CN105231166A - Active ferment with high SOD content and preparation method thereof - Google Patents
Active ferment with high SOD content and preparation method thereof Download PDFInfo
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- CN105231166A CN105231166A CN201510632423.XA CN201510632423A CN105231166A CN 105231166 A CN105231166 A CN 105231166A CN 201510632423 A CN201510632423 A CN 201510632423A CN 105231166 A CN105231166 A CN 105231166A
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- ferment
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- inonotus obliquus
- stoste
- high sod
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses an active ferment with a high SOD content and a preparation method thereof. The preparation method comprises the following steps: (a) cutting different fruits and vegetables into slices, grinding the slices, and adding white sugar; (b) pulping edible fungus, extracting cordyceps militaris sporocarp and hericium erinaceus sporocarp to obtain extracts, and mixing extracts and edible fungus pulp to obtain edible/pharmaceutical fungus mixed liquid; (c) culturing inonotus obliquus to obtain inonotus obliquus fermentation broth; (d) individually using saccharomyces, bacillus aceticus , and lactobacillus to ferment fruits and vegetables and the edible/pharmaceutical fungus mixed liquid to obtain fruit and vegetable ferment original liquor and edible/pharmaceutical fungus ferment original liquor; (e) merging the inonotus obliquus fermentation broth, fruit and vegetable ferment original liquor, and edible/pharmaceutical fungus ferment original liquor to obtain the active ferment with a high SOD content. The reutilization of inonotus obliquus fermentation waste liquid is achieved, and the waste liquid can be effectively chelated with ferment to obtain the active ferment with a high SOD content. A long time fermentation, which lasts for about 30 days, is adopted and the active ferment can prominently improve the bioavailability of human body.
Description
Technical field
The invention belongs to field of fermentation engineering, particularly relate to a kind of high SOD active ferment and preparation method thereof.
Background technology
Inonotus obliquus (Inonotusobliquus), it is rare large-scale medicinal fungi, have another name called: CHAGA, English by name: Chaga etc., this Pseudomonas Basidiomycotina, Hymenomycetes, non-brown Zoopagales (aptychus Zoopagales or Aphyllophorales), Polyporaceae, brown transverse hole fungus belong to (fine pore fungi belongs to).Main parasitic in white birch, silvery birch, elm, under the bark of alder etc. or under the bark of vigor wood or after felling trees dried-up on.This bacterium seldom forms fructification, and its sterility fructification presents warty, and appearance is hard, and yellowish-brown, to black, has the fritter of obvious uneven cracking.Under being just born in bark, expose after decortication, tiling expansion, not of uniform size, be just white, the look that turns yellow gradually, filbert to brown, basidiospore is colourless extremely filbert, and meat bacteria organization is made up of brown setiform mycelia and faint yellow generative hyphae, bristle body crineous.Biological study shows, Inonotus obliquus grows very slow under wild state, wild varieties only grows and just has very high medical value in 10 ~ 15 years on the birch lived, and, every 20,000 birches approximately only have one raw Inonotus obliquus, Inonotus obliquus not yet realizes because of artificial domesticating cultivation, the U.S., Japan, Korea S has increased the research dynamics to Inonotus obliquus, Japan has proposed patent application protection in countries in the world, the U.S. is also classified as it as " special natural goods " following drink as extraterrestrial, Liquid Culture and Mycelium culture research are being entered by Korea S.Inonotus obliquus is the supplement of beverage, flavouring, biscuit and the processing such as Bread and Pastries, sausage class meat, Chinese medicine, is saturate food coloring raw material.Because Inonotus obliquus has effect to prevention SARS virus, the exploitation of research of Inonotus obliquus and products thereof has very wide application prospect.
Ferment is also called enzyme, is the catalyst of various biological respinse, and be a kind of protein with sp act, it is present in each physiological response system and organ-tissue, to use in catalysis and regulation and control body all biochemical reactions and metabolicly to carry out.In human body, the metabolism, new life, decomposition, digestion, synthesis etc. of every cell, all rely on ferment to complete the health that ferment determines us, more can determine the length in our life-span and short simultaneously.Therefore ferment is referred to as again " Source of life ".Ferment also has " selectivity ", and each ferment can only carry out by a kind of biochemical reaction of catalysis, and the ferment kind therefore in human body is up to thousands of kinds.Ferment be probiotic action in various food materials, the easy absorbing material of various Small molecular that food materials are resolved into, such as various monose, amino acid, vitamin, the small-molecule substance that mineral matter etc. easily absorb.Ferment usually with fruits and vegetables and edible mushroom for raw material, by adding different probios, some months at least, at most several years, process is complicated, and the time is long, easy living contaminants, and product quality is difficult to control.
