CN105361180A - Preparation method of cordyceps militaris pomegranate enzyme with effect of beautifying features - Google Patents
Preparation method of cordyceps militaris pomegranate enzyme with effect of beautifying features Download PDFInfo
- Publication number
- CN105361180A CN105361180A CN201510865258.2A CN201510865258A CN105361180A CN 105361180 A CN105361180 A CN 105361180A CN 201510865258 A CN201510865258 A CN 201510865258A CN 105361180 A CN105361180 A CN 105361180A
- Authority
- CN
- China
- Prior art keywords
- liquid
- cordyceps militaris
- ferment
- minute
- pomegranate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 241001264174 Cordyceps militaris Species 0.000 title claims abstract description 29
- 235000014360 Punica granatum Nutrition 0.000 title claims abstract description 11
- 238000002360 preparation method Methods 0.000 title claims abstract description 8
- 241000219991 Lythraceae Species 0.000 title abstract description 9
- 108090000790 Enzymes Proteins 0.000 title abstract description 7
- 102000004190 Enzymes Human genes 0.000 title abstract description 7
- 230000000694 effects Effects 0.000 title abstract description 7
- 241000894006 Bacteria Species 0.000 claims abstract description 33
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims abstract description 26
- 238000000855 fermentation Methods 0.000 claims abstract description 18
- 230000004151 fermentation Effects 0.000 claims abstract description 18
- 230000001954 sterilising effect Effects 0.000 claims abstract description 17
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 17
- 239000000284 extract Substances 0.000 claims abstract description 14
- 235000014655 lactic acid Nutrition 0.000 claims abstract description 13
- 239000004310 lactic acid Substances 0.000 claims abstract description 13
- 238000000034 method Methods 0.000 claims abstract description 10
- 238000002156 mixing Methods 0.000 claims abstract description 9
- 239000007788 liquid Substances 0.000 claims description 44
- 239000012530 fluid Substances 0.000 claims description 20
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 20
- 239000000706 filtrate Substances 0.000 claims description 16
- 239000001963 growth medium Substances 0.000 claims description 16
- 238000011081 inoculation Methods 0.000 claims description 16
- 239000002609 medium Substances 0.000 claims description 16
- 238000010899 nucleation Methods 0.000 claims description 16
- 241000196324 Embryophyta Species 0.000 claims description 8
- 241000186660 Lactobacillus Species 0.000 claims description 8
- 241001052560 Thallis Species 0.000 claims description 8
- 229940041514 candida albicans extract Drugs 0.000 claims description 8
- 239000002054 inoculum Substances 0.000 claims description 8
- 229940039696 lactobacillus Drugs 0.000 claims description 8
- 235000015097 nutrients Nutrition 0.000 claims description 8
- 239000000843 powder Substances 0.000 claims description 8
- 210000002966 serum Anatomy 0.000 claims description 8
- 239000012138 yeast extract Substances 0.000 claims description 8
- 239000002537 cosmetic Substances 0.000 claims description 7
- 229920001817 Agar Polymers 0.000 claims description 4
- 244000144730 Amygdalus persica Species 0.000 claims description 4
- 241000186000 Bifidobacterium Species 0.000 claims description 4
- 244000241257 Cucumis melo Species 0.000 claims description 4
- 235000015510 Cucumis melo subsp melo Nutrition 0.000 claims description 4
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 4
- 235000007688 Lycopersicon esculentum Nutrition 0.000 claims description 4
- 239000001888 Peptone Substances 0.000 claims description 4
- 108010080698 Peptones Proteins 0.000 claims description 4
- 235000006040 Prunus persica var persica Nutrition 0.000 claims description 4
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 4
- 241000235342 Saccharomycetes Species 0.000 claims description 4
- 240000003768 Solanum lycopersicum Species 0.000 claims description 4
- 241000194020 Streptococcus thermophilus Species 0.000 claims description 4
- 235000009754 Vitis X bourquina Nutrition 0.000 claims description 4
- 235000012333 Vitis X labruscana Nutrition 0.000 claims description 4
- 240000006365 Vitis vinifera Species 0.000 claims description 4
- 235000014787 Vitis vinifera Nutrition 0.000 claims description 4
- FJJCIZWZNKZHII-UHFFFAOYSA-N [4,6-bis(cyanoamino)-1,3,5-triazin-2-yl]cyanamide Chemical compound N#CNC1=NC(NC#N)=NC(NC#N)=N1 FJJCIZWZNKZHII-UHFFFAOYSA-N 0.000 claims description 4
- 239000008272 agar Substances 0.000 claims description 4
- 235000015278 beef Nutrition 0.000 claims description 4
- AIUDWMLXCFRVDR-UHFFFAOYSA-N dimethyl 2-(3-ethyl-3-methylpentyl)propanedioate Chemical compound CCC(C)(CC)CCC(C(=O)OC)C(=O)OC AIUDWMLXCFRVDR-UHFFFAOYSA-N 0.000 claims description 4
