CN105661025A - Feed additive capable of reducing somatic cell account of dairy cow and preparation method thereof - Google Patents
Feed additive capable of reducing somatic cell account of dairy cow and preparation method thereof Download PDFInfo
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- CN105661025A CN105661025A CN201610012678.0A CN201610012678A CN105661025A CN 105661025 A CN105661025 A CN 105661025A CN 201610012678 A CN201610012678 A CN 201610012678A CN 105661025 A CN105661025 A CN 105661025A
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Abstract
The invention discloses a feed additive capable of reducing somatic cell account of dairy cow. The feed additive capable of reducing somatic cell account of dairy cow comprises the following ingredients in parts by weight: 20 parts of honeysuckle flower extract and eucommia bark extract, 15 parts of small peptides for feed, 50 parts of yeast for feed and 30 parts of lactic acid bacteria for feed. Usage method of the feed additive capable of reducing somatic cell account of dairy cow comprises the following steps: fully mixing 20 parts of honeysuckle flower extract and eucommia bark extract, 15 parts of small peptides for feed, 50 parts of yeast for feed and 30 parts of lactic acid bacteria for feed; sieving the mixture by a 80-mesh sieve according to feed grades; uniformly mixing the sieved mixture; and adding the sieved mixture into compound feeds in three gradient addition amounts, including 0.01%, 0.03% and 0.05%. The feed additive for improving dairy cow breast health by reducing somatic cell accounts of the dairy cows provided by the invention has obvious effects of preventing and treating mastitis of the dairy cows. Thus, the feed additive is capable of promoting breast health of the dairy cows and improving qualities of the milk products.
Description
Technical field
The invention belongs to livestock-raising technical field, particularly relate to a kind of reduction Contents in Cows cell number feed additive and preparation method thereof.
Background technology
Somatic number (somaticcellcount, SCC) is the important indicator of the udder health degree of the individual milch cow of reflection, and it determines the yield of milk, composition, quality and resting period. Latent mammitis makes milk yield reduce by 10%~20%, and fat, protein, dry matter content all decline, and total number of bacteria, somatic number significantly rise. When mammary gland tissue is subject to courses of infection, body immune system will secrete substantial amounts of immunocyte to remove infection, thus causing that in raw milk, somatic number significantly raises. There is certain change in yield and the composition that can cause raw milk further. Milk cow health is not only closely related with milk yield, vitality of giving milk, breeding potential and service life etc., and affects safety and the quality of raw material milk, directly affects the benefit of cattle farm and the health of consumer. Mastitis is the common cystic hyperplasia of breast of milch cow, be also the most popular in raising dairy cattle, endanger maximum, cause damage one of the most serious disease. According to the incomplete statistics of international milch cow community, having 10%~30% to suffer from various types of mastitis in the whole world 2.2 hundred million more bovine cows, the sickness rate of most countries mammitis of cow reaches 25%~60%. The dissimilar raiser in China Beijing area is investigated discovery by Wu Meiling etc., and the bovine subclinical mastitis sickness rate of casual household is 43.01%, and the sickness rate of plant is 45.37%, and the sickness rate of Animal Raising Zone is 49.21%; The sickness rate of the first-born cattle latent mammitis is 39.29%, and the sickness rate of two tire cattle is 41.36%, and the sickness rate of triplet cattle is 45.77%, and the sickness rate of four tires and above cattle is 58.33%. Mastitis causes milk to abandon, and cow is eliminated too early, and artificial nursing cost and veterinary spend increase, bring huge economic loss to milk industry. According to incompletely statistics, in worldwide, the loss caused because of mammitis of cow every year is up to 35,000,000,000 dollars. The economic loss that China causes because of all kinds mastitis every year is approximately 5,000,000,000 yuans, and presents the trend of average annual growth.It addition, suffer from the milch cow of mastitis, containing substantial amounts of toxin and pathogenic microorganism in its milk, being eaten by people if come into the market, can cause vomiting, suffer from diarrhoea, generating heat and the symptom such as allergy, seriously jeopardizing the health of consumer. As can be seen here, or even mammitis of cow is the disease that a kind of sickness rate is high, economic loss is big, hazardness is strong, causes serious loss to whole world milk industry, it is necessary to cause showing great attention to and paying attention to of experts and scholars, raising dairy cattle personnel, ordinary consumer.
