CN103385368A - Chinese medicine preparation for preventing and treating postpartum hypogalactia of sow and production method thereof - Google Patents
Chinese medicine preparation for preventing and treating postpartum hypogalactia of sow and production method thereof Download PDFInfo
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Abstract
The invention discloses a Chinese medicine preparation for preventing and treating postpartum hypogalactia of sow. The Chinese medicine preparation is mainly prepared from the following raw materials in parts by weight: 30-50 parts of caltrop, 16-24 parts of rhizoma curculiginis, 20-32 parts of suberect spatholobus stem, 8-16 parts of sealwort, 6-12 parts of lance asiabell root, 12-22 parts of folia perillae acutae, 10-20 parts of cottonrose hibiscus and 14-28 parts of caulis aristolochiae manshuriensis; all the components are pretreated and then subjected to solid fermentation by using a mixed culture to obtain the finished product. The product is scientific in formula, rigorous in process and suitable for industrial popularization; the product is capable of effectively treating postpartum hypogalactia of the sow and improving lactation yield of the sow.
Description
Technical field
The present invention relates to the veterinary Chinese medicine field, be specifically related to a kind of Chinese medicine preparation and preparation method who prevents and treats the sow hypogalactia.
Background technology
Hypogalactia refers to that the breast amount in postpartum due to sow malnutrition, deficiency of qi and blood reduces or complete agalasisa, and the traditional Chinese medical science is galactostasis.Breast is the qi and blood life, and the deficiency of blood breast is few, only has the function of transporting and transforming function of the spleen and stomach essence of water and grain normal, and abilityization is given birth to more qi and blood.
The Etiological of sow hypogalactia is that prenatal nutrition is bad, postpartum feed is not enough, causes sow physique thin and weak, insufficiency of vital energy and blood, and the deficiency of blood being difficult of milk is given birth to; Sheng is crossed in antenatal nursing, and hypomotility, so that stagnation of QI-blood, channels and collaterals are not smooth; Get a fright to stagnation of QI and blood etc. and all can cause this disease.
Clinical symptoms: breast amount reduces or agalasisa, and breast is soft to be dwindled, and the cub number of times of sucking the breast increases, and Chang Yitou supports and hits breast and become thin gradually.The hypogalactia of sow often causes piglet disease-resistant power to descend, or forms cad pig, to pig industry, causes very large economic loss.
Exogenous hormone or hormone like substance can promote mammogenesis and manual-induced lactation, but it has certain side effect, even endangers livestock birds health, and it easily residues in animal products, also can damage human body.Seek livestock and poultry nontoxic.The green and health-care fodder additive that the mankind are had no side effect is the important topic in this field.
Fermentation word material refers under artificial condition is controlled, by microorganism, feed is carried out fermentation process, utilize the metabolic activity of microorganism self, ANFs in vegetalitas, animality and mineral nutriment is decomposed or transform, the easier of generation searched for food, digests, absorbs by livestock and poultry, and the feedstuff of nonhazardous effect.
Very large variation has occurred in feedstuff composition after the probio solid state fermentation, and fermentation word material meta contains various active materials, organic acid, alcohols, aromatic hydrocarbon and a large amount of activated probio of tool.Great many of experiments shows that feed quality has obtained obvious improvement after probiotics fermention, and this is mainly reflected in the following aspects: the degraded of (1) some macromolecular substances has improved the utilization rate of feed.(2) in sweat some anti-nutrition because of composition be removed and degrade, produced simultaneously various bioactivators, as various active peptides, oligosaccharides etc.; Some alcohols, aromatic hydrocarbon, the polysaccharose substance that produce after fermentation make feed have various fragrance, sweet taste, have improved the palatability of word material, can increase the livestock and poultry feed intake, improve word material conversion ratio, promote the animal health growth.(3) the improvement effect of probiotics strain to cultivated animals health.4) preferred body probiotics and metabolite thereof have the function of supplementing or strengthening original medicine; (5) Chinese traditional medicine biology can produce new active material after transforming, and brings new health care, prevention or treatment function.
But of a great variety based on bacterial classification, characteristic is different, and the herbal species for the treatment of sow hypogalactia is also many and mix, the zymotechnique of science and parameter are wayward and definite, therefore select suitable bacterial classification kind, determine the inoculum concentration proportioning, the organic assembling Chinese prescription, determine that scientific and reasonable zymotechnique and parameter are study hotspot and the difficult points in additive for microbe feedstuff field.
Summary of the invention
The present invention is intended to solve the difficult problem that prior art exists, and purpose is to provide a kind of Chinese medicine preparation of preventing and treating the sow hypogalactia, and its scientific formulation can effectively be treated the milking sow hypogalactia, increases the sow lactation amount, thereby improves the piglet growth situation.
Another object of the present invention is to provide a kind of preparation method who prevents and treats the Chinese medicine preparation of sow hypogalactia, the method scientific and precise, and the parameter standard, be fit to industrialization promotion.
