CN105211565A - Feed of a kind of pre-insect protected line river blackfish body surface ulcer and preparation method thereof - Google Patents
Feed of a kind of pre-insect protected line river blackfish body surface ulcer and preparation method thereof Download PDFInfo
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Abstract
The present invention relates to a kind of fish meal, particularly a kind of feed of pre-insect protected line river blackfish body surface ulcer; The invention still further relates to the preparation method of the feed of pre-insect protected line river blackfish body surface ulcer.The feed of pre-insect protected line river blackfish body surface ulcer of the present invention, comprise each component of following masses number: fish meal 20-30 part, earthworm powder 8-15 part, squid powder 4-10 part, rice bran 6-12 part, saururus chinensis 3-7 part, water melon frost 3-8 part, snake slough 2-6 part, gold boils careless 3-7 part, cuttle bone 2-5 part, pollen pini 2-6 part, concha meretricis seu cylinae 1-5 part, calamine 1-4 part, granatum 2-6 part, sealwort 1-4 part, olive oil 1-4 part, compound vitamin 1-4 part, after feeding worm line river blackfish, feed coefficient is low, specific growth rate is high, and resistance to surface ulcer ability is strong.
Description
Technical field
The present invention relates to a kind of fish meal, particularly a kind of feed of pre-insect protected line river blackfish body surface ulcer; The invention still further relates to a kind of above-mentioned pre-insect protected line river blackfish body surface ulcer feed producing method.
Background technology
Worm line river blackfish fish body is spindle, and back of head slightly swells, and long is 1/3 of total length, and mouth end position, schistostoma is large.Naked eyes see body surface like without squama, and with tactile smooth of hand, actual body is by carefully small and dense ctenoid scale.There is the decorative pattern like worm line shape body both sides, and both sides, body back are yellow black, and belly look shallow is in yellow-white, and build and body colour are extremely beautiful.The back of the body has continued hard sour jujube 11 pieces, more than be 13 pieces of bars that continue, pectoral fin fin ray 19 pieces, the hard sour jujube of abdomeinal fin 1 piece, continue bar 5 pieces, and anal fin has hard sour jujube 3 pieces, fin ray 13 pieces.The tail end likeness in form that continues is cut circular, and gill raker is sparse, and the flourishing tool pyloric caecum of digestive system stomach, enteron aisle is short, belongs to typical carnivorous type.
Current worm line river blackfish is in cultivating process, due to continuous fertilising, bait throwing in, the most destructible water quality of remaining excrement slag, residual bait, this just often causes worm line river blackfish to produce body surface ulcer, people are when processing fish disease, often according to the method that the state of an illness adopts disinfection sanitizer to splash, but medicine more or less all can have side effects or toxicity, although the pathogen in worm line river blackfish body surface and water can be killed, also usually cause the pollution of water body, and Long-Time Service can make medicine produce accumulation, affect body health, and then affect the growth rate of worm line river blackfish.Worm line river blackfish in growth course also Chang Yinwei subalimentation cause resistance not enough, cause body surface ulcer, affect the survival rate of worm line river blackfish, therefore, develop a kind of antibacterial ability that can improve and not only can ensure feed quality but also be beneficial to the worm line river blackfish mixed feed that worm line river blackfish grows to be the problem needing solution in current worm line river blackfish aquaculture industry badly.
Summary of the invention
An object of the present invention is to provide a kind of feed providing a kind of pre-insect protected line river blackfish body surface ulcer according to worm line river blackfish energetic supersession pattern and nutritional need, after its feeding worm line river blackfish, worm line river blackfish feed coefficient is low, specific growth rate is high, body surface ulcer recovers good, and anti-disease ability is strong.
In order to solve the problems of the technologies described above, the present invention by the following technical solutions, a kind of feed of pre-insect protected line river blackfish body surface ulcer, comprise each component of following masses number: fish meal 20-30 part, earthworm powder 8-15 part, squid powder 4-10 part, rice bran 6-12 part, saururus chinensis 3-7 part, water melon frost 3-8 part, snake slough 2-6 part, gold boils careless 3-7 part, cuttle bone 2-5 part, pollen pini 2-6 part, concha meretricis seu cylinae 1-5 part, calamine 1-4 part, granatum 2-6 part, sealwort 1-4 part, olive oil 1-4 part, compound vitamin 1-4 part.
