CN102086199B - Method for self-extracting recycled riboflavin from liquid waste - Google Patents
Method for self-extracting recycled riboflavin from liquid waste Download PDFInfo
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- CN102086199B CN102086199B CN2010106087532A CN201010608753A CN102086199B CN 102086199 B CN102086199 B CN 102086199B CN 2010106087532 A CN2010106087532 A CN 2010106087532A CN 201010608753 A CN201010608753 A CN 201010608753A CN 102086199 B CN102086199 B CN 102086199B
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Abstract
The invention discloses a method for self-extracting recycled riboflavin from liquid waste. The method adopting an inoculation and sedimentation fermentation method comprises the following steps of: (1) adjusting pH of extracted liquid waste to be 5.0-6.5, and adjusting the temperature to 25-35 DEG C; (2) adding seed liquid to the extracted liquid waste; (3) standing, sedimenting and fermenting for 24-60h; (4) when the titer of the riboflavin in the supernatant is between 100ug/ml and 300ug/ml, discharging the supernatant to obtain a riboflavin-rich deposit; and (5) measuring the titer of the riboflavin in the deposit, doping the deposit into fermentation liquor according to the titer of the riboflavin in the deposit, and extracting the riboflavin in the deposit, or extracting the riboflavin singly according to the extraction method of the fermentation liquor. By adopting the method, the extraction yield of the riboflavin can be improved by about 8-10% through recycling treatment of the liquid wastes, and the total industrial production cost is lowered correspondingly.
Description
Technical field
The present invention relates to a kind of from extracting the method that reclaims vitamin G in the waste liquid.
Background technology
Fermentative Production vitamin G (VB
2), the method for extraction is a lot, and at home in the industrialized production, using more at present is alkali solution technique and acid pasting, or according to the difference of range of product, the method that adopts alkali solution technique and acid pasting to combine is produced.No matter take any process for extracting; The capital produces a large amount of production waste liquids (various mother liquor); These produce the influence of waste liquid because of bulk fermentation residue with other fermentating metabolism product of existence; Or because of the difference of the pH value of waste liquid, residual a large amount of vitamin G, its residual quantity exceeds the solubleness of vitamin G in water far away.Thereby waste liquid can have a strong impact on extract yield, and then cause production cost to increase without reclaiming directly discharging, has a strong impact on follow-up wastewater treatment efficiency.The difficulty that from extract waste liquid, reclaims vitamin G is bigger, and traditional way is that the extraction waste liquid is staticly settled, and then bed mud is reclaimed vitamin G with alkali solution technique.This method ST is long, and efficient is low, can not reclaim dissolved vitamin G (vitamin G is on average tired about 1000ug/ml) in the waste liquid, and contain a lot of organic impuritys in the bed mud, influences vitamin G extract yield and quality product.
Summary of the invention
The purpose of this invention is to provide a kind of method that from extract waste liquid, reclaims vitamin G, improve extract yield, reduce and extract production cost, alleviate the WWT difficulty of extracting waste liquid.
Technical scheme of the present invention is: extract the method that reclaims vitamin G in the waste liquid certainly, take to inoculate the sedimentation fermentation method, its step is following:
(1) will extract pH regulator to 5.0 ~ 6.5 of waste liquid, temperature regulation to 25 ~ 35 ℃;
(2) in extracting waste liquid, add kind of a liquid, planting liquid is the fermented liquid without deactivation that obtains behind the riboflavin fermentation;
(3) with postvaccinal extraction waste liquid standing sedimentation fermentation 24 ~ 60 hours;
(4) in the upper clear supernate vitamin G tire between 100 ~ 300ug/ml the time, emit upper clear supernate, obtain being rich in the sedimentation material of vitamin G;
(5) vitamin G is tired in the measurement sediment, tires according to vitamin G in the sediment, and sediment is blended in the fermented liquid, extracts vitamin G wherein, or extracts vitamin G separately by the process for extracting of fermented liquid.
Middle pH to 5.0 ~ 6.5 with hydrochloric acid, sulfuric acid or NaOH adjusting extraction waste liquid of said step (1), the temperature of using the method adjusting extraction waste liquid that heats up or lower the temperature is at 25 ~ 35 ℃.