Summary of the invention
The object of the invention is to overcome above-mentioned weak point and a kind of high SOD active ferment is provided.
Another object of the present invention is to provide the preparation method of this high SOD active ferment.
The object of the invention is to realize in the following manner:
A kind of high SOD active ferment, this high SOD active ferment prepares by the following method:
A) cut into slices by fruits and vegetables, smash to pieces, admix white sugar, sterilization, adds saccharomycete, acetobacter and lactic acid bacteria and ferments, obtain pectase stoste;
B) pulled an oar by edible mushroom, add white sugar, sterilization, obtains edible mushroom slurries; Fruiting bodies of cordyceps militaris and hericium erinaceus fruiting body are pulverized respectively, obtain leaching liquor respectively with hot water extraction, and fruiting bodies of cordyceps militaris leaching liquor, hericium erinaceus fruiting body leaching liquor and edible mushroom slurries are mixed to get edible and medical fungi mixed liquor; Add saccharomycete, acetobacter, lactobacillus-fermented obtains edible and medical fungi ferment stoste;
C) Inonotus obliquus is carried out cultivation and obtain Inonotus obliquus zymotic fluid;
D) Inonotus obliquus zymotic fluid, pectase stoste and edible and medical fungi ferment stoste are mixed, chelating more than 30 days, obtain high SOD active ferment.
Above-mentioned steps a) described in fruits and vegetables be selected from following at least 5 kinds: apple, dragon fruit, Kiwi berry, banana, pawpaw, tomato, radish, spinach, pumpkin, bottle gourd, the part by weight of often kind of fruit or vegetables and white sugar is 1:2-2:1, and preferred weight ratio is 1:1.
Above-mentioned steps b) described edible mushroom be selected from asparagus, mushroom, coprinus comatus, auricularia auriculajudae, mushroom, agrocybe, pleurotus eryngii any three or more, the part by weight of often kind of edible mushroom and white sugar is 1:2-2:1, and preferred weight ratio is 1:1.
Above-mentioned steps b) described leaching liquor specifically prepares in accordance with the following methods: fruiting bodies of cordyceps militaris powder and Hydnum erinaceus sporophore powder are added purified water respectively, lixiviate 3-4h under 80-95 DEG C of condition; The part by weight of often kind of fungus sporophore powder and purified water is 1:3-1:5, and preferred weight ratio is 1:4.
Above-mentioned steps c) Inonotus obliquus seed culture based formulas is: 2%-5% glucose, 1%-4% soybean cake powder, 0.1%-0.3% beef extract, 0.01%-0.1% peptone, 0.01-0.1%KH
2pO
4, 0.01-0.05%MgSO
4, pH6.0.
Above-mentioned steps c) in Inonotus obliquus condition of culture be: temperature 25-30 DEG C, rotating speed 100-200rpm, cultivate 5-8 days.
Step a) and step b) described in saccharomycete, acetobacter and lactic acid bacteria inoculum concentration be respectively according to volume ratio: 1%-1.5%, 1%-2%, 2%-3%, the inoculum concentration of preferred yeast bacterium, acetobacter and lactic acid bacteria is respectively according to volume ratio: 1.5%, 2%, 3%.
Step b) in fruiting bodies of cordyceps militaris leaching liquor, hericium erinaceus fruiting body leaching liquor and edible mushroom slurries three weight ratio be 1:1:1.
Step a) in the fermentation condition of pectase stoste be at 30 DEG C of bottom fermentation 15d, temperature is adjusted to 37 DEG C of 15d that ferment; Step b) in the fermentation condition of edible and medical fungi ferment stoste be after 30 DEG C of bottom fermentation 10-15d, temperature is adjusted to 37 DEG C of 15d-20d that ferment.
