CN104222680B - Loach specific complex immunostimulant and its preparation method - Google Patents

Loach specific complex immunostimulant and its preparation method Download PDF

Info

Publication number
CN104222680B
CN104222680B CN201410517625.5A CN201410517625A CN104222680B CN 104222680 B CN104222680 B CN 104222680B CN 201410517625 A CN201410517625 A CN 201410517625A CN 104222680 B CN104222680 B CN 104222680B
Authority
CN
China
Prior art keywords
extract
loach
parts
garlic
preparation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CN201410517625.5A
Other languages
Chinese (zh)
Other versions
CN104222680A (en
Inventor
闻芊倩
余妮泽
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Zhejiang Ocean University ZJOU
Original Assignee
Zhejiang Ocean University ZJOU
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Zhejiang Ocean University ZJOU filed Critical Zhejiang Ocean University ZJOU
Priority to CN201410517625.5A priority Critical patent/CN104222680B/en
Publication of CN104222680A publication Critical patent/CN104222680A/en
Application granted granted Critical
Publication of CN104222680B publication Critical patent/CN104222680B/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81—Aquaculture, e.g. of fish
    • Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
    • Y02P60/87—Re-use of by-products of food processing for fodder production

Landscapes

  • Fodder In General (AREA)
  • Cosmetics (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Medicines Containing Plant Substances (AREA)

Abstract

The present invention discloses loach specific complex immunostimulant and its preparation method, and this composite immune reinforcing agent is made up of the component of following weight part number: garlic 5-10 part, Radix Et Rhizoma Rhei extract 5-15 part, Fructus Forsythiae extract 1-5 part, hickory nut epicarp crude extract 1-5 part, Folium Lauri nobilis 4-6 part, Cortex Meliae 2-3 part, Folium Populi Pseudo-simonii 1-5 part, Herba Spirodelae extract 5-10 part, hawthorn 10-15 part, salt 10-20 part. The present invention as additive and conventional feed with the use of, loach immunity of organisms can be strengthened, it is to increase resistibility; Having no side effect and untoward reaction, the palatability for feed has no adverse effects, garlic, Radix Et Rhizoma Rhei extract, Fructus Forsythiae extract, hickory nut epicarp runic thing and salt with the use of, can annual prevention of water mildew, reduce sickness rate; Wheel worm and Gyrodactylus can be played the effect killed by Folium Lauri nobilis, Cortex Meliae, Folium Populi Pseudo-simonii; Prevention erythroderma is had good effect by Herba Spirodelae extract.

