CN107324609B - Method for improving aquaculture environment - Google Patents

Method for improving aquaculture environment Download PDF

Info

Publication number
CN107324609B
CN107324609B CN201710740084.6A CN201710740084A CN107324609B CN 107324609 B CN107324609 B CN 107324609B CN 201710740084 A CN201710740084 A CN 201710740084A CN 107324609 B CN107324609 B CN 107324609B
Authority
CN
China
Prior art keywords
pond
water
expanded vermiculite
parts
weight
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201710740084.6A
Other languages
Chinese (zh)
Other versions
CN107324609A (en
Inventor
侯晓明
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Hainan Xinbang Seed Industry Co ltd
Original Assignee
Hainan Changjiang Nanjiang Biotechnology Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hainan Changjiang Nanjiang Biotechnology Co Ltd filed Critical Hainan Changjiang Nanjiang Biotechnology Co Ltd
Priority to CN201710740084.6A priority Critical patent/CN107324609B/en
Publication of CN107324609A publication Critical patent/CN107324609A/en
Application granted granted Critical
Publication of CN107324609B publication Critical patent/CN107324609B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F9/00—Multistage treatment of water, waste water or sewage
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C02F3/342—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the enzymes used
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C02F3/348—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the way or the form in which the microorganisms are added or dosed
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F1/00—Treatment of water, waste water, or sewage
    • C02F1/50—Treatment of water, waste water, or sewage by addition or application of a germicide or by oligodynamic treatment
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
    • C02F2103/20—Nature of the water, waste water, sewage or sludge to be treated from animal husbandry

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Microbiology (AREA)
  • Hydrology & Water Resources (AREA)
  • Engineering & Computer Science (AREA)
  • Environmental & Geological Engineering (AREA)
  • Water Supply & Treatment (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Farming Of Fish And Shellfish (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention discloses a method for improving aquaculture environment, which mainly comprises three steps of cleaning the bottom of a pond, using electrochemical water for sterilization treatment, laying pond bottom materials at the bottom of the pond, spraying expanded vermiculite suspension, and putting compound microorganisms and compound active enzymes after water injection, wherein the three steps are one-out-of-the-way, can not be reversed, and are step-by-step and ring-by-ring buckled, so that the aquaculture environment is improved.

