CN105503288A - Method for preparing EM bio-organic fertilizer by use of biogass slurry and bio-organic fertilizer - Google Patents
Method for preparing EM bio-organic fertilizer by use of biogass slurry and bio-organic fertilizer Download PDFInfo
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- CN105503288A CN105503288A CN201610072230.8A CN201610072230A CN105503288A CN 105503288 A CN105503288 A CN 105503288A CN 201610072230 A CN201610072230 A CN 201610072230A CN 105503288 A CN105503288 A CN 105503288A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F7/00—Fertilisers from waste water, sewage sludge, sea slime, ooze or similar masses
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D9/00—Other inorganic fertilisers
- C05D9/02—Other inorganic fertilisers containing trace elements
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/40—Treatment of liquids or slurries
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F9/00—Fertilisers from household or town refuse
- C05F9/04—Biological compost
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/20—Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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Abstract
The invention provides a method for preparing an EM bio-organic fertilizer by use of biogass slurry. The method comprises the following steps: adding an EM stock solution and an EM nutrient fluid into biogass slurry, and cultivating the biogas slurry by EM to obtain a bio-organic fertilizer, wherein the mass ratio of biogas slurry, the EM stock solution and the EM nutrient fluid is (95-105): (2-6): (0.5-1.5). As the EM cultivation mode is adopted, the method for preparing the EM bio-organic fertilizer by use of biogass slurry has the advantages of being convenient, simple, easy to operate and the like. The bio-organic fertilizer contains rich organic matters and lots of beneficial bio-funguses, can play a soil improvement role, can accelerate decomposition of the organic matters, has the advantages of being rich in nutrition, being easy to be absorbed by plants, improving the yield and the quality of crops and the like, is a leaf fertilizer with comprehensive nutrients and can treat and control some plant diseases and insect pests of the crops.
Description
Technical field
The present invention relates to fertilizer field, the method utilizing natural pond liquid to prepare EM bacteria organic fertilizer in particular to a kind of and this organic fertilizer.
Background technology
At present, most domestic technology just carries out anaerobically fermenting animal excrement and stalk, and produce methane gas and collect burning or generating use, natural pond slag, natural pond liquid are not recycled.The threat of natural pond liquid inherently water pollution, natural pond liquid directly enters soil and water, can have potential Pollution risk to periphery surface water and groundwater.Data is had to show, after natural pond liquid is directly applied to soil, although soil can adsorb a part of ammonium nitrogen, most of ammonium nitrogen still can infiltrate in lower water layer and lower infiltration with current, polluted by nitrogen is caused to soil and groundwater, great hidden danger is caused to body eutrophication.
Can discharge after natural pond slag and natural pond liquid need sewage disposal up to standard, and sewage disposal expense very expensive (expense of process natural pond liquid is about 5 yuan/ton), natural pond liquid directly discharge can pollute surrounding enviroment.
In view of this, special proposition the present invention.
Summary of the invention
The first object of the present invention is to provide a kind of method utilizing natural pond liquid to prepare EM bacteria organic fertilizer, to solve existing natural pond liquid meeting pollution on the environment, and the problem avoiding the natural pond liquid of high cost to reclaim, the described natural pond liquid that utilizes prepares the method for EM bacteria organic fertilizer, adopt the mode that EM bacterium is cultivated, the method have conveniently, the advantage such as simple, easy handling.
The second object of the present invention is to provide a kind of described natural pond liquid that utilizes to prepare biological organic fertilizer prepared by the method for EM bacteria organic fertilizer, and this biological organic fertilizer contains abundant organic matter and a large amount of beneficial organism bacterium, has the effect of improvement soil.There is the advantages such as nutritious, yield and quality that is that be easily absorbed by plants, can improve farm crop.Be the comprehensive foliage fertilizer of a kind of nutrient, can be used for all farm crop, fruit and flowers.In addition, this organic fertilizer can also prevent and treat some diseases and pests of agronomic crop.
In order to realize above-mentioned purpose of the present invention, spy by the following technical solutions:
Utilize natural pond liquid to prepare a method for EM bacteria organic fertilizer, comprise the following steps:
Add in the liquid of natural pond by EM bacterium stoste and EM bacterial nutrient solution, natural pond liquid, after EM bacterium is cultivated, obtains biological organic fertilizer;
Wherein, the mass ratio of natural pond liquid, EM bacterium stoste and EM bacterial nutrient solution is (95-105): (2-6): (0.5-1.5).
