CN105273430A - Trichosanthes kirilowii yellow pigment extraction technology - Google Patents
Trichosanthes kirilowii yellow pigment extraction technology Download PDFInfo
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- CN105273430A CN105273430A CN201410341181.4A CN201410341181A CN105273430A CN 105273430 A CN105273430 A CN 105273430A CN 201410341181 A CN201410341181 A CN 201410341181A CN 105273430 A CN105273430 A CN 105273430A
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- yellow pigment
- snakegourd
- trichosanthes kirilowii
- feed liquid
- ethanol
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Abstract
The present invention relates to a trichosanthes kirilowii yellow pigment extraction technology to solve the problems of being insoluble, different to extract and low in extraction efficiency of trichosanthes kirilowii yellow pigment, the trichosanthes kirilowii is taken, seeded and peeled, trichosanthes kirilowii pulp is crushed, added with deionized water for mixing, pH is adjusted with citric acid, pectinase and cellulase are added for enzymatic hydrolysis, filter cake is obtained by vacuum filtration, the filter cake is added with ethanol and dissolved, then put in 50 DEG C water bath for 90 minutes, and filtered to obtain filtrate, and the filtrate is concentrated under reduced pressure and is dried in vacuum to obtain the trichosanthes kirilowii yellow pigment. A dual enzymatic method is used for destruction of pectin and cellulose cell wall for release of intracellular yellow pigment, so that the extraction is easy, the yield is improved, the viscosity of the extract can be reduced, and the extract is easy to filter.
Description
Technical field
The invention belongs to natural plant pigment and extract preparing technical field, be specifically related to a kind of snakegourd yellow pigment extraction process.
Background technology
Snake gourd Curcurbitaceae herbaceous perennial vine plant, national most area all has distribution.Yellow pigment containing xenthophylls and carotene in mature fruit has very strong resistance of oxidation, also be the raw material being used for the treatment of ocular fundus pathology medicine simultaneously, research and develop the developing direction that natural, safe, multi-functional pigment is current food, medicine industry.
Snakegourd is from ancient times to the present extensively planted by China, and its fruit, seed, root all can enter separately medicinal.The skin of mature fruit, taste is sweet, cold in nature, has moistening the lung and resolving the phlegm, falls effect of fiery cough-relieving.Its flesh major ingredient is pectin and cellulose family composition, and with general solvent extraction, most snakegourd yellow pigment is difficult to stripping.
As can be seen here, prior art needs further improvement and develops.
Summary of the invention
Going out for solving snakegourd yellow pigment indissoluble, not easily extracting, the problem that extraction yield is low, the invention provides a kind of extraction process of snakegourd yellow pigment.
Technical scheme of the present invention is: get snakegourd, remove seed, peeling, by snakegourd flesh, pulverize, obtain feed liquid, add deionized water, the weightmeasurement ratio of feed liquid and deionized water is 1:18, after mixing, be citric acid adjust pH=4.0 ~ 6.0 of 10% again by mass concentration, obtain compound, add the polygalacturonase of feed liquid weight 0.1 ~ 0.5% and the cellulase of feed liquid weight 0.3-1.0%, then, enzymolysis 3 hours under the condition of 50 DEG C ~ 60 DEG C, vacuum filtration, obtain filter cake, namely filter cake is pigment part, add the dissolve with ethanol that mass concentration is 95%, the weightmeasurement ratio of feed liquid and 95% ethanol is 1:18, 60 DEG C of water-baths 90 minutes, filter, obtain filtrate, by filtrate reduced in volume, vacuum-drying, obtain.
The enzyme activity of described polygalacturonase is 50,000 u/g.
The enzyme activity of described polygalacturonase is 10,000 u/g.
Beneficial effect acquired by the present invention
The present invention makes 90% water-fast pigment in snakegourd flesh fully discharge by double-enzyme method, solves the defect that traditional extraction process extraction yield is very low.
Snakegourd flesh to remove after seed mainly colloid and cellulosic material, and pigment is wrapped wherein.The present invention adopts double-enzyme method to destroy colloid and cellulosic cell walls, and yellow pigment in cell is discharged, thus is easy to extract, and improves yield.And the viscosity of extracting solution can be reduced, easily filter.
The invention solves yellow pigment in snakegourd flesh and be difficult to the problem of stripping, pectin substance in flesh, fibrination, is wrapped in pigment, causes the technical barrier that the extraction yield of snakegourd yellow pigment is low.
Embodiment
Embodiment 1
Get snakegourd, remove seed, peeling, snakegourd flesh is put into food-liquid grinder pulverize, obtain feed liquid, take feed liquid 10g, add 150ml deionized water, after mixing, be that 10% citric acid is adjusted to pH value=4.0 by mass concentration again, obtain compound, then the cellulase of 0.05g polygalacturonase and 0.1g is added, enzymolysis 3 hours under the condition of 60 DEG C, vacuum filtration, namely filter cake is pigment part, add the dissolve with ethanol that 200ml mass concentration is 95%, put in water-bath, 60 DEG C are heated 90 minutes, cross and filter impurity, get filtrate 1ml, 25ml is settled to the ethanol that mass concentration is 95%, at 438nm place, survey its light absorption value A=0.72, after residual filtrate concentrating under reduced pressure reclaims ethanol, vacuum-drying obtains finished product 1.1g, one time extraction yield is 11%.
The enzyme activity of described polygalacturonase is 50,000 u/g.
