CN105776573A - Method for processing emulsified oil in soybean oil waste water - Google Patents
Method for processing emulsified oil in soybean oil waste water Download PDFInfo
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Abstract
The invention aims to provide a method for processing emulsified oil in soybean oil waste water, and belongs to the technical field of microbial technology and environmental engineering. According to the method provided by the invention, acinetobacter calcoaceticus is used for performing emulsion-breaking treatment on the emulsified oil in the soybean oil waste water; then acinetobacter johnsonii is used for producing a microbial flocculant for performing flocculation treatment on the emulsion-broken emulsified oil to remove the emulsified oil in the soybean oil waste water. The method comprises the following steps: (1) preparing a bacterial suspension of acinetobacter calcoaceticus; (2) preparing the microbial flocculant with acinetobacter johnsonii; (3) performing emulsion-breaking treatment on the emulsified oil in the soybean oil waste water; (4) processing the emulsion-broken emulsified oil. The method provided by the invention has a high emulsified oil emulsion-breaking rate kept at 95.39%-96.70%; the microbial flocculant produced by the acinetobacter johnsonii is used for flocculation removal of the emulsion-broken emulsified oil, so as to achieve a higher removal rate of the emulsified oil in the soybean oil waste water, kept at 92.01%-94.45%, and also achieve a higher removal rate of COD in the soybean oil waste water, kept at 47.32%-50.88%.
Description
Technical field
The invention belongs to microbial technique and field of environment engineering technology.
Background technology
Soybean oil waste water mainly includes the waste water of the course of processing discharges such as the condenser draining in the alkali refining waste water of refining plant, centrifuge flushing water, leaching workshop.This kind of waste water is characterized by that oils and fats and content of organics are high, as without processing directly discharge, caused serious water environment pollution.
Domestic soybean oil waste water processes the technique mainly taked and includes oil removal process, coagulation or air-flotation process, biological treatment etc..Wherein biological treatment is the core process that soybean oil waste water processes, and Main Function is to remove the Organic substance in waste water；Oil removal process, coagulation or air-flotation process belong to pretreating process, and Main Function is to remove the oils and fats in waste water, creates a good running environment for biological treatment.Oil removal processes the main oil slick removed in soybean oil waste water and dispersed oil, and oil emulsion elaioleucite diameter is little, stable in properties, it is difficult to it is processed by common air supporting or coagulation, and then affecting the properly functioning of biological treatment system, the oil emulsion therefore removed in soybean oil waste water is the difficult point in soybean oil waste water preconditioning technique and emphasis.
Breakdown of emulsion is particularly significant in the processing procedure of oil emulsion, and oil emulsion, once process through breakdown of emulsion, is removed being easy in follow-up coagulation or air-flotation process process.Existing demulsification technology mainly adds chemical demulsifier, but it has, and selectivity is strong, dosage big, the shortcoming of difficult for biological degradation, and environment often causes in use procedure serious pollution.Biological demulsifying agent is the biological preparation that microbial metabolism produced, can make oil-water separation, including the microbial metabolism extracellular products with amphipathic characteristic produced and the biological demulsifying bacteria with special surface character and material.Wherein, structure, abundant functional group and the material that biological demulsifying bacteria surface is complicated makes it have highly efficient demulsification performance and better adaptability, it is possible to the oil emulsion in soybean oil waste water is carried out breakdown of emulsion process.
Summary of the invention
It is an object of the invention to utilize acinetobacter calcoaceticus that the oil emulsion in soybean oil waste water is carried out breakdown of emulsion process；Then utilize Acinetobacter johnsonii to produce microbial flocculant, the oil emulsion after breakdown of emulsion is carried out flocculation treatment, remove the method that soybean oil waste water oil emulsion is processed of soybean oil waste water oil emulsion.
