CN106665571A - Biological agent capable of improving drought resistance of turf grass and preparation method and application of biological agent - Google Patents
Biological agent capable of improving drought resistance of turf grass and preparation method and application of biological agent Download PDFInfo
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- CN106665571A CN106665571A CN201611053262.XA CN201611053262A CN106665571A CN 106665571 A CN106665571 A CN 106665571A CN 201611053262 A CN201611053262 A CN 201611053262A CN 106665571 A CN106665571 A CN 106665571A
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- 238000002360 preparation method Methods 0.000 title claims abstract description 32
- 244000025254 Cannabis sativa Species 0.000 title claims abstract description 25
- 239000003124 biologic agent Substances 0.000 title abstract 6
- GGLZPLKKBSSKCX-YFKPBYRVSA-N L-ethionine Chemical compound CCSCC[C@H](N)C(O)=O GGLZPLKKBSSKCX-YFKPBYRVSA-N 0.000 claims abstract description 63
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 32
- 229920001213 Polysorbate 20 Polymers 0.000 claims abstract description 12
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 claims abstract description 12
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 claims abstract description 12
- 238000003756 stirring Methods 0.000 claims abstract description 7
- 239000002904 solvent Substances 0.000 claims abstract description 3
- 238000003760 magnetic stirring Methods 0.000 claims description 6
- ZIHOJZVODVUYHC-UHFFFAOYSA-N NC(C(=O)O)CC.[S] Chemical compound NC(C(=O)O)CC.[S] ZIHOJZVODVUYHC-UHFFFAOYSA-N 0.000 claims 1
- LURVIJVAAIIEQT-RGMNGODLSA-N [S].C(CC)N[C@@H](CCO)C(=O)O Chemical compound [S].C(CC)N[C@@H](CCO)C(=O)O LURVIJVAAIIEQT-RGMNGODLSA-N 0.000 claims 1
- 241000196324 Embryophyta Species 0.000 abstract description 11
- 230000008641 drought stress Effects 0.000 abstract description 4
- 238000005303 weighing Methods 0.000 abstract 1
- 239000007921 spray Substances 0.000 description 33
- 238000000034 method Methods 0.000 description 23
- 239000000463 material Substances 0.000 description 13
- 230000003204 osmotic effect Effects 0.000 description 10
- 238000011282 treatment Methods 0.000 description 10
- 241000234643 Festuca arundinacea Species 0.000 description 9
- 239000004576 sand Substances 0.000 description 9
- 230000000694 effects Effects 0.000 description 8
- 239000012153 distilled water Substances 0.000 description 7
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 6
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 6
- 102000004190 Enzymes Human genes 0.000 description 5
- 108090000790 Enzymes Proteins 0.000 description 5
- 239000002689 soil Substances 0.000 description 5
- 102000016938 Catalase Human genes 0.000 description 4
- 108010053835 Catalase Proteins 0.000 description 4
- 102000003992 Peroxidases Human genes 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- 108040007629 peroxidase activity proteins Proteins 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- 244000052363 Cynodon dactylon Species 0.000 description 3
- 239000003963 antioxidant agent Substances 0.000 description 3
- 230000003078 antioxidant effect Effects 0.000 description 3
- 238000000889 atomisation Methods 0.000 description 3
- 239000004202 carbamide Substances 0.000 description 3
- 235000013877 carbamide Nutrition 0.000 description 3
- 230000003247 decreasing effect Effects 0.000 description 3
- 230000006806 disease prevention Effects 0.000 description 3
- 229910052757 nitrogen Inorganic materials 0.000 description 3
- 238000003044 randomized block design Methods 0.000 description 3
- 238000012216 screening Methods 0.000 description 3
- 238000005507 spraying Methods 0.000 description 3
- 239000000758 substrate Substances 0.000 description 3
- 238000005406 washing Methods 0.000 description 3
- WSMYVTOQOOLQHP-UHFFFAOYSA-N Malondialdehyde Chemical compound O=CCC=O WSMYVTOQOOLQHP-UHFFFAOYSA-N 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 210000000170 cell membrane Anatomy 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 239000003643 water by type Substances 0.000 description 2
- QWWVBNODQCWBAZ-WHFBIAKZSA-N (2r)-2-amino-3-[(2r)-2-carboxy-2-(methylamino)ethyl]sulfanylpropanoic acid Chemical compound CN[C@H](C(O)=O)CSC[C@H](N)C(O)=O QWWVBNODQCWBAZ-WHFBIAKZSA-N 0.000 description 1
- 108060006004 Ascorbate peroxidase Proteins 0.000 description 1
- 241000209504 Poaceae Species 0.000 description 1
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 1
- 102000019197 Superoxide Dismutase Human genes 0.000 description 1
- 108010012715 Superoxide dismutase Proteins 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 238000013019 agitation Methods 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 208000003464 asthenopia Diseases 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000006735 deficit Effects 0.000 description 1
- 239000000428 dust Substances 0.000 description 1
- 239000003792 electrolyte Substances 0.000 description 1
- 230000008030 elimination Effects 0.000 description 1
- 238000003379 elimination reaction Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 238000010353 genetic engineering Methods 0.000 description 1
