CN106755326A - A kind of molecular genetic marker related to duck egg-laying deseription and application - Google Patents
A kind of molecular genetic marker related to duck egg-laying deseription and application Download PDFInfo
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Abstract
The present invention relates to a kind of molecular genetic marker related to duck egg-laying deseription and application, belong to field of poultry breeding, and in particular to application of the related AMH gene G575A sites of duck egg-laying deseription as molecular genetic marker in duck production performance research.The present invention obtains one section of special DNA fragmentation as the molecular labeling related to egg duck egg-laying deseription from AMH genes, there are G575 A575 base mutations in the sequence of the molecular labeling, the 43 week old egg numbers of the mutational site and duck, 72 week old egg numbers are significantly correlated（P ＜ 0.05）, being expanded by PCR and by PCR primer sequencing and typing, selection GG genotype is egg duck.Detection method effect is significant of the invention, method simple and fast, and it is free from the influence of the external environment.
Description
Technical field
The invention belongs to field of poultry breeding, and in particular to a kind of utilization homozygosis complex gene type improves duck group's egg number
Method.
Background technology
For a long time, the cultivation of China's egg duck is more in seed selection to be carried out using families selecting mode based on group support.Egg duck colony
In low yield group be influence duck group laying rate, influence cultivation efficiency key factor, huge economic loss can be caused.Egg duck is produced
Egg number is influenceed by multiple factors such as heredity, environment, nutrition and feedings and managements, many in current egg duck production to be raised using reinforcement
Management is supported, is aided with the aggregate measures of nutrition regulation and enhancement of environment.By the above method to a certain extent, it is possible to increase egg duck
The level of laying eggs of colony.However, most basic method still carries out choice breeding by the means of genetic improvement, progressively cultivate high
The egg duck colony of product and good evenness.
Traditional breeding way is mainly selected from the phenotype of proterties, is made some progress, and particularly works as proterties
Even if hereditary basis it is relatively simple or complex but performance additive gene effect heredity when, Phenotypic Selection is highly effective
's;But the quantitative characters such as egg-laying deseription, its phenotypic character is controlled by minor-polygene, now according to phenotype offer for property
The measurement of shape genetic potential is mostly inaccurate, thus selection is often poorly efficient even invalid；And molecular labeling auxiliary choosing
Selecting can be greatly enhanced the choosing of this kind of low-heritability traits by influenceing time, the intensity of selection and the accuracy of selection
Select effect.Marker assisted selection is exactly by marking to objective trait implementation indirect selections, with the proviso that mark and objective trait
Tight association.It has been that current Application of Animal Genetic improvement engineering is taken that molecular marker assisted selection is combined with traditional breeding way
One of main method, as the basis of molecular marker assisted selection, find the mutually chain molecule of egg duck egg-laying deseription locus
Mark, to improve molecular marker assisted selection, the cultivation of laying duckses kind high provides new way and method.
SNP marker refers to the DNA sequence polymorphism caused by genome single nucleotide variations, including base transition, transversion, list
Base insertion or missing etc., are acknowledged as newest third generation DNA molecular labelings.Position according to SNP in gene can be divided
For gene coding region SNP (coding-region SNP cSNP), gene periphery SNP (perigenic SNP, pSNP) and
SNP (intergenic SNP, iSNP) three classes between gene.Wherein the SNP of gene coding region may cause the ammonia of coded by said gene
Base acid sequence changes, and then changes the advanced form of albumen, influences the function of the protein hormone of coded by said gene.
Anti- Miao Le Shi pipes hormone (anti-Mullerian hormone, AMH) is transforming growth factor β superfamily
One of (transforming growth factor β superfamily, TGF-P) member.AMH is a kind of to reproductive system
Development and its important glycoprotein, be also evaluate ovarian reserve index.In jenny, AMH to granulosa cell proliferation,
Activity of aromatizing enzyme, metakentrin acceptor have inhibitory action.It adjusts ovum original indirectly by reducing FSH and increasing inhibin
The development of cell, in Follicular growth, AMH plays main effect in the follicular recruitment stage that folliculus ovarii is developed.
In fish, mammal, AMH gene polymorphics express closely related with it.
However, research of the AMH genes in egg duck is also rarely reported, so far, there is no relevant egg duck AMH genes as
The research of the molecular labeling of duck egg-laying deseription.
The content of the invention
The purpose of the present invention is the Partial Fragment that AMH gene extron subsequences are expanded by PCR, finds mutational site, sieve
A kind of molecular labeling related to egg duck egg number is selected, and sets up batch detector methods answering as the marker assisted selection of egg duck
With.