Preferably, the feed of described pre-insect protected line river blackfish body surface ulcer, comprises each component of following masses number: fish meal 23-27 part, earthworm powder 10-13 part, squid powder 6-8 part, rice bran 8-10 part, saururus chinensis 4-6 part, water melon frost 4-7 part, snake slough 3-5 part, gold boils careless 4-6 part, cuttle bone 3-4 part, pollen pini 3-5 part, concha meretricis seu cylinae 2-4 part, calamine 2-3 part, granatum 3-5 part, sealwort 2-3 part, olive oil 2-3 part, compound vitamin 2-3 part.
Preferably, the feed of described pre-insect protected line river blackfish body surface ulcer, comprises each component of following masses number: fish meal 25 parts, earthworm powder 11 parts, squid powder 7 parts, 9 parts, rice bran, saururus chinensis 5 parts, water melon frost 6 parts, snake slough 4 parts, gold boils careless 5 parts, cuttle bone 3 parts, pollen pini 3 parts, concha meretricis seu cylinae 4 parts, calamine 3 parts, granatum 4 parts, sealwort 2 parts, 3 parts, olive oil, compound vitamin 3 parts.
The present invention is the feed of a kind of pre-insect protected line river blackfish body surface ulcer targetedly developed in abundant research worm line river blackfish digestive system structure and unique physiological basis of feeding and nutrition, can meet the nutritional need to protein and fat of worm line river blackfish.The present invention adopts saururus chinensis, water melon frost, snake slough, and gold boils grass, and cuttle bone, pollen pini, concha meretricis seu cylinae, calamine, granatum, sealwort is the ability that can improve worm line river blackfish postlarva resistance to surface ulcer, prevent disease.
Saururus chinensis taste is sweet, pungent, cold in nature, has clearing heat and detoxicating, effect of inducing diuresis to reduce edema, for difficult urination, drenches the puckery pain of drop, leukorrhea, urinary tract infections, nephritic dropsy; Control sore swollen toxin outward, eczema, saururus chinensis 50% decoction has inhibitory action to staphylococcus aureus, typhoid bacillus.Water melon frost taste is salty, cold in nature, has clearing heat-fire, effect of swelling and pain relieving.Snake slough taste is salty, sweet, and property is put down, and has and dispels the wind, and arresting convulsion, moves back screen, the effect of removing toxic substances, and for child convulsion, tic spasm, ocular, larynx numbness, treats swollen, pruitus, and scientific experiment proves that snake slough has the effect of good anti-inflammation.Gold boils careless bitter, pungent, salty, warm in nature, has sending down abnormally ascending, dissolving phlegm, effect of row water, and for cough due to wind-cold evil, recurrent fluid retention syndrome asthma due to excessive phlegm, distending pain in the hypochondrium, can pyogenic infections from tumour or sore, and rheumatalgia pain, has inhibitory action to staphylococcus aureus, streptococcus pneumonia, Pseudomonas aeruginosa, Escherichia coli.Cuttle bone taste is salty, puckery, warm in nature, astringing to arrest bleeding, treating spontaneous emission and leukorrhagia, relieving gastric hyperacidity to alleviate stomachache, hygroscopic sore, for canker, hyperhydrochloria, hematemesis and epistaxis, uterine bleeding is had blood in stool, emission and spermatorrhea, leukorrhea with reddish discharge, stomachache acid regurgitation, control bleeding due to trauma outward, the many pus of sore, scientific experiment proves that cuttle bone has good friction and suction-operated, not only can destroy the conjunctival epithelium tissue of pathology, the blood of toxin and bacterium may be adsorbed with again and ooze out mucus, but itself is without antibacterial action.Pollen pini taste is sweet, warm in nature, has the beneficial gas that dispels the wind, hygroscopic, effect of hemostasis, and control head and revolve dizzy, middle void has a stomach-ache, protracted dysentery, and all sores wet rotten, traumatic bleeding, effectively can protect skin, promote wound healing, and have immunization.Concha meretricis seu