- 239000008103 glucose Substances 0.000 claims description 4
- 230000008676 import Effects 0.000 claims description 4
- 238000002386 leaching Methods 0.000 claims description 4
- 238000012856 packing Methods 0.000 claims description 4
- 235000019319 peptone Nutrition 0.000 claims description 4
- 239000006228 supernatant Substances 0.000 claims description 4
- 244000294611 Punica granatum Species 0.000 claims 2
- 239000000243 solution Substances 0.000 abstract 3
- 239000011550 stock solution Substances 0.000 abstract 3
- 239000000203 mixture Substances 0.000 abstract 2
- 238000005119 centrifugation Methods 0.000 abstract 1
- 239000000047 product Substances 0.000 description 6
- 238000011156 evaluation Methods 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- 241001248610 Ophiocordyceps sinensis Species 0.000 description 3
- 210000004072 lung Anatomy 0.000 description 3
- 238000012423 maintenance Methods 0.000 description 3
- 238000011169 microbiological contamination Methods 0.000 description 3
- 238000003756 stirring Methods 0.000 description 3
- 206010015150 Erythema Diseases 0.000 description 2
- 239000001913 cellulose Substances 0.000 description 2
- 229920002678 cellulose Polymers 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
- 231100000321 erythema Toxicity 0.000 description 2
- 230000003203 everyday effect Effects 0.000 description 2
- 210000003734 kidney Anatomy 0.000 description 2
- 239000011148 porous material Substances 0.000 description 2
- 230000037394 skin elasticity Effects 0.000 description 2
- 206010011224 Cough Diseases 0.000 description 1
- 101100256850 Drosophila melanogaster EndoA gene Proteins 0.000 description 1
- 206010036790 Productive cough Diseases 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000002929 anti-fatigue Effects 0.000 description 1
- 230000003796 beauty Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000002210 biocatalytic effect Effects 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 235000008429 bread Nutrition 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 230000007969 cellular immunity Effects 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 230000002354 daily effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 230000037323 metabolic rate Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000033764 rhythmic process Effects 0.000 description 1
- 208000024794 sputum Diseases 0.000 description 1
- 210000003802 sputum Anatomy 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Tropical Medicine & Parasitology (AREA)
- Cosmetics (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The technical solution adopted by the invention lies in that the invention discloses a preparation method of a cordyceps militaris pomegranate enzyme with the effect of beautifying the features. The making method comprises the following steps of step 1, preparing a microzyme strain solution; step 2, preparing a lactic acid bacteria strain solution; step 3, performing fermentation culture; step 4, performing centrifugation treatment so as to obtain an enzyme stock solution; and step 5, mixing a cordyceps militaris extract in the mass ratio of the cordyceps militaris extract to the enzyme stock solution being 250: 1000 with the enzyme stock solution obtained in the step 4 so as to obtain a mixture, maintaining the mixture for 30-50 minutes at the environment of 115 DEG C, performing high-temperature sterilization, and then performing subpackaging.
Description
Technical field
The invention belongs to biologic product technology field, particularly relate to a kind of preparation method having the Cordyceps militaris pomegranate ferment of cosmetic result.
Background technology
Ferment is also called " enzyme ", is the polymer substance with biocatalytic Activity.Nearly all cellular activity process all needs the participation of ferment.Modern society, along with the quickening of people's rhythm of life and the aggravation of environmental pollution, the content of people's body endo enzyme reduces day by day, and then reduces body immunity and metabolic rate, and the probability making people ill increases.At present, the ferment content in daily bread is too low, cannot meet the normal need of people.In order to meet modern to diversity, the balance of nutritional need be easy to absorbability, the production of efficient ferment product is extremely urgent.
Summary of the invention
The defect that the present invention exists to overcome prior art, the object of this invention is to provide a kind of preparation method having the Cordyceps militaris pomegranate ferment of cosmetic result.
