CN101366449B - Microorganism vitamin premixed material and preparation method - Google Patents
Microorganism vitamin premixed material and preparation method Download PDFInfo
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- CN101366449B CN101366449B CN2007101204078A CN200710120407A CN101366449B CN 101366449 B CN101366449 B CN 101366449B CN 2007101204078 A CN2007101204078 A CN 2007101204078A CN 200710120407 A CN200710120407 A CN 200710120407A CN 101366449 B CN101366449 B CN 101366449B
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Abstract
The present invention relates to a microbe vitamin premix material and a method for preparing the same, in particular to a microbe vitamin premix material consisting of a plurality of useful microbes and vitamins and a method for preparing the same. The microbe vitamin premix material comprises bacillus, microzyme, enterococcus, vitamin A, vitamin D3, vitamin E, vitamin K3, vitamin B1, vitamin B2, vitamin B6 and vitamin B12, and is the microbe vitamin premix material generally for pigs, poultries, ruminant or aquatic animals. The microbe vitamin premix material has the animal immunization health care function as well as nutrient efficacy, and can improve illness resistant capability of animals, reduce the consumption of antibacterial drugs in feed, lower the drug resistant degree of bacteria, improve the feed utilization ratio, promote health of mankind and improve the ecological environment.
Description
Technical field
The present invention relates to the microorganism application, be specifically related to a kind of microorganism vitamin premixed material and preparation method thereof.
Background technology
Preventative and the interpolation growth promotion medicine has promoted the development of China's aquaculture greatly in feed and the aquaculture, and domestic premix product more than 95% all adds growth promotion and preventative antibacterials at present.In premix, add antibacterials and blindly abusing antibacterials has caused a series of problem in breeding process: the one, feed and aquaculture are to the serious dependence of antibacterials, make in the animal body and breeding environment in the drug resistance aggravation of pathogenetic bacteria and virus, thereby showing drug effect reduces, aquaculture cost rises, caused in the animal body and the destruction of external ecological environment, strengthened animal epidemic prevention and control difficulty; The 2nd, cause livestock product Chinese traditional medicine residue problem serious, product quality and security descend, and the residual problem of medicine makes China's livestock product outlet be obstructed again and again, and the economic loss heaviness has reduced the market competitiveness and consumer's degree of belief; The 3rd, cause environmental pollution serious, there are a large amount of drug-resistant bacterias and harmful farm animal excrement in the breeding environment, make the deterioration of the ecological environment to threaten human health etc.As far back as 1999, European Union just issued and forbidden using antibiotic decree in feed, and began to completely forbid in 2006 add antibiotic in feed.Reduce or forbid adding antibacterials in feed, the use of antibacterials is by the countries in the world common concern in the standard breeding process.
Additive for microbe feedstuff is to be used for reducing and substituting one of first-selected product of feeding antibacterials.But this kind product uses as a kind of additive agent for feeding separately in the animal-breeding process mostly, does not add in feed formula, therefore, can not effectively reach the purpose of adding feeding antibiotic in minimizing or the alternative feed.
Summary of the invention
Purpose of the present invention aims to provide the microorganism vitamin feed pre-mixing material that a kind of existing animal immune health care possesses nutritive effect again, thereby improve the animal disease resistant ability, reduce the use of antibacterials in the feed, alleviate the bacterial resistance degree, improve efficiency of feed utilization, promote human health, improve the ecological environment.
Another object of the present invention provides the preparation method of described microorganism vitamin premixed material.
The objective of the invention is to be achieved through the following technical solutions:
A kind of microorganism vitamin premixed material is characterized in that comprising bacillus, saccharomycete and enterococcus bacterium powder, vitamin and carrier.Wherein, described bacillus is one or both in bacillus subtilis, the bacillus licheniformis; Described saccharomycete is one or both in saccharomyces cerevisiae, the candida utili; Described enterococcus is one or more in enterococcus faecalis, VREF, the lactoenterococcus.
