CN106171360A - Euonymus carnosus cuttage breeding method - Google Patents
Euonymus carnosus cuttage breeding method Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
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- A—HUMAN NECESSITIES
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- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/10—Aromatic or araliphatic carboxylic acids, or thio analogues thereof; Derivatives thereof
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- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
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Abstract
Euonymus carnosus cuttage breeding method, belongs to technical field of plant propagation.Comprise the following steps: 1) choose suitable breeding spot and set up Euonymus carnosus female parent cutting orchard；2) substrate is installed with 72 hole dishes；3) suitable cutting time is early September or the middle ten days, cuttage in 1 year 1 time；4) cuttage branch uses and urges root agent to impregnate；5) after cuttage 40 days, the fringe bar root of hair of about 70% can start seedling exercising.Above-mentioned Euonymus carnosus cuttage breeding method, simple to operate, reproduction speed is fast, has captured the difficult problem that Euonymus carnosus cottage propagation survival rate is low, and yield rate is up to 90%, can be used for large-scale production.
Description
Technical field
The invention belongs to technical field of plant propagation, specially Euonymus carnosus cuttage breeding method.
Background technology
Euonymus carnosus Euonymus carnosus, Celastraceae, Euonymus, broad leaved and deciduous broad leaved dungarunga, height of tree 8m, the highest can
Reach 15m, diameter of a cross-section of a tree trunk 1.3 meters above the ground 10cm, bark Lycoperdon polymorphum Vitt.Branch cylindrical shape, celadon to taupe, sprout is yellow green.Dan Ye, to life, ground paper matter
To keratin, ellipse, oblong are to avette or long round shape obovate；Long 4-15cm, wide 1.5-7.0cm；Base portion wedge shape, tip
Blunt or short point, the little knuckle-tooth in edge or little sawtooth；Pinnate vein, lateral vein 8-12 pair, bend forward, at proximal edge again with veinlet
Interweave；Petiole 0.5-2.0cm；Leaf green, transfers shiny red or purple, more ornamental value to late autumn.Cyme is evacuated, and has
5-9, flower, spends green white, and petal is circular, and there are nest shape wrinkle or smooth, the month at florescence 5-8 in surface.Capsule, subsphaeroidal, yellowish-brown,
Brown is to bronzing, and 4 shallow split, seed every room 3-6, oval, dark brown, bright black, aril peony, really the phase 8-11 month.
Euonymus carnosus happiness light, the most resistance to shade, Salt And Alkali Tolerance；Being easier to by insect pest, Major Pests has control of Abraxas suspecta and Unaspis euonymi.
Originate in Southwest China, Himalaya intermountain, in Northeast China, each provinces and regions in North China and the middle and lower reach of Yangtze River, Liaoning, Hebei, Henan,
Shandong, Gansu, Anhui, Jiangsu, Zhejiang, Fujian, Jiangxi, Hubei, Sichuan etc. save all distribution.Chang Yesheng is in hillside shrubbery or woods
At edge.Euonymus carnosus belongs to the type that cuttage difficulty is taken root, and its nursery many employings seed propagation reported by existing document, but Euonymus carnosus
There is dormancy during seed maturity, it is impossible to directly sow, and germination percentage is low, is not suitable for large-scale breeding.Liu Feiyan etc. (1995) are to meat
Flower winged euonymus tissue culture has carried out preliminary study, but has no that scale is applied, and cost is high.Chen Liqing etc. (2004) use first
Cottage propagation Euonymus carnosus, found that cuttage survival rate is less than 8.6%.Euonymus carnosus cottage propagation survival rate is low is to restrict it
The bottleneck of large-scale production.Calendar year 2001, Senhe Seed Industry Co., Ltd., Zhejiang Pror. is female from mountain region, City of Taizhou wild state
This collection planting seed nursery, sets up seedling cutting orchard, carries out cottage propagation, through safety, regionality, compatibility test,
And use different plant to urge root agent, variable concentrations, different substrates formula and the test of cottage method, it is thus achieved that success.Meat flower is defended
The tree-like grace of lance, and the stronger salt resistance ability of tool, on island, light salt-soda soil, beach, mountain area all can be planted, and is excellent Landscape
See seeds and fabulous saline-alkali soil forestation seeds, it is possible to do potted landscape, hedgerow, isolated planting or mass-planting, be trained container seedling, high hedge Seedling and
Go alone Seedling, use isolated planting, avenue system or group planting, plant by garden, park, river, beach, be sight leaf that sight is good, see fruit and
Landscape plant, has wide market prospect.