Description

Method for improving aquaculture environment
Technical Field
The invention relates to a method for improving aquaculture environment. Belongs to the technical field of aquaculture.
Background
The aquaculture industry of China is developed rapidly and is the only major fishery country with the aquaculture yield exceeding the fishing yield in the world. At present, the traditional aquaculture mode is mainly used in domestic aquaculture, and the traditional aquaculture mode is characterized by being a 'dense, mixed and round', and an laggard aquaculture mode which is characterized by obtaining high yield by fertilizing and feeding a large amount of fertilizers with various varieties. In order to improve the culture yield of a unit water body, high yield is pursued in a one-sided mode, water body nutrition is often excessively increased, feeding, fertilization and stocking density are excessively increased, nitrogen and phosphorus content in water is increased suddenly, transparency is reduced, water quality is deteriorated, a large amount of baits and metabolites of culture varieties in the water body are accumulated at the bottom of a pond, and pollution is serious. In the past, the natural ecology of the water body is destroyed, the aquatic animal diseases in the culture space spread, the aquatic animal diseases frequently occur, and the fishery loss is huge.
In order to prevent diseases, the situation that fish drugs are used in the whole culture period generally exists at present, but the use of the fish drugs can only be solved urgently, ecological consequences such as drug resistance and accumulation and residue in aquatic products and the environment are brought more, a vicious circle is formed, and fundamental improvement is not obtained.
Disclosure of Invention
The object of the present invention is to overcome the above mentioned disadvantages of the prior art and to provide a method for improving an aquaculture environment.
In order to achieve the purpose, the invention adopts the following technical scheme:
a method of improving an aquaculture environment comprising the steps of:
(1) draining water in the pond, cleaning the bottom of the pond, introducing clean water to the depth of 5-8 cm, sprinkling electrified water for sterilization, and draining water in the pond again;
(2) uniformly paving a layer of pond bottom material at the bottom of the pond, and then uniformly spraying a layer of expanded vermiculite suspension;
(3) slowly injecting clean water to the depth of more than 1.5m, and then putting compound microorganisms and compound active enzymes into the pond.
Preferably, the electrochemical water in the step (1) is prepared from a sodium chloride solution with the mass concentration not less than 2 per thousand by adopting electrochemical water treatment equipment, and the electrochemical water is characterized in that the mass concentration of available chlorine is 70-100 mg/L, the mass concentration of ozone is 3-4 mg/L, the pH value is 6.5-7.5, the oxidation-reduction potential is 900-1100 mV, and the hydrogen peroxide is 15-20 mg/L.
Preferably, in the step (1), cleaning the bottom of the pond comprises: and removing sludge and sundries at the bottom of the pond, and washing for 3-4 times by using clean water.
Preferably, the splashing amount of the electrochemical water in the step (1) is 2-5% of the weight of the water in the pond.
Preferably, the electrochemical water treatment time in the step (1) is 3-4 hours.
Preferably, the paving thickness of the pond bottom material in the step (2) is 8-12 cm.
Preferably, in the step (2), the pool bottom material is prepared by mixing the following components in parts by weight: 100 parts of river sand, 30-50 parts of fly ash, 10-20 parts of humus and 10-20 parts of bentonite.
Preferably, in the step (2), the spraying amount of the expanded vermiculite suspension is 2-3L/m2。
Preferably, the expanded vermiculite suspension in step (2) is prepared by the following method:
(21) heating natural vermiculite at 400-500 ℃ for 200-250 minutes, and naturally cooling to obtain expanded vermiculite;
(22) dissolving sodium humate in water, then sequentially adding ferric aluminum polysilicate and EDTA chelated copper, and uniformly stirring to obtain a nutritional agent;
(23) and (4) putting the expanded vermiculite obtained in the step (21) into the nutrient obtained in the step (22) to soak for 18-24 hours to obtain an expanded vermiculite suspension.
More preferably, in the step (22), the mass volume ratio of the sodium humate, the ferric aluminum polysilicate, the EDTA chelated copper and the water is 8-10 g: 2-3 g: 0.5-0.6 g: 100m L.
Preferably, spraying injection is adopted when clean water is injected in the step (3), and the injection time is controlled to be 5-6 hours.
Preferably, in the step (3), the adding amount of the compound microorganisms and the compound active enzymes is 1-2% and 3-4% of the weight of the water in the pond respectively.
Preferably, the preparation method of the compound microorganism is as follows: separately fermenting photosynthetic bacteria, bacillus mucilaginosus and trichoderma harzianum to obtain fermentation liquor of each strain, and then mixing and fermenting to obtain the microbial inoculum.
Further preferably, the content of the photosynthetic bacteria, bacillus mucilaginosus and trichoderma harzianum in the composite microorganism is 40-50 hundred million/g, 20-30 hundred million/g and 15-20 hundred million/g in sequence.
Further preferably, the medium used in the fermentation alone or in the fermentation mixture is selected from any one of a beef extract peptone medium, a potato medium, and an L B medium.
Further preferably, the fermentation temperature is 46-48 ℃, and the fermentation time is 20-24 hours.
Preferably, the preparation method of the compound active enzyme comprises the following steps: neutral protease, amylase, lipase and cellulase are mixed according to the mass ratio of 1: 1: 1: 1, uniformly mixing, and then mixing the mixture into a mixture with a mass ratio of 1: 1 evenly mixing montmorillonite and attapulgite.
Further preferably, the enzyme activities of the neutral protease, the amylase, the lipase and the cellulase are 12 ten thousand U/g, 5000U/g, 1 ten thousand U/g and 12 ten thousand U/g in sequence.
The invention has the beneficial effects that:
1. the method mainly comprises the three steps of cleaning the bottom of the pond, using electrochemical water for sterilization treatment, laying a pond bottom material at the bottom of the pond, spraying expanded vermiculite suspension, and throwing compound microorganisms and compound active enzymes after water injection, wherein the three steps are one-out-of-one and can not be reversed, and are carried out step by step and are buckled in a circular way, so that the aquaculture environment is improved.