The step of the present invention is:
(1) preparation of acinetobacter calcoaceticus bacteria suspension:
Being linked in the aseptic seed culture medium of 100mL by a strain list bacterium colony of the acinetobacter calcoaceticus that the strain number being saved on inclined-plane is CGMCCNO:1.2906, wherein in acinetobacter calcoaceticus seed culture medium, each amounts of components is respectively as follows: yeast extract powder 3g/L；Peptone 8g/L；NaCl6g/L；pH=7.5；30 DEG C of shaking tables are cultivated 1 day with 180rpm, obtains cultured acinetobacter calcoaceticus bacteria suspension, the bacteria suspension obtained is left in 4 DEG C of refrigerators and save backup；
(2) Acinetobacter johnsonii prepares microbial flocculant:
Being linked in the aseptic fermentation medium of 100mL by a strain list bacterium colony of the Acinetobacter johnsonii that the strain number being saved on inclined-plane is CGMCCNO:1.8030, in described Acinetobacter johnsonii fermentation medium, each amounts of components is respectively as follows: glucose 15.0g/L；K2HPO43g/L；KH2PO43g/L；Carbamide 0.3g/L；Yeast extract 0.3g/L；MgSO4·7H2O0.1g/L；NaCl0.1g/L；pH=7.5；30 DEG C of shaking tables are cultivated 5 days with 150rpm, obtains cultured Acinetobacter johnsonii microbial flocculant；After being cooled to room temperature, microbial flocculant is left in 4 DEG C of refrigerators and save backup；
(3) oil emulsion in soybean oil waste water is carried out breakdown of emulsion:
Take the acinetobacter calcoaceticus bacteria suspension that 10～20mL step (1) obtains and put into the soybean oil waste water after 200mL oil removal processes, the pH=7.5 of the soybean oil waste water after oil removal processes is regulated with the NaOH that concentration is 0.01mol/L, 10min is quickly stirred with 300rpm with coagulation agitator, then stand 1 day in 32 DEG C of incubators, obtain the soybean oil waste water of oil emulsion breakdown of emulsion, measure the demulsification efficiency of soybean oil waste water oil emulsion；
(4) oil emulsion after breakdown of emulsion processes:
Take the microbial flocculant that 10～20mL step (2) obtains, put in the soybean oil waste water of the oil emulsion breakdown of emulsion that 200mL step (3) obtains, the pH=7.5 of the soybean oil waste water of breakdown of emulsion is regulated with the NaOH that concentration is 0.01mol/L, 2min is quickly stirred with 300rpm with coagulation agitator, then with 120rpm low rate mixing 10min, after standing 20 minutes, measure the clearance of soybean oil waste water oil emulsion and the clearance of COD.
The positive effect of the present invention:
1, utilizing acinetobacter calcoaceticus to after oil emulsion breakdown of emulsion in soybean oil waste water, the oil emulsion after breakdown of emulsion is carried out flocculation and removes by the microbial flocculant that recycling Acinetobacter johnsonii produces, and processing procedure energy consumption is low, and environment does not produce secondary pollution；
2, utilizing acinetobacter calcoaceticus that oil emulsion in soybean oil waste water is carried out breakdown of emulsion, oil emulsion demulsification efficiency is higher, is maintained at 95.39%～96.70%；Oil emulsion after breakdown of emulsion is carried out flocculation and removes by the microbial flocculant that recycling Acinetobacter johnsonii produces, and soybean oil waste water oil emulsion clearance and COD clearance are all higher, are kept at 92.01%～94.45% and 47.32%～50.88%.