Bacillus of the present invention, saccharomycete and enterococcus bacterium powder are solid bacterium powder, have adopted following preparation method:
(1) bacillus bacterium powder prepares as follows: the bacillus bacterial classification is cultivated through the deep liquid aerobic fementation, seed culture 8 hours, inoculum concentration 1%, 36～37 ℃ of cultivation temperature.Speed of agitator 280～300rpm, tank pressure 0.06MPa, air mass flow 1: 0.4～0.6, the dissolved oxygen relative value is controlled at 80%～90%, and fermentation time 16～24h, zymotic fluid directly carry out spray-drying and make.Wherein seed culture medium and fermentation medium are respectively in following ratio liquid mixture prepared (g/L): seed culture medium: wheat bran 50, fish meal 3.0～4.0, dregs of beans 10.0, yeast extract 3.0～4.0, NaOH 2.0, water boil 1h is neutralized to 6.8～7.0 with hydrochloric acid; Fermentation medium: wheat bran 50, dregs of beans 20, sodium carbonate 0.3～0.4, defoamer 0.4～0.7.Sterilized 30 minutes for 121 ℃;
(2) saccharomycete bacterium powder prepares as follows: saccharomycete bacterial classification liquid deep layer fermenting is cultivated, seed culture 8 hours, inoculum concentration 3～6%, 28～30 ℃ of cultivation temperature.Speed of agitator 150～250rpm, tank pressure 0.06MPa, air mass flow 1: 0.4～0.6, the dissolved oxygen relative value is controlled at 80%～90%, fermentation time 24～50h, and zymotic fluid adds carrier, described carrier is zein and soluble starch, its weight proportion is: zein 5～95%, and soluble starch 5～95%, the ratio of carrier and bacterium liquid is 1: 0.3～0.6.Mixing 30～40 ℃ of fluidized dryings in back makes.Wherein seed culture medium and fermentation medium are respectively in following ratio liquid mixture prepared (g/L): seed culture medium: molasses 70～80, peptone 5～10, ammonium sulfate 1.0, epsom salt 0.5; Fermentation medium: molasses 70～80, urea 2～10, sodium chloride 1.0.Sterilized 30 minutes for 121 ℃.
(3) enterococcus bacterium powder prepares as follows: enterococcus liquid fermentation and culture, seed culture 8 hours, inoculum concentration 3～6%, 30～35 ℃ of cultivation temperature.Fermentation time 24～50h, zymotic fluid adds carrier, and described carrier is zein and soluble starch, and its weight proportion is: zein 5～95%, soluble starch 5～95%, the weight ratio of carrier and bacterium liquid is 1: 0.3～0.6.Mixing 30～40 ℃ of fluidized dryings in back makes.Wherein seed culture medium and fermentation medium are respectively in following ratio liquid mixture prepared (g/L): seed culture medium: glucose 10～30, yeast extract 5～15, dipotassium hydrogen phosphate 2.0～5.0; Potassium dihydrogen phosphate 2.0～5.0; Fermentation medium: glucose: 10～30, yeast extract 2.0～10, sodium chloride 1.0.Sterilized 30 minutes for 121 ℃.
Then, cultivate used culture medium and the condition of culture of rejuvenation according to separately flat board respectively, the viable bacteria content of above-mentioned (1), (2), (3) solid powder is carried out conventional plate count detect,, utilize carrier that viable bacteria is diluted to 2 * 10 respectively according to count results
10Cfu/g, described vehicle weight proportioning is: zein 5%～95%, soluble starch 5%～95%.
Vitamin of the present invention comprises vitamin A, vitamin D
3, vitamin E, vitamin K
3, Cobastab
1, Cobastab
2, Cobastab
6And Cobastab
12
Described carrier be in glucose, lactose, sucrose, soluble starch, wheat bran, defatted rice bran, powdered rice hulls, maize cob meal, corn protein powder, the stone flour choose any one kind of them or multiple.