Description

Loach specific complex immunostimulant and its preparation method
Technical field
The invention belongs to field of feed additive technology, it is specifically related to a kind of loach specific complex immunostimulant and its preparation method.
Background technology
Loach, another name fish loach, body is elongated, and front section slightly cylindrical. Rear portion is flat-sided, belly circle, little. Young, the next, the shape of a hoof. Eye is little, without stinging now. Palpus 5. Squama is extremely tiny, circular, is embedded in subcutaneous. Body back and both sides grey black, entirety has many little black patchess, and head and each fin also have many black splotches, and the spot arrangement on dorsal fin and tail fin film is embarked on journey, and there is an obvious blackspot in caudal peduncle base portion. Loach is distributed widely in the ground such as China of Asia bank, Japan, Korea, Russia and India.
Mineral element and a large amount of VITAMIN such as loach rich in proteins, fat, carbohydrate and calcium, phosphorus, iron, wherein the content of VITMAIN B1 exceeds 3��4 times than fish, yellow croaker, shrimp, and the content of vitamin A, vitamins C and iron is also high than other fish. the light delicacy of loach meat, the delicious U.S. of meat, nutritious, and there is good dietary function. the market demand of loach increases in recent years, and supply falls short of demand. the export situation of loach is fine, and Japan, Korea S's demand are very big, and order increases year by year. therefore, natural fish for loach and far can not meet the need of market, loach is propagated artificially and starts to rise, often easily there is various disease and pest in breeding process in loach, especially annual three, April is easy to fish molds occur, loach common has wheel worm in the seed rearing stage, tongue cup worm, Gyrodactylus is parasitic and causes death, bubble illness and erythroderma are also that loach is in breeding process, it is easy to introduced disease, in prior art, for loach common disease and pest, normally chemical agent is adopted to kill again after finding disease and pest, instead of adopt the measure prevented in advance, this kind of chemical reagent is killed, slight disease and pest can be played certain effect, but for the disease and pest that the oncoming force is more violent, under normal circumstances, effect is not fine, the death of large quantities of loach can be caused, certain financial loss is brought to raiser, this kind of mode carries out killing of disease and pest, just play interim killing, can not fundamentally cure, the generation of disease and pest, cause the immunity degradation of loach, mortality ratio increases, seriously have impact on the economic benefit of the speed of growth of loach and cultivation, how to prevent before disease and pest occurs, improve the invasion of fish body opposing disease and pest, reduce sickness rate, become crucial. in the breeding process of loach, throwing something and feeding of feed is a step of most critical, and the raising of the resistibility of loach own, also closely related with throwing something and feeding of feed, therefore, feed adds composite immune reinforcing agent, to regulate body health, enhancing body immunizing power, restraining and sterilizing bacteria pest-resistant by feed of throwing something and feeding, propagating artificially of loach is extremely important.
Summary of the invention
For problems of the prior art, the object of the invention is at a kind of loach specific complex immunostimulant of offer and its preparation method.
The present invention is realized by the following technical programs:
Described loach specific complex immunostimulant, it is characterised in that this composite immune reinforcing agent is made up of the component of following weight part number: garlic 5-10 part, Radix Et Rhizoma Rhei extract 5-15 part, Fructus Forsythiae extract 1-5 part, hickory nut epicarp crude extract 1-5 part, Folium Lauri nobilis 4-6 part, Cortex Meliae 2-3 part, Folium Populi Pseudo-simonii 1-5 part, Herba Spirodelae extract 5-10 part, hawthorn 10-15 part, salt 10-20 part.
Described loach specific complex immunostimulant, it is characterised in that be made up of the component of following weight part number: 7 parts, garlic, Radix Et Rhizoma Rhei extract 10 parts, Fructus Forsythiae extract 3 parts, hickory nut epicarp crude extract 2 parts, Folium Lauri nobilis 5 parts, Cortex Meliae 3 parts, Folium Populi Pseudo-simonii 3 parts, Herba Spirodelae extract 8 parts, hawthorn 15 parts, salt 20 parts.