The preparation method of above-mentioned high SOD active ferment comprises the following steps:
A) fruits and vegetables are cut into slices, smash to pieces, admix white sugar, ultraviolet disinfection process; Add saccharomycete, acetobacter and lactic acid bacteria to ferment, obtain pectase stoste;
B) edible mushroom is pulled an oar, add white sugar, ultraviolet disinfection process; Fruiting bodies of cordyceps militaris and hericium erinaceus fruiting body are pulverized respectively, obtain leaching liquor respectively with hot water extraction, and fruiting bodies of cordyceps militaris leaching liquor, hericium erinaceus fruiting body leaching liquor and edible mushroom slurries are mixed to get edible and medical fungi mixed liquor; Add saccharomycete, acetobacter, lactobacillus-fermented obtains edible and medical fungi ferment stoste;
C) Inonotus obliquus is carried out cultivation and obtain Inonotus obliquus zymotic fluid;
D) Inonotus obliquus zymotic fluid, pectase stoste and edible and medical fungi ferment stoste are mixed, chelating more than 30 days, control temperature, at 10-20 DEG C, obtains high SOD active ferment.
The preparation method of above-mentioned high SOD active ferment, specifically comprises the following steps:
A) different fruits and vegetables are cut into slices, smash to pieces with high-speed homogenization machine, admix white sugar, ultraviolet disinfection process; Fruits and vegetables be selected from
At least 5 kinds in lower: apple, dragon fruit, Kiwi berry, banana, pawpaw, tomato, carrot, spinach, pumpkin, each 1 part of bottle gourd, the part by weight of often kind of fruit or vegetables and white sugar is 1:1; Add saccharomycete, acetobacter and lactic acid bacteria in fruits and vegetables mixed liquor to ferment, obtain pectase stoste; The inoculum concentration of saccharomycete, acetobacter and lactic acid bacteria is respectively: 1%-1.5%, 1%-2%, 2%-3%.
B) smash edible mushroom high-speed homogenization machine to pieces making beating, add 1 part of white sugar, ultraviolet disinfection process obtains edible mushroom slurries; Fruiting bodies of cordyceps militaris and hericium erinaceus fruiting body are pulverized respectively, use 80-95 DEG C of hot water extraction 3-4h respectively, obtain leaching liquor, the weight ratio of fruiting bodies of cordyceps militaris powder or Hydnum erinaceus sporophore powder and purified water is 1:4, and fruiting bodies of cordyceps militaris leaching liquor, hericium erinaceus fruiting body leaching liquor and edible mushroom slurries are mixed to get edible and medical fungi mixed liquor.Described edible mushroom be selected from asparagus, mushroom, coprinus comatus, auricularia auriculajudae, mushroom, agrocybe, pleurotus eryngii any three or more, add saccharomycete in edible and medical fungi mixed liquor, acetobacter, lactobacillus-fermented obtains edible and medical fungi ferment stoste; The inoculum concentration of described saccharomycete, acetobacter and lactic acid bacteria is respectively: 1%-1.5%, 1%-2%, 2%-3%.
That c) is carried out by Inonotus obliquus cultivating obtains Inonotus obliquus zymotic fluid; Condition of culture is: with 2%-5% glucose, 1%-4% soybean cake powder, 0.1%-0.3% beef extract, 0.01%-0.1% peptone, 0.01-0.1%KH
2pO
4, 0.01-0.05%MgSO
4, pH6.0 is formula, preparation culture medium, culture medium 121 DEG C of moist heat sterilization 15-30min, and inoculation Inonotus obliquus inclined-plane seed 1-2 ring, 25-30 DEG C, rotating speed 100-200rp, cultivate 5-8 days, obtain Inonotus obliquus zymotic fluid.
E) merge Inonotus obliquus zymotic fluid, pectase stoste and edible and medical fungi ferment stoste, mix rear chelating and within more than 30 days, obtain high SOD active ferment.
The mixed proportion of Inonotus obliquus zymotic fluid, pectase stoste and edible and medical fungi ferment stoste three is 1:1:1.
High SOD active ferment of the present invention can be applied preparing in food, health food.
