CN103454425A - Reagent strip for detecting PCT (procalcitonin) and preparation method of reagent strip - Google Patents
Reagent strip for detecting PCT (procalcitonin) and preparation method of reagent strip Download PDFInfo
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- CN103454425A CN103454425A CN2013103813447A CN201310381344A CN103454425A CN 103454425 A CN103454425 A CN 103454425A CN 2013103813447 A CN2013103813447 A CN 2013103813447A CN 201310381344 A CN201310381344 A CN 201310381344A CN 103454425 A CN103454425 A CN 103454425A
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Abstract
The invention discloses an immunochromatographic reagent strip, and particularly relates to an immunochromatographic reagent strip for detecting PCT (procalcitonin) and a preparation method of the reagent strip. A detection line is arranged on immunochromatographic test paper, and is formed by coating of an antibody, the antibody is selected from a mouse anti-human PCT monoclonal antibody, and a quality control line is formed by coating of a sheep anti-mouse IgG polyclonal antibody; the mouse anti-human PCT monoclonal antibody and colloidal gold are prepared into a colloidal gold combined pad. The preparation method of the immunochromatographic reagent strip for detecting the PCT is characterized by comprising the following steps: A, coating a nitrocellulose membrane with a detection line working solution and a quality control line working solution of each antibody, drying the obtained nitrocellulose membrane at a temperature of 36-38 DEG C for 8-12 hours to obtain a chromatographic membrane; B, preparing the mouse anti-human PCT monoclonal antibody and the colloidal gold into the colloidal gold combined pad; and C, sequentially adhering a sample pad, the colloidal gold combined pad, the chromatographic membrane and a water absorbing pad on a rubber plate to prepare the immunochromatographic reagent strip for detecting the PCT.
Description
Technical field
The present invention discloses a kind of immuno-chromatographic test paper strip, is specifically related to immuno-chromatographic test paper strip of a kind of PCT of detection and preparation method thereof.
Background technology
Immune chromatography test paper is that a kind of chromatographic theory that utilizes grades the target detection molecule in liquid to be detected or one-tenth to flow to detection line (T line) and nature controlling line (C line) on test strips, when detection line generation change color illustrates that testing result is positive, otherwise negative, when nature controlling line generation change color, illustrate that testing result is effective, otherwise invalid.
Immune chromatography test paper has advantage very easily when detecting or diagnosing some disease, so it has fully been applied in the detection or diagnostic procedure of various diseases or symptom.Generally, immune chromatography test paper includes following several structure: sample pad, pad, chromatographic film, adsorptive pads, supporting pad, and described sample pad, collaurum pad, chromatographic film and adsorptive pads stick on supporting pad successively; Be disposed with detection line and nature controlling line on described chromatographic film; Its principle of work is: drop to be measured is added on sample pad, and liquid to be measured utilizes chromatographic theory to flow to pad, and in liquid to be measured, the collaurum bond of determinand in pad is combined, and jointly flows to successively detection line and the nature controlling line on chromatographic film with it; Contain the target detection molecule in liquid to be measured, thus the antibody component that target detection molecule, collaurum bond can comprise in detection line be combined and form immune complex, the detection line color changes simultaneously; When liquid to be measured flow to nature controlling line, the antibody component comprised in nature controlling line can be combined with the collaurum bond, thereby the nature controlling line color changes.
PCT (procalcitonin, Procalcitonin) is a kind of protein, and when serious bacterium, fungi, parasitic infection and pyemia and MOFE, its level in blood plasma raises.When autoimmunity, allergy and virus infections, PCT can not raise.The bacterium of local finite infects, slight infection and chronic inflammation can not cause its rising.Bacterial endotoxin has been served as vital effect in Induction Process.
PCT has reflected the active degree of systemic inflammatory response.The factor that affects the PCT level comprises the size of infected organ and type, the kind of bacterium, degree and the immunoreactive situation of inflammation.In addition, PCT just can measure at the large-scale surgical postoperative 1～4d of small number of patients.
The rising of PCT level appears at serious shock, systemic inflammatory response syndrome (SIRS) and multiple organ dysfunction derangement syndrome (MODS), even without bacterium, infects or bacillary focus.But the PCT level is usually less than those patients that bacillary focus is arranged in these cases.May cause and induce from the enteron aisle release cells factor or bacterial translocation.
Summary of the invention
In view of this, the invention provides immuno-chromatographic test paper strip of a kind of PCT of detection and preparation method thereof, there is the advantages such as sampling is easy, easy and simple to handle, reaction is quick, susceptibility is high, high specificity, applicable Site Detection.