Described loach specific complex immunostimulant, it is characterised in that this composite immune reinforcing agent adds in loach mixed feed, the use ratio of composite immune reinforcing agent and loach mixed feed is 1:50-80.
Described loach specific complex immunostimulant, it is characterised in that described composite immune reinforcing agent adds in loach mixed feed, the use ratio of composite immune reinforcing agent and loach mixed feed is 1:65.
The preparation method of described loach specific complex immunostimulant, it is characterised in that follow these steps to carry out:
1) take the big Folium Lauri nobilis of formula ratio, Cortex Meliae, Folium Populi Pseudo-simonii, hawthorn respectively, clean clean after dry in drying baker, after drying, each composition is pulverized respectively, wears into fine powder, crosses 40 orders sieves, obtains each composition dry powder, for subsequent use;
2) the fresh garlic head after the peeling of formula ratio is got, with pulverizer, Bulbus Allii head is processed into mashed garlic, mashed garlic is put into baking oven, dry with slow fire, temperature controls at 65 DEG C, until mashed garlic becomes block, in drying course, stirred mashed garlic every 1 hour once, mashed garlic is heated evenly, mashed garlic after drying is pulverized in pulverizer, crosses 60 order sieves, obtain garlic powder;
3) by step 2) after the obtained each composition dry powder blend of obtained garlic powder, step 1), add the Radix Et Rhizoma Rhei extract of formula ratio, Fructus Forsythiae extract, hickory nut epicarp crude extract, Herba Spirodelae extract, salt mixing, stir evenly.
The preparation method of described loach specific complex immunostimulant, it is characterized in that the preparation method of described Radix Et Rhizoma Rhei extract is: rheum officinale is pulverized, cross 40 order sieves, by 90% ethanolic soln refluxing extraction, extract 1-2 hour every time, repeat to extract 2-3 time, the weightmeasurement ratio of rhubarb horsetails powder and ethanol is 1:15-20g/ml, extracting solution concentrates to medicinal extract, and medicinal extract spraying dry under the temperature condition of 165 DEG C, obtains Radix Et Rhizoma Rhei extract.
The preparation method of described loach specific complex immunostimulant, it is characterized in that the preparation method of described Fructus Forsythiae extract is: Folium Forsythiae is pulverized, cross 40 order sieves, the water of times weight that adds 15 refluxing extraction 2-3 hour, extracting liquid filtering, filtrate concentrates to medicinal extract, and medicinal extract spraying dry under the temperature condition of 165 DEG C, obtains Fructus Forsythiae extract.
The preparation method of described loach specific complex immunostimulant, it is characterized in that the preparation method of described hickory nut epicarp crude extract is: hickory nut epicarp is dried, pulverized, cross 40 order sieves, the alcohol steep adding 75% 48 hours, vat liquor concentrates to medicinal extract, medicinal extract spraying dry under the temperature condition of 170 DEG C, obtains hickory nut epicarp crude extract.
The preparation method of described loach specific complex immunostimulant, it is characterized in that the preparation method of described Herba Spirodelae extract is: duckweed is grass oven dry pulverizing entirely, adding 20 times of concentration by weight is the ethanolic soln of 90%, heating and refluxing extraction 1-3 time, each 1-1.5 hour, extracting solution merged, and concentrates to medicinal extract, medicinal extract spraying dry under the temperature condition of 170 DEG C, obtains Herba Spirodelae extract.
Garlic nutrient enriches: every 100 grams of moisture contents 69.8 grams, 4.4 grams, protein, 0.2 gram, fat, 23.6 grams, carbohydrate, calcium 5 milligrams, 44 milligrams, phosphorus, iron 0.4 milligram, vitamins C 3 milligrams. In addition, also containing VitB1, riboflavin, nicotinic acid, allicin, citral and the trace element such as selenium and germanium. Containing volatile oil about 0.2%, in oil, main component is allicin, and the alliin being contained in garlic is produced by the effect hydrolysis of big allinase. Still containing the sulfide compound etc. of multiple allyl group, propyl group and methyl composition. Garlic has powerful sterilizing ability, can eliminate the germ in intrusive body. It also helps the absorption of VITMAIN B1, promotes that the metabolism of carbohydrate is with generate energy, and alleviating fatigue. Garlic integrates more than 100 kind of medicine and health care composition, wherein containing sulphur volatile matter 43 kinds, and sulfuration-sulfinic acid (such as garlicin) ester class 13 kinds, 9 kinds, amino acid, peptide class 8 kinds, glucoside class 12 kinds, enzyme class 11 kinds. In addition, alliin is the unique composition of garlic, just becomes garlicin when it enters blood, even if this kind of garlicin dilution 100,000 times still can kill Corynebacterium diphtheriae, dysentery bacterium, influenza virus etc. instantaneously.