Detailed description of the invention
The step of the present invention is:
(1) preparation of acinetobacter calcoaceticus bacteria suspension:
Being linked in the aseptic seed culture medium of 100mL by a strain list bacterium colony of the acinetobacter calcoaceticus (Acinetobactercalcoaceticus) that the strain number being saved on inclined-plane is CGMCCNO:1.2906, wherein in acinetobacter calcoaceticus seed culture medium, each amounts of components is respectively as follows: yeast extract powder 3g/L；Peptone 8g/L；NaCl6g/L；pH=7.5；30 DEG C of shaking tables are cultivated 1 day with 180rpm, obtains cultured acinetobacter calcoaceticus bacteria suspension, the bacteria suspension obtained is left in 4 DEG C of refrigerators and save backup；
(2) Acinetobacter johnsonii prepares microbial flocculant:
Being linked in the aseptic fermentation medium of 100mL by a strain list bacterium colony of the Acinetobacter johnsonii (Acinetobacterjohnsonii) that the strain number being saved on inclined-plane is CGMCCNO:1.8030, in described Acinetobacter johnsonii fermentation medium, each amounts of components is respectively as follows: glucose 15.0g/L；K2HPO43g/L；KH2PO43g/L；Carbamide 0.3g/L；Yeast extract 0.3g/L；MgSO4·7H2O0.1g/L；NaCl0.1g/L；pH=7.5；30 DEG C of shaking tables are cultivated 5 days with 150rpm, obtains cultured Acinetobacter johnsonii microbial flocculant；After being cooled to room temperature, microbial flocculant is left in 4 DEG C of refrigerators and save backup；
(3) strain number is that the oil emulsion in soybean oil waste water is carried out breakdown of emulsion by the acinetobacter calcoaceticus of CGMCCNO:1.2906:
Take the acinetobacter calcoaceticus bacteria suspension that 10～20mL step (1) obtains and put into the soybean oil waste water after 200mL oil removal processes, the pH=7.5 of the soybean oil waste water after oil removal processes is regulated with the NaOH that concentration is 0.01mol/L, 10min is quickly stirred with 300rpm with coagulation agitator, then stand 1 day in 32 DEG C of incubators, obtain the soybean oil waste water of oil emulsion breakdown of emulsion, measure the demulsification efficiency of soybean oil waste water oil emulsion；
(4) strain number is that the oil emulsion after breakdown of emulsion in soybean oil waste water is processed by the Acinetobacter johnsonii of CGMCCNO:1.8030:
Take the microbial flocculant that 10～20mL step (2) obtains, put in the soybean oil waste water of the oil emulsion breakdown of emulsion that 200mL step (3) obtains, the pH=7.5 of the soybean oil waste water of breakdown of emulsion is regulated with the NaOH that concentration is 0.01mol/L, 2min is quickly stirred with 300rpm with coagulation agitator, then with 120rpm low rate mixing 10min, after standing 20 minutes, measure the clearance of soybean oil waste water oil emulsion and the clearance of COD.Realize the removal of soybean oil waste water oil emulsion, provide good environment for subsequent biological treatment technique.
Below the present invention is described in further details:
The present invention utilizes acinetobacter calcoaceticus that the oil emulsion in soybean oil waste water is carried out breakdown of emulsion process；Then utilize Acinetobacter johnsonii to produce microbial flocculant, the oil emulsion after breakdown of emulsion is carried out flocculation treatment, remove the oil emulsion of soybean oil waste water, provide good environment to soybean oil waste water subsequent biological treatment technique.
Oil emulsion after breakdown of emulsion is had good removal effect by existing air-float technology, but processing procedure needs to expend a large amount of electric energy.Oil emulsion after breakdown of emulsion is had good removal effect by flocculation process equally.Biological flocculant has the 26S Proteasome Structure and Function of uniqueness, and the oil emulsion after breakdown of emulsion has good flocculating sedimentation removal effect, and environment is not produced to pollute by processing procedure.
