CN104222680B - Loach specific complex immunostimulant and its preparation method - Google Patents
Loach specific complex immunostimulant and its preparation method Download PDFInfo
- Publication number
- CN104222680B CN104222680B CN201410517625.5A CN201410517625A CN104222680B CN 104222680 B CN104222680 B CN 104222680B CN 201410517625 A CN201410517625 A CN 201410517625A CN 104222680 B CN104222680 B CN 104222680B
- Authority
- CN
- China
- Prior art keywords
- extract
- loach
- parts
- garlic
- preparation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 241000252185 Cobitidae Species 0.000 title claims abstract description 81
- 229960001438 immunostimulant agent Drugs 0.000 title claims abstract description 35
- 239000003022 immunostimulating agent Substances 0.000 title claims abstract description 35
- 230000003308 immunostimulating effect Effects 0.000 title claims abstract description 35
- 238000002360 preparation method Methods 0.000 title claims abstract description 35
- 239000000284 extract Substances 0.000 claims abstract description 100
- 240000002234 Allium sativum Species 0.000 claims abstract description 52
- 235000004611 garlic Nutrition 0.000 claims abstract description 52
- 241000899834 Obovaria olivaria Species 0.000 claims abstract description 28
- 239000000287 crude extract Substances 0.000 claims abstract description 23
- 150000003839 salts Chemical class 0.000 claims abstract description 20
- 235000009917 Crataegus X brevipes Nutrition 0.000 claims abstract description 15
- 235000013204 Crataegus X haemacarpa Nutrition 0.000 claims abstract description 15
- 235000009685 Crataegus X maligna Nutrition 0.000 claims abstract description 15
- 235000009444 Crataegus X rubrocarnea Nutrition 0.000 claims abstract description 15
- 235000009486 Crataegus bullatus Nutrition 0.000 claims abstract description 15
- 235000017181 Crataegus chrysocarpa Nutrition 0.000 claims abstract description 15
- 235000009682 Crataegus limnophila Nutrition 0.000 claims abstract description 15
- 235000004423 Crataegus monogyna Nutrition 0.000 claims abstract description 15
- 235000002313 Crataegus paludosa Nutrition 0.000 claims abstract description 15
- 235000009840 Crataegus x incaedua Nutrition 0.000 claims abstract description 15
- 239000002131 composite material Substances 0.000 claims abstract description 15
- 239000012744 reinforcing agent Substances 0.000 claims abstract description 13
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 5
- 240000000171 Crataegus monogyna Species 0.000 claims abstract 4
- 239000000203 mixture Substances 0.000 claims description 28
- 238000001035 drying Methods 0.000 claims description 20
- 239000000843 powder Substances 0.000 claims description 18
- 239000012141 concentrate Substances 0.000 claims description 12
- 238000005507 spraying Methods 0.000 claims description 12
- 229940029982 garlic powder Drugs 0.000 claims description 10
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 9
- 238000000605 extraction Methods 0.000 claims description 9
- 238000010992 reflux Methods 0.000 claims description 9
- 244000207740 Lemna minor Species 0.000 claims description 7
- 235000006439 Lemna minor Nutrition 0.000 claims description 7
- 235000001855 Portulaca oleracea Nutrition 0.000 claims description 7
- 240000004980 Rheum officinale Species 0.000 claims description 5
- 235000008081 Rheum officinale Nutrition 0.000 claims description 5
- 238000003756 stirring Methods 0.000 claims description 5
- 244000025254 Cannabis sativa Species 0.000 claims description 4
- 241000758993 Equisetidae Species 0.000 claims description 3
- 241000219061 Rheum Species 0.000 claims description 3
- 235000009411 Rheum rhabarbarum Nutrition 0.000 claims description 3
- 239000000706 filtrate Substances 0.000 claims description 3
- 238000001914 filtration Methods 0.000 claims description 3
- 238000010438 heat treatment Methods 0.000 claims description 3
- 239000007788 liquid Substances 0.000 claims description 3
- 238000010298 pulverizing process Methods 0.000 claims description 3
- 238000002156 mixing Methods 0.000 claims description 2
- 230000000694 effects Effects 0.000 abstract description 12
- 206010012455 Dermatitis exfoliative Diseases 0.000 abstract description 5
- 241001523601 Gyrodactylus Species 0.000 abstract description 4
- 206010025482 malaise Diseases 0.000 abstract description 4
- 230000002265 prevention Effects 0.000 abstract description 4
- 230000036039 immunity Effects 0.000 abstract description 3
- 235000019629 palatability Nutrition 0.000 abstract description 3
- 206010067482 No adverse event Diseases 0.000 abstract description 2
- 239000000654 additive Substances 0.000 abstract description 2
- 230000000996 additive effect Effects 0.000 abstract description 2
- 238000006243 chemical reaction Methods 0.000 abstract description 2
- 231100000957 no side effect Toxicity 0.000 abstract description 2
- 241001092040 Crataegus Species 0.000 description 11
- 241000607479 Yersinia pestis Species 0.000 description 11
- 201000010099 disease Diseases 0.000 description 11
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 11
- 241000251468 Actinopterygii Species 0.000 description 7
- 241000894006 Bacteria Species 0.000 description 6
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 6
- 238000009472 formulation Methods 0.000 description 6
- 238000000034 method Methods 0.000 description 5
- 108090000623 proteins and genes Proteins 0.000 description 4
- 102000004169 proteins and genes Human genes 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- 206010061218 Inflammation Diseases 0.000 description 3
- OYHQOLUKZRVURQ-HZJYTTRNSA-N Linoleic acid Chemical compound CCCCC\C=C/C\C=C/CCCCCCCC(O)=O OYHQOLUKZRVURQ-HZJYTTRNSA-N 0.000 description 3
- 238000009395 breeding Methods 0.000 description 3
- 230000001488 breeding effect Effects 0.000 description 3
- 150000001720 carbohydrates Chemical class 0.000 description 3
- 230000003031 feeding effect Effects 0.000 description 3
- 229910052742 iron Inorganic materials 0.000 description 3
- 229960004232 linoleic acid Drugs 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 230000001954 sterilising effect Effects 0.000 description 3