For solving above technical matters, technical scheme of the present invention is to adopt a kind of PCT to detect immune chromatography test paper, and this immune chromatography test paper includes sample pad, pad, chromatographic film, adsorptive pads and supporting pad; Described sample pad, collaurum pad, chromatographic film, adsorptive pads are connected with supporting pad successively in order; It is characterized in that: be disposed with detection zone and Quality Control district on chromatographic film, described detection zone is near collaurum pad one end, and the Quality Control district is near adsorptive pads one end; Be provided with detection line in described detection zone, described detection line is to form by mouse-anti people PCT monoclonal antibody is coated; Described Quality Control is provided with nature controlling line in district, and described nature controlling line is to form by the sheep anti-mouse igg polyclonal antibody is coated; Described collaurum pad: mouse-anti people PCT monoclonal antibody and collaurum are prepared into to the collaurum pad.
In described detection line and nature controlling line, the working fluid concentration of each antibody is mouse-anti people PCT monoclonal antibody 1.5-2.5mg/mL, sheep anti-mouse igg polyclonal antibody 1.0-2.0mg/mL.
In described detection line and nature controlling line, the working fluid concentration of each antibody is mouse-anti people PCT monoclonal antibody 2.0mg/mL, sheep anti-mouse igg polyclonal antibody 1.5mg/mL.
The preparation process that described PCT detects immune chromatography test paper is as follows:
A, each antibody detection line working fluid and nature controlling line working fluid are coated with on nitrocellulose filter, the gained nitrocellulose filter under 36-38 ℃ dry 8-12 hour chromatographic film;
B, mouse-anti people PCT monoclonal antibody and collaurum are prepared into to the collaurum pad;
C, the chromatographic film of A and B step gained and collaurum pad are sticked on successively on offset plate and are prepared into described detection PCT immune chromatography test paper according to the order of sample pad, collaurum pad, chromatographic film, adsorptive pads.
The invention has the beneficial effects as follows: immuno-chromatographic test paper strip of a kind of PCT of detection and preparation method thereof, with conventional reagents, compare, have that sampling is easy, easy and simple to handle, reaction fast, high, the high specificity of susceptibility, be applicable to Site Detection reduce the testing staff workload etc. advantage.
The accompanying drawing explanation:
Fig. 1: PCT detection kit test paper structure schematic diagram.
Reference numeral explanation: 1: sample pad; 2: pad; 3: chromatographic film; 4: adsorptive pads 5: detection line (T); 6: nature controlling line (C); 7: supporting pad.
Embodiment
Embodiment:
A, each antibody detection line working fluid and nature controlling line working fluid are coated with on nitrocellulose filter, the gained nitrocellulose filter under 36-38 ℃ dry 8-12 hour chromatographic film;
B, mouse-anti people PCT monoclonal antibody and collaurum are prepared into to the collaurum pad;
C, the chromatographic film of A and B step gained and collaurum pad are sticked on successively on offset plate and are prepared into described detection PCT immune chromatography test paper according to the order of sample pad, collaurum pad, chromatographic film, adsorptive pads.
Claims (4)
1. a PCT detects immune chromatography test paper, and this immune chromatography test paper includes sample pad, pad, chromatographic film, adsorptive pads and supporting pad; Described sample pad, collaurum pad, chromatographic film, adsorptive pads are connected with supporting pad successively in order; It is characterized in that: be disposed with detection zone and Quality Control district on chromatographic film, described detection zone is near collaurum pad one end, and the Quality Control district is near adsorptive pads one end; Be provided with detection line in described detection zone, described detection line is to form by mouse-anti people PCT monoclonal antibody is coated; Described Quality Control is provided with nature controlling line in district, and described nature controlling line is to form by the sheep anti-mouse igg polyclonal antibody is coated; Described collaurum pad: mouse-anti people PCT monoclonal antibody and collaurum are prepared into to the collaurum pad.
2. PCT detects immune chromatography test paper according to claim 1, and it is characterized in that: in described detection line and nature controlling line, the working fluid concentration of each antibody is mouse-anti people PCT monoclonal antibody 1.5-2.5mg/mL, sheep anti-mouse igg polyclonal antibody 1.0-2.0mg/mL.
3. PCT detects immune chromatography test paper according to claim 4, and it is characterized in that: in described detection line and nature controlling line, the working fluid concentration of each antibody is mouse-anti people PCT monoclonal antibody 2.0mg/mL, sheep anti-mouse igg polyclonal antibody 1.5mg/mL.