The technical scheme that realizes above-mentioned purpose is:
A kind of Chinese medicine preparation of preventing and treating the sow hypogalactia, mainly made by the raw material of following weight proportioning: puncture vine 30-50 part, thizoma curculiginis 16-24 part, reticulate millettia 20-32 part, sealwort 8-16 part, lance asiabell root 6-12 part, perilla leaf 12-22 part, cotton rose 10-20 part, caulis aristologhiae manshuriensis 14-28 part;
Concrete preparation method is as follows:
The puncture vine pretreatment: get puncture vine and be crushed to 100 orders, after adding 7-12 times of benzinum refluxing extraction 3h of puncture vine quality, suction filtration, abandon filtrate, gets filter residue in 80 ℃ of oven dry, obtains pretreated puncture vine standby;
The thizoma curculiginis pretreatment: get thizoma curculiginis and be crushed to 100 orders, adding thizoma curculiginis quality 6-10 mass fraction doubly is the ethanol of 60-80%, in 60 ℃ of water-baths, extracts 3 times, each 1h, merge extract, and decompression recycling ethanol obtains the curculigol extracted extract,, in 80 ℃ of oven dry, obtain pretreated thizoma curculiginis standby;
Collecting cells: aspergillus niger, Trichoderma, bacillus subtilis, brewer's yeast are made respectively the seed inclined-plane, 28 ℃ of constant temperature culture 3d obtain each strain culture, each strain culture is made every milliliter with the aseptic sodium chloride solution that mass fraction is 0.9% respectively and is contained bacterium and count the bacteria suspension of 50-100cfu, in 2-8 ℃, saves backup;
Wherein, the mass ratio of each constituent of slant medium of aspergillus niger is, potato: glucose: agar=10:1:1, pH7.0-7.5;
The mass ratio of each constituent of slant medium of Trichoderma is, glucose: starch: peptone: magnesium sulfate: sodium nitrate: diammonium hydrogen phosphate: potassium chloride: agar=15:25:2:1:0.5:2:0.5:4, pH7.0-7.5;
The mass ratio of each constituent of slant medium of bacillus subtilis is, peptone: beef extract: NaCl: agar=10:3:5:2, pH7.0-7.2;
The mass ratio of each constituent of slant medium of brewer's yeast is, potato: glucose: agar=10:1:1, pH7.0-7.5;
Enzymolysis: reticulate millettia, sealwort, lance asiabell root, perilla leaf, cotton rose, caulis aristologhiae manshuriensis are crushed to respectively 100 orders, must mix herb powder after mixing, add 18-22 doubly to mix the distilled water of herb powder quality, after fully dissolving, add the cellulase that mixes herb powder quality 1-2% in 60 ℃ of hydrolysis 16-22min, the boiling water heating 5min enzyme that goes out again, be cooled to room temperature, centrifugal 5min under the 2000r/min rotating speed, get spray-drying after the precipitation Vacuum Concentration, the mixing herb powder that obtains after enzymolysis is standby;
Solid fermentation: in fermentation tank with the mixing herb powder after sucrose 12-24 part, urea 6-10 part, pretreated puncture vine, pretreated thizoma curculiginis, enzymolysis, sterilizing 20min under 121 ℃.Inoculated aspergillus niger bacteria suspension, Trichoderma bacteria suspension, bacillus subtilis bacteria suspension, brewer's yeast bacteria suspension successively under aseptic condition, the inoculation total amount of above-mentioned four kinds of bacteria suspensions is 11-16 part, and the inoculative proportion of each bacteria suspension is, aspergillus niger: Trichoderma: bacillus subtilis: brewer's yeast=8-12:4-7:12-16:6-10; , in 28 ℃ of bottom fermentation 48h, obtain tunning standby;
Tunning extracts: be the alcohol extract 2 times of 70-85% toward adding its quality 8-12 mass fraction doubly in tunning, and 2-3h at every turn, merging filtrate, decompression recycling ethanol, obtain the tunning alcohol-extracted extract,, in 80 ℃ of oven dry, obtains.
Using method of the present invention: add the present invention of the 1%-2% of basal feed quality in basal feed, mix feeding and get final product.
Wherein, aspergillus niger, Trichoderma, bacillus subtilis, brewer's yeast are purchased from Institute of Micro-biology of the Chinese Academy of Sciences.
Each function of traditional Chinese medicinal components of the present invention is specific as follows:
Puncture vine: hot, bitter, tepor.Return Liver Channel.Has flat liver improving eyesight, loose blood, the antipruritic function of being in fashion.Cure mainly hemostasis and gather, breast closes acute mastitis etc.;
Thizoma curculiginis: suffering, heat.Return the kidney,liver,spleen warp.Has kidney-replenishing, strengthening the bones and muscles, the function of wines used as antirheumatic.Special medicine for yang-tonifying temperature kidney, have strong effect to the sexual gland function, promotes digestion, improves a poor appetite;
Reticulate millettia: bitter, sweet, temperature.Return liver, kidney channel.Have and enrich blood, invigorate blood circulation, the function of vein relaxing.Cure mainly internal lesion caused by overexertion, postpartum the deficiency of blood etc.;
Sealwort: sweet, flat.Return spleen, lung, kidney channel.Has boosting qi and nourishing yin, invigorating the spleen, moistening lung, the function of kidney-nourishing.Cure mainly weakness of the spleen and the stomach, asthenia of essence and blood etc.;
Lance asiabell root: sweet, temperature.Has the logical breast of qi-restoratives, Yin nourishing and lung moistening, the function of detumescence and apocenosis.Cure mainly weakness after being ill, hypogalactia etc.;
Perilla leaf: suffering, temperature.Return lung, the spleen channel.Have loose the trembling with fear of inducing sweat, relieving QI stagnancy in the stomach, the function of hemostasis.The promoting the circulation of qi stomach invigorating is arranged, help digest, the heresy of the blood vessels that can fall apart;
Cotton rose: little suffering, cool.Return lung, the heart, Liver Channel.Have clearing heat and detoxicating, detumescence and apocenosis, the function of cooling blood and hemostasis.Cure mainly the diseases such as sore and toxic, acute mastitis;
Caulis aristologhiae manshuriensis: hardship, cold.The thoughts of returning home, small intestine, urinary bladder channel.Has clearing away the heart-fire, diuresis, the function of the lactogenesis of stimulating the menstrual flow.Cure mainly under humid tropics alactation etc.