cylinae has effect of clearing away heat and promoting diuresis, and control hot asthma due to excessive phlegm and cough, oedema, gonorrhoea, goitre, knurl, gather, blood stasis in the chest pain, bloody flux, hemorrhoid, uterine bleeding, and under band, concha meretricis seu cylinae has the merit that relieving haperacidity relieves the pain, and concha meretricis seu cylinae and cuttle bone share, and its effect strengthens the effect that ulcer is recovered.Calamine taste is sweet, property is put down, and has removing toxic substances removing nebula, effect of the antipruritic sore of hygroscopic, be usually used in red eye, swell pain, blepharitis ciliaris, pterygium, pterygium, unhealing ulcer, pus is dripping, and wet sore itch, calamine has bactericidal action to pathogenic bacteria such as staphylococcus aureus, candida albicans, Escherichia coli.Granatum taste is sour, puckery, warm in nature, has relieving diarrhea with astringents, hemostasis, the effect of expelling parasite, and for rushing down for a long time, protracted dysentery, has blood in stool, prolapse of the anus, uterine bleeding, under band, and abdominal pain due to enterositosis.Containing multiple alkaloid in granatum, bacteriostatic test confirms that granatum has broad-spectrum antibacterial action, and it is to staphylococcus aureus, hemolytic streptococcus, have obvious inhibitory action addicted to aqueous vapor pseudomonas bacillus, shigella dysenteriae etc.Sealwort has boosting qi and nourishing yin, invigorating the spleen, moistening lung, effect of kidney-nourishing, be usually used in deficiency of spleen-QI and stomach-QI, fatigue and asthenia, deficiency of stomach-Yin, dry food is few, deficiency syndrome of the lung cough caused by dryness, labor is coughed hemoptysis, asthenia of essence and blood, sealwort water extraction fluid has inhibitory action to typhoid bacillus, staphylococcus aureus, acid-fast bacilli, and 2% sealwort has inhibitory action in various degree to common causative fungi in sabouraud culture medium.Saururus chinensis, water melon frost, snake slough, gold boils grass, cuttle bone, pollen pini, concha meretricis seu cylinae, calamine, granatum, sealwort acts synergistically each other, there is the effect of good suppression vibrios, and granatum, pollen pini, while other raw materials auxiliary play treatment ulcer, effectively can promote that fish is to the absorption of nutriment, improving the growth rate of worm line river blackfish fish.
Another object of the present invention is to provide a kind of preparation method of feed of above-mentioned pre-insect protected line river blackfish body surface ulcer, and the preparation method of the feed of this pre-insect protected line river blackfish body surface ulcer comprises the following steps:
(1) respectively will purchased from market fish meal, earthworm powder, squid powder, rice bran, and after compound vitamin takes mixing according to proportioning, load ball mill grinding 2h, cross 80 mesh sieves, obtain grounds travel;
(2) saururus chinensis is taken according to proportioning, water melon frost, snake slough, gold boils grass, cuttle bone, pollen pini, concha meretricis seu cylinae, calamine, granatum, sealwort is cleaned in pure fresh water, cleaner with distilled water flushing, add after purified water is heated to boiling and continue to boil 1.5h, obtain decoction liquor, decoction liquor is positioned in the extraction kettle in supercritical extract instrument, utilize carbon dioxide to extract as medium, extraction kettle pressure is 40Mpa, extraction temperature is 45 DEG C, separator pressure 12Mpa, and separator temperature is 70 DEG C, extract 2 hours, be extracted liquid;
(3) extract step (2) obtained concentrates at outer circulation inspissator, and concentrated condition is: pressure 0.03Mpa, temperature 70 C, is concentrated into the medicinal extract that relative density is 1.13 ~ 1.14;
(4) take according to proportioning grounds travel that olive oil and above-mentioned steps obtain, medicinal extract puts into container and be uniformly mixed and obtain object.
