CN102167737B - 与猪免疫性状相关的蛋白及其编码基因和应用 - Google Patents
与猪免疫性状相关的蛋白及其编码基因和应用 Download PDFInfo
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Abstract
Description
标签 | 残基 | 序列 |
Poly-Arg | 5-6(通常为5个) | RRRRR |
Poly-His | 2-10(通常为6个) | HHHHHH |
FLAG | 8 | DYKDDDDK |
Strep-tag II | 8 | WSHPQFEK |
c-myc | 10 | EQKLISEEDL |
组分 | 体积 | 终浓度 |
总RNA | 10μL | 2μg |
oligo d(T)11 | 5μL | 1μmol/L |
DEPC H2O | 20μL | - |
5×PCR buffer | 10μL | |
dNTP | 2.5μL | 500μmol/L |
RNAsin | 1μL | 40U |
M-MLV | 1.5μL | 300U |
总体积 | 50μL |
组分 | 体积 | 终浓度 |
dd H2O | 13.2μL | - |
10×PCR buffer | 2μL | |
MgCl2 | 1.2μL | 1.5mmol/L |
引物 | 1μL | 10μmol/L |
dNTP | 0.4μL | 10mmol/L |
Taq DNA聚合酶 | 0.2μL | 1U |
cDNA | 1μL | >100ng |
总体积 | 20μL |
基因型 | 样本个数 | WBC | HGB | MCHC |
GG | 115 | 24.5822±1.9500A | 52.3324±4.3175A | 143.9843±11.4970A |
GT | 19 | 37.8163±4.5654B | 80.3858±10.1083B | 215.9394±26.6979B |
P value | 134 | 0.0058 | 0.0082 | 0.0101 |
Claims (10)
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CN102827835A (zh) * | 2012-05-25 | 2012-12-19 | 东北农业大学 | 一种猪免疫力分子遗传标记及其应用 |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1775255A (zh) * | 2004-11-16 | 2006-05-24 | 唐中林 | 一种治疗***炎的外用药品 |
CN101130573A (zh) * | 2007-09-05 | 2008-02-27 | 中国农业科学院北京畜牧兽医研究所 | 一种猪免疫性状相关蛋白及其编码基因与应用 |
CN101230391A (zh) * | 2007-11-07 | 2008-07-30 | 中国农业科学院北京畜牧兽医研究所 | 一种检测猪肉质性状的方法 |
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Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1775255A (zh) * | 2004-11-16 | 2006-05-24 | 唐中林 | 一种治疗***炎的外用药品 |
CN101130573A (zh) * | 2007-09-05 | 2008-02-27 | 中国农业科学院北京畜牧兽医研究所 | 一种猪免疫性状相关蛋白及其编码基因与应用 |
CN101230391A (zh) * | 2007-11-07 | 2008-07-30 | 中国农业科学院北京畜牧兽医研究所 | 一种检测猪肉质性状的方法 |
Non-Patent Citations (2)
Title |
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CAC39696.1;Genbank;《NCBI》;20010530;全文 * |
Genbank.CAC39696.1.《NCBI》.2001, |
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