CN103636494A - Drought resistance identification method of virus-free potato test-tube seedling - Google Patents
Drought resistance identification method of virus-free potato test-tube seedling Download PDFInfo
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- CN103636494A CN103636494A CN201310592142.7A CN201310592142A CN103636494A CN 103636494 A CN103636494 A CN 103636494A CN 201310592142 A CN201310592142 A CN 201310592142A CN 103636494 A CN103636494 A CN 103636494A
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Abstract
The invention discloses a drought resistance identification method of a virus-free potato test-tube seedling, a multi-layer filter bed liquid culture method is used, a MS (Murashige and Skoog) culture medium is used for culture of the virus-free potato test-tube seedling, and polyethylene glycol is used for gradient water stress, seedling transplant and cultivation. According to the drought resistance identification method, the MS (Murashige and Skoog) culture medium is used for culture of the virus-free potato test-tube seedling, and the polyethylene glycol is used for gradient water stress, seedling transplant and cultivation, so that the drought resistance identification of the virus-free potato test-tube seedling can be quick, convenient and stable.
Description
Technical field
The invention belongs to a kind of authentication method of biological drought resisting, particularly a kind of potato detoxicating cuvette seedling drought resistance appraising method.
Background technology
Soil drought is a global problem, has become the one of the main reasons that damage to crops growth, soil desertification and natural ecological environment worsen.At present, whole world arid biogeographic zone area accounts for 24 % of global area, and China's arid biogeographic zone area surpasses 250 km2 and accounts for the more than 1/4 of area.Mainly be distributed in the Northwest inland area of China, as Hexi Corridor In Gansu, Xinjiang, Chaidamu Basin, Qinghai Province, Ningxia and some areas, the Inner Mongol.China's gross amount of water resources 2.8 hundreds of millions m3, but water source occupancy volume 2300 m3 only per capita, 1/4 of not enough world per capita share, occupies 109, the world, and by the development of World Water resource and environment, federation classifies one of 13 poor-water countries as.According to Boyer (1982) statistics, between 1939 ~ 1978 years, affect output reduction that in the various environmental factors of crop yield, Drought and salt alkali causes up to more than 40 %.Drought and salt alkali becomes a large obstacle of agricultural production steady-state growth.
The research of the aspects such as relation, the drought resistance that Chinese scholars is coerced with other in Evaluation of drought resistance, drought resistance physiological mechanism, anti-drought gene engineering, the drought resisting of potato at present become all obtains certain progress.Evaluation of drought resistance mainly adopt take output as basic drought resistance coefficient be standard, can also carry out in conjunction with the physical signs such as carbon isotope discrimination, morphological index the overall merit of drought resistance in addition.It is particularly important that the Potato Cultivars of therefore, seed selection drought resistance just seems.
Summary of the invention
The object of this invention is to provide a kind of potato detoxicating cuvette seedling drought resistance appraising method, provide a kind of quick, easy, stable potato detoxicating cuvette seedling drought resistance appraising method.
To achieve these goals, the present invention is by the following technical solutions:
A potato detoxicating cuvette seedling drought resistance appraising method, is used multi-layer filter paper bed liquid cultivating method, with liquid MS medium, cultivates potato detoxicating cuvette seedling, and polyethylene glycol (PEG-6000) is done gradient water stress, transplants seedlings, and cultivates.
Described multi-layer filter paper bed liquid cultivating method, the qualitative filter paper that is 0.5m * 0.5m by model is converted into 6 layers, is cut into " ten " font, by inwardly folding 90 °, four edge dotted lines, making size is the filter paper bed of 1.5cm * 5.5cm * 5.5cm, then at 9 of filter paper bed central authorities punchings, aperture d=0.3mm.And with tweezers, its level is put into the blake bottle bottom about 150mL.
Described liquid MS medium is liquid MS medium.
Described polyethylene glycol (PEG-6000) is done gradient water stress, configuration liquid MS medium, the liquid MS medium preparing is divided into five groups, in each group, add respectively polyethylene glycol (PEG-6000), fully dissolve, make containing 0%, 5%, 10%, 15%, five kinds of liquid MS mediums of 20% polyethylene glycol (PEG-6000), then the liquid MS medium of every kind of different polyethylene glycol (PEG-6000) content is poured into respectively in 10 blake bottles, totally 50 blake bottles, in each blake bottle, fill liquid MS medium 50 ml and face lower than filter paper bed surface 0.2cm.
