CN105495610A - Preparation method of cordyceps militaris and apple enzyme capable of improving immunity - Google Patents
Preparation method of cordyceps militaris and apple enzyme capable of improving immunity Download PDFInfo
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- 241001264174 Cordyceps militaris Species 0.000 title claims abstract description 27
- 230000036039 immunity Effects 0.000 title claims abstract description 12
- 238000002360 preparation method Methods 0.000 title claims abstract description 9
- 108090000790 Enzymes Proteins 0.000 title abstract description 6
- 102000004190 Enzymes Human genes 0.000 title abstract description 6
- 238000000855 fermentation Methods 0.000 claims abstract description 19
- 230000004151 fermentation Effects 0.000 claims abstract description 19
- 230000001954 sterilising effect Effects 0.000 claims abstract description 17
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 17
- 239000000284 extract Substances 0.000 claims abstract description 13
- 241000186660 Lactobacillus Species 0.000 claims abstract description 9
- 229940039696 lactobacillus Drugs 0.000 claims abstract description 9
- 239000007788 liquid Substances 0.000 claims description 44
- 241000894006 Bacteria Species 0.000 claims description 32
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims description 24
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 22
- 239000012530 fluid Substances 0.000 claims description 20
- 239000000706 filtrate Substances 0.000 claims description 16
- 239000001963 growth medium Substances 0.000 claims description 16
- 238000011081 inoculation Methods 0.000 claims description 16
- 239000002609 medium Substances 0.000 claims description 16
- 238000010899 nucleation Methods 0.000 claims description 16
- 235000014655 lactic acid Nutrition 0.000 claims description 12
- 239000004310 lactic acid Substances 0.000 claims description 12
- 238000000034 method Methods 0.000 claims description 10
- 241000196324 Embryophyta Species 0.000 claims description 8
- 241001052560 Thallis Species 0.000 claims description 8
- 229940041514 candida albicans extract Drugs 0.000 claims description 8
- 239000002054 inoculum Substances 0.000 claims description 8
- 238000002156 mixing Methods 0.000 claims description 8
- 235000015097 nutrients Nutrition 0.000 claims description 8
- 239000000843 powder Substances 0.000 claims description 8
- 210000002966 serum Anatomy 0.000 claims description 8
- 239000012138 yeast extract Substances 0.000 claims description 8
- 229920001817 Agar Polymers 0.000 claims description 4
- 241000194108 Bacillus licheniformis Species 0.000 claims description 4
- 241000186000 Bifidobacterium Species 0.000 claims description 4
- 235000010149 Brassica rapa subsp chinensis Nutrition 0.000 claims description 4
- 244000221633 Brassica rapa subsp chinensis Species 0.000 claims description 4
- 244000241257 Cucumis melo Species 0.000 claims description 4
- 235000015510 Cucumis melo subsp melo Nutrition 0.000 claims description 4
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 4
- 239000001888 Peptone Substances 0.000 claims description 4
- 108010080698 Peptones Proteins 0.000 claims description 4
- 241000220324 Pyrus Species 0.000 claims description 4
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 4
- 241000235342 Saccharomycetes Species 0.000 claims description 4
- 241000194020 Streptococcus thermophilus Species 0.000 claims description 4
- 235000009754 Vitis X bourquina Nutrition 0.000 claims description 4
- 235000012333 Vitis X labruscana Nutrition 0.000 claims description 4
- 240000006365 Vitis vinifera Species 0.000 claims description 4
- 235000014787 Vitis vinifera Nutrition 0.000 claims description 4
- FJJCIZWZNKZHII-UHFFFAOYSA-N [4,6-bis(cyanoamino)-1,3,5-triazin-2-yl]cyanamide Chemical compound N#CNC1=NC(NC#N)=NC(NC#N)=N1 FJJCIZWZNKZHII-UHFFFAOYSA-N 0.000 claims description 4
- 239000008272 agar Substances 0.000 claims description 4
- 235000015278 beef Nutrition 0.000 claims description 4
- AIUDWMLXCFRVDR-UHFFFAOYSA-N dimethyl 2-(3-ethyl-3-methylpentyl)propanedioate Chemical compound CCC(C)(CC)CCC(C(=O)OC)C(=O)OC AIUDWMLXCFRVDR-UHFFFAOYSA-N 0.000 claims description 4
- 239000008103 glucose Substances 0.000 claims description 4
- 230000008676 import Effects 0.000 claims description 4