Bacillus, saccharomycete and enterococcus bacterium grain weight amount (wt) proportioning are 1.5～60% in the microorganism vitamin premixed material of the present invention, and vitamin is 0.58～6.64%; In particular: bacillus is 0.5～20%; Saccharomycete 0.5～20%; Enterococcus 0.5～20%; Vitamin A 0.02～0.85%; Vitamin D
30.01～0.25%; Vitamin E 0.5～3.6%; Vitamin K
30.01～0.8%; Cobastab
10.01～0.35%; Cobastab
20.01～0.44%; Cobastab
60.01～0.26%; Cobastab
120.01～0.09%.
Microorganism vitamin premixed material provided by the invention is meant that pig uses universal microorganism vitamin premixed material with universal microorganism vitamin premixed material, ruminant with universal microorganism vitamin premixed material or aquatic livestock with universal microorganism vitamin premixed material, fowl.
Prepare according to following weight ratio respectively according to different practical uses:
Pig is as follows with the weight proportion of universal microorganism vitamin premixed material: bacillus 2.5～20%; Saccharomycete 2.5～20%; Enterococcus 2.5～20%; Vitamin A 0.2～0.7%; Vitamin D
30.03～0.1%; Vitamin E 0.8～2.7%; Vitamin K
30.04～0.11%; Cobastab
10.01～0.06%; Cobastab
20.16～0.34%; Cobastab
60.01～0.15%; Cobastab
120.01～0.08%; Carrier 38.6～91.24%.This pig is 1.5 * 10 with the viable count in the universal microorganism vitamin premixed material
9～12 * 10
9Cfu/g.
Fowl is as follows with the weight proportion of universal microorganism vitamin premixed material: bacillus 0.5～20%; Saccharomycete 5～10%; Enterococcus 0.5～20%; Vitamin A 0.35～0.51%; Vitamin D
30.1～0.14%; Vitamin E 0.7～1.02%; Vitamin K
30.1～0.2%; Cobastab
10.03～0.06%; Cobastab
20.12～0.22%; Cobastab
60.02～0.13%; Cobastab
120.03～0.08%; Carrier 47.65～92.1%.This fowl is 1.2 * 10 with the viable count in the universal microorganism vitamin premixed material
9～10
10Cfu/g.
Ruminant is as follows with the weight proportion of universal microorganism vitamin premixed material: bacillus 2.5～5%; Saccharomycete 5～20%; Enterococcus 2.5～5%; Vitamin A 0.14～0.85%; Vitamin D
30.01～0.25%; Vitamin E 0.5～2%; Vitamin K
30.01～0.4%; Cobastab
10.01～0.2%; Cobastab
20.01～0.36%; Cobastab
60.01～0.2%; Cobastab
120.01～0.09%; Carrier 65.65～89.30%.This ruminant is 2 * 10 with the viable count in the universal microorganism vitamin premixed material
9～6 * 10
9Cfu/g.
Aquatic livestock is as follows with the weight proportion of universal microorganism vitamin premixed material: bacillus 10～20%; Saccharomycete 10～15%; Enterococcus 10～20%; Vitamin A 0.18～0.32%; Vitamin D
30.02～0.1%; Vitamin E 1.2～3.6%; Vitamin K
30.08～0.8%; Cobastab
10.01～0.35%; Cobastab
20.06～0.44%; Cobastab
60.01～0.26%; Cobastab
120.01～0.06%; Carrier 39.07～68.43%.This aquatic livestock is 6 * 10 with the viable count in the universal microorganism vitamin premixed material
9～11 * 10
9Cfu/g.
The present invention also provides the preparation method of this microorganism vitamin premixed material, may further comprise the steps:
(1) preparation additive for microbe feedstuff bacterium powder;
(2) take by weighing selected raw material, and, a part of carrier and the vitamin gross weight that is taken by weighing are equated the carrier separated into two parts that takes by weighing;
(3) vitamin and the heavy carrier such as grade that takes by weighing mixed;
(4) another part carrier mixing that takes by weighing in bacterium powder that again step (1) is prepared and the step (2).
