CN107595737A - The composition of whitening anti-wrinkling containing Antrodia camphorata and Essence and the preparation method of Essence containing said composition - Google Patents

Abstract

The invention belongs to cosmetic technical field, and in particular to a kind of preparation method of the composition of whitening wrinkle-removing containing Antrodia camphorata and its Essence.Present invention aims at based on application on human skin Aging Mechanism, screen natural plant active component, composition is applied to the preparation of Essence with sun-proof, whitening, the composition of wrinkle-dispelling function.The composition of said composition is Antrodia camphorata extract, glycyrrhiza glabra root extract, Angelica roots, kylin draconis extract, turmeric root extract, aurantiin, has sun-proof, whitening, wrinkle-dispelling function.Said composition and Bacillus acidi lactici/soya-bean milk tunning filtrate, Sodium Hyaluronate etc. form Essence, have uvioresistant injury, suppress TYR enzyme, promote fibroblast proliferation and other effects, can skin whitening, reduce wrinkle of skin, increase skin elasticity.

Description

The composition of whitening anti-wrinkling containing Antrodia camphorata and Essence containing said composition and Essence Preparation method

Technical field

The invention belongs to cosmetic technical field, and in particular to a kind of composition of whitening anti-wrinkling containing Antrodia camphorata and containing the group The Essence of compound and the preparation method of Essence.

Background technology：

With the growth of physiological age, the continuous atrophy of application on human skin, relaxation, wrinkle of skin increase deep, color spot expansion and color Plain intensification is the continuous aging of skin.Cause the principal element of application on human skin aging for the intrinsic physiological mechanism of human senility, disease, Extraneous UV radiation, environmental pollution etc..From coming in cell aspect, the main reason for application on human skin aging for skin epidermis and The cell viability of skin corium declines.First, epidermal layer cells vigor, which declines, causes skin barrier function to reduce, under resistance Drop, while under the stimulation of ultraviolet, cell metabolism is disorderly, pigment also constantly accumulates.Secondly, skin corium cell viability declines, Fibroblasts to secrete collagen and elastin laminin in skin corium are reduced；Collagen has very strong moisture holding capacity, its Content declines, and cutis laxa, wrinkle can be caused to increase；Elastic protein fiber has certain supporting role to skin, and it reduces meeting Skin is caused to follow the string.In addition, with human senility, superficial cutaneous capillaries, which shrink, to be reduced, and local skin blood circulation is not Foot, Skin Cell nutrition and water replenishment deficiency are caused, metabolic waste can not discharge in time, and Skin Cell just slowly loses Vigor.Therefore, delay skin aging, keep skin tender white, following three aspects must be paid attention to simultaneously：1) superficial skin is improved Blood circulation, increase superficial skin nutrition and moisture supply；2) extraneous factors such as UV radiation, chemical substance, haze are reduced Injury to skin；3) fibroblast proliferation, the fibroblastic vigor of activation are promoted.

Research is found in recent decades, and various natural materials are particularly the active component isolated from plant to skin Healthcare function is nourished with good, the cosmetics of in the market more than 95% added with active components of plants or declare that plant carries It is its functional component to take thing.With the quickening of domestic aging population trend, consumer tells to the cosmetics of whitening wrinkle-removing effect Ask increasingly stronger, the market share is also continuously increased, and external famous brand enterprise has captured the market already.China has abundant Natural plant resource, using cosmetics of the active component exploitation with whitening wrinkle-removing effect of natural plants, have certain Economic value and social effect.

The content of the invention

It is an object of the present invention to be based on application on human skin Aging Mechanism, suitable natural plants functional component is screened, is formed Composition with sun-proof, whitening, wrinkle-dispelling function, and prepare Essence using said composition.Main contents of the present invention are such as Under：

A) a kind of composition of whitening wrinkle-removing containing Antrodia camphorata, its composition are as follows：

Said composition includes：

Antrodia camphorata extract extracts with glycyrrhiza glabra root extract, Angelica roots, kylin draconis extract, turmeric The one or more combination of thing, aurantiin, the composition and effect of said composition are as shown in table 1.

The composition and effect of the composition of table 1

B) a kind of composition of whitening wrinkle-removing containing Antrodia camphorata, further, the proportioning of said composition is as follows：

Antrodia camphorata extract (4%-6%), glycyrrhiza glabra root extract (30%-50%), Angelica roots (20%- 30%), kylin draconis extract (8%-12%), turmeric root extract (4%-6%), aurantiin (10%-20%).