Natural pond liquid mostly is cow dung, rabbit excrement, chicken manure, pig manure mixed fermentation form, tasteless after fermentation, and the fertilizer efficiency after fermentation is more than 10 times of general chemistry synthetic fertilizer.Containing abundant organic matter, nitrogen, phosphorus, potassium, each amino acid, VITAMIN, protein, Plant hormones regulators,gibberellins, growth hormone, carbohydrate, nucleic acid and microbiotic etc. in the liquid of natural pond, and, also containing butyric acid, indolylacetic acid, vitamin B12 isoreactivity resisting substance.Therefore, the dual function promoting plant growth and control disease generation is had.Natural pond liquid is water quality characteristic, is very easily absorbed by plants, can not as chemical fertilizer, and natural pond liquid usage quantity too much also can not burn seedling.
EM is the abbreviation language of English (EffectiveMicroorganisms), and Chinese is translated into " effective microorganism ".EM bacterium be based on photosynthetic bacterium, milk-acid bacteria, yeast and actinomycetes 10 belong to more than 80 and plant the micro-life bacteria preparation of one that microorganism is composited.The mechanism of action is the competition forming EM bacterium and pathogenic micro-organism contention nutrition, due to EM bacterium very easily survival and reproduction in soil, so the ecologic niche in soil comparatively fast and stably can be occupied, form the advantage group of useful microbial bacteria, thus control the breeding of pathogenic micro-organism and the invasion and attack to crop.EM bacterial nutrient solution mainly provides essential nutrition for the breeding of EM bacterium, promotes that EM bacterium breeds rapidly.
Natural pond liquid is organic fertilizer that is water-soluble, quick-acting high-quality, but concentration is lower, each large usage quantity.In the liquid of natural pond, add EM bacterium stoste, be a kind of Harmless process.Tame unhelpful flora with probiotics, make natural pond liquid while containing abundant organic matter (protein, amino acid, carbohydrate and nucleic acid), also containing abundant probiotic bacterium.Probiotic bacterium can promote organic matter decomposition, and fertilizer is very easily absorbed by plants.Natural pond liquid after method process provided by the invention, nutrition content is higher, can provide more energy, nutriment and microorganism for soil microbial activity.Meanwhile, microbial activities can be promoted, accelerate organic matter decomposition.Further, this organic fertilizer is more suitable for the growth demand of farm crop, and the active substance in fertilizer etc. can promote the growth of crop and improve the quality of agricultural-food.Also avoid most of ammonium nitrogen to infiltrate in lower water layer and lower infiltration with current, polluted by nitrogen is caused to soil and groundwater, great hidden danger is caused to body eutrophication.
Natural pond provided by the present invention liquid, is applicable to organic natural pond liquid such as natural pond, various livestock-raising field liquid, feed-processing plant's natural pond liquid, Gourmet Powder Factory's natural pond liquid, food processing plant's natural pond liquid, Amino Acid Factory.
Preferably, the described natural pond liquid that utilizes prepares the method for EM bacteria organic fertilizer, specifically comprises the following steps:
1), by natural pond liquid carry out isolation and identification through ultra-filtration membrane and reverse osmosis membrane, obtain concentrated natural pond liquid and water, and natural pond liquid disinfection will be concentrated;
2), in step 1) in sterilization after concentrated natural pond liquid in add EM bacterium stoste and EM bacterial nutrient solution, carry out 7-30 days EM bacterium cultivate and stir, add trace element after cultivation completes and carry out solid-liquid separation, obtaining solid-state and liquid biological organic fertilizer respectively;
Wherein, the mass ratio of natural pond liquid, EM bacterium stoste and EM bacterial nutrient solution is (98-102): (3-5): (0.8-1.2).
First, ultra-filtration and separation is carried out with ultra-filtration membrane.Ultrafiltration take pressure difference as the liquid phase membrane separating process of impellent.It has been generally acknowledged that molecular weight solute is higher than 500d, respective aperture size is the sepn process of 10-100nm is ultrafiltration.For ultrafiltration, Screening theory is widely used for analyzing its separating mechanism.This theory is thought, film surface has numerous micropore, the hole of these in esse different pore sizes, as sieve, retains solute and particle that molecular diameter is greater than aperture, thus reaches the object of separation.Ultra-filtration membrane, be that a kind of aperture specification is consistent, nominal pore scope is the micropore filtering film of 0.001-0.02 micron.Impose suitable pressure in the side of film, just can sift out the solute molecule being less than aperture, with the particle that isolated molecule amount is greater than 500 dalton (atomic mass unit), particle diameter is greater than 10 nanometers.