Summary of the invention
For the above-mentioned problems in the prior art, it is an object of the invention to design provides a kind of Euonymus carnosus cuttage
The technical scheme of propagation method, comprises and sets up cutting orchard, and it is simple to operate, reproduction speed is fast, and yield rate is up to 90%.
Described Euonymus carnosus cuttage breeding method, it is characterised in that comprise the following steps:
1) choosing suitable breeding spot and set up Euonymus carnosus female parent cutting orchard, it is 50cm × 50cm that container seedling transplants seeding row spacing, close
Degree is for 1800-2000 plant/acre；
2) installing substrate with 72 hole dishes, keep substrate elasticity consistent, described substrate contains the following thing of weight percentage
Matter: peat 40-50%, furfural dregs 20-30%, yellow soil 8-15%, straw 10-20%, Radix Tripterygii Wilfordii ground product 3-5%；
3) suitable cutting time is early September or the middle ten days, and cuttage in 1 year 1 time cuts fringe from Euonymus carnosus female parent branch, and clip is selected in
The spring tip and the intersection of autumn growth last year, be cut into cuttage branch reservation 2 lateral buds, 2 opposite leaf sheets of a length of 4-5cm then,
And respectively cut off half, base portion flat nick；
4) cuttage branch uses and urges root agent 800-900ppm dipping fringe bar base portion 2-3 second, described in urge root agent be 2-by weight ratio
The streptomycin of 4:2-4:1-3:1-3: the basic element of cell division: ABT6 root-inducing powder: the mixing of Fericarpium Citri Limoniae total flavones extracting solution is constituted；
5) using 72 hole dishes to carry out cuttage, 1 fringe bar of every cave cuttage, the cuttage degree of depth is 2-3cm, waters permeable after cuttage immediately,
Spraying the carbendazim of 900-1000 times of liquid after the airing of blade face, move-in-mud robot, in canopy, air humidity reaches more than 95%, after cuttage
10 days, starting spray foliage fertilizer for the first time, later every 8-10 days, water and spray 1 time afterwards, foliage fertilizer can be with green fragrant Wei 900-
1000 times of liquid spray, and simultaneously with the spraying diseases prevention of 900-1000 times of liquid Mancozeb, after cuttage 40 days, the fringe bar of about 70% was sent out
Root can start seedling exercising.
Described Euonymus carnosus cuttage breeding method, it is characterised in that in step 1):
The selection soil texture is loose, fertile, cutting orchard is made in subacidity, neutral extremely subalkaline soil, turns over, ditches, makees bed,
Opening zanjon, seedbed bed surface width 120cm around field, bed surface soil block is whole carefully, and middle slightly higher, both sides are slightly lower；Pavement width 40cm, deeply
25cm, low centre, pavement two is slightly higher, forms certain gradient, it is ensured that draining is unimpeded；
Carrying out container seedling transplanting March in early November to next year, transplanting seeding row spacing is 50cm × 50cm, and density is 1700 plants/acre, fixed
Point digs cave, wipes out long side root, keeps the degree of depth consistent with the degree of depth of original plant, be compacted in root with fine earth heap during transplanting,
Irrigating in time and determine root water, bed surface one layer of Caulis et Folium Oryzae of paving, with moisturizing with prevent weed growth；
Seedling-slowing stage waters in time, such as continuous sunny, within the 4th day after transplanting, need to water, water once every 10 days later；In case of even
The continuous rainy day, timely draining；Full exposure is cultivated, and promotes fringe bar lignifying；Within after transplanting 15 days, starting fertilising, the 3-8 month is every half
The moon spreads fertilizer over the fields 1 carbamide or compound fertilizer, each every mu of 15kg, stops executing quick-acting fertilizer after JIUYUE, executes become thoroughly decomposed organic the 11-12 month 1 time
Fertilizer, every mu of 1500kg, buriing of ditching.When spreading fertilizer over the fields carbamide or compound fertilizer, carry out in conjunction with weeding with a hoe, execute in the ranks, with no seedling injury
Wood stem, branch and blade are as the criterion；After adopting fringe, execute 1 quick-acting fertilizer, every mu of 20kg immediately.