The volume that adds kind of liquid in the said step (2) is for extracting 8 ~ 10% of waste liquid volume.
The invention has the beneficial effects as follows: the present invention utilizes mikrobe to extracting the degraded and the utilization of organic nutrient substance in the waste liquid, reduces the solubleness of solution to vitamin G, makes to reclaim that most of vitamin G settles down in the liquid, reaches the purpose that reclaims vitamin G.The present invention has improved the extract yield of vitamin G, and through the recycling of waste liquid, extract yield can improve about 8% ~ 10%, has correspondingly reduced the suitability for industrialized production total cost.While makes and extracts organism and other foster part utilization fully again in the waste liquid owing to inoculate the sedimentation fermentation, greatly reduces the WWT difficulty.
Embodiment
Embodiment 1
In extracting waste liquid, reclaim the method for vitamin G, its step is following:
(1) will extract waste liquid (alkali dissolves mother liquor, oxidizing and crystallizing mother liquor and filtrated stock) and put into settling bowl, under blasting action, with hydrochloric acid adjusting pH to 5.0 ~ 6.0, the temperature of using the method adjusting extraction waste liquid that heats up or lower the temperature is at 25 ~ 30 ℃;
(2) in extracting waste liquid, add kind of a liquid; Planting liquid is the fermented liquid without deactivation that obtains behind the riboflavin fermentation; The volume that adds kind of liquid is for extracting 8% of waste liquid volume; During inoculation, can inoculate, can guarantee to extract the fastest settling velocity and the best recovery of vitamin G in the waste liquid through using by turns of several settling tanks;
(3) with postvaccinal extraction waste liquid standing sedimentation fermentation 24 ~ 60 hours;
(4) extract the waste liquid standing for fermentation after 24 hours; Upper clear supernate appearance was got at every interval in 4 hours; Measure its vitamin G and tire, the tiring when 300ug/ml of vitamin G in the upper clear supernate emits upper clear supernate; Obtain being rich in the sedimentation material of vitamin G, vitamin G tires at 10000 ~ 20000ug/ml in the sediment;
(5) vitamin G is tired in the measurement sediment, tires according to vitamin G in the sediment, and sediment is blended in the fermented liquid, extracts vitamin G wherein.
Embodiment 2
In extracting waste liquid, reclaim the method for vitamin G, its step is following:
(1) will extract waste liquid (alkali dissolves mother liquor, oxidizing and crystallizing mother liquor and filtrated stock) and put into settling bowl, with NaOH adjusting pH to 6.0 ~ 6.5, the temperature of using the method adjusting extraction waste liquid that heats up or lower the temperature is at 30 ~ 35 ℃;
(2) in extracting waste liquid, add kind of a liquid, planting liquid is the fermented liquid without deactivation that obtains behind the riboflavin fermentation, and the volume that adds kind of liquid is for extracting 10% of waste liquid volume;
(3) with postvaccinal extraction waste liquid standing sedimentation fermentation 24 ~ 60 hours;
(4) extract the waste liquid standing for fermentation after 24 hours; Upper clear supernate appearance was got at every interval in 4 hours; Measure its vitamin G and tire, in the upper clear supernate vitamin G tire between 100ug/ml the time, emit upper clear supernate; Obtain being rich in the sedimentation material of vitamin G, vitamin G tires at 10000 ~ 20000ug/ml in the sediment;
(5) process for extracting by fermented liquid extracts vitamin G separately.
Claims (3)
1. extract the method that reclaims vitamin G in the waste liquid certainly, it is characterized in that taking to inoculate the sedimentation fermentation method, its step is following:
(1) will extract pH regulator to 5.0 ~ 6.5 of waste liquid, temperature regulation to 25 ~ 35 ℃;
(2) in extracting waste liquid, add kind of a liquid, planting liquid is the fermented liquid without deactivation that obtains behind the riboflavin fermentation;
(3) with postvaccinal extraction waste liquid standing sedimentation fermentation 24 ~ 60 hours;
(4) in the upper clear supernate vitamin G tire between 100 ~ 300 μ g/ml the time, emit upper clear supernate, obtain being rich in the sedimentation material of vitamin G;
(5) vitamin G is tired in the measurement sediment, tires according to vitamin G in the sediment, and sediment is blended in the fermented liquid, extracts vitamin G wherein, or extracts vitamin G separately by the process for extracting of fermented liquid.
