CN101608203A - A kind of fish skin collagen peptide and preparation technology and purposes with humidity-holding effect - Google Patents
A kind of fish skin collagen peptide and preparation technology and purposes with humidity-holding effect Download PDFInfo
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- CN101608203A CN101608203A CNA2008100480840A CN200810048084A CN101608203A CN 101608203 A CN101608203 A CN 101608203A CN A2008100480840 A CNA2008100480840 A CN A2008100480840A CN 200810048084 A CN200810048084 A CN 200810048084A CN 101608203 A CN101608203 A CN 101608203A
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- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 13
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- 238000003756 stirring Methods 0.000 claims description 9
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- 108090000145 Bacillolysin Proteins 0.000 claims description 7
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- 102000035092 Neutral proteases Human genes 0.000 claims description 7
- AXCZMVOFGPJBDE-UHFFFAOYSA-L calcium dihydroxide Chemical compound [OH-].[OH-].[Ca+2] AXCZMVOFGPJBDE-UHFFFAOYSA-L 0.000 claims description 7
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- 102000004169 proteins and genes Human genes 0.000 claims description 7
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- 108091005804 Peptidases Proteins 0.000 abstract description 3
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- YWIVKILSMZOHHF-QJZPQSOGSA-N sodium;(2s,3s,4s,5r,6r)-6-[(2s,3r,4r,5s,6r)-3-acetamido-2-[(2s,3s,4r,5r,6r)-6-[(2r,3r,4r,5s,6r)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2- Chemical compound [Na+].CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 YWIVKILSMZOHHF-QJZPQSOGSA-N 0.000 description 5
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- Peptides Or Proteins (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
The present invention has disclosed a kind of fish skin collagen peptide and preparation technology and purposes with humidity-holding effect, be exactly to utilize alkaline solution to handle fish-skin specifically, utilize the mode of protease hydrolysis to handle above-mentioned fish-skin again, fish skin collagen peptide that obtains by the technical finesse of hydrolyzed solution being carried out a series of decolourings, take off flavor, removing impurity and preparation technology thereof and as the purposes of makeup or skin-care product additive.Fish skin collagen peptide of the present invention is compared with general collagen peptide, small-molecular peptides content height not only, and also ash content is lower, the protein content height, also have on the market moisture-keeping efficacy the strongest in the like product,, vast potential for future development is arranged as makeup that have no side effect or skin-care product additive.
Description
Technical field
The present invention relates to a kind of fish skin collagen peptide and preparation technology and purposes with humidity-holding effect.Specifically, the present invention relates to a kind of alkaline solution that utilizes and handle fish-skin, utilize the mode of protease hydrolysis to handle above-mentioned fish-skin again, the fish skin collagen peptide that obtains by the technical finesse of hydrolyzed solution being carried out a series of decolourings, take off flavor, removing impurity, and preparation technology and as the purposes of makeup or skin-care product additive with humidity-holding effect.
Background technology
Collagen is a kind of scleroproein in the organism, mainly is present in the tissues such as skin, bone, cartilage and tendon, accounts for 25%～33% of human body or other animal body total protein contents.Collagen protein is widely used in biomedical materials field because of its good biological characteristics.Therefore, the extraction of collagen how to carry out the biologically active of high purity, high yield is the problem that numerous investigators pay close attention to always.The history of extracting collagen from pigskin and ox-hide is longer, and owing to its raw material abundance, remains the main source of collagen so far; Collagen mainly extracts from animal tissuess such as ox and pig for a long time.The people that make of mad cow disease and foot and mouth disease begin to consider to use the substitute of ox, pig collagen in recent years, and the extraction of fish skin collagen becomes the new focus of research in recent years.The minimum unit of collagen is that collagen tripeptide (Collagen tripeptide) is called for short CTP.Its structure can be expressed as (Gly-X-Y), is a kind of highly purified collagen tripeptide that has glycine at the N end.Trace ray auto photographing method studies show that CTP has the molecular-weight average of about 280Da.And most collagen peptide in the market mainly constitute polyamino acid by 30 to 100 amino acid. molecular-weight average reaches 3000-10000Da, and its bioavailability is limited.