Then, reverse osmosis is carried out with reverse osmosis membrane.Reverse osmosis, also known as reverse osmosis, be a kind of is impellent with pressure difference, isolates the membrane sepn operation of solvent from solution.Because it is contrary with the direction of naturally osmotic, therefore claims reverse osmosis.According to the different osmotic of various material, just can use the reverse osmosis pressure being greater than osmotic pressure, i.e. reverse osmosis method, reach separation, extraction, purifying and concentrated object.Reverse osmosis membrane is the core parts realizing reverse osmosis, is a kind of artificial semi-permeable membranes with certain characteristic of simulating biological semi-permeable film and making.Employing macromolecular material is made: as one or several in cellulose acetate membrane, aromatic polyhydrazide film, aromatic polyamide film combine.The diameter of surface micropore is generally between 0.5-10nm, and the size of perviousness is relevant with the chemical structure of film itself.Some macromolecular materials are good to the repellency of salt, and the penetration speed of water bad.Some high polymer material chemical structures have more hydrophilic radical, and thus the penetration speed of water is relatively very fast.Effectively can be separated clear water and natural pond liquid.
After ultrafiltration and reverse osmosis, have the clear water of 30-50%, this part clear water can reuse, is used between cleaning Vehicle, flushes the toilet and pouring etc.The concentrated natural pond liquid of residue 50-70% can proceed the cultivation of EM bacterium.In the liquid of natural pond, parasitic ovum and various pathogenic bacteria are almost killed at anaerobically fermenting for more than 30 days.But, in order to prevent and treat intestinal bacteria with some worm's ovums to the impact of biological organic fertilizer, concentrated natural pond liquid is before entering EM bacterium breeding phase, and also need disinfection, described sterilization method can be: the one in infrared sterilization, disinfection by ultraviolet light or ozonization.Then, the concentrated natural pond liquid after sterilization is cultivated, cultivate 7-10 days.
Natural pond liquid after EM bacterium is cultivated, is organic fertilizer.After solid-liquid separation, natural pond liquid and natural pond slag all can be used as organic fertilizer.Natural pond liquid can be used as field irrigation and use, and natural pond slag can direct effect and plant root.
Preferably, described EM bacterium stoste comprises: containing one or several the bacterium liquid in the thread flora that photosynthetic bacteria group, lactobacillus, yeast flora, Gram positive actinomycetes group and fermentation are.
Photosynthetic bacteria group has aerobic and anaerobies, as photosynthetic bacterium and blue algae.There is photosynthesis and nitrogen fixation.Belong to autotrophy microorganism, can self duplication.Thalline itself contains the protein of more than 60%, and is rich in multivitamin, also containing Coenzyme Q10 99.0, antiviral substance and somatomedin; It with soil accept light and heat for the energy, sulphur hydrogen in soil and the hydrogen in hydrocarbon polymer are separated, change objectionable impurities is innoxious substance, and with the organism in the secretory product of plant root, soil, obnoxious flavour (hydrogen sulfide etc.) and carbonic acid gas, nitrogen etc. for matrix, synthesis carbohydrate, amino acids, vitamins, nitrogen compound, antiviral substance and physiologically active substance etc.These materials are all rich soil and the important force promoting animal and plant growth.
The metabolic substd of photosynthetic bacteria group directly can be absorbed by plant, can also become the nutrient of other microbial reproduction.If photosynthetic bacterium breeds, other beneficial microorganism also can be bred.Such as, the amino acid that VA VA Mycorrhizal Fungi is secreted with photosynthetic bacteria is for bait, and it can dissolve insoluble phosphorus, again can with vinelandii symbiosis, its nitrogen fixing capacity is significantly improved.
Lactobacillus has anaerobies.Take as the leading factor with Lactobacterium acidophilum, the carbohydrate that it produces by picked-up photosynthetic bacterium, yeast forms lactic acid.Lactic acid has very strong sterilizing ability, effectively can suppress the movable and organic sharply corrupt decomposition of harmful microbe.Milk-acid bacteria can decompose not labile xylogen and Mierocrystalline cellulose under normal conditions, and eliminates all drawbacks of undecomposed organism generation; Synthesize each seed amino acid, VITAMIN, produce digestive ferment, enhance metabolism, melt the ability of insoluble inorganic phosphorus in addition.