- 238000002386 leaching Methods 0.000 claims description 4
- 238000012856 packing Methods 0.000 claims description 4
- 235000021017 pears Nutrition 0.000 claims description 4
- 235000019319 peptone Nutrition 0.000 claims description 4
- 239000006228 supernatant Substances 0.000 claims description 4
- 239000000243 solution Substances 0.000 abstract 3
- 239000011550 stock solution Substances 0.000 abstract 2
- 241000235003 Saccharomycopsis Species 0.000 abstract 1
- 239000000047 product Substances 0.000 description 9
- 210000000952 spleen Anatomy 0.000 description 7
- 210000001541 thymus gland Anatomy 0.000 description 6
- 241000233866 Fungi Species 0.000 description 5
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- 241001248610 Ophiocordyceps sinensis Species 0.000 description 3
- 210000004072 lung Anatomy 0.000 description 3
- 238000012423 maintenance Methods 0.000 description 3
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- 238000003756 stirring Methods 0.000 description 3
- 241000699670 Mus sp. Species 0.000 description 2
- 210000003734 kidney Anatomy 0.000 description 2
- 206010011224 Cough Diseases 0.000 description 1
- 101100256850 Drosophila melanogaster EndoA gene Proteins 0.000 description 1
- 238000012449 Kunming mouse Methods 0.000 description 1
- 206010036790 Productive cough Diseases 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000002929 anti-fatigue Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000002210 biocatalytic effect Effects 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 235000008429 bread Nutrition 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 230000007969 cellular immunity Effects 0.000 description 1
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- 238000002474 experimental method Methods 0.000 description 1
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- 238000012986 modification Methods 0.000 description 1
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- 235000016709 nutrition Nutrition 0.000 description 1
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- 210000003802 sputum Anatomy 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000002992 thymic effect Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/21—Streptococcus, lactococcus
- A23V2400/249—Thermophilus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/51—Bifidobacterium
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- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention adopts a technical solution, namely a preparation method of a cordyceps militaris and apple enzyme capable of improving immunity. The preparation method comprises steps as follows: step 1: a saccharomycopsis solution is prepared; step 2: a lactobacillus solution is prepared; step 3: a fermentation broth is cultured; step 4: the fermentation broth is subjected to centrifugal treatment and an enzyme stock solution is obtained; step 5: a cordyceps militaris extract and the enzyme stock solution obtained in the step 4 are mixed in the mass ratio being 94:1,000 and maintained for 30-50 min at the temperature of 115 DEG C for high-temperature sterilization, and subpackaging is performed.
Description
Technical field
The invention belongs to biologic product technology field, particularly relate to a kind of preparation method improving the Cordyceps militaris apple ferment of immunity.
Background technology
Ferment is also called " enzyme ", is the polymer substance with biocatalytic Activity.Nearly all cellular activity process all needs the participation of ferment.Modern society, along with the quickening of people's rhythm of life and the aggravation of environmental pollution, the content of people's body endo enzyme reduces day by day, and then reduces body immunity and metabolic rate, and the probability making people ill increases.At present, the ferment content in daily bread is too low, cannot meet the normal need of people.In order to meet modern to diversity, the balance of nutritional need be easy to absorbability, the production of efficient ferment product is extremely urgent.
Summary of the invention
The defect that the present invention exists to overcome prior art, the object of this invention is to provide a kind of preparation method improving the Cordyceps militaris apple ferment of immunity.