Rheum officinale, the capsule of weeping forsythia are medicinal plant, have effect of antisepsis and anti-inflammation, and both are composite, its antibiotic and sterilizing ability strengthens, rheum officinale, the capsule of weeping forsythia, hickory nut epicarp crude extract and garlic and salt are used in combination, can effectively prevent the generation of fish molds, also can improve the immunological competence of human body. The generation of loach wheel worm and Gyrodactylus can be effectively avoided together with Folium Lauri nobilis is composite with Cortex Meliae and Folium Populi Pseudo-simonii.
Duckweed nature and flavor are pungent, tremble with fear, return lung (gill), bladder warp. The effects such as tool is clearing heat and detoxicating, promoting eruption, inducing diuresis to remove edema. Grass is main containing orientin, Vitexin, apiose, Reseda odorata flavine-7-glycoside entirely for duckweed. Still containing Potassium ethanoate, Repone K, iodine, bromine, chlorophyll, tannin and lipoid cpd (linolenic acid, palmitinic acid, linolic acid) etc. Wherein, tannin is the phenolic compound of a class complexity, has astringent taste and convergency, can be combined with protein, mucus, alkaloid salt, heavy metallic salt and generate precipitation. Phenols has anti-microbial effect, and the deleterious effect of bacterium may mainly be made protein denaturation by it, has again the effect of tensio-active agent simultaneously, destroys membrane passage, makes cell content excessive. It is lethal gene when concentration height, otherwise then plays bacteriostatic action. Therefore it is conventional as astringing to arrest bleeding, antidiarrheal, antisepsis and anti-inflammation. Can be used as the toxinicide of alkaloid and heavy metal (be combined with heavy metal and generate coordination compound, and excrete) in addition. The solubility salt of chlorophyll has certain antibacterial and anti-inflammation functions and promotes the effect of granulation growth. Iodine is strong sterilant, can kill bacterium, fungi, and make inactivation of virus. Potassium ethanoate is strong base-weak acid salt, in alkalescence, can constantly neutralize the acid that bacterium produces, and highly basic is stronger than gram positive bacterium effect to gram negative bacterium and virus. The pathogenic agent of erythroderma is firefly light pole hair bacillus, is Gram-negative mattress. Therefore, duckweed not only can anti-fish evil myxococcus and the firefly light pole hair bacillus of erythroderma, and can be antiviral. In addition, duckweed is also containing the unsaturated fatty acids such as linolenic acid, palmitinic acid, linolic acid, and its Linoleic acid is the most important unsaturated fatty acidss of fish, can synthesis of prostaglandins.
Compared with existing preparation, the present invention has the following advantages:
1) as additive and loach conventional feed with the use of, garlic, Radix Et Rhizoma Rhei extract, Fructus Forsythiae extract, hickory nut epicarp runic thing and salt with the use of, can annual prevention of water mildew, reduce sickness rate;
2) Folium Lauri nobilis, Cortex Meliae, Folium Populi Pseudo-simonii and feed formulation use, and wheel worm and Gyrodactylus can play the effect killed;
3) interpolation of Herba Spirodelae extract, has good effect to prevention erythroderma;
4) cooperation of each composition, can strengthen loach immunity of organisms, it is to increase to the resistibility of disease and pest;
5) having no side effect and untoward reaction, the palatability for feed has no adverse effects, the interpolation of hawthorn, is convenient to improve the palatability of feed.
Embodiment
By following specific embodiment, the invention will be further described.
Embodiment 1