- JDLKFOPOAOFWQN-VIFPVBQESA-N Allicin Natural products C=CCS[S@](=O)CC=C JDLKFOPOAOFWQN-VIFPVBQESA-N 0.000 description 2
- XUHLIQGRKRUKPH-GCXOYZPQSA-N Alliin Natural products N[C@H](C[S@@](=O)CC=C)C(O)=O XUHLIQGRKRUKPH-GCXOYZPQSA-N 0.000 description 2
- 241000193830 Bacillus <bacterium> Species 0.000 description 2
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 2
- 241000576429 Forsythia suspensa Species 0.000 description 2
- 241000254158 Lampyridae Species 0.000 description 2
- PVNIIMVLHYAWGP-UHFFFAOYSA-N Niacin Chemical compound OC(=O)C1=CC=CN=C1 PVNIIMVLHYAWGP-UHFFFAOYSA-N 0.000 description 2
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 2
- AUNGANRZJHBGPY-SCRDCRAPSA-N Riboflavin Chemical compound OC[C@@H](O)[C@@H](O)[C@@H](O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-SCRDCRAPSA-N 0.000 description 2
- XUHLIQGRKRUKPH-UHFFFAOYSA-N S-allyl-L-cysteine sulfoxide Natural products OC(=O)C(N)CS(=O)CC=C XUHLIQGRKRUKPH-UHFFFAOYSA-N 0.000 description 2
- 241000700605 Viruses Species 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 229930013930 alkaloid Natural products 0.000 description 2
- JDLKFOPOAOFWQN-UHFFFAOYSA-N allicin Chemical compound C=CCSS(=O)CC=C JDLKFOPOAOFWQN-UHFFFAOYSA-N 0.000 description 2
- 235000010081 allicin Nutrition 0.000 description 2
- XUHLIQGRKRUKPH-DYEAUMGKSA-N alliin Chemical compound OC(=O)[C@@H](N)C[S@@](=O)CC=C XUHLIQGRKRUKPH-DYEAUMGKSA-N 0.000 description 2
- 235000015295 alliin Nutrition 0.000 description 2
- DTOSIQBPPRVQHS-PDBXOOCHSA-N alpha-linolenic acid Chemical compound CC\C=C/C\C=C/C\C=C/CCCCCCCC(O)=O DTOSIQBPPRVQHS-PDBXOOCHSA-N 0.000 description 2
- 235000020661 alpha-linolenic acid Nutrition 0.000 description 2
- 230000003260 anti-sepsis Effects 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 239000011575 calcium Substances 0.000 description 2
- 229910052791 calcium Inorganic materials 0.000 description 2
- 239000002775 capsule Substances 0.000 description 2
- 229930002875 chlorophyll Natural products 0.000 description 2
- 235000019804 chlorophyll Nutrition 0.000 description 2
- ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 235000019197 fats Nutrition 0.000 description 2
- 230000036541 health Effects 0.000 description 2
- 229910001385 heavy metal Inorganic materials 0.000 description 2
- IPCSVZSSVZVIGE-UHFFFAOYSA-N hexadecanoic acid Chemical compound CCCCCCCCCCCCCCCC(O)=O IPCSVZSSVZVIGE-UHFFFAOYSA-N 0.000 description 2
- 229960004488 linolenic acid Drugs 0.000 description 2
- KQQKGWQCNNTQJW-UHFFFAOYSA-N linolenic acid Natural products CC=CCCC=CCC=CCCCCCCCC(O)=O KQQKGWQCNNTQJW-UHFFFAOYSA-N 0.000 description 2
- 235000013372 meat Nutrition 0.000 description 2
- 150000002989 phenols Chemical class 0.000 description 2
- 239000011574 phosphorus Substances 0.000 description 2
- 229910052698 phosphorus Inorganic materials 0.000 description 2
- SCVFZCLFOSHCOH-UHFFFAOYSA-M potassium acetate Chemical compound [K+].CC([O-])=O SCVFZCLFOSHCOH-UHFFFAOYSA-M 0.000 description 2
- -1 sulfide compound Chemical class 0.000 description 2
- 235000018553 tannin Nutrition 0.000 description 2
- 239000001648 tannin Substances 0.000 description 2
- 229920001864 tannin Polymers 0.000 description 2
- 235000021122 unsaturated fatty acids Nutrition 0.000 description 2
- 235000019154 vitamin C Nutrition 0.000 description 2
- 239000011718 vitamin C Substances 0.000 description 2
- FPIPGXGPPPQFEQ-UHFFFAOYSA-N 13-cis retinol Natural products OCC=C(C)C=CC=C(C)C=CC1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-UHFFFAOYSA-N 0.000 description 1
- 125000003903 2-propenyl group Chemical group [H]C([*])([H])C([H])=C([H])[H] 0.000 description 1
- ZCYVEMRRCGMTRW-UHFFFAOYSA-N 7553-56-2 Chemical compound [I] ZCYVEMRRCGMTRW-UHFFFAOYSA-N 0.000 description 1
- WKBOTKDWSSQWDR-UHFFFAOYSA-N Bromine atom Chemical compound [Br] WKBOTKDWSSQWDR-UHFFFAOYSA-N 0.000 description 1
- WTEVQBCEXWBHNA-UHFFFAOYSA-N Citral Natural products CC(C)=CCCC(C)=CC=O WTEVQBCEXWBHNA-UHFFFAOYSA-N 0.000 description 1
- 241000186227 Corynebacterium diphtheriae Species 0.000 description 1
- AVGPOAXYRRIZMM-UHFFFAOYSA-N D-Apiose Natural products OCC(O)(CO)C(O)C=O AVGPOAXYRRIZMM-UHFFFAOYSA-N 0.000 description 1
- AUNGANRZJHBGPY-UHFFFAOYSA-N D-Lyxoflavin Natural products OCC(O)C(O)C(O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-UHFFFAOYSA-N 0.000 description 1
- ASNHGEVAWNWCRQ-LJJLCWGRSA-N D-apiofuranose Chemical compound OC[C@@]1(O)COC(O)[C@@H]1O ASNHGEVAWNWCRQ-LJJLCWGRSA-N 0.000 description 1
- ASNHGEVAWNWCRQ-UHFFFAOYSA-N D-apiofuranose Natural products OCC1(O)COC(O)C1O ASNHGEVAWNWCRQ-UHFFFAOYSA-N 0.000 description 1
- 241000238557 Decapoda Species 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- PLAPMLGJVGLZOV-UHFFFAOYSA-N Epi-orientin Natural products OC1C(O)C(O)C(CO)OC1C1=C(O)C=C(O)C2=C1OC(C=1C=C(O)C(O)=CC=1)=CC2=O PLAPMLGJVGLZOV-UHFFFAOYSA-N 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 241001596950 Larimichthys crocea Species 0.000 description 1
- 108700005090 Lethal Genes Proteins 0.000 description 1
- PTNJRKBWIYNFSY-UHFFFAOYSA-N Lirinin-O-methyl-ether Natural products COc1ccc-2c(CC3N(C)CCc4cc(OC)c(OC)c-2c34)c1 PTNJRKBWIYNFSY-UHFFFAOYSA-N 0.000 description 1
- 206010027146 Melanoderma Diseases 0.000 description 1
- 241000863420 Myxococcus Species 0.000 description 1
- 206010030113 Oedema Diseases 0.000 description 1
- RBVAFYCFAFADAG-UHFFFAOYSA-N Orientin Natural products OCC1OC(C(O)c2c(O)cc(O)c3C(=O)C=C(Oc23)c4ccc(O)c(O)c4)C(O)C1O RBVAFYCFAFADAG-UHFFFAOYSA-N 0.000 description 1
- 208000002193 Pain Diseases 0.000 description 1
- 208000004880 Polyuria Diseases 0.000 description 1
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 description 1
- 240000008801 Reseda odorata Species 0.000 description 1
- 235000002182 Reseda odorata Nutrition 0.000 description 1
- JMFSHKGXVSAJFY-UHFFFAOYSA-N Saponaretin Natural products OCC(O)C1OC(Oc2c(O)cc(O)c3C(=O)C=C(Oc23)c4ccc(O)cc4)C(O)C1O JMFSHKGXVSAJFY-UHFFFAOYSA-N 0.000 description 1
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 description 1
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 1
- 239000005864 Sulphur Substances 0.000 description 1
- 206010044565 Tremor Diseases 0.000 description 1
- LQSNPVIQIPKOGP-UHFFFAOYSA-N UNPD159785 Natural products OC1C(O)C(O)C(CO)OC1OC1=C(O)C=C(O)C2=C1OC(C=1C=C(O)C(O)=CC=1)=CC2=O LQSNPVIQIPKOGP-UHFFFAOYSA-N 0.000 description 1