The treatment of mastitis relies primarily on antibiotherapy for a long time, and however as antibiotic a large amount of uses, the negative issue such as drug residue, pathogenic bacteria drug resistance is increasingly severe.
Summary of the invention
It is an object of the invention to provide a kind of reduction Contents in Cows cell number feed additive, aim to solve the problem that the treatment of mastitis relies primarily on antibiotherapy for a long time, however as antibiotic a large amount of uses, drug residue, pathogenic bacteria drug resistance negative issue are increasingly severe.
The present invention is realized in, a kind of reduction Contents in Cows cell number feed additive, described reduction Contents in Cows cell number feed additive includes in mass parts: Flos Lonicerae extract and Cortex Eucommiae extract 20 parts, the little peptide of feedstuff 15 parts, feed yeast 50 parts, feedstuff lactic acid bacteria 30 parts.
The using method of described reduction Contents in Cows cell number feed additive includes:
Flos Lonicerae extract and Cortex Eucommiae extract 20 parts, the little peptide of feedstuff 15 parts, feed yeast 50 parts, after feedstuff lactic acid bacteria 30 parts is sufficiently stirred for, according to feedstuff rank, cross 80 mesh sieves, stir, add in mixed feed by 0.01%, 0.03% and 0.05% 3 gradient addition.
Enhancing milch cow immunity provided by the invention, promotion gastrointestinal health, and then reduce Contents in Cows cell number, improve the feed additive that cow breast is healthy, preventing and treating mammitis of cow is had a significant effect, thus reaching to promote that cow breast is healthy, improve the purpose of Quality of Dairy Products, provide safe and reliable milk supply for consumers in general.
Accompanying drawing explanation
Fig. 1 is the using method flow chart reducing Contents in Cows cell number feed additive that the embodiment of the present invention provides.
Detailed description of the invention
In order to make the purpose of the present invention, technical scheme and advantage clearly understand, below in conjunction with embodiment, the present invention is further elaborated. Should be appreciated that specific embodiment described herein is only in order to explain the present invention, is not intended to limit the present invention.
The present invention is comprehensively analyzing effective ingredient and the mechanisms of action such as Cortex Eucommiae extract, Flos Lonicerae extract, antibacterial peptide, yeast culture, cultures of lactobacilli, and on the basis of dairy cow nutrition demand, obtain and reduce the feed addictive prescription of somatic number in milk; Then according to 3 gradients are added, test at two and cattle farm 300 cow head has carried out bimestrial feeding experiment by a definite date, in result matched group milk, somatic number pole significantly reduces (P < 0.01), illustrates that preventing and treating mammitis of cow is had a significant effect by novel fodder formula used in the present invention.
Below in conjunction with accompanying drawing, the application principle of the present invention is explained in detail.
The reduction Contents in Cows cell number feedstuff of the embodiment of the present invention includes: include in mass parts: Flos Lonicerae extract and Cortex Eucommiae extract 20 parts, the little peptide of feedstuff 15 parts, feed yeast 50 parts, feedstuff lactic acid bacteria 30 parts.
As it is shown in figure 1, the using method reducing Contents in Cows cell number feed additive of the embodiment of the present invention comprises the following steps:
S101: Flos Lonicerae extract and Cortex Eucommiae extract 20 parts, the little peptide of feedstuff 15 parts, feed yeast 50 parts, after feedstuff lactic acid bacteria 30 parts is sufficiently stirred for;
S102: according to feedstuff rank, crosses 80 mesh sieves, stirs, add in mixed feed by 0.01%, 0.03% and 0.05% 3 gradient addition.
Flos Lonicerae and Cortex Eucommiae extract: purchase the happiness 100 (containing Flos Lonicerae and Cortex Eucommiae extract) of Shandong Long Chang animal health-care product company limited. Little peptide (feed additive) purchased from Beijing Zhongnong Yingnong Bioisystech Co., Ltd, feed yeast purchased from Shenzhen Sunsmile Biotechnology Co., Ltd., cultures of lactobacilli purchased from Beijing Zhongnong Yingnong Bioisystech Co., Ltd. 80 mesh sieves are crossed for reagent product. The dry place of pack saves backup.
Below in conjunction with concrete pharmacological analysis, the application principle of the present invention is further described.