C) a kind of composition of whitening wrinkle-removing containing Antrodia camphorata, in addition to Antrodia camphorata extract, other raw materials can pass through commercially available purchase Buy.Antrodia camphorata extract can be prepared by the following method：

(1) fresh Antrodia camphorata is placed in 40-100 DEG C of dry 0-48h in air dry oven；

(2) dry Antrodia camphorata is crushed to 10-40 mesh or so with medicinal herb grinder；

(3) take Antrodia camphorata powder to be placed in leaching vessel, 10-60 times of 20%-95% edible alcohol is added, in 40-80 DEG C Water bath sonicator Assisted Extraction 0-24h；Filter, filter residue repeats extraction once；Collect, merge filtrate, i.e. leaching liquor twice；

(4) leaching liquor is concentrated in vacuo under the conditions of 40-100 DEG C, 0- (- 0.10) MPa alcohol-free, produces Antrodia camphorata Extract (medicinal extract).

D) a kind of Essence containing Antrodia camphorata whitening anti-wrinkling composition, its composition are as follows：

The Essence is made up of functional component and auxiliary element, as shown in table 2.Its functional component is by whitening containing Antrodia camphorata (Antrodia camphorata extract, glycyrrhiza glabra root extract, Angelica roots, kylin draconis extract, turmeric carry anti-wrinkle composition Take thing, aurantiin) formed with nourishing and moisturizing composition (Bacillus acidi lactici/soya-bean milk tunning filtrate, Sodium Hyaluronate)；Aid in into Point by preservative (parahydroxyacet-ophenone, benzene oxygen alcohol), polyalcohol NMF (glycerine, butanediol, 1,2- pentanediols) and Solvent (water) forms.Because the preservative of cosmetic industry, polyalcohol NMF species are various, can not enumerate, by the work( The cosmetic formulations of effect composition and one or more auxiliary elements composition belong to scope of the invention as claimed.

The component list and effect of the Essence of table 2

E) a kind of Essence containing Antrodia camphorata whitening anti-wrinkling composition, further, its specific proportioning are as follows：

Antrodia camphorata whitening anti-wrinkling composition (8%-10%), Bacillus acidi lactici/soya-bean milk tunning filtrate (2%-3%), thoroughly Bright matter acid sodium (0.4%-0.6%), parahydroxyacet-ophenone (0.1%-0.3%), Phenoxyethanol (0.2%-1%), glycerine (1%-3%), butanediol (2%-6%), 1,2- pentanediol (2%-6%), water (surplus).

F) a kind of Essence containing Antrodia camphorata whitening anti-wrinkling composition, its preparation method are as follows：

(1) by Antrodia camphorata whitening anti-wrinkling composition (Antrodia camphorata extract, glycyrrhiza glabra root extract, angelica extract, Kylin draconis extract, turmeric root extract, aurantiin) and glycerine, butanediol, 1,2- pentanediol, parahydroxyacet-ophenone, benzene Oxygen alcohol is added in container, is heated to 60-90 DEG C, is incubated simultaneously high-speed stirred 1-30 minutes, is made the fully dispersed dissolving of extract；

(2) by Sodium Hyaluronate, enter Bacillus acidi lactici/soya-bean milk tunning filtrate and water is added in another container, stirring It is completely dissolved material；

(3) treat that (1) is down to 20-60 DEG C, pour into (2), and high-speed stirred 1-30 minutes, it is well mixed solution；

(4) by solution after vacuum takes out bubble, Essence is produced.

Embodiment

Example 1：The preparation of Antrodia camphorata extract

Fresh Antrodia camphorata 200g is placed in the Antrodia camphorata 54.0g that 50 DEG C of dry 12h must be dried in air dry oven；With Dry Antrodia camphorata is crushed to 20 mesh or so by medicinal herb grinder, is taken 50.0g Antrodia camphorata powder to be placed in 2L glass beakers, is added Enter the edible alcohols of 1.5L 80%, extraction 2h (ultrasonic power 200W, frequency 40KHz, heats temperature in ultrasonic extraction instrument Spend for 60 DEG C)；After the completion of extraction, filter, filter residue repeats extraction once；Merge filtrate twice, by filtrate Rotary Evaporators in 60 DEG C, be concentrated in vacuo under the conditions of -0.08MPa alcohol-free, produce Antrodia camphorata extract (medicinal extract) 12.0g.