The technology used in the present invention solution is a kind of preparation method improving the Cordyceps militaris apple ferment of immunity, and required step is as follows:
Step 1: prepare barms liquid:
Plant flow container and expand culture medium: YPD fluid nutrient medium, its mass percent is 1% yeast extract, 2% peptone, 2% glucose, 2% agar, and all the other are water, and YPD fluid nutrient medium is put into 0.5M
3in seeding tank, the 30-50 minute high-temperature sterilization of 115 DEG C; Under aseptic technique, 1.5 kilograms of saccharomycete dry bacterium powders are dissolved in the sterilized water of 3 times, form bacterium liquid, described bacterium liquid is loaded serum bottle and is inoculated into 0.5M
3seeding tank, passes into aseptic compressed air at 28-30 DEG C, and throughput is 0.2-0.3 cubic meters per minute, cultivates 20-30h, until thalli growth to stopping during logarithmic phase latter stage cultivating, repeats the kind liquid that above-mentioned steps can obtain the yeast liquid of required quality;
Step 2: prepare lactobacillus inoculation liquid:
Plant flow container and expand culture medium: enrichment medium of wort, its mass percent is 10% brewer's wort, 0.5% soy peptone, 0.5% yeast extract, 1% beef extract, 0.2%K
2hPO
4,all the other are water, and enrichment medium of wort is put into 0.5M
3in seeding tank, the 30-50 minute high-temperature sterilization of 115 DEG C; Be dissolved in by the dry bacterium powder of 1.5 kilograms of lactic acid bacterias in the sterilized water of 3 times under aseptic technique, bacterium liquid loads serum bottle and is inoculated into 0.5M
3seeding tank, 37 DEG C of constant temperature quiescent culture 24-36h, until thalli growth to stopping during logarithmic phase latter stage cultivating, can repeat the kind liquid that above-mentioned steps can obtain required lactic acid bacteria;
Step 3: cultivation and fermentation liquid:
Be first the apple of 140:20:20:18:2:1000 by mass ratio: pears: "Hami" melon: pakchoi: grape: leaching filtrate was smashed in water mixing, culture medium is made with described filtrate, high-temperature sterilization 30-50 minute at 115 DEG C, described culture medium is added in fermentation tank, then be the saccharomycetic kind of liquid that the inoculum concentration inoculation step 1 of the 5%-10% of described filtrate obtains by quality, 48h is cultivated at the temperature of 28 DEG C, the volume of described fermentation tank is V cubic meter, throughput is (0.4-0.6) V cubic meters per minute, speed of agitator 100-120rpm/min; Then be the lactobacillus inoculation liquid that the inoculum concentration inoculation step 2 of the 5%-10% of described filtrate obtains by quality, at the temperature of 37 DEG C, continue to cultivate 5-7d (quiescent culture, without stirring, allows tank maintenance malleation in case microbiological contamination), to fermentation ends, obtaining zymotic fluid;
Step 4: zymotic fluid centrifugal treating is obtained ferment stoste:
Zymotic fluid step 3 obtained imports centrifuge, and through the centrifugal 10min of 6000rpm, gained supernatant is ferment stoste;
Step 5:
By mass ratio be: Cordyceps militaris extract: the ferment stoste mixing that the Cordyceps militaris extract of ferment stoste=94:1000 and step 4 obtain, maintains 30-50 minute, carry out high-temperature sterilization, then carry out packing under 115 DEG C of environment.
Lactic acid bacteria in described step 2 is 1.3 kilograms of Bifidobacteriums, 0.1 kilogram of bacillus licheniformis, 0.1 kilogram of streptococcus thermophilus.
Compared with prior art, the beneficial effect that the present invention has is:
Cordyceps militaris has the active component similar with Cordyceps sinensis and pharmacological action, is called as " Cordyceps sinensis " that can manually cultivate.2009, Cordyceps militaris was approved for new resource food, and its price is far below Cordyceps sinensis, was thus more and more subject to the favor of consumers in general.The traditional Chinese medical science is thought, Cordyceps militaris enters lung kidney two warp, and both tonifying lung was cloudy, and kidney-replenishing again, function cures mainly as invigorating the lung and the kidney, relieving cough and reducing sputum.Modern medicine study its there is cellular immunity and Humoral Immunological Regulation Roles, also there is antifatigue effect.Therefore, be the product of primary raw material if can develop with Cordyceps militaris, further developing of Cordyceps militaris industry can not only be promoted, more can bring happiness for the health of broad masses of the people.Although the product that on market, existing Cordyceps militaris is relevant, market scale is little, and added value is low, and effect is indefinite, needs badly and develops cordyceps militaris series products that is efficient, high added value.