The present invention is achieved through the following technical solutions：
Applicant is expanded by PCR, and one section of special DNA fragmentation is obtained from AMH genes as related to egg duck egg-laying deseription
Molecular labeling, its portion gene exon sequence such as sequence table SEQ ID NO:Described in 1 and Fig. 1.
In sequence table SEQ ID NO:In the sequence of 1 892bp, 1 polymorphic site is had, be mono- G → A of 575bp
Base mutation, the detection in above-mentioned site is carried out using the method for direct Sequencing.
Applicant is prepared for detecting the primer pair of above-mentioned molecular labeling base mutation, and the DNA sequence dna of the primer is as follows：
The CAGGGATAGCGGGCAGTT 3 ' of P1 forward primers 5 '（See sequence table SEQ ID NO:2）,
The TCGGCGAGAAGCAAAGC 3 ' of P2 reverse primers 5 '（See sequence table SEQ ID NO:3）.
A kind of method for preparing above-mentioned molecular labeling is applicant provided, is prepared according to following steps：
Sample populations Jingjiang duck blood STb gene is extracted, the DNA of gained is mixed and is built into DNA ponds, drawn according to reference sequences design
Thing（I.e.：Primer shown in P1 and P2）, enter performing PCR amplification, obtain the partial exon sequences of duck AMH genes, PCR primer purifying
Direct Sequencing, such as SEQ ID NO are obtained by sequence analysis afterwards:Nucleotide sequence shown in 1 and shown in Fig. 1；Wherein：Described
The exon sequence of egg duck AMH genes such as sequence table SEQ ID NO:Shown in 1.
Molecular labeling prepared by the present invention can be applied to the detection of egg duck egg-laying deseription, and described primer pair can also be applied to egg
In duck egg-laying deseription detection program.
The present invention compared with prior art, have the advantage that for：The present invention is realized carries out early stage to egg duck egg-laying deseription
Selection, detection method is quick, accurate, and is not influenceed by breeding environment condition factor.
Brief description of the drawings
Fig. 1：The genotyping result displaying of the molecular labeling of the egg duck egg-laying deseription correlation of present invention clone.
Fig. 2：The agarose gel electrophoresis figure of the AMH gene extron subsequences that PCR amplifications are obtained, swimming lane in figure：M is
DL2000Marker。
Specific embodiment
Embodiment 1
1st, the collection of duck blood sample and the extraction of DNA：
After the completion of the pedigree data of tested duck group and egg-laying test, extracted from vein under duck wing using disposable syringe
About 1ml blood, injection gently shakes up through autoclaving and in the 1.5ml centrifuge tubes equipped with the aseptic ACD anti-coagulants of 200 μ L, remembers
Record wing number, -20 DEG C save backup.
The extracting of genomic DNA extracts genomic DNA using DNA extraction kit, is operated according to kit specification, tool
Body step is taken during 10 μ L ducks blood add 1.5ml centrifuge tubes, then to adding 20 μ L Proteinase Ks and 0.5ml in centrifuge tube
Binging buffer, vortex oscillation is stored at room temperature 10 minutes after 15 seconds.By resulting solution add an adsorption column in, 12,
000 rpm is centrifuged 1 minute, abandons waste liquid.Adsorption column puts back to collecting pipe and adds 0.5ml clean buffer, 12,000 rpm from
The heart 1 minute, abandons waste liquid.Adsorption column puts back to collecting pipe and adds 0.5ml wash buffer again, and 12,000 rpm are centrifuged 1 point
Clock, abandons waste liquid, repeats 1 step time.Adsorption column is put into a clean centrifuge tube, to 65 DEG C of addition in the middle of adsorption column film
Sterilizing high purity water, after room temperature is placed 2 minutes, 12,000 rpm are centrifuged 1 minute and collect genomic DNA.
2nd, nucleotide fragments of the amplification containing site to be measured
According to duck AMH genome sequences, primer of the design comprising site sequence to be measured, using round pcr, amplification is tested duck group's
DNA, using SEQ ID NO:2 and SEQ ID NO:3 primer pairs, PCR reaction systems are 25 μ L, and wherein template DNA is 50ng,
DNTPs concentration is 200 μm of ol/L, and every primer concentration is 0.4 μm of ol/ L, 3U'sTaqArchaeal dna polymerase, plus deionized water is to total
The μ L of volume 25；PCR response procedures：94 DEG C of predegeneration 4min；Then 94 DEG C are denatured 30s, 64 DEG C of annealing 30s, 72 DEG C of extension 1min,
Totally 40 circulations；Last 72 DEG C of extensions 10min.The PCR primer of acquisition, commission elder brother Thailand is sharp（Wuhan）Bioisystech Co., Ltd surveys
Sequence.