SOD Sudismase is that human life removes interior free yl, ensures the First Line defence pioneer of quality of life.Inonotus obliquus is rich in polysaccharide, inonotus obliquus, Inonotus obliquus alcohol, multiple triterpene substance etc. 215 kinds more than.Wherein SOD Sudismase content is first of nature all things on earth, every gram of Inonotus obliquus SOD active ferment content up to 35000 units, be 23 times of Agricus blazei, 25 times of Hericium erinaceus, glossy ganoderma 55 times, 318 times of Phellinus, 250 times of spinach.
The present invention's saccharomycete used can glucose fermentation, fructose, by saccharide converted one-tenth alcohol, meanwhile, is also rich in multivitamin, mineral matter and multiple enzyme in yeast cells, can promotes its digested absorption.In addition it is also containing multiple active material rarely known by the people, as ergosterol, glutathione, superoxide dismutase, coacetylase etc.
Alcohol oxidation is mainly the organic acids such as acetic acid, malic acid, butanedioic acid, citric acid by the present invention's acetic acid bacteria used, the amino acid also containing multiple needed by human body, vitamin and bioactivator.Abundant organic acid, effectively can maintain the acid-base balance in body.Produce alkaline matter after the mineral metabolisms such as the potassium in fruit vinegar, zinc, maintain blood alkalescence.
The present invention lactic acid bacteria used can produce lactic acid from the sweat of glucose or lactose, promotes the absorption of protein, monose and the nutriment such as calcium, magnesium, produces a large amount of benefit materials such as vitamin B complex.Make the formation of gut flora that useful change occur, improve human gastrointestinal tract function, recover colony balance in human body intestinal canal, suppress the breeding of spoilage organisms, clear up the toxin that spoilage organisms produces, remove enteron aisle rubbish, strengthen body immunity and resistance.Improve SOD enzyme activity, eliminate human free radical, tool is anti-ageing, effect of promoting longevity.
The present invention pass through the combined ferment of useful bacterial strain, obtain the beneficiating ingredients such as a large amount of amino acid, Small molecular functional sugar, vitamin, add Inonotus obliquus zymotic fluid again, it contains the materials such as a large amount of triterpenes, polysaccharide and pigment, there is the multiple health cares such as nourishing the stomach, beauty treatment, weight reducing, develop immunitypty, and achieve the recycling of Inonotus obliquus fermented waste fluid.
Beneficial effect of the present invention compared with the prior art: Inonotus obliquus zymotic fluid Chang Zuowei discarded object abandons, it but effectively utilizes by the present invention, not only utilize its beneficial agents, utilize again the high SOD of Inonotus obliquus zymotic fluid active, obtain high SOD active ferment with the effective chelating of ferment.In addition, fermentation when the present invention adopts about 30 days long, significantly improves the bioavilability of human body.
Detailed description of the invention
The present invention is further illustrated below by way of specific embodiment.But the detail of embodiment only for explaining the present invention, should not be construed as limited overall technical solution.
Embodiment 1
1, the seed culture of Inonotus obliquus
Inonotus obliquus seed culture based formulas is: glucose 2%, soybean cake powder 1%, beef extract 0.3%, peptone 0.05%, KH
2pO
40.05%, MgSO
40.02%, pH6.0; Culture medium 121 DEG C of moist heat sterilization 20min, inoculate Inonotus obliquus inclined-plane seed 1-2 ring, 28 DEG C, rotating speed 160rpm after being cooled to normal temperature, cultivate 7 days, obtain shake-flask seed.
2, the seed culture of yeast
Saccharomycete seed culture medium prescription is: glucose 2%, yeast extract 0.5%, peptone 0.5%, NaCl0.05%, pH5-7, culture medium 121 DEG C of moist heat sterilization 20min, inoculation yeast test tube strains 1-2 ring after cooling, 28-30 DEG C, upper shaking table 150rpm, cultivates 3 days, obtains shake-flask seed.
3, the seed culture of acetobacter
Acetobacter seed culture based formulas is: glucose 3%, dusty yeast 3%, PH5.5, adds alcohol (food grade) 4% after sterilizing; Culture medium 121 DEG C of moist heat sterilization 20min, inoculate acetobacter inclined-plane seed 1-2 ring, upper shaking table, 28-33 DEG C, rotating speed 170rpm after being cooled to normal temperature, cultivate 5 days, obtain shake-flask seed.