In preparation method, fish meal, earthworm powder, squid powder, rice bran, and compound vitamin is after ball milling, raw material Middle nutrition material is more easily separated out, liposoluble substance in raw material is more easily dissolved by the olive oil in subsequent step, adds the absorptivity of fish to the nutrition goods and materials of feed; By saururus chinensis, water melon frost, snake slough, gold boils grass, cuttle bone, pollen pini, concha meretricis seu cylinae, calamine, granatum, sealwort carries out boiling can the irritation effect of feed change, relatively mildly acts on fish, and supercritical extract, concentration step retain the nutritional labeling of raw material of the present invention to greatest extent.
The advantage that mash feed directly throws something and feeds made by the feed of the present invention's pre-insect protected line river blackfish body surface ulcer: preparation process is simple, and the feedstuff lost in preparation process is few, and the utilization rate of worm line river blackfish to feed is high.
Mixed feed of the present invention storage preservation condition: namely join namely use or, if the resting period was more than 20 days, 4 DEG C of sealings are preserved, if the resting period more than 3 months, puts ultra low temperature freezer preserve (i.e. subzero 80 degrees Celsius of preservations).
Compared with prior art, the invention has the beneficial effects as follows:
(1) worm line river blackfish likes feed of the present invention of ingesting, and feed of the present invention meets the nutritional need of worm line river blackfish; Invention adopts saururus chinensis, water melon frost, snake slough, and gold boils grass, cuttle bone, pollen pini, concha meretricis seu cylinae, calamine, granatum, sealwort for worm line river blackfish antibacterial ability can be improved, prevent disease;
Saururus chinensis taste is sweet, pungent, cold in nature, has clearing heat and detoxicating, effect of inducing diuresis to reduce edema, for difficult urination, drenches the puckery pain of drop, leukorrhea, urinary tract infections, nephritic dropsy; Control sore swollen toxin outward, eczema, saururus chinensis 50% decoction has inhibitory action to staphylococcus aureus, typhoid bacillus.Water melon frost taste is salty, cold in nature, has clearing heat-fire, effect of swelling and pain relieving.Snake slough taste is salty, sweet, and property is put down, and has and dispels the wind, and arresting convulsion, moves back screen, the effect of removing toxic substances, and for child convulsion, tic spasm, ocular, larynx numbness, treats swollen, pruitus, and scientific experiment proves that snake slough has the effect of good anti-inflammation.Gold boils careless bitter, pungent, salty, warm in nature, has sending down abnormally ascending, dissolving phlegm, effect of row water, and for cough due to wind-cold evil, recurrent fluid retention syndrome asthma due to excessive phlegm, distending pain in the hypochondrium, can pyogenic infections from tumour or sore, and rheumatalgia pain, has inhibitory action to staphylococcus aureus, streptococcus pneumonia, Pseudomonas aeruginosa, Escherichia coli.Cuttle bone taste is salty, puckery, warm in nature, astringing to arrest bleeding, treating spontaneous emission and leukorrhagia, relieving gastric hyperacidity to alleviate stomachache, hygroscopic sore, for canker, hyperhydrochloria, hematemesis and epistaxis, uterine bleeding is had blood in stool, emission and spermatorrhea, leukorrhea with reddish discharge, stomachache acid regurgitation, control bleeding due to trauma outward, the many pus of sore, scientific experiment proves that cuttle bone has good friction and suction-operated, not only can destroy the conjunctival epithelium tissue of pathology, the blood of toxin and bacterium may be adsorbed with again and ooze out mucus, but itself is without antibacterial action.Pollen pini taste is sweet, warm in nature, has the beneficial gas that dispels the wind, hygroscopic, effect of hemostasis, and control head and revolve dizzy, middle void has a stomach-ache, protracted dysentery, and all sores wet rotten, traumatic bleeding, effectively can protect skin, promote wound healing, and have immunization.Concha meretricis seu cylinae has effect of clearing away heat and promoting diuresis, and control hot asthma due to excessive phlegm and cough, oedema, gonorrhoea, goitre, knurl, gather, blood stasis in the