The technology used in the present invention solution is a kind of preparation method having the Cordyceps militaris pomegranate ferment of cosmetic result, and required step is as follows:
Step 1: prepare barms liquid:
Plant flow container and expand culture medium: YPD fluid nutrient medium, its mass percent is 1% yeast extract, 2% peptone, 2% glucose, 2% agar, and all the other are water, and YPD fluid nutrient medium is put into 0.5M
3in seeding tank, the 30-50 minute high-temperature sterilization of 115 DEG C; Under aseptic technique, 1.5 kilograms of saccharomycete dry bacterium powders are dissolved in the sterilized water of 3 times, form bacterium liquid, described bacterium liquid is loaded serum bottle and is inoculated into 0.5M
3seeding tank, passes into aseptic compressed air at 28-30 DEG C, and throughput is 0.2-0.3 cubic meters per minute, cultivates 20-30h, until thalli growth to stopping during logarithmic phase latter stage cultivating, repeats the kind liquid that above-mentioned steps can obtain the yeast liquid of required quality;
Step 2: prepare lactobacillus inoculation liquid:
Plant flow container and expand culture medium: enrichment medium of wort, its mass percent is 10% brewer's wort, 0.5% soy peptone, 0.5% yeast extract, 1% beef extract, 0.2%K
2hPO
4,all the other are water, and enrichment medium of wort is put into 0.5M
3in seeding tank, the 30-50 minute high-temperature sterilization of 115 DEG C; Be dissolved in by the dry bacterium powder of 1.5 kilograms of lactic acid bacterias in the sterilized water of 3 times under aseptic technique, bacterium liquid loads serum bottle and is inoculated into 0.5M
3seeding tank, 37 DEG C of constant temperature quiescent culture 24-36h, until thalli growth to stopping during logarithmic phase latter stage cultivating, can repeat the kind liquid that above-mentioned steps can obtain required lactic acid bacteria;
Step 3: cultivation and fermentation liquid:
Be first the pomegranate of 145:15:15:15:10:1000 by mass ratio: tomato: "Hami" melon: peach: grape: leaching filtrate was smashed in water mixing, culture medium is made with described filtrate, high-temperature sterilization 30-50 minute at 115 DEG C, described culture medium is added in fermentation tank, then be the saccharomycetic kind of liquid that the inoculum concentration inoculation step 1 of the 5%-10% of described filtrate obtains by quality, 48h is cultivated at the temperature of 28 DEG C, the volume of described fermentation tank is V cubic meter, throughput is (0.4-0.6) V cubic meters per minute, speed of agitator 100-120rpm/min; Then be the lactobacillus inoculation liquid that the inoculum concentration inoculation step 2 of the 5%-10% of described filtrate obtains by quality, at the temperature of 37 DEG C, continue to cultivate 5-7d (quiescent culture, without stirring, allows tank maintenance malleation in case microbiological contamination), to fermentation ends, obtaining zymotic fluid;
Step 4: zymotic fluid centrifugal treating is obtained ferment stoste:
Zymotic fluid step 3 obtained imports centrifuge, and through the centrifugal 10min of 6000rpm, gained supernatant is ferment stoste;
Step 5:
By mass ratio be: Cordyceps militaris extract: the ferment stoste mixing that the Cordyceps militaris extract of ferment stoste=250:1000 and step 4 obtain, maintains 30-50 minute, carry out high-temperature sterilization, then carry out packing under 115 DEG C of environment.
Lactic acid bacteria in described step 2 is 1 kilogram of Bifidobacterium, 0.5 kilogram of streptococcus thermophilus.
Compared with prior art, the beneficial effect that the present invention has is:
Cordyceps militaris has the active component similar with Cordyceps sinensis and pharmacological action, is called as " Cordyceps sinensis " that can manually cultivate.2009, Cordyceps militaris was approved for new resource food, and its price is far below Cordyceps sinensis, was thus more and more subject to the favor of consumers in general.The traditional Chinese medical science is thought, Cordyceps militaris enters lung kidney two warp, and both tonifying lung was cloudy, and kidney-replenishing again, function cures mainly as invigorating the lung and the kidney, relieving cough and reducing sputum.Modern medicine study its there is cellular immunity and Humoral Immunological Regulation Roles, also there is antifatigue effect.Therefore, be the product of primary raw material if can develop with Cordyceps militaris, further developing of Cordyceps militaris industry can not only be promoted, more can bring happiness for the health of broad masses of the people.Although the product that on market, existing Cordyceps militaris is relevant, market scale is little, and added value is low, and effect is indefinite, needs badly and develops cordyceps militaris series products that is efficient, high added value.
