CN103860624B - Method for removing fructus trichosanthis pulp by assist of biological enzyme - Google Patents
Method for removing fructus trichosanthis pulp by assist of biological enzyme Download PDFInfo
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- CN103860624B CN103860624B CN201410116545.9A CN201410116545A CN103860624B CN 103860624 B CN103860624 B CN 103860624B CN 201410116545 A CN201410116545 A CN 201410116545A CN 103860624 B CN103860624 B CN 103860624B
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Abstract
The invention relates to a method for removing fructus trichosanthis pulp by assist of a biological enzyme, belongs to the field of medicament producing and processing, and aims at solving the technical problem of providing a method for removing fructus trichosanthis pulp by assist of a biological enzyme, and use of amylase and cellulose for removing the fructus trichosanthis pulp. The method for removing the fructus trichosanthis pulp by assist of the biological enzyme comprises the following steps: putting fructus trichosanthis seeds with pulp into a biological enzyme solution; taking the fructus trichosanthis seeds to wash and dry after separating the fructus trichosanthis seeds from the pulp, so as to obtain the fructus trichosanthis seeds. Furthermore, the invention also discloses use of amylase and cellulose for removing pulp. The method for removing the fructus trichosanthis pulp by assist of the biological enzyme has the advantages of being simple in process, convenient to operate, low in cost, high in removal efficiency and the like.
Description
Technical field
The present invention relates to the method that enzyme auxiliary sloughs Snakegourd Fruit seed, belongs to drug manufacture manufacture field.
Background technology
Fructus Trichosanthis are the perennial dioecism liana of Cucurbitaceae, and the large traditional Chinese medicinal materials assortment of dietotherapeutic, applicating history are swung
Long, medical value is high, at home and abroad has long enjoyed a good reputation.Fructus Trichosanthis not only have very high medical value, and with higher nutrition
And health value, its peel, kernel, root make medicinal, often to eat Snakegourd Fruit seed to protection breathing and cardiovascular system, suppress cancer thin
Born of the same parents, treatment diabetes, treatment " three high " and raising body immune function, adjustment endocrine have good effect.
As people go deep into Fructus Trichosanthis understanding, its value increasingly obtains everybody accreditation, and Snakegourd Fruit seed is edible, medicinal
Upper demand increases, and on the ground such as Sichuan, Anhui, Zhejiang, Shandong, cultivated area is also increasingly wider, but by Snakegourd Fruit seed from snake gourd fruit pulp
In the technique that is stripped out it is but very loaded down with trivial details.Seed is tightly bundled together by snake gourd fruit pulp, and after peelling off Pericarpium Trichosanthiss, snake gourd fruit pulp is tightly
Be wrapped in Snakegourd Fruit seed, many fleshes of seed are hard, and seed is embedded very deep, therefore want quickly successfully to peel off Snakegourd Fruit seed and do not allow very much
Easily.In production practices medicinal herb growers generally by the Snakegourd Fruit seed with snake gourd fruit pulp be put in woven bag sealing or with plastic sheeting by its
Cover, then water water so as to which snake gourd fruit pulp is corrupt, then after 12～14 days, by snake gourd fruit pulp eluting, obtains Snakegourd Fruit seed, generally
Adopt and hand-launder or in container help snake gourd fruit pulp to depart from waddy stirring；In cleaning process, snake gourd fruit pulp gives out strong thorn
Ozena taste, turn one's stomach vomiting, and many people are difficult to stand, and therefore Fructus Trichosanthis also have a popular name " dung melon seed ".But even
Through above-mentioned process, snake gourd fruit pulp is still difficult eluting；Cleaning 6～10 times is generally also needed to, Snakegourd Fruit seed could be eluted out, such as
Fruit hand-launders, and the injury of opponent is very big.Therefore the method for traditional eluting Snakegourd Fruit seed, consumes substantial amounts of manpower and materials, to big
The melon grower of amount plantation brings greatly puzzlement.Therefore, find a kind of simple, convenient, with low cost side for sloughing snake gourd fruit pulp
Method, is problem demanding prompt solution.
The content of the invention
The technical problem to be solved in the present invention is to provide a kind of method and starch that enzyme auxiliary sloughs snake gourd fruit pulp
Enzyme, cellulase are used for the purposes for sloughing snake gourd fruit pulp.