Prescription of the present invention is scientific and reasonable, traditional Chinese medicinal components is first carried out pretreatment (thick extraction, enzymolysis etc.), make through the compound bacteria solid fermentation again, can effectively extract the active ingredient in Chinese medicine, and reinforcement drug effect, also can produce new active material (as amino acid, enzyme etc.) in fermentation process, bring new health care, prevention or treatment function.Show through zoopery, the present invention can effectively treat the milking sow hypogalactia, promotes lactation amount, thereby improves the upgrowth situation of piglet.
Specific embodiment
The present invention is described further below in conjunction with specific embodiment, to help understanding content of the present invention.
Embodiment 1:
A kind of Chinese medicine preparation of preventing and treating the sow hypogalactia, mainly made by the raw material of following weight proportioning: 34 parts of puncture vines, 19 parts of thizoma curculiginises, 30 parts of reticulate millettias, 14 parts of sealworts, 8 parts of lance asiabell roots, 16 parts of perilla leafs, 15 parts of cotton roses, 26 parts of caulis aristologhiae manshuriensis;
Concrete preparation method is as follows:
The puncture vine pretreatment: get puncture vine and be crushed to 100 orders, after adding 10 times of benzinum refluxing extraction 3h of puncture vine quality, suction filtration, abandon filtrate, gets filter residue in 80 ℃ of oven dry, obtains pretreated puncture vine standby;
The thizoma curculiginis pretreatment: get thizoma curculiginis and be crushed to 100 orders, the mass fraction that adds 9 times of thizoma curculiginis quality is 70% ethanol, in 60 ℃ of water-baths, extracts 3 times, each 1h, merge extract, and decompression recycling ethanol obtains the curculigol extracted extract,, in 80 ℃ of oven dry, obtain pretreated thizoma curculiginis standby;
Collecting cells: aspergillus niger, Trichoderma, bacillus subtilis, brewer's yeast are made respectively the seed inclined-plane, 28 ℃ of constant temperature culture 3d obtain each strain culture, each strain culture is made every milliliter with the aseptic sodium chloride solution that mass fraction is 0.9% respectively and is contained bacterium and count the bacteria suspension of 60-80cfu, in 4 ℃, saves backup;
Wherein, the mass ratio of each constituent of slant medium of aspergillus niger is, potato: glucose: agar=10:1:1, pH7.3;
The mass ratio of each constituent of slant medium of Trichoderma is, glucose: starch: peptone: magnesium sulfate: sodium nitrate: diammonium hydrogen phosphate: potassium chloride=15:25:2:1:0.5:2:0.5, pH7.2;
The mass ratio of each constituent of slant medium of bacillus subtilis is, peptone: beef extract: NaCl: agar=10:3:5:20, pH7.1;
The mass ratio of each constituent of slant medium of brewer's yeast is, potato: glucose: agar=10:1:1, pH7.2;
Enzymolysis: reticulate millettia, sealwort, lance asiabell root, perilla leaf, cotton rose, caulis aristologhiae manshuriensis are crushed to respectively 100 orders, must mix herb powder after mixing, add 20 times of distilled water that mix the herb powder quality, after fully dissolving, add the cellulase that mixes herb powder quality 1.2% in 60 ℃ of hydrolysis 18min, then the boiling water heating 5min enzyme that goes out, be cooled to room temperature, centrifugal 5min under the 2000r/min rotating speed, get spray-drying after the precipitation Vacuum Concentration, and the mixing herb powder that obtains after enzymolysis is standby;
Solid fermentation: in fermentation tank with the mixing herb powder after 22 parts of sucrose, 8 parts, urea, pretreated puncture vine, pretreated thizoma curculiginis, enzymolysis, sterilizing 20min under 121 ℃.Inoculated aspergillus niger bacteria suspension, Trichoderma bacteria suspension, bacillus subtilis bacteria suspension, brewer's yeast bacteria suspension successively under aseptic condition, the inoculation total amount of above-mentioned four kinds of bacteria suspensions is 13 parts, and the inoculative proportion of each bacteria suspension is, aspergillus niger: Trichoderma: bacillus subtilis: brewer's yeast=9:6:14:7; , in 28 ℃ of bottom fermentation 48h, obtain tunning standby;
Tunning extracts: be 75% alcohol extract 2 times toward the mass fraction that adds 10 times of its quality in tunning, and 2.5h at every turn, merging filtrate, decompression recycling ethanol, obtain the tunning alcohol-extracted extract,, in 80 ℃ of oven dry, obtains.