Summary of the invention
The purpose of this invention is to provide a kind of is raw material with the fish-skin, is rich in the fish skin collagen peptide with moisture-keeping efficacy of small-molecular peptides (molecular weight 280Da).
Another object of the present invention provides described preparation technology with fish skin collagen peptide of moisture-keeping efficacy.
Another object of the present invention provides described purposes with fish skin collagen peptide of moisture-keeping efficacy.
Technical scheme of the present invention is: a kind of fish skin collagen peptide with moisture-keeping efficacy, form by following prepared, and described technology comprises the steps: successively
1) takes by weighing the fish-skin raw material, clean, drain away the water, weigh.
2) fish-skin of above-mentioned processing is added the water of 5～6 times of its quality, add the calcium hydroxide of its quality 3.0～5.0% again, stir and soaked 5～7 days, in the noncollagen protein in removing fish-skin, also played certain raw meat effect of going.
3) fish-skin of above-mentioned processing is cleaned to neutral, drained away the water, weigh, the gained fish-skin is the raw material after the pre-treatment.
4) with the fish-skin raw material after the above-mentioned processing according to its quality 5～6 water extraordinarily, 100 ℃ are boiled 60～90min, be cooled to 50～60 ℃, double gauze elimination residue, adjust pH is 6.0～8.0,50 ℃ of waters bath with thermostatic control, the bacillus alkaline protease that at first adds fish-skin raw materials quality 0.5～1.0%, add the bromeline of fish-skin raw materials quality 0.5～1.0% and the neutral protease of fish-skin raw materials quality 0.5～1.0% more simultaneously, pick up counting from adding bacillus alkaline protease, enzymolysis is 5～6 hours altogether.After enzymolysis finishes, more than 95 ℃, 10 minutes enzymes that go out, cooling, filter cloth filters, and filtrate is the fish skin collagen peptide hydrolysate.
5) above-mentioned filtrate is taken off flavor with the K15 activity carbon column decoloring.
6) filtrate of above-mentioned decolouring being taken off behind the flavor is concentrated into suitable volume, and spraying drying makes finished product.
Another technical scheme of the present invention is: a kind of preparation has the technology of the fish skin collagen peptide of moisture-keeping efficacy, it is characterized in that: comprise the steps: successively
1) takes by weighing the fish-skin raw material, clean, drain away the water, weigh.
2) fish-skin of above-mentioned processing is added the water of 5～6 times of its quality, add the calcium hydroxide of its quality 3.0～5.0% again, stir and soaked 5～7 days, in the noncollagen protein in removing fish-skin, also played certain raw meat effect of going.
3) fish-skin of above-mentioned processing is cleaned to neutral, drained away the water, weigh, the gained fish-skin is the raw material after the pre-treatment.
4) with the fish-skin raw material after the above-mentioned processing according to its quality 5～6 water extraordinarily, 100 ℃ are boiled 60～90min, be cooled to 50～60 ℃, double gauze elimination residue, adjust pH is 6.0～8.0,50 ℃ of waters bath with thermostatic control, the bacillus alkaline protease that at first adds fish-skin raw materials quality 0.5～1.0%, add the bromeline of fish-skin raw materials quality 0.5～1.0% and the neutral protease of fish-skin raw materials quality 0.5～1.0% more simultaneously, pick up counting from adding bacillus alkaline protease, enzymolysis is 5～6 hours altogether.After enzymolysis finishes, more than 95 ℃, 10 minutes enzymes that go out, cooling, filter cloth filters, and filtrate is the fish skin collagen peptide hydrolysate.
5) above-mentioned filtrate is taken off flavor with the K15 activity carbon column decoloring.
6) filtrate of above-mentioned decolouring being taken off behind the flavor is concentrated into suitable volume, and spraying drying makes finished product.