The method that enzyme auxiliary of the present invention sloughs snake gourd fruit pulp, it is characterised in that comprise the steps:By the Fructus Trichosanthis with flesh
Seed is put in biological enzymatic solution, after Snakegourd Fruit seed is separated with flesh, takes Snakegourd Fruit seed, washing, is dried, is obtained final product Snakegourd Fruit seed.
Wherein, the enzyme in biological enzymatic solution can hydrolyze the organic principle in snake gourd fruit pulp, so as to cause snake gourd fruit pulp and melon
Beach wormwood seed is separated.
Further, effect is sloughed in order to take into account cost and snake gourd fruit pulp, described enzyme auxiliary sloughs the side of snake gourd fruit pulp
Method, comprises the steps：
In a, the biological enzymatic solution that will be put into flesh Snakegourd Fruit seed, wherein, the solid-liquid ratio with flesh Snakegourd Fruit seed with biological enzymatic solution
For 3～8:3～8g/ml, enzyme solution temperature are 25～35 DEG C；
B, 25～35 DEG C of holding enzyme solution temperature, stand 60～120h；
C, Snakegourd Fruit seed is taken, washed, be dried, obtain final product.
Further, in order to reduce production cost, while ensure that snake gourd fruit pulp can be efficiently separated, the biological enzymatic solution
Concentration is preferably 0.05～0.75wt%, most preferably more preferably 0.15～0.65wt%, 0.25wt%.
Further, for improve production efficiency, enzyme of the present invention is adopted in aiding in the method for slough snake gourd fruit pulp
Enzyme be preferably amylase, at least one in cellulase；Further, the amylase is preferably α-amylase；
At least one in the preferred endoglucanase of the cellulase, exoglucanase, beta glucan glycosides enzyme.
Further, enzymatic solution consumption can excessively cause the waste of raw material, and consumption is crossed
Really, in order to accelerate snake gourd fruit pulp decomposition rate, while make the eluting of Snakegourd Fruit seed cleaner, preferably, band flesh melon in a steps
Beach wormwood seed is 4～7 with the solid-liquid ratio of biological enzymatic solution:4～7g/ml, preferably 5:6g/ml.
Further, under above-mentioned steps parameter, in order that Snakegourd Fruit seed is preferably separated with flesh, it is biological in a and b step
Enzymatic solution temperature is preferably 30 DEG C；In the b step, time of repose is preferably 65～84h, most preferably 72h；In the step c
Washing times preferably 2 or 3 times.
Further, the purposes of amylase, cellulase in snake gourd fruit pulp is sloughed is also disclosed in the present invention；Preferably,
The amylase is α-amylase；The cellulase is endoglucanase, exoglucanase, in beta glucan glycosides enzyme
It is at least one.
The present invention is realized using biological enzyme hydrolysis principle, using enzyme to the water-disintegrable of the organic principles such as starch, saccharide
Can, make the organic principle in snake gourd fruit pulp be decomposed, so that its flesh seed is separated.
Beneficial effect of the present invention：
1st, compared with the method for traditional eluting snake gourd fruit pulp, the present invention sloughs the method pair of snake gourd fruit pulp using enzyme auxiliary
Snakegourd Fruit seed not damaged, Snakegourd Fruit seed are more complete.
2nd, the method that enzyme auxiliary of the present invention sloughs snake gourd fruit pulp, using enzyme such as amylase, cellulase, by Fructus Trichosanthis
Nutritional labeling in flesh such as saccharide, starch, cellulose etc. decompose, and accelerate degraded, the spoilage rate of snake gourd fruit pulp so that snake gourd fruit pulp
It is easier to be divested, so as to obtain cleaner Snakegourd Fruit seed.
3rd, enzyme auxiliary of the present invention sloughs the method process is simple of snake gourd fruit pulp, with low cost.
4th, the method that enzyme auxiliary of the present invention sloughs snake gourd fruit pulp soaks softening time most needs 3～5 days, compares tradition
12～14 days of method, substantially reduce elution time.
5th, enzyme auxiliary of the present invention is sloughed in the method for snake gourd fruit pulp, and washing times are only 2～3 times, compare traditional method
In washing 6～10 times, both simplified operating procedure, saved the time, and saved again and used water, reduce further and produce into
This.