Both, in conjunction with the features of ferment and Cordyceps militaris, are carried out mixed culture fermentation or composite, then are equipped with other active component, develop Cordyceps militaris ferment product innovatively by the present invention.Effect of this invention set ferment and Cordyceps militaris, has the effect optimizing metabolic process, beauty treatment.
The evaluation and test volunteer that participation Cordyceps militaris ferment takes cosmetic result has 30 people, and sooner or later respectively taking 1 time, each 100-200ml for each person every day, 15 days is an evaluation and test cycle.Compare before and after taking, the average color cellulose content decline 15-30% of 30 people, skin elasticity increases 20-30%, and moisture content of skin increases 5-10%, and pore dia reduces 10-20%, and erythema reduces 10-20%.
Detailed description of the invention
Embodiment 1:
Step 1: prepare barms liquid:
Plant flow container and expand culture medium: YPD fluid nutrient medium, its mass percent is 1% yeast extract, 2% peptone, 2% glucose, 2% agar, and all the other are water, and YPD fluid nutrient medium is put into 0.5M
3in seeding tank, 30 minutes high-temperature sterilizations of 115 DEG C; Under aseptic technique, 1.5 kilograms of saccharomycete dry bacterium powders are dissolved in the sterilized water of 3 times, form bacterium liquid, described bacterium liquid is loaded serum bottle and is inoculated into 0.5M
3seeding tank, passes into aseptic compressed air at 28 DEG C, and throughput is 0.2 cubic meters per minute, cultivates 20h, until thalli growth to stopping during logarithmic phase latter stage cultivating, repeats the kind liquid that above-mentioned steps can obtain the yeast liquid of required quality;
Step 2: prepare lactobacillus inoculation liquid:
Plant flow container and expand culture medium: enrichment medium of wort, its mass percent is 10% brewer's wort, 0.5% soy peptone, 0.5% yeast extract, 1% beef extract, 0.2%K
2hPO
4,all the other are water, and enrichment medium of wort is put into 0.5M
3in seeding tank, 30 minutes high-temperature sterilizations of 115 DEG C; Be dissolved in by the dry bacterium powder of 1.5 kilograms of lactic acid bacterias in the sterilized water of 3 times under aseptic technique, bacterium liquid loads serum bottle and is inoculated into 0.5M
3seeding tank, 37 DEG C of constant temperature quiescent culture 24h, until thalli growth to stopping during logarithmic phase latter stage cultivating, can repeat the kind liquid that above-mentioned steps can obtain required lactic acid bacteria; Lactic acid bacteria in described step 2 is 1 kilogram of Bifidobacterium, 0.5 kilogram of streptococcus thermophilus.
Step 3: cultivation and fermentation liquid:
Be first the pomegranate of 145:15:15:15:10:1000 by mass ratio: tomato: "Hami" melon: peach: grape: leaching filtrate was smashed in water mixing, culture medium is made with described filtrate, high-temperature sterilization 30 minutes at 115 DEG C, described culture medium is added in fermentation tank, then the saccharomycetic kind of liquid that the inoculum concentration inoculation step 1 being 5% of described filtrate by quality obtains, 48h is cultivated at the temperature of 28 DEG C, the volume of described fermentation tank is V cubic meter, throughput is 0.4V cubic meters per minute, speed of agitator 100rpm/min; Then the lactobacillus inoculation liquid that the inoculum concentration inoculation step 2 being 5% of described filtrate by quality obtains, continues to cultivate 5d (quiescent culture, without stirring, allows tank maintenance malleation in case microbiological contamination) and, to fermentation ends, obtains zymotic fluid at the temperature of 37 DEG C;
Step 4: zymotic fluid centrifugal treating is obtained ferment stoste:
Zymotic fluid step 3 obtained imports centrifuge, and through the centrifugal 10min of 6000rpm, gained supernatant is ferment stoste;
Step 5:
By mass ratio be: Cordyceps militaris extract: the ferment stoste mixing that the Cordyceps militaris extract of ferment stoste=250:1000 and step 4 obtain, maintains 30 minutes, carry out high-temperature sterilization, then carry out packing under 115 DEG C of environment.