Example 2：Preparation method containing Antrodia camphorata whitening anti-wrinkling composition

By Antrodia camphorata extract 8.0g, glycyrrhiza glabra root extract 160g, angelica extract 60g, kylin draconis extract 40g, turmeric root extract 20g, aurantiin 20g are added in 500mL beaker；Beaker is placed in 85 DEG C of water-bath water-baths, added 100mL edible alcohols are simultaneously stirred continuously promotion extract dispersion mixing, melt dissolving, after being well mixed, continue stirring and are incubated 1 Hour, fling to alcohol；No vinosity is treated, natural cooling is taken out, produces composition 310g.

Example 3：The preparation method of Essence containing Antrodia camphorata whitening anti-wrinkling composition

(1) by composition 60.0g (Antrodia camphorata extract 2.0g, glycyrrhiza glabra root extract 30.0g, angelica extract 10.0g, kylin draconis extract 8.0g, turmeric root extract 5.0g, aurantiin 5.0g), glycerine 16.0g, butanediol 40.0g, 1, 2- pentanediols 25.0g, parahydroxyacet-ophenone 1.60g, benzene oxygen alcohol 4.0g are added in container, 75 DEG C of insulations and high-speed stirred 15 Minute, make the fully dispersed dissolving of material；

(2) by Sodium Hyaluronate 4.0, enter Bacillus acidi lactici/soya-bean milk tunning filtrate 16.0g and water 650g and be added to separately In one container, stirring is completely dissolved material；

(3) treat that (1) is down to 40 DEG C, pour into (2), and high-speed stirred 1-30 minutes, it is well mixed solution；

(4) by solution after vacuum takes out bubble, Essence 800g is produced.

Example 4：Effect is tested and assessed and trial effect is assessed

1) UV protection evaluation

Sun-proof res divisibiles manage sun-proof and chemical sunscreen, and the principle of chemical sunscreen is that have by force using some compound molecules Strong UV absorption, by the harmful W line absorption in light and switch to molecular kinetic energy (i.e. heat energy).Therefore, ultraviolet suction can be used Receive the effect of spectroscopic methodology entry evaluation sample.The ultra light sun block lotion that certain famous brand name is chosen in experiment compares, and concrete operation method is such as Under.

It is accurate weigh the made composition 10.0mg of present example 2, Essence 150mg and state prepared by present example 3 Certain outer brand ultra light sun block lotion (SPF 15) 150mg is respectively placed in 100mL volumetric flasks, is settled to scale with 60% alcohol respectively, Shake up；Resulting solution scans the absorption spectrum of its 250nm-500nm wave band with ultraviolet-uisible spectrophotometer.Testing result is such as Shown in Fig. 1.Certain external brand product is more than 1.3 in 275nm-320nm (UVB) absorbance, but when wavelength more than 320nm it Afterwards, absorbance declines rapidly, i.e., the brand product has good protection effect to UVB, but poor to UVA protection effect, no Injuries of the UVA to skin can be effectively reduced, is easily caused tanned.And 3 made Essence of example is in the suction of 275nm-375nm wave bands Luminosity is all higher than 1, and the made composition of example 2 is also all higher than 0.8 in the absorbance of 275nm-375nm wave bands, i.e., it is of the invention Product can simultaneously effectively absorb UVA and UVB, belong to compose entirely it is sun-proof.

2) white-skinned face function is evaluated

Tyrosinase is a kind of polyphenol oxidase containing copper ion, tyrosine can be catalytically oxidized to more quinones, then pass through A series of metabolic process ultimately generates melanin, is deposited on skin, forms freckle, chloasma etc..Tyrosinase is in melanin The reaction of formation plays very important effect early stage, is the rate-limiting enzyme of human body B16 cell.Therefore, a certain concentration can be used Sample the white-skinned face function of sample is evaluated to this index of the inhibiting rate of junket ammonia enzymatic activity.Specific experiment method is as follows.

(1) reagent and instrument

Prepare following reagent：PH6.8 phosphate buffers, 2mmol/L TYRs solution, the sample of various concentrations gradient Solution (2 made composition of example, 3 made Essence of example), different solubility gradients alpha-arbutin solution (positive control), About 50U/mL tyrosinases (Tyrosinase from mushroom, SIGMA, enzyme activity >=500U/mg) solution.

Water-bath, ultraviolet specrophotometer.

(2) detect

A. enzyme activity determination (negative control)

PH6.8 phosphate buffers 1.5mL, tyrosinase solution 0.5mL are taken, mixes, test tube is placed in 37 DEG C of water and preheated 2min minutes；3mL TYR solution is added, is mixed, after 37 DEG C of water-bath 10min, measure solution is taken out immediately and exists 475nm absorbance A 1.