Embodiment 2
A kind of Chinese medicine preparation of preventing and treating the sow hypogalactia, mainly made by the raw material of following weight proportioning: 30 parts of puncture vines, 16 parts of thizoma curculiginises, 20 parts of reticulate millettias, sealwort 8-16 part, 6 parts of lance asiabell roots, 12 parts of perilla leafs, 10 parts of cotton roses, 14 parts of caulis aristologhiae manshuriensis;
Concrete preparation method is as follows:
The puncture vine pretreatment: get puncture vine and be crushed to 100 orders, after adding 7 times of benzinum refluxing extraction 3h of puncture vine quality, suction filtration, abandon filtrate, gets filter residue in 80 ℃ of oven dry, obtains pretreated puncture vine standby;
The thizoma curculiginis pretreatment: get thizoma curculiginis and be crushed to 100 orders, the mass fraction that adds 6 times of thizoma curculiginis quality is 60% ethanol, in 60 ℃ of water-baths, extracts 3 times, each 1h, merge extract, and decompression recycling ethanol obtains the curculigol extracted extract,, in 80 ℃ of oven dry, obtain pretreated thizoma curculiginis standby;
Collecting cells: aspergillus niger, Trichoderma, bacillus subtilis, brewer's yeast are made respectively the seed inclined-plane, 28 ℃ of constant temperature culture 3d obtain each strain culture, each strain culture is made every milliliter with the aseptic sodium chloride solution that mass fraction is 0.9% respectively and is contained bacterium and count the bacteria suspension of 50-70cfu, in 2 ℃, saves backup;
Wherein, the mass ratio of each constituent of slant medium of aspergillus niger is, potato: glucose: agar=10:1:1, pH7.0;
The mass ratio of each constituent of slant medium of Trichoderma is, glucose: starch: peptone: magnesium sulfate: sodium nitrate: diammonium hydrogen phosphate: potassium chloride=15:25:2:1:0.5:2:0.5, pH7.0;
The mass ratio of each constituent of slant medium of bacillus subtilis is, peptone: beef extract: NaCl: agar=10:3:5:20, pH7.0;
The mass ratio of each constituent of slant medium of brewer's yeast is, potato: glucose: agar=10:1:1, pH7.0;
Enzymolysis: reticulate millettia, sealwort, lance asiabell root, perilla leaf, cotton rose, caulis aristologhiae manshuriensis are crushed to respectively 100 orders, must mix herb powder after mixing, add 18 times of distilled water that mix the herb powder quality, after fully dissolving, add the cellulase that mixes herb powder quality 1% in 60 ℃ of hydrolysis 16min, then the boiling water heating 5min enzyme that goes out, be cooled to room temperature, centrifugal 5min under the 2000r/min rotating speed, get spray-drying after the precipitation Vacuum Concentration, and the mixing herb powder that obtains after enzymolysis is standby;
Solid fermentation: in fermentation tank with the mixing herb powder after 12 parts of sucrose, 6 parts, urea, pretreated puncture vine, pretreated thizoma curculiginis, enzymolysis, sterilizing 20min under 121 ℃.Inoculated aspergillus niger bacteria suspension, Trichoderma bacteria suspension, bacillus subtilis bacteria suspension, brewer's yeast bacteria suspension successively under aseptic condition, the inoculation total amount of above-mentioned four kinds of bacteria suspensions is 11 parts, and the inoculative proportion of each bacteria suspension is, aspergillus niger: Trichoderma: bacillus subtilis: brewer's yeast=8:4:12:6; , in 28 ℃ of bottom fermentation 48h, obtain tunning standby;
Tunning extracts: be 70% alcohol extract 2 times toward the mass fraction that adds 8 times of its quality in tunning, and 2h at every turn, merging filtrate, decompression recycling ethanol, obtain the tunning alcohol-extracted extract,, in 80 ℃ of oven dry, obtains.