2. The water in the pond is drained, the bottom of the pond is cleaned, deteriorated dirt deposited on the bottom of the pond for a long time can be removed, shallow clean water is introduced on the basis, and residual bacteria and the like adhered to the bottom of the pond can be killed by spraying electrochemical water, so that the bottom of the pond is thoroughly cleaned; then laying a new pond bottom material at the bottom of the pond and spraying expanded vermiculite suspension, so that a new pond bottom environment can be created, the expanded vermiculite suspension adsorbs the nutrient, the expanded vermiculite has a porous structure and has a similar slow release effect on the nutrient in the expanded vermiculite, so that the nutrient can be slowly released for a long time, the growth of aquatic weeds is promoted, the ecological environment in the pond is optimized, and when the nutrient is partially or completely released, the porous structure of the expanded vermiculite also provides a living environment for various microorganisms, so that the formation of microbial ecology in the pond is further promoted; and finally, the clean water is slowly injected, so that the scouring damage to the pool bottom material caused by the rapid injection of the clean water can be avoided, the compound microorganisms and the compound active enzymes are put in, the water body purification, the bacteriostasis and the like are facilitated, and the aquaculture environment is further improved.
3. The pond bottom material disclosed by the invention is composed of river sand, coal ash, humus and bentonite, has better air permeability, can avoid the growth of pathogenic microorganisms at the bottom of the pond (the breeding of a large amount of microorganisms input at the initial stage of the use of the pond can be avoided, and the breeding space of the pathogenic microorganisms can be squeezed or even cut off), is favorable for expanding the root system of the aquatic weeds, and improves the living environment at the bottom of the pond.
Detailed Description
The present invention will be further illustrated by the following examples, which are intended to be merely illustrative and not limitative.
Example 1:
a method of improving an aquaculture environment comprising the steps of:
(1) draining water in the pond, cleaning the bottom of the pond, introducing clean water to the depth of 5cm, sprinkling electrochemical water for sterilization, and draining water in the pond again;
(2) uniformly paving a layer of pond bottom material at the bottom of the pond, and then uniformly spraying a layer of expanded vermiculite suspension;
(3) slowly injecting clean water to the depth of more than 1.5m, and then putting compound microorganisms and compound active enzymes into the pond.
The electrochemical water in the step (1) is prepared from sodium chloride solution with the mass concentration not less than 2 per thousand by adopting electrochemical water treatment equipment, and the indexes are that the mass concentration of available chlorine is 70 mg/L, ozone is 3 mg/L, the pH is 6.5, the oxidation-reduction potential is 900mV, and hydrogen peroxide is 15 mg/L.
Clearing the bottom of the pond comprises: and removing sludge and impurities at the bottom of the pond, and washing for 3 times by using clean water.
The splashing amount of the electrochemical water is 2 percent of the weight of the water in the pond. The electrochemical water treatment time was 3 hours.
And (3) paving the bottom material in the step (2) to the thickness of 8 cm.
The pool bottom material is prepared by mixing the following components in parts by weight: 100 parts of river sand, 30 parts of fly ash, 10 parts of humus and 10 parts of bentonite.
The spraying amount of the expanded vermiculite suspension is 2L/m2. The expanded vermiculite suspension is prepared by the following method:
(21) heating natural vermiculite at 400 ℃ for 200 minutes, and naturally cooling to obtain expanded vermiculite;
(22) dissolving sodium humate in water, then sequentially adding ferric aluminum polysilicate and EDTA chelated copper, and uniformly stirring to obtain a nutritional agent;
(23) and (4) putting the expanded vermiculite obtained in the step (21) into the nutrient obtained in the step (22) to soak for 18 hours to obtain an expanded vermiculite suspension.
In the step (22), the mass volume ratio of the sodium humate, the ferric aluminum polysilicate, the EDTA chelated copper and the water is 8 g: 2 g: 0.5 g: 100m L.
Spraying and injecting are adopted when clean water is injected in the step (3), and the injection time is controlled to be 5 hours.
The adding amount of the compound microorganism and the compound active enzyme is 1 percent and 3 percent of the weight of the water in the pond respectively.
The preparation method of the compound microorganism comprises the following steps: separately fermenting photosynthetic bacteria, bacillus mucilaginosus and trichoderma harzianum to obtain fermentation liquor of each strain, and then mixing and fermenting to obtain the microbial inoculum. The contents of photosynthetic bacteria, bacillus mucilaginosus and trichoderma harzianum in the composite microorganism are 40 hundred million/g, 20 hundred million/g and 15 hundred million/g in sequence. The culture medium adopted during the single fermentation or the mixed fermentation is a beef extract peptone culture medium. The fermentation temperature was 46 ℃ and the fermentation time was 20 hours.
The preparation method of the compound active enzyme comprises the following steps: neutral protease, amylase, lipase and cellulase are mixed according to the mass ratio of 1: 1: 1: 1, uniformly mixing, and then mixing the mixture into a mixture with the mass ratio of 3 times of the total weight of 1: 1 evenly mixing montmorillonite and attapulgite. The enzyme activities of the neutral protease, the amylase, the lipase and the cellulase are sequentially 12 ten thousand U/g, 5000U/g, 1 ten thousand U/g and 12 ten thousand U/g.
Example 2:
a method of improving an aquaculture environment comprising the steps of:
(1) draining water in the pond, cleaning the bottom of the pond, introducing clean water to the depth of 8cm, splashing electrified water for sterilization, and draining the water in the pond again;
(2) uniformly paving a layer of pond bottom material at the bottom of the pond, and then uniformly spraying a layer of expanded vermiculite suspension;
(3) slowly injecting clean water to the depth of more than 1.5m, and then putting compound microorganisms and compound active enzymes into the pond.
