CN103245788A - 一种用于b细胞抗原表位筛查的多肽阵列合成技术 - Google Patents
一种用于b细胞抗原表位筛查的多肽阵列合成技术 Download PDFInfo
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Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN106383225A (zh) * | 2016-09-14 | 2017-02-08 | 南京医科大学 | 一种蛋白印记膜抗体的洗脱液及其制备方法与应用 |
CN106546580A (zh) * | 2015-09-16 | 2017-03-29 | 北京博肽未名生物技术有限公司 | 一种用于鉴别人参产地的多肽微阵列芯片 |
CN108663522A (zh) * | 2018-05-30 | 2018-10-16 | 河北翰林生物科技有限公司 | 膀胱癌检测试剂盒 |
WO2021121389A1 (en) * | 2019-12-19 | 2021-06-24 | Nanjing GenScript Biotech Co., Ltd. | Circuit with bidirectional current source for generating acidic or basic microenvironment in solution |
CN114181915A (zh) * | 2021-08-30 | 2022-03-15 | 首都医科大学附属北京口腔医院 | 基于kdm2b序列合成的生物活性多肽在间充质干细胞神经分化和再生修复中的应用 |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1997042507A1 (en) * | 1996-05-02 | 1997-11-13 | Isis Innovation Limited | Peptide array and method |
US20070122841A1 (en) * | 2005-11-30 | 2007-05-31 | Rajasekaran John J | Massively parallel synthesis of proteinaceous biomolecules |
US20080161202A1 (en) * | 2006-12-29 | 2008-07-03 | Edelmira Cabezas | Novel strategy for selective regulation of background surface property in microarray fabrication and method to eliminated self quenching in micro arrays |
WO2008151146A2 (en) * | 2007-06-01 | 2008-12-11 | Digitab, Inc. | Peptide arrays and methods of use |
WO2010052939A1 (ja) * | 2008-11-10 | 2010-05-14 | 国立大学法人名古屋大学 | アレルゲンのエピトープ又はその候補の検出方法及びその利用 |
-
2013
- 2013-03-22 CN CN201310093099.XA patent/CN103245788B/zh active Active
Patent Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1997042507A1 (en) * | 1996-05-02 | 1997-11-13 | Isis Innovation Limited | Peptide array and method |
US20070122841A1 (en) * | 2005-11-30 | 2007-05-31 | Rajasekaran John J | Massively parallel synthesis of proteinaceous biomolecules |
US20080161202A1 (en) * | 2006-12-29 | 2008-07-03 | Edelmira Cabezas | Novel strategy for selective regulation of background surface property in microarray fabrication and method to eliminated self quenching in micro arrays |
WO2008151146A2 (en) * | 2007-06-01 | 2008-12-11 | Digitab, Inc. | Peptide arrays and methods of use |
WO2008151146A3 (en) * | 2007-06-01 | 2009-02-26 | Digitab Inc | Peptide arrays and methods of use |
WO2010052939A1 (ja) * | 2008-11-10 | 2010-05-14 | 国立大学法人名古屋大学 | アレルゲンのエピトープ又はその候補の検出方法及びその利用 |
Non-Patent Citations (3)
Title |
---|
A. KRAMER ET AL: "Spot synthesis: observations", 《THE JOURNAL OF PEPTIDE RESEARCH》 * |
RONALD FRANK: "The SPOT-synthesis technique Synthetic peptide arrays on membrane supports—principles and applications", 《JOURNAL OF IMMUNOLOGICAL METHODS》 * |
ULRICH REINEKE ET AL: "Applications of peptide arrays prepared by the SPOT-technology", 《CURRENT OPINION IN BIOTECHNOLOGY》 * |
Cited By (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN106546580A (zh) * | 2015-09-16 | 2017-03-29 | 北京博肽未名生物技术有限公司 | 一种用于鉴别人参产地的多肽微阵列芯片 |
CN106546580B (zh) * | 2015-09-16 | 2019-06-28 | 北京博肽未名生物技术有限公司 | 一种用于鉴别人参产地的多肽微阵列芯片 |
CN106383225A (zh) * | 2016-09-14 | 2017-02-08 | 南京医科大学 | 一种蛋白印记膜抗体的洗脱液及其制备方法与应用 |
CN106383225B (zh) * | 2016-09-14 | 2018-03-13 | 南京医科大学 | 一种蛋白印记膜抗体的洗脱液及其制备方法与应用 |
CN108663522A (zh) * | 2018-05-30 | 2018-10-16 | 河北翰林生物科技有限公司 | 膀胱癌检测试剂盒 |
CN108663522B (zh) * | 2018-05-30 | 2021-05-11 | 河北翰林生物科技有限公司 | 膀胱癌检测试剂盒 |
WO2021121389A1 (en) * | 2019-12-19 | 2021-06-24 | Nanjing GenScript Biotech Co., Ltd. | Circuit with bidirectional current source for generating acidic or basic microenvironment in solution |
CN114181915A (zh) * | 2021-08-30 | 2022-03-15 | 首都医科大学附属北京口腔医院 | 基于kdm2b序列合成的生物活性多肽在间充质干细胞神经分化和再生修复中的应用 |
CN114181915B (zh) * | 2021-08-30 | 2024-01-26 | 首都医科大学附属北京口腔医院 | 基于kdm2b序列合成的生物活性多肽在间充质干细胞神经分化和再生修复中的应用 |
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