- 208000006278 hypochromic anemia Diseases 0.000 description 1
- 239000003621 irrigation water Substances 0.000 description 1
- 230000028514 leaf abscission Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000000116 mitigating effect Effects 0.000 description 1
- 239000003471 mutagenic agent Substances 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 238000005325 percolation Methods 0.000 description 1
- 150000002978 peroxides Chemical class 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 229910052717 sulfur Inorganic materials 0.000 description 1
- 239000011593 sulfur Substances 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 238000012549 training Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
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- Life Sciences & Earth Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a biological agent capable of improving the drought resistance of turf grass and a preparation method and application of the biological agent. Ethionine is applied to improvement of the drought resistance of the turf grass. The biological agent capable of improving the drought resistance of the turf grass contains 50-350mg/L of L-ethionine on the basis of water as a solvent. The preparation method of the biological agent capable of improving the drought resistance of the turf grass comprises the following steps of (1) adding weighed ethionine to water, and stirring the ethionine on a magnetic stirrer for fully dissolving; and (2) weighing tween-20, putting the tween-20 into the solution prepared in the step (1) to prepare the solution with the volume ratio of 0.1%, and fully stirring on the magnetic stirrer evenly. The drought resistance of the turf grass can be improved by the ethionine; the water holding capacity of a plant can be strengthened by the turf grass sprayed with the ethionine in a drought stress environment; and relatively high turf grass quality is maintained.
Description
Technical field
The invention belongs to sod production and management technique field, be related to improve the biological preparation of turf grass drought resistance and
Its preparation method and application.
Background technology
Lawn is mitigating city haze, absorption toxic and harmful, is reducing airborne dust, conserve water and soil, alleviate visual fatigue etc.
Aspect has played important function.Ended for the end of the year 2014, the Chinese city greenery patches gross area has reached 252.79 ten thousand hm2(China statistical
Yearbook, 2015), and green coverage is continuing to grow at top speed.However, China's water resource critical shortage, urban afforestation is special with water
Be lawn irrigation water and productive life water contradiction it is also outstanding day by day, make turfgrass be subject to the adverse effect of water deficit,
Including symptoms such as leaf abscission, wilting, chlorosis, turf quality declines, and particularly in arid and semi-arid area, these impacts are more bright
It is aobvious.Even if in humid region, period of short-term drought can also affect the landscape and ecological functions of turfgrass.Therefore, turfgrass is effectively improved
Drought resistance is to solve urban lawn greening problem demanding prompt solution, the development of China's Lawn Industry is may advantageously facilitate, with huge
Economic worth and vast potential for future development.
At present, improving turf grass drought resistance can adopt the methods such as wild species introduction and acclimatization, genetic engineering breeding, but these
Method is time-consuming long, and high cost needs to put into a large amount of manpower and materials.In addition to above measure, it is one to squirt biological preparation by external source
Plant the important channel of quick, effective and easy to spread raising turf grass drought resistance.
Ethionine (ethionine) is a kind of sulfur-containing amino acid, and molecular weight 163.24 is used relatively in plant field
Many is to carry out screening mutant as mutagenic agent, but the drought resistance aspect for improving turfgrass as biological preparation yet there are no report
Road.
The content of the invention
The purpose of the present invention is for the deficiencies in the prior art, there is provided the new opplication of ethionine.
It is a further object of the present invention to provide a kind of biological preparation for improving turf grass drought resistance.
It is yet another object of the invention to provide the preparation method of said preparation.
The purpose of the present invention can be achieved through the following technical solutions：
Application of the ethionine in turf grass drought resistance is improved.
A kind of biological preparation for improving turf grass drought resistance, with water as solvent, includes 25-350mg/L ethionines, preferably
Containing 50-300mg/L ethionines；Further preferably 50mg/L ethionines or 250mg/L ethionines.To season type
Grass, ethionine optium concentration is 50mg/L, is 250mg/L to cool-season grasses optium concentration.
Wherein, described ethionine is L- ethionines.
Further preferably containing 1 ‰ tween 20 in described preparation.