4, lactic acid bacteria seed culture
Lactic acid bacteria seed culture medium prescription is: glucose 3%, peptone 2%, dusty yeast 1%, Tween-80 0.05%, diammonium hydrogen citrate 0.2%, MnSO
4h
2o0.002%, MgSO
47H
2o0.025%, K
2hPO
40.2%, pH value 5.5-6.2; Culture medium 121 DEG C of moist heat sterilization 20min, inoculating lactic acid bacterium after cooling, 37 DEG C, micro-oxygen quiescent culture 5 days, obtains lactic acid bacteria seed.5, the preparation of fruit and vegetable ferment
Fruits and vegetables be selected from following at least 5 kinds: apple, dragon fruit, Kiwi berry, banana, pawpaw, tomato, carrot, spinach, pumpkin, each 2 kilograms of bottle gourd, smash to pieces with high-speed homogenization machine, add 2 kilograms of white sugar, ultraviolet disinfection process obtains fruits and vegetables mixed liquor.Add bacterial classification respectively by volume, i.e. (in fruits and vegetables mixed liquor for 100%): 1.5% saccharomycete, 2% acetobacter, 3% lactic acid bacteria, at 30 DEG C of bottom fermentation 15d, temperature is adjusted to 37 DEG C of fermentation 15d, obtains pectase stoste.
6, the preparation of edible mushroom ferment
Be selected from asparagus, mushroom, coprinus comatus, auricularia auriculajudae, mushroom, agrocybe, each 2 kilograms of pleurotus eryngii, smash to pieces with high-speed homogenization machine, add 2 kilograms of white sugar, ultraviolet disinfection process, obtain edible mushroom slurries.Choose 2 kilograms of fruiting bodies of cordyceps militaris powder and Hydnum erinaceus sporophore powder respectively, add the purified water of its 4 times of weight respectively, under 80-95 DEG C of condition, lixiviate 3-4h.By fruiting bodies of cordyceps militaris leaching liquor, hericium erinaceus fruiting body leaching liquor and edible mushroom slurries three mixing, bacterial classification is added respectively by (in mixed liquor for 100%) volume ratio, that is: 1.5% saccharomycete, 2% acetobacter, 3% lactic acid bacteria, at 30 DEG C of bottom fermentation 15d, temperature is adjusted to 37 DEG C of fermentation 15d, obtains edible and medical fungi ferment stoste.
7, the preparation of high SOD active ferment
By Inonotus obliquus zymotic fluid, pectase stoste and the edible and medical fungi ferment stoste part by weight according to 1:1:1, after mixing, chelating obtains high SOD active ferment for more than 30 days.According to " mensuration (GB/T5009.171-2003) that in health food, superoxide dismutase (SOD) is active ", this ferment SOD enzyme activity is 18000U/mL, wherein the SOD enzyme activity of Inonotus obliquus zymotic fluid, pectase stoste and edible and medical fungi ferment stoste is respectively 14500U/mL, 13000U/mL and 12500U/mL, and comparatively three kinds of ferment SOD enzyme activity improve 24%, 38% and 44% respectively.
Embodiment 2
1, the seed culture of Inonotus obliquus
Inonotus obliquus seed culture based formulas is: glucose 5%, soybean cake powder 2%, beef extract 0.1%, peptone 0.01%, KH
2pO
40.01%, MgSO
40.01%, pH6.0; Culture medium 121 DEG C of moist heat sterilization 20min, inoculate Inonotus obliquus inclined-plane seed 1-2 ring, 30 DEG C, rotating speed 160rpm after being cooled to normal temperature, cultivate 6 days, obtain shake-flask seed.
2, the seed culture of yeast
Saccharomycete seed culture medium prescription is: glucose 2%, yeast extract 0.5%, peptone 0.5%, NaCl0.05%, pH5-7, culture medium 121 DEG C of moist heat sterilization 20min, inoculation yeast test tube strains 1-2 ring after cooling, 28-30 DEG C, upper shaking table 150rpm, cultivates 3 days, obtains shake-flask seed.
3, the seed culture of acetobacter
Acetobacter seed culture based formulas is: glucose 2%, dusty yeast 5%, PH5.5, adds alcohol (food grade) 4% after sterilizing; Culture medium 121 DEG C of moist heat sterilization 20min, inoculate acetobacter inclined-plane seed 1-2 ring, upper shaking table, 28-33 DEG C, rotating speed 170rpm after being cooled to normal temperature, cultivate 5 days, obtain shake-flask seed.