The Cortex Eucommiae is as a kind of traditional rare Chinese medicine, in China's cultivation history of existing more than 2,000 year. Containing lignanoids, iridoid glycosides, Phenylpropanoid Glycosides class, flavonoid and phenolic acid in Folium Eucommiae, skin, there is the multiple efficacies such as invigorating the liver and kidney, anti-inflammation, enhancing immunity. Its contained polyhydroxy phenol can suppress α-maltase activity, and then suppresses the growth of the pathogenic microorganisms such as antibacterial, fungus and virus; Escherichia coli, staphylococcus aureus, streptococcus pneumoniae and anthrax bacillus etc. are had inhibitory action in various degree by chlorogenic acid; Iridoid glycosides can by providing proton hydrogen to obtain scavenging free radicals and antioxidation; Polysaccharide can pass through excited reticuloendothelial system enhancing body non-specific immunity. As a kind of novel additive feedstuff, the Cortex Eucommiae has been widely used in the animal productiong such as beef cattle, goat, sheep, broiler, laying hen, pig, and effect is safe and reliable, noresidue, has no side effect. The report Cortex Eucommiae extract such as Yao's big shield can significantly improve butterfat percnetage, milk sugar rate and reduction milch cow protein ratio. Cortex Eucommiae extract can significantly improve IgG content in milch cow conception rate, milk butterfat percnetage, blood, reduces milk somatic cell number, protein ratio, blood malonaldehyde level and the effect of prevention mastitis. Cortex Eucommiae extract can improve cow rumen environment to a certain extent, improves the cud utilization rate to nitrogen, the liver protecting, improves immunity of organisms and metaboilic level. Correlational study all shows, it is notable that Cortex Eucommiae extract is applied in Cow product effect as feed additive.
Flos Lonicerae is as the conventional Chinese herbal medicine of one, and applicating history is long. It has the effects such as heat-clearing and toxic substances removing, wind-dispelling heat-dissipating, hepatic cholagogic, immunomodulating, anti-inflammation, its antimicrobial component is mainly organic acid compound, including chlorogenic acid, neochlorogenic acid, 4-dicaffeoylquinic acid, 3,4-dicaffeoyl quinic acid, 3,5-dicaffeoyl quinic acid, 4,5-DCQA etc. Flos Lonicerae is widely used in Animal Medicine preparation with its good anti inflammatory immunity effect. Main pathogen of bovine mastitis is had certain bacteriostasis by the Chinese medicine preparation containing 5 taste Chinese herbal medicine such as Flos Loniceraes. The 12 taste herbs in vitro bacteriostatic activities such as 26 taste heat-clearing and detoxifying herbs are suppressed relatively showing of mastitis pathogen least concentration by Wan Feng etc., Flos Lonicerae are stronger. Zhang Qidi etc., filtering out the 5 taste Chinese herbal medicine comprising Flos Lonicerae, develop the compound honeysuckle injection for mammitis of cow, and go safety to test it. At present, Flos Lonicerae joins in Animal Medicine preparation as antibacterials and is widely used, but is as in feed additive addition milk cow forage and have not been reported.
Antibacterial peptide is the active small molecular polypeptide of the class opposing pathogen invasion that organism immune system produces under inductive condition.It kills the integrity of action breaks down pathogen cell membrane by film, causes intracellular organic matter to overflow thus reaching the effect of pathogenic microbe killing. Experiment in vitro shows, antibacterial peptide has broad-spectrum antibacterial action, it is possible to kills or suppresses the common pathogens such as escherichia coli, bacillus pyocyaneus, streptococcus, staphylococcus, and be not likely to produce Drug resistance, it does not have drug residue. Antibacterial peptide is added in milk cow forage as functional feedstuff additive, will effectively prevent and treat mammitis of cow.
Yeast culture is a kind of microbial ecological agent, refers to that yeast controls in the liquid of condition, solid second order fermentation or direct concrete culture medium after fermentation strict, the product being processed into together with culture medium. It includes the culture medium three part composition after yeast cells, extracellular metabolite and fermentation, rich in vitamin, mineral, somatomedin, polysaccharide, digestive enzyme and aminoacid, there is the effect promoting growth, enhancing human body immunity. Yeast culture carrys out enhancing human body immunity power mainly through the effect of two aspects: stimulating immune system generation immunne response on the one hand, safeguards that there is a function in the normal face of body; Pathogen is adhered on the one hand, it is prevented that the absorption of toxin and refuse by mannan etc. At present, yeast culture is increasingly subject to people's attention, and has been widely used for cow feed additive.