Wherein, in the step (3) vitamin and the heavy carrier such as grade that takes by weighing mixed, the present invention preferably puts into the mixing plant container and mixes, and more preferably mixes 2～30 minutes; Another part carrier mixing that takes by weighing in bacterium powder that in the step (4) step (1) is prepared and the step (2), the present invention preferably puts into the mixing plant container, mixes 5～50 minutes.
Wherein, mixing mixing plant of the present invention can be choosing any one kind of them in the efficient horizontal mixer of twin shaft, " V " type mixer, horizontal paddle mixer, the three-dimensional motion mixer.
Among the present invention additive for microbe feedstuff combined with vitamin and be developed to microorganism vitamin feed pre-mixing material product, have the digestion of the feed of promotion, regulate the animal microecological balance, the health care enteron aisle; Improve the animal nonspecific immunity, reduce antibacterials and use, alleviate the bacterial resistance degree; Improve in the body, external breeding environment and function such as reduce that disease is brought out; With nutritive effects such as replenishing vitamins.The present invention has not only solved the core technology that additive for microbe feedstuff is applied, and simultaneously also makes product function more comprehensively, uses more conveniently, and market plane is wider, and product has more competitiveness and industrialization prospect.
The above-mentioned subject area of the present invention not only is confined to following examples, and all technology that realizes based on foregoing of the present invention all belong to category of the present invention.
The specific embodiment
Embodiment 1: the preparation of bacillus
Seed culture medium is in following ratio liquid mixture prepared (g/L): wheat bran 50, and fish meal 3.0, dregs of beans 10, yeast extract 3.0, NaOH 2.0, water boil 1h is neutralized to 6.8～7.0 with hydrochloric acid.
Fermentation medium is in following ratio liquid mixture prepared (g/L): wheat bran 50, dregs of beans 20, sodium carbonate 0.3, defoamer 0.4.Sterilized 30 minutes for 121 ℃.
The deep liquid aerobic fementation is cultivated, seed culture 8 hours, inoculum concentration 1%, 36～37 ℃ of cultivation temperature.Speed of agitator 280～300rpm, tank pressure 0.06MPa, air mass flow 1: 0.4～0.6, the dissolved oxygen relative value is controlled at 80%～90%, and fermentation time 16h detects the gemma number later on, when having observed a large amount of gemma and producing, put jar, zymotic fluid directly carries out spray-drying and makes bacillus bacterium powder.Cultivate used culture medium and the condition of culture of rejuvenation according to flat board, the viable bacteria content of bacterium powder is carried out conventional plate count detect,, utilize carrier that viable bacteria is diluted to 2 * 10 respectively according to count results
10Cfu/g, the vehicle weight proportioning is: zein 95%, soluble starch 5%.
Embodiment 2: saccharomycetic preparation
Seed culture medium is in following ratio liquid mixture prepared (g/L): molasses 70～80, peptone 5～10, ammonium sulfate 1.0, epsom salt 0.5; PH is nature pH, sterilizes 30 minutes for 121 ℃.
Fermentation medium is in following ratio liquid mixture prepared (g/L): molasses 70～80, urea 2～10, sodium chloride 1.0; PH is nature pH, sterilizes 30 minutes for 121 ℃.
Liquid deep layer fermenting is cultivated, seed culture 12 hours, inoculum concentration 3～6%, 28～30 ℃ of cultivation temperature.Speed of agitator 150～250rpm, tank pressure 0.06MPa, air mass flow 1: 0.4～0.6, the dissolved oxygen relative value is controlled at 80%～90%, and fermentation time 24h detects the qualified jar of promptly putting of clump count later on.
The zymotic fluid cryogenic vacuum is concentrated into 40% of original volume, adds carrier in concentrate, and described carrier is zein and soluble starch, and its weight proportion is: zein 10%, the weight ratio of soluble starch 90% carrier and bacterium liquid is 1: 0.4.Mix 30～40 ℃ of fluidized dryings in back and make saccharomycete bacterium powder.
Cultivate used culture medium and the condition of culture of rejuvenation according to flat board, the viable bacteria content of bacterium powder is carried out conventional plate count detect,, utilize carrier that viable bacteria is diluted to 2 * 10 respectively according to count results
10Cfu/g, the vehicle weight proportioning is: zein 50%, soluble starch 50%.