Described Euonymus carnosus cuttage breeding method, it is characterised in that step 2) described substrate contains weight percentage
Following material: peat 42-45%, furfural dregs 22-25%, yellow soil 10-12%, straw 12-15%, Radix Tripterygii Wilfordii ground product 4%.
Described Euonymus carnosus cuttage breeding method, it is characterised in that step 2) described in cutting medium use with lower section
Prepared by method:
1) pretreatment: be dried by Radix Tripterygii Wilfordii, pulverize, prepares Radix Tripterygii Wilfordii ground product；
2) by the following material mix homogeneously of weight percentage: peat 40-50%, furfural dregs 20-30%, yellow soil 8-15%, straw
Stalk 10-20%, Radix Tripterygii Wilfordii ground product 3-5%.
Described Euonymus carnosus cuttage breeding method, it is characterised in that step 2) described in Fericarpium Citri Limoniae total flavones extracting solution
Prepared by employing following methods:
1) Fericarpium Citri Limoniae drying and crushing is broken into coarse powder, cross 20 mesh sieves；
2) seepage pressure effects: Fericarpium Citri Limoniae coarse powder is loaded in percolator, after soaking 5 hours with 80% aquiferous ethanol, by 70% aqueous second
Alcohol carries out seepage pressure effects, if raw material weight is 1kg, the consumption of aquiferous ethanol is 5 liters, and flow velocity is 6mL/min, collects percolate；
3) flash concentration: by percolate bath temperature 80 DEG C, vacuum is 0.09 Mpa, and feed liquor flow velocity is 200mL min-1
Under conditions of carry out vacuum film concentration, reclaim ethanol, obtain every mL medicinal liquid and convert into the concentrated solution containing 2.0g raw material；
4) macroporous resin adsorption purification: by the concentrated solution that obtains by macroporous adsorbent resin separation and concentration, successively with water, 10% contain
Water-ethanol, 20% aquiferous ethanol, 30% aquiferous ethanol, 40% aquiferous ethanol, 50% aquiferous ethanol, 60% aquiferous ethanol carry out gradient and wash
De-, finally with 70% acetone eluting, collect each position eluent of 3 times of column volumes respectively, collect merging 20% aquiferous ethanol, 30%
Aquiferous ethanol, 40% aquiferous ethanol, the eluent at the 50% each position of aquiferous ethanol, the eluent of merging is Fericarpium Citri Limoniae total flavones and carries
Take liquid.
Described Euonymus carnosus cuttage breeding method, it is characterised in that in step 4): streptomycin: the basic element of cell division: ABT6
Number root-inducing powder: the weight ratio of Fericarpium Citri Limoniae total flavones extracting solution is 3:3:2:2.
Radix Tripterygii Wilfordii in above-mentioned substrate is the root of Celastraceae plant Radix Tripterygii Wilfordii, is again Caulis Fibraureae, cabbageworm medicine, water Folium illicii Lanceolati, main product
In Fujian, Zhejiang, Anhui, the ground such as Henan, in former plant is born in the shade how wet hillside, mountain valley, small stream limit bushes, has and drive
Dynamic, parasite killing, antiviral effect.Radix Tripterygii Wilfordii ground product in above-mentioned substrate, defending insect and killing is effective, and the raw material source of employing is in certainly
So, belong to botanical pesticide, slow release, long-acting insect expelling, parasite killing, and do not develop immunity to drugs, use safety.
Distiller grains in above-mentioned substrate are the side-products of brewing industry, containing crude protein 27.4%, crude fat 25%, crude fibre
10.5%, nitrogen-free extract 32.1%, cinder powder 5%.Distiller grains are added substrate, make up the defect that the yellow soil content of organic matter is not enough,
Improve crop yield.And hydrophobic substance is more, imbitition is little because of protein etc. for distiller grains, therefore aeration porosity is relatively big, for
45.5%；And coconut palm bran is because main component is the hydrophilic substances such as cellulose, produces imbitition, cause aeration porosity less, only
It is 4.7%.The addition of distiller grains, can improve the porosity physical behavior of substrate.
Furfural dregs in above-mentioned substrate is, after the agricultural byproducts such as corncob, bagasse are pulverized, a series of hydrolyzed chemical occurs
Remaining waste residue after furfural is extracted in reaction, and its rich cellulose, protein, moisture content and the porosity of furfural dregs are the highest, say
Its loose, porous bright, has good adsorptivity, improves the permeability of substrate.Open from nutritional labeling, the nitrogen of furfural dregs, phosphorus,
Potassium and organic content are higher, are conducive to improving the fertility state of substrate.