The pathogenic bacterium propagation that milk-acid bacteria can also suppress continuous cropping obstacle to produce.Pathogenic bacterium enliven, and harmful nematode can sharply increase, and plant will be weak, and milk-acid bacteria inhibits pathogenic bacterium, and harmful nematode just can fade away.Therefore, EM bacteria organic fertilizer prepared by the method, can also prevent and treat some diseases and pests of agronomic crop.
Yeast flora has aerobic.The amino acid that the secretory product that it utilizes plant root to produce, photosynthetic bacteria synthesize, carbohydrate and other organic substance produce fermenting power, and synthesis promotes root growth and fissional sensitization material.Yeast provides important subsistence support for the matrix (food) promoted required for other effective microbe (as milk-acid bacteria, actinomycetes) propagation in EM original solution.In addition, the single cell protein that yeast produces is the indispensable nutrient of animal.
Gram positive actinomycetes group has aerobic.It obtains amino acid, nitrogen etc. as matrix from photosynthetic bacterium, produces various antibiotics, VITAMIN and enzyme, directly can suppress pathogenic bacteria.It obtains the matrix required for harmful mould and bacterial multiplication in advance, thus suppresses their propagation, and creates the living environment of other beneficial microorganism propagation.Actinomycetes and the mixed germicidal action of photosynthetic bacterium, more much bigger than the lethality of actinomycetes individual combat.The material that it decomposes difficulty, as xylogen, Mierocrystalline cellulose, chitin etc. have Degradation, and easy passive plant absorption, strengthen animals and plants to the resistibility of various disease and immunizing power.Actinomycetes also can promote vinelandii and VA VA Mycorrhizal Fungi propagation.
The thread flora of fermentation system has anaerobies.Based on the Eurotium used during fermentation alcohol, it can coexist with other microorganisms, especially has good result to the generation of the ester in soil.Because alcohol producing power is strong, alcohol can prevent and treat maggot and other insects, and can eliminate stench.Therefore, EM bacteria organic fertilizer prepared by the method, can also prevent and treat some diseases and pests of agronomic crop.
The composite bacterial solution of a kind of flora in above flora or several flora is adopted in the present invention.Adopt this combination bacterium to cultivate concentrated natural pond liquid, prepared biological organic fertilizer, there is improvement soil, strengthen photosynthesis, improve water quality, deodorizing excrement, growth promotion, the effect such as disease-resistant, antibacterial.
Preferably, described EM bacterial nutrient solution comprises: brown sugar water and/or G/W.
Multiple monose and the polyose energy matter such as glucose, fructose contained in brown sugar, can accelerate the metabolism of skin cells, for cell provides energy.And glucose can provide microbial metabolism necessary energy.
Preferably, in step 2) in, the temperature of described cultivation is 5-25 DEG C.
At this temperature, be applicable to the breeding of EM bacterium and cultivate, preventing neutralize bacteria, inactivation.
Preferably, in step 2) in, described EM bacterium stoste is divided 1-3 time and is added.
Progressively add EM bacterium, effectively control its breeding and cultivate, and ensureing that EM bacterium fully contacts with material.
Preferably, in step 2) in, the described one stirred in employing submersible water impeller, diving reflux pump or employing blower fan.
Stirring can also ensure when cultivating while natural pond liquid can be made fully to contact with material with EM, can take a breath fully, ensures the breeding of EM bacterium.Submersible water impeller, diving reflux pump can carry out return stirring to natural pond liquid; And blower fan carries out intermittent aeration, thus play the effect of stirring.
Preferably, in step 2) in, described trace element is one or more in selenium, boron, zinc, manganese, molybdenum, copper, iron, cobalt.
Trace element, refers to the fertilizer containing micronutrient element, and crops absorbs consumption few (for major element fertilizer).Although crop is little to micro-requirement, they are the same with major element, be of equal importance, can not replace mutually crop.Trace element use, on the basis of nitrogen, phosphorus, potash fertilizer its fertilizer efficiency of competence exertion.In soil, any one quick-acting state trace element is under-supply, and crop just there will be special symptom, and output reduces, and quality declines, and even harvest is hopeless.
Preferably, in step 2) in, described solid-liquid separation adopts 80-120 order plate-and-frame filter press to carry out.