Another technical scheme of the present invention is: the described purposes with fish skin collagen peptide of moisture-keeping efficacy as makeup or skin-care product additive of a kind of claim 1.
Fish skin collagen peptide of the present invention, improve small-molecular peptides in the fish skin collagen peptide (about molecular weight 280Da) components contents to greatest extent, experimental results show that: because the CTP molecular weight is very little, thereby can effectively infiltrate stratum corneum, in skin corium and the hair root cell, can promote that the synthetic and skin corium of collagen is fibrocellular hyaluronic synthetic.The CTP component is made an addition in the daily necessities of skin cleaning liquid class, will be to the elasticity of skin, wettability presents very excellent effect with the coarse state that improves skin, and have safe, the cell agglutinating value(of coal) is good, transparent, odorless and characteristics such as have no stimulation, can be used as the additive of makeup or skin care product, vast market prospect is arranged.
Fish skin collagen peptide by preparation technology's production of the present invention with humidity-holding effect; have good cell agglutinating value(of coal), absorptivity and Bc; in makeup as wetting Agent for Printing Inks and Derma-Guard; can promote epithelial cell to form; directly replenish the collagen protein that human body runs off, delaying decrepitude of skin can be used for anti-wrinkle, preserves moisture and reparation etc.; and when being applied to skin surface, presenting excellent humidity-holding effect and phenomenon hypersensitive can not occur at all.
Description of drawings
Accompanying drawing 1 has the process flow diagram of the fish skin collagen peptide of moisture-keeping efficacy for the present invention's preparation.
Embodiment
Understand the present invention's preparation below by embodiment and have the technology of the fish skin collagen peptide of moisture-keeping efficacy, but following embodiment does not limit content of the present invention.
The present invention's preparation has the technology of the fish skin collagen peptide of moisture-keeping efficacy
1) raw material pre-treatment.Clean fish-skin, control water (weighing), add the water of 5～6 times of its quality, the calcium hydroxide that adds its quality 3.0～5.0% again stirs and soaked 5～7 days, with the fish-skin after the abundant cleaning and dipping of tap water, till about pH value to 7.0, control water (weighing) is standby.Through alkaline solution treatment process of the present invention, when removing the noncollagen protein in the raw material, also played certain raw meat effect of going.
2) sex change.According to 5～6 distilled water extraordinarily of above-mentioned wet basis raw materials quality, 100 ℃ are boiled sex change 60～90min.
3) filter.Hydrolyzed solution after the above-mentioned sex change is cooled to 50～60 ℃, and with double gauze elimination fish scale residue and grease, visual grease removal situation repeats to filter several times.Moisture evaporated when adding distil water is supplied sex change.
4) protease hydrolysis.Adjust pH to 6.0～8.0, constant temperature is at 50 ℃, the bacillus alkaline protease that in above-mentioned filtrate, adds 1 described wet basis raw materials quality 0.5～1.0%, add the bromeline of 1 described wet basis raw materials quality 0.5～1.0% and the neutral protease of 1 described wet basis raw materials quality 0.5～1.0% more simultaneously, pick up counting from adding bacillus alkaline protease, stirred enzymolysis altogether 5～6 hours (during moisturizing in good time, keep the material liquid mixture constancy of volume).
5) deactivation.With said hydrolyzed liquid more than 95 ℃, deactivation 10min.
6) filter.Hydrolyzed solution after the above-mentioned deactivation is cooled to 50～60 ℃, and filter cloth filters.
7) the active carbon column adsorption bleaching takes off flavor.Above-mentioned filtrate is taken off flavor with the K15 activity carbon column decoloring, note liquid running balance in the control post, in order to avoid leak carbon.
8) drying.Above-mentioned filtrate is concentrated into suitable volume, and spraying drying makes finished product.