Embodiment 2:
Step 1: prepare barms liquid:
Plant flow container and expand culture medium: YPD fluid nutrient medium, its mass percent is 1% yeast extract, 2% peptone, 2% glucose, 2% agar, and all the other are water, and YPD fluid nutrient medium is put into 0.5M
3in seeding tank, 50 minutes high-temperature sterilizations of 115 DEG C; Under aseptic technique, 1.5 kilograms of saccharomycete dry bacterium powders are dissolved in the sterilized water of 3 times, form bacterium liquid, described bacterium liquid is loaded serum bottle and is inoculated into 0.5M
3seeding tank, passes into aseptic compressed air at 30 DEG C, and throughput is 0.3 cubic meters per minute, cultivates 30h, until thalli growth to stopping during logarithmic phase latter stage cultivating, repeats the kind liquid that above-mentioned steps can obtain the yeast liquid of required quality;
Step 2: prepare lactobacillus inoculation liquid:
Plant flow container and expand culture medium: enrichment medium of wort, its mass percent is 10% brewer's wort, 0.5% soy peptone, 0.5% yeast extract, 1% beef extract, 0.2%K
2hPO
4,all the other are water, and enrichment medium of wort is put into 0.5M
3in seeding tank, 50 minutes high-temperature sterilizations of 115 DEG C; Be dissolved in by the dry bacterium powder of 1.5 kilograms of lactic acid bacterias in the sterilized water of 3 times under aseptic technique, bacterium liquid loads serum bottle and is inoculated into 0.5M
3seeding tank, 37 DEG C of constant temperature quiescent culture 36h, until thalli growth to stopping during logarithmic phase latter stage cultivating, can repeat the kind liquid that above-mentioned steps can obtain required lactic acid bacteria; Lactic acid bacteria in described step 2 is 1 kilogram of Bifidobacterium, 0.5 kilogram of streptococcus thermophilus.
Step 3: cultivation and fermentation liquid:
Be first the pomegranate of 145:15:15:15:10:1000 by mass ratio: tomato: "Hami" melon: peach: grape: leaching filtrate was smashed in water mixing, culture medium is made with described filtrate, high-temperature sterilization 30-50 minute at 115 DEG C, described culture medium is added in fermentation tank, then the saccharomycetic kind of liquid that the inoculum concentration inoculation step 1 being 10% of described filtrate by quality obtains, 48h is cultivated at the temperature of 28 DEG C, the volume of described fermentation tank is V cubic meter, throughput is 0.6V cubic meters per minute, speed of agitator 120rpm/min; Then be the lactobacillus inoculation liquid that the inoculum concentration inoculation step 2 of the 5%-10% of described filtrate obtains by quality, at the temperature of 37 DEG C, continue to cultivate 7d (quiescent culture, without stirring, allows tank maintenance malleation in case microbiological contamination), to fermentation ends, obtaining zymotic fluid;
Step 4: zymotic fluid centrifugal treating is obtained ferment stoste:
Zymotic fluid step 3 obtained imports centrifuge, and through the centrifugal 10min of 6000rpm, gained supernatant is ferment stoste;
Step 5:
By mass ratio be: Cordyceps militaris extract: the ferment stoste mixing that the Cordyceps militaris extract of ferment stoste=250:1000 and step 4 obtain, maintains 50 minutes, carry out high-temperature sterilization, then carry out packing under 115 DEG C of environment.
The evaluation and test volunteer that participation Cordyceps militaris ferment takes cosmetic result has 30 people, and sooner or later respectively taking 1 time, each 100-200ml for each person every day, 15 days is an evaluation and test cycle.Compare before and after taking, the average color cellulose content decline 15-30% of 30 people, skin elasticity increases 20-30%, and moisture content of skin increases 5-10%, and pore dia reduces 10-20%, and erythema reduces 10-20%
More than show and describe general principle of the present invention, principal character and advantage of the present invention.The technical staff of the industry should understand; the present invention is not restricted to the described embodiments; what describe in above-described embodiment and description just illustrates principle of the present invention; the present invention also has various changes and modifications without departing from the spirit and scope of the present invention, and these changes and improvements all fall in the claimed scope of the invention.Application claims protection domain is defined by appending claims and equivalent thereof.