Embodiment 1(utilizes merely the microbial flocculant that Acinetobacter johnsonii produces that the oil emulsion in soybean oil waste water carries out flocculation and removes):
(1) Acinetobacter johnsonii prepares microbial flocculant
Being linked in the aseptic fermentation medium of 100mL by a strain list bacterium colony of the Acinetobacter johnsonii (Acinetobacterjohnsonii) that the strain number being saved on inclined-plane is CGMCCNO:1.8030, in described Acinetobacter johnsonii fermentation medium, each amounts of components is respectively as follows: glucose 15.0g/L；K2HPO43g/L；KH2PO43g/L；Carbamide 0.3g/L；Yeast extract 0.3g/L；MgSO4·7H2O0.1g/L；NaCl0.1g/L；pH=7.5；30 DEG C of shaking tables are cultivated 5 days with 150rpm, obtains cultured Acinetobacter johnsonii microbial flocculant；After being cooled to room temperature, microbial flocculant is left in 4 DEG C of refrigerators and save backup；
(2) strain number is that the oil emulsion after breakdown of emulsion in soybean oil waste water is processed by the Acinetobacter johnsonii of CGMCCNO:1.8030
Take the microbial flocculant that 15mL step (1) obtains, put into the soybean oil waste water after 200mL oil removal processes, the pH=7.5 of the soybean oil waste water of breakdown of emulsion is regulated with the NaOH that concentration is 0.01mol/L, 2min is quickly stirred with 300rpm with coagulation agitator, then with 120rpm low rate mixing 10min, after standing 20 minutes, measure the clearance of soybean oil waste water oil emulsion and the clearance of COD.The removal effect of soybean oil waste water oil emulsion is in Table 1.
The table 115mL Acinetobacter johnsonii microbial flocculant removal effect to 200mL soybean oil waste water oil emulsion
As can be seen from Table 1, merely through the process of microbial flocculant, in soybean oil waste water, oil emulsion clearance is very low.
Embodiment 2(utilizes acinetobacter calcoaceticus to after oil emulsion breakdown of emulsion in soybean oil waste water, and the oil emulsion after breakdown of emulsion is removed by recycling inorganic flocculating agent polyaluminium sulfate)
(1) preparation of acinetobacter calcoaceticus bacteria suspension
Being linked in the aseptic seed culture medium of 100mL by a strain list bacterium colony of the acinetobacter calcoaceticus (Acinetobactercalcoaceticus) that the strain number being saved on inclined-plane is CGMCCNO:1.2906, in described acinetobacter calcoaceticus seed culture medium, each amounts of components is respectively as follows: yeast extract powder 3g/L；Peptone 8g/L；NaCl6g/L；pH=7.5；30 DEG C of shaking tables are cultivated 1 day with 180rpm, obtains cultured acinetobacter calcoaceticus bacteria suspension, the bacteria suspension obtained is left in 4 DEG C of refrigerators and save backup；
(2) strain number is that the oil emulsion in soybean oil waste water is carried out breakdown of emulsion by the acinetobacter calcoaceticus of CGMCCNO:1.2906
Take the acinetobacter calcoaceticus bacteria suspension that 15mL step (1) obtains and put into the soybean oil waste water after 200mL oil removal processes, the pH=7.5 of the soybean oil waste water after oil removal processes is regulated with the NaOH that concentration is 0.01mol/L, 10min is quickly stirred with 300rpm with coagulation agitator, then stand 1 day in 32 DEG C of incubators, obtain the soybean oil waste water of oil emulsion breakdown of emulsion, measure the demulsification efficiency of soybean oil waste water oil emulsion；
(3) oil emulsion after breakdown of emulsion in soybean oil waste water is processed by polyaluminium sulfate
Take 50mLAl2(SO4)3·18H2O puts into the soybean oil waste water of the oil emulsion breakdown of emulsion that 200mL step (2) obtains, described Al2(SO4)3·18H2O concentration is 0.35g/L, the pH=7.5 of the soybean oil waste water of breakdown of emulsion is regulated with the NaOH that concentration is 0.01mol/L, 2min is quickly stirred with 300rpm with coagulation agitator, then with 120rpm low rate mixing 10min, after standing 20 minutes, measure the clearance of soybean oil waste water oil emulsion and the clearance of COD.The removal effect of soybean oil waste water oil emulsion is in Table 2.