- FPIPGXGPPPQFEQ-BOOMUCAASA-N Vitamin A Natural products OC/C=C(/C)\C=C\C=C(\C)/C=C/C1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-BOOMUCAASA-N 0.000 description 1
- MOZJVOCOKZLBQB-UHFFFAOYSA-N Vitexin Natural products OCC1OC(Oc2c(O)c(O)cc3C(=O)C=C(Oc23)c4ccc(O)cc4)C(O)C(O)C1O MOZJVOCOKZLBQB-UHFFFAOYSA-N 0.000 description 1
- 210000001015 abdomen Anatomy 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 150000003797 alkaloid derivatives Chemical class 0.000 description 1
- FPIPGXGPPPQFEQ-OVSJKPMPSA-N all-trans-retinol Chemical compound OC\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-OVSJKPMPSA-N 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 239000003674 animal food additive Substances 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 230000001142 anti-diarrhea Effects 0.000 description 1
- 230000000845 anti-microbial effect Effects 0.000 description 1
- 230000000840 anti-viral effect Effects 0.000 description 1
- 235000019606 astringent taste Nutrition 0.000 description 1
- 230000003385 bacteriostatic effect Effects 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000000740 bleeding effect Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 230000037396 body weight Effects 0.000 description 1
- GDTBXPJZTBHREO-UHFFFAOYSA-N bromine Substances BrBr GDTBXPJZTBHREO-UHFFFAOYSA-N 0.000 description 1
- 229910052794 bromium Inorganic materials 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 239000013043 chemical agent Substances 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 229940043350 citral Drugs 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 230000002939 deleterious effect Effects 0.000 description 1
- 238000004925 denaturation Methods 0.000 description 1
- 230000036425 denaturation Effects 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 230000000378 dietary effect Effects 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 230000035619 diuresis Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 208000001848 dysentery Diseases 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 150000002148 esters Chemical class 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- WTEVQBCEXWBHNA-JXMROGBWSA-N geranial Chemical compound CC(C)=CCC\C(C)=C\C=O WTEVQBCEXWBHNA-JXMROGBWSA-N 0.000 description 1
- 229910052732 germanium Inorganic materials 0.000 description 1
- GNPVGFCGXDBREM-UHFFFAOYSA-N germanium atom Chemical compound [Ge] GNPVGFCGXDBREM-UHFFFAOYSA-N 0.000 description 1
- 229930182478 glucoside Natural products 0.000 description 1
- 150000008131 glucosides Chemical class 0.000 description 1
- 238000005469 granulation Methods 0.000 description 1
- 230000003179 granulation Effects 0.000 description 1
- 210000000003 hoof Anatomy 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 230000003053 immunization Effects 0.000 description 1
- 230000002779 inactivation Effects 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 230000009545 invasion Effects 0.000 description 1
- PNDPGZBMCMUPRI-UHFFFAOYSA-N iodine Chemical compound II PNDPGZBMCMUPRI-UHFFFAOYSA-N 0.000 description 1
- 239000011630 iodine Substances 0.000 description 1
- 229910052740 iodine Inorganic materials 0.000 description 1
- 235000020778 linoleic acid Nutrition 0.000 description 1
- OYHQOLUKZRVURQ-IXWMQOLASA-N linoleic acid Natural products CCCCC\C=C/C\C=C\CCCCCCCC(O)=O OYHQOLUKZRVURQ-IXWMQOLASA-N 0.000 description 1
- 210000004072 lung Anatomy 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 description 1
- 239000002366 mineral element Substances 0.000 description 1
- 210000003097 mucus Anatomy 0.000 description 1
- PEFNSGRTCBGNAN-UHFFFAOYSA-N nephrocizin Natural products OC1C(O)C(O)C(CO)OC1OC1=CC(O)=C2C(=O)C=C(C=3C=C(O)C(O)=CC=3)OC2=C1 PEFNSGRTCBGNAN-UHFFFAOYSA-N 0.000 description 1
- 229960003512 nicotinic acid Drugs 0.000 description 1
- 235000001968 nicotinic acid Nutrition 0.000 description 1
- 239000011664 nicotinic acid Substances 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 235000008935 nutritious Nutrition 0.000 description 1
- 239000003921 oil Substances 0.000 description 1
- PLAPMLGJVGLZOV-VPRICQMDSA-N orientin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1C1=C(O)C=C(O)C2=C1OC(C=1C=C(O)C(O)=CC=1)=CC2=O PLAPMLGJVGLZOV-VPRICQMDSA-N 0.000 description 1
- 229940094443 oxytocics prostaglandins Drugs 0.000 description 1
- 230000003071 parasitic effect Effects 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 108090000765 processed proteins & peptides Chemical class 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 230000000644 propagated effect Effects 0.000 description 1
- 230000001902 propagating effect Effects 0.000 description 1
- 125000001436 propyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
- 150000003180 prostaglandins Chemical class 0.000 description 1
- 230000000384 rearing effect Effects 0.000 description 1
- 230000000452 restraining effect Effects 0.000 description 1
- 235000019192 riboflavin Nutrition 0.000 description 1
- 229960002477 riboflavin Drugs 0.000 description 1
- 239000002151 riboflavin Substances 0.000 description 1
- 229910052711 selenium Inorganic materials 0.000 description 1
- 239000011669 selenium Substances 0.000 description 1
- 238000007920 subcutaneous administration Methods 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 241000712461 unidentified influenza virus Species 0.000 description 1
- 150000004670 unsaturated fatty acids Chemical class 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000019155 vitamin A Nutrition 0.000 description 1
- 239000011719 vitamin A Substances 0.000 description 1
- 229940045997 vitamin a Drugs 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
- SGEWCQFRYRRZDC-VPRICQMDSA-N vitexin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1C1=C(O)C=C(O)C2=C1OC(C=1C=CC(O)=CC=1)=CC2=O SGEWCQFRYRRZDC-VPRICQMDSA-N 0.000 description 1
- PZKISQRTNNHUGF-UHFFFAOYSA-N vitexine Natural products OC1C(O)C(O)C(CO)OC1OC1=C(O)C=C(O)C2=C1OC(C=1C=CC(O)=CC=1)=CC2=O PZKISQRTNNHUGF-UHFFFAOYSA-N 0.000 description 1
- 239000000341 volatile oil Substances 0.000 description 1
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Landscapes
- Fodder In General (AREA)