chest pain, bloody flux, hemorrhoid, uterine bleeding, and under band, concha meretricis seu cylinae has the merit that relieving haperacidity relieves the pain, and concha meretricis seu cylinae and cuttle bone share, and its effect strengthens the effect that ulcer is recovered.Calamine taste is sweet, property is put down, and has removing toxic substances removing nebula, effect of the antipruritic sore of hygroscopic, be usually used in red eye, swell pain, blepharitis ciliaris, pterygium, pterygium, unhealing ulcer, pus is dripping, and wet sore itch, calamine has bactericidal action to pathogenic bacteria such as staphylococcus aureus, candida albicans, Escherichia coli.Granatum taste is sour, puckery, warm in nature, has relieving diarrhea with astringents, hemostasis, the effect of expelling parasite, and for rushing down for a long time, protracted dysentery, has blood in stool, prolapse of the anus, uterine bleeding, under band, and abdominal pain due to enterositosis.Containing multiple alkaloid in granatum, bacteriostatic test confirms that granatum has broad-spectrum antibacterial action, and it is to staphylococcus aureus, hemolytic streptococcus, have obvious inhibitory action addicted to aqueous vapor pseudomonas bacillus, shigella dysenteriae etc.Sealwort has boosting qi and nourishing yin, invigorating the spleen, moistening lung, effect of kidney-nourishing, be usually used in deficiency of spleen-QI and stomach-QI, fatigue and asthenia, deficiency of stomach-Yin, dry food is few, deficiency syndrome of the lung cough caused by dryness, labor is coughed hemoptysis, asthenia of essence and blood, sealwort water extraction fluid is to typhoid bacillus, staphylococcus aureus, acid-fast bacilli has inhibitory action, 2% sealwort has inhibitory action in various degree to common causative fungi in sabouraud culture medium, saururus chinensis, water melon frost, snake slough, gold boils grass, cuttle bone, pollen pini, concha meretricis seu cylinae, calamine, granatum, sealwort acts synergistically each other, there is the effect of good suppression vibrios, and granatum, pollen pini is while other raw materials auxiliary play treatment ulcer, effectively can promote that fish is to the absorption of nutriment, improve the growth rate of worm line river blackfish fish,
(2) each component that the feed of a kind of pre-insect protected line river blackfish body surface ulcer of the present invention is used is environmental protection raw material, health environment-friendly;
(3) in preparation method, fish meal, earthworm powder, squid powder, rice bran, and compound vitamin is after ball milling, raw material Middle nutrition material is more easily separated out, liposoluble substance in raw material is more easily dissolved by the olive oil in subsequent step, adds the absorptivity of fish to the nutrition goods and materials of feed; By saururus chinensis, water melon frost, snake slough, gold boils grass, cuttle bone, pollen pini, concha meretricis seu cylinae, calamine, granatum, sealwort carries out boiling can the irritation effect of feed change, relatively mildly acts on fish, and supercritical extract, concentration step retain the nutritional labeling of raw material of the present invention to greatest extent;
(4) feed of a kind of pre-insect protected line river blackfish body surface ulcer of the present invention is made mash feed and is is directly thrown something and fed, and preparation process is simple, and the feedstuff lost in preparation process is few, and the utilization rate of worm line river blackfish to feed is high.
Detailed description of the invention
Following embodiment is convenient to understand the present invention better, but does not limit the present invention.Experimental technique in following embodiment, if no special instructions, is conventional method.
Embodiment 1
In the present invention's first detailed description of the invention, feed ingredient and preparation method thereof is as follows:
A feed for pre-insect protected line river blackfish body surface ulcer, is made up of each component of following masses number: fish meal 20 parts, earthworm powder 8 parts, squid powder 4 parts, 6 parts, rice bran, saururus chinensis 3 parts, water melon frost 3 parts, snake slough 2 parts, gold boils careless 3 parts, cuttle bone 2 parts, pollen pini 2 parts, concha meretricis seu cylinae 1 part, calamine 1 part, granatum 2 parts, sealwort 1 part, 1 part, olive oil, compound vitamin 1 part.