Table 215mL acinetobacter calcoaceticus bacteria suspension and the 50mL polyaluminium sulfate removal effect to 200mL soybean oil waste water oil emulsion
As can be seen from Table 2, can be removed through the process of acinetobacter calcoaceticus and polyaluminium sulfate, soybean oil waste water oil emulsion and COD, but removal effect is undesirable, and easily caused by aluminum ions pollution in processing procedure.
Embodiment 3(utilizes acinetobacter calcoaceticus to after oil emulsion breakdown of emulsion in soybean oil waste water, and the oil emulsion after breakdown of emulsion is removed by recycling Acinetobacter johnsonii microbial flocculant)
(1) preparation of acinetobacter calcoaceticus bacteria suspension
Being linked in the aseptic seed culture medium of 100mL by a strain list bacterium colony of the acinetobacter calcoaceticus (Acinetobactercalcoaceticus) that the strain number being saved on inclined-plane is CGMCCNO:1.2906, in described acinetobacter calcoaceticus seed culture medium, each amounts of components is respectively as follows: yeast extract powder 3g/L；Peptone 8g/L；NaCl6g/L；pH=7.5；30 DEG C of shaking tables are cultivated 1 day with 180rpm, obtains cultured acinetobacter calcoaceticus bacteria suspension, the bacteria suspension obtained is left in 4 DEG C of refrigerators and save backup；
(2) Acinetobacter johnsonii prepares microbial flocculant
Being linked in the aseptic fermentation medium of 100mL by a strain list bacterium colony of the Acinetobacter johnsonii (Acinetobacterjohnsonii) that the strain number being saved on inclined-plane is CGMCCNO:1.8030, in described Acinetobacter johnsonii fermentation medium, each amounts of components is respectively as follows: glucose 15.0g/L；K2HPO43g/L；KH2PO43g/L；Carbamide 0.3g/L；Yeast extract 0.3g/L；MgSO4·7H2O0.1g/L；NaCl0.1g/L；pH=7.5；30 DEG C of shaking tables are cultivated 5 days with 150rpm, obtains cultured Acinetobacter johnsonii microbial flocculant；After being cooled to room temperature, microbial flocculant is left in 4 DEG C of refrigerators and save backup；
(3) strain number is that the oil emulsion in soybean oil waste water is carried out breakdown of emulsion by the acinetobacter calcoaceticus of CGMCCNO:1.2906
Take the acinetobacter calcoaceticus bacteria suspension that 10mL step (1) obtains and put into the soybean oil waste water after 200mL oil removal processes, the pH=7.5 of the soybean oil waste water after oil removal processes is regulated with the NaOH that concentration is 0.01mol/L, 10min is quickly stirred with 300rpm with coagulation agitator, then stand 1 day in 32 DEG C of incubators, obtain the soybean oil waste water of oil emulsion breakdown of emulsion, measure the demulsification efficiency of soybean oil waste water oil emulsion；
(4) strain number is that the oil emulsion after breakdown of emulsion in soybean oil waste water is processed by the Acinetobacter johnsonii of CGMCCNO:1.8030
Take the microbial flocculant that 10mL step (2) obtains, put in the soybean oil waste water of the oil emulsion breakdown of emulsion that 200mL step (3) obtains, the pH=7.5 of the soybean oil waste water of breakdown of emulsion is regulated with the NaOH that concentration is 0.01mol/L, 2min is quickly stirred with 300rpm with coagulation agitator, then with 120rpm low rate mixing 10min, after standing 20 minutes, measure the clearance of soybean oil waste water oil emulsion and the clearance of COD, realize the removal of soybean oil waste water oil emulsion, provide good environment for subsequent biological treatment technique.The demulsification efficiency of soybean oil waste water oil emulsion and the removal effect of oil emulsion are in Table 3.
