CN105238097A - Method for supercritical carbon dioxide extraction of green pigment in snakegourd seeds and pulp - Google Patents
Method for supercritical carbon dioxide extraction of green pigment in snakegourd seeds and pulp Download PDFInfo
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- CN105238097A CN105238097A CN201510747793.8A CN201510747793A CN105238097A CN 105238097 A CN105238097 A CN 105238097A CN 201510747793 A CN201510747793 A CN 201510747793A CN 105238097 A CN105238097 A CN 105238097A
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- seeds
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/54—Improvements relating to the production of bulk chemicals using solvents, e.g. supercritical solvents or ionic liquids
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- Coloring Foods And Improving Nutritive Qualities (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a method for supercritical carbon dioxide extraction of a green pigment in snakegourd seeds and pulp. The method comprises the following steps: cleaning snakegourd seeds and green pulp to remove impurities, drying, grinding, extracting at 30-40DEG C under 20-35MPa under a flow velocity of 40-80L/h with supercritical fluid carbon dioxide as an extractant for 1-2h, adding 60-95% ethanol accounting for 30-50% of the mass of the snakegourd seeds and pulp as an entrainer through a pump in the extraction process, adjusting the pressure of a separator to 5-10MPa and the temperature to 20-25DEG C, and obtaining the snakegourd pulp green pigment from a separation kettle. The supercritical carbon dioxide fluid extraction method improves the quality and the bioactivity of the above obtained product, especially greatly improves the antibacterial property, has a strong inhibition effect on Escherichia coli, salmonella, Staphylococcus aureus and other pathogenic bacteria, is suitable for being used as a food additive, and has wide application prospect.
Description
Technical field
The present invention relates to a kind of method that supercritical co extracts marennin from discarded Seeds of Trichosanthes kirilowii flesh.
Background technology
Snake gourd Curcurbitaceae snake gourd per nnial herb is the traditional Chinese medicine material that China commonly uses.The fruit, skin, benevolence, root, stem, leaf etc. of snakegourd all can be used as medicine, and have coronary artery dilator, antitumor, antibacterial, anti-ageing, the effect such as anti-AIDS, suppression platelet aggregation, antiulcer agent.Snakegourd is mainly distributed in East Asia and Australia is northern, and plantation at home has expanded Hebei, Shanxi, Shaanxi to, the whole Yangtze valley, and the area such as south China, southwest.Because snakegourd has the advantage of pharmaceutical use and easy to raise, and along with continually developing in recent years to its product, the market conditions of snakegourd are become better and better, and also more and more receive the concern of investigator.
Seeds of Trichosanthes kirilowii is the position of snakegourd most worthy, is rich in the many kinds of substances such as grease, protein, triterpene glycoside, amino acid, sterol in Seeds of Trichosanthes kirilowii, and wherein protein, fat and the nutrient composition content such as total reducing sugar reach more than 80%, have higher nutritive value.
During existing Seeds of Trichosanthes kirilowii is produced, Seeds of Trichosanthes kirilowii flesh takes out from fruit together with Seeds of Trichosanthes kirilowii, in fermentation vat after fermentation, washing is removed snakegourd flesh and is obtained Seeds of Trichosanthes kirilowii, and snakegourd flesh is as waste disposal, therefore, a large amount of snakegourd fleshes (waste) can be produced in Seeds of Trichosanthes kirilowii production process, and containing a large amount of marennins in the green flesh of snakegourd, both water insoluble not perishable yet, cause environmental pollution and the wasting of resources.
The large quantity research of this seminar confirms: Seeds of Trichosanthes kirilowii flesh marennin is green powder product, have strong coloring force, lovely luster, tone nature, free from extraneous odour, heat-resisting, fast light, to foodstuff additive good stability, tone is not by the impact of pH value, to advantages such as human non-toxic's side effects, but it is low to there is extraction yield in the solvent-extraction process of routine, the defect of the Seeds of Trichosanthes kirilowii flesh marennin poor activity obtained, thus needs to seek new extracting method to improve Seeds of Trichosanthes kirilowii flesh marennin biological activity and yield.