Embodiment 3: enterococcal preparation
Seed culture medium is in following ratio liquid mixture prepared (g/L): glucose 10～30, yeast extract 5～15, dipotassium hydrogen phosphate 2.0～5.0; Potassium dihydrogen phosphate 2.0～5.0; PH is nature pH, sterilizes 30 minutes for 121 ℃.
Fermentation medium is in following ratio liquid mixture prepared (g/L): glucose: 10～30, and yeast extract 2.0～10, sodium chloride 1.0.PH is nature pH, sterilizes 30 minutes for 121 ℃.
Fermented and cultured, seed culture 12 hours, inoculum concentration 3～6%, cultivation temperature .30～35 ℃.Fermentation time 24～50h, fermentation time 24h detect the qualified jar of promptly putting of clump count later on.
The zymotic fluid cryogenic vacuum is concentrated into 30%～60% of original volume, adds carrier in concentrate, and described carrier is zein and soluble starch, and its weight proportion is: zein 50%, and soluble starch 50%, the weight of carrier and bacterium liquid is 1: 0.6.Mix 30～40 ℃ of fluidized dryings in back and make enterococcus bacterium powder.Cultivate used culture medium and the condition of culture of rejuvenation according to flat board, the viable bacteria content of bacterium powder is carried out conventional plate count detect,, utilize carrier that viable bacteria is diluted to 2 * 10 respectively according to count results
10Cfu/g, the vehicle weight proportioning is: zein 5%, soluble starch 95%.
Embodiment 4～18: the preparation of microorganism vitamin premixed material
The preparation method of F1 among the embodiment 4:
(1) press the described proportioning of table 1 and table 2, take by weighing each raw material quality, 100kg is an example with preparation, and the material quantity that needs is: bacillus subtilis 2.5kg, S. cervisiae 2.5kg, lactoenterococcus 2.5kg, vitamin A 0.48kg, vitamin D
30.09kg, vitamin e1 .28kg, vitamin K
30.1kg, Cobastab
10.058kg, Cobastab
20.33kg, Cobastab
60.066kg, Cobastab
120.08kg, glucose total amount 90.016kg, a copy of it is 2.484kg, another part is 87.522kg;
(2) the carrier glucose 2.484kg with vitamin raw materials and equivalent puts into the efficient horizontal mixer container stirring of twin shaft 2 minutes, mixes;
(3) add bacterium and another part glucose 87.522kg, continue to stir 5 minutes, mix discharging and can obtain microorganism vitamin premixed material product F 1;
The preparation method of F2 among the embodiment 5:
(1) press the described proportioning of table 1 and table 2, take by weighing each raw material quality, 100kg is an example with preparation, and the material quantity that needs is: bacillus licheniformis 10kg, candida utili bacterium 20kg, enterococcus faecalis 10kg, vitamin A 0.35kg, vitamin D
30.066kg, vitamin E 0.94kg, vitamin K
30.076kg, Cobastab
10.043kg, Cobastab
20.24kg, Cobastab
60.049kg, Cobastab
120.058kg, carrier total amount 58.178kg, wherein carrier is the mixture of maize cob meal, wheat bran, powdered rice hulls, a copy of it is 1.822kg, another part is 56.356kg;
(2) with vitamin raw materials and etc. the carrier 1.822kg of weight put into " V " type mixer container and stirred 10 minutes, mix;
(4) add bacterium and another part carrier 56.356kg, continue to stir 25 minutes, mix discharging and can obtain microorganism vitamin premixed material product F 2;
The preparation method of F3 among the embodiment 6:
(1) press the described proportioning of table 1 and table 2, take by weighing each raw material quality, 100kg is an example with preparation, and the material quantity that needs is:
Bacillus subtilis 5kg, bacillus licheniformis 5kg, S. cervisiae 10kg, candida utili bacterium 10kg, enterococcus faecalis 5kg, VREF 5kg, lactoenterococcus 10kg, vitamin A 0.67kg, vitamin D
30.098kg, vitamin E2 .68kg, vitamin K
30.1kg, Cobastab
10.049kg, Cobastab
20.25kg, Cobastab
60.13kg, Cobastab
120.056kg, the wheat bran total amount is 45.967kg, a copy of it is 4.033kg, another part is 41.934kg;
(2) with vitamin raw materials and etc. the carrier wheat bran 4.033kg of weight, put into horizontal paddle mixer container to stir 25 minutes, mix;
(3) add bacterium powder raw material and another part wheat bran 41.934kg and continue to stir 45 minutes, mix discharging and can obtain microorganism vitamin premixed material product F 3;
The preparation method of embodiment 7～18 roughly the same.