The embodiment 1 that has the fish skin collagen peptide of moisture-keeping efficacy according to above-mentioned prepared:
1, the fish-skin that thaws freezes piece 10kg, clean residual meat and bloodstain, drop in weigh about 9kg fish-skin, add 54L nature water and 270g calcium hydroxide, mixing soaks 5 days (stirring every day 2 times), after 5 days, with nature water (200L, 2h consuming time) be washed till neutrality, draining weighs must refrigerate standby by 6 kilograms of fish-skins (fish scale of washing off discards) approximately.
2, platform balance takes by weighing the standby fish-skin of 1kg refrigeration, presses 1: 5 adding distil water 5L of solid-liquid ratio, and 100 ℃ are boiled sex change 60min.
3, double gauze filters and removes the fish-skin residue, 50 ℃ of waters bath with thermostatic control, and transferring pH value is 7.0, add the 8g bacillus alkaline protease, add 8g bromeline and 8g neutral protease again, pick up counting, stir enzymolysis 5h altogether automatically from adding bacillus alkaline protease.
4, water-bath deactivation (95 ℃ 10min), are cooled to 50 ℃～60 ℃.
5,300 order filter clothes filter, and get the about 4700ml of filtrate volume.
6, active carbon column (K15 granulated carbon, blade diameter length ratio 1: 4) takes off flavor, adds 1800ml distillation washing carbon post, is eluted to total liquid volume 6500ml
7, filtrate spraying drying gets the fish skin collagen peptide that 110 grams have moisture-keeping efficacy.
The embodiment 2 that has the collagen peptide of moisture-keeping efficacy according to above-mentioned prepared:
1, the fish-skin that thaws freezes piece 9kg, clean residual meat and bloodstain, drain weigh about 8kg fish-skin, add 40L nature water and 240g calcium hydroxide, mixing soaks 7 days (stirring every day 2 times), after 7 days, with nature water (200L, 2h consuming time) be washed till neutrality, draining weighs must refrigerate standby by 5.5 kilograms of fish-skins (fish scale of washing off discards) approximately.
2, platform balance takes by weighing the standby fish-skin of 1kg refrigeration, presses 1: 5.5 adding distil water 5.5L of solid-liquid ratio, and 100 ℃ are boiled sex change 80min.
3, double gauze filters and removes the fish-skin residue, 50 ℃ of waters bath with thermostatic control, and transferring pH value is 8.0, add the 9g bacillus alkaline protease, add 9g bromeline and 9g neutral protease again, pick up counting, stir enzymolysis 5.5h altogether automatically from adding bacillus alkaline protease.
4, water-bath deactivation (95 ℃ 10min), are cooled to 50 ℃～60 ℃.
5,300 order filter clothes filter, and get the about 4900ml of filtrate volume.
6, active carbon column (K15 granulated carbon, blade diameter length ratio 1: 4) takes off flavor, adds 2000ml distillation washing carbon post, is eluted to total liquid volume 6900ml
7, filtrate spraying drying gets the fish skin collagen peptide that 120 grams have moisture-keeping efficacy.
Product index by the fish skin collagen peptide with moisture-keeping efficacy of prepared in this embodiment is:
Total nitrogen 〉=15.5%
Moisture≤5.0% ash content≤2%
Fat≤0.5% oxyproline accounts for protein content (%) 8.5～10.0
Polypeptide 〉=90% molecular-weight average, 90%≤1000D
Its component 90% left and right sides molecular weight is below 1000Da, and wherein small-molecular peptides (molecular weight 280Da) content is about 50%, and its physical and chemical index is as shown in table 1:
Table 1 fish skin collagen peptide physical and chemical index table
Moisture-keeping efficacy research work of the present invention is summarized as follows:
1 body surface instrumental measurement
1.1 test principle
Adopt SK-III numeral moisture content tester (Kai Er of Shenzhen Electronics Factory) to measure the variation of skin surface water ratio.Represent the moisture retention of collagen peptide with water ratio, water ratio is high more, illustrates that the skin surface moisture content is high more, collagen peptide to improve moisture of skin function (performance of keeping humidity) good more.