The electrochemical water in the step (1) is prepared from sodium chloride solution with the mass concentration not less than 2 per thousand by adopting electrochemical water treatment equipment, and the indexes are that the mass concentration of available chlorine is 100 mg/L, ozone is 4 mg/L, the pH is 7.5, the oxidation-reduction potential is 1100mV, and hydrogen peroxide is 20 mg/L.
Clearing the bottom of the pond comprises: and removing sludge and impurities at the bottom of the pond, and washing for 4 times by using clean water.
The splashing amount of the electrochemical water is 5 percent of the weight of the water in the pond. The electrochemical water treatment time was 4 hours.
And (3) paving the bottom material in the step (2) to a thickness of 12 cm.
The pool bottom material is prepared by mixing the following components in parts by weight: 100 parts of river sand, 50 parts of fly ash, 20 parts of humus and 20 parts of bentonite.
The spraying amount of the expanded vermiculite suspension is 3L/m2. The expanded vermiculite suspension is prepared by the following method:
(21) heating natural vermiculite at 500 ℃ for 250 minutes, and naturally cooling to obtain expanded vermiculite;
(22) dissolving sodium humate in water, then sequentially adding ferric aluminum polysilicate and EDTA chelated copper, and uniformly stirring to obtain a nutritional agent;
(23) and (4) putting the expanded vermiculite obtained in the step (21) into the nutrient obtained in the step (22) to soak for 24 hours to obtain an expanded vermiculite suspension.
In the step (22), the mass volume ratio of the sodium humate, the ferric aluminum polysilicate, the EDTA chelated copper and the water is 10 g: 3 g: 0.6 g: 100m L.
Spraying and injecting are adopted when clean water is injected in the step (3), and the injection time is controlled to be 6 hours.
The adding amount of the compound microorganism and the compound active enzyme is 2 percent and 4 percent of the weight of the water in the pond respectively.
The preparation method of the compound microorganism comprises the following steps: separately fermenting photosynthetic bacteria, bacillus mucilaginosus and trichoderma harzianum to obtain fermentation liquor of each strain, and then mixing and fermenting to obtain the microbial inoculum. The contents of photosynthetic bacteria, bacillus mucilaginosus and trichoderma harzianum in the composite microorganism are 50 hundred million/g, 30 hundred million/g and 20 hundred million/g in sequence. The culture medium adopted during the single fermentation or the mixed fermentation is a potato culture medium. The fermentation temperature is 48 ℃, and the fermentation time is 24 hours.
The preparation method of the compound active enzyme comprises the following steps: neutral protease, amylase, lipase and cellulase are mixed according to the mass ratio of 1: 1: 1: 1, uniformly mixing, and then mixing the mixture into a mixture with a mass ratio of 4 times of the total weight of 1: 1 evenly mixing montmorillonite and attapulgite. The enzyme activities of the neutral protease, the amylase, the lipase and the cellulase are sequentially 12 ten thousand U/g, 5000U/g, 1 ten thousand U/g and 12 ten thousand U/g.
Example 3:
a method of improving an aquaculture environment comprising the steps of:
(1) draining water in the pond, cleaning the bottom of the pond, introducing clean water to the depth of 6cm, splashing electrified water for sterilization, and draining the water in the pond again;
(2) uniformly paving a layer of pond bottom material at the bottom of the pond, and then uniformly spraying a layer of expanded vermiculite suspension;
(3) slowly injecting clean water to the depth of more than 1.5m, and then putting compound microorganisms and compound active enzymes into the pond.
The electrochemical water in the step (1) is prepared from sodium chloride solution with the mass concentration not less than 2 per thousand by adopting electrochemical water treatment equipment, and the electrochemical water is characterized in that the mass concentration of available chlorine is 85 mg/L, the mass concentration of ozone is 4 mg/L, the pH value is 7, the oxidation-reduction potential is 1000mV, and the hydrogen peroxide is 18 mg/L.
Clearing the bottom of the pond comprises: and removing sludge and impurities at the bottom of the pond, and washing for 4 times by using clean water.
The splashing amount of the electrochemical water is 3 percent of the weight of the water in the pond. The electrochemical water treatment time was 3 hours.
And (3) paving the bottom material in the step (2) to a thickness of 10 cm.
The pool bottom material is prepared by mixing the following components in parts by weight: 100 parts of river sand, 40 parts of fly ash, 15 parts of humus and 15 parts of bentonite.
The spraying amount of the expanded vermiculite suspension is 3L/m2. The expanded vermiculite suspension is prepared by the following method:
(21) heating natural vermiculite at 450 ℃ for 220 minutes, and naturally cooling to obtain expanded vermiculite;
(22) dissolving sodium humate in water, then sequentially adding ferric aluminum polysilicate and EDTA chelated copper, and uniformly stirring to obtain a nutritional agent;
(23) and (4) putting the expanded vermiculite obtained in the step (21) into the nutrient obtained in the step (22) to soak for 20 hours to obtain an expanded vermiculite suspension.
In the step (22), the mass volume ratio of the sodium humate, the ferric aluminum polysilicate, the EDTA chelated copper and the water is 9 g: 2: 0.6 g: 100m L.
Spraying and injecting are adopted when clean water is injected in the step (3), and the injection time is controlled to be 5 hours.
The adding amount of the compound microorganism and the compound active enzyme is respectively 1.5 percent and 3.5 percent of the weight of the water in the pond.
The preparation method of the composite microorganism comprises the following steps of fermenting photosynthetic bacteria, bacillus mucilaginosus and trichoderma harzianum separately to obtain fermentation liquor of each strain, and then performing mixed fermentation to obtain the composite microorganism, wherein the content of the photosynthetic bacteria, the bacillus mucilaginosus and the trichoderma harzianum in the composite microorganism is 45 hundred million/g, 25 hundred million/g and 18 hundred million/g in sequence, a culture medium adopted during the independent fermentation or the mixed fermentation is L B culture medium, the fermentation temperature is 47 ℃, and the fermentation time is 22 hours.