6th, snake gourd fruit pulp medical value is high, can be used to extract beauty, cosmetics, but traditional elution process time is long, Fructus Trichosanthis
Flesh all rots, it is impossible to recycles, reuses can snake gourd fruit pulp using enzyme process, increased the added value of Fructus Trichosanthis.
Specific embodiment
The method that enzyme auxiliary of the present invention sloughs snake gourd fruit pulp, it is characterised in that comprise the steps:By the Fructus Trichosanthis with flesh
Seed is put in biological enzymatic solution, after Snakegourd Fruit seed is separated with flesh, takes Snakegourd Fruit seed, washing, is dried, is obtained final product Snakegourd Fruit seed.
Wherein, the enzyme in biological enzymatic solution can hydrolyze the organic principle in snake gourd fruit pulp, so as to cause snake gourd fruit pulp and melon
Beach wormwood seed is separated.
Further, effect is sloughed in order to take into account cost and snake gourd fruit pulp, described enzyme auxiliary sloughs the side of snake gourd fruit pulp
Method, comprises the steps：
In a, the biological enzymatic solution that will be put into flesh Snakegourd Fruit seed, wherein, the solid-liquid ratio with flesh Snakegourd Fruit seed with biological enzymatic solution
For 3～8:3～8g/ml, enzyme solution temperature are 25～35 DEG C；
B, 25～35 DEG C of holding enzyme solution temperature, stand 60～120h；
C, Snakegourd Fruit seed is taken, washed, be dried, obtain final product.
Further, in order to reduce production cost, while ensure that snake gourd fruit pulp can be efficiently separated, the biological enzymatic solution
Concentration is preferably 0.05～0.75wt%, most preferably more preferably 0.15～0.65wt%, 0.25wt%.
Further, for improve production efficiency, enzyme of the present invention is adopted in aiding in the method for slough snake gourd fruit pulp
Enzyme be preferably amylase, at least one in cellulase；Further, the amylase is preferably α-amylase；
At least one in the preferred endoglucanase of the cellulase, exoglucanase, beta glucan glycosides enzyme.
Further, enzymatic solution consumption can excessively cause the waste of raw material, and consumption is crossed
Really, in order to accelerate snake gourd fruit pulp decomposition rate, while make the eluting of Snakegourd Fruit seed cleaner, preferably, band flesh melon in a steps
Beach wormwood seed is 4～7 with the solid-liquid ratio of biological enzymatic solution:4～7g/ml, preferably 5:6g/ml.
Further, under above-mentioned steps parameter, in order that Snakegourd Fruit seed is preferably separated with flesh, it is biological in a and b step
Enzymatic solution temperature is preferably 30 DEG C；In the b step, time of repose is preferably 65～84h, most preferably 72h；In the step c
Washing times preferably 2 or 3 times.
Further, the purposes of amylase, cellulase in snake gourd fruit pulp is sloughed is also disclosed in the present invention；Preferably,
The amylase is α-amylase；The cellulase is endoglucanase, exoglucanase, in beta glucan glycosides enzyme
It is at least one.
The present invention is realized using biological enzyme hydrolysis principle, using enzyme to the water-disintegrable of the organic principles such as starch, saccharide
Can, make the organic principle in snake gourd fruit pulp be decomposed, so that its flesh seed is separated.
The specific embodiment of the present invention is further described with reference to embodiment, not therefore by present invention limit
System is among described scope of embodiments.
Embodiment 1 affords Snakegourd Fruit seed using the inventive method
In a, the α-amylase solution that 30g is put into 30ml concentration for 0.75wt% with flesh Snakegourd Fruit seed, wherein, enzymatic solution temperature
Spend for 25 DEG C；
B, 25 DEG C of holding enzymatic solution temperature, stand 60h；
C, Snakegourd Fruit seed is taken, washed, be dried, obtain final product clean Snakegourd Fruit seed.
Embodiment 2 affords Snakegourd Fruit seed using the inventive method
In a, the endo-glucanase enzymatic solution that 30g is put into 80ml concentration for 0.05wt% with flesh Snakegourd Fruit seed, wherein, enzyme is molten
Liquid temp is 35 DEG C；
B, 35 DEG C of holding enzymatic solution temperature, stand 120h；
C, Snakegourd Fruit seed is taken, washed, be dried, obtain final product clean Snakegourd Fruit seed.