Lactobacillus is the normal dominant microflora in animal and bird intestines, and it has important immunologic function. It can pass through to produce the materials such as organic acid, biacetyl, peroxide, improve the internal microenvironment of gastrointestinal tract of livestock and fowls, reduce pH value and suppress the growth of the harmful bacteria such as putrefaction bacteria and pathogen, chicken pathogenic escherichia coli is had obvious inhibitory action by lactic acid bacteria culture, lactobacillus acidophilus's culture has the effect of prevention white scour of chicken bacillosis, lactic acid factor pair escherichia coli in cultures of lactobacilli have toxic action, can suppress colibacillary growth. Current cultures of lactobacilli is used primarily in chicken feed addictive, adds cultures of lactobacilli and rarely have report in dairy cow diet.
The effect that the materials such as Cortex Eucommiae extract, Flos Lonicerae extract, antibacterial peptide, yeast culture, cultures of lactobacilli are respectively provided with enhancing human body immunity power, promote body gastrointestinal tract healthy, is all widely used in feed additive or pharmaceutical preparation. But these benefit materials are arranged in pairs or groups with rational ratio, the feed additive as anti-mammitis of cow have not been reported. The present invention, comprehensively analyzing on the basis of above each material effective ingredient, according to dairy cow nutrition health demand, has prepared respective additive formula and ratio, has finally given milch cow and has reduced somatic number feed addictive prescription. This formula verifies have excellent effect, significantly reduce somatic number content in milk, promoted the health of cow breast, improve yield and the component content in milk of milk through feeding experiment.
Below in conjunction with concrete experiment, the application effect of the present invention is further described.
1 material and method
1.1 experimental animals
Select in Song Cun cattle farm, Hongya County, Meishan city and Pu Zhou cattle farm, Ziyang City, Sichuan Province Anyue County respectively milch cow that body condition, parity, lactation period, milk yield, milk composition are close totally 300 test. It is equally divided into 4 groups, often 3 repetitions of group. Test cattle all has relevant breeding and the record of production.
1.2 test methods
The cattle stochastic averagina deriving from same cattle farm being divided into two groups, i.e. test group and matched group, the cattle number of cattle often organized in record. Matched group is the basal diet without addO-on therapy, and test group adds 3 gradients for examination component formula on the basis of basal diet.In same field, basal diet, feeding and management method and milking processes are all identical. Preliminary trial period is 10 days, and the formal test phase is 2 months.
All experiment cattle basal diet structures are mixed feed 10Kg, Alfalfa 4Kg, dried wild grass 2Kg, Silage Corn Straw 10Kg. Concentrate mixture crude protein >=19%, NEL 34.73MJ, digestible crude protein matter 293g.
The milk production trait measured mainly includes somatic number, butterfat percnetage, protein ratio, daily yielding and free fatty. Somatic number and galactopoiesis achievement data are by ADAM-MC full-automatic cell calculating instrument and the detection of FT120 analysis of milk composition instrument. Raw milk free fatty acid (freefattyacid, FFA) assay adopts Copoloid method, and its normal equation is y=380.77x+5.8495 (R2=0.9927), in formula: x is absorbance, y is FFA content/(μm ol/L).
1.3 data analysiss
Test data, after Excel edit, adopts SAS9.2 software to carry out two-way analysis of variance, and adopts SSR method to carry out multiple comparisons. Statistical model is:
Yijk=u+Hi+Gj+Eijk
Yijk, milk composition observation;
U, colony's average;
Hi, cattle farm fixed effect, i=1,2;
Gj, test packet fixed effect, j=1,2;
Eijk, random residual effect.
2 results and analysis
The different concentrate feed impact on milk somatic cell number of table 1
As shown in Table 1, before test, difference not notable (P>0.05) between matched group and test group somatic number; After test, between matched group and test group somatic number, difference reaches pole significant level (P<0.01), and matched group somatic number is significantly higher than test group somatic number (P<0.01), and test difference not notable (P>0.05) between each group; Before and after test, between matched group somatic number, difference reaches pole significant level (P<0.01), and after test, before matched group somatic number is significantly higher than test; Before and after test, difference not notable (P>0.05) between test group somatic number. Illustrating that with the addition of additive in feedstuff is effectively controlled the growth of Contents in Cows cell number, namely feed additive has promoted that cow breast is healthy, effectively prevents the generation of mastitis.