Adopt 80-120 order plate-and-frame filter press to carry out solid-liquid separation to the fertilizer cultivated, when filling with field to prevent this biological organic fertilizer, blocking instrument, like this, solid and liquid biological organic fertilizer can be suited measures to local conditions, and use respectively.
The biological organic fertilizer that a kind of described method utilizing natural pond liquid to prepare EM bacteria organic fertilizer is made.
This fertilizer is applicable to that all kinds of farm crop, vegetables, melon and fruit, herbal medicine, flowers, tealeaves, nursery stock etc. are organic, green, the production of pollution-free food.
Compared with prior art, beneficial effect of the present invention is:
(1) method utilizing natural pond liquid to prepare EM bacteria organic fertilizer provided by the invention, adopts the mode that EM bacterium is cultivated, the advantages such as the method has conveniently, simple, easy handling.
(2) the natural pond liquid that utilizes provided by the invention prepares EM bacteria organic fertilizer, this biological organic fertilizer contains abundant organic matter and a large amount of beneficial organism bacterium, there is the effect of improvement soil, and more energy and nutriment can be provided for soil microbial activity, promote microbial activities, accelerate organic matter decomposition, fertilizer is more easily absorbed by plants.This biological organic fertilizer is more suitable for the growth demand of farm crop, and the active substance in fertilizer etc. can promote the growth of crop and improve the quality of agricultural-food.Be the comprehensive foliage fertilizer of a kind of nutrient, can be used for all farm crop, fruit and flowers.
(3) the natural pond liquid that utilizes provided by the invention prepares EM bacteria organic fertilizer, can prevent and treat some diseases and pests of agronomic crop, improves crop.
Embodiment
Below in conjunction with embodiment, embodiment of the present invention are described in detail, but it will be understood to those of skill in the art that the following example only for illustration of the present invention, and should not be considered as limiting the scope of the invention.Unreceipted actual conditions person in embodiment, the condition of conveniently conditioned disjunction manufacturers suggestion is carried out.Agents useful for same or the unreceipted production firm person of instrument, be and can buy by commercially available the conventional products obtained.
Embodiment 1
The method utilizing natural pond liquid to prepare EM bacteria organic fertilizer provided by the invention, comprises the following steps:
Add in the liquid of natural pond by EM bacterium stoste and EM bacterial nutrient solution, natural pond liquid, after EM bacterium is cultivated, obtains biological organic fertilizer.
Wherein, the mass ratio of natural pond liquid, EM bacterium stoste and EM bacterial nutrient solution is 95:2:0.5.
Embodiment 2
The method utilizing natural pond liquid to prepare EM bacteria organic fertilizer provided by the invention, comprises the following steps:
Add in the liquid of natural pond by EM bacterium stoste and EM bacterial nutrient solution, natural pond liquid, after EM bacterium is cultivated, obtains biological organic fertilizer.
Wherein, the mass ratio of natural pond liquid, EM bacterium stoste and EM bacterial nutrient solution is 105:6:1.5.
Embodiment 3
S31, natural pond liquid is carried out isolation and identification through ultra-filtration membrane and reverse osmosis membrane, obtain concentrated natural pond liquid and water, and natural pond liquid disinfection will be concentrated;
The EM bacterium stoste of thread flora containing photosynthetic bacteria group, lactobacillus, yeast flora, Gram positive actinomycetes group, fermentation system is added in S32, concentrated natural pond liquid in step S31 after sterilization, add the EM bacterial nutrient solution comprising brown sugar water, be under the condition of 5 DEG C in temperature, carry out 7 days EM bacterium to cultivate, and adopt submersible water impeller to carry out return stirring, add selenium, boron, zinc, manganese element after cultivation completes, and adopt 80 order plate-and-frame filter press to carry out solid-liquid separation to obtain solid-state and liquid biological organic fertilizer respectively;
Wherein, the mass ratio of natural pond liquid, EM bacterium stoste and EM bacterial nutrient solution is 98:3:0.8.