The preparation method of the compound active enzyme comprises the following steps: neutral protease, amylase, lipase and cellulase are mixed according to the mass ratio of 1: 1: 1: 1, uniformly mixing, and then mixing the mixture into a mixture with the mass ratio of 3 times of the total weight of 1: 1 evenly mixing montmorillonite and attapulgite. The enzyme activities of the neutral protease, the amylase, the lipase and the cellulase are sequentially 12 ten thousand U/g, 5000U/g, 1 ten thousand U/g and 12 ten thousand U/g.
Comparative example 1
A method of improving an aquaculture environment comprising the steps of:
(1) draining water in the pond and cleaning the bottom of the pond;
(2) uniformly paving a layer of pond bottom material at the bottom of the pond, and then uniformly spraying a layer of expanded vermiculite suspension;
(3) slowly injecting clean water to the depth of more than 1.5m, and then putting compound microorganisms and compound active enzymes into the pond.
Wherein, the paving thickness of the pond bottom material in the step (2) is 10 cm.
The pool bottom material is prepared by mixing the following components in parts by weight: 100 parts of river sand, 40 parts of fly ash, 15 parts of humus and 15 parts of bentonite.
The spraying amount of the expanded vermiculite suspension is 3L/m2. The expanded vermiculite suspension is prepared by the following method:
(21) heating natural vermiculite at 450 ℃ for 220 minutes, and naturally cooling to obtain expanded vermiculite;
(22) dissolving sodium humate in water, then sequentially adding ferric aluminum polysilicate and EDTA chelated copper, and uniformly stirring to obtain a nutritional agent;
(23) and (4) putting the expanded vermiculite obtained in the step (21) into the nutrient obtained in the step (22) to soak for 20 hours to obtain an expanded vermiculite suspension.
In the step (22), the mass volume ratio of the sodium humate, the ferric aluminum polysilicate, the EDTA chelated copper and the water is 9 g: 2: 0.6 g: 100m L.
Spraying and injecting are adopted when clean water is injected in the step (3), and the injection time is controlled to be 5 hours.
The adding amount of the compound microorganism and the compound active enzyme is respectively 1.5 percent and 3.5 percent of the weight of the water in the pond.
The preparation method of the composite microorganism comprises the following steps of fermenting photosynthetic bacteria, bacillus mucilaginosus and trichoderma harzianum separately to obtain fermentation liquor of each strain, and then performing mixed fermentation to obtain the composite microorganism, wherein the content of the photosynthetic bacteria, the bacillus mucilaginosus and the trichoderma harzianum in the composite microorganism is 45 hundred million/g, 25 hundred million/g and 18 hundred million/g in sequence, a culture medium adopted during the independent fermentation or the mixed fermentation is L B culture medium, the fermentation temperature is 47 ℃, and the fermentation time is 22 hours.
The preparation method of the compound active enzyme comprises the following steps: neutral protease, amylase, lipase and cellulase are mixed according to the mass ratio of 1: 1: 1: 1, uniformly mixing, and then mixing the mixture into a mixture with the mass ratio of 3 times of the total weight of 1: 1 evenly mixing montmorillonite and attapulgite. The enzyme activities of the neutral protease, the amylase, the lipase and the cellulase are sequentially 12 ten thousand U/g, 5000U/g, 1 ten thousand U/g and 12 ten thousand U/g.
Comparative example 2
A method of improving an aquaculture environment comprising the steps of:
(1) draining water in the pond, cleaning the bottom of the pond, introducing clean water to the depth of 6cm, splashing electrified water for sterilization, and draining the water in the pond again;
(2) uniformly paving a layer of pond bottom material on the bottom of the pond;
(3) slowly injecting clean water to the depth of more than 1.5m, and then putting compound microorganisms and compound active enzymes into the pond.
The electrochemical water in the step (1) is prepared from sodium chloride solution with the mass concentration not less than 2 per thousand by adopting electrochemical water treatment equipment, and the electrochemical water is characterized in that the mass concentration of available chlorine is 85 mg/L, the mass concentration of ozone is 4 mg/L, the pH value is 7, the oxidation-reduction potential is 1000mV, and the hydrogen peroxide is 18 mg/L.
Clearing the bottom of the pond comprises: and removing sludge and impurities at the bottom of the pond, and washing for 4 times by using clean water.
The splashing amount of the electrochemical water is 3 percent of the weight of the water in the pond. The electrochemical water treatment time was 3 hours.
And (3) paving the bottom material in the step (2) to a thickness of 10 cm.
The pool bottom material is prepared by mixing the following components in parts by weight: 100 parts of river sand, 40 parts of fly ash, 15 parts of humus and 15 parts of bentonite.
Spraying and injecting are adopted when clean water is injected in the step (3), and the injection time is controlled to be 5 hours.
The adding amount of the compound microorganism and the compound active enzyme is respectively 1.5 percent and 3.5 percent of the weight of the water in the pond.
The preparation method of the composite microorganism comprises the following steps of fermenting photosynthetic bacteria, bacillus mucilaginosus and trichoderma harzianum separately to obtain fermentation liquor of each strain, and then performing mixed fermentation to obtain the composite microorganism, wherein the content of the photosynthetic bacteria, the bacillus mucilaginosus and the trichoderma harzianum in the composite microorganism is 45 hundred million/g, 25 hundred million/g and 18 hundred million/g in sequence, a culture medium adopted during the independent fermentation or the mixed fermentation is L B culture medium, the fermentation temperature is 47 ℃, and the fermentation time is 22 hours.
The preparation method of the compound active enzyme comprises the following steps: neutral protease, amylase, lipase and cellulase are mixed according to the mass ratio of 1: 1: 1: 1, uniformly mixing, and then mixing the mixture into a mixture with the mass ratio of 3 times of the total weight of 1: 1 evenly mixing montmorillonite and attapulgite. The enzyme activities of the neutral protease, the amylase, the lipase and the cellulase are sequentially 12 ten thousand U/g, 5000U/g, 1 ten thousand U/g and 12 ten thousand U/g.
Comparative example 3
A method of improving an aquaculture environment comprising the steps of:
(1) draining water in the pond, cleaning the bottom of the pond, introducing clean water to the depth of 6cm, splashing electrified water for sterilization, and draining the water in the pond again;
