CN101328089B - Method for producing composite microorganism phosphate using low grade ground phosphate rock - Google Patents
Method for producing composite microorganism phosphate using low grade ground phosphate rock Download PDFInfo
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- CN101328089B CN101328089B CN2008100485312A CN200810048531A CN101328089B CN 101328089 B CN101328089 B CN 101328089B CN 2008100485312 A CN2008100485312 A CN 2008100485312A CN 200810048531 A CN200810048531 A CN 200810048531A CN 101328089 B CN101328089 B CN 101328089B
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Abstract
The invention relates to a method for producing a compound microorganism phosphate fertilizer using a low grade rock phosphate powder which comprises the following steps: 1) preparing a first bacteria solution of candidakrissii and a first bacteria solution of acidithiobacillus ferrooxidans; 2) preparing a secondary bacteria solution of candidakrissii and a secondary bacteria solution of acidithiobacillus ferrooxidans; 3) preparing the compound microorganism phosphate fertilizer accoring to the following weight percentage: (1) rice, wheat or corn stalk 20-30%, (2) ammonium sulphate 5-10%, (3) pyrite powder(200-300 mesh) 10-20%, (4) lowgrade phosphate rock(200-300 mesh) 10-20%, (5) the secondary bacteria solution of candidakrissii 15-20%, (6) the secondary bacteria solution of acidithiobacillus ferrooxidans 15-20%. The method has advantage of low cost and can improve the phosphate fertilizer utilization efficiency.
Description
Technical field
A kind of method of utilizing low grade rock phosphate to produce complex microorganism phosphate fertilizer of the present invention.
Background technology
Phosphorus is one of most important nutritive element of plant, and for a long time, phosphate fertilizer is being brought into play important effect aspect the raising crop yield.The production of tradition phosphate fertilizer generally is to utilize inorganic acid solution ground phosphate rock such as sulfuric acid, by sulfuric acid ground phosphate rock is carried out chemistry and ferments and form.But the phosphate fertilizer utilization efficiency that this method is produced is very low, and nearly 80% above phosphoric is not absorbed by crop, but by soil fixing, has therefore caused the significant wastage of resource and the raising of production cost, has also caused huge environmental pollution simultaneously.
China is a phosphate rock resource big country, but major part all is a low-grade phosphate ore, the content of Vanadium Pentoxide in FLAKES is only directly used and show certain effect on acid soil and some crop below 20%, and relevant with disseminated grain size, complicacy and the grade of phosphorus ore thing in the ore.At present, to be processed into the cost of high concentration phosphate fertilizer very high because these low-grade phosphate ores are carried out ore dressing and enrichment, and many low-grade phosphate ore resources all go out of use, and do not obtain effective development and utilization.
In recent years, along with the new demand of development of biology and Green Chemistry, the microbial leaching technology has been brought into play more and more enormous function aspect low-grade, difficult, the complex ore handling.Utilization have a liking for chemosynthetic autotroph microorganism such as the ferrous thiobacillus of acid oxidase handle complicated copper mine, gold mine extracts on the metals such as copper, gold and has obtained industrial application.At present, utilizing microbial leaching technical finesse phosphorus ore to produce aspect the microorganism phosphate fertilizer, a small amount of related invention patent is also arranged, but mostly these patents are to utilize the chmosynthetic heterotrophs microorganism both at home and abroad, the culture condition of microorganism is required harsh relatively, simultaneously also indirect raising production cost.In addition, at present especially the patent of invention at the development and use of low-grade phosphate ore resource does not have substantially, produce complex microorganism phosphate fertilizer if can fully utilize chmosynthetic heterotrophs microorganism and chemosynthetic autotroph microbiological treatment low-grade phosphate ore, the deficiency of related invention patent before so just can remedying, improve p fertilizer efficiency, can make the low-grade phosphate ore resource of China's abundant obtain sufficient development and utilization again simultaneously.
Summary of the invention
The object of the present invention is to provide a kind of method of utilizing low grade rock phosphate to produce complex microorganism phosphate fertilizer, this method production cost is low, can improve phosphate fertilizer utilization efficiency.