3rd, genotype judges and association analysis
Genotype of the site in colony is detected is judged according to sequencing result.If result is as shown in figure 1, sample is homozygote
If, with regard to only one of which product peak：G peaks or A peaks；If heterozygote, 2 peaks just occur：G peaks and A peaks.
Embodiment 2（Application Example）
In the colony being made up of 320 Jingjiang ducks, to duck AMH genes different genotype and duck egg-laying deseription（Day is produced including opening
The egg number of age, 43 weeks, 72 weeks egg numbers）The detection application of association analysis is carried out.Genotype call results show, in detection
In 320 individualities, GG genotype individuals occupy the majority, and have 182 individualities, and GA genotype individuals have 90, and AA genotype individuals are most
It is few, there are 48 individualities, different genotype is as shown in table 1 with the association analysis result of proterties.
The mutational site and the week old egg number of egg duck 43, significantly correlated (the P ＜ of 72 week old egg numbers can be drawn by table 1
0.05), the gene mutation in the above-mentioned analytic explanation site exists significantly between above-mentioned proterties or extremely significantly associates.
Table 1AMHThe association analysis of gene G575A base mutations and egg-laying deseription
| Proterties | GG types（182） | AA types（48） | GA types（90） |
| Age at first laying/day | 126.74±7.03 | 132.98±8.83 | 128.62±9.93 |
| 43 weeks egg numbers/ | 155.30±8.34a | 142.18±6.44b | 145.99±6.89b |
| 72 weeks egg numbers/ | 327.38±11.75a | 286.69±22.61c | 311.74±17.96b |
Note：Indicate different Superscript letters a, b, c and represent significant difference（P<0.05）；（）Middle content representation number of individuals.
SEQ ID NO:1
<110>Academy of agricultural science animal and veterinary research institute of Hubei Province
<120>A kind of molecular genetic marker related to duck egg-laying deseription and application
<160>3
<210> 1
<211> 892
<212> DNA
<400> 1
cagggatagc gggcagttcc tgggcatgct gacccgcttc atccgccggg tgctgagccc 60
ctccagtgag ccgcccaccc agcccagctc ccaccactgg ctggacttcc agatgatgga 120
gacgctccct caccagctgc tcaacctgtc tgagacggca gcgctggagc ggctggtgca 180
gtcggaggag ccttcggtgc tgcttttccc ccaggagggc agcgccgggc tggagcagca 240
ttttggggac tggcagccag aggggaccgt gctgcagctg ctgctgggca agctgcaggc 300
agtgatccag gagctgaggg acatcccggc gttccaagcc aacatggggc ttttccagca 360
cctcctgagc ttctgctact acccgccagg gccaggcacg ggcagcgcgg gtgagcggcc 420
gcctggctct gggaagctgc acgcgctgct gctgctgaag gcgctgcaga cggtgcgagt 480
gcactggcag gagcggagga aagtcctgcg acaaaaccgc agcgcccggc accaggccca 540
ctgccggctg caggagctga ccatcgacct gcacaaccgc aagttcatcg tcatgcccac 600
cgtgtacgcc gccaacaact gcgagggtcc ctgcaagctg cccctctcca cacgtgtccc 660
cagctactac tcgcacacgg tgctgctgct gggcatgcag gagcggggct cgcccctgca 720
gcgcgctccc tgctgcgtgc ccgtccgcta ctcggaccag ctcatcatca gcgtgtccag 780
ccaggggctg gaggtgcgca agttccccaa catggtggca gaggagtgcg gctgtcggta 840
gaggctcctg cccatagccc tggagcgccc ctgcagcttt gcttctcgcc ga 892
SEQ ID NO:2
<210> 2
<211> 18
<212> DNA
<400> 2
cagggatagc gggcagtt 18
SEQ ID NO:3
<210> 3
<211> 17
<212> DNA
<400> 3
tcggcgagaa gcaaagc 17
Claims (3)
1. a kind of molecular genetic marker related to duck egg-laying deseription, it is characterised in that：The one of 892bp is obtained from AMH genes
Section specific DNA fragment has at one as the molecular genetic marker related to duck egg-laying deseription in the special DNA fragmentation
G575-A575 base mutations, are mono- base mutation of G → A of 575bp, using PCR method to comprising the special of base mutation
DNA fragmentation is expanded, and the primer used in the PCR amplifications is：
P1 forward primers 5 ' to 3 ' are CAGGGATAGCGGGCAGTT；
P2 reverse primers 5 ' to 3 ' are TCGGCGAGAAGCAAAGC.