(2) uniformly paving a layer of pond bottom material at the bottom of the pond, and then uniformly spraying a layer of expanded vermiculite suspension;
(3) slowly injecting clean water to the depth of more than 1.5m, and then putting compound microorganisms and compound active enzymes into the pond.
The electrochemical water in the step (1) is prepared from sodium chloride solution with the mass concentration not less than 2 per thousand by adopting electrochemical water treatment equipment, and the electrochemical water is characterized in that the mass concentration of available chlorine is 85 mg/L, the mass concentration of ozone is 4 mg/L, the pH value is 7, the oxidation-reduction potential is 1000mV, and the hydrogen peroxide is 18 mg/L.
Clearing the bottom of the pond comprises: and removing sludge and impurities at the bottom of the pond, and washing for 4 times by using clean water.
The splashing amount of the electrochemical water is 3 percent of the weight of the water in the pond. The electrochemical water treatment time was 3 hours.
And (3) paving the bottom material in the step (2) to a thickness of 10 cm.
The pool bottom material is prepared by mixing the following components in parts by weight: 100 parts of river sand, 15 parts of humus and 15 parts of bentonite.
The spraying amount of the expanded vermiculite suspension is 3L/m2. The expanded vermiculite suspension is prepared by the following method:
(21) heating natural vermiculite at 450 ℃ for 220 minutes, and naturally cooling to obtain expanded vermiculite;
(22) dissolving sodium humate in water, then sequentially adding ferric aluminum polysilicate and EDTA chelated copper, and uniformly stirring to obtain a nutritional agent;
(23) and (4) putting the expanded vermiculite obtained in the step (21) into the nutrient obtained in the step (22) to soak for 20 hours to obtain an expanded vermiculite suspension.
In the step (22), the mass volume ratio of the sodium humate, the ferric aluminum polysilicate, the EDTA chelated copper and the water is 9 g: 2: 0.6 g: 100m L.
Spraying and injecting are adopted when clean water is injected in the step (3), and the injection time is controlled to be 5 hours.
The adding amount of the compound microorganism and the compound active enzyme is respectively 1.5 percent and 3.5 percent of the weight of the water in the pond.
The preparation method of the composite microorganism comprises the following steps of fermenting photosynthetic bacteria, bacillus mucilaginosus and trichoderma harzianum separately to obtain fermentation liquor of each strain, and then performing mixed fermentation to obtain the composite microorganism, wherein the content of the photosynthetic bacteria, the bacillus mucilaginosus and the trichoderma harzianum in the composite microorganism is 45 hundred million/g, 25 hundred million/g and 18 hundred million/g in sequence, a culture medium adopted during the independent fermentation or the mixed fermentation is L B culture medium, the fermentation temperature is 47 ℃, and the fermentation time is 22 hours.
The preparation method of the compound active enzyme comprises the following steps: neutral protease, amylase, lipase and cellulase are mixed according to the mass ratio of 1: 1: 1: 1, uniformly mixing, and then mixing the mixture into a mixture with the mass ratio of 3 times of the total weight of 1: 1 evenly mixing montmorillonite and attapulgite. The enzyme activities of the neutral protease, the amylase, the lipase and the cellulase are sequentially 12 ten thousand U/g, 5000U/g, 1 ten thousand U/g and 12 ten thousand U/g.
Test examples
Selecting a penaeus vannamei boone culture pond with a three-year culture history, wherein the bottom material of the pond is sand, isolating the pond into 7 small ponds with the same size, optionally selecting one small pond as a control group without any treatment, randomly treating the rest small ponds by using the methods of examples 1-3 or comparative examples 1-3, throwing seedlings for feeding 3 days after the treatment is finished, wherein the culture period is 60 days, and inspecting the water quality condition of each small pond and the culture condition of the penaeus vannamei boone after the culture period is finished, wherein the results are shown in tables 1-2.
The method for detecting the toxicity of the luminescent bacteria comprises the following steps of measuring by using UV254, namely filtering by using a 0.4-micron micropore, directly reading by using an ultraviolet spectrophotometer at a wavelength of 254nm, and measuring the luminous intensity of different water samples by using photobacterium phoremt3 freeze-dried powder of SDI company in America, wherein the luminous inhibition ratio (%) = (1-light intensity in water sample/light intensity in blank control) × 100%.
TABLE 1 Water quality in the Small ponds
Ammonia nitrogen content (mg/L) Hydrogen sulfide content (mg/L) COD concentration (mg/L) Growth amount of aquatic weeds per unit area (g/m)2） UV254 Luminescence inhibition ratio (%)
Example 1 0.15 0.08 0.12 1089 1.5 10.3
Example 2 0.15 0.07 0.13 1088 1.4 10.3
Example 3 0.12 0.06 0.1 1106 1.2 10
Comparative example 1 2.6 2.1 15.3 456 8.7 25.6
Comparative example 2 2.4 2.1 12.9 478 8.7 24.8
Comparative example 3 0.89 0.97 1.89 788 2.6 13.4
Control group 3.5 2.8 18.9 213 12.3 35.3
As can be seen from Table 1, after the ponds treated in examples 1 to 3 are cultured in one culture period, the ammonia nitrogen content, the hydrogen sulfide content and the COD concentration are low, the growth amount of aquatic weeds in unit area is high, and the UV254 and the luminescence inhibition rate are lower, so that the water quality is better and far better than that of the control group and comparative examples 1 to 3.
TABLE 2 culture conditions of Penaeus vannamei Boone
Mu yield (kg/mu) Average single weight (g)
Example 1 1003 32
Example 2 1002 32
Example 3 1012 35
Comparative example 1 228 20
Comparative example 2 413 21
Comparative example 3 876 24
Control group 132 17
As can be seen from Table 2, the small ponds treated in the examples 1 to 3 have higher yield of the penaeus vannamei per mu, and the average weight of each pond is larger and far better than that of the control group and the comparative examples 1 to 3, particularly the comparative examples 1 and the control group have the condition that a large amount of shrimps die.
Although the present invention has been described with reference to the specific embodiments, it is not intended to limit the scope of the present invention, and various modifications and variations can be made by those skilled in the art without inventive changes based on the technical solution of the present invention.