The preparation method of the feed of above-mentioned a kind of pre-insect protected line river blackfish body surface ulcer is as follows:
(1) respectively will purchased from market fish meal, earthworm powder, squid powder, rice bran, and after compound vitamin takes mixing according to proportioning, load ball mill grinding 2h, cross 80 mesh sieves, obtain grounds travel;
(2) saururus chinensis is taken according to proportioning, water melon frost, snake slough, gold boils grass, cuttle bone, pollen pini, concha meretricis seu cylinae, calamine, granatum, sealwort is cleaned in pure fresh water, cleaner with distilled water flushing, add after purified water is heated to boiling and continue to boil 1.5h, obtain decoction liquor, decoction liquor is positioned in the extraction kettle in supercritical extract instrument, utilize carbon dioxide to extract as medium, extraction kettle pressure is 40Mpa, extraction temperature is 45 DEG C, separator pressure 12Mpa, and separator temperature is 70 DEG C, extract 2 hours, be extracted liquid;
(3) extract step (2) obtained concentrates at outer circulation inspissator, and concentrated condition is: pressure 0.03Mpa, temperature 70 C, is concentrated into the medicinal extract that relative density is 1.13 ~ 1.14;
(4) take according to proportioning grounds travel that olive oil and above-mentioned steps obtain, medicinal extract puts into container and be uniformly mixed and obtain object.
Embodiment 2
In the present invention's second detailed description of the invention, feed ingredient and preparation method thereof is as follows:
A feed for pre-insect protected line river blackfish body surface ulcer, is made up of each component of following masses number: fish meal 30 parts, earthworm powder 15 parts, squid powder 10 parts, 12 parts, rice bran, saururus chinensis 7 parts, water melon frost 8 parts, snake slough 6 parts, gold boils careless 7 parts, cuttle bone 5 parts, pollen pini 6 parts, concha meretricis seu cylinae 5 parts, calamine 4 parts, granatum 6 parts, sealwort 4 parts, 4 parts, olive oil, compound vitamin 4 parts.
The preparation method of the feed of above-mentioned pre-insect protected line river blackfish body surface ulcer is with embodiment 1.
Embodiment 3
In the present invention's the 3rd detailed description of the invention, feed ingredient and preparation method thereof is as follows:
A feed for pre-insect protected line river blackfish body surface ulcer, is made up of each component of following masses number: fish meal 23 parts, earthworm powder 10 parts, squid powder 6 parts, 8 parts, rice bran, saururus chinensis 4 parts, water melon frost 4 parts, snake slough 3 parts, gold boils careless 4 parts, cuttle bone 3 parts, pollen pini 3 parts, concha meretricis seu cylinae 2 parts, calamine 2 parts, granatum 3 parts, sealwort 2 parts, 2 parts, olive oil, compound vitamin 2 parts.
The preparation method of the feed of above-mentioned pre-insect protected line river blackfish body surface ulcer is with embodiment 1.
Embodiment 4
In the present invention's the 4th detailed description of the invention, feed ingredient and preparation method thereof is as follows:
A feed for pre-insect protected line river blackfish body surface ulcer, is made up of each component of following masses number: fish meal 27 parts, earthworm powder 13 parts, squid powder 8 parts, 10 parts, rice bran, saururus chinensis 6 parts, water melon frost 7 parts, snake slough 5 parts, gold boils careless 6 parts, cuttle bone 4 parts, pollen pini 5 parts, concha meretricis seu cylinae 4 parts, calamine 3 parts, granatum 5 parts, sealwort 3 parts, 3 parts, olive oil, compound vitamin 3 parts.
The preparation method of the feed of above-mentioned pre-insect protected line river blackfish body surface ulcer is with embodiment 1.