Both, in conjunction with the features of ferment and Cordyceps militaris, are carried out mixed culture fermentation or composite, then are equipped with other active component, develop Cordyceps militaris ferment product innovatively by the present invention.Effect of this invention set ferment and Cordyceps militaris, has the effect improving immunity.
Show by experiment, Chinese caterpillar fungus ferment group Thymus and Spleen index is all higher than Normal group, and difference has conspicuousness (p < 0.01), illustrates that the immunity of product group promotes Be very effective.
Detailed description of the invention
Embodiment 1:
Step 1: prepare barms liquid:
Plant flow container and expand culture medium: YPD fluid nutrient medium, its mass percent is 1% yeast extract, 2% peptone, 2% glucose, 2% agar, and all the other are water, and YPD fluid nutrient medium is put into 0.5M
3in seeding tank, 30 minutes high-temperature sterilizations of 115 DEG C; Under aseptic technique, 1.5 kilograms of saccharomycete dry bacterium powders are dissolved in the sterilized water of 3 times, form bacterium liquid, described bacterium liquid is loaded serum bottle and is inoculated into 0.5M
3seeding tank, passes into aseptic compressed air at 28 DEG C, and throughput is 0.2 cubic meters per minute, cultivates 20h, until thalli growth to stopping during logarithmic phase latter stage cultivating, repeats the kind liquid that above-mentioned steps can obtain the yeast liquid of required quality;
Step 2: prepare lactobacillus inoculation liquid:
Plant flow container and expand culture medium: enrichment medium of wort, its mass percent is 10% brewer's wort, 0.5% soy peptone, 0.5% yeast extract, 1% beef extract, 0.2%K
2hPO
4,all the other are water, and enrichment medium of wort is put into 0.5M
3in seeding tank, 30 minutes high-temperature sterilizations of 115 DEG C; Be dissolved in by the dry bacterium powder of 1.5 kilograms of lactic acid bacterias in the sterilized water of 3 times under aseptic technique, bacterium liquid loads serum bottle and is inoculated into 0.5M
3seeding tank, 37 DEG C of constant temperature quiescent culture 24h, until thalli growth to stopping during logarithmic phase latter stage cultivating, can repeat the kind liquid that above-mentioned steps can obtain required lactic acid bacteria; Lactic acid bacteria in described step 2 is 1.3 kilograms of Bifidobacteriums, 0.1 kilogram of bacillus licheniformis, 0.1 kilogram of streptococcus thermophilus.
Step 3: cultivation and fermentation liquid:
Be first the apple of 140:20:20:18:2:1000 by mass ratio: pears: "Hami" melon: pakchoi: grape: leaching filtrate was smashed in water mixing, culture medium is made with described filtrate, high-temperature sterilization 30 minutes at 115 DEG C, described culture medium is added in fermentation tank, then the saccharomycetic kind of liquid that the inoculum concentration inoculation step 1 being 5% of described filtrate by quality obtains, 48h is cultivated at the temperature of 28 DEG C, the volume of described fermentation tank is V cubic meter, throughput is 0.4V cubic meters per minute, speed of agitator 100rpm/min; Then the lactobacillus inoculation liquid that the inoculum concentration inoculation step 2 being 5% of described filtrate by quality obtains, continues to cultivate 5d (quiescent culture, without stirring, allows tank maintenance malleation in case microbiological contamination) and, to fermentation ends, obtains zymotic fluid at the temperature of 37 DEG C;
Step 4: zymotic fluid centrifugal treating is obtained ferment stoste:
Zymotic fluid step 3 obtained imports centrifuge, and through the centrifugal 10min of 6000rpm, gained supernatant is ferment stoste;
Step 5:
By mass ratio be: Cordyceps militaris extract: the ferment stoste mixing that the Cordyceps militaris extract of ferment stoste=94:1000 and step 4 obtain, maintains 30 minutes, carry out high-temperature sterilization, then carry out packing under 115 DEG C of environment.