Summary of the invention
Object of the present invention is exactly to overcome the deficiencies in the prior art, solve the snakegourd flesh produced when Seeds of Trichosanthes kirilowii is produced and treat as refuse process, cause the problem compared with large resource waste and environmental pollution, the method for snakegourd flesh marennin in the green flesh of a kind of supercritical carbon dioxide extraction snakegourd is provided.
For achieving the above object, the present invention is by the following technical solutions:
By green for Seeds of Trichosanthes kirilowii flesh cleaning impurity elimination, dry, after grinding, extraction agent is made with supercritical fluid carbon dioxide, temperature be 30-40 DEG C, pressure is 20-35MPa, flow velocity extracts 1-2h under being the condition of 40-80L/h, the ethanol adding the 60-95% of the green flesh quality 30-50% of Seeds of Trichosanthes kirilowii in extraction process with pump does entrainment agent, and the pressure regulating separator is 5-10MPa, temperature is 20-25 DEG C, obtains snakegourd flesh marennin from separating still.
The above-mentioned snakegourd flesh marennin directly obtained from separating still, through spectrophotometry, its marennin content reaches more than 60%, and product look valency is than high, and stability is also fine, and the solvent method raising 20%-30% that extraction yield is more conventional.
Compared with prior art, beneficial effect of the present invention is:
The green flesh of waste material snakegourd produced when producing with Seeds of Trichosanthes kirilowii is raw material, greatly reduces raw material production cost, has widened the bore of snakegourd secondary industry, for the mankind provide new marennin source.
Adopt the method for supercritical carbon dioxide extraction, improve quality and the biological activity of product, particularly anti-microbial property has the lifting of higher degree, to intestinal bacteria, Salmonellas and and the various pathogens such as staphylococcus glucose coccus have stronger restraining effect, be applicable to cooking foodstuff additive.
The present invention improves the economic benefit of Seeds of Trichosanthes kirilowii processing, and method is simple, and strong operability, has a extensive future.
Embodiment
embodiment 1:
By green for Seeds of Trichosanthes kirilowii flesh cleaning impurity elimination, dry, after grinding, extraction agent is made with supercritical fluid carbon dioxide, temperature be 38 DEG C, pressure is 30MPa, flow velocity extracts 1.2h under being the condition of 45L/h, do entrainment agent with the ethanol that pump adds 75% of the green flesh quality 39% of Seeds of Trichosanthes kirilowii in extraction process, the pressure regulating separator is 5.8MPa, temperature is 24 DEG C, obtains snakegourd flesh marennin from separating still.
The above-mentioned snakegourd flesh marennin directly obtained from separating still, through spectrophotometry, its marennin content reaches more than 60%, and product look valency is than high, and stability is also fine, and the solvent method raising 22% that extraction yield is more conventional.
embodiment 2:
By green for Seeds of Trichosanthes kirilowii flesh cleaning impurity elimination, dry, after grinding, extraction agent is made with supercritical fluid carbon dioxide, temperature be 32 DEG C, pressure is 23MPa, flow velocity extracts 1.9h under being the condition of 71L/h, do entrainment agent with the ethanol that pump adds 91% of the green flesh quality 48% of Seeds of Trichosanthes kirilowii in extraction process, the pressure regulating separator is 9MPa, temperature is 21 DEG C, obtains snakegourd flesh marennin from separating still.
The above-mentioned snakegourd flesh marennin directly obtained from separating still, through spectrophotometry, its marennin content reaches more than 60%, and product look valency is than high, and stability is also fine, and the solvent method raising 27% that extraction yield is more conventional.
embodiment 3:
By green for Seeds of Trichosanthes kirilowii flesh cleaning impurity elimination, dry, after grinding, extraction agent is made with supercritical fluid carbon dioxide, temperature be 35 DEG C, pressure is 34MPa, flow velocity extracts 1.2h under being the condition of 60L/h, do entrainment agent with the ethanol that pump adds 62% of the green flesh quality 31% of Seeds of Trichosanthes kirilowii in extraction process, the pressure regulating separator is 7MPa, temperature is 23 DEG C, obtains snakegourd flesh marennin from separating still.