Claims (5)

1. A method of improving an aquaculture environment, comprising the steps of:
(1) draining water in the pond, cleaning the bottom of the pond, introducing clean water to the depth of 5-8 cm, sprinkling electrified water for sterilization, and draining water in the pond again;
(2) uniformly paving a layer of pond bottom material at the bottom of the pond, and then uniformly spraying a layer of expanded vermiculite suspension;
(3) slowly injecting clean water to the depth of more than 1.5m, and then putting compound microorganisms and compound active enzymes into the pond;
the electrochemical water in the step (1) is prepared from a sodium chloride solution with the mass concentration not less than 2 per thousand by adopting electrochemical water treatment equipment, and the electrochemical water is characterized in that the mass concentration of available chlorine is 70-100 mg/L, ozone is 3-4 mg/L, the pH is 6.5-7.5, the oxidation-reduction potential is 900-1100 mV, and hydrogen peroxide is 15-20 mg/L;
in the step (1), the splashing amount of the electrochemical water is 2-5% of the weight of the water in the pond;
in the step (2), the pool bottom material is prepared by mixing the following components in parts by weight: 100 parts of river sand, 30-50 parts of fly ash, 10-20 parts of humus and 10-20 parts of bentonite;
in the step (2), the spraying amount of the expanded vermiculite suspension is 2-3L/m2；
The expanded vermiculite suspension in the step (2) is prepared by the following method:
(21) heating natural vermiculite at 400-500 ℃ for 200-250 minutes, and naturally cooling to obtain expanded vermiculite;
(22) dissolving sodium humate in water, then sequentially adding ferric aluminum polysilicate and EDTA chelated copper, and uniformly stirring to obtain a nutritional agent;
(23) and (4) putting the expanded vermiculite obtained in the step (21) into the nutrient obtained in the step (22) to soak for 18-24 hours to obtain an expanded vermiculite suspension.
2. A method for improving an aquaculture environment according to claim 1 wherein in step (1) cleaning the pond bottom comprises: and removing sludge and sundries at the bottom of the pond, and washing for 3-4 times by using clean water.
3. The method for improving aquaculture environment of claim 1, wherein in step (3), the amount of the compound microorganism and the compound active enzyme is 1-2% and 3-4% of the weight of the water in the pond respectively.
4. The method of claim 1, wherein the complex microorganism is prepared by the following steps: separately fermenting photosynthetic bacteria, bacillus mucilaginosus and trichoderma harzianum to obtain fermentation liquor of each strain, and then mixing and fermenting to obtain the microbial inoculum.
5. The method for improving aquaculture environment of claim 1, wherein the preparation method of the complex active enzyme comprises the following steps: neutral protease, amylase, lipase and cellulase are mixed according to the mass ratio of 1: 1: 1: 1, uniformly mixing, and then mixing the montmorillonite and attapulgite mixture with the total weight being 3-4 times of the weight of the composite active enzyme, wherein the mass ratio of the montmorillonite to the attapulgite is 1: 1.
CN201710740084.6A 2017-08-25 2017-08-25 Method for improving aquaculture environment Active CN107324609B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201710740084.6A CN107324609B (en) 2017-08-25 2017-08-25 Method for improving aquaculture environment