The animal feeding efficacy test of embodiment 19 product microorganism vitamin premixed material products of the present invention (F1)
Select for use the white x Da Bai of length about body weight 9kg to hybridize 150 of weanling pigs, the date of birth differs and is no more than 7 days.Be divided into sex ratio at random according to body weight size, sex ratio and be divided into five groups at random, 30 every group, experimental period 30d.Wherein control group fed ordinary maize-dregs of beans type daily ration does not add microorganism vitamin premixed material.Test group I is for adding the additive for microbe feedstuff that product F 1 of the present invention is removed vitamin ingredients on basal diet, test group II is for adding the antibiotic that the 100g/T prevention is had loose bowels on basal diet; Test group III adds 5 ‰ microorganism premix products (F1) on basal diet; Test group IV adds multidimensional premix.
Table 3 is fed, and basal diet is formed and trophic level
| Daily ration is formed | Content (%) | Nutritive index | Trophic level |
| Corn | 66 | Digestible energy (MJ/kg) | 13.23 |
| Wheat bran | 3 | Crude protein (%) | 18.1 |
| Dregs of beans | 17 | Calcium (%) | 0.86 |
| Fry beans | 5 | Available phosphorus (%) | 0.52 |
| Fish meal | 5 | Lysine (%) | 0.91 |
The influence of 1 pair of piglet production performance of table 4 microorganism vitamin premixed material product F
| Group | Beginning counterpoise (kg) | End counterpoise (kg) | Equal daily gain (g/ head) | Average feed consumption (the g/ head. day) | Feedstuff-meat ratio | Diarrhea rate (%) |
| Control group | 8.8±0.42 | 23.6±1.3 2 | 493±21 | 856±45 | 1.74±0.0 8 | 10.7±0.6 |
| Test group I | 8.7±0.37 | 24.5±1.4 7 | 526±35 | 851±39 | 1.61±0.0 7 | 3.5±0.3 |
| Test group II | 8.9±0.45 | 24.8±1.4 3 | 530±31 | 873±42 | 1.65±0.0 9 | 5.6±0.5 |
| Test group III | 8.8±0.40 | 25.0±1.5 0 | 540±40 | 864±51 | 1.60±0.1 0 | 3.2±0.4 |
| Test group IV | 8.8±0.42 | 24.0±1.4 0 | 506±33 | 852±42 | 1.68±0.0 7 | 7.5±0.3 |
From table 4 data, adding product microorganism vitamin premixed material product of the present invention (F1) all improves a lot than control group in average daily gain and aspect the price of deed, all daily gain has improved 8.7%, and feedstuff-meat ratio has reduced by 8.0%, and diarrhea rate has reduced by 70%.The while effect also is better than only adds additive for microbe feedstuff or only adds multidimensional premix.Shown that fully product of the present invention can promote piglet digestion, tachyauxesis, prevention diarrhoea shortens breeding cycle, reduces feeding cost.
Embodiment 20 products of the present invention (F2, F3, F4) are at the nursing effect of breeding growing and fattening pigs
Select for use and select to grow totally 300 of the close Landraces of normal, healthy body weight, be divided into 5 groups.The control group fed basal diet, test group I adds 0.5% microorganism vitamin premixed material product (F2) on basal diet, test group II adds 0.5% microorganism vitamin premixed material product (F3) on basal diet, test group III adds 0.5% microorganism vitamin premixed material product (F4) on basal diet, test group IV adds the 100g/T antibiotic on basal diet.