PH6.8 phosphate buffers 1.5mL, tyrosinase solution 0.5mL are taken, mixes, test tube is placed in 37 DEG C of water and preheated 2min minutes；3mL pH6.8 phosphate buffers are added, are mixed, after 37 DEG C of water-bath 10min, measure solution is taken out immediately and exists 475nm absorbance A 0.

Test above it is parallel three times.

B. positive control

PH6.8 phosphate buffers 1.0mL, tyrosinase solution 0.5mL, alpha-arbutin solution 0.5ml are taken, mixes, will try Pipe is placed in preheating 2min minutes in 37 DEG C of water；3mL L- tyrosine solutions are added, are mixed, after 37 DEG C of water-bath 10min, are stood Take out absorbance B1 of the measure solution in 475nm.

PH6.8 phosphate buffers 1.5mL, tyrosinase solution 0.5mL, alpha-arbutin solution 0.5ml are taken, mixes, will try Pipe is placed in preheating 2min minutes in 37 DEG C of water；3mL pH6.8 phosphate buffers are added, are mixed, after 37 DEG C of water-bath 10min, Absorbance B0 of the measure solution in 475nm is taken out immediately.

Test above it is parallel three times.

C. sample solution

PH6.8 phosphate buffers 1.0mL, tyrosinase solution 0.5mL, sample solution 0.5ml are taken, mixes, test tube is put 2min minutes are preheated in 37 DEG C of water；3mL TYR solution is added, is mixed, after 37 DEG C of water-bath 10min, is taken immediately Go out to determine absorbance C1 of the solution in 475nm.

PH6.8 phosphate buffers 1.5mL, tyrosinase solution 0.5mL, sample solution 0.5ml are taken, mixes, test tube is put 2min minutes are preheated in 37 DEG C of water；3mL pH6.8 phosphate buffers are added, are mixed, after 37 DEG C of water-bath 10min, immediately Take out absorbance C0 of the measure solution in 475nm.

Test above it is parallel three times.

(3) calculate

Inhibiting rate (%)=[(C1-C0)/(A1-A0)] × l00%.......................... (formula 1)

Wherein, A1 is the light absorption value after negative control enzymic catalytic reaction；A0 negative control blank；C1 (or B1) is addition Sample (alpha-arbutin) light absorption value after enzymic catalytic reaction afterwards；C0 (or B0) is corresponding blank；

(4) interpretation of result

Inhibiting rate of the various sample of table 3 to junket ammonia enzymatic activity

From table 3 it can be seen that 2 made composition of example has preferable inhibition to tyrosinase, its rejection ability is big About 1/5th of the inhibition of alpha-arbutin；3 made Essence of example, it also has certain suppression to tyrosinase Ability, i.e. experiment in vitro show that product of the present invention has potential white-skinned face function.

3) anti-wrinkle efficacy assessments

As stated in the Background Art, the corrugated underlying causes of skin aging are that fibroblast vigor declines, secreted glue Former albumen and elastomer albumen is reduced.Influence of the cosmetics to fibroblast proliferation is evaluated to evaluate to a certain extent Its anti-wrinkle ability.And fibroblastic proliferative conditions can be detected with mtt assay.Examination on experimental operation is as follows.

Skin fibroblasts (RAB-iCell-s010, match hundred are fervent) are taken, is digested with 0.25% pancreatin and adjusts cell Suspension density is 105/mL, is uniformly inoculated in 96 well culture plates, and 100 μ l are added dropwise per hole, and edge hole is delayed with sterile phosphate Fliud flushing is filled.It is placed in CO2 incubators after cultivating 24h, suctions out culture supernatant, the training of the sample containing various concentrations is added per hole The μ l of base (cow's serum) dilution 100 are supported, every kind of sample sets 5 concentration, and each concentration sets 6 multiple holes, while sets and be not added with sample Blank control group.After cultivating 48h respectively, culture supernatant is suctioned out, 100 μ l DMEM nutrient solutions are added per hole, and add 20 μ l Concentration is 5mg/ml MTT solution, after being incubated 4h in CO2 incubators, carefully suctions out culture supernatant, and 150 μ are added per hole L DMSO, 10min is vibrated on shaking table, crystallization is fully dissolved, surveys absorbance (A) value at 490nm with ELIASA.Experiment knot Fruit is as shown in table 4.