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201710740084.6A CN107324609B (en) 2017-08-25 2017-08-25 Method for improving aquaculture environment

Publications (2)

Publication Number Publication Date
CN107324609A CN107324609A (en) 2017-11-07
CN107324609B true CN107324609B (en) 2020-07-14

Family

ID=60225049

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201710740084.6A Active CN107324609B (en) 2017-08-25 2017-08-25 Method for improving aquaculture environment

Country Status (1)

Country Link
CN (1) CN107324609B (en)

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102583776A (en) * 2012-02-29 2012-07-18 中国水产科学研究院淡水渔业研究中心 Compound bacteria enzyme preparation for improving cultivation watery environment and preparation method
CN104692542A (en) * 2015-02-09 2015-06-10 华南理工大学 Composite microbial oxygen producer for increasing transparency of aquaculture water and preparation method of composite microbial oxygen producer
CN105454109A (en) * 2015-12-03 2016-04-06 江苏板桥水产股份有限公司 Crab aquaculture pond processing technology
CN105645556A (en) * 2016-01-26 2016-06-08 通威股份有限公司 Method for disinfecting and sterilizing aquaculture pond water body with sterilizing electrochemical water
KR20160147096A (en) * 2015-06-11 2016-12-22 옥씨뱅크(주) Aquaculture cage having apparatus for generating nanobubble
CN106942102A (en) * 2017-01-14 2017-07-14 陆川县品养殖专业合作社 A kind of anti-river snail infects the cultural method of pipe strongylid

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102583776A (en) * 2012-02-29 2012-07-18 中国水产科学研究院淡水渔业研究中心 Compound bacteria enzyme preparation for improving cultivation watery environment and preparation method
CN104692542A (en) * 2015-02-09 2015-06-10 华南理工大学 Composite microbial oxygen producer for increasing transparency of aquaculture water and preparation method of composite microbial oxygen producer
KR20160147096A (en) * 2015-06-11 2016-12-22 옥씨뱅크(주) Aquaculture cage having apparatus for generating nanobubble
CN105454109A (en) * 2015-12-03 2016-04-06 江苏板桥水产股份有限公司 Crab aquaculture pond processing technology
CN105645556A (en) * 2016-01-26 2016-06-08 通威股份有限公司 Method for disinfecting and sterilizing aquaculture pond water body with sterilizing electrochemical water
CN106942102A (en) * 2017-01-14 2017-07-14 陆川县品养殖专业合作社 A kind of anti-river snail infects the cultural method of pipe strongylid

Also Published As

Publication number Publication date
CN107324609A (en) 2017-11-07

Similar Documents

Publication Publication Date Title
CN103058388B (en) Method for treating cyanobacteria in lakes by using loofah sponge to immobilize photosynthetic bacteria or compound microorganisms
CN104707864B (en) Compound photosynthetic bacteria preparation for enhancing phytoremediation for heavy metal pollution of soil and preparation method thereof
CN107445432A (en) A kind of riverbed sludge processing method
CN103964639A (en) Water circulating ecological system for aquaculture
JP6346982B1 (en) Method for isolating Raulterra microorganisms, method for producing plant waste treatment agent, and method for treating plant waste
CN103589672A (en) Method for preparing effective microorganism water quality purification agent
CN104845920A (en) Marine zobellella sp. and application thereof
CN103875659A (en) Pesticide decomposing agent and preparation method thereof
CN105170619A (en) Method for remediating and modifying heavy metal contaminated soil
CN104585090B (en) A kind of remodeling method of holothruian cultures cofferdam substrate
CN109179936B (en) In-situ treatment method suitable for ecological restoration of light-pollution clean pond
CN104651282A (en) Preparation method of compound photosynthetic bacterial preparation
CN102689990A (en) Method for controlling water quality in shrimp pool by using bacillus and bagasse
CN104798700A (en) Fry pond management method
WO2018190772A1 (en) Composition for aquaculture
CN107324609B (en) Method for improving aquaculture environment
CN109133356B (en) Method for removing algae by using filter feeders
CN107969357A (en) A kind of high-efficient method for cultivating of fish fry
CN103766405A (en) Microbial preparation for removing mosses on trepang aquaculture pond
CN106542651A (en) A kind of microorganism water treatment agent in culturing water and preparation method thereof
CN103667077B (en) A kind of bio-fermentation agent promoting Herba Eichhorniae to become thoroughly decomposed and preparation method thereof
CN103131688B (en) Preparation method for corncob and/or straw bearing photosynthetic bacteria and application thereof
CN109133368A (en) A kind of complex microorganism water purification agent and preparation method thereof for aquaculture
TWI358402B (en) Method for preparing nutrient liquid
CN107581121B (en) Prawn culture method using ozone and microbial technology

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
TA01 Transfer of patent application right
TA01 Transfer of patent application right

Effective date of registration: 20200616

Address after: Haiwei Town, Changjiang County, Hainan Province

Applicant after: Hainan Changjiang Nanjiang Biotechnology Co.,Ltd.

Address before: Wangsheren Licheng District of Shandong province Ji'nan 250100 North Street No. 1

Applicant before: Hou Xiaoming

GR01 Patent grant
GR01 Patent grant
TR01 Transfer of patent right
TR01 Transfer of patent right

Effective date of registration: 20221123

Address after: 572733 Sanlian Village, Haiwei Town, Changjiang Li Autonomous County, Hainan Province

Patentee after: Hainan Xinbang Seed Industry Co.,Ltd.

Address before: 572,732 Haiwei Town, Changjiang County, Hainan Province

Patentee before: Hainan Changjiang Nanjiang Biotechnology Co.,Ltd.