2. according to claim 1 from extracting the method that reclaims vitamin G in the waste liquid; It is characterized in that: middle pH to 5.0 ~ 6.5 with hydrochloric acid, sulfuric acid or NaOH adjusting extraction waste liquid of said step (1), the temperature of using the method adjusting extraction waste liquid that heats up or lower the temperature is at 25 ~ 35 ℃.
3. according to claim 1 from extracting the method that reclaims vitamin G in the waste liquid, it is characterized in that: the volume that adds kind of liquid in the said step (2) is for extracting 8 ~ 10% of waste liquid volume.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN2010106087532A CN102086199B (en) | 2010-12-28 | 2010-12-28 | Method for self-extracting recycled riboflavin from liquid waste |
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| Application Number | Priority Date | Filing Date | Title |
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| CN2010106087532A CN102086199B (en) | 2010-12-28 | 2010-12-28 | Method for self-extracting recycled riboflavin from liquid waste |
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| Publication Number | Publication Date |
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| CN102086199A CN102086199A (en) | 2011-06-08 |
| CN102086199B true CN102086199B (en) | 2012-11-14 |
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Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102670624B (en) * | 2011-03-14 | 2015-04-15 | 福建省医学科学研究院 | Patch for treating rheumatic arthritis and preparation method thereof |
| CN103478405B (en) * | 2013-06-13 | 2015-02-25 | 河南科技大学 | Method used for preparing probiotic preparations by using vitamin B2 fermentation liquid waste |
| CN107907608A (en) * | 2017-11-13 | 2018-04-13 | 济南维瑞医药科技开发有限公司 | Vitamin B in one kind detection multivitamin capsule2Method in relation to material |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB760072A (en) * | 1953-07-16 | 1956-10-31 | American Cyanamid Co | Improvements relating to the production of riboflavin |
| CN1504564A (en) * | 2002-12-05 | 2004-06-16 | Gj��ʽ���� | Microorganism for producing riboflavin and method for producing riboflavin using the same |
| CN1765897A (en) * | 2004-10-27 | 2006-05-03 | 上海凯赛生物技术研发中心有限公司 | Lactochrome fermentation liquor extraction process |
| CN101238220A (en) * | 2005-06-06 | 2008-08-06 | 帝斯曼知识产权资产管理有限公司 | Recycling of biomasses subjected to lysis as nutrient medium on fermentative production of riboflavin |
| CN101412716A (en) * | 2008-11-28 | 2009-04-22 | 山东恩贝生物工程有限公司 | Method for extracting lactoflavin |
-
2010
- 2010-12-28 CN CN2010106087532A patent/CN102086199B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB760072A (en) * | 1953-07-16 | 1956-10-31 | American Cyanamid Co | Improvements relating to the production of riboflavin |
| CN1504564A (en) * | 2002-12-05 | 2004-06-16 | Gj��ʽ���� | Microorganism for producing riboflavin and method for producing riboflavin using the same |
| CN1765897A (en) * | 2004-10-27 | 2006-05-03 | 上海凯赛生物技术研发中心有限公司 | Lactochrome fermentation liquor extraction process |
| CN101238220A (en) * | 2005-06-06 | 2008-08-06 | 帝斯曼知识产权资产管理有限公司 | Recycling of biomasses subjected to lysis as nutrient medium on fermentative production of riboflavin |
| CN101412716A (en) * | 2008-11-28 | 2009-04-22 | 山东恩贝生物工程有限公司 | Method for extracting lactoflavin |
Non-Patent Citations (2)
| Title |
|---|
| 仪宏等.核黄素生产技术进展.《中国食品添加剂》.2003,第4卷第14-18页. * |
| 章克昌等.从发酵液中分离提取核黄素.《无锡轻工大学学报》.1998,第17卷(第3期),第26-30页. * |
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| CN102086199A (en) | 2011-06-08 |
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