Table 310mL acinetobacter calcoaceticus bacteria suspension and the 10mL Acinetobacter johnsonii microbial flocculant removal effect to 200mL soybean oil waste water oil emulsion
As can be seen from Table 3, through the process of acinetobacter calcoaceticus and Acinetobacter johnsonii, soybean oil waste water oil emulsion and COD have good removal effect.
Embodiment 4(utilizes acinetobacter calcoaceticus to after oil emulsion breakdown of emulsion in soybean oil waste water, and the oil emulsion after breakdown of emulsion is removed by recycling inorganic flocculating agent polyaluminium sulfate, its (1st) and (2nd) step identical with embodiment 3, omission herein)
(3) strain number is that the oil emulsion in soybean oil waste water is carried out breakdown of emulsion by the acinetobacter calcoaceticus of CGMCCNO:1.2906
Take the acinetobacter calcoaceticus bacteria suspension that 10mL step (1) obtains and put into the soybean oil waste water after 200mL oil removal processes, the pH=7.5 of the soybean oil waste water after oil removal processes is regulated with the NaOH that concentration is 0.01mol/L, 10min is quickly stirred with 300rpm with coagulation agitator, then stand 1 day in 32 DEG C of incubators, obtain the soybean oil waste water of oil emulsion breakdown of emulsion, measure the demulsification efficiency of soybean oil waste water oil emulsion；
(4) strain number is that the oil emulsion after breakdown of emulsion in soybean oil waste water is processed by the Acinetobacter johnsonii of CGMCCNO:1.8030
Take the microbial flocculant that 20mL step (2) obtains, put in the soybean oil waste water of the oil emulsion breakdown of emulsion that 200mL step (3) obtains, the pH=7.5 of the soybean oil waste water of breakdown of emulsion is regulated with the NaOH that concentration is 0.01mol/L, 2min is quickly stirred with 300rpm with coagulation agitator, then with 120rpm low rate mixing 10min, after standing 20 minutes, measure the clearance of soybean oil waste water oil emulsion and the clearance of COD, realize the removal of soybean oil waste water oil emulsion, provide good environment for subsequent biological treatment technique.The demulsification efficiency of soybean oil waste water oil emulsion and the removal effect of oil emulsion are in Table 4.
Table 410mL acinetobacter calcoaceticus bacteria suspension and the 20mL Acinetobacter johnsonii microbial flocculant removal effect to 200mL soybean oil waste water oil emulsion
As can be seen from Table 4, through the process of acinetobacter calcoaceticus and Acinetobacter johnsonii, soybean oil waste water oil emulsion and COD have good removal effect.
Embodiment 5(utilizes acinetobacter calcoaceticus to after oil emulsion breakdown of emulsion in soybean oil waste water, and the oil emulsion after breakdown of emulsion is removed by recycling inorganic flocculating agent polyaluminium sulfate, its (1st) and (2nd) step identical with embodiment 3, omission herein)
(3) strain number is that the oil emulsion in soybean oil waste water is carried out breakdown of emulsion by the acinetobacter calcoaceticus of CGMCCNO:1.2906
Take the acinetobacter calcoaceticus bacteria suspension that 15mL step (1) obtains and put into the soybean oil waste water after 200mL oil removal processes, the pH=7.5 of the soybean oil waste water after oil removal processes is regulated with the NaOH that concentration is 0.01mol/L, 10min is quickly stirred with 300rpm with coagulation agitator, then stand 1 day in 32 DEG C of incubators, obtain the soybean oil waste water of oil emulsion breakdown of emulsion, measure the demulsification efficiency of soybean oil waste water oil emulsion；
(4) strain number is that the oil emulsion after breakdown of emulsion in soybean oil waste water is processed by the Acinetobacter johnsonii of CGMCCNO:1.8030
Take the microbial flocculant that 15mL step (2) obtains, put in the soybean oil waste water of the oil emulsion breakdown of emulsion that 200mL step (3) obtains, the pH=7.5 of the soybean oil waste water of breakdown of emulsion is regulated with the NaOH that concentration is 0.01mol/L, 2min is quickly stirred with 300rpm with coagulation agitator, then with 120rpm low rate mixing 10min, after standing 20 minutes, measure the clearance of soybean oil waste water oil emulsion and the clearance of COD, realize the removal of soybean oil waste water oil emulsion, provide good environment for subsequent biological treatment technique.The demulsification efficiency of soybean oil waste water oil emulsion and the removal effect of oil emulsion are in Table 5.