Straw in above-mentioned substrate is pulverized 2-4cm respectively by Semen arachidis hypogaeae bar, bean bar, and adjustment water content 55-70%, C/N compare 30-
32, it is piled into ridge, with covered rearing with plastic film, makes the abundant fermentation maturity of straw, respectively obtain become thoroughly decomposed Semen arachidis hypogaeae bar and the bean bar that becomes thoroughly decomposed, will
Becoming thoroughly decomposed Semen arachidis hypogaeae bar and the ratio of the bean bar 2:1 that becomes thoroughly decomposed is mixed to prepare, have establishing in large scale in China, once, resource is rich for annual results
Rich.
The cutting medium reasonable raw material proportioning that the present invention uses, environmentally safe, the nutrient of integrated substrate enriches, trace
Nutrient is complete, and cuttage shoot growth amount is had significant facilitation.
The above-mentioned basic element of cell division urged in root agent, calls 6-BA, 6-benzyl aminoadenine, is from Semen Maydis or other plant
A kind of N6-isopentennyladenine being separated to, at the material of the class promotion cytokinesis that plant root produces, promotes multiple group
The differentiation knitted and growth, have synergism, regulation plant cell growth and the phytohormone of growth with plant growing.
Urge addition Fericarpium Citri Limoniae total flavones extracting solution in root agent above-mentioned, the rooting rate of cuttage branch can be remarkably promoted, and
Improve its disease resistance.Containing substantial amounts of flavone compound in Fericarpium Citri Limoniae total flavones extracting solution, natural flavone compounds, do not contain
Any chemosynthesis class material, it can promote microorganism, plant cellular material operating and accumulation and cell enzyme activity, and then improve
The metabolic capacity of beneficial microbe bacterium, increases the disease resistance of plant, promotes plant growing.
In the present invention, cutting orchard is set up and management, cutting time, cuttings length, cutting medium form and quality, urge root agent
Concentration and formula is different, yield rate is all had a significant impact by management after cuttage.
Described plant ash, perlite, Maifanitum, straw, Celastrus angulatus ground product, Pericarpium Granati ground product, indolebutyric acid, thin
Born of the same parents' mitogen, Fericarpium Citri Limoniae total flavones extracting solution, green fragrant Wei, Mancozeb all can the most directly be buied.
Above-mentioned Euonymus carnosus cuttage breeding method, simple to operate, reproduction speed is fast, has captured Euonymus carnosus cottage propagation
The difficult problem that motility rate is low, yield rate is up to 90%, can be used for large-scale production.
The percentage composition related in present specification unless otherwise indicated, refers both to weight percentage.
Detailed description of the invention
In conjunction with embodiments of the invention, the invention will be further described.
1 cutting orchard is set up and management
1.1 ground, gardens
The selection soil texture is loose, fertile, cutting orchard is made in subacidity, neutral extremely subalkaline soil, turns over, ditches, makees bed,
Opening zanjon, seedbed bed surface width 120cm around field, bed surface soil block is whole carefully, and middle slightly higher, both sides are slightly lower；Pavement width 40cm, deeply
25cm, low centre, pavement two is slightly higher, forms certain gradient, it is ensured that draining is unimpeded.
1.2 transplant
March in early November to next year transplants.Graphic by seeding row spacing design configurations, transplanting seeding row spacing is 50cm × 50cm, close
Degree is 1700 plants/acre.Fixed point digs cave, wipes out long side root, keeps the degree of depth consistent with the degree of depth of original plant during transplanting, with thin
Mound is compacted in root, irrigates in time and determines root water, and bed surface one layer of Caulis et Folium Oryzae of paving, with moisturizing with prevent weed growth.
1.3 management
Seedling-slowing stage waters in time, such as continuous sunny, within the 4th day after transplanting, need to water, water once every 10 days later；In case of even
The continuous rainy day, timely draining；Full exposure is cultivated, and promotes fringe bar lignifying；Within after transplanting 15 days, starting fertilising, the 3-8 month is every half
The moon spreads fertilizer over the fields 1 carbamide or compound fertilizer, each every mu of 15kg, stops executing quick-acting fertilizer after JIUYUE, executes become thoroughly decomposed organic the 11-12 month 1 time
Fertilizer, every mu of 1500kg, buriing of ditching.When spreading fertilizer over the fields carbamide or compound fertilizer, carry out in conjunction with weeding with a hoe, execute in the ranks, with no seedling injury
Wood stem, branch and blade are as the criterion；After adopting fringe, execute 1 quick-acting fertilizer, every mu of 20kg immediately.Weeding to accomplish " except early, except little, remove
", substantially without weeds on seedbed；After rain, fine day weeds with a hoe in time, keeps soil from packing together.