- Cosmetics (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The present invention discloses loach specific complex immunostimulant and its preparation method, and this composite immune reinforcing agent is made up of the component of following weight part number: garlic 5-10 part, Radix Et Rhizoma Rhei extract 5-15 part, Fructus Forsythiae extract 1-5 part, hickory nut epicarp crude extract 1-5 part, Folium Lauri nobilis 4-6 part, Cortex Meliae 2-3 part, Folium Populi Pseudo-simonii 1-5 part, Herba Spirodelae extract 5-10 part, hawthorn 10-15 part, salt 10-20 part. The present invention as additive and conventional feed with the use of, loach immunity of organisms can be strengthened, it is to increase resistibility; Having no side effect and untoward reaction, the palatability for feed has no adverse effects, garlic, Radix Et Rhizoma Rhei extract, Fructus Forsythiae extract, hickory nut epicarp runic thing and salt with the use of, can annual prevention of water mildew, reduce sickness rate; Wheel worm and Gyrodactylus can be played the effect killed by Folium Lauri nobilis, Cortex Meliae, Folium Populi Pseudo-simonii; Prevention erythroderma is had good effect by Herba Spirodelae extract.
Description
Technical field
The invention belongs to field of feed additive technology, it is specifically related to a kind of loach specific complex immunostimulant and its preparation method.
Background technology
Loach, another name fish loach, body is elongated, and front section slightly cylindrical. Rear portion is flat-sided, belly circle, little. Young, the next, the shape of a hoof. Eye is little, without stinging now. Palpus 5. Squama is extremely tiny, circular, is embedded in subcutaneous. Body back and both sides grey black, entirety has many little black patchess, and head and each fin also have many black splotches, and the spot arrangement on dorsal fin and tail fin film is embarked on journey, and there is an obvious blackspot in caudal peduncle base portion. Loach is distributed widely in the ground such as China of Asia bank, Japan, Korea, Russia and India.
Mineral element and a large amount of VITAMIN such as loach rich in proteins, fat, carbohydrate and calcium, phosphorus, iron, wherein the content of VITMAIN B1 exceeds 3��4 times than fish, yellow croaker, shrimp, and the content of vitamin A, vitamins C and iron is also high than other fish. the light delicacy of loach meat, the delicious U.S. of meat, nutritious, and there is good dietary function. the market demand of loach increases in recent years, and supply falls short of demand. the export situation of loach is fine, and Japan, Korea S's demand are very big, and order increases year by year. therefore, natural fish for loach and far can not meet the need of market, loach is propagated artificially and starts to rise, often easily there is various disease and pest in breeding process in loach, especially annual three, April is easy to fish molds occur, loach common has wheel worm in the seed rearing stage, tongue cup worm, Gyrodactylus is parasitic and causes death, bubble illness and erythroderma are also that loach is in breeding process, it is easy to introduced disease, in prior art, for loach common disease and pest, normally chemical agent is adopted to kill again after finding disease and pest, instead of adopt the measure prevented in advance, this kind of chemical reagent is killed, slight disease and pest can be played certain effect, but for the disease and pest that the oncoming force is more violent, under normal circumstances, effect is not fine, the death of large quantities of loach can be caused, certain financial loss is brought to raiser, this kind of mode carries out killing of disease and pest, just play interim killing, can not fundamentally cure, the generation of disease and pest, cause the immunity degradation of loach, mortality ratio increases, seriously have impact on the economic benefit of the speed of growth of loach and cultivation, how to prevent before disease and pest occurs, improve the invasion of fish body opposing disease and pest, reduce sickness rate, become crucial. in the breeding process of loach, throwing something and feeding of feed is a step of most critical, and the raising of the resistibility of loach own, also closely related with throwing something and feeding of feed, therefore, feed adds composite immune reinforcing agent, to regulate body health, enhancing body immunizing power, restraining and sterilizing bacteria pest-resistant by feed of throwing something and feeding, propagating artificially of loach is extremely important.
Summary of the invention
For problems of the prior art, the object of the invention is at a kind of loach specific complex immunostimulant of offer and its preparation method.
The present invention is realized by the following technical programs:
Described loach specific complex immunostimulant, it is characterised in that this composite immune reinforcing agent is made up of the component of following weight part number: garlic 5-10 part, Radix Et Rhizoma Rhei extract 5-15 part, Fructus Forsythiae extract 1-5 part, hickory nut epicarp crude extract 1-5 part, Folium Lauri nobilis 4-6 part, Cortex Meliae 2-3 part, Folium Populi Pseudo-simonii 1-5 part, Herba Spirodelae extract 5-10 part, hawthorn 10-15 part, salt 10-20 part.
Described loach specific complex immunostimulant, it is characterised in that be made up of the component of following weight part number: 7 parts, garlic, Radix Et Rhizoma Rhei extract 10 parts, Fructus Forsythiae extract 3 parts, hickory nut epicarp crude extract 2 parts, Folium Lauri nobilis 5 parts, Cortex Meliae 3 parts, Folium Populi Pseudo-simonii 3 parts, Herba Spirodelae extract 8 parts, hawthorn 15 parts, salt 20 parts.
Described loach specific complex immunostimulant, it is characterised in that this composite immune reinforcing agent adds in loach mixed feed, the use ratio of composite immune reinforcing agent and loach mixed feed is 1:50-80.
Described loach specific complex immunostimulant, it is characterised in that described composite immune reinforcing agent adds in loach mixed feed, the use ratio of composite immune reinforcing agent and loach mixed feed is 1:65.
