
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- CN107831309A CN107831309A CN201710991735.9A CN201710991735A CN107831309A CN 107831309 A CN107831309 A CN 107831309A CN 201710991735 A CN201710991735 A CN 201710991735A CN 107831309 A CN107831309 A CN 107831309A
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- pseudorabies virus
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/577—Immunoassay; Biospecific binding assay; Materials therefor involving monoclonal antibodies binding reaction mechanisms characterised by the use of monoclonal antibodies; monoclonal antibodies per se are classified with their corresponding antigens
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56983—Viruses
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/005—Assays involving biological materials from specific organisms or of a specific nature from viruses
- G01N2333/01—DNA viruses
- G01N2333/03—Herpetoviridae, e.g. pseudorabies virus
- G01N2333/032—Pseudorabies virus, i.e. Aujetzky virus
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Abstract
Description
编号 | 1:500 | 1:1000 | 1:2000 | 1:4000 | 1:8000 | 1:16000 | 1:32000 | 1:64000 | 阴性对照 |
1 | 1.534 | 1.324 | 1.187 | 0.980 | 0.876 | 0.765 | 0.654 | 0.532 | 0.098 |
2 | 1.221 | 1.018 | 0.998 | 0.896 | 0.765 | 0.589 | 0.498 | 0.358 | 0.087 |
3 | 1.349 | 1.234 | 0.996 | 0.879 | 0.689 | 0.601 | 0.450 | 0.325 | 0.085 |
亚类 | IgG1 | IgG2a | IgG2b | IgG3 | IgM | IgA |
OD630 | 1.325 | 0.213 | 0.267 | 0.198 | 0.387 | 0.254 |
200 | 400 | 800 | 1600 | 3200 | 6400 | 12800 | 25600 | 对照孔 | |
OD630 | 2.344 | 1.872 | 1.356 | 1.032 | 0.897 | 0.567 | 0.453 | 0.289 | 0.210 |
血清种类 | OD值 | S/N值 | 判定结果 |
PRV | 0.075 | 0.051 | 阳性 |
CSFV | 1.324 | 0.911 | 阴性 |
PCV | 1.332 | 0.917 | 阴性 |
PRRSV | 1.354 | 0.932 | 阴性 |
PEDV | 1.235 | 0.850 | 阴性 |
PRV-gB | 1.335 | 0.919 | 阴性 |
阴性对照 | 1.452 | ||
阳性对照 | 0.065 |
Claims (9)
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CN201710991735.9A CN107831309A (zh) | 2017-10-18 | 2017-10-18 | 一种猪伪狂犬病病毒gE蛋白抗体竞争ELISA检测试剂盒及检测方法 |
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CN201710991735.9A CN107831309A (zh) | 2017-10-18 | 2017-10-18 | 一种猪伪狂犬病病毒gE蛋白抗体竞争ELISA检测试剂盒及检测方法 |
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CN201710991735.9A Pending CN107831309A (zh) | 2017-10-18 | 2017-10-18 | 一种猪伪狂犬病病毒gE蛋白抗体竞争ELISA检测试剂盒及检测方法 |
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Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN111273028A (zh) * | 2020-02-25 | 2020-06-12 | 芜湖天明生物技术有限公司 | rhTSG-6直接竞争ELISA法定量检测试剂盒及其使用方法和应用 |
CN112986562A (zh) * | 2021-02-02 | 2021-06-18 | 中国农业科学院特产研究所 | 一种水貂阿留申病毒vp2蛋白抗体竞争elisa检测试剂盒及其检测方法 |
CN116790509A (zh) * | 2023-06-28 | 2023-09-22 | 中国农业科学院哈尔滨兽医研究所(中国动物卫生与流行病学中心哈尔滨分中心) | 一种分泌抗猪伪狂犬病病毒gB蛋白抗体的单克隆杂交瘤细胞株及其应用 |
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CN104459144A (zh) * | 2014-12-12 | 2015-03-25 | 河南省农业科学院 | 一种猪伪狂犬病毒强毒株和疫苗毒株鉴别检测试纸 |
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Patent Citations (5)
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Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN111273028A (zh) * | 2020-02-25 | 2020-06-12 | 芜湖天明生物技术有限公司 | rhTSG-6直接竞争ELISA法定量检测试剂盒及其使用方法和应用 |
CN112986562A (zh) * | 2021-02-02 | 2021-06-18 | 中国农业科学院特产研究所 | 一种水貂阿留申病毒vp2蛋白抗体竞争elisa检测试剂盒及其检测方法 |
CN116790509A (zh) * | 2023-06-28 | 2023-09-22 | 中国农业科学院哈尔滨兽医研究所(中国动物卫生与流行病学中心哈尔滨分中心) | 一种分泌抗猪伪狂犬病病毒gB蛋白抗体的单克隆杂交瘤细胞株及其应用 |
CN116790509B (zh) * | 2023-06-28 | 2024-02-02 | 中国农业科学院哈尔滨兽医研究所(中国动物卫生与流行病学中心哈尔滨分中心) | 一种分泌抗猪伪狂犬病病毒gB蛋白抗体的单克隆杂交瘤细胞株及其应用 |
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Inventor after: Wei Qiyong Inventor after: Ma Lei Inventor after: Dong Jun Inventor after: Dong Xiaohui Inventor after: Xu Gaoyuan Inventor after: Dan Hanbing Inventor after: Sang Xuebo Inventor after: Fan Junqing Inventor after: Jia Huiqin Inventor after: Zhou Yunduo Inventor after: Li Panpan Inventor after: Zou Weihua Inventor after: Zhang Chi Inventor before: Wei Qiyong Inventor before: Dong Jun Inventor before: Dong Xiaohui Inventor before: Xu Gaoyuan Inventor before: Dan Hanbing Inventor before: Fan Junqing Inventor before: Jia Huiqin Inventor before: Zhou Yunduo Inventor before: Li Panpan Inventor before: Zou Weihua Inventor before: Zhang Chi Inventor before: Ma Lei |
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Effective date of registration: 20180411 Address after: 430070 No. 419 hi tech 2 Road, East Lake New Technology Development Zone, Wuhan, Hubei Applicant after: WUHAN KEQIAN BIOLOGICAL CO., LTD. Applicant after: Heilongjiang Academy of Land Reclamation Sciences Address before: 430070 No. 419 hi tech two road, East Lake hi tech Development Zone, Wuhan, Hubei Applicant before: WUHAN KEQIAN BIOLOGICAL CO., LTD. |
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Application publication date: 20180323 |