1.2 experiment material
1.2.1 tried thing
Fish skin collagen peptide: be called for short fish, self-control;
Other samples: the collagen product of the same type on the market, amount to 6 kinds, count sample 1-sample 6.
1.2.2 reagent
Cosmetics-stage hyaluronate sodium (positive control): east, Shandong occasion bio-engineering corporation provides.
1.2.3 instrument
SK-III numeral moisture content tester (Kai Er of Shenzhen Electronics Factory); Stopwatch; Cotton swab.
1.2.4 environment
Room temperature is below 25 ℃, and relative humidity is below 70%
1.3 experimental technique
Blank group, same concentrations with the distilled water that does not add collagen peptide are tried thing solution, and 0.3% sodium hyaluronate solution (positive control) is measured at people's arm inside line, and measuring method is as follows:
1. the arm inside line in the tester selects the quilt of proper area to test the district, is tested at quilt and distinguishes tick (being tested the quantity of district's quantity by sample decides).
2. measure the top layer moisture content (t=0) at each position respectively.
3. the solution of materialsing glues with cotton swab gets even coating, (blank with the distilled water coating, positive control applies with 0.3% hyaluronate sodium).Wipe drop with filter paper after 60 seconds.
4. open SK-III numeral moisture content tester after the sample drop is wiped immediately and begin to measure the water ratio of being tested the district, constantly zero clearing redeterminated the water ratio of being tested the district about 120 seconds then.
5. with water ratio (%) and time (to calculate second, be abbreviated as s) be that coordinate is mapped, same tester, determination of moisture value during with blank t=0 is a standard, all the other respectively tried thing (comprising positive control) again the difference of the determination of moisture value when the determination of moisture value when respectively being tried thing t=0 and blank t=0 the determination of moisture value of each time point is converted, make that respectively to be tried the thing initial value identical, respectively to be tried the power that thing improves the moisture of skin function.
1.4 test crowd
Amount to 12 people, age bracket 20～50 years old, each 6 people of men and women.
1.5 precaution
1.5.1 spacious, ventilation condition is good, the inspection chamber that space environments such as temperature, humidity are stable carries out.
1.5.2 allowed experimenter under the regulation environment tranquil 10 minutes before on-test.
1.5.3 samely tested district's mensuration work and undertaken by same instrument, same people.
1.6 result and analysis
1.6.1 the comparison of like product body surface humidity-holding effect on fish skin collagen peptide and the market
Tried thing solution and tested by solid-liquid ratio 1: 4 preparation, be the results are shown in Table 2 according to 1.3 method:
Table 2 respectively tried object table moisture determination data sheet (X ± s, n=12)
By table 2 as seen, the humidity-holding effect of fish skin collagen peptide is best in the like product on market, and its small-molecular peptides that is rich in (molecular weight 280Da) can keep moisture of skin effectively.With regard to precision, the barren variation coefficient (CV) is: 2.21%～4.33%, the variation coefficient of positive control (CV) is: 0.82%～4.08%, the variation coefficient (CV) of respectively being tried thing is: 0.76%～4.27%, above normal condition variation (RCV) and top condition variation (OCV) all common requirement 15% and 7% in.
2 in vitro tests methods
2.1 test principle
Ammonium sulfate [(NH
4)
2SO
4] saturated solution relative humidity about 80%.Through long-time test, the ambient relative humidity that fills ammonium sulfate saturated solution moisture eliminator is 85%.
According to the difference of wetting Agent for Printing Inks performance of keeping humidity, different wetting Agent for Printing Inks molecules are to the reactive force difference of water molecules, and it is also different with the ability that keeps moisture to absorb moisture.Reactive force is big, and is strong to water molecules power, and the amount of absorption and maintenance water is also bigger.The sample that contains certain moisture is placed in the moisture eliminator of fixed temperature and humidity dry, and regularly the minimizing of weighing sample quality by comparative analysis, compares the size of different sample moisture retentions.