The preparation method of described loach specific complex immunostimulant, it is characterised in that follow these steps to carry out:
1) take the big Folium Lauri nobilis of formula ratio, Cortex Meliae, Folium Populi Pseudo-simonii, hawthorn respectively, clean clean after dry in drying baker, after drying, each composition is pulverized respectively, wears into fine powder, crosses 40 orders sieves, obtains each composition dry powder, for subsequent use;
2) the fresh garlic head after the peeling of formula ratio is got, with pulverizer, Bulbus Allii head is processed into mashed garlic, mashed garlic is put into baking oven, dry with slow fire, temperature controls at 65 DEG C, until mashed garlic becomes block, in drying course, stirred mashed garlic every 1 hour once, mashed garlic is heated evenly, mashed garlic after drying is pulverized in pulverizer, crosses 60 order sieves, obtain garlic powder;
3) by step 2) after the obtained each composition dry powder blend of obtained garlic powder, step 1), add the Radix Et Rhizoma Rhei extract of formula ratio, Fructus Forsythiae extract, hickory nut epicarp crude extract, Herba Spirodelae extract, salt mixing, stir evenly.
The preparation method of described loach specific complex immunostimulant, it is characterized in that the preparation method of described Radix Et Rhizoma Rhei extract is: rheum officinale is pulverized, cross 40 order sieves, by 90% ethanolic soln refluxing extraction, extract 1-2 hour every time, repeat to extract 2-3 time, the weightmeasurement ratio of rhubarb horsetails powder and ethanol is 1:15-20g/ml, extracting solution concentrates to medicinal extract, and medicinal extract spraying dry under the temperature condition of 165 DEG C, obtains Radix Et Rhizoma Rhei extract.
The preparation method of described loach specific complex immunostimulant, it is characterized in that the preparation method of described Fructus Forsythiae extract is: Folium Forsythiae is pulverized, cross 40 order sieves, the water of times weight that adds 15 refluxing extraction 2-3 hour, extracting liquid filtering, filtrate concentrates to medicinal extract, and medicinal extract spraying dry under the temperature condition of 165 DEG C, obtains Fructus Forsythiae extract.
The preparation method of described loach specific complex immunostimulant, it is characterized in that the preparation method of described hickory nut epicarp crude extract is: hickory nut epicarp is dried, pulverized, cross 40 order sieves, the alcohol steep adding 75% 48 hours, vat liquor concentrates to medicinal extract, medicinal extract spraying dry under the temperature condition of 170 DEG C, obtains hickory nut epicarp crude extract.
The preparation method of described loach specific complex immunostimulant, it is characterized in that the preparation method of described Herba Spirodelae extract is: duckweed is grass oven dry pulverizing entirely, adding 20 times of concentration by weight is the ethanolic soln of 90%, heating and refluxing extraction 1-3 time, each 1-1.5 hour, extracting solution merged, and concentrates to medicinal extract, medicinal extract spraying dry under the temperature condition of 170 DEG C, obtains Herba Spirodelae extract.
Garlic nutrient enriches: every 100 grams of moisture contents 69.8 grams, 4.4 grams, protein, 0.2 gram, fat, 23.6 grams, carbohydrate, calcium 5 milligrams, 44 milligrams, phosphorus, iron 0.4 milligram, vitamins C 3 milligrams. In addition, also containing VitB1, riboflavin, nicotinic acid, allicin, citral and the trace element such as selenium and germanium. Containing volatile oil about 0.2%, in oil, main component is allicin, and the alliin being contained in garlic is produced by the effect hydrolysis of big allinase. Still containing the sulfide compound etc. of multiple allyl group, propyl group and methyl composition. Garlic has powerful sterilizing ability, can eliminate the germ in intrusive body. It also helps the absorption of VITMAIN B1, promotes that the metabolism of carbohydrate is with generate energy, and alleviating fatigue. Garlic integrates more than 100 kind of medicine and health care composition, wherein containing sulphur volatile matter 43 kinds, and sulfuration-sulfinic acid (such as garlicin) ester class 13 kinds, 9 kinds, amino acid, peptide class 8 kinds, glucoside class 12 kinds, enzyme class 11 kinds. In addition, alliin is the unique composition of garlic, just becomes garlicin when it enters blood, even if this kind of garlicin dilution 100,000 times still can kill Corynebacterium diphtheriae, dysentery bacterium, influenza virus etc. instantaneously.
Rheum officinale, the capsule of weeping forsythia are medicinal plant, have effect of antisepsis and anti-inflammation, and both are composite, its antibiotic and sterilizing ability strengthens, rheum officinale, the capsule of weeping forsythia, hickory nut epicarp crude extract and garlic and salt are used in combination, can effectively prevent the generation of fish molds, also can improve the immunological competence of human body. The generation of loach wheel worm and Gyrodactylus can be effectively avoided together with Folium Lauri nobilis is composite with Cortex Meliae and Folium Populi Pseudo-simonii.
Duckweed nature and flavor are pungent, tremble with fear, return lung (gill), bladder warp. The effects such as tool is clearing heat and detoxicating, promoting eruption, inducing diuresis to remove edema. Grass is main containing orientin, Vitexin, apiose, Reseda odorata flavine-7-glycoside entirely for duckweed. Still containing Potassium ethanoate, Repone K, iodine, bromine, chlorophyll, tannin and lipoid cpd (linolenic acid, palmitinic acid, linolic acid) etc. Wherein, tannin is the phenolic compound of a class complexity, has astringent taste and convergency, can be combined with protein, mucus, alkaloid salt, heavy metallic salt and generate precipitation. Phenols has anti-microbial effect, and the deleterious effect of bacterium may mainly be made protein denaturation by it, has again the effect of tensio-active agent simultaneously, destroys membrane passage, makes cell content excessive. It is lethal gene when concentration height, otherwise then plays bacteriostatic action. Therefore it is conventional as astringing to arrest bleeding, antidiarrheal, antisepsis and anti-inflammation. Can be used as the toxinicide of alkaloid and heavy metal (be combined with heavy metal and generate coordination compound, and excrete) in addition. The solubility salt of chlorophyll has certain antibacterial and anti-inflammation functions and promotes the effect of granulation growth. Iodine is strong sterilant, can kill bacterium, fungi, and make inactivation of virus. Potassium ethanoate is strong base-weak acid salt, in alkalescence, can constantly neutralize the acid that bacterium produces, and highly basic is stronger than gram positive bacterium effect to gram negative bacterium and virus. The pathogenic agent of erythroderma is firefly light pole hair bacillus, is Gram-negative mattress. Therefore, duckweed not only can anti-fish evil myxococcus and the firefly light pole hair bacillus of erythroderma, and can be antiviral. In addition, duckweed is also containing the unsaturated fatty acids such as linolenic acid, palmitinic acid, linolic acid, and its Linoleic acid is the most important unsaturated fatty acidss of fish, can synthesis of prostaglandins.