Embodiment 5
In the present invention's the 5th detailed description of the invention, feed ingredient and preparation method thereof is as follows:
A feed for pre-insect protected line river blackfish body surface ulcer, is made up of each component of following masses number: fish meal 25 parts, earthworm powder 11 parts, squid powder 7 parts, 9 parts, rice bran, saururus chinensis 5 parts, water melon frost 6 parts, snake slough 4 parts, gold boils careless 5 parts, cuttle bone 3 parts, pollen pini 3 parts, concha meretricis seu cylinae 4 parts, calamine 3 parts, granatum 4 parts, sealwort 2 parts, 3 parts, olive oil, compound vitamin 3 parts.
The preparation method of the feed of above-mentioned pre-insect protected line river blackfish body surface ulcer is with embodiment 1.
1, feeding experiment
Commercially available mixed feed (the commercially available mixed feed chosen in test is purchased from Shandong and rises rope fish feed research center) and the feed of embodiment of the present invention 1-embodiment 5 are carried out contrast experiment, and experimental result is as table 1-table 3:
To put into cement culturing pool with a collection of worm line river blackfish fish, each culturing pool is put into 1000 tails and is often planted feed process and establish four repetitions, and in this experimental result, data represent with mean+SD (mean ± SD).
Specific growth rate measures: measure the original body mass through hunger emptying worm line river blackfish fish when experiment starts, carry out daily feeding and management in experimentation, and experiment terminates the rear worm line river blackfish fish body weight measuring hungry two days.
Specific growth rate (SGR) formula of worm line river blackfish fish is:
SGR (part d
-1)=100 (lnWW2-lnWW1) T
-1;
WW1 and WW2 is respectively the initial and whole opisthosoma weight of worm line river blackfish fish in each cement culturing pool; T is experimental period (60 days).
From table 1, through 60d experiment, feed of the present invention compared with existing mixed feed, the specific growth rate significant difference (p<0.05) of worm line river blackfish fish.
The specific growth rate of table 1 DIFFERENT FEED processed group worm line river blackfish fish
As table 2, feed of the present invention is compared with existing mixed feed, and the survival rate of worm line river blackfish fish is without significant difference (p>0.05).
The survival rate of table 2 DIFFERENT FEED processed group worm line river blackfish fish
As table 3, feed of the present invention compared with existing mixed feed, the feed coefficient significant difference (p<0.05) of worm line river blackfish fish.
The feed coefficient of table 3 DIFFERENT FEED processed group worm line river blackfish fish
2, bacteriostatic test inspection
Adopt sodium dichloro cyanurate to contrast, embodiment 1 gained fish meal is that experimental group carries out antibacterial inspection.
The preparation of culture medium: take in the water-soluble 800mL of beef extract 5g, adds peptone 10g and dissolves, and adds casein 34g and dissolves, then adds NaCl5g dissolving, is adjusted to pH7.6 ~ 7.8, is diluted to 1000mL.Add agar powder 18g, autoclaving 15min, when being cooled to 40 DEG C ~ 50 DEG C, a kind of rhyme scheme in Chinese operas serving as the prelude to a complete score for voices, solidifies, and the 4 DEG C of refrigerators placing no bacteria pollution are for subsequent use.
Bacterial classification is: addicted to aqueous vapor pseudomonas bacillus.
Control group antibacterial liquid is prepared: be dissolved in respectively in 10ml aseptic deionized water by sodium dichloro cyanurate, diluted respectively by liquor strength, with stoste than being 1:2,1:4,1:8,1:16,1:32.
Experimental group antibacterial liquid is prepared: be dissolved in respectively in 10ml aseptic deionized water by fish meal leachate, diluted respectively by liquor strength, with stoste than being 1:2,1:4,1:8,1:16,1:32.The results are shown in Table 4 addicted to aqueous vapor pseudomonas bacillus is antibacterial.
Table 4
As can be seen from table 1-table 3, use the specific growth rate of the worm line river blackfish fish of feed of the present invention to be significantly higher than the specific growth rate (p<0.05) using existing mixed feed, the growth using feed of the present invention to be more conducive to worm line river blackfish fish is described; Use the survival rate of the worm line river blackfish fish of feed of the present invention and use the survival rate of existing mixed feed without significant difference (p>0.05); Feed coefficient significantly lower than the feed coefficient (p<0.05) using existing mixed feed, illustrates that, compared with existing mixed feed, feed conversion rate of the present invention is high, result of use good.