Embodiment 3
A kind of Chinese medicine preparation of preventing and treating the sow hypogalactia, mainly made by the raw material of following weight proportioning: 50 parts of puncture vines, 24 parts of thizoma curculiginises, 32 parts of reticulate millettias, 16 parts of sealworts, 12 parts of lance asiabell roots, 22 parts of perilla leafs, 20 parts of cotton roses, 28 parts of caulis aristologhiae manshuriensis;
Concrete preparation method is as follows:
The puncture vine pretreatment: get puncture vine and be crushed to 100 orders, after adding 12 times of benzinum refluxing extraction 3h of puncture vine quality, suction filtration, abandon filtrate, gets filter residue in 80 ℃ of oven dry, obtains pretreated puncture vine standby;
The thizoma curculiginis pretreatment: get thizoma curculiginis and be crushed to 100 orders, the mass fraction that adds 10 times of thizoma curculiginis quality is 80% ethanol, in 60 ℃ of water-baths, extracts 3 times, each 1h, merge extract, and decompression recycling ethanol obtains the curculigol extracted extract,, in 80 ℃ of oven dry, obtain pretreated thizoma curculiginis standby;
Collecting cells: aspergillus niger, Trichoderma, bacillus subtilis, brewer's yeast are made respectively the seed inclined-plane, 28 ℃ of constant temperature culture 3d obtain each strain culture, each strain culture is made every milliliter with the aseptic sodium chloride solution that mass fraction is 0.9% respectively and is contained bacterium and count the bacteria suspension of 80-100cfu, in 8 ℃, saves backup;
Wherein, the mass ratio of each constituent of slant medium of aspergillus niger is, potato: glucose: agar=10:1:1, pH7.5;
The mass ratio of each constituent of slant medium of Trichoderma is, glucose: starch: peptone: magnesium sulfate: sodium nitrate: diammonium hydrogen phosphate: potassium chloride=15:25:2:1:0.5:2:0.5, pH7.5;
The mass ratio of each constituent of slant medium of bacillus subtilis is, peptone: beef extract: NaCl: agar=10:3:5:20, pH7.2;
The mass ratio of each constituent of slant medium of brewer's yeast is, potato: glucose: agar=10:1:1, pH7.5;
Enzymolysis: reticulate millettia, sealwort, lance asiabell root, perilla leaf, cotton rose, caulis aristologhiae manshuriensis are crushed to respectively 100 orders, must mix herb powder after mixing, add 22 times of distilled water that mix the herb powder quality, after fully dissolving, add the cellulase that mixes herb powder quality 2% in 60 ℃ of hydrolysis 22min, then the boiling water heating 5min enzyme that goes out, be cooled to room temperature, centrifugal 5min under the 2000r/min rotating speed, get spray-drying after the precipitation Vacuum Concentration, and the mixing herb powder that obtains after enzymolysis is standby;
Solid fermentation: in fermentation tank with the mixing herb powder after 24 parts of sucrose, 10 parts, urea, pretreated puncture vine, pretreated thizoma curculiginis, enzymolysis, sterilizing 20min under 121 ℃.Inoculated aspergillus niger bacteria suspension, Trichoderma bacteria suspension, bacillus subtilis bacteria suspension, brewer's yeast bacteria suspension successively under aseptic condition, the inoculation total amount of above-mentioned four kinds of bacteria suspensions is 16 parts, and the inoculative proportion of each bacteria suspension is, aspergillus niger: Trichoderma: bacillus subtilis: brewer's yeast=12:7:16:10; , in 28 ℃ of bottom fermentation 48h, obtain tunning standby;
Tunning extracts: be 85% alcohol extract 2 times toward the mass fraction that adds 12 times of its quality in tunning, and 3h at every turn, merging filtrate, decompression recycling ethanol, obtain the tunning alcohol-extracted extract,, in 80 ℃ of oven dry, obtains.
Embodiment 4
A kind of Chinese medicine preparation of preventing and treating the sow hypogalactia, mainly made by the raw material of following weight proportioning: 24 parts of puncture vines, 12 parts of thizoma curculiginises, 48 parts of reticulate millettias, 6 parts of sealworts, 15 parts of lance asiabell roots, 10 parts of perilla leafs, 8 parts of cotton roses, 12 parts of caulis aristologhiae manshuriensis;
Concrete preparation method is as follows:
The puncture vine pretreatment: get puncture vine and be crushed to 100 orders, after adding 6 times of benzinum refluxing extraction 3h of puncture vine quality, suction filtration, abandon filtrate, gets filter residue in 80 ℃ of oven dry, obtains pretreated puncture vine standby;
The thizoma curculiginis pretreatment: get thizoma curculiginis and be crushed to 100 orders, the mass fraction that adds 5 times of thizoma curculiginis quality is 50% ethanol, in 60 ℃ of water-baths, extracts 3 times, each 1h, merge extract, and decompression recycling ethanol obtains the curculigol extracted extract,, in 80 ℃ of oven dry, obtain pretreated thizoma curculiginis standby;
Collecting cells: aspergillus niger, Trichoderma, bacillus subtilis, brewer's yeast are made respectively the seed inclined-plane, 28 ℃ of constant temperature culture 3d obtain each strain culture, each strain culture is made every milliliter with the aseptic sodium chloride solution that mass fraction is 0.9% respectively and is contained bacterium and count the bacteria suspension of 50-70cfu, in 2 ℃, saves backup;
Wherein, the mass ratio of each constituent of slant medium of aspergillus niger is, potato: glucose: agar=10:1:1, pH7.0;
The mass ratio of each constituent of slant medium of Trichoderma is, glucose: starch: peptone: magnesium sulfate: sodium nitrate: diammonium hydrogen phosphate: potassium chloride=15:25:2:1:0.5:2:0.5, pH7.1;
The mass ratio of each constituent of slant medium of bacillus subtilis is, peptone: beef extract: NaCl: agar=10:3:5:20, pH7.0;
The mass ratio of each constituent of slant medium of brewer's yeast is, potato: glucose: agar=10:1:1, pH7.0;
Enzymolysis: reticulate millettia, sealwort, lance asiabell root, perilla leaf, cotton rose, caulis aristologhiae manshuriensis are crushed to respectively 100 orders, must mix herb powder after mixing, add 18-22 doubly to mix the distilled water of herb powder quality, after fully dissolving, add the cellulase that mixes herb powder quality 0.8% in 60 ℃ of hydrolysis 12min, then the boiling water heating 5min enzyme that goes out, be cooled to room temperature, centrifugal 5min under the 2000r/min rotating speed, get spray-drying after the precipitation Vacuum Concentration, and the mixing herb powder that obtains after enzymolysis is standby;
Solid fermentation: in fermentation tank with the mixing herb powder after 10 parts of sucrose, 5 parts, urea, pretreated puncture vine, pretreated thizoma curculiginis, enzymolysis, sterilizing 20min under 121 ℃.Inoculated aspergillus niger bacteria suspension, Trichoderma bacteria suspension, bacillus subtilis bacteria suspension, brewer's yeast bacteria suspension successively under aseptic condition, the inoculation total amount of above-mentioned four kinds of bacteria suspensions is 10 parts, and the inoculative proportion of each bacteria suspension is, aspergillus niger: Trichoderma: bacillus subtilis: brewer's yeast=7:3:11:5; , in 28 ℃ of bottom fermentation 48h, obtain tunning standby;
Tunning extracts: be 65% alcohol extract 2 times toward the mass fraction that adds 7 times of its quality in tunning, and 1h at every turn, merging filtrate, decompression recycling ethanol, obtain the tunning alcohol-extracted extract,, in 80 ℃ of oven dry, obtains.