Claims (2)
1. there is a preparation method for the Cordyceps militaris pomegranate ferment of cosmetic result, it is characterized in that: required step is as follows:
Step 1: prepare barms liquid:
Plant flow container and expand culture medium: YPD fluid nutrient medium, its mass percent is 1% yeast extract, 2% peptone, 2% glucose, 2% agar, and all the other are water, and YPD fluid nutrient medium is put into 0.5M
3in seeding tank, the 30-50 minute high-temperature sterilization of 115 DEG C; Under aseptic technique, 1.5 kilograms of saccharomycete dry bacterium powders are dissolved in the sterilized water of 3 times, form bacterium liquid, described bacterium liquid is loaded serum bottle and is inoculated into 0.5M
3seeding tank, passes into aseptic compressed air at 28-30 DEG C, and throughput is 0.2-0.3 cubic meters per minute, cultivates 20-30h, until thalli growth to stopping during logarithmic phase latter stage cultivating, repeats the kind liquid that above-mentioned steps can obtain the yeast liquid of required quality;
Step 2: prepare lactobacillus inoculation liquid:
Plant flow container and expand culture medium: enrichment medium of wort, its mass percent is 10% brewer's wort, 0.5% soy peptone, 0.5% yeast extract, 1% beef extract, 0.2%K
2hPO
4,all the other are water, and enrichment medium of wort is put into 0.5M
3in seeding tank, the 30-50 minute high-temperature sterilization of 115 DEG C; Be dissolved in by the dry bacterium powder of 1.5 kilograms of lactic acid bacterias in the sterilized water of 3 times under aseptic technique, bacterium liquid loads serum bottle and is inoculated into 0.5M
3seeding tank, 37 DEG C of constant temperature quiescent culture 24-36h, until thalli growth to stopping during logarithmic phase latter stage cultivating, can repeat the kind liquid that above-mentioned steps can obtain required lactic acid bacteria;
Step 3: cultivation and fermentation liquid:
Be first the pomegranate of 145:15:15:15:10:1000 by mass ratio: tomato: "Hami" melon: peach: grape: leaching filtrate was smashed in water mixing, culture medium is made with described filtrate, high-temperature sterilization 30-50 minute at 115 DEG C, described culture medium is added in fermentation tank, then be the saccharomycetic kind of liquid that the inoculum concentration inoculation step 1 of the 5%-10% of described filtrate obtains by quality, 48h is cultivated at the temperature of 28 DEG C, the volume of described fermentation tank is V cubic meter, throughput is (0.4-0.6) V cubic meters per minute, speed of agitator 100-120rpm/min; Then be the lactobacillus inoculation liquid that the inoculum concentration inoculation step 2 of the 5%-10% of described filtrate obtains by quality, continue to cultivate 5-7d to fermentation ends at the temperature of 37 DEG C, obtain zymotic fluid;
Step 4: zymotic fluid centrifugal treating is obtained ferment stoste:
Zymotic fluid step 3 obtained imports centrifuge, and through the centrifugal 10min of 6000rpm, gained supernatant is ferment stoste;
Step 5:
By mass ratio be: Cordyceps militaris extract: the ferment stoste mixing that the Cordyceps militaris extract of ferment stoste=250:1000 and step 4 obtain, maintains 30-50 minute, carry out high-temperature sterilization, then carry out packing under 115 DEG C of environment.