The described blake bottle autoclaving that installs liquid MS medium.
Described transplanting seedlings measured band after drought resistance potato detoxicating cuvette seedling is cut into the stem section with 2 axillalry buds under aseptic condition and moved into respectively in polyethylene glycol (PEG-6000) liquid MS medium of variable concentrations gradient.
In described each blake bottle, inoculate 5 stem sections, sealing.
Described blake bottle after transplanting seedlings is successfully placed on to 22 ℃～28 ℃, under 2000lx～3000lx condition, cultivates, cultivate 30 days.
Described cultivation, after 30 days, is selected untainted blake bottle, measure potato detoxicating cuvette take root situation, be that root is long, test-tube plantlet plant height mensuration, test-tube plantlet blade number.
Beneficial effect of the present invention is: the present invention adopts liquid MS medium to cultivate potato detoxicating cuvette seedling, and polyethylene glycol is done gradient water stress, can be quick, and easy, stable evaluation potato detoxicating cuvette seedling drought resistance.
Embodiment
In order to make object of the present invention, technical scheme and advantage clearer, below in conjunction with embodiment, the present invention is further elaborated.Should be appreciated that specific embodiment described herein, only in order to explain the present invention, is not intended to limit the present invention.
A potato detoxicating cuvette seedling drought resistance appraising method, is characterized in that, uses multi-layer filter paper bed liquid cultivating method, with liquid MS medium, cultivates potato detoxicating cuvette seedling, and polyethylene glycol is done gradient water stress, transplants seedlings, and cultivates.
Multi-layer filter paper bed liquid cultivating method, the qualitative filter paper that is 0.5m * 0.5m by model is converted into 6 layers, is cut into " ten " font, by inwardly folding 90 °, four edge dotted lines, making size is the filter paper bed of 1.5cm * 5.5cm * 5.5cm, then at 9 of filter paper bed central authorities punchings, aperture d=0.3mm.And with tweezers, its level is put into the blake bottle bottom about 150mL.
Liquid MS medium is liquid MS medium.
Polyethylene glycol is done gradient water stress, configuration liquid MS medium, the liquid MS medium preparing is divided into five groups, in each group, add respectively polyethylene glycol, fully dissolve, make five kinds of liquid MS mediums containing 0%, 5%, 10%, 15%, 20% polyethylene glycol, then the liquid MS medium of every kind of different polyethyleneglycol contents is poured into respectively in 10 blake bottles, totally 50 blake bottles, fill liquid MS medium 50 ml and face lower than filter paper bed surface 0.2cm in each blake bottle.
Install the blake bottle autoclaving of liquid MS medium.
Transplant seedlings band is measured after drought resistance potato detoxicating cuvette seedling is cut into the stem section with 2 axillalry buds under aseptic condition and moved into respectively in the polyethylene glycol liquid MS medium of variable concentrations gradient.
In each blake bottle, inoculate 5 stem sections, sealing.
Blake bottle after transplanting seedlings is successfully placed on to 22 ℃～28 ℃, under 2000lx～3000lx condition, cultivates, cultivate 30 days.
Cultivate after 30 days, select untainted blake bottle, measure potato detoxicating cuvette take root situation, be that root is long, test-tube plantlet plant height mensuration, test-tube plantlet blade number.
Although above-mentioned, the specific embodiment of the present invention is described in conjunction with the embodiments; but be not limiting the scope of the invention; one of ordinary skill in the art should be understood that; on the basis of technical scheme of the present invention, those skilled in the art do not need to pay various modifications that performing creative labour can make or distortion still within protection scope of the present invention.
Claims (9)
1. a potato detoxicating cuvette seedling drought resistance appraising method, is characterized in that, uses multi-layer filter paper bed liquid cultivating method, with liquid MS medium, cultivates potato detoxicating cuvette seedling, and polyethylene glycol is done gradient water stress, transplants seedlings, and cultivates.