Compared with existing preparation, the present invention has the following advantages:
1) as additive and loach conventional feed with the use of, garlic, Radix Et Rhizoma Rhei extract, Fructus Forsythiae extract, hickory nut epicarp runic thing and salt with the use of, can annual prevention of water mildew, reduce sickness rate;
2) Folium Lauri nobilis, Cortex Meliae, Folium Populi Pseudo-simonii and feed formulation use, and wheel worm and Gyrodactylus can play the effect killed;
3) interpolation of Herba Spirodelae extract, has good effect to prevention erythroderma;
4) cooperation of each composition, can strengthen loach immunity of organisms, it is to increase to the resistibility of disease and pest;
5) having no side effect and untoward reaction, the palatability for feed has no adverse effects, the interpolation of hawthorn, is convenient to improve the palatability of feed.
Embodiment
By following specific embodiment, the invention will be further described.
Embodiment 1
Loach specific complex immunostimulant, consists of: 7 parts, garlic, Radix Et Rhizoma Rhei extract 10 parts, Fructus Forsythiae extract 3 parts, hickory nut epicarp crude extract 2 parts, Folium Lauri nobilis 5 parts, Cortex Meliae 3 parts, Folium Populi Pseudo-simonii 3 parts, Herba Spirodelae extract 8 parts, hawthorn 15 parts, salt 20 parts. Its preparation method is: take the big Folium Lauri nobilis of formula ratio, Cortex Meliae, Folium Populi Pseudo-simonii, hawthorn respectively, clean clean after dry in drying baker, after drying, each composition is pulverized respectively, wears into fine powder, crosses 40 orders sieves, obtains each composition dry powder; Get the fresh garlic head after the peeling of formula ratio, with pulverizer, Bulbus Allii head is processed into mashed garlic, mashed garlic is put into baking oven, dry with slow fire, temperature controls at 65 DEG C, until mashed garlic becomes block, in drying course, stirred mashed garlic every 1 hour once, mashed garlic is heated evenly, mashed garlic after drying is pulverized in pulverizer, crosses 60 order sieves, obtain garlic powder; The Radix Et Rhizoma Rhei extract of each composition dry powder of above-mentioned preparation and garlic powder and formula ratio, Fructus Forsythiae extract, hickory nut epicarp crude extract, Herba Spirodelae extract, salt are mixed, stirs evenly i.e. obtained loach specific complex immunostimulant. Getting the loach specific complex immunostimulant 1 part prepared, the loach conventional feed with 65 parts mixes, and is thrown something and fed by loach.
Wherein: the preparation method of Radix Et Rhizoma Rhei extract is: rheum officinale is pulverized, cross 40 order sieves, by 90% ethanolic soln refluxing extraction, extract 1-2 hour every time, repeating to extract 2-3 time, the weightmeasurement ratio of rhubarb horsetails powder and ethanol is 1:15-20g/ml, and extracting solution concentrates to medicinal extract, medicinal extract spraying dry under the temperature condition of 165 DEG C, obtains Radix Et Rhizoma Rhei extract.
The preparation method of Fructus Forsythiae extract is: Folium Forsythiae is pulverized, and crosses 40 orders sieves, and the water of times weight that adds 15 refluxing extraction 2-3 hour, extracting liquid filtering, filtrate concentrates to medicinal extract, and medicinal extract spraying dry under the temperature condition of 165 DEG C, obtains Fructus Forsythiae extract.
The preparation method of hickory nut epicarp crude extract is: hickory nut epicarp is dried, pulverized, and crosses 40 order sieves, and the alcohol steep adding 75% 48 hours, vat liquor concentrates to medicinal extract, and medicinal extract spraying dry under the temperature condition of 170 DEG C, obtains hickory nut epicarp crude extract.
The preparation method of Herba Spirodelae extract is: duckweed is grass oven dry pulverizing entirely, and adding 20 times of concentration by weight is the ethanolic soln of 90%, heating and refluxing extraction 1-3 time, each 1-1.5 hour, extracting solution merged, and concentrates to medicinal extract, medicinal extract spraying dry under the temperature condition of 170 DEG C, obtains Herba Spirodelae extract.
Embodiment 2
Loach specific complex immunostimulant, consists of: 5 parts, garlic, Radix Et Rhizoma Rhei extract 15 parts, Fructus Forsythiae extract 1 part, hickory nut epicarp crude extract 5 parts, Folium Lauri nobilis 4 parts, Cortex Meliae 3 parts, Folium Populi Pseudo-simonii 1 part, Herba Spirodelae extract 10 parts, hawthorn 10 parts, salt 20 parts. Its preparation method is: take the big Folium Lauri nobilis of formula ratio, Cortex Meliae, Folium Populi Pseudo-simonii, hawthorn respectively, clean clean after dry in drying baker, after drying, each composition is pulverized respectively, wears into fine powder, crosses 40 orders sieves, obtains each composition dry powder; Get the fresh garlic head after the peeling of formula ratio, with pulverizer, Bulbus Allii head is processed into mashed garlic, mashed garlic is put into baking oven, dry with slow fire, temperature controls at 65 DEG C, until mashed garlic becomes block, in drying course, stirred mashed garlic every 1 hour once, mashed garlic is heated evenly, mashed garlic after drying is pulverized in pulverizer, crosses 60 order sieves, obtain garlic powder; The Radix Et Rhizoma Rhei extract of each composition dry powder of above-mentioned preparation and garlic powder and formula ratio, Fructus Forsythiae extract, hickory nut epicarp crude extract, Herba Spirodelae extract, salt are mixed, stirs evenly i.e. obtained loach specific complex immunostimulant. Getting the loach specific complex immunostimulant 1 part prepared, the loach conventional feed with 50 parts mixes, and is thrown something and fed by loach.
The extracting method of Radix Et Rhizoma Rhei extract, Fructus Forsythiae extract, hickory nut epicarp crude extract, Herba Spirodelae extract is with embodiment 1.