Embodiment 3 affords Snakegourd Fruit seed using the inventive method
In a, the enzymatic solution that 50g is put into 60ml concentration for 0.15wt% with flesh Snakegourd Fruit seed, wherein, enzyme in enzymatic solution
It is made up of α-amylase, endoglucanase, exoglucanase, the mixing of beta glucan glycosides enzyme, enzymatic solution temperature is 30 DEG C；
B, 30 DEG C of holding enzymatic solution temperature, stand 72h；
C, Snakegourd Fruit seed is taken, washed, be dried, obtain final product clean Snakegourd Fruit seed.
Embodiment 4 affords Snakegourd Fruit seed using the inventive method
In a, the enzymatic solution that 80g is put into 30ml concentration for 0.65wt% with flesh Snakegourd Fruit seed, wherein, enzyme in enzymatic solution
It is made up of α-amylase, exoglucanase, the mixing of beta glucan glycosides enzyme, enzymatic solution temperature is 30 DEG C；
B, 30 DEG C of holding enzymatic solution temperature, stand 84h；
C, Snakegourd Fruit seed is taken, washed, be dried, obtain final product clean Snakegourd Fruit seed.
Embodiment 5 affords Snakegourd Fruit seed using the inventive method
In a, the enzymatic solution that 40g is put into 70ml concentration for 0.35wt% with flesh Snakegourd Fruit seed, wherein, the biology in enzymatic solution
Enzyme is made up of beta glucan glycosides enzyme and α-amylase, and enzymatic solution temperature is 30 DEG C；
B, 30 DEG C of holding enzymatic solution temperature, stand 72h；
C, Snakegourd Fruit seed is taken, washed, be dried, obtain final product clean Snakegourd Fruit seed.
Embodiment 6 affords Snakegourd Fruit seed using the inventive method
A, by 50g band flesh Snakegourd Fruit seed be put in biological enzymatic solution, wherein, biological enzymatic solution by 25ml concentration be 0.75wt%
α-amylase and the concentration of 25ml mix for the cellulase of 0.25wt%, enzymatic solution temperature is 30 DEG C；Wherein, it is described
Cellulase is exoglucanase, the mixture of beta glucan glycosides enzyme composition；
B, 30 DEG C of holding enzymatic solution temperature, stand 72h；
C, Snakegourd Fruit seed is taken, washed, be dried, obtain final product clean Snakegourd Fruit seed.
Embodiment 7 affords Snakegourd Fruit seed using the inventive method
In a, the α-amylase solution that 70g is put into 40ml concentration for 0.75wt% with flesh Snakegourd Fruit seed, enzymatic solution temperature is 30
℃；
B, 30 DEG C of holding enzymatic solution temperature, stand 72h；
C, Snakegourd Fruit seed is taken, washed, be dried, obtain final product clean Snakegourd Fruit seed.
Embodiment 8 affords Snakegourd Fruit seed using the inventive method
In a, the cellulase solution that 50g is put into 60ml concentration for 0.25wt% with flesh Snakegourd Fruit seed, enzymatic solution temperature is 30
℃；Wherein, the cellulase is endoglucanase, exoglucanase, the mixture of beta glucan glycosides enzyme composition；
B, 30 DEG C of holding enzymatic solution temperature, stand 65h；
C, Snakegourd Fruit seed is taken, washed, be dried, obtain final product clean Snakegourd Fruit seed.
Claims (15)
1. the method that enzyme auxiliary sloughs snake gourd fruit pulp, it is characterised in that comprise the steps:
A, will be put in biological enzymatic solution with flesh Snakegourd Fruit seed, wherein, it is 3～8 with the solid-liquid ratio of biological enzymatic solution with flesh Snakegourd Fruit seed:
3～8g/ml, enzyme solution temperature are 25～35 DEG C；
B, 25～35 DEG C of holding enzyme solution temperature, stand 60～120h；
C, Snakegourd Fruit seed is taken, washed, be dried, obtain final product；
Wherein, the enzyme be amylase, at least one in cellulase.
2. the method that enzyme auxiliary according to claim 1 sloughs snake gourd fruit pulp, it is characterised in that the biological enzymatic solution
Concentration is 0.05～0.75wt%.