To achieve these goals, technical scheme of the present invention is: a kind of method of utilizing low grade rock phosphate to produce complex microorganism phosphate fertilizer is characterized in that it comprises the steps:
1) Crius candiyeast (Candida krissii) (all Crius candiyeasts all are applicable to the present invention) is inoculated in the Crius candiyeast one-level substratum that pH is 2.5-3.0, the volume of Crius candiyeast is the 10-20% of Crius candiyeast one-level culture volume, place constant temperature shaking table shaking culture, rotating speed 160-180 rev/min, culture temperature 28-32 ℃, cultivate after 4-6 days, filter and collection filtrate, be the one-level bacterium liquid of Crius candiyeast;
To have a liking for the ferrous thiobacillus of acid oxidase (Acidithiobacillus ferrooxidans, be called for short At.f) (all ferrous thiobacilluss of acid oxidase of having a liking for all are applicable to the present invention) be inoculated in pH be 2.5-3.0 have a liking for the ferrous thiobacillus one-level of acid oxidase substratum, the volume of having a liking for the ferrous thiobacillus of acid oxidase is the 10-20% that has a liking for the ferrous thiobacillus one-level of acid oxidase culture volume, place constant temperature shaking table shaking culture, rotating speed 160-180 rev/min, culture temperature 28-32 ℃, cultivate after 4-6 days, filter and collection filtrate, be the one-level bacterium liquid of having a liking for the ferrous thiobacillus of acid oxidase;
Described Crius candiyeast one-level nutrient media components:
Glucose 10g,
(NH
4)
2SO
4 0.15g,
KCl 0.2g,
MgCl?
2·6H
2O 0.5g,
MgSO
4·7H
2O 0.25g,
Ca
3(PO
4)
2 5g,
Distilled water 1000mL;
Describedly have a liking for the ferrous thiobacillus one-level of acid oxidase nutrient media components:
(NH
4)
2SO
4 3g,
KCl 0.1g,
K
2HPO
4 0.5g,
MgSO
4·7H
2O 0.5g,
Ca(NO
3)
2 0.01g,
FeSO
4·7H
2O 44.2g,
Ca
3(PO
4)
2 5g,
Distilled water 1000mL;
2) the one-level bacterium liquid with the Crius candiyeast is inoculated in the Crius candiyeast secondary medium that pH is 2.5-3.0, and the volume of Crius candiyeast one-level bacterium liquid is the 10-20% of Crius candiyeast secondary medium volume; Place constant temperature shaking table shaking culture, rotating speed 160-180 rev/min, culture temperature 28-32 ℃, cultivate after 4-6 days, filter and collection filtrate, be the secondary bacterium liquid of Crius candiyeast;
The one-level bacterium liquid that to have a liking for the ferrous thiobacillus of acid oxidase be inoculated in pH be 2.5-3.0 have a liking for the ferrous thiobacillus secondary medium of acid oxidase, the volume of having a liking for the one-level bacterium liquid of the ferrous thiobacillus of acid oxidase is the 10-20% that has a liking for the ferrous thiobacillus secondary medium of acid oxidase volume; Place constant temperature shaking table shaking culture, rotating speed 160-180 rev/min, culture temperature 28-32 ℃, cultivate after 4-6 days, filter and collection filtrate, be the secondary bacterium liquid of having a liking for the ferrous thiobacillus of acid oxidase;
Described Crius candiyeast secondary medium component:
Glucose 5-15g,
(NH
4)
2SO
4 0.1-0.3g,
KCl 0.1-0.3g,
MgCl?
2·6H
2O 0.5-1.0g,
MgSO
4·7H
2O 0.1-0.5g,
Low grade rock phosphate (200-300 order) 5-15g,
Distilled water 1000mL;
Describedly have a liking for the ferrous thiobacillus secondary medium of acid oxidase component:
(NH
4)
2SO
4 1-5g,
KCl 0.1-0.3g,
K
2HPO
4 0.1-1.0g,
MgSO
4·7H
2O 0.1-1.0g,
Ca(NO
3)
2 0.01-0.5g,
Pyritic ashes (200-300 order) 10-30g,
Low grade rock phosphate (200-300 order) 5-15g,
Distilled water 1000mL;
3) by following mass percent proportioning:
Paddy rice, wheat or maize straw 20-30%,
Ammonium sulfate 5-10%,
Pyritic ashes (200-300 order) 10-20%,
Low grade rock phosphate (200-300 order) 10-20%,
The secondary bacterium liquid 15-20% of Crius candiyeast,
Have a liking for the secondary bacterium liquid 15-20% of the ferrous thiobacillus of acid oxidase;
Mix after choosing raw material, regulating pH with volumetric concentration 10-20% sulfuric acid is 2.5-3.0, is transferred to fermentor tank and cultivates, and leavening temperature 28-32 ℃, the air flow in the fermentor tank is by feeding with fermentation volume 1: 1-1.5 (volume ratio), and tank pressure is 0.5-1kg/cm
2, stirring velocity is 160-180 rev/min, ferments after 4-6 days, packing is made complex microorganism phosphate fertilizer.