4, lactic acid bacteria seed culture
Lactic acid bacteria seed culture medium prescription is: glucose 3%, peptone 2%, dusty yeast 1%, Tween-80 0.05%, diammonium hydrogen citrate 0.2%, MnSO
4h
2o0.002%, MgSO
47H
2o0.025%, K
2hPO
40.2%, pH value 5.5-6.2; Culture medium 121 DEG C of moist heat sterilization 20min, inoculating lactic acid bacterium after cooling, 37 DEG C, micro-oxygen quiescent culture 5 days, obtains lactic acid bacteria seed.7, the preparation of fruit and vegetable ferment
Fruits and vegetables be selected from following at least 5 kinds: apple, dragon fruit, Kiwi berry, banana, pawpaw, tomato, carrot, spinach, pumpkin, each 2 kilograms of bottle gourd, smash to pieces with high-speed homogenization machine, add 2 kilograms of white sugar, ultraviolet disinfection process obtains fruits and vegetables mixed liquor.Add composite bacteria respectively by volume, i.e. (in fruits and vegetables mixed liquor for 100%): 1% saccharomycete, 1% acetobacter, 2% lactic acid bacteria, at 30 DEG C of bottom fermentation 10d, temperature is adjusted to 37 DEG C of fermentation 20d, obtains pectase stoste.8, the preparation of edible mushroom ferment
Be selected from asparagus, mushroom, coprinus comatus, auricularia auriculajudae, mushroom, agrocybe, each 2 kilograms of pleurotus eryngii, smash to pieces with high-speed homogenization machine, add 2 kilograms of white sugar, ultraviolet disinfection process, obtain edible mushroom slurries.Choose 2 kilograms of fruiting bodies of cordyceps militaris powder and Hydnum erinaceus sporophore powder respectively, add the purified water of its 4 times of weight respectively, under 80-95 DEG C of condition, lixiviate 3-4h.By fruiting bodies of cordyceps militaris leaching liquor, hericium erinaceus fruiting body leaching liquor and edible mushroom slurries three mixing, composite bacteria is added respectively by (in mixed liquor for 100%) volume ratio, that is: 1% saccharomycete, 1% acetobacter, 2% lactic acid bacteria, at 30 DEG C of bottom fermentation 15d, temperature is adjusted to 37 DEG C of fermentation 15d, obtains edible and medical fungi ferment stoste.
7, the preparation of high SOD active ferment
By Inonotus obliquus zymotic fluid, pectase stoste and the edible and medical fungi ferment stoste part by weight according to 1:1:1, after mixing, chelating obtains high SOD active ferment for more than 30 days.According to " mensuration (GB/T5009.171-2003) that in health food, superoxide dismutase (SOD) is active ", this ferment SOD enzyme activity is 20000U/mL, wherein the SOD enzyme activity of Inonotus obliquus zymotic fluid, pectase stoste and edible and medical fungi ferment stoste is respectively 15000U/mL, 12000U/mL and 13000U/mL, and comparatively three kinds of ferment SOD enzyme activity improve 33%, 66% and 54% respectively.
Claims (10)
1. a high SOD active ferment, is characterized in that, this high SOD active ferment prepares by the following method:
A) cut into slices by fruits and vegetables, smash to pieces, admix white sugar, sterilization, adds saccharomycete, acetobacter and lactic acid bacteria and ferments, obtain pectase stoste;
B) pulled an oar by edible mushroom, add white sugar, sterilization, obtains edible mushroom slurries; Fruiting bodies of cordyceps militaris and hericium erinaceus fruiting body are pulverized respectively, obtain leaching liquor respectively with hot water extraction, and fruiting bodies of cordyceps militaris leaching liquor, hericium erinaceus fruiting body leaching liquor and edible mushroom slurries are mixed to get edible and medical fungi mixed liquor; Add saccharomycete, acetobacter, lactobacillus-fermented obtains edible and medical fungi ferment stoste;
C) Inonotus obliquus is carried out cultivation and obtain Inonotus obliquus zymotic fluid;
D) Inonotus obliquus zymotic fluid, pectase stoste and edible and medical fungi ferment stoste are mixed in proportion, chelating more than 30 days, obtain high SOD active ferment, the mixed proportion of Inonotus obliquus zymotic fluid, pectase stoste and edible and medical fungi ferment stoste three is 1:1:1.