For the better practical situation of simulated skin, stick the 3M adhesive plaster to slide glass, the aqueous solution of wetting Agent for Printing Inks is applied on it, weigh after putting the moisture eliminator certain hour into, calculate percentage of water loss, the low more explanation humidity-holding effect of percentage of water loss is good more.
2.2 experiment material
2.2.1 tried thing consubstantiality watch test
2.2.2 reagent
Cosmetics-stage hyaluronate sodium (positive control): east, Shandong occasion bio-engineering corporation;
Ammonium sulfate (constant humidity agent): Beijing chemical reagents corporation of traditional Chinese medicines group.
2.2.3 instrument
Moisture eliminator; Wetbulb thermometer; Electronic balance; Slide glass; The 3M adhesive plaster.
2.3 experimental technique
2.3.1 testing sequence
With an amount of 0.3% sodium hyaluronate solution (positive control), tried thing solution, distilled water (blank) is coated on the slide glass that posts the 3M adhesive plaster, put into the moisture eliminator that fills saturated ammonium sulphate solution (relative humidity is 85%), carry out weighing every 1～2 hour (h), calculate its percentage of water loss in this time period.
2.3.2 method of calculation
Percentage of water loss=(m
0-m
t)/m
0* 100%
M in the formula
tQuality for moisture behind the placement th; m
0Moisture quality for beginning.
2.4 result and analysis
2.4.1 the comparison of the external humidity-holding effect of like product on fish skin collagen peptide and the market
To respectively be tried thing by 1: 4 wiring solution-forming of solid-liquid ratio, be tested, be the results are shown in Table 3 according to 2.3.1:
Table 3 respectively tried object test outward the humidity-holding effect comparison sheet (X ± s, n=6)
Annotate:
*The variation coefficient (CV) is greater than 7% of the OCV regulation, but less than 15% of RCV regulation, all the other all satisfy the OCV requirement.
The dehydration summation of the percentage of water loss of unit interval and some hrs as can be seen from Table 3, fish skin collagen peptide is all less, last several hrs since other loss of water of being tried thing totally, so the percentage of water loss of unit time is little than fish skin collagen peptide, this is also positive and negative foil fish skin collagen peptide can be more lasting and stably keep moisture.With regard to precision, the barren variation coefficient (CV) is: 1.01%～13.29%, the variation coefficient of positive control (CV) is 0.19%～2.40%, the variation coefficient (CV) of respectively being tried thing is: 0.53%～13.49%, all the other all meet the requirement of normal condition variation (RCV) all less than 7% 7% except that two places surpass.
By above analysis as can be known, use the fish skin collagen peptide with moisture-keeping efficacy of prepared in this embodiment, except that nutritive ingredient with general collagen product, also have on the market moisture-keeping efficacy the strongest in the like product, can be used as the makeup or the skin-care product additive that have no side effect, have extensive market prospects.
Claims (10)
1. the fish skin collagen peptide with moisture-keeping efficacy is formed by following prepared, and described technology comprises the steps: successively
1) takes by weighing the fish-skin raw material, clean, drain away the water, weigh.
2) fish-skin of above-mentioned processing is added the water of 5～6 times of its quality, add the calcium hydroxide of its quality 3.0～5.0% again, stir and soaked 5～7 days, in the noncollagen protein in removing fish-skin, also played certain raw meat effect of going.
3) fish-skin of above-mentioned processing is cleaned to neutral, drained away the water, weigh, the gained fish-skin is the raw material after the pre-treatment.
4) with the fish-skin raw material after the above-mentioned processing according to its quality 5～6 water extraordinarily, 100 ℃ are boiled 60～90min, be cooled to 50～60 ℃, double gauze elimination residue, adjust pH is 6.0～8.0,50 ℃ of waters bath with thermostatic control, the bacillus alkaline protease that at first adds fish-skin raw materials quality 0.5～1.0%, add the bromeline of fish-skin raw materials quality 0.5～1.0% and the neutral protease of fish-skin raw materials quality 0.5～1.0% more simultaneously, pick up counting from adding bacillus alkaline protease, enzymolysis is 5～6 hours altogether.After enzymolysis finishes, more than 95 ℃, 10 minutes enzymes that go out, cooling, filter cloth filters, and filtrate is the fish skin collagen peptide hydrolysate.