The enzyme activity of described polygalacturonase is 10,000 u/g.
Embodiment 2
Get snakegourd, peeling, snakegourd flesh is put into food-liquid grinder pulverize, obtain feed liquid, take feed liquid 10g, add 150ml deionized water, after mixing, be that 10% citric acid is adjusted to pH value=5.3 by mass concentration again, obtain compound, then the cellulase of 0.03g polygalacturonase and 0.07g is added, enzymolysis 3 hours under the condition of 60 DEG C, vacuum filtration, namely filter cake is pigment part, add the dissolve with ethanol that 200ml mass concentration is 95%, put in water-bath, 50 DEG C are heated 90 minutes, cross and filter impurity, get filtrate 1ml, 25ml is settled to the ethanol that mass concentration is 95%, at 438nm place, survey its light absorption value A=0.65, after residual filtrate concentrating under reduced pressure reclaims ethanol, vacuum-drying, get product 0.89g, one time extraction yield is 8.9%.
The enzyme activity of described polygalacturonase is 50,000 u/g.
The enzyme activity of described polygalacturonase is 10,000 u/g.
Embodiment 3
Get snakegourd, remove seed, peeling, snakegourd flesh is put into food-liquid grinder pulverize, obtain feed liquid, take feed liquid 10g, add 150ml deionized water, after mixing, be that 10% citric acid is adjusted to pH value=6.0 by mass concentration again, then the cellulase of 0.01g polygalacturonase and 0.03g is added, enzymolysis 3 hours under the condition of 55 DEG C, vacuum filtration, namely filter cake is pigment part, add the dissolve with ethanol that 200ml mass concentration is 95%, put in water-bath, 60 DEG C are heated 90 minutes, cross and filter impurity, filter, get filtrate 1ml, 25ml is settled to the ethanol that mass concentration is 95%, at 438nm place, measure its light absorption value A=0.66, after residual filtrate concentrating under reduced pressure, vacuum-drying obtains finished product 0.92g, one time extraction yield is 9.2%.
The enzyme activity of described polygalacturonase is 50,000 u/g.
The enzyme activity of described polygalacturonase is 10,000 u/g.
Comparison example
Get snakegourd flesh slag to put into food-liquid grinder and pulverize, take snakegourd flesh slurry 10g, add deionized water by the solid-liquid ratio of 1g:15ml, PH=5.8 is adjusted to, 50 DEG C of-60 DEG C of heating in water bath 30 hours, cooling with 10% citric acid, lose pigment to separate out, vacuum filtration, sad filter.Filter cake takes out and puts into 500ml tool plug triangular flask, add ethanol 60 DEG C of water-baths 90 minutes of 200ml95%, filter, get filtrate 1ml, be settled to 25ml with the ethanol of 95%, at 438nm place, measure its light absorption value A=0.37, after residual filtrate concentrating under reduced pressure, vacuum-drying obtains finished product 0.56g, and one time extraction yield is 5.6%.
From above embodiment and comparative example, we can find out advantage of the present invention, and the present invention selects suitable enzyme dosage, PH, solid-liquid ratio, temperature and enzymolysis time, be easy to filter operation, and extraction yield is high, than without double-enzyme method, directly improve nearly one times of yield with 95% extraction using alcohol.
Claims (3)
1. the extracting method of a snakegourd yellow pigment, it is characterized in that: get snakegourd, remove seed, peeling, by snakegourd flesh, pulverize, obtain feed liquid, add deionized water, the weightmeasurement ratio of feed liquid and deionized water is 1:18, after mixing, be citric acid adjust pH=4.0 ~ 6.0 of 10% again by mass concentration, obtain compound, add the polygalacturonase of feed liquid weight 0.1 ~ 0.5% and the cellulase of feed liquid weight 0.3-1.0%, then, enzymolysis 3 hours under the condition of 50 DEG C ~ 60 DEG C, vacuum filtration, obtain filter cake, namely filter cake is pigment part, add the dissolve with ethanol that mass concentration is 95%, the weightmeasurement ratio of feed liquid and 95% ethanol is 1:18, 60 DEG C of water-baths 90 minutes, filter, obtain filtrate, by filtrate reduced in volume, vacuum-drying, obtain.
2. the extracting method of snakegourd yellow pigment according to claim 1, is characterized in that, the enzyme activity of described polygalacturonase is 50,000 u/g.
3. the extracting method of snakegourd yellow pigment according to claim 1, is characterized in that, the enzyme activity of described polygalacturonase is 10,000 u/g.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410341181.4A CN105273430A (en) | 2014-07-18 | 2014-07-18 | Trichosanthes kirilowii yellow pigment extraction technology |
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| CN201410341181.4A CN105273430A (en) | 2014-07-18 | 2014-07-18 | Trichosanthes kirilowii yellow pigment extraction technology |
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| CN105273430A true CN105273430A (en) | 2016-01-27 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111317691A (en) * | 2020-02-12 | 2020-06-23 | 南京中医药大学 | Trichosanthes kirilowii flesh total yellow pigment with whitening effect and preparation method and application thereof |
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2014
- 2014-07-18 CN CN201410341181.4A patent/CN105273430A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111317691A (en) * | 2020-02-12 | 2020-06-23 | 南京中医药大学 | Trichosanthes kirilowii flesh total yellow pigment with whitening effect and preparation method and application thereof |
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Application publication date: 20160127 |