The above-mentioned snakegourd flesh marennin directly obtained from separating still, through spectrophotometry, its marennin content reaches more than 60%, and product look valency is than high, and stability is also fine, and the solvent method raising 24% that extraction yield is more conventional.
Above-mentioned just embodiments of the invention, not do any pro forma restriction to the present invention.Any those of ordinary skill in the art, when not departing from technical solution of the present invention scope, can utilize the technology contents of above-mentioned announcement to make many possible variations and modification to technical solution of the present invention, or being revised as the Equivalent embodiments of equivalent variations.Therefore, every content not departing from technical solution of the present invention, according to the technology of the present invention essence to any simple modification made for any of the above embodiments, equivalent variations and modification, all should drop in the scope of technical solution of the present invention protection.
Claims (1)
1. the method for a supercritical carbon dioxide extraction Seeds of Trichosanthes kirilowii flesh medium green pigment, it is characterized in that: by green for Seeds of Trichosanthes kirilowii flesh cleaning impurity elimination, dry, after grinding, extraction agent is made with supercritical fluid carbon dioxide, temperature be 30-40 DEG C, pressure is 20-35MPa, flow velocity extracts 1-2h under being the condition of 40-80L/h, the ethanol adding the 60-95% of the green flesh quality 30-50% of Seeds of Trichosanthes kirilowii in extraction process with pump does entrainment agent, the pressure regulating separator is 5-10MPa, temperature is 20-25 DEG C, obtains snakegourd flesh marennin from separating still.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510747793.8A CN105238097A (en) | 2015-11-06 | 2015-11-06 | Method for supercritical carbon dioxide extraction of green pigment in snakegourd seeds and pulp |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510747793.8A CN105238097A (en) | 2015-11-06 | 2015-11-06 | Method for supercritical carbon dioxide extraction of green pigment in snakegourd seeds and pulp |
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| Publication Number | Publication Date |
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| CN105238097A true CN105238097A (en) | 2016-01-13 |
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| CN201510747793.8A Pending CN105238097A (en) | 2015-11-06 | 2015-11-06 | Method for supercritical carbon dioxide extraction of green pigment in snakegourd seeds and pulp |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103087548A (en) * | 2013-01-29 | 2013-05-08 | 安阳天尊生物工程有限公司 | Method for extracting trichosanthes kirilowii maxim uranidin |
| CN104419517A (en) * | 2013-09-11 | 2015-03-18 | 安阳天尊生物工程有限公司 | Method for extracting trichosanthes kirilowii seed oil and application of trichosanthes kirilowii seed oil in cosmetic filming |
-
2015
- 2015-11-06 CN CN201510747793.8A patent/CN105238097A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103087548A (en) * | 2013-01-29 | 2013-05-08 | 安阳天尊生物工程有限公司 | Method for extracting trichosanthes kirilowii maxim uranidin |
| CN104419517A (en) * | 2013-09-11 | 2015-03-18 | 安阳天尊生物工程有限公司 | Method for extracting trichosanthes kirilowii seed oil and application of trichosanthes kirilowii seed oil in cosmetic filming |
Non-Patent Citations (3)
| Title |
|---|
| "栝楼皮叶黄素的超临界CO2萃取及其稳定性研究";马志虎 等;《精细化工》;20091231;第26卷(第12期);1215-1220 * |
| 余丽芳 等: ""栝蒌瓤液成分分析与主要活性物质的制备"", 《安徽农业科学》 * |
| 马志虎 等: ""栝楼皮叶黄素的超临界CO2萃取及其稳定性研究"", 《精细化工》 * |
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Application publication date: 20160113 |