Embodiment 5
A kind of Chinese medicine preparation of preventing and treating the sow hypogalactia, mainly made by the raw material of following weight proportioning: 55 parts of puncture vines, 13 parts of thizoma curculiginises, 37 parts of reticulate millettias, 22 parts of sealworts, 4 parts of lance asiabell roots, 8 parts of perilla leafs, 7 parts of cotton roses, 13 parts of caulis aristologhiae manshuriensis;
Concrete preparation method is as follows:
The puncture vine pretreatment: get puncture vine and be crushed to 100 orders, after adding 13 times of benzinum refluxing extraction 3h of puncture vine quality, suction filtration, abandon filtrate, gets filter residue in 80 ℃ of oven dry, obtains pretreated puncture vine standby;
The thizoma curculiginis pretreatment: get thizoma curculiginis and be crushed to 100 orders, the mass fraction that adds 12 times of thizoma curculiginis quality is 90% ethanol, in 60 ℃ of water-baths, extracts 3 times, each 1h, merge extract, and decompression recycling ethanol obtains the curculigol extracted extract,, in 80 ℃ of oven dry, obtain pretreated thizoma curculiginis standby;
Collecting cells: aspergillus niger, Trichoderma, bacillus subtilis, brewer's yeast are made respectively the seed inclined-plane, 28 ℃ of constant temperature culture 3d obtain each strain culture, each strain culture is made every milliliter with the aseptic sodium chloride solution that mass fraction is 0.9% respectively and is contained bacterium and count the bacteria suspension of 100-120cfu, in 9 ℃, saves backup;
Wherein, the mass ratio of each constituent of slant medium of aspergillus niger is, potato: glucose: agar=10:1:1, pH7.5;
The mass ratio of each constituent of slant medium of Trichoderma is, glucose: starch: peptone: magnesium sulfate: sodium nitrate: diammonium hydrogen phosphate: potassium chloride=15:25:2:1:0.5:2:0.5, pH7.5;
The mass ratio of each constituent of slant medium of bacillus subtilis is, peptone: beef extract: NaCl: agar=10:3:5:20, pH7.3;
The mass ratio of each constituent of slant medium of brewer's yeast is, potato: glucose: agar=10:1:1, pH7.5;
Enzymolysis: reticulate millettia, sealwort, lance asiabell root, perilla leaf, cotton rose, caulis aristologhiae manshuriensis are crushed to respectively 100 orders, must mix herb powder after mixing, add 24 times of distilled water that mix the herb powder quality, after fully dissolving, add the cellulase that mixes herb powder quality 2.2% in 60 ℃ of hydrolysis 30min, then the boiling water heating 5min enzyme that goes out, be cooled to room temperature, centrifugal 5min under the 2000r/min rotating speed, get spray-drying after the precipitation Vacuum Concentration, and the mixing herb powder that obtains after enzymolysis is standby;
Solid fermentation: in fermentation tank with the mixing herb powder after 26 parts of sucrose, 12 parts, urea, pretreated puncture vine, pretreated thizoma curculiginis, enzymolysis, sterilizing 20min under 121 ℃.Inoculated aspergillus niger bacteria suspension, Trichoderma bacteria suspension, bacillus subtilis bacteria suspension, brewer's yeast bacteria suspension successively under aseptic condition, the inoculation total amount of above-mentioned four kinds of bacteria suspensions is 17 parts, and the inoculative proportion of each bacteria suspension is, aspergillus niger: Trichoderma: bacillus subtilis: brewer's yeast=13:8:16:9; , in 28 ℃ of bottom fermentation 48h, obtain tunning standby;
Tunning extracts: be 95% alcohol extract 2 times toward the mass fraction that adds 13 times of its quality in tunning, and 4h at every turn, merging filtrate, decompression recycling ethanol, obtain the tunning alcohol-extracted extract,, in 80 ℃ of oven dry, obtains.