The preparation method of the biological preparation for improving turf grass drought resistance of the present invention, comprises the steps of：
1) weigh ethionine to be added to the water, the stirring on magnetic stirring apparatuss is allowed to fully dissolving；
2) weigh tween 20, be put into step 1) prepare solution in, the solution of volume ratio 1 ‰ is configured to, in magnetic agitation
It is sufficiently stirred for being allowed to uniform on device.
Application of the biological preparation of the present invention in turf grass drought resistance is improved.
Beneficial effect：
Ethionine can improve the drought resistance of turfgrass, under drought stress environment, spray the turfgrass of ethionine
Plant moisture holding capacity can be strengthened, higher turf quality is maintained.Foliage-spray ethionine inhibits the mistake of turfgrass film fat
Oxidation, maintains the stability and integrity of higher cell membrane, is embodied in relatively low EL and MDA contents；And make CAT
Significantly raise with the plant under APX activity ratios individually arid.
Description of the drawings
Fig. 1 experiment process 15 days, relief capabilities of the variable concentrations ethionine to cold-season turfgrass drought stress
Relief capabilities of Fig. 2 variable concentrations ethionine to warm season turf drought stress
Soil moisture content under Fig. 3 different disposals
Control is control, and ethionine is ethionine, and Drought is Osmotic treatment, similarly hereinafter
The phenotypic map of Festuca Arundinacea under Fig. 4 Osmotic treatments
Impact of Fig. 5 different disposals to turf quality
Note：Vertical line represents least significant difference LSD between different disposal in figure, and significant level is P=0.05, similarly hereinafter.
Impact of Fig. 6 different disposals to Festuca Arundinacea leaf water content
Impact of Fig. 7 different disposals to turbine blades in electrochemical matter percolation ratio and mda content
Different lower cases represent significant difference between different disposal on the same day, similarly hereinafter.
Impact of Fig. 8 different disposals to antioxidant enzyme activities in leaves
Specific embodiment
Embodiment 1
1st, the preparation of biological preparation
1) weigh ethionine to be added to the water, the stirring on magnetic stirring apparatuss is allowed to fully dissolving；
2) tween 20 (Tween-20) is weighed, in being put into 1) solution, the solution of 1 ‰ (volume ratios) is configured to, is stirred in magnetic force
To mix be sufficiently stirred on device and be allowed to uniform.
2nd, materials and methods
Material to be tested is cold-season turfgrass Festuca Arundinacea ' Arid3 ' (Festuca arundinacea cv. ' Arid3 '),
The method nourished and generated is taken to carry out material culture.Take on June 3rd, 2015 and generate within 2 years ripe tall grass skin bit, transplanting
To in the pvc pipe for filling full sand substrate (the diameter 11cm of pipe, height 50cm), sand is rough sand in the washing of the Changjiang river.Turfgrass is in temperature
Preculture in the booth of room, waters 1 time per 2d, is once pruned according to 1/3 principle every 2d, keeps height to be about 5cm.Pre- training
Foster period applies carbamide once (nitrogen content >=46.3%), notes pre- disease prevention.Hygrothermograph is installed in booth, record it is daily most
High temperature, the lowest temperature and relative humidity.
Material preculture 60 days, to growing fine, when uniform color is consistent test process are started.Ethionine set 6 it is dense
Degree gradient carries out concentration screening, respectively 0mg/L, 50mg/L, 100mg/L, 150mg/L, 200mg/L, 250mg/L, 300mg/
L、350mg/L.Each concentration is processed and is repeated 5 times.0d starts to spray, and sprays once every 5d later, coprocessing 15 days.
Using randomized block design, 8 process are designed, be respectively:
(1) arid D：Foliage-spray 10ml distilled water；
(2) arid D+ethionine ethionines：Foliage-spray 10ml C1(50mg/L) ethionine；
(3) arid D+ethionine ethionines：Foliage-spray 10ml C2(100mg/L) ethionine；
(4) arid D+ethionine ethionines：Foliage-spray 10ml C3(150mg/L) ethionine；
(5) arid D+ethionine ethionines：Foliage-spray 10ml C4(200mg/L) ethionine；
(6) arid D+ethionine ethionines：Foliage-spray 10ml C5(250mg/L) ethionine；
(7) arid D+ethionine ethionines：Foliage-spray 10ml C6(300mg/L) ethionine；
(8) arid D+ethionine ethionines：Foliage-spray 10ml C7(350mg/L) ethionine.