2. a kind of application of the molecular genetic marker related to duck egg-laying deseription, it is characterised in that：Described molecular genetic marker
Can be applied to the early stage seed selection of egg duck.
3. a kind of application of the molecular genetic marker related to duck egg-laying deseription as claimed in claim 2, it is characterised in that：Egg
The early stage seed selection of duck is comprised the following steps：
1. the DNA of duck to be detected is extracted；
2. by round pcr, the DNA of duck to be measured is expanded, the product of acquisition is carried out into sequencing and typing；
3. according to the result of sequencing and typing, the duck to be detected for selecting GG genotype is required egg duck.
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107034297A (en) * | 2017-06-05 | 2017-08-11 | 江苏省家禽科学研究所 | A kind of molecular labeling related to meat duck growth traits and its application, Nucleic acid combinations and kit |
| CN108546764A (en) * | 2018-05-02 | 2018-09-18 | 浙江省农业科学院 | A kind of and the relevant molecular labeling of laying duck egg laying performance and its application in breeding |
| CN108977553A (en) * | 2018-09-05 | 2018-12-11 | 湖北省农业科学院畜牧兽医研究所 | Laying duck circular rna circ_13267 and its detection reagent, method and application |
| CN109182562A (en) * | 2018-11-27 | 2019-01-11 | 湖北省农业科学院畜牧兽医研究所 | MiRNA apla-mir-25-42 relevant to laying duck follicular development and its detection primer, mortifier and application |
| CN109628613A (en) * | 2019-01-18 | 2019-04-16 | 湖北省农业科学院畜牧兽医研究所 | A kind of molecular labeling relevant with egg quality characteristics and its application of laying eggs to laying duck |
| CN110317809A (en) * | 2019-06-27 | 2019-10-11 | 湖北省农业科学院畜牧兽医研究所 | It is a kind of regulate and control laying duck follicular development long-chain RNA Lnc-30215 and its application |
| CN111676295A (en) * | 2020-05-28 | 2020-09-18 | 浙江省农业科学院 | Research method of gene related to feed intake regulation |
| CN112501314A (en) * | 2020-12-22 | 2021-03-16 | 广东海洋大学 | Reagent for detecting genotype of SNP (single nucleotide polymorphism) locus correlated with egg laying traits of Riezou black ducks |
| CN114317776A (en) * | 2022-01-13 | 2022-04-12 | 江苏省家禽科学研究所 | Molecular marker combination related to duck egg laying traits, obtaining method and application |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20140206756A1 (en) * | 2009-07-23 | 2014-07-24 | Norbert Gleicher | Detection of infertility risk and premature ovarian aging |
-
2016
- 2016-11-28 CN CN201611061262.4A patent/CN106755326B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20140206756A1 (en) * | 2009-07-23 | 2014-07-24 | Norbert Gleicher | Detection of infertility risk and premature ovarian aging |
Non-Patent Citations (1)
| Title |
|---|
| 杨雨江等: "鸭PRL和PRLR基因多态位点及聚合基因型与其产蛋性状间的关系分析", 《第十二次全国畜禽遗传标记研讨会论文集》 * |
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| CN107034297A (en) * | 2017-06-05 | 2017-08-11 | 江苏省家禽科学研究所 | A kind of molecular labeling related to meat duck growth traits and its application, Nucleic acid combinations and kit |
| CN108546764A (en) * | 2018-05-02 | 2018-09-18 | 浙江省农业科学院 | A kind of and the relevant molecular labeling of laying duck egg laying performance and its application in breeding |
| CN108546764B (en) * | 2018-05-02 | 2021-12-24 | 浙江省农业科学院 | Molecular marker related to egg laying performance of laying ducks and application of molecular marker in breeding |
| CN108977553A (en) * | 2018-09-05 | 2018-12-11 | 湖北省农业科学院畜牧兽医研究所 | Laying duck circular rna circ_13267 and its detection reagent, method and application |
| CN109182562A (en) * | 2018-11-27 | 2019-01-11 | 湖北省农业科学院畜牧兽医研究所 | MiRNA apla-mir-25-42 relevant to laying duck follicular development and its detection primer, mortifier and application |
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| CN110317809A (en) * | 2019-06-27 | 2019-10-11 | 湖北省农业科学院畜牧兽医研究所 | It is a kind of regulate and control laying duck follicular development long-chain RNA Lnc-30215 and its application |
| CN111676295A (en) * | 2020-05-28 | 2020-09-18 | 浙江省农业科学院 | Research method of gene related to feed intake regulation |
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