As can be seen from Table 4, fish meal of the present invention has better bactericidal effect.
The above is only the preferred embodiment of the present invention, is noted that for those skilled in the art, and under the premise without departing from the principles of the invention, can also make some improvement, these improvement should be considered as protection scope of the present invention.
Claims (4)
1. a feed for pre-insect protected line river blackfish body surface ulcer, is characterized in that, comprise each component of following masses number: fish meal 20-30 part, earthworm powder 8-15 part, squid powder 4-10 part, rice bran 6-12 part, saururus chinensis 3-7 part, water melon frost 3-8 part, snake slough 2-6 part, gold boils careless 3-7 part, cuttle bone 2-5 part, pollen pini 2-6 part, concha meretricis seu cylinae 1-5 part, calamine 1-4 part, granatum 2-6 part, sealwort 1-4 part, olive oil 1-4 part, compound vitamin 1-4 part.
2. the feed of a kind of pre-insect protected line river blackfish body surface ulcer according to claim 1, is characterized in that, comprise each component of following masses number: fish meal 23-27 part, earthworm powder 10-13 part, squid powder 6-8 part, rice bran 8-10 part, saururus chinensis 4-6 part, water melon frost 4-7 part, snake slough 3-5 part, gold boils careless 4-6 part, cuttle bone 3-4 part, pollen pini 3-5 part, concha meretricis seu cylinae 2-4 part, calamine 2-3 part, granatum 3-5 part, sealwort 2-3 part, olive oil 2-3 part, compound vitamin 2-3 part.
3. the feed of a kind of pre-insect protected line river blackfish body surface ulcer according to claim 1, is characterized in that, comprise each component of following masses number: fish meal 25 parts, earthworm powder 11 parts, squid powder 7 parts, 9 parts, rice bran, saururus chinensis 5 parts, water melon frost 6 parts, snake slough 4 parts, gold boils careless 5 parts, cuttle bone 3 parts, pollen pini 3 parts, concha meretricis seu cylinae 4 parts, calamine 3 parts, granatum 4 parts, sealwort 2 parts, 3 parts, olive oil, compound vitamin 3 parts.
4. a preparation method for the feed of a kind of pre-insect protected line river blackfish body surface ulcer that claim 1-3 is described arbitrarily, is characterized in that comprising the following steps:
(1) respectively will purchased from market fish meal, earthworm powder, squid powder, rice bran, and after compound vitamin takes mixing according to proportioning, load ball mill grinding 2h, cross 80 mesh sieves, obtain grounds travel;
(2) saururus chinensis is taken according to proportioning, water melon frost, snake slough, gold boils grass, cuttle bone, pollen pini, concha meretricis seu cylinae, calamine, granatum, sealwort is cleaned in pure fresh water, cleaner with distilled water flushing, add after purified water is heated to boiling and continue to boil 1.5h, obtain decoction liquor, decoction liquor is positioned in the extraction kettle in supercritical extract instrument, utilize carbon dioxide to extract as medium, extraction kettle pressure is 40Mpa, extraction temperature is 45 DEG C, separator pressure 12Mpa, and separator temperature is 70 DEG C, extract 2 hours, be extracted liquid;
(3) extract step (2) obtained concentrates at outer circulation inspissator, and concentrated condition is: pressure 0.03Mpa, temperature 70 C, is concentrated into the medicinal extract that relative density is 1.13 ~ 1.14;
(4) take according to proportioning grounds travel that olive oil and above-mentioned steps obtain, medicinal extract puts into container and be uniformly mixed and obtain object.
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| CN108185229A (en) * | 2018-03-28 | 2018-06-22 | 铜仁市万山区恒利达种养殖有限公司 | A kind of watermelon extractive from fermentative preparation method and applications for improving loach yield |
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