Osmotic treatment group (1)-(8) stop watering completely, and when 0d, 5d, 10d, 15d is tested note of taking pictures is carried out
Record, often pipe sprays equal-volume distilled water or ethionine solution 10ml, requires that atomization is good when spraying, and sprinkling is uniform, and water droplet is not
Into under plume.
3rd, result
As seen from Figure 1, during Osmotic treatment 15 days, the apparent mass of Festuca Arundinacea ' Arid3 ' occurs under different disposal
It is decreased obviously, but the process of foliage-spray 250mg/L ethionines has preferable level ground appearance quality, holds green character best.
Embodiment 2
1st, the preparation of biological preparation
1) ethionine is weighed, the stirring on magnetic stirring apparatuss is allowed to fully dissolving；
2) tween 20 (Tween-20) is weighed, in being put into 1) solution, the solution of 1 ‰ (volume ratios) is configured to, is stirred in magnetic force
To mix be sufficiently stirred on device and be allowed to uniform.
2nd, materials and methods
Material to be tested is that (Cynodon dactylon cv. ' Tifway ', take nutrition numerous to warm season turf Bermuda grass
The method grown carries out material culture.In on July 4th, 2015 to the aerial stem of plant cuttage for gathering in the pvc pipe for filling full sand substrate
(the diameter 11cm of pipe, height 50cm).Sand is rough sand in the washing of the Changjiang river.Turfgrass preculture in warmhouse booth, per 2d 1 is watered
It is secondary, once pruned according to 1/3 principle every 2d, keep height to be about 5cm.Carbamide once (nitrogen content is applied during preculture
>=46.3%), note pre- disease prevention.Hygrothermograph is installed in booth, the daily highest temperature, the lowest temperature and relatively wet is recorded
Degree.
Material preculture 60 days, to growing fine, when uniform color is consistent test process are started.Ethionine sets, and 4 dense
Degree gradient carries out concentration screening, respectively 0mg/L, 25mg/L, 50mg/L, 150mg/L.Each concentration is processed and is repeated 5 times.0d is opened
Beginning sprays, and sprays once every 5d later, coprocessing 25 days.
Using randomized block design, 4 process are designed, be respectively:
(1) arid D：Foliage-spray steams 10ml distilled water；
(2) arid D+ethionine ethionines：Foliage-spray 10ml C1(25mg/L) ethionine；
(3) arid D+ethionine ethionines：Foliage-spray 10ml C2(50mg/L) ethionine；
(4) arid D+ethionine ethionines：Foliage-spray 10ml C3(150mg/L) ethionine.
Osmotic treatment group (1)-(4) stop watering completely, and when 0d, 5d, 10d, 15d is tested note of taking pictures is carried out
Record, often pipe sprays equal-volume distilled water or ethionine solution 10mL, requires that atomization is good when spraying, and sprinkling is uniform, and water droplet is not
Into under plume.Matched group replaces medicinal liquid foliage-spray with equivalent distilled water.
3rd, result
As seen from Figure 2, during Osmotic treatment 20 days, under the apparent mass of Bermuda grass occurs substantially under different disposal
Drop, but the process of foliage-spray 50mg/L ethionines has preferable level ground appearance quality, holds green character best.
Embodiment 3
1st, the preparation of biological preparation
1) ethionine is weighed, the stirring on magnetic stirring apparatuss is allowed to fully dissolving；
2) tween 20 (Tween-20) is weighed, in being put into 1) solution, the solution of 1 ‰ (volume ratios) is configured to, is stirred in magnetic force
To mix be sufficiently stirred on device and be allowed to uniform.
2nd, materials and methods
Material to be tested is Festuca Arundinacea ' Arid3 ' (F.arundinacea cv. ' Arid3 '), takes the method nourished and generated
Carry out material culture.Take on June 3rd, 2,015 2 years and generate ripe tall grass skin bit, be transplanted to and fill full sand substrate
In pvc pipe (the diameter 11cm of pipe, height 50cm).Sand is rough sand in the washing of the Changjiang river.Turfgrass preculture in warmhouse booth, often
2d waters 1 time, is once pruned according to 1/3 principle every 2d, keeps height to be about 5cm.Carbamide is applied during preculture once
(nitrogen content >=46.3%), notes pre- disease prevention.Hygrothermograph is installed in booth, the daily highest temperature, the lowest temperature is recorded
And relative humidity.
Material preculture 60 days, to growing fine, when uniform color is consistent test process are started.Ethionine concentration is adopted
The 250mg/L that embodiment 1 is filtered out.Each process is repeated 5 times, coprocessing 25 days.