Embodiment 2:
Step 1: prepare barms liquid:
Plant flow container and expand culture medium: YPD fluid nutrient medium, its mass percent is 1% yeast extract, 2% peptone, 2% glucose, 2% agar, and all the other are water, and YPD fluid nutrient medium is put into 0.5M
3in seeding tank, 50 minutes high-temperature sterilizations of 115 DEG C; Under aseptic technique, 1.5 kilograms of saccharomycete dry bacterium powders are dissolved in the sterilized water of 3 times, form bacterium liquid, described bacterium liquid is loaded serum bottle and is inoculated into 0.5M
3seeding tank, passes into aseptic compressed air at 30 DEG C, and throughput is 0.3 cubic meters per minute, cultivates 30h, until thalli growth to stopping during logarithmic phase latter stage cultivating, repeats the kind liquid that above-mentioned steps can obtain the yeast liquid of required quality;
Step 2: prepare lactobacillus inoculation liquid:
Plant flow container and expand culture medium: enrichment medium of wort, its mass percent is 10% brewer's wort, 0.5% soy peptone, 0.5% yeast extract, 1% beef extract, 0.2%K
2hPO
4,all the other are water, and enrichment medium of wort is put into 0.5M
3in seeding tank, 50 minutes high-temperature sterilizations of 115 DEG C; Be dissolved in by the dry bacterium powder of 1.5 kilograms of lactic acid bacterias in the sterilized water of 3 times under aseptic technique, bacterium liquid loads serum bottle and is inoculated into 0.5M
3seeding tank, 37 DEG C of constant temperature quiescent culture 36h, until thalli growth to stopping during logarithmic phase latter stage cultivating, can repeat the kind liquid that above-mentioned steps can obtain required lactic acid bacteria; Lactic acid bacteria in described step 2 is 1.3 kilograms of Bifidobacteriums, 0.1 kilogram of bacillus licheniformis, 0.1 kilogram of streptococcus thermophilus.
Step 3: cultivation and fermentation liquid:
Be first the apple of 140:20:20:18:2:1000 by mass ratio: pears: "Hami" melon: pakchoi: grape: leaching filtrate was smashed in water mixing, culture medium is made with described filtrate, high-temperature sterilization 30-50 minute at 115 DEG C, described culture medium is added in fermentation tank, then the saccharomycetic kind of liquid that the inoculum concentration inoculation step 1 being 10% of described filtrate by quality obtains, 48h is cultivated at the temperature of 28 DEG C, the volume of described fermentation tank is V cubic meter, throughput is 0.6V cubic meters per minute, speed of agitator 120rpm/min; Then be the lactobacillus inoculation liquid that the inoculum concentration inoculation step 2 of the 5%-10% of described filtrate obtains by quality, at the temperature of 37 DEG C, continue to cultivate 7d (quiescent culture, without stirring, allows tank maintenance malleation in case microbiological contamination), to fermentation ends, obtaining zymotic fluid;
Step 4: zymotic fluid centrifugal treating is obtained ferment stoste:
Zymotic fluid step 3 obtained imports centrifuge, and through the centrifugal 10min of 6000rpm, gained supernatant is ferment stoste;
Step 5:
By mass ratio be: Cordyceps militaris extract: the ferment stoste mixing that the Cordyceps militaris extract of ferment stoste=94:1000 and step 4 obtain, maintains 50 minutes, carry out high-temperature sterilization, then carry out packing under 115 DEG C of environment.
Improve immunity
Adopt feeding method to feed kunming mice, be divided at random: Normal group (pure water group), embodiment product group (Chinese caterpillar fungus ferment group), often organize 10.Chinese caterpillar fungus ferment group gives Cordyceps militaris ferment tested material with the volume of 0.1mL/20g every day, and negative control group gives equal-volume pure water, after continuous 30d, weigh, eye socket is got blood and is put to death, and divides and gets thymus gland and spleen, weigh, calculate thymus index and index and spleen index respectively by following formula:
Thymus index=thymic weight (mg)/mice weights (g)
Index and spleen index=spleen weight (mg)/mice weights (g)
The results are shown in Table, Chinese caterpillar fungus ferment group Thymus and Spleen index is all higher than Normal group, and difference has conspicuousness (p < 0.01), illustrates that the immunity of product group promotes Be very effective.
| Grouping | Thymus index | Index and spleen index |
| Normal group | 3.15±0.29 | 3.44±0.75 |
| Chinese caterpillar fungus ferment group | 3.33±0.37 | 4.10±0.69 |
More than show and describe general principle of the present invention, principal character and advantage of the present invention.The technical staff of the industry should understand; the present invention is not restricted to the described embodiments; what describe in above-described embodiment and description just illustrates principle of the present invention; the present invention also has various changes and modifications without departing from the spirit and scope of the present invention, and these changes and improvements all fall in the claimed scope of the invention.Application claims protection domain is defined by appending claims and equivalent thereof.