Result of the test shows that each growth phase average daily gain of pig has improved 8.7～10.1%, and feedstuff-meat ratio has reduced by 7.5～9.6%.Economic benefit has improved 14.5～18.5% than control group.Every production performance is not less than adds antibiotic test group, and the pig house stink reduces, and breeding environment improves.
The nursing efficacy test of embodiment 21 product microorganism vitamin premixed material products of the present invention (F6)
This test select for use 60 age in week 3000 of laying hens, be divided into 2 test group at random, 1500 of each test group.In basal diet, add 0.5% microorganism vitamin premixed material product F 5 respectively.
The influence of 6 pairs of laying hen production performances of table 5 microorganism premix product F
| Group | Egg size g/ | Day consumption g/ | Feedstuff-egg ratio | Laying rate % | Breakage rate % | Death rate % |
| Only | ||||||
| Test group | 55.61 | 124.6 | 2.24 | 72.58 | 0.23 | 0.2 |
| Control group | 55.56 | 126.1 | 2.27 | 70.79 | 0.21 | 0.5 |
The data of table 5 show, add the laying rate that premix product of the present invention can significantly improve laying hen, and laying rate improves 2.53%; Because premix product of the present invention can be regulated the gut flora balance, improved efficiency of feed utilization, reduced the price of deed; The interpolation of this product had both improved the immunity of body, had replenished vitamin again, reduced the generation of disease, had reduced the death rate; Simultaneously, reduce the ammonia in the pouity dwelling place and the content of hydrogen sulfide gas, improved breeding environment.
Embodiment 22～23 microorganism vitamin premixed materials of the present invention are to the influence of chick, fryer
Table 6
| Embodiment number | Premix | Test method | Experimental scale | Result of the test |
| 22 | F5 | The A group is control group, and the B group is for adding 0.5% F5, and the C group is for adding the antibiotic of 100g/T. | Every group of 2000 chicks | Compare daily gain raising 5.1%, feed conversion rate raising 5.4% with control group, death rate obviously reduces by 11.2%, and effect is better than the antibiotic group, and the hen house stink obviously reduces |
| 23 | F7 | The A group is control group, and the B group is for adding 0.5% F7, and the C group is for adding the antibiotic of 100g/T. | Every group of 2000 fryer | Compare daily gain 5.6%, feed conversion rate raising 5.3% with control group, the deposition of chicken body internal protein obviously increases, and death rate reduces by 9.8%, and effect is not less than adds the antibiotic group, and the hen house stink obviously reduces |
The influence of embodiment 24～26 premix product F 9 of the present invention, F10, F11 to calf, milk cow, stocker
Table 7
| Embodiment number | Premix | Test method | Experimental scale | Result of the test |
| 24 | F9 | Test group 1: organize the basal diet test group 2 of only feeding in contrast: the product F of the present invention 9 of interpolation 0.5% except that basal diet | Every group 30 dates of birth are close, differ and are no more than 7 days calf | Compare daily gain, feed conversion rate raising 6.0% with control group, death rate obviously reduces by 55%. |
| 25 | F10 | Test group A: be control group, the beef cattle basal diet B that only feeds group is added 0.5% F10 except that the basal diet of feeding | Every group of 20 body weight, close, the stocker that the date of birth is close | Compare daily gain raising 4.7%, feed conversion rate raising 5.2% with control group. |
| 26 | F11 | Test group 1: organize in contrast, only feeding cow basal diet test group 2: except that the feeding cow basal diet, the amount according to 0.5% is added product F 11 of the present invention | The milk cow that the selective body heavy phase is near, parity is identical is divided into 2 groups, 30 every group | Compare with control group, improve between 6～10% in the peak period daily yielding of giving milk, and obviously slow down in the later stage of the giving milk speed that this product output of milk descends of feeding, the incidence of mastitis for milk cows will hang down 10.5%.Feed conversion rate improves 5.5%. |
The effect of embodiment 27 product F 12 of the present invention
2 close areas of water quality, the pond that size is close, the main carp fingerling of supporting.Wherein the 1# pond is contrast, 2# pond test products F12, and product addition of the present invention is 0.5%.