Embodiment 4
S41, natural pond liquid is carried out isolation and identification through ultra-filtration membrane and reverse osmosis membrane, obtain concentrated natural pond liquid and water, and natural pond liquid disinfection will be concentrated;
The EM bacterium stoste point added for 2 times containing photosynthetic bacteria group, lactobacillus, yeast flora, Gram positive actinomycetes group in S42, concentrated natural pond liquid in step S41 after sterilization, add the EM bacterial nutrient solution comprising G/W, be under the condition of 15 DEG C in temperature, carry out 20 days EM bacterium to cultivate, and adopt diving reflux pump to carry out return stirring, add copper, iron, cobalt element after cultivation completes, and adopt 100 order plate-and-frame filter press to carry out solid-liquid separation, obtain solid-state and liquid biological organic fertilizer respectively;
Wherein, the mass ratio of natural pond liquid, EM bacterium stoste and EM bacterial nutrient solution is 102:5:1.2.
Embodiment 5
S51, natural pond liquid is carried out isolation and identification through ultra-filtration membrane and reverse osmosis membrane, obtain concentrated natural pond liquid and water, and natural pond liquid disinfection will be concentrated;
The EM bacterium stoste of the thread flora added for 3 times containing photosynthetic bacteria group, lactobacillus, yeast flora, Gram positive actinomycetes group, fermentation system is divided in concentrated natural pond liquid after S52, in step s 51 sterilization, add the EM bacterial nutrient solution comprising brown sugar water and G/W, be under the condition of 25 DEG C in temperature, carry out 30 days EM bacterium to cultivate, and adopt blower fan to carry out intermittent aeration stirring, selenium, boron, zinc, manganese, molybdenum, copper, iron, cobalt element is added after cultivation completes, and adopt 120 order plate-and-frame filter press to carry out solid-liquid separation, obtain solid-state and liquid biological organic fertilizer respectively;
Wherein, the mass ratio of natural pond liquid, EM bacterium stoste and EM bacterial nutrient solution is 100:4:1.
Experimental example 1EM bacteria organic fertilizer Middle nutrition detection of content of element
The EM bacteria organic fertilizer that embodiment 1-5 is provided carry out total nitrogen, total phosphorus, total potassium containing quantitative analysis, and contrast with the natural pond liquid not carrying out the cultivation of EM bacterium, its experimental result is as shown in table 1.
Table 1 different EM bacteria organic fertilizer Analysis of Nutritional
As can be seen from Table 1, the nutrient contg of EM bacteria organic fertilizer provided by the present invention is all higher than comparative example, and namely through the natural pond liquid that EM bacterium is cultivated, nutrient composition content is higher.Further, the EM bacteria organic fertilizer nutrient contg provided in embodiment 5 is the highest, is optimum embodiment.
Experimental example 2 planting experiment
Adopt the EM bacteria organic fertilizer that provides of embodiment 5 to test, and directly use with natural pond liquid and contrast.
(1) strawberry experiment, is planted
Test with throwing in Buddha town, Yangxin County, Huangshi strawberry cultivating in July, 2015, sample plot totally 10 mu, put-on method: mid-July every mu throws in EM bacteria organic fertilizer provided by the invention 1.5 tons, carry out Soil tillage, grand year seedling has been started after 15 days, topdress 0.3 ton every mu October, just have mid-December strawberry to pluck.
After employing EM bacteria organic fertilizer provided by the invention, strawberry blight does not almost have, and has positive effect to soil compaction, and the Strawberry ripening phase has done sth. in advance about 15 days, volume increase about 30%, pure in mouth feel, and strawberry fruit neither one is hollow.
(2), planting vegetable (Brassica rapa L) experiment
In September, 2015 EM bacteria organic fertilizer is rendered to (Brassica rapa L) on vegetable field to use, output volume increase 50%.
After employing EM bacteria organic fertilizer provided by the invention, vegetables is good, deposit length, will do sth. in advance harvest in 7 days than the vegetables same period.
Above description of test, not only nutrition content is high for this EM bacteria organic fertilizer, also containing multiple-microorganism, contributes to decomposing organic matter, makes nutrition very easily absorbed by crops, improve the seed output and quality of strawberry and vegetables.
In sum, the method utilizing natural pond liquid to prepare EM bacteria organic fertilizer provided by the invention, adopts the mode that EM bacterium is cultivated, the advantages such as the method has conveniently, simple, easy handling.The natural pond liquid that utilizes provided by the invention prepares EM bacteria organic fertilizer, containing abundant organic matter and a large amount of beneficial organism bacterium, has the effect of improvement soil.There is the advantages such as nutritious, yield and quality that is that be easily absorbed by plants, can improve farm crop, be the comprehensive foliage fertilizer of a kind of nutrient, can be used for all farm crop, fruit and flowers.The natural pond liquid that utilizes provided by the invention prepares EM bacteria organic fertilizer, can prevent and treat some diseases and pests of agronomic crop, can prevent eliminating aphis, red, yellow spider, rice leafhopper, planthopper, banded sclerotial blight etc.This EM bacteria organic fertilizer, there is improvement soil, strengthen photosynthesis, improve water quality, deodorizing excrement, growth promotion, the effect such as disease-resistant, antibacterial.