Embodiment 3
Loach specific complex immunostimulant, consists of: 10 parts, garlic, Radix Et Rhizoma Rhei extract 5 parts, Fructus Forsythiae extract 5 parts, hickory nut epicarp crude extract 1 part, Folium Lauri nobilis 6 parts, Cortex Meliae 2 parts, Folium Populi Pseudo-simonii 5 parts, Herba Spirodelae extract 5 parts, hawthorn 5 parts, salt 10 parts. Its preparation method is: take the big Folium Lauri nobilis of formula ratio, Cortex Meliae, Folium Populi Pseudo-simonii, hawthorn respectively, clean clean after dry in drying baker, after drying, each composition is pulverized respectively, wears into fine powder, crosses 40 orders sieves, obtains each composition dry powder; Get the fresh garlic head after the peeling of formula ratio, with pulverizer, Bulbus Allii head is processed into mashed garlic, mashed garlic is put into baking oven, dry with slow fire, temperature controls at 65 DEG C, until mashed garlic becomes block, in drying course, stirred mashed garlic every 1 hour once, mashed garlic is heated evenly, mashed garlic after drying is pulverized in pulverizer, crosses 60 order sieves, obtain garlic powder; The Radix Et Rhizoma Rhei extract of each composition dry powder of above-mentioned preparation and garlic powder and formula ratio, Fructus Forsythiae extract, hickory nut epicarp crude extract, Herba Spirodelae extract, salt are mixed, stirs evenly i.e. obtained loach specific complex immunostimulant. Getting the loach specific complex immunostimulant 1 part prepared, the loach conventional feed with 80 parts mixes, and is thrown something and fed by loach.
The extracting method of Radix Et Rhizoma Rhei extract, Fructus Forsythiae extract, hickory nut epicarp crude extract, Herba Spirodelae extract is with embodiment 1.
Control group 1
Loach specific complex immunostimulant, consists of: 4 parts, garlic, Radix Et Rhizoma Rhei extract 17 parts, Fructus Forsythiae extract 6 parts, hickory nut epicarp crude extract 0.5 part, Folium Lauri nobilis 2 parts, Cortex Meliae 5 parts, Folium Populi Pseudo-simonii 0.5 part, Herba Spirodelae extract 15 parts, hawthorn 8 parts, salt 5 parts. Preparation method is with embodiment 1. Getting loach specific complex immunostimulant 1 part, the loach conventional feed with 65 parts mixes, and is thrown something and fed by loach.
Control group 2
Adopt and throw something and feed with the conventional feed that embodiment 1 is identical, do not add composite immune reinforcing agent.
Test example
Pond is separated into five regions of the same area, it is numbered 1,2,3,4,5, in the beginning of June, throws in loach seedling 200 tail that body length is about 3cm respectively to each marker space, wherein, 1 throws something and feeds according to the formulation content of embodiment 1, and 2 throw something and feed according to the formulation content of embodiment 2, and 3 throw something and feed according to the formulation content of embodiment 3,4 throw something and feed according to the formulation content of control group, 5 throw something and feed according to control group 2 formulation content, feed 6 months, and feeding effect is in table 1.
Table 1 feeding effect
Experimental result shows: when ambient conditions is identical, and loach specific complex immunostimulant of the present invention can significantly improve rate of growth and the body weight of loach, reduces the sickness rate of loach, it is to increase the survival rate of loach, reduces feed coefficient. As seen from Table 1; the loach of No. 1 isolated area that employing embodiment 1 content formula is thrown something and fed; every index is all better than other each group; the loach of No. 4 isolated areas that employing control group 1 content formula is thrown something and fed, owing to the content of each composition of control group 1 is not all in the protection domain of present patent application, its every index fed is all lower than the feeding effect of embodiment 1-3; as can be seen from the table; its surviving rate is on the low side, is because the addition of Herba Spirodelae extract is on the high side, causes the dead quantity of loach fry to increase.
Claims (9)
1. loach specific complex immunostimulant, it is characterised in that this composite immune reinforcing agent is made up of the component of following weight part number: garlic 5-10 part, Radix Et Rhizoma Rhei extract 5-15 part, Fructus Forsythiae extract 1-5 part, hickory nut epicarp crude extract 1-5 part, Folium Lauri nobilis 4-6 part, Cortex Meliae 2-3 part, Folium Populi Pseudo-simonii 1-5 part, Herba Spirodelae extract 5-10 part, hawthorn 10-15 part, salt 10-20 part.
2. loach specific complex immunostimulant according to claim 1, it is characterised in that be made up of the component of following weight part number: 7 parts, garlic, Radix Et Rhizoma Rhei extract 10 parts, Fructus Forsythiae extract 3 parts, hickory nut epicarp crude extract 2 parts, Folium Lauri nobilis 5 parts, Cortex Meliae 3 parts, Folium Populi Pseudo-simonii 3 parts, Herba Spirodelae extract 8 parts, hawthorn 15 parts, salt 20 parts.
3. loach specific complex immunostimulant according to claim 1 and 2, it is characterised in that this composite immune reinforcing agent adds in loach mixed feed, and the use ratio of composite immune reinforcing agent and loach mixed feed is 1:50-80.
4. loach specific complex immunostimulant according to claim 3, it is characterised in that described composite immune reinforcing agent adds in loach mixed feed, and the use ratio of composite immune reinforcing agent and loach mixed feed is 1:65.
5. the preparation method of loach specific complex immunostimulant, it is characterised in that follow these steps to carry out:
1) take the big Folium Lauri nobilis of formula ratio, Cortex Meliae, Folium Populi Pseudo-simonii, hawthorn respectively, clean clean after dry in drying baker, after drying, each composition is pulverized respectively, wears into fine powder, crosses 40 orders sieves, obtains each composition dry powder, for subsequent use;
2) the fresh garlic head after the peeling of formula ratio is got, with pulverizer, Bulbus Allii head is processed into mashed garlic, mashed garlic is put into baking oven, dry with slow fire, temperature controls at 65 DEG C, until mashed garlic becomes block, in drying course, stirred mashed garlic every 1 hour once, mashed garlic is heated evenly, mashed garlic after drying is pulverized in pulverizer, crosses 60 order sieves, obtain garlic powder;
3) by step 2) after the obtained each composition dry powder blend of obtained garlic powder, step 1), add the Radix Et Rhizoma Rhei extract of formula ratio, Fructus Forsythiae extract, hickory nut epicarp crude extract, Herba Spirodelae extract, salt mixing, stir evenly.
6. the preparation method of loach specific complex immunostimulant according to claim 5, it is characterized in that the preparation method of described Radix Et Rhizoma Rhei extract is: rheum officinale is pulverized, cross 40 order sieves, by 90% ethanolic soln refluxing extraction, extract 1-2 hour every time, repeat to extract 2-3 time, the weightmeasurement ratio of rhubarb horsetails powder and ethanol is 1:15-20g/ml, extracting solution concentrates to medicinal extract, and medicinal extract spraying dry under the temperature condition of 165 DEG C, obtains Radix Et Rhizoma Rhei extract.
7. the preparation method of loach specific complex immunostimulant according to claim 5, it is characterized in that the preparation method of described Fructus Forsythiae extract is: Folium Forsythiae is pulverized, cross 40 order sieves, the water of times weight that adds 15 refluxing extraction 2-3 hour, extracting liquid filtering, filtrate concentrates to medicinal extract, and medicinal extract spraying dry under the temperature condition of 165 DEG C, obtains Fructus Forsythiae extract.