Test example 1
Choose hypogalactia (symptom: breast turgor but milk is few, dysphoria) sow is 120, be divided at random 6 groups, be respectively embodiment 1-5 group, control group, every group 20, wherein the basal diet of the test sow of embodiment 1-5 group adds respectively the finished product that embodiment 1-5 prepares, and the basal diet of control group adds commercial like product (addition is 0.5g/kg).Judgment criteria: cure: the sow lactation amount is normal, and is in good condition; Effectively: the sow lactation amount improves, but milk is still not enough, and state makes moderate progress; Invalid: hypogalactia, state is had the fidgets.Observe result for the treatment of and the record of sow, in Table 1:
The result for the treatment of of table 1 the present invention to the hypogalactia sow
Result of the test shows: the present invention has good result for the treatment of to the sow hypogalactia, and effect significantly is better than control group, wherein especially to implement 1 best results.Wherein the difference between embodiment 1-5 product preparation method is that technological parameter is different, and visible embodiment 1 technique and parameter combinations are best.
Test example 2
Choose health, parity is the close farrowing sow of body condition of 3-6 tire, be divided at random 6 groups, be respectively embodiment 1-5 group, control group, every group 20, wherein the basal diet of the test sow of embodiment 1-5 group adds respectively the finished product that embodiment 1-5 prepares, and the basal diet of control group adds commercial like product (addition is 0.5g/kg).Record weaned piglet weight, piglet net gain, oestrus of sow interval, sow lactation amount.The results are shown in Table 2:
The impact of table 2 the present invention on the milking sow reproductive performance
Result of the test shows: the present invention can effectively promote the sow lactation amount, shortens the oestrus of sow interval.The factors such as the growing state of piglet and sows in lactation health status, lactation amount, nutrition of breast milk component are closely bound up, by test data as can be known the present invention can effectively increase the heavy and net gain of weaned piglet.Wherein, embodiment 1-3 effect significantly is better than embodiment 4-5, control group, and embodiment 1 best results.In sum, the present invention can effectively increase the sow lactation amount, improves sow reproductive performance, thereby promotes the piglet growth situation.
Claims (4)
1. a Chinese medicine preparation of preventing and treating the sow hypogalactia, is characterized in that: mainly by the raw material of following weight proportioning, made: puncture vine 30-50 part, thizoma curculiginis 16-24 part, reticulate millettia 20-32 part, sealwort 8-16 part, lance asiabell root 6-12 part, perilla leaf 12-22 part, cotton rose 10-20 part, caulis aristologhiae manshuriensis 14-28 part.
2. a kind of Chinese medicine preparation of preventing and treating the sow hypogalactia as claimed in claim 1 is characterized in that:
Concrete preparation method is as follows:
The puncture vine pretreatment: get puncture vine and be crushed to 100 orders, after adding 7-12 times of benzinum refluxing extraction 3h of puncture vine quality, suction filtration, abandon filtrate, gets filter residue in 80 ℃ of oven dry, obtains pretreated puncture vine standby;
The thizoma curculiginis pretreatment: get thizoma curculiginis and be crushed to 100 orders, adding thizoma curculiginis quality 6-10 mass fraction doubly is the ethanol of 60-80%, in 60 ℃ of water-baths, extracts 3 times, each 1h, merge extract, and decompression recycling ethanol obtains the curculigol extracted extract,, in 80 ℃ of oven dry, obtain pretreated thizoma curculiginis standby;
Collecting cells: aspergillus niger, Trichoderma, bacillus subtilis, brewer's yeast are made respectively the seed inclined-plane, 28 ℃ of constant temperature culture 3d obtain each strain culture, each strain culture is made every milliliter with the aseptic sodium chloride solution that mass fraction is 0.9% respectively and is contained bacterium and count the bacteria suspension of 50-100cfu, in 2-8 ℃, saves backup;
Wherein, the mass ratio of each constituent of slant medium of aspergillus niger is, potato: glucose: agar=10:1:1, pH7.0-7.5;
The mass ratio of each constituent of slant medium of Trichoderma is, glucose: starch: peptone: magnesium sulfate: sodium nitrate: diammonium hydrogen phosphate: potassium chloride: agar=15:25:2:1:0.5:2:0.5:4, pH7.0-7.5;
The mass ratio of each constituent of slant medium of bacillus subtilis is, peptone: beef extract: NaCl: agar=10:3:5:2, pH7.0-7.2;
The mass ratio of each constituent of slant medium of brewer's yeast is, potato: glucose: agar=10:1:1, pH7.0-7.5;
Enzymolysis: reticulate millettia, sealwort, lance asiabell root, perilla leaf, cotton rose, caulis aristologhiae manshuriensis are crushed to respectively 100 orders, must mix herb powder after mixing, add 18-22 doubly to mix the distilled water of herb powder quality, after fully dissolving, add the cellulase that mixes herb powder quality 1-2% in 60 ℃ of hydrolysis 16-22min, the boiling water heating 5min enzyme that goes out again, be cooled to room temperature, centrifugal 5min under the 2000r/min rotating speed, get spray-drying after the precipitation Vacuum Concentration, the mixing herb powder that obtains after enzymolysis is standby;
Solid fermentation: in fermentation tank with the mixing herb powder after sucrose 12-24 part, urea 6-10 part, pretreated puncture vine, pretreated thizoma curculiginis, enzymolysis, sterilizing 20min under 121 ℃; Inoculated aspergillus niger bacteria suspension, Trichoderma bacteria suspension, bacillus subtilis bacteria suspension, brewer's yeast bacteria suspension successively under aseptic condition, the inoculation total amount of above-mentioned four kinds of bacteria suspensions is 11-16 part, and the inoculative proportion of each bacteria suspension is, aspergillus niger: Trichoderma: bacillus subtilis: brewer's yeast=8-12:4-7:12-16:6-10; , in 28 ℃ of bottom fermentation 48h, obtain tunning standby;
Tunning extracts: be the alcohol extract 2 times of 70-85% toward adding its quality 8-12 mass fraction doubly in tunning, and 2-3h at every turn, merging filtrate, decompression recycling ethanol, obtain the tunning alcohol-extracted extract,, in 80 ℃ of oven dry, obtains.
