CN103300262A - Method for degrading and converting aflatoxin by utilizing astaxanthin - Google Patents
Method for degrading and converting aflatoxin by utilizing astaxanthin Download PDFInfo
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- CN103300262A CN103300262A CN2013101855341A CN201310185534A CN103300262A CN 103300262 A CN103300262 A CN 103300262A CN 2013101855341 A CN2013101855341 A CN 2013101855341A CN 201310185534 A CN201310185534 A CN 201310185534A CN 103300262 A CN103300262 A CN 103300262A
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- astaxanthin
- aflatoxin
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Abstract
The name of invention is a method for degrading and converting aflatoxin by utilizing astaxanthin, and belongs to the technical field of organisms. The technical problem to be solved by the invention is to degrade and convert the aflatoxin in peanut oil and peanut kernel by utilizing the astaxanthin (ester). The main points of the technical scheme are that the astaxanthin ester is extracted from haematococcus pluvialis; the astaxanthin is obtained by saponification; the content of the aflatoxin in the peanut oil and the peanut kernel is effectively reduced by utilizing the astaxanthin and the astaxanthin ester.
Description
Technical field
The present invention relates to biological technical field, is to utilize astaxanthin that the aflatoxin in peanut oil and the shelled peanut is degraded, transformed specifically.
Background technology
Aflatoxin (AF) is the toxicity secondary metabolite that the class that mainly produced by fungi aspergillus flavus (Aspergillus flavus) and aspergillus parasiticus (A.parasiticus) has hyperfluorescenceCeng Yongminggaoyingguang and high immune adsorptivity, has carcinogenic, teratogenesis.Fixed aflatoxin kind has 18 kinds more than in the world wide at present, wherein AFB
1Toxicity the highest, harm the most serious.Aflatoxin threatens maximum to mammal is especially human, and the aflatoxin stable in properties, in case pollute, is difficult to eliminate, so the strict regulation of limiting the quantity of has been made to import and export food, consumption material by countries in the world and International Trade Organization.
Astaxanthin (astaxanthin) belongs to a Carotenoids, is shellfish, such as the main pigment of shrimp, crab, salmon and salmon trout.Contain hydroxyl and carbonyl at ionone ring, make astaxanthin have very strong anti-oxidation function, can remove the free radical that is produced by the ultraviolet ray irradiation in the body, regulate and reduce these injuries that caused by photochemistry; Astaxanthin also has antitumaous effect, strengthens immunization etc., more and more has been subject to scientist's attention.At present about research and the patent of astaxanthin, mainly concentrate on the aspects such as the biosynthesis (be such as application number 200610154678.0 patent), chemical synthesis (be such as application number 200610050107.2 patent) of astaxanthin and the health food of astaxanthin-containing (be such as application number 200710068503.2 patent).We chance on astaxanthin also has extraordinary degraded, transformation to the aflatoxin in the peanut oil, and studies in great detail.
Summary of the invention
The technical problem that the present invention solves is that a kind of method with aflatoxin in astaxanthin degraded, conversion peanut oil and the shelled peanut is provided.
Technical scheme of the present invention is as follows:
The astaxanthin ester that the present invention is used is to extract (mainly containing astaxanthin ester in the haematococcus pluvialis) from haematococcus pluvialis.Concrete grammar is as follows: through the haematococcus pluvialis prothenchyma (of wood) after the astaxanthin induction experiment, and the centrifugal 15min of 4000r/min, precipitation is with 30% methyl alcohol and 65 ℃ of lower 15min that process of 5% potassium hydroxide mixed liquor, to remove chlorophyll, distillation washing 2 times.Through behind the homogeneous under the room temperature (pressure is 40MPa), adopt the astaxanthin of chloroform recovery cell inner accumulation, the extract that obtains concentrates with vacuum rotary evaporator under the lucifuge condition.
The astaxanthin that the present invention is used is that the astaxanthin ester saponification obtains.Concrete grammar is as follows: add 40g/L KOH-methyl alcohol saponification liquor in the astaxanthin ester, carry out saponification 10min under magnetic agitation speed 1000r/min, 15 ℃.Then stop to stir, in cold compartment of refrigerator, leave standstill about 3h, until coloured chloroform is clarified mutually fully, separatory, collect the coloured chloroform phase of lower floor.Extract concentrates with vacuum rotary evaporator under the lucifuge condition, obtains the high-purity astaxanthin.
The AFB in the peanut oil can effectively be degraded, be transformed to described astaxanthin
1, optimum quantum of utilization is 1mg/mL.
The AFB in the shelled peanut can effectively be degraded, be transformed to described astaxanthin ester
1, optimum quantum of utilization is 1mg/g.