Claims (2)
1. improve a preparation method for the Cordyceps militaris apple ferment of immunity, it is characterized in that: required step is as follows:
Step 1: prepare barms liquid:
Plant flow container and expand culture medium: YPD fluid nutrient medium, its mass percent is 1% yeast extract, 2% peptone, 2% glucose, 2% agar, and all the other are water, and YPD fluid nutrient medium is put into 0.5M
3in seeding tank, the 30-50 minute high-temperature sterilization of 115 DEG C; Under aseptic technique, 1.5 kilograms of saccharomycete dry bacterium powders are dissolved in the sterilized water of 3 times, form bacterium liquid, described bacterium liquid is loaded serum bottle and is inoculated into 0.5M
3seeding tank, passes into aseptic compressed air at 28-30 DEG C, and throughput is 0.2-0.3 cubic meters per minute, cultivates 20-30h, until thalli growth to stopping during logarithmic phase latter stage cultivating, repeats the kind liquid that above-mentioned steps can obtain the yeast liquid of required quality;
Step 2: prepare lactobacillus inoculation liquid:
Plant flow container and expand culture medium: enrichment medium of wort, its mass percent is 10% brewer's wort, 0.5% soy peptone, 0.5% yeast extract, 1% beef extract, 0.2%K
2hPO
4,all the other are water, and enrichment medium of wort is put into 0.5M
3in seeding tank, the 30-50 minute high-temperature sterilization of 115 DEG C; Be dissolved in by the dry bacterium powder of 1.5 kilograms of lactic acid bacterias in the sterilized water of 3 times under aseptic technique, bacterium liquid loads serum bottle and is inoculated into 0.5M
3seeding tank, 37 DEG C of constant temperature quiescent culture 24-36h, until thalli growth to stopping during logarithmic phase latter stage cultivating, can repeat the kind liquid that above-mentioned steps can obtain required lactic acid bacteria;
Step 3: cultivation and fermentation liquid:
Be first the apple of 140:20:20:18:2:1000 by mass ratio: pears: "Hami" melon: pakchoi: grape: leaching filtrate was smashed in water mixing, culture medium is made with described filtrate, high-temperature sterilization 30-50 minute at 115 DEG C, described culture medium is added in fermentation tank, then be the saccharomycetic kind of liquid that the inoculum concentration inoculation step 1 of the 5%-10% of described filtrate obtains by quality, 48h is cultivated at the temperature of 28 DEG C, the volume of described fermentation tank is V cubic meter, throughput is (0.4-0.6) V cubic meters per minute, speed of agitator 100-120rpm/min; Then be the lactobacillus inoculation liquid that the inoculum concentration inoculation step 2 of the 5%-10% of described filtrate obtains by quality, continue to cultivate 5-7d to fermentation ends at the temperature of 37 DEG C, obtain zymotic fluid;
Step 4: zymotic fluid centrifugal treating is obtained ferment stoste:
Zymotic fluid step 3 obtained imports centrifuge, and through the centrifugal 10min of 6000rpm, gained supernatant is ferment stoste;
Step 5:
By mass ratio be: Cordyceps militaris extract: the ferment stoste mixing that the Cordyceps militaris extract of ferment stoste=94:1000 and step 4 obtain, maintains 30-50 minute, carry out high-temperature sterilization, then carry out packing under 115 DEG C of environment.
2. preparation method according to claim 1, is characterized in that: the lactic acid bacteria in described step 2 is 1.3 kilograms of Bifidobacteriums, 0.1 kilogram of bacillus licheniformis, 0.1 kilogram of streptococcus thermophilus.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111528427A (en) * | 2020-05-09 | 2020-08-14 | 青岛国海生物制药有限公司 | Laiyang pear enzyme paste added with cordyceps militaris and preparation method of Laiyang pear enzyme paste |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| KR20140057838A (en) * | 2012-11-05 | 2014-05-14 | 노길형 | Fermented foods process and production method |
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