3. a kind of Chinese medicine preparation of preventing and treating the sow hypogalactia as claimed in claim 1, is characterized in that: mainly by the raw material of following weight proportioning, made: 34 parts of puncture vines, 19 parts of thizoma curculiginises, 30 parts of reticulate millettias, 14 parts of sealworts, 8 parts of lance asiabell roots, 16 parts of perilla leafs, 15 parts of cotton roses, 26 parts of caulis aristologhiae manshuriensis.
4. a kind of Chinese medicine preparation of preventing and treating the sow hypogalactia as claimed in claim 3 is characterized in that:
Concrete preparation method is as follows:
The puncture vine pretreatment: get puncture vine and be crushed to 100 orders, after adding 10 times of benzinum refluxing extraction 3h of puncture vine quality, suction filtration, abandon filtrate, gets filter residue in 80 ℃ of oven dry, obtains pretreated puncture vine standby;
The thizoma curculiginis pretreatment: get thizoma curculiginis and be crushed to 100 orders, the mass fraction that adds 9 times of thizoma curculiginis quality is 70% ethanol, in 60 ℃ of water-baths, extracts 3 times, each 1h, merge extract, and decompression recycling ethanol obtains the curculigol extracted extract,, in 80 ℃ of oven dry, obtain pretreated thizoma curculiginis standby;
Collecting cells: aspergillus niger, Trichoderma, bacillus subtilis, brewer's yeast are made respectively the seed inclined-plane, 28 ℃ of constant temperature culture 3d obtain each strain culture, each strain culture is made every milliliter with the aseptic sodium chloride solution that mass fraction is 0.9% respectively and is contained bacterium and count the bacteria suspension of 60-80cfu, in 4 ℃, saves backup;
Wherein, the mass ratio of each constituent of slant medium of aspergillus niger is, potato: glucose: agar=10:1:1, pH7.3;
The mass ratio of each constituent of slant medium of Trichoderma is, glucose: starch: peptone: magnesium sulfate: sodium nitrate: diammonium hydrogen phosphate: potassium chloride=15:25:2:1:0.5:2:0.5, pH7.2;
The mass ratio of each constituent of slant medium of bacillus subtilis is, peptone: beef extract: NaCl: agar=10:3:5:20, pH7.1;
The mass ratio of each constituent of slant medium of brewer's yeast is, potato: glucose: agar=10:1:1, pH7.2;
Enzymolysis: reticulate millettia, sealwort, lance asiabell root, perilla leaf, cotton rose, caulis aristologhiae manshuriensis are crushed to respectively 100 orders, must mix herb powder after mixing, add 20 times of distilled water that mix the herb powder quality, after fully dissolving, add the cellulase that mixes herb powder quality 1.2% in 60 ℃ of hydrolysis 18min, then the boiling water heating 5min enzyme that goes out, be cooled to room temperature, centrifugal 5min under the 2000r/min rotating speed, get spray-drying after the precipitation Vacuum Concentration, and the mixing herb powder that obtains after enzymolysis is standby;
Solid fermentation: in fermentation tank with the mixing herb powder after 22 parts of sucrose, 8 parts, urea, pretreated puncture vine, pretreated thizoma curculiginis, enzymolysis, sterilizing 20min under 121 ℃; Inoculated aspergillus niger bacteria suspension, Trichoderma bacteria suspension, bacillus subtilis bacteria suspension, brewer's yeast bacteria suspension successively under aseptic condition, the inoculation total amount of above-mentioned four kinds of bacteria suspensions is 13 parts, and the inoculative proportion of each bacteria suspension is, aspergillus niger: Trichoderma: bacillus subtilis: brewer's yeast=9:6:14:7; , in 28 ℃ of bottom fermentation 48h, obtain tunning standby;
Tunning extracts: be 75% alcohol extract 2 times toward the mass fraction that adds 10 times of its quality in tunning, and 2.5h at every turn, merging filtrate, decompression recycling ethanol, obtain the tunning alcohol-extracted extract,, in 80 ℃ of oven dry, obtains.
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| CN114711337A (en) * | 2022-06-07 | 2022-07-08 | 潍坊柯能生物科技有限公司 | Feed additive for lactating sows and preparation method thereof |
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