Table 8 uses the influence of product of the present invention to the fingerling growing state
| Grouping | Throw in mantissa | Results mantissa | Survival rate (%) | Starting weight (kg) | End heavy (kg) | Net gain (kg) | Feed consumption total amount (kg) | Feedstuff-meat ratio |
| 1 | 9000 | 6348 | 70.5 | 50.0 | 1258.0 | 1208.0 | 2607.5 | 2.16 |
| 2 | 9000 | 6760 | 75.1 | 50.0 | 1438.5 | 1388.5 | 2606.8 | 1.88 |
As can be seen from Table 8, use microorganism vitamin premixed material F12 of the present invention, for the survival rate raising 6.5% of carp fingerling, feedstuff-meat ratio is reduced to 1.88 by 2.16.
The effect of embodiment 28 product F 13 of the present invention, F14
3 close, big or small close ponds of area of water quality, main foster carp.Wherein the 1# pond is contrast, 2# pond test products F13,3# pond test products F14.Product addition of the present invention is 0.5%.
Table 9 uses the influence of product of the present invention to the adult fish growing state
| Grouping | Throw in mantissa | Results mantissa | Survival rate (%) | Starting weight (kg) | End heavy (kg) | Net gain (kg) | Feed consumption total amount (kg) | Feedstuff-meat ratio |
| 1 | 2000 | 1852 | 92.6 | 300.5 | 1706.9 | 1406.9 | 3236.0 | 2.3 |
| 2 | 2000 | 1908 | 95.4 | 300.0 | 1812.0 | 1512.0 | 3176.0 | 2.1 |
| 3 | 2000 | 1922 | 96.1 | 300.0 | 1818.8 | 1506.8 | 3165.0 | 2.1 |
As can be seen from Table 8, use microorganism vitamin premixed material F13 of the present invention, F14, for the survival rate raising 3.0～3.8% of adult fish, feedstuff-meat ratio is reduced to 2.1 by 2.3.
Claims (2)
1. microorganism vitamin premixed material, it is characterized in that comprising bacillus, saccharomycete and enterococcus bacterium powder, vitamin and carrier, weight (wt) proportioning of bacillus, saccharomycete and enterococcus bacterium powder is 1.5～60%, the weight of bacillus (wt) proportioning is 0.5～20%, saccharomycetic weight (wt) proportioning is 0.5～20%, enterococcal weight (wt) proportioning is 0.5～20%, and the weight of vitamin (wt) proportioning is 0.58～6.64%; Described bacillus is one or both in bacillus subtilis, the bacillus licheniformis; Described saccharomycete is one or both in saccharomyces cerevisiae, the candida utili; Described enterococcus is one or more in enterococcus faecalis, VREF, the lactoenterococcus; Described vitamin comprises vitamin A, vitamin D
3, vitamin E, vitamin K
3, Cobastab
1, Cobastab
2, Cobastab
6And Cobastab
12, weight (wt) proportioning is: vitamin A 0.02～0.85%, vitamin D
30.01～0.25%, vitamin E 0.5～3.6%, vitamin K
30.01 Cobastab～0.8%,
10.01 Cobastab～0.35%,
20.01 Cobastab～0.44%,
60.01 Cobastab～0.26%,
120.01～0.09%; Described carrier is any one or more in glucose, lactose, sucrose, soluble starch, wheat bran, defatted rice bran, powdered rice hulls, maize cob meal, corn protein powder, the stone flour.
2. microorganism vitamin premixed material as claimed in claim 1 is characterized in that it is that pig is used universal microorganism vitamin premixed material with universal microorganism vitamin premixed material, ruminant with universal microorganism vitamin premixed material or aquatic livestock with universal microorganism vitamin premixed material, fowl.
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