The influence that Antrodia camphorata extract of table 4 etc. grows to skin fibroblasts

As can be seen from the table, it is Antrodia camphorata extract, example 2 in the range of 0.01mg/mL to 0.5mg/mL in concentration Made composition can promote the propagation of skin fibroblasts, and be in concentration accordance with tolerance, i.e., with its promotion of the increase of concentration The effect of propagation is more obvious；The made Essence of the present invention equally also has the effect for promoting skin fibroblasts propagation, i.e., With anti-wrinkle function.

4) clinical test

Effect in terms of having whitening wrinkle-removing for checking composition/Essence of the invention, selects 30 volunteers to carry out Validity experiment, contrast experiment investigate.30 volunteers be the age between 40~50 years old, be randomly divided into tri- groups of A, B, C. Wherein, A groups use distilled water；B groups use the Essence made by case study on implementation 3；Using foreign countries, certain knows that the whitening of board is dispelled to C groups Wrinkle Essence.Requirement of experiment is：It is required that the volunteer of experiment is participated in for each person every day after evening face cleaning, with 1-2ml compositions (A groups For 0.5% Sodium Hyaluronate) skin of face is smeared, and gently massaging 10 minutes fully absorbs composition.Experiment is carried out altogether 4 weeks.During experiment, skin roughness is carried out to the volunteer for participating in experiment weekly and wrinkle depth is tracked test.Test The instrument used is operated for Multi probe skin detection instrument (MPA10, German CK) by professional and technical personnel.Clinical test results are such as Shown in table 5.

The clinical trial result application form of the present composition of table 5

Note 1：Whitening effect evaluates (△ E, L*a*b* value)：

Significantly：△ E >=10 and △ L ＞ 0, skin whitening effects are obvious, can with the naked eye identify；

Typically：10 ＞ △ E >=5 and △ L >=0 skin whitening effects are slight, the more difficult Direct Recognition of naked eyes；

Other：△ E ＜ 5 or △ L ＜ 0, no whitening effect or skin be dimmed or invalid data or volunteer are unsatisfied with (allergy etc.).

Note 2：Anti-wrinkle effect assessment (△ SER, skin roughness rate of change SER)：

Significantly：△ SER≤- 5%, anti-wrinkle positive effect, can with the naked eye be identified；

Typically：0 ＞ △ SER ＞ -5%, anti-wrinkle effect is slight, the more difficult Direct Recognition of naked eyes；

Other：△ SER >=0, no anti-wrinkle or invalid data or volunteer are unsatisfied with (allergy etc.).

Can be so that the composition prepared by the present invention has good whitening effect, more well-known than certain from the clinical data of table 5 The whitening effect of brand (control) is more notable；Meanwhile test data also indicates that, the composition prepared by the present invention has certain Anti-wrinkle effect.

In addition, without discovery allergy or other toxicities in this clinical test.

5) conclusion

The whitening wrinkle-removing composition containing Antrodia camphorata prepared by the present invention, have and compose the work(such as sun-proof, whitening, crease-resistant entirely Effect.The Essence developed using said composition, show that there is significant whitening, tender skin effect through assessment on probation.

The preferred embodiments of the present invention have shown and described in described above, as previously described, it should be understood that the present invention is not office It is limited to form disclosed herein, is not to be taken as the exclusion to other embodiment, and available for various other combinations, modification And environment, and can be carried out in the scope of the invention is set forth herein by the technology or knowledge of above-mentioned teaching or association area Change., then all should be in the present invention and the change and change that those skilled in the art are carried out do not depart from the spirit and scope of the present invention In the protection domain of appended claims.

Claims (8)

1. the composition of whitening anti-wrinkling containing Antrodia camphorata, it is characterised in that：Said composition includes：

Antrodia camphorata extract and glycyrrhiza glabra root extract, Angelica roots, kylin draconis extract, turmeric root extract, shaddock The one or more combination of skin glycosides.

2. the composition of whitening anti-wrinkling containing Antrodia camphorata as claimed in claim 1, it is characterised in that：The proportioning of said composition is as follows：

Antrodia camphorata extract (4%-6%), glycyrrhiza glabra root extract (30%-50%), Angelica roots (20%- 30%), kylin draconis extract (8%-12%), turmeric root extract (4%-6%), aurantiin (10%-20%).