Loach specific complex immunostimulant, consists of: 7 parts, garlic, Radix Et Rhizoma Rhei extract 10 parts, Fructus Forsythiae extract 3 parts, hickory nut epicarp crude extract 2 parts, Folium Lauri nobilis 5 parts, Cortex Meliae 3 parts, Folium Populi Pseudo-simonii 3 parts, Herba Spirodelae extract 8 parts, hawthorn 15 parts, salt 20 parts. Its preparation method is: take the big Folium Lauri nobilis of formula ratio, Cortex Meliae, Folium Populi Pseudo-simonii, hawthorn respectively, clean clean after dry in drying baker, after drying, each composition is pulverized respectively, wears into fine powder, crosses 40 orders sieves, obtains each composition dry powder; Get the fresh garlic head after the peeling of formula ratio, with pulverizer, Bulbus Allii head is processed into mashed garlic, mashed garlic is put into baking oven, dry with slow fire, temperature controls at 65 DEG C, until mashed garlic becomes block, in drying course, stirred mashed garlic every 1 hour once, mashed garlic is heated evenly, mashed garlic after drying is pulverized in pulverizer, crosses 60 order sieves, obtain garlic powder; The Radix Et Rhizoma Rhei extract of each composition dry powder of above-mentioned preparation and garlic powder and formula ratio, Fructus Forsythiae extract, hickory nut epicarp crude extract, Herba Spirodelae extract, salt are mixed, stirs evenly i.e. obtained loach specific complex immunostimulant. Getting the loach specific complex immunostimulant 1 part prepared, the loach conventional feed with 65 parts mixes, and is thrown something and fed by loach.
Wherein: the preparation method of Radix Et Rhizoma Rhei extract is: rheum officinale is pulverized, cross 40 order sieves, by 90% ethanolic soln refluxing extraction, extract 1-2 hour every time, repeating to extract 2-3 time, the weightmeasurement ratio of rhubarb horsetails powder and ethanol is 1:15-20g/ml, and extracting solution concentrates to medicinal extract, medicinal extract spraying dry under the temperature condition of 165 DEG C, obtains Radix Et Rhizoma Rhei extract.
The preparation method of Fructus Forsythiae extract is: Folium Forsythiae is pulverized, and crosses 40 orders sieves, and the water of times weight that adds 15 refluxing extraction 2-3 hour, extracting liquid filtering, filtrate concentrates to medicinal extract, and medicinal extract spraying dry under the temperature condition of 165 DEG C, obtains Fructus Forsythiae extract.
The preparation method of hickory nut epicarp crude extract is: hickory nut epicarp is dried, pulverized, and crosses 40 order sieves, and the alcohol steep adding 75% 48 hours, vat liquor concentrates to medicinal extract, and medicinal extract spraying dry under the temperature condition of 170 DEG C, obtains hickory nut epicarp crude extract.
The preparation method of Herba Spirodelae extract is: duckweed is grass oven dry pulverizing entirely, and adding 20 times of concentration by weight is the ethanolic soln of 90%, heating and refluxing extraction 1-3 time, each 1-1.5 hour, extracting solution merged, and concentrates to medicinal extract, medicinal extract spraying dry under the temperature condition of 170 DEG C, obtains Herba Spirodelae extract.
Embodiment 2
Loach specific complex immunostimulant, consists of: 5 parts, garlic, Radix Et Rhizoma Rhei extract 15 parts, Fructus Forsythiae extract 1 part, hickory nut epicarp crude extract 5 parts, Folium Lauri nobilis 4 parts, Cortex Meliae 3 parts, Folium Populi Pseudo-simonii 1 part, Herba Spirodelae extract 10 parts, hawthorn 10 parts, salt 20 parts. Its preparation method is: take the big Folium Lauri nobilis of formula ratio, Cortex Meliae, Folium Populi Pseudo-simonii, hawthorn respectively, clean clean after dry in drying baker, after drying, each composition is pulverized respectively, wears into fine powder, crosses 40 orders sieves, obtains each composition dry powder; Get the fresh garlic head after the peeling of formula ratio, with pulverizer, Bulbus Allii head is processed into mashed garlic, mashed garlic is put into baking oven, dry with slow fire, temperature controls at 65 DEG C, until mashed garlic becomes block, in drying course, stirred mashed garlic every 1 hour once, mashed garlic is heated evenly, mashed garlic after drying is pulverized in pulverizer, crosses 60 order sieves, obtain garlic powder; The Radix Et Rhizoma Rhei extract of each composition dry powder of above-mentioned preparation and garlic powder and formula ratio, Fructus Forsythiae extract, hickory nut epicarp crude extract, Herba Spirodelae extract, salt are mixed, stirs evenly i.e. obtained loach specific complex immunostimulant. Getting the loach specific complex immunostimulant 1 part prepared, the loach conventional feed with 50 parts mixes, and is thrown something and fed by loach.