8. the preparation method of loach specific complex immunostimulant according to claim 5, it is characterized in that the preparation method of described hickory nut epicarp crude extract is: hickory nut epicarp is dried, pulverized, cross 40 order sieves, the alcohol steep adding 75% 48 hours, vat liquor concentrates to medicinal extract, medicinal extract spraying dry under the temperature condition of 170 DEG C, obtains hickory nut epicarp crude extract.
9. the preparation method of loach specific complex immunostimulant according to claim 5, it is characterized in that the preparation method of described Herba Spirodelae extract is: duckweed is grass oven dry pulverizing entirely, adding 20 times of concentration by weight is the ethanolic soln of 90%, heating and refluxing extraction 1-3 time, each 1-1.5 hour, extracting solution merged, and concentrates to medicinal extract, medicinal extract spraying dry under the temperature condition of 170 DEG C, obtains Herba Spirodelae extract.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201410517625.5A CN104222680B (en) | 2014-09-30 | 2014-09-30 | Loach specific complex immunostimulant and its preparation method |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201410517625.5A CN104222680B (en) | 2014-09-30 | 2014-09-30 | Loach specific complex immunostimulant and its preparation method |
Publications (2)
Publication Number | Publication Date |
---|---|
CN104222680A CN104222680A (en) | 2014-12-24 |
CN104222680B true CN104222680B (en) | 2016-06-08 |
Family
ID=52212288
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201410517625.5A Expired - Fee Related CN104222680B (en) | 2014-09-30 | 2014-09-30 | Loach specific complex immunostimulant and its preparation method |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN104222680B (en) |
Families Citing this family (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105815601A (en) * | 2016-03-21 | 2016-08-03 | 沈阳工学院 | Immunopotentiator special for medaka and preparation method and application of immunopotentiator |
CN106306385A (en) * | 2016-08-19 | 2017-01-11 | 怀远县渔业科技发展有限责任公司 | Attracting immunity-improving feed additive for oriental weatherfish and preparation method thereof |
CN106260780A (en) * | 2016-08-19 | 2017-01-04 | 怀远县渔业科技发展有限责任公司 | A kind of feed additive for treating Misgurni anguillicaudati edema disease and preparation method thereof |
CN106858061A (en) * | 2017-03-24 | 2017-06-20 | 河南科高中标检测技术有限公司 | A kind of application for the composition of natural products of additive for farm animal feed and its in feed addictive |
CN108617887A (en) * | 2018-05-11 | 2018-10-09 | 宿松县龙源水产养殖专业合作社 | A kind of loach summer heat-clearing appetizer |
CN108888699A (en) * | 2018-07-25 | 2018-11-27 | 贵州贵丰农业发展有限公司 | A method of prevention and treatment soft-shelled turtle saprolegniasis |
Family Cites Families (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH08116887A (en) * | 1994-10-27 | 1996-05-14 | Solar Japan Kk | Method for culturing loach fry |
KR20000053916A (en) * | 2000-05-08 | 2000-09-05 | 전효천 | How to prepare Chueotang for removing fishy taste |
CN1270622C (en) * | 2005-01-11 | 2006-08-23 | 冯绍宽 | Plant additive premixed forage for aquatic culture and bait therefrom |
CN102125190B (en) * | 2011-03-02 | 2012-09-05 | 江苏华威农牧发展有限公司 | Loach feed bred in high-density and scaled mode |
CN102293335A (en) * | 2011-07-01 | 2011-12-28 | 广西粤海饲料有限公司 | Universal composite Chinese herbal immunopotentiator for fish cultured in freshwater |
CN103844145A (en) * | 2014-03-12 | 2014-06-11 | 江苏天成科技集团有限公司 | Method for producing novel loach feed |
CN104041440A (en) * | 2014-05-29 | 2014-09-17 | 郎溪县兴贵水产品养殖家庭农场 | Raising method with function of facilitating growth of misgurnus anguillicaudatus |
-
2014
- 2014-09-30 CN CN201410517625.5A patent/CN104222680B/en not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
---|---|
CN104222680A (en) | 2014-12-24 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN104222680B (en) | Loach specific complex immunostimulant and its preparation method | |
CN103141710B (en) | Compound feed for weaned pigs and preparation method of feed | |
CN102845638B (en) | Duck feed capable of preventing and treating avian influenza and Chinese herbal additive used therein | |
CN103931686A (en) | Plant pesticide as well as preparation method and application thereof | |
KR102213743B1 (en) | A process and the composition for increasing immunity-boosting and the growth of animals comprising herbs | |
CN104095153A (en) | Special-purpose immunopotentiating agent for dungat groupers | |
CN102919607B (en) | Chinese herbal medicine compound feed additive with anti-diarrhea function and improving pork quality | |
CN104206914B (en) | A kind of manufacture method of Rrinaceus earopaeus formula forage | |
KR101737271B1 (en) | Natural extract containing a large amount of Omega-3 and Manufacturing method thereof | |
Sorwar et al. | Effect of kalo jeera seeds and papaya leaf supplementation on the performance of broiler. | |
CN105815611A (en) | Chinese herbal attractant for aquatic animals | |
KR20020032807A (en) | Meat of Duck Fed on Garlic and/or Oriental Medicines and Method of Feeding the Duck | |
CN106578484A (en) | Chinese herbal medicine feed supplement for sows in suckling period | |
KR20120095505A (en) | Foot-and-mouth disease prevention useful feed and method of preparing thereof | |
RU2641907C1 (en) | Bioactive fodder additive for agricultural animals and poultry | |
CN103918935A (en) | Pet food additive | |
CN107296173A (en) | Improve aquatic livestock immunity feed and preparation method thereof | |
Bottoni et al. | Alpine ethnobotanical knowledge in Sondalo (SO, Lombardy, Italy) | |
CN106359203A (en) | Method for improving quality of fish | |
KR100466946B1 (en) | Feed for Duck and Method of Feeding Duck with the Feed | |
CN105341534A (en) | Feed additive for preventing white-spot disease of parrot fishes and preparation method thereof | |
Amad et al. | Moringa (M. oleifera) leaf meal in diets for broilers and laying hens: a review | |
Popović et al. | Medicinal properties of buckwheat products and honey in compliance with food safety regulatory requirements | |
KR102582313B1 (en) | Composition for improving joint health by reducing body fat in companion dogs | |
CN106614178A (en) | Composite preparation for preventing and treating water retention disease of river crab and application thereof |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20160608 Termination date: 20180930 |
|
CF01 | Termination of patent right due to non-payment of annual fee |