3. the composition of whitening anti-wrinkling containing Antrodia camphorata as claimed in claim 1 or 2, it is characterised in that：Raw material Antrodia camphorata extract Processing method comprise the steps of：

Fresh Antrodia camphorata is placed in 40-105 DEG C of dry 0-48h in air dry oven；With medicinal herb grinder by dry Cinnamomum kanahirai hay Sesame is crushed to 10-40 mesh；Take Antrodia camphorata powder to be placed in leaching vessel, 10-60 times of 20%-95% edible alcohol is added, in 40- 80 DEG C of water bath sonicator Assisted Extraction 0-24h；Filter, filter residue repeats extraction once；Collect, merge filtrate, i.e. leaching liquor twice；Will Leaching liquor is concentrated in vacuo to alcohol-free under the conditions of 40-100 DEG C, 0- (- 0.10) MPa, produces Antrodia camphorata extract, i.e. medicinal extract.

4. the composition of whitening anti-wrinkling containing Antrodia camphorata as claimed in claim 3, it is characterised in that：Raw material Antrodia camphorata extract adds Work method comprises the steps of：Fresh Antrodia camphorata is placed in 50 DEG C of dry 24h in air dry oven；Will be dry with medicinal herb grinder Dry Antrodia camphorata is crushed to 20 mesh；Take Antrodia camphorata powder to be placed in leaching vessel, 30 times of 80% edible alcohol is added, in 60 DEG C of water Bathe ultrasonic wave added extraction 2h；Filter, filter residue repeats extraction once；Collect, merge filtrate, i.e. leaching liquor twice；By leaching liquor in 60 DEG C, be concentrated in vacuo under the conditions of -0.08MPa alcohol-free, produce Antrodia camphorata extract.

5. the Essence containing Antrodia camphorata whitening anti-wrinkling composition, it is characterised in that：

The Essence includes functional component and auxiliary element；Functional component includes Antrodia camphorata whitening anti-wrinkling composition and nourishing and moisturizing Composition, described Antrodia camphorata whitening anti-wrinkling composition include：Antrodia camphorata extract, glycyrrhiza glabra root extract, angelica root extraction Thing, kylin draconis extract, turmeric root extract, aurantiin；Described moisturizing composition includes Bacillus acidi lactici/soya-bean milk tunning filter Liquid, Sodium Hyaluronate；Auxiliary element includes：Preservative, polyalcohol NMF and solvent；Described Essence is by the functional component Formed with one or more auxiliary elements.

6. the Essence as claimed in claim 5 containing Antrodia camphorata whitening anti-wrinkling composition, it is characterised in that：The Essence Formula is as follows：

Antrodia camphorata whitening anti-wrinkling composition (8%-10%), Bacillus acidi lactici/soya-bean milk tunning filtrate (2%-3%), hyalomitome Sour sodium (0.4%-0.6%), parahydroxyacet-ophenone (0.1%-0.3%), Phenoxyethanol (0.2%-1%), glycerine (1%- 3%), butanediol (2%-6%), 1,2- pentanediol (2%-6%), surplus is water.

7. the Essence as claimed in claim 6 containing Antrodia camphorata whitening anti-wrinkling composition, it is characterised in that：The Essence Preparation method comprises the steps of：

(1) by Antrodia camphorata whitening anti-wrinkling composition and glycerine, butanediol, 1,2- pentanediols, parahydroxyacet-ophenone, Phenoxyethanol Add in container, be heated to 60-90 DEG C, be incubated simultaneously high-speed stirred 1-30 minutes, make the fully dispersed dissolving of extract；

(2) by Sodium Hyaluronate, enter Bacillus acidi lactici/soya-bean milk tunning filtrate and water is added in another container, stirring makes thing Material is completely dissolved；

(3) treat that (1) is down to 20-60 DEG C, pour into (2), and high-speed stirred 1-30 minutes, it is well mixed feed liquid；

(4) solution produces Essence after vacuum takes out bubble.

8. the Essence as claimed in claim 7 containing Antrodia camphorata whitening anti-wrinkling composition, it is characterised in that：The Essence Preparation method comprises the steps of：

The preparation method of the Essence comprises the steps of：

(1) by Antrodia camphorata whitening anti-wrinkling composition and glycerine, butanediol, 1,2- pentanediols, parahydroxyacet-ophenone, Phenoxyethanol Add in container, be heated to 75 DEG C, be incubated simultaneously high-speed stirred 20 minutes, make the fully dispersed dissolving of extract；

(2) by Sodium Hyaluronate, enter Bacillus acidi lactici/soya-bean milk tunning filtrate and water is added in another container, stirring makes thing Material is completely dissolved；

(3) treat that (1) is down to 50 DEG C, pour into (2), and high-speed stirred 20 minutes, it is well mixed feed liquid；

(4) by solution after vacuum takes out bubble, the Essence is produced.