Table 2 tests each group of milk yield and analysis of milk composition table
As can be seen from Table 2, compared with matched group, test each index and all there occurs change, and daily yielding and protein ratio there occurs notable change. From daily yielding, after matched group test, daily yielding substantially reduces, but not notable. Test each group of daily yielding compared with matched group, significant difference (P<0.05), and 2,3 groups of effects of experiment are best. Difference not notable (P>0.05) before butterfat percnetage experiment. But each group numerical value all slightly rises after experiment. From protein ratio, test each group of numerical value and all rise to some extent, but test each group compared with matched group protein ratio significantly raise (P<0.05). Before and after matched group experiment, free fatty rises to some extent, but tests each group of free fatty and decline to some extent, illustrates that milk quality is better, not easily corrupt, is more easy to preservation.
It can thus be seen that feed this feed additive can significantly improve milk production of cow and milk composition, meanwhile, milk product free fatty acid is few, not easily corrupt, is more easy to preservation. Experiment 2,3 groups is praised highly in this research, effective, zero difference. But from reducing the angle of cost, the addition of test 2 is worthy to be popularized.
The foregoing is only presently preferred embodiments of the present invention, not in order to limit the present invention, all any amendment, equivalent replacement and improvement etc. made within the spirit and principles in the present invention, should be included within protection scope of the present invention.
Claims (2)
1. one kind is reduced Contents in Cows cell number feed additive, it is characterized in that, described reduction Contents in Cows cell number feed additive includes in mass parts: Flos Lonicerae extract and Cortex Eucommiae extract 20 parts, the little peptide of feedstuff 15 parts, feed yeast 50 parts, feedstuff lactic acid bacteria 30 parts.
2. reduce Contents in Cows cell number feed additive as claimed in claim 1, it is characterised in that the using method of described reduction Contents in Cows cell number feed additive includes:
Flos Lonicerae extract and Cortex Eucommiae extract 20 parts, the little peptide of feedstuff 15 parts, feed yeast 50 parts, after feedstuff lactic acid bacteria 30 parts is sufficiently stirred for, according to feedstuff rank, cross 80 mesh sieves, stir, add in mixed feed by 0.01%, 0.03% and 0.05% 3 gradient addition.
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Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
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CN106173276A (en) * | 2016-07-18 | 2016-12-07 | 柳州市柳南区安顺养殖协会 | A kind of preparation method of cow feed additive |
CN106615740A (en) * | 2016-12-20 | 2017-05-10 | 新昌县柏克动物饲料技术开发有限公司 | Feed additive capable of reducing number of somatic cells in milk |
CN106858053A (en) * | 2016-12-27 | 2017-06-20 | 新昌县拜瑞珂生物技术有限公司 | The feed addictive of somatic number and its application in a kind of reduction milk |
CN107950783A (en) * | 2017-12-12 | 2018-04-24 | 湖南德人牧业科技有限公司 | A kind of fine fodder for preventing mastitis for milk cows and preparation method thereof, equipment |
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CN104171432A (en) * | 2014-08-18 | 2014-12-03 | 北京农业职业学院 | Compound biological feed additive for dairy cows and preparation method of compound biological feed additive for dairy cows |
CN104474006A (en) * | 2014-11-29 | 2015-04-01 | 郑州后羿制药有限公司 | Traditional Chinese medicine microecological preparation capable of increasing lactation yield of dairy cow and preparation method thereof |
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CN1853497A (en) * | 2005-04-20 | 2006-11-01 | 上海泓宝绿色水产科技发展有限公司 | Improvement of cow milk output and milk quality by Chinese medicinal herb composite microbial forage additive |
CN104171432A (en) * | 2014-08-18 | 2014-12-03 | 北京农业职业学院 | Compound biological feed additive for dairy cows and preparation method of compound biological feed additive for dairy cows |
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Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN106173276A (en) * | 2016-07-18 | 2016-12-07 | 柳州市柳南区安顺养殖协会 | A kind of preparation method of cow feed additive |
CN106615740A (en) * | 2016-12-20 | 2017-05-10 | 新昌县柏克动物饲料技术开发有限公司 | Feed additive capable of reducing number of somatic cells in milk |
CN106858053A (en) * | 2016-12-27 | 2017-06-20 | 新昌县拜瑞珂生物技术有限公司 | The feed addictive of somatic number and its application in a kind of reduction milk |
CN107950783A (en) * | 2017-12-12 | 2018-04-24 | 湖南德人牧业科技有限公司 | A kind of fine fodder for preventing mastitis for milk cows and preparation method thereof, equipment |
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