CN104774860A - 适于转化细胞的构建体、***及其应用 - Google Patents
适于转化细胞的构建体、***及其应用 Download PDFInfo
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- CN104774860A CN104774860A CN201410017168.3A CN201410017168A CN104774860A CN 104774860 A CN104774860 A CN 104774860A CN 201410017168 A CN201410017168 A CN 201410017168A CN 104774860 A CN104774860 A CN 104774860A
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Abstract
Description
基因特异位点 | 序列(5`-3`,SEQ ID NO:) |
CRY2-CRL1 | ACCGTGGCGAGAGTTCTTCTACAG(3) |
CRY2-CRR1 | AAAACTGTAGAAGAACTCTCGCCA(4) |
CRY2-CRL2 | ACCGTGGCCTGCTTCCTCACGCGG(5) |
CRY2-CRR2 | AAAACCGCGTGAGGAAGCAGGCCA(6) |
CRY2-CRL3 | ACCGCGGATTTCAGCGTGAATGCG(7) |
CRY2-CRR3 | AAAACGCATTCACGCTGAAATCCG(8) |
IL2RG-CRL1 | ACCGGGGGTGGGACTGAACCCGAG(9) |
IL2RG-CRR1 | AAAACTCGGGTTCAGTCCCACCCC(10) |
IL2RG-CRL2 | ACCGATGATAAAGTCCAGGAGTGG(11) |
IL2RG-CRR2 | AAAACCACTCCTGGACTTTATCAT(12) |
IL2RG-CRL3 | ACCGGCCACTATCTATTCTCTGAG(13) |
IL2RG-CRR3 | AAAACTCAGAGAATAGATAGTGGC(14) |
RAG1-CRL1 | ACCGAAAAGCAGGATTCCTCCGAG(15) |
RAG1-CRR1 | AAAACTCGGAGGAATCCTGCTTTT(16) |
RAG1-CRL2 | ACCGGAATTCTTTCAACACCACTG(17) |
RAG1-CRR2 | AAAACAGTGGTGTTGAAAGAATTC(18) |
RAG1-CRL3 | ACCGTAAAACCCAAGTCCTTTTAG(19) |
RAG1-CRR3 | AAAACTAAAAGGACTTGGGTTTTA(20) |
BRCA1-CRL1 | ACCGATTATTCTGAAGACCATTCG(21) |
BRCA1-CRR1 | AAAACGAATGGTCTTCAGAATAAT(22) |
BRCA1-CRL2 | ACCGGTCAGGGAAATAGGAGGTAG(23) |
BRCA1-CRR2 | AAAACTACCTCCTATTTCCCTGAC(24) |
BRCA1-CRL3 | ACCGGAGGTATGGTTTATGAGTAG(25) |
BRCA1-CRR3 | AAAACTACTCATAAACCATACCTC(26) |
BRCA1-CRL4 | ACCGTTTTAGTTCTTCTACCTAGG(27) |
BRCA1-CRR4 | AAAACCTAGGTAGAAGAACTAAAA(28) |
BRCA1-CRL5 | ACCGTACTTTGGTTTACTTTTAAG(29) |
BRCA1-CRR5 | AAAACTTAAAAGTAAACCAAAGTA(30) |
BRCA1-CRL6 | ACCGTTCTCATATCCAAAAATACG(31) |
BRCA1-CRR6 | AAAACGTATTTTTGGATATGAGAA(32) |
MSTN-CRL1 | ACCGCTTCAAAATCCACAGTTAGAG(33) |
MSTN-CRR1 | AAAACTCTAACTGTGGATTTTGAAG(34) |
MSTN-CRL2 | ACCGGATTTTGAAGCTTTTGGATGG(35) |
MSTN-CRR2 | AAAACCATCCAAAAGCTTCAAAATC(36) |
MSTN-CRL3 | ACCGGTGCACAAGATGAGTGTGAGG(37) |
MSTN-CRR3 | AAAACCTCACACTCATCTTGTGCAC(38) |
Claims (10)
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CN104774860A true CN104774860A (zh) | 2015-07-15 |
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
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CN112522309A (zh) * | 2020-09-04 | 2021-03-19 | 南京启真基因工程有限公司 | 重症免疫缺陷猪源重组细胞及其制备方法和试剂盒 |
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2014
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Non-Patent Citations (7)
Title |
---|
HERITABLE GENE-TARGETING WITH GRNA/CAS9 IN RATS: "Xinli Hu et al.", 《CELL RESEARCH》 * |
LE CONG ET AL.: "Multiplex Genome Engineering Using CRISPR/Cas Systems", 《SCIENCE》 * |
LEI S. QI ET AL.: "Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression", 《CELL》 * |
NANNAN CHANG ET AL.: "Genome editing with RNA-guided Cas9 nuclease in Zebrafish embryos", 《CELL RESEARCH》 * |
PRASHANT MALI ET AL.: "RNA-Guided Human Genome Engineering via Cas9", 《SCIENCE》 * |
SHU KONDO AND RYU UEDA: "Highly Improved Gene Targeting by Germline-Specific Cas9 Expression in Drosophila", 《GENETICS》 * |
WATARU FUJII ET AL.: "Efficient generation of large-scale genome-modified mice using gRNA and CAS9 endonuclease", 《NUCLEIC ACIDS RESEARCH》 * |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN112522309A (zh) * | 2020-09-04 | 2021-03-19 | 南京启真基因工程有限公司 | 重症免疫缺陷猪源重组细胞及其制备方法和试剂盒 |
CN112522309B (zh) * | 2020-09-04 | 2023-10-13 | 南京启真基因工程有限公司 | 重症免疫缺陷猪源重组细胞及其制备方法和试剂盒 |
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Address after: 518083 Yantian District, Yantian District, Shenzhen, Guangdong. Applicant after: BGI SHENZHEN Applicant after: Shenzhen Huada Sansheng Garden Technology Co.,Ltd. Address before: 518083 comprehensive building, Beishan Industrial Zone, Yantian District, Shenzhen, Guangdong Applicant before: BGI SHENZHEN Applicant before: SHENZHEN BGI AGRICULTURE AND CYCLE ECONOMIC TECHNOLOGY Co.,Ltd. |
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