The extracting method of Radix Et Rhizoma Rhei extract, Fructus Forsythiae extract, hickory nut epicarp crude extract, Herba Spirodelae extract is with embodiment 1.
Embodiment 3
Loach specific complex immunostimulant, consists of: 10 parts, garlic, Radix Et Rhizoma Rhei extract 5 parts, Fructus Forsythiae extract 5 parts, hickory nut epicarp crude extract 1 part, Folium Lauri nobilis 6 parts, Cortex Meliae 2 parts, Folium Populi Pseudo-simonii 5 parts, Herba Spirodelae extract 5 parts, hawthorn 5 parts, salt 10 parts. Its preparation method is: take the big Folium Lauri nobilis of formula ratio, Cortex Meliae, Folium Populi Pseudo-simonii, hawthorn respectively, clean clean after dry in drying baker, after drying, each composition is pulverized respectively, wears into fine powder, crosses 40 orders sieves, obtains each composition dry powder; Get the fresh garlic head after the peeling of formula ratio, with pulverizer, Bulbus Allii head is processed into mashed garlic, mashed garlic is put into baking oven, dry with slow fire, temperature controls at 65 DEG C, until mashed garlic becomes block, in drying course, stirred mashed garlic every 1 hour once, mashed garlic is heated evenly, mashed garlic after drying is pulverized in pulverizer, crosses 60 order sieves, obtain garlic powder; The Radix Et Rhizoma Rhei extract of each composition dry powder of above-mentioned preparation and garlic powder and formula ratio, Fructus Forsythiae extract, hickory nut epicarp crude extract, Herba Spirodelae extract, salt are mixed, stirs evenly i.e. obtained loach specific complex immunostimulant. Getting the loach specific complex immunostimulant 1 part prepared, the loach conventional feed with 80 parts mixes, and is thrown something and fed by loach.
The extracting method of Radix Et Rhizoma Rhei extract, Fructus Forsythiae extract, hickory nut epicarp crude extract, Herba Spirodelae extract is with embodiment 1.
Control group 1
Loach specific complex immunostimulant, consists of: 4 parts, garlic, Radix Et Rhizoma Rhei extract 17 parts, Fructus Forsythiae extract 6 parts, hickory nut epicarp crude extract 0.5 part, Folium Lauri nobilis 2 parts, Cortex Meliae 5 parts, Folium Populi Pseudo-simonii 0.5 part, Herba Spirodelae extract 15 parts, hawthorn 8 parts, salt 5 parts. Preparation method is with embodiment 1. Getting loach specific complex immunostimulant 1 part, the loach conventional feed with 65 parts mixes, and is thrown something and fed by loach.
Control group 2
Adopt and throw something and feed with the conventional feed that embodiment 1 is identical, do not add composite immune reinforcing agent.
Test example
Pond is separated into five regions of the same area, it is numbered 1,2,3,4,5, in the beginning of June, throws in loach seedling 200 tail that body length is about 3cm respectively to each marker space, wherein, 1 throws something and feeds according to the formulation content of embodiment 1, and 2 throw something and feed according to the formulation content of embodiment 2, and 3 throw something and feed according to the formulation content of embodiment 3,4 throw something and feed according to the formulation content of control group, 5 throw something and feed according to control group 2 formulation content, feed 6 months, and feeding effect is in table 1.
Table 1 feeding effect
Experimental result shows: when ambient conditions is identical, and loach specific complex immunostimulant of the present invention can significantly improve rate of growth and the body weight of loach, reduces the sickness rate of loach, it is to increase the survival rate of loach, reduces feed coefficient. As seen from Table 1; the loach of No. 1 isolated area that employing embodiment 1 content formula is thrown something and fed; every index is all better than other each group; the loach of No. 4 isolated areas that employing control group 1 content formula is thrown something and fed, owing to the content of each composition of control group 1 is not all in the protection domain of present patent application, its every index fed is all lower than the feeding effect of embodiment 1-3; as can be seen from the table; its surviving rate is on the low side, is because the addition of Herba Spirodelae extract is on the high side, causes the dead quantity of loach fry to increase.