2 breed
2.1 cut fringe
Early September or the middle ten days carry out cutting fringe, 1 year 1 time, and clip is selected in the spring tip and the intersection of autumn growth last year, cuttings length then
For 4cm, retain 2 lateral buds, 2 opposite leaf sheets, and respectively cut off half, base portion flat nick.Ground plants female parent then, cuts fringe high
Degree 15cm；Life in 2 years, life in 3 years and 4 years Rhizoma Anemarrhenae originally, cut fringe and retain maternal height respectively 25cm, 30cm and 35cm；To life in 5 years and
Below no longer as female parent, take stump measure in February, promote maternal base portion to sprout rejuvenation, improve fringe bar quality.
2.2 material
72 hole dish specifications are: wide 27cm, long 54cm, deep 5.5cm.Mesh bag specification is: diameter 6cm, deep 8cm.Described substrate contains
There is a following material of weight percentage: peat 40-50%, furfural dregs 20-30%, yellow soil 8-15%, straw 10-20%, Thunder God
Rattan ground product 3-5%.
2.3 cuttages and management
Using booth overhead seedbed cuttage, seedbed width 168cm, bed surface floor file cloth, laterally puts 3 hole tray or 3 pallets hold net
Bag；Bed surface, hole tray, mesh bag are in advance with 800 times of liquid disinfectants of potassium permanganate.
Using weight ratio is the streptomycin of 2-4:2-4:1-3:1-3: the basic element of cell division: ABT6 root-inducing powder: Fericarpium Citri Limoniae is total
Flavone extractive 1000ppm, quickly dipped fringe bar base portion 2 seconds, every cave or 1 fringe bar of each mesh bag cuttage, the cuttage degree of depth is 2cm；Skewer
Water permeable after Chaing immediately, after the airing of blade face, spray the carbendazim of 1000 times of liquid, then cover plastic sheeting, be inserted into bamboo chip little
Shed, sealing film surrounding, in making canopy, air humidity reaches more than 95%.
Set sunshade net at big ceiling, above seedbed, (at high 2m), set up two road sunshade nets, when Air Temperature in plastic sheeting
When degree is more than 38 DEG C, below two road sunshade nets, carry out mist cooling.Such as fine day high temperature, from point at 10 in the morning to afternoon 4,
Spray 1 time every half an hour.Every day checks soil humidity, can not overly moist, also can not overdrying, when the thin film reverse side full little water droplet of knot,
In Small plastic shed, relative air humidity is more than 95%, need not keep the skin wet；When little water droplet is little, water supply in media is not enough, be close to
Late or cloudy, open thin film, water permeable, immediately after by sealing in film cover, typically water 1 water every 7-10 days.
2.4 seedling exercising
After cuttage about 40 days, the fringe bar root of hair of about 70% can start seedling exercising.First open thin film two to ventilate 2 days, then exist
Dusk or cloudy day all open；Can manage with full exposure after 2 days at Small plastic shed upper cover last layer sunshade net after opening；
After cuttage 10 days, start spray foliage fertilizer for the first time, later every 7-10 days, water and spray 1 time afterwards.Foliage fertilizer can be with green fragrant Wei
1000 times of liquid, or 0.2% carbamide sprays or waters and execute.In conjunction with diseases prevention, with 1000 times of liquid Mancozeb sprayings.
3 commercial seedlings make
3.1 Plug seedling
By in tloig-cutlage to the hole tray in 72 holes, through 6 months take pains to foster, form Plug seedling.Seedling specification: the long 6cm of young sprout,
Well developed root system, robust plant, without pest and disease damage, yield rate 70%.
3.2 mesh bag Seedlings
By in tloig-cutlage to mesh bag, through 6 months take pains to foster, form mesh bag Seedling.Seedling specification: the long 6cm of young sprout, root system is sent out
Reaching, substrate does not dissipates, robust plant, without pest and disease damage, yield rate 70%.