5) above-mentioned filtrate is taken off flavor with the K15 activity carbon column decoloring.
6) filtrate of above-mentioned decolouring being taken off behind the flavor is concentrated into suitable volume, and spraying drying makes finished product.
2. by the described fish skin collagen peptide of claim 1, it is characterized in that: the molecular weight of the component 90% of described fish skin collagen peptide is below 1000Da, and wherein the small-molecular peptides content of molecular weight 280Da is 50%.
3. by the described fish skin collagen peptide of claim 1, it is characterized in that: the product index of described fish skin collagen peptide is:
Total nitrogen 〉=15.5%
Moisture≤5.0% ash content≤2%
Fat≤0.5% oxyproline accounts for protein content (%) 8.5～10.0
Polypeptide 〉=90% molecular-weight average 90%≤≤ 1000D
4. by the described fish skin collagen peptide of claim 1, it is characterized in that: the molecular weight distribution of described fish skin collagen peptide sees the following form:
5. by the described fish skin collagen peptide of claim 1, it is characterized in that: described fish skin collagen peptide physical and chemical index sees the following form:
6. a technology for preparing the fish skin collagen peptide with moisture-keeping efficacy is characterized in that: comprise the steps: successively
1) takes by weighing the fish-skin raw material, clean, drain away the water, weigh.
2) fish-skin of above-mentioned processing is added the water of 5～6 times of its quality, add the calcium hydroxide of its quality 3.0～5.0% again, stir and soaked 5～7 days, in the noncollagen protein in removing fish-skin, also played certain raw meat effect of going.
3) fish-skin of above-mentioned processing is cleaned to neutral, drained away the water, weigh, the gained fish-skin is the raw material after the pre-treatment.
4) with the fish-skin raw material after the above-mentioned processing according to its quality 5～6 water extraordinarily, 100 ℃ are boiled 60～90min, be cooled to 50～60 ℃, double gauze elimination residue, adjust pH is 6.0～8.0,50 ℃ of waters bath with thermostatic control, the bacillus alkaline protease that at first adds fish-skin raw materials quality 0.5～1.0%, add the bromeline of fish-skin raw materials quality 0.5～1.0% and the neutral protease of fish-skin raw materials quality 0.5～1.0% more simultaneously, pick up counting from adding bacillus alkaline protease, enzymolysis is 5～6 hours altogether.After enzymolysis finishes, more than 95 ℃, 10 minutes enzymes that go out, cooling, filter cloth filters, and filtrate is the fish skin collagen peptide hydrolysate.
5) above-mentioned filtrate is taken off flavor with the K15 activity carbon column decoloring.
6) filtrate of above-mentioned decolouring being taken off behind the flavor is concentrated into suitable volume, and spraying drying makes finished product.
7. by the preparation technology of the described fish skin collagen peptide of claim 6, it is characterized in that: the product index of prepared fish skin collagen peptide is:
Total nitrogen 〉=15.5%
Moisture≤5.0% ash content≤2%
Fat≤0.5% oxyproline accounts for protein content (%) 8.5～10.0
Polypeptide 〉=90% molecular-weight average, 90%≤1000D
8. by the preparation technology of the described fish skin collagen peptide of claim 6, it is characterized in that: the molecular weight distribution of prepared fish skin collagen peptide sees the following form:
9. by the preparation technology of the described fish skin collagen peptide of claim 6, it is characterized in that: prepared fish skin collagen peptide physical and chemical index sees the following form:
10. described purposes of claim 1 with fish skin collagen peptide of moisture-keeping efficacy as makeup or skin-care product additive.
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