Claims (9)

1. loach specific complex immunostimulant, it is characterised in that this composite immune reinforcing agent is made up of the component of following weight part number: garlic 5-10 part, Radix Et Rhizoma Rhei extract 5-15 part, Fructus Forsythiae extract 1-5 part, hickory nut epicarp crude extract 1-5 part, Folium Lauri nobilis 4-6 part, Cortex Meliae 2-3 part, Folium Populi Pseudo-simonii 1-5 part, Herba Spirodelae extract 5-10 part, hawthorn 10-15 part, salt 10-20 part.
2. loach specific complex immunostimulant according to claim 1, it is characterised in that be made up of the component of following weight part number: 7 parts, garlic, Radix Et Rhizoma Rhei extract 10 parts, Fructus Forsythiae extract 3 parts, hickory nut epicarp crude extract 2 parts, Folium Lauri nobilis 5 parts, Cortex Meliae 3 parts, Folium Populi Pseudo-simonii 3 parts, Herba Spirodelae extract 8 parts, hawthorn 15 parts, salt 20 parts.
3. loach specific complex immunostimulant according to claim 1 and 2, it is characterised in that this composite immune reinforcing agent adds in loach mixed feed, and the use ratio of composite immune reinforcing agent and loach mixed feed is 1:50-80.
4. loach specific complex immunostimulant according to claim 3, it is characterised in that described composite immune reinforcing agent adds in loach mixed feed, and the use ratio of composite immune reinforcing agent and loach mixed feed is 1:65.
5. the preparation method of loach specific complex immunostimulant, it is characterised in that follow these steps to carry out:
1) take the big Folium Lauri nobilis of formula ratio, Cortex Meliae, Folium Populi Pseudo-simonii, hawthorn respectively, clean clean after dry in drying baker, after drying, each composition is pulverized respectively, wears into fine powder, crosses 40 orders sieves, obtains each composition dry powder, for subsequent use;
2) the fresh garlic head after the peeling of formula ratio is got, with pulverizer, Bulbus Allii head is processed into mashed garlic, mashed garlic is put into baking oven, dry with slow fire, temperature controls at 65 DEG C, until mashed garlic becomes block, in drying course, stirred mashed garlic every 1 hour once, mashed garlic is heated evenly, mashed garlic after drying is pulverized in pulverizer, crosses 60 order sieves, obtain garlic powder;
3) by step 2) after the obtained each composition dry powder blend of obtained garlic powder, step 1), add the Radix Et Rhizoma Rhei extract of formula ratio, Fructus Forsythiae extract, hickory nut epicarp crude extract, Herba Spirodelae extract, salt mixing, stir evenly.
6. the preparation method of loach specific complex immunostimulant according to claim 5, it is characterized in that the preparation method of described Radix Et Rhizoma Rhei extract is: rheum officinale is pulverized, cross 40 order sieves, by 90% ethanolic soln refluxing extraction, extract 1-2 hour every time, repeat to extract 2-3 time, the weightmeasurement ratio of rhubarb horsetails powder and ethanol is 1:15-20g/ml, extracting solution concentrates to medicinal extract, and medicinal extract spraying dry under the temperature condition of 165 DEG C, obtains Radix Et Rhizoma Rhei extract.
7. the preparation method of loach specific complex immunostimulant according to claim 5, it is characterized in that the preparation method of described Fructus Forsythiae extract is: Folium Forsythiae is pulverized, cross 40 order sieves, the water of times weight that adds 15 refluxing extraction 2-3 hour, extracting liquid filtering, filtrate concentrates to medicinal extract, and medicinal extract spraying dry under the temperature condition of 165 DEG C, obtains Fructus Forsythiae extract.
8. the preparation method of loach specific complex immunostimulant according to claim 5, it is characterized in that the preparation method of described hickory nut epicarp crude extract is: hickory nut epicarp is dried, pulverized, cross 40 order sieves, the alcohol steep adding 75% 48 hours, vat liquor concentrates to medicinal extract, medicinal extract spraying dry under the temperature condition of 170 DEG C, obtains hickory nut epicarp crude extract.
9. the preparation method of loach specific complex immunostimulant according to claim 5, it is characterized in that the preparation method of described Herba Spirodelae extract is: duckweed is grass oven dry pulverizing entirely, adding 20 times of concentration by weight is the ethanolic soln of 90%, heating and refluxing extraction 1-3 time, each 1-1.5 hour, extracting solution merged, and concentrates to medicinal extract, medicinal extract spraying dry under the temperature condition of 170 DEG C, obtains Herba Spirodelae extract.
CN201410517625.5A 2014-09-30 2014-09-30 Loach specific complex immunostimulant and its preparation method Expired - Fee Related CN104222680B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201410517625.5A CN104222680B (en) 2014-09-30 2014-09-30 Loach specific complex immunostimulant and its preparation method