Table 515mL acinetobacter calcoaceticus bacteria suspension and the 15mL Acinetobacter johnsonii microbial flocculant removal effect to 200mL soybean oil waste water oil emulsion
As can be seen from Table 5, through the process of acinetobacter calcoaceticus and Acinetobacter johnsonii, soybean oil waste water oil emulsion and COD have good removal effect.
Embodiment 6(utilizes acinetobacter calcoaceticus to after oil emulsion breakdown of emulsion in soybean oil waste water, and the oil emulsion after breakdown of emulsion is removed by recycling inorganic flocculating agent polyaluminium sulfate, its (1st) and (2nd) step identical with embodiment 3, omission herein)
(3) strain number is that the oil emulsion in soybean oil waste water is carried out breakdown of emulsion by the acinetobacter calcoaceticus of CGMCCNO:1.2906
Take the acinetobacter calcoaceticus bacteria suspension that 20mL step (1) obtains and put into the soybean oil waste water after 200mL oil removal processes, the pH=7.5 of the soybean oil waste water after oil removal processes is regulated with the NaOH that concentration is 0.01mol/L, 10min is quickly stirred with 300rpm with coagulation agitator, then stand 1 day in 32 DEG C of incubators, obtain the soybean oil waste water of oil emulsion breakdown of emulsion, measure the demulsification efficiency of soybean oil waste water oil emulsion；
(4) strain number is that the oil emulsion after breakdown of emulsion in soybean oil waste water is processed by the Acinetobacter johnsonii of CGMCCNO:1.8030
Take the microbial flocculant that 20mL step (2) obtains, put in the soybean oil waste water of the oil emulsion breakdown of emulsion that 200mL step (3) obtains, the pH=7.5 of the soybean oil waste water of breakdown of emulsion is regulated with the NaOH that concentration is 0.01mol/L, 2min is quickly stirred with 300rpm with coagulation agitator, then with 120rpm low rate mixing 10min, after standing 20 minutes, measure the clearance of soybean oil waste water oil emulsion and the clearance of COD, realize the removal of soybean oil waste water oil emulsion, provide good environment for subsequent biological treatment technique.The demulsification efficiency of soybean oil waste water oil emulsion and the removal effect of oil emulsion are in Table 6.
Table 620mL acinetobacter calcoaceticus bacteria suspension and the 20mL Acinetobacter johnsonii microbial flocculant removal effect to 200mL soybean oil waste water oil emulsion
As can be seen from Table 6, through the process of acinetobacter calcoaceticus and Acinetobacter johnsonii, soybean oil waste water oil emulsion and COD have good removal effect.