2. preparation method according to claim 1, is characterized in that: the lactic acid bacteria in described step 2 is 1 kilogram of Bifidobacterium, 0.5 kilogram of streptococcus thermophilus.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510865258.2A CN105361180A (en) | 2015-12-01 | 2015-12-01 | Preparation method of cordyceps militaris pomegranate enzyme with effect of beautifying features |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510865258.2A CN105361180A (en) | 2015-12-01 | 2015-12-01 | Preparation method of cordyceps militaris pomegranate enzyme with effect of beautifying features |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105361180A true CN105361180A (en) | 2016-03-02 |
Family
ID=55364266
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510865258.2A Pending CN105361180A (en) | 2015-12-01 | 2015-12-01 | Preparation method of cordyceps militaris pomegranate enzyme with effect of beautifying features |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105361180A (en) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20140057838A (en) * | 2012-11-05 | 2014-05-14 | 노길형 | Fermented foods process and production method |
| CN103960613A (en) * | 2014-05-09 | 2014-08-06 | 亳州千草药业有限公司 | Method for preparing fruit and vegetable enzymes |
| CN104738780A (en) * | 2015-04-21 | 2015-07-01 | 杭州曜拓贸易有限公司 | Integrated fruit enzyme and preparation method thereof |
| CN104856015A (en) * | 2015-04-24 | 2015-08-26 | 西藏月王生物技术有限公司 | A cordyceps sinensis enzyme and a preparation method thereof |
| CN104886572A (en) * | 2015-06-02 | 2015-09-09 | 广州加海生物科技有限公司 | A fruit and vegetable ferment beverage and a preparation method thereof |
-
2015
- 2015-12-01 CN CN201510865258.2A patent/CN105361180A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20140057838A (en) * | 2012-11-05 | 2014-05-14 | 노길형 | Fermented foods process and production method |
| CN103960613A (en) * | 2014-05-09 | 2014-08-06 | 亳州千草药业有限公司 | Method for preparing fruit and vegetable enzymes |
| CN104738780A (en) * | 2015-04-21 | 2015-07-01 | 杭州曜拓贸易有限公司 | Integrated fruit enzyme and preparation method thereof |
| CN104856015A (en) * | 2015-04-24 | 2015-08-26 | 西藏月王生物技术有限公司 | A cordyceps sinensis enzyme and a preparation method thereof |
| CN104886572A (en) * | 2015-06-02 | 2015-09-09 | 广州加海生物科技有限公司 | A fruit and vegetable ferment beverage and a preparation method thereof |
Non-Patent Citations (4)
| Title |
|---|
| 佟林峰等: "蛹虫草多糖配合果蔬酵素对抗氧化作用的研究", 《安徽农业科学》 * |
| 李艳: "《发酵工业概论》", 31 July 2011, 中国轻工业出版社 * |
| 沙莎等: "《动物微生物实验教程》", 31 August 2011, 西南师范大学出版社 * |
| 陈士怡等: "《酵母遗传学》", 31 October 1989, 科学出版社 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105361161A (en) | Making method of cordyceps militaris kiwi enzyme capable of enhancing immunity | |
| CN104719811A (en) | Method for producing pickle by shallow fermentation | |
| CN104152371A (en) | Preparation method of fermenting agent for direct vat set fermented milk | |
| CN106260152A (en) | A kind of Folium Camelliae sinensis ferment and preparation method thereof | |
| CN103431368A (en) | Preparation method and comprehensive utilization of submerged fermentation product of cordyceps sinensis liquid | |
| CN103243039A (en) | High density culturing method of lactobacillus paracasei | |
| CN114107403B (en) | Method for co-producing ellagic acid and biological feed by fermenting pericarpium Granati with microbial community | |
| CN104757114A (en) | Astaxanthin-containing health yoghourt and preparation method thereof | |
| CN105476021A (en) | Preparation method of cordyceps militaris cantaloupe enzyme with beauty treatment effect | |
| CN105476001A (en) | Manufacturing method of immunity-improving cordyceps militaris grape enzyme | |
| CN105861367A (en) | Pig manure methane fermenting agent formula and preparation method thereof | |
| CN108949629A (en) | A kind of composite ferment and the preparation method and application thereof | |
| CN108771115A (en) | A kind of GABA enrichments powder, preparation method thereof | |
| CN105533680A (en) | Preparation method of anti-fatigue cordyceps militaris Hami melon ferment | |
| CN105533679A (en) | Preparation method of anti-fatigue cordyceps militaris tomato ferment | |
| CN105361165A (en) | Preparation method of cordyceps militaris Hami melon enzyme with effect of reducing weight | |
| CN105361162A (en) | Making method of cordyceps militaris Hami melon enzyme capable of retarding senile dementia | |
| CN105495573A (en) | Preparation process of cordyceps militaris and bitter orange enzyme with weight loss effect | |
| CN106035985A (en) | Method for producing single cell proteins by using processed waste from mixed bacteria liquid fermentation of yellow wine | |
| CN105433385A (en) | Preparation method of immunity-improving cordyceps militaris and tomato enzyme | |
| CN105602821A (en) | Lentinus edodes mycelia-black garlic vinegar | |
| CN105361163A (en) | Preparation method of cordyceps militaris kiwi enzyme with effect of beautifying features | |
| CN105495574A (en) | Preparation method of cordyceps militaris and tomato enzyme with cosmetic effect | |
| CN105495610A (en) | Preparation method of cordyceps militaris and apple enzyme capable of improving immunity | |
| CN105361180A (en) | Preparation method of cordyceps militaris pomegranate enzyme with effect of beautifying features |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20160302 |
|
| WD01 | Invention patent application deemed withdrawn after publication |