4. one kind prepares the preparation method that the described PCT of claim 1 detects immune chromatography test paper, it is characterized in that including following steps:
A, each antibody detection line working fluid and nature controlling line working fluid are coated with on nitrocellulose filter, the gained nitrocellulose filter under 36-38 ℃ dry 8-12 hour chromatographic film;
B, mouse-anti people PCT monoclonal antibody and collaurum are prepared into to the collaurum pad;
C, the chromatographic film of A and B step gained and collaurum pad are sticked on successively on offset plate and are prepared into described detection PCT immune chromatography test paper according to the order of sample pad, collaurum pad, chromatographic film, adsorptive pads.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013103813447A CN103454425A (en) | 2013-08-24 | 2013-08-24 | Reagent strip for detecting PCT (procalcitonin) and preparation method of reagent strip |
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| CN2013103813447A CN103454425A (en) | 2013-08-24 | 2013-08-24 | Reagent strip for detecting PCT (procalcitonin) and preparation method of reagent strip |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104090109A (en) * | 2014-07-25 | 2014-10-08 | 胡晓武 | Colloidal gold immunochromatography test paper and colloidal gold immunochromatography test method for quickly detecting human blood procalcitonin |
| CN105467116A (en) * | 2015-11-28 | 2016-04-06 | 宁波美康生物科技股份有限公司 | Convenient procalcitonin detection kit |
| WO2017121001A1 (en) * | 2016-01-16 | 2017-07-20 | 深圳市华科安测信息技术有限公司 | Gold-labeled antibody test strip for use in early-stage diabetic nephropathy test |
| CN107490683A (en) * | 2016-06-09 | 2017-12-19 | 常州博闻迪医药科技有限公司 | A kind of saliva Procalcitonin collaurum detection method |
| CN108896761A (en) * | 2018-06-29 | 2018-11-27 | 广州爱康生物技术有限公司 | The detection card of the luxuriant and rich with fragrance blue shape bacterium of Marni a kind of, detection box and preparation method thereof |
| CN108896760A (en) * | 2018-06-29 | 2018-11-27 | 广州爱康生物技术有限公司 | The cryptococcal detection card of one kind, detection box and preparation method thereof |
| CN115980341A (en) * | 2022-12-26 | 2023-04-18 | 南京珀尔泰生物技术有限公司 | Novel PCT immunochromatography test strip for quantitatively detecting procalcitonin by colloidal gold method and preparation method thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN202794178U (en) * | 2012-08-07 | 2013-03-13 | 天津中新科炬生物制药有限公司 | Fast quantitative immunochromatographic assay kit for procalcitonin |
| CN202814985U (en) * | 2012-06-26 | 2013-03-20 | 南京基蛋生物科技有限公司 | PCT (procalcitonin)/CRP (C-reactive protein) whole-blood test paper tape |
| CN103018456A (en) * | 2012-11-29 | 2013-04-03 | 深圳市伯劳特生物制品有限公司 | Procalcitonin detection test strip and preparation method thereof |
| CN203101401U (en) * | 2012-11-12 | 2013-07-31 | 南京诺尔曼生物技术有限公司 | Procalcitonin whole blood detecting device |
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2013
- 2013-08-24 CN CN2013103813447A patent/CN103454425A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN202814985U (en) * | 2012-06-26 | 2013-03-20 | 南京基蛋生物科技有限公司 | PCT (procalcitonin)/CRP (C-reactive protein) whole-blood test paper tape |
| CN202794178U (en) * | 2012-08-07 | 2013-03-13 | 天津中新科炬生物制药有限公司 | Fast quantitative immunochromatographic assay kit for procalcitonin |
| CN203101401U (en) * | 2012-11-12 | 2013-07-31 | 南京诺尔曼生物技术有限公司 | Procalcitonin whole blood detecting device |
| CN103018456A (en) * | 2012-11-29 | 2013-04-03 | 深圳市伯劳特生物制品有限公司 | Procalcitonin detection test strip and preparation method thereof |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104090109A (en) * | 2014-07-25 | 2014-10-08 | 胡晓武 | Colloidal gold immunochromatography test paper and colloidal gold immunochromatography test method for quickly detecting human blood procalcitonin |
| CN105467116A (en) * | 2015-11-28 | 2016-04-06 | 宁波美康生物科技股份有限公司 | Convenient procalcitonin detection kit |
| WO2017121001A1 (en) * | 2016-01-16 | 2017-07-20 | 深圳市华科安测信息技术有限公司 | Gold-labeled antibody test strip for use in early-stage diabetic nephropathy test |
| CN107490683A (en) * | 2016-06-09 | 2017-12-19 | 常州博闻迪医药科技有限公司 | A kind of saliva Procalcitonin collaurum detection method |
| CN108896761A (en) * | 2018-06-29 | 2018-11-27 | 广州爱康生物技术有限公司 | The detection card of the luxuriant and rich with fragrance blue shape bacterium of Marni a kind of, detection box and preparation method thereof |
| CN108896760A (en) * | 2018-06-29 | 2018-11-27 | 广州爱康生物技术有限公司 | The cryptococcal detection card of one kind, detection box and preparation method thereof |
| CN115980341A (en) * | 2022-12-26 | 2023-04-18 | 南京珀尔泰生物技术有限公司 | Novel PCT immunochromatography test strip for quantitatively detecting procalcitonin by colloidal gold method and preparation method thereof |
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Application publication date: 20131218 |