3.3 container seedling
Plug seedling or mesh bag Seedling are moved in container, through 14 months take pains to foster, forms container seedling.Containers size: bore
12cm, degree of depth 10cm.Seedling specification: height of seedling 30cm, goes alone not bifurcated, yield rate 90%.
3.4 high hedge Seedlings
Color lump Seedling moved in 2 gallon container or is transplanted to big field, through three times of 18 months prunings with take pains to foster, forming height
Hedge Seedling.Containers size: bore 26cm, degree of depth 21cm.Seedling specification: height of seedling 120cm, be preced with width 60cm, one-level branch 2 (containing) with
On, one-level branching-point height 30cm；Second branch more than 4, second branch point height 60cm；Three grades of branches more than 8, three grades
Branching-point height 60cm, hat is well-balanced, yield rate 90%.
3.5 go alone Seedling
Height of seedling 30cm is gone alone transplantation of seedlings to big field, through 36 months take pains to foster, form the Seedling of going alone of height of seedling 180cm, become
Product rate 90%.
Claims (6)
1. Euonymus carnosus cuttage breeding method, it is characterised in that comprise the following steps:
1) choosing suitable breeding spot and set up Euonymus carnosus female parent cutting orchard, it is 50cm × 50cm that container seedling transplants seeding row spacing, close
Degree is for 1800-2000 plant/acre；
2) installing substrate with 72 hole dishes, keep substrate elasticity consistent, described substrate contains the following thing of weight percentage
Matter: peat 40-50%, furfural dregs 20-30%, yellow soil 8-15%, straw 10-20%, Radix Tripterygii Wilfordii ground product 3-5%；
3) suitable cutting time is early September or the middle ten days, and cuttage in 1 year 1 time cuts fringe from Euonymus carnosus female parent branch, and clip is selected in
The spring tip and the intersection of autumn growth last year, be cut into cuttage branch reservation 2 lateral buds, 2 opposite leaf sheets of a length of 4-5cm then,
And respectively cut off half, base portion flat nick；
4) cuttage branch uses and urges root agent 800-900ppm dipping fringe bar base portion 2-3 second, described in urge root agent be 2-by weight ratio
The streptomycin of 4:2-4:1-3:1-3: the basic element of cell division: ABT6 root-inducing powder: the mixing of Fericarpium Citri Limoniae total flavones extracting solution is constituted；
5) using 72 hole dishes to carry out cuttage, 1 fringe bar of every cave cuttage, the cuttage degree of depth is 2-3cm, waters permeable after cuttage immediately,
Spraying the carbendazim of 900-1000 times of liquid after the airing of blade face, move-in-mud robot, in canopy, air humidity reaches more than 95%, after cuttage
10 days, starting spray foliage fertilizer for the first time, later every 8-10 days, water and spray 1 time afterwards, foliage fertilizer can be with green fragrant Wei 900-
1000 times of liquid spray, and simultaneously with the spraying diseases prevention of 900-1000 times of liquid Mancozeb, after cuttage 40 days, the fringe bar of about 70% was sent out
Root can start seedling exercising.
2. Euonymus carnosus cuttage breeding method as claimed in claim 1, it is characterised in that in step 1):
The selection soil texture is loose, fertile, cutting orchard is made in subacidity, neutral extremely subalkaline soil, turns over, ditches, makees bed,
Opening zanjon, seedbed bed surface width 120cm around field, bed surface soil block is whole carefully, and middle slightly higher, both sides are slightly lower；Pavement width 40cm, deeply
25cm, low centre, pavement two is slightly higher, forms certain gradient, it is ensured that draining is unimpeded；
Carrying out container seedling transplanting March in early November to next year, transplanting seeding row spacing is 50cm × 50cm, and density is 1700 plants/acre, fixed
Point digs cave, wipes out long side root, keeps the degree of depth consistent with the degree of depth of original plant, be compacted in root with fine earth heap during transplanting,
Irrigating in time and determine root water, bed surface one layer of Caulis et Folium Oryzae of paving, with moisturizing with prevent weed growth；
Seedling-slowing stage waters in time, such as continuous sunny, within the 4th day after transplanting, need to water, water once every 10 days later；In case of even
The continuous rainy day, timely draining；Full exposure is cultivated, and promotes fringe bar lignifying；Within after transplanting 15 days, starting fertilising, the 3-8 month is every half
The moon spreads fertilizer over the fields 1 carbamide or compound fertilizer, each every mu of 15kg, stops executing quick-acting fertilizer after JIUYUE, executes become thoroughly decomposed organic the 11-12 month 1 time
Fertilizer, every mu of 1500kg, buriing of ditching；
When spreading fertilizer over the fields carbamide or compound fertilizer, carry out in conjunction with weeding with a hoe, execute in the ranks, be as the criterion with no seedling injury wood stem, branch and blade；
After adopting fringe, execute 1 quick-acting fertilizer, every mu of 20kg immediately.