Using randomized block design, 4 process are designed, be respectively:
(1) Control is compareed:Normally water；
(2) Control+ethionine ethionines are compareed:Normally water, while foliage-spray 10ml 250mg/L second
Methyllanthionine；
(3) arid D:Foliage-spray 10ml distilled water；
(4) arid D+ethionine ethionines:Foliage-spray 10ml 250mg/L ethionines.
Osmotic treatment group (1)-(4) stop watering completely, are taken pictures when test 0d, 5d, 10d, 18,25d
Record, evaluate turf quality (Turf quality, TQ), and determine soil water content (Soil water content,
SWC), leaf r elative water content (Relative water content, RWC), Electrolytic leakage (Electrolyte
leakage,EL).Malonaldehyde (MDA) content and activities of antioxidant enzymes, including superoxide dismutase are determined within 25 days in experiment process
Enzyme (SOD), peroxidase (POD), catalase (CAT) and ascorbate peroxidase enzyme (APX).Often pipe is sprayed
Volume distilled water or ethionine solution 10ml, require that atomization is good when spraying, sprinkling is uniform, and water droplet is not under plume.
3rd, result
From figure 3, it can be seen that either normally watering still under arid situation, SWC is without notable between two process
Difference, illustrates that the soil moisture situation for compareing positive Osmotic treatment is respectively at same level.
As can be seen from Figure 4 and Figure 5, Osmotic treatment makes Turf Quality of Tall Fescue constantly reduce, when coercing 18 days, lawn matter
Amount drastically drops to the 30% of control, but foliage-spray ethionine maintains higher turf quality, under its turf quality
The 13% of control is reduced to, and is statistically not significantly different from.When processing 25 days, two controls all maintain 7.8-8, but coerce
Urgent process makes turf quality be remarkably decreased, and arid makes 45% that it drops to control, and foliage-spray ethionine makes grass
Level ground Quality Down is the 24% of control, illustrates that foliage-spray ethionine can significantly improve the drought resisting energy of cold-season turfgrass
Power.
Leaf water content RWC is one of important indicator of reaction plant drought resistance, directly represents the energy that blade preserves moisture
Power.From fig. 6, it can be seen that individually Osmotic treatment 5 days when, Festuca Arundinacea RWC is just remarkably decreased, but in whole experiment process
In, foliage-spray ethionine makes RWC be significantly higher than the plant of independent arid, illustrates that ethionine can improve plant water holding
Ability.
Electrolytic leakage EL is capable of the stability and integrity of reacting cells film, and MDA can embody the peroxide of film fat
Change situation.From figure 7 it can be seen that with the prolongation of dry spell, degree of drought constantly increases, EL and MDA is significantly raised, and leaf
Face sprays the peroxidating that ethionine inhibits film fat, maintains the stability and integrity of higher cell membrane, concrete manifestation
For relatively low EL and MDA contents.
Arid can cause the accumulation of active oxygen (ROS), and plant is self by startup Antioxidant Enzyme Systems elimination activity
Destruction of the oxygen to film, so as to improve the drought-resistant ability of plant.From figure 8, it is seen that with the prolongation of experiment periods, independent arid is drawn
The notable rising of SOD and POD activity is played, foliage-spray ethionine does not affect the activity of SOD enzymes, but makes POD enzymatic activitys big
Width rises, and is 1.43 times under independent arid situation；Meanwhile, under arid situation, foliage-spray ethionine makes CAT and APX
Plant under the independent arid of activity ratio significantly raises.
Claims (8)
1. application of the ethionine in turf grass drought resistance is improved.
2. it is a kind of improve turf grass drought resistance biological preparation, it is characterised in that with water as solvent, include 25-350mg/L second sulfur
Propylhomoserin.
3. biological preparation according to claim 2, it is characterised in that containing 50-300mg/L ethionines.
4. biological preparation according to claim 3, it is characterised in that containing 50mg/L or 250mg/L ethionines.
5. the biological preparation according to any one of claim 2-4, it is characterised in that described ethionine is L- second sulfur
Amino butyric acid.
6. the biological preparation according to any one of claim 2-4, it is characterised in that also containing 1 ‰ in described preparation
Tween 20.
7. described in claim 2-4 raising turf grass drought resistance biological preparation preparation method, it is characterised in that comprising with
Lower step：
1) weigh ethionine to be added to the water, the stirring on magnetic stirring apparatuss is allowed to fully dissolving；
2) weigh tween 20, be put into step 1) prepare solution in, the solution of volume ratio 1 ‰ is configured to, on magnetic stirring apparatuss
It is sufficiently stirred for being allowed to uniform.
8. application of the biological preparation described in claim 2 in turf grass drought resistance is improved.
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2016
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| CN106665571B (en) | 2019-10-01 |
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