2. a kind of potato detoxicating cuvette seedling drought resistance appraising method as claimed in claim 1, it is characterized in that: described multi-layer filter paper bed liquid cultivating method, the qualitative filter paper that is 0.5m * 0.5m by model is converted into 6 layers, be cut into " ten " font, by inwardly folding 90 °, four edge dotted lines, making size is the filter paper bed of 1.5cm * 5.5cm * 5.5cm, then 9 of filter paper bed central authorities punchings, aperture d=0.3mm, and with tweezers, its level is put into the blake bottle bottom of 150mL left and right.
3. a kind of potato detoxicating cuvette seedling drought resistance appraising method as claimed in claim 1, is characterized in that: described liquid MS medium is liquid MS medium.
4. a kind of potato detoxicating cuvette seedling drought resistance appraising method as claimed in claim 1, it is characterized in that: described polyethylene glycol is done gradient water stress, configuration liquid MS medium, the liquid MS medium preparing is divided into five groups, in each group, add respectively polyethylene glycol, fully dissolve, make containing 0%, 5%, 10%, 15%, five kinds of liquid MS mediums of 20% polyethylene glycol, then the liquid MS medium of every kind of different polyethyleneglycol contents is poured into respectively in 10 blake bottles, totally 50 blake bottles, in each blake bottle, fill liquid MS medium 50 ml and face lower than filter paper bed surface 0.2cm.
5. a kind of potato detoxicating cuvette seedling drought resistance appraising method as claimed in claim 3, is characterized in that: described in install the blake bottle autoclaving of liquid MS medium.
6. a kind of potato detoxicating cuvette seedling drought resistance appraising method as claimed in claim 1, is characterized in that: described in transplant seedlings band measured after drought resistance potato detoxicating cuvette seedling is cut into the stem section with 2 axillalry buds under aseptic condition and moved into respectively in the polyethylene glycol liquid MS medium of variable concentrations gradient.
7. a kind of potato detoxicating cuvette seedling drought resistance appraising method as claimed in claim 6, is characterized in that: in described each blake bottle, inoculate 5 stem sections, sealing.
8. a kind of potato detoxicating cuvette seedling drought resistance appraising method as claimed in claim 7, is characterized in that: described blake bottle after transplanting seedlings is successfully placed on to 22 ℃～28 ℃, under 2000lx～3000lx condition, cultivates, cultivate 30 days.
9. a kind of potato detoxicating cuvette seedling drought resistance appraising method as claimed in claim 8, it is characterized in that: described cultivation, after 30 days, is selected untainted blake bottle, measure potato detoxicating cuvette take root situation, be that root is long, test-tube plantlet plant height is measured, test-tube plantlet blade number.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201310592142.7A CN103636494A (en) | 2013-11-22 | 2013-11-22 | Drought resistance identification method of virus-free potato test-tube seedling |
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| CN201310592142.7A CN103636494A (en) | 2013-11-22 | 2013-11-22 | Drought resistance identification method of virus-free potato test-tube seedling |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104255449A (en) * | 2014-08-29 | 2015-01-07 | 江苏徐淮地区徐州农业科学研究所 | Indoor auxiliary sweet potato drought tolerance identification method |
| CN105145350A (en) * | 2015-07-22 | 2015-12-16 | 青岛农业大学 | Preparation method and application of PEG culture medium with solid-liquid two phases |
| CN109169263A (en) * | 2018-10-22 | 2019-01-11 | 陈丁龙 | A kind of breeding method of drought resisting potato |
-
2013
- 2013-11-22 CN CN201310592142.7A patent/CN103636494A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104255449A (en) * | 2014-08-29 | 2015-01-07 | 江苏徐淮地区徐州农业科学研究所 | Indoor auxiliary sweet potato drought tolerance identification method |
| CN105145350A (en) * | 2015-07-22 | 2015-12-16 | 青岛农业大学 | Preparation method and application of PEG culture medium with solid-liquid two phases |
| CN109169263A (en) * | 2018-10-22 | 2019-01-11 | 陈丁龙 | A kind of breeding method of drought resisting potato |
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