Claims (1)
1. the method that soybean oil waste water oil emulsion is processed, it is characterised in that: the steps include:
(1) preparation of acinetobacter calcoaceticus bacteria suspension:
Being linked in the aseptic seed culture medium of 100mL by a strain list bacterium colony of the acinetobacter calcoaceticus that the strain number being saved on inclined-plane is CGMCCNO:1.2906, wherein in acinetobacter calcoaceticus seed culture medium, each amounts of components is respectively as follows: yeast extract powder 3g/L；Peptone 8g/L；NaCl6g/L；pH=7.5；30 DEG C of shaking tables are cultivated 1 day with 180rpm, obtains cultured acinetobacter calcoaceticus bacteria suspension, the bacteria suspension obtained is left in 4 DEG C of refrigerators and save backup；
(2) Acinetobacter johnsonii prepares microbial flocculant:
Being linked in the aseptic fermentation medium of 100mL by a strain list bacterium colony of the Acinetobacter johnsonii that the strain number being saved on inclined-plane is CGMCCNO:1.8030, in described Acinetobacter johnsonii fermentation medium, each amounts of components is respectively as follows: glucose 15.0g/L；K2HPO43g/L；KH2PO43g/L；Carbamide 0.3g/L；Yeast extract 0.3g/L；MgSO4·7H2O0.1g/L；NaCl0.1g/L；pH=7.5；30 DEG C of shaking tables are cultivated 5 days with 150rpm, obtains cultured Acinetobacter johnsonii microbial flocculant；After being cooled to room temperature, microbial flocculant is left in 4 DEG C of refrigerators and save backup；
(3) oil emulsion in soybean oil waste water is carried out breakdown of emulsion:
Take the acinetobacter calcoaceticus bacteria suspension that 10～20mL step (1) obtains and put into the soybean oil waste water after 200mL oil removal processes, the pH=7.5 of the soybean oil waste water after oil removal processes is regulated with the NaOH that concentration is 0.01mol/L, 10min is quickly stirred with 300rpm with coagulation agitator, then stand 1 day in 32 DEG C of incubators, obtain the soybean oil waste water of oil emulsion breakdown of emulsion, measure the demulsification efficiency of soybean oil waste water oil emulsion；
(4) oil emulsion after breakdown of emulsion processes:
Take the microbial flocculant that 10～20mL step (2) obtains, put in the soybean oil waste water of the oil emulsion breakdown of emulsion that 200mL step (3) obtains, the pH=7.5 of the soybean oil waste water of breakdown of emulsion is regulated with the NaOH that concentration is 0.01mol/L, 2min is quickly stirred with 300rpm with coagulation agitator, then with 120rpm low rate mixing 10min, after standing 20 minutes, measure the clearance of soybean oil waste water oil emulsion and the clearance of COD.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610252750.7A CN105776573B (en) | 2016-04-22 | 2016-04-22 | To the method for soybean oil waste water emulsification oil processing |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201610252750.7A CN105776573B (en) | 2016-04-22 | 2016-04-22 | To the method for soybean oil waste water emulsification oil processing |
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| CN102533562A (en) * | 2011-09-27 | 2012-07-04 | 江苏科技大学 | Rhizopus oryzae TY GF1 bacterial strain and application thereof in degrading high-concentration oil in catering waste water |
| CN203866068U (en) * | 2014-04-28 | 2014-10-08 | 南通海珥玛植物油脂有限公司 | Oil-water separating device used for epoxidized soybean oil production |
| JP2015180194A (en) * | 2014-03-03 | 2015-10-15 | 国立大学法人宇都宮大学 | Microbiologic agent, microorganism flock and method for producing the same |
| CN105130108A (en) * | 2015-08-20 | 2015-12-09 | 南通海珥玛科技股份有限公司 | Epoxidized soybean oil wastewater processing technology |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102533562A (en) * | 2011-09-27 | 2012-07-04 | 江苏科技大学 | Rhizopus oryzae TY GF1 bacterial strain and application thereof in degrading high-concentration oil in catering waste water |
| JP2015180194A (en) * | 2014-03-03 | 2015-10-15 | 国立大学法人宇都宮大学 | Microbiologic agent, microorganism flock and method for producing the same |
| CN203866068U (en) * | 2014-04-28 | 2014-10-08 | 南通海珥玛植物油脂有限公司 | Oil-water separating device used for epoxidized soybean oil production |
| CN105130108A (en) * | 2015-08-20 | 2015-12-09 | 南通海珥玛科技股份有限公司 | Epoxidized soybean oil wastewater processing technology |
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