Although illustrate and describe the present invention with specific embodiment, however it will be appreciated that can to make when not deviating from the spirit and scope of the present invention many other change and amendment.Therefore, this means to comprise all such changes and modifications belonged in the scope of the invention in the following claims.
Claims (10)
1. utilize natural pond liquid to prepare a method for EM bacteria organic fertilizer, it is characterized in that, comprise the following steps:
Add in the liquid of natural pond by EM bacterium stoste and EM bacterial nutrient solution, natural pond liquid, after EM bacterium is cultivated, obtains biological organic fertilizer;
Wherein, the mass ratio of natural pond liquid, EM bacterium stoste and EM bacterial nutrient solution is (95-105): (2-6): (0.5-1.5).
2. the method utilizing natural pond liquid to prepare EM bacteria organic fertilizer according to claim 1, is characterized in that, specifically comprise the following steps:
1), by natural pond liquid carry out isolation and identification through ultra-filtration membrane and reverse osmosis membrane, obtain concentrated natural pond liquid and water, and natural pond liquid disinfection will be concentrated;
2), in step 1) in sterilization after concentrated natural pond liquid in add EM bacterium stoste and EM bacterial nutrient solution, carry out 7-30 days EM bacterium and cultivate and stir, add trace element after having cultivated and carry out solid-liquid separation, obtaining solid-state and liquid organic fertilizer respectively;
Wherein, the mass ratio of natural pond liquid, EM bacterium stoste and EM bacterial nutrient solution is (98-102): (3-5): (0.8-1.2).
3. the method utilizing natural pond liquid to prepare EM bacteria organic fertilizer according to claim 1 and 2, it is characterized in that, described EM bacterium stoste comprises: containing one or several the bacterium liquid in the thread flora that photosynthetic bacteria group, lactobacillus, yeast flora, Gram positive actinomycetes group and fermentation are.
4. the method utilizing natural pond liquid to prepare EM bacteria organic fertilizer according to claim 1 and 2, is characterized in that, described EM bacterial nutrient solution comprises: brown sugar water and/or G/W.
5. the method utilizing natural pond liquid to prepare EM bacteria organic fertilizer according to claim 2, is characterized in that, in step 2) in, the temperature of described cultivation is 5-25 DEG C.
6. the method utilizing natural pond liquid to prepare EM bacteria organic fertilizer according to claim 2, is characterized in that, in step 2) in, described EM bacterium stoste is divided 1-3 time and is added.
7. the method utilizing natural pond liquid to prepare EM bacteria organic fertilizer according to claim 2, is characterized in that, in step 2) in, the described one stirred in employing submersible water impeller, diving reflux pump or blower fan.
8. the method utilizing natural pond liquid to prepare EM bacteria organic fertilizer according to claim 2, is characterized in that, in step 2) in, described trace element is one or more in selenium, boron, zinc, manganese, molybdenum, copper, iron, cobalt.
9. the method utilizing natural pond liquid to prepare EM bacteria organic fertilizer according to claim 2, is characterized in that, in step 2) in, described solid-liquid separation adopts 80-120 order plate-and-frame filter press to carry out.
10. the biological organic fertilizer made of the method utilizing natural pond liquid to prepare EM bacteria organic fertilizer according to any one of claim 1-9.
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| CN109880814A (en) * | 2019-04-28 | 2019-06-14 | 中国热带农业科学院农产品加工研究所 | A method of it is extracted from pineapple bran and obtains bromelain |
| CN110372447A (en) * | 2019-08-27 | 2019-10-25 | 左会军 | A kind of nutrient solution and preparation method for the volume increase of Arid&semi-arid area ansu apricot tree |
| CN110437005A (en) * | 2019-09-05 | 2019-11-12 | 新疆农业科学院土壤肥料与农业节水研究所(新疆维吾尔自治区新型肥料研究中心) | A kind of faintly acid salt resistance alkali biological liquid fertilizer and preparation method thereof |
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