2. high SOD active ferment according to claim 1, it is characterized in that, described step a) described in fruits and vegetables be selected from following at least 5 kinds: apple, dragon fruit, Kiwi berry, banana, pawpaw, tomato, radish, spinach, pumpkin, bottle gourd, the part by weight of often kind of fruit or vegetables and white sugar is 1:2-2:1, and preferred weight ratio is 1:1.
3. high SOD active ferment according to claim 1, it is characterized in that, described step b) described edible mushroom be selected from asparagus, mushroom, coprinus comatus, auricularia auriculajudae, mushroom, agrocybe, pleurotus eryngii any three or more, the part by weight of often kind of edible mushroom and white sugar is 1:2-2:1, and preferred weight ratio is 1:1.
4. high SOD active ferment according to claim 1, it is characterized in that, described step b) described leaching liquor specifically prepares in accordance with the following methods: fruiting bodies of cordyceps militaris powder and Hydnum erinaceus sporophore powder are added purified water respectively, lixiviate 3-4h under 80-95 DEG C of condition; The part by weight of often kind of fungi entity powder and purified water is 1:3-1:5, and preferred weight ratio is 1:4.
5. high SOD active ferment according to claim 1, is characterized in that, described step c) Inonotus obliquus seed culture based formulas is: 2%-5% glucose, 1%-4% soybean cake powder, 0.1%-0.3% beef extract, 0.01%-0.1% peptone, 0.01-0.1%KH
2pO
4, 0.01-0.05%MgSO
4, pH6.0.
6. high SOD active ferment according to claim 1, is characterized in that, described step c) in Inonotus obliquus condition of culture be: temperature 25-30 DEG C, rotating speed 100-200rpm, cultivate 5-8 days.
7. high SOD active ferment according to claim 1, it is characterized in that, step a) and step b) described in saccharomycete, acetobacter and lactic acid bacteria inoculum concentration be respectively according to volume mass ratio: 1%-1.5%, 1%-2%, 2%-3%, the inoculum concentration of preferred yeast bacterium, acetobacter and lactic acid bacteria is respectively according to volume mass ratio: 1.5%, 2%, 3%.
8. high SOD active ferment according to claim 1, is characterized in that, step b) in fruiting bodies of cordyceps militaris leaching liquor, hericium erinaceus fruiting body leaching liquor and edible mushroom slurries three weight ratio be 1:1:1.
9. high SOD active ferment according to claim 1, is characterized in that, step a) in the fermentation condition of pectase stoste be at 30 DEG C of bottom fermentation 10-15d, temperature is adjusted to 37 DEG C of 15-20d that ferment; Step b) in the fermentation condition of edible and medical fungi ferment stoste be after 30 DEG C of bottom fermentation 10-15d, temperature is adjusted to 37 DEG C of 15-20d that ferment.
10. the preparation method of high SOD active ferment according to claim 1, is characterized in that the method comprises the following steps:
A) fruits and vegetables are cut into slices, smash to pieces, admix white sugar, ultraviolet disinfection process; Add saccharomycete, acetobacter and lactic acid bacteria to ferment, obtain pectase stoste;
B) edible mushroom is pulled an oar, add white sugar, ultraviolet disinfection process; Fruiting bodies of cordyceps militaris and hericium erinaceus fruiting body are pulverized respectively, obtain leaching liquor respectively with hot water extraction, and fruiting bodies of cordyceps militaris leaching liquor, hericium erinaceus fruiting body leaching liquor and edible mushroom slurries are mixed to get edible and medical fungi mixed liquor; Add saccharomycete, acetobacter, lactobacillus-fermented obtains edible and medical fungi ferment stoste;
C) Inonotus obliquus is carried out cultivation and obtain Inonotus obliquus zymotic fluid;
D) Inonotus obliquus zymotic fluid, pectase stoste and edible and medical fungi ferment stoste are mixed, chelating more than 30 days, control temperature, at 10-20 DEG C, obtains high SOD active ferment.
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