3. Euonymus carnosus cuttage breeding method as claimed in claim 1, it is characterised in that step 2) described substrate contains weight
The following material of percentage composition: peat 42-45%, furfural dregs 22-25%, yellow soil 10-12%, straw 12-15%, Radix Tripterygii Wilfordii are pulverized
Thing 4%.
4. Euonymus carnosus cuttage breeding method as claimed in claim 1, it is characterised in that step 2) described in cutting medium
Prepared by employing following methods:
1) pretreatment: be dried by Radix Tripterygii Wilfordii, pulverize, prepares Radix Tripterygii Wilfordii ground product；
2) by the following material mix homogeneously of weight percentage: peat 40-50%, furfural dregs 20-30%, yellow soil 8-15%, straw
Stalk 10-20%, Radix Tripterygii Wilfordii ground product 3-5%.
5. Euonymus carnosus cuttage breeding method as claimed in claim 1, it is characterised in that step 2) described in Fericarpium Citri Limoniae total
Flavone extractive uses following methods to prepare:
1) Fericarpium Citri Limoniae drying and crushing is broken into coarse powder, cross 20 mesh sieves；
2) seepage pressure effects: Fericarpium Citri Limoniae coarse powder is loaded in percolator, after soaking 5 hours with 80% aquiferous ethanol, by 70% aqueous second
Alcohol carries out seepage pressure effects, if raw material weight is 1kg, the consumption of aquiferous ethanol is 5 liters, and flow velocity is 6mL/min, collects percolate；
3) flash concentration: by percolate bath temperature 80 DEG C, vacuum is 0.09 Mpa, and feed liquor flow velocity is 200mL min-1
Under conditions of carry out vacuum film concentration, reclaim ethanol, obtain every mL medicinal liquid and convert into the concentrated solution containing 2.0g raw material；
4) macroporous resin adsorption purification: by the concentrated solution that obtains by macroporous adsorbent resin separation and concentration, successively with water, 10% contain
Water-ethanol, 20% aquiferous ethanol, 30% aquiferous ethanol, 40% aquiferous ethanol, 50% aquiferous ethanol, 60% aquiferous ethanol carry out gradient and wash
De-, finally with 70% acetone eluting, collect each position eluent of 3 times of column volumes respectively, collect merging 20% aquiferous ethanol, 30%
Aquiferous ethanol, 40% aquiferous ethanol, the eluent at the 50% each position of aquiferous ethanol, the eluent of merging is Fericarpium Citri Limoniae total flavones and carries
Take liquid.
6. Euonymus carnosus cuttage breeding method as claimed in claim 1, it is characterised in that in step 4): streptomycin: cell divides
Split element: ABT6 root-inducing powder: the weight ratio of Fericarpium Citri Limoniae total flavones extracting solution is 3:3:2:2.
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| CN113141888A (en) * | 2020-01-07 | 2021-07-23 | 云南罗瑞生物科技有限公司 | Quick cutting propagation and seedling raising technology for medicinal vine tripterygium wilfordii twigs |
| CN116616065A (en) * | 2023-06-20 | 2023-08-22 | 东北农业大学 | Summer cutting method for verbena |
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| CN102498863A (en) * | 2011-10-31 | 2012-06-20 | 浙江森禾种业股份有限公司 | Microbiota decussata cutting propagation method |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113141888A (en) * | 2020-01-07 | 2021-07-23 | 云南罗瑞生物科技有限公司 | Quick cutting propagation and seedling raising technology for medicinal vine tripterygium wilfordii twigs |
| CN116616065A (en) * | 2023-06-20 | 2023-08-22 | 东北农业大学 | Summer cutting method for verbena |
| CN116616065B (en) * | 2023-06-20 | 2024-04-26 | 东北农业大学 | Summer cutting method for verbena |
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