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201410517625.5A CN104222680B (en) 2014-09-30 2014-09-30 Loach specific complex immunostimulant and its preparation method

Publications (2)

Publication Number Publication Date
CN104222680A CN104222680A (en) 2014-12-24
CN104222680B true CN104222680B (en) 2016-06-08

Family

ID=52212288

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201410517625.5A Expired - Fee Related CN104222680B (en) 2014-09-30 2014-09-30 Loach specific complex immunostimulant and its preparation method

Country Status (1)

Country Link
CN (1) CN104222680B (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105815601A (en) * 2016-03-21 2016-08-03 沈阳工学院 Immunopotentiator special for medaka and preparation method and application of immunopotentiator
CN106306385A (en) * 2016-08-19 2017-01-11 怀远县渔业科技发展有限责任公司 Attracting immunity-improving feed additive for oriental weatherfish and preparation method thereof
CN106260780A (en) * 2016-08-19 2017-01-04 怀远县渔业科技发展有限责任公司 A kind of feed additive for treating Misgurni anguillicaudati edema disease and preparation method thereof
CN106858061A (en) * 2017-03-24 2017-06-20 河南科高中标检测技术有限公司 A kind of application for the composition of natural products of additive for farm animal feed and its in feed addictive
CN108617887A (en) * 2018-05-11 2018-10-09 宿松县龙源水产养殖专业合作社 A kind of loach summer heat-clearing appetizer
CN108888699A (en) * 2018-07-25 2018-11-27 贵州贵丰农业发展有限公司 A method of prevention and treatment soft-shelled turtle saprolegniasis

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH08116887A (en) * 1994-10-27 1996-05-14 Solar Japan Kk Method for culturing loach fry
KR20000053916A (en) * 2000-05-08 2000-09-05 전효천 How to prepare Chueotang for removing fishy taste
CN1270622C (en) * 2005-01-11 2006-08-23 冯绍宽 Plant additive premixed forage for aquatic culture and bait therefrom
CN102125190B (en) * 2011-03-02 2012-09-05 江苏华威农牧发展有限公司 Loach feed bred in high-density and scaled mode
CN102293335A (en) * 2011-07-01 2011-12-28 广西粤海饲料有限公司 Universal composite Chinese herbal immunopotentiator for fish cultured in freshwater
CN103844145A (en) * 2014-03-12 2014-06-11 江苏天成科技集团有限公司 Method for producing novel loach feed
CN104041440A (en) * 2014-05-29 2014-09-17 郎溪县兴贵水产品养殖家庭农场 Raising method with function of facilitating growth of misgurnus anguillicaudatus

Also Published As

Publication number Publication date
CN104222680A (en) 2014-12-24

Similar Documents

Publication Publication Date Title
CN104222680B (en) Loach specific complex immunostimulant and its preparation method
CN103141710B (en) Compound feed for weaned pigs and preparation method of feed
CN102845638B (en) Duck feed capable of preventing and treating avian influenza and Chinese herbal additive used therein
CN103931686A (en) Plant pesticide as well as preparation method and application thereof
KR102213743B1 (en) A process and the composition for increasing immunity-boosting and the growth of animals comprising herbs
CN104095153A (en) Special-purpose immunopotentiating agent for dungat groupers
CN102919607B (en) Chinese herbal medicine compound feed additive with anti-diarrhea function and improving pork quality
CN104206914B (en) A kind of manufacture method of Rrinaceus earopaeus formula forage
KR101737271B1 (en) Natural extract containing a large amount of Omega-3 and Manufacturing method thereof
Sorwar et al. Effect of kalo jeera seeds and papaya leaf supplementation on the performance of broiler.
CN105815611A (en) Chinese herbal attractant for aquatic animals
KR20020032807A (en) Meat of Duck Fed on Garlic and/or Oriental Medicines and Method of Feeding the Duck
CN106578484A (en) Chinese herbal medicine feed supplement for sows in suckling period
KR20120095505A (en) Foot-and-mouth disease prevention useful feed and method of preparing thereof
RU2641907C1 (en) Bioactive fodder additive for agricultural animals and poultry
CN103918935A (en) Pet food additive
CN107296173A (en) Improve aquatic livestock immunity feed and preparation method thereof
Bottoni et al. Alpine ethnobotanical knowledge in Sondalo (SO, Lombardy, Italy)
CN106359203A (en) Method for improving quality of fish
KR100466946B1 (en) Feed for Duck and Method of Feeding Duck with the Feed
CN105341534A (en) Feed additive for preventing white-spot disease of parrot fishes and preparation method thereof
Amad et al. Moringa (M. oleifera) leaf meal in diets for broilers and laying hens: a review
Popović et al. Medicinal properties of buckwheat products and honey in compliance with food safety regulatory requirements
KR102582313B1 (en) Composition for improving joint health by reducing body fat in companion dogs
CN106614178A (en) Composite preparation for preventing and treating water retention disease of river crab and application thereof

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C14 Grant of patent or utility model
GR01 Patent grant
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20160608

Termination date: 20180930

CF01 Termination of patent right due to non-payment of annual fee