The specific embodiment
AFB in the degraded of embodiment 1 astaxanthin, the conversion peanut oil
1Concrete experimental procedure is as follows:
Peanut oil (contains AFB
1100ng/mL), add respectively astaxanthin, make the astaxanthin final concentration be respectively 0mg/mL, 0.5mg/mL, 1mg/mL, 2mg/mL and 3mg/mL, 25 ℃ of lower storages 3 days of lucifuge.The AFB of each experimental group of high-pressure liquid phase chromatogram therapy determining
1Content.Experimental result sees Table 1.
Experimental result in table 1 peanut oil
AFB in the degraded of embodiment 2 astaxanthin esters, the conversion shelled peanut
1Concrete experimental procedure is as follows:
With the peanut of size, quality homogeneous, behind 0.1% clorox immersion 1min,, natural air drying clean with flowing water are removed broken skin, shrivelled peanut.Add AFB in the shelled peanut
1, making its final concentration is 100 μ g/kg.Shelled peanut is divided into four groups, adds respectively astaxanthin ester, make astaxanthin ester concentration be respectively 0mg/g, 0.5mg/g, 1mg/g and 2mg/g, 25 ℃ of lower storages 3 days of lucifuge.The AFB of each experimental group of high-pressure liquid phase chromatogram therapy determining
1Content.Experimental result sees Table 2.
Experimental result in table 2 shelled peanut
Claims (3)
1. the invention is characterized in, utilize astaxanthin that the aflatoxin in peanut oil, the shelled peanut is degraded, transformed.
2. described according to claim 1, utilize the astaxanthin degraded, transform the AFB in the peanut oil
1, optimum quantum of utilization is 1mg/mL.
3. described according to claim 1, utilize astaxanthin (ester) degraded, transform the AFB in the shelled peanut
1, optimum quantum of utilization is 1mg/g.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013101855341A CN103300262A (en) | 2013-05-06 | 2013-05-06 | Method for degrading and converting aflatoxin by utilizing astaxanthin |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013101855341A CN103300262A (en) | 2013-05-06 | 2013-05-06 | Method for degrading and converting aflatoxin by utilizing astaxanthin |
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| CN103300262A true CN103300262A (en) | 2013-09-18 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2013101855341A Pending CN103300262A (en) | 2013-05-06 | 2013-05-06 | Method for degrading and converting aflatoxin by utilizing astaxanthin |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103750084A (en) * | 2013-12-30 | 2014-04-30 | 中国农业科学院农产品加工研究所 | Method for degrading aflatoxin B1 in peanuts |
| CN104804865A (en) * | 2015-04-29 | 2015-07-29 | 淄博群恒油脂有限公司 | First cold-pressing high-yield production technology for oxidation-resistant peanut oil |
| CN105029514A (en) * | 2015-06-16 | 2015-11-11 | 浙江海洋学院 | Modifier capable of reducing saxitox |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101617828A (en) * | 2009-06-14 | 2010-01-06 | 海南大学 | Method for preparing astaxanthin food |
| CN101632420A (en) * | 2009-08-05 | 2010-01-27 | 山东省花生研究所 | Method for degrading aflatoxin in peanut kernels |
-
2013
- 2013-05-06 CN CN2013101855341A patent/CN103300262A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101617828A (en) * | 2009-06-14 | 2010-01-06 | 海南大学 | Method for preparing astaxanthin food |
| CN101632420A (en) * | 2009-08-05 | 2010-01-27 | 山东省花生研究所 | Method for degrading aflatoxin in peanut kernels |
Non-Patent Citations (2)
| Title |
|---|
| 何四旺等: "霉菌及霉菌毒素对畜牧业的影响", 《中国禽业导刊》, vol. 18, no. 17, 30 September 2001 (2001-09-30), pages 8 - 9 * |
| 张晓丽等: "虾青素的抗氧化性及其在营养和医药应用方面的研究", 《食品科学》, vol. 27, no. 1, 31 January 2006 (2006-01-31) * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103750084A (en) * | 2013-12-30 | 2014-04-30 | 中国农业科学院农产品加工研究所 | Method for degrading aflatoxin B1 in peanuts |
| CN103750084B (en) * | 2013-12-30 | 2015-12-02 | 中国农业科学院农产品加工研究所 | One cultivates peanut middle aflatoxin degradation B 1method |
| CN104804865A (en) * | 2015-04-29 | 2015-07-29 | 淄博群恒油脂有限公司 | First cold-pressing high-yield production technology for oxidation-resistant peanut oil |
| CN104804865B (en) * | 2015-04-29 | 2018-10-12 | 淄博群恒油脂有限公司 | A kind of production technology of the anti-oxidant peanut oil of level-one cold press high yield |
| CN105029514A (en) * | 2015-06-16 | 2015-11-11 | 浙江海洋学院 | Modifier capable of reducing saxitox |
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Application publication date: 20130918 |