3. the method that enzyme auxiliary according to claim 2 sloughs snake gourd fruit pulp, it is characterised in that：The biological enzymatic solution
Concentration is 0.15～0.65wt%.
4. the method that enzyme auxiliary according to claim 2 sloughs snake gourd fruit pulp, it is characterised in that：The biological enzymatic solution
Concentration is 0.25wt%.
5. the method that enzyme auxiliary according to claim 1 sloughs snake gourd fruit pulp, it is characterised in that：The amylase be α-
Amylase；The cellulase is endoglucanase, exoglucanase, at least one in beta glucan glycosides enzyme.
6. the method that the enzyme auxiliary according to any one of Claims 1 to 5 sloughs snake gourd fruit pulp, it is characterised in that：The a
Solid-liquid ratio with flesh Snakegourd Fruit seed and biological enzymatic solution in step is 4～7:4～7g/ml.
7. the method that the enzyme auxiliary according to any one of Claims 1 to 5 sloughs snake gourd fruit pulp, it is characterised in that：The a
Solid-liquid ratio with flesh Snakegourd Fruit seed and biological enzymatic solution in step is 5:6g/ml.
8. the method that the enzyme auxiliary according to any one of Claims 1 to 5 sloughs snake gourd fruit pulp, it is characterised in that：The a
It it is 30 DEG C with enzyme solution temperature in b step；In the b step, time of repose is 65～84h；Wash in the step c secondary
Number is 2 or 3 times.
9. the method that enzyme auxiliary according to claim 6 sloughs snake gourd fruit pulp, it is characterised in that：In a and b step
Enzyme solution temperature is 30 DEG C；In the b step, time of repose is 65～84h；In the step c, washing times are 2 or 3
It is secondary.
10. the method that enzyme auxiliary according to claim 7 sloughs snake gourd fruit pulp, it is characterised in that：The a and b step
Middle enzyme solution temperature is 30 DEG C；In the b step, time of repose is 65～84h；In the step c, washing times are 2 or 3
It is secondary.
The method that the 11. enzyme auxiliary according to any one of Claims 1 to 5 slough snake gourd fruit pulp, it is characterised in that：It is described
In a and b step, enzyme solution temperature is 30 DEG C；In the b step, time of repose is 72h；In the step c, washing times are
2 or 3 times.
The method that 12. enzyme auxiliary according to claim 6 slough snake gourd fruit pulp, it is characterised in that：The a and b step
Middle enzyme solution temperature is 30 DEG C；In the b step, time of repose is 72h；In the step c, washing times are 2 or 3 times.
The method that 13. enzyme auxiliary according to claim 7 slough snake gourd fruit pulp, it is characterised in that：The a and b step
Middle enzyme solution temperature is 30 DEG C；In the b step, time of repose is 72h；In the step c, washing times are 2 or 3 times.
The purposes of 14. amylase, cellulase in snake gourd fruit pulp is sloughed.
15. amylase according to claim 14, purposes of the cellulase in snake gourd fruit pulp is sloughed, it is characterised in that：Institute
Amylase is stated for α-amylase；The cellulase is endoglucanase, exoglucanase, in beta glucan glycosides enzyme extremely
Few one kind.
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| CN105287689B (en) * | 2015-11-13 | 2019-10-18 | 四川师范大学 | The preparation method of Snakegourd Fruit seed |
| CN106233872A (en) * | 2016-08-04 | 2016-12-21 | 安徽徽大农业有限公司 | A kind of cucumber seeds extracting method |
| CN106957928A (en) * | 2017-03-24 | 2017-07-18 | 南京中医药大学 | A kind of preparation method of snakegourd fructose syrup |
| CN107549748A (en) * | 2017-08-12 | 2018-01-09 | 安徽廷龙食品有限公司 | A kind of method for processing Snakegourd Fruit seed |
Citations (1)
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| CN103087548A (en) * | 2013-01-29 | 2013-05-08 | 安阳天尊生物工程有限公司 | Method for extracting trichosanthes kirilowii maxim uranidin |
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| CN103087548A (en) * | 2013-01-29 | 2013-05-08 | 安阳天尊生物工程有限公司 | Method for extracting trichosanthes kirilowii maxim uranidin |
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