The mass content of the Vanadium Pentoxide in FLAKES in the described low grade rock phosphate is lower than 20%.
Described paddy rice, wheat or maize straw are: the paddy rice after will gathering in the crops, wheat or maize straw are through becoming thoroughly decomposed, pulverize, cross 5-10mm aperture sieve, oven dry, and the cooling back is as the required carbon source material of Crius candiyeast growth.The ammonium sulfate that to buy by Chemical market is as the required nitrogen source of Crius candiyeast growth.To have a liking for the required energy substance of the ferrous growth of thiobacillus of acid oxidase by the pyritic ashes conduct that market is bought or gather in the mine.
Described Crius candiyeast is gram-negative chmosynthetic heterotrophs microorganism, and having a liking for the ferrous thiobacillus of acid oxidase is gram-negative chemosynthetic autotroph microorganism.
Described bacterial classification is that a strain has the thiobacillus that molten phosphorus fungi, a strain of dissolving the inorganic phosphorus function have oxidation pyritic ashes generation sulfuric acid function.Molten phosphorus fungi with dissolving inorganic phosphorus function is the Crius candiyeast, according to experiment showed, that repeatedly this microorganism can have an effect with ground phosphate rock, and the secretion organic acid, thus dissolve titanium pigment in the ground phosphate rock.Have thiobacillus that the oxidation pyritic ashes produces the sulfuric acid function for having a liking for the ferrous thiobacillus of acid oxidase, according to experiment showed, that repeatedly this microorganism can oxidation pyritic ashes generation sulfuric acid, thereby dissolve the titanium pigment in the ground phosphate rock.
The invention has the beneficial effects as follows:
1, the complex microorganism phosphate fertilizer that utilizes this method to produce can make the available phosphorus content in the soil increase 20-30%, and phosphate fertilizer utilization efficiency improves more than 15%.
2, the phosphate fertilizer of this method production is applicable to various soil and farm crop, and is without phosphorus residual.
3, this method can be utilized the low-grade phosphate ore resource of China's abundant comparatively fully, reduces the production of phosphate fertilizer cost simultaneously, and China's agricultural development and the improvement of phosphate fertilizer industrial environment are had important practical significance.
Embodiment
In order to understand the present invention better, further illustrate content of the present invention below in conjunction with embodiment, but content of the present invention not only is confined to the following examples.
Embodiment 1:
A kind of method of utilizing low grade rock phosphate to produce complex microorganism phosphate fertilizer, it comprises the steps:
1), 1. the Crius candiyeast (Candida krissii) of 10mL is inoculated in the Crius candiyeast one-level substratum (being that 20% sulfuric acid adjusting Crius candiyeast one-level medium pH is 2.5 with volumetric concentration in advance) of 90mL by transfer pipet in Bechtop, place constant temperature shaking table shaking culture, 180 rev/mins of rotating speeds, 30 ℃ of culture temperature, cultivate after 6 days, filter and collection filtrate, be the one-level bacterium liquid of Crius candiyeast;
Described Crius candiyeast one-level substratum (NBRIP substratum) component:
Glucose 10g,
(NH
4)
2SO
4 0.15g,
KCl 0.2g,
MgCl?
2·6H
2O 0.5g,
MgSO
4·7H
2O 0.25g,
Ca
3(PO
4)
2 5g,
Distilled water 1000mL;
2. 10mL had a liking for the ferrous thiobacillus of acid oxidase (Acidithiobacillus ferrooxidans, be called for short At.f) in Bechtop by transfer pipet be inoculated in 90 have a liking for the ferrous thiobacillus one-level of acid oxidase substratum (being that to have a liking for the ferrous thiobacillus one-level of acid oxidase medium pH be 2.5 to the adjusting of 20% sulfuric acid with volumetric concentration in advance), place constant temperature shaking table shaking culture, 180 rev/mins of rotating speeds, 30 ℃ of culture temperature, cultivate after 6 days, filter and collection filtrate, be the one-level bacterium liquid of having a liking for the ferrous thiobacillus of acid oxidase;
Describedly have a liking for the ferrous thiobacillus one-level of acid oxidase substratum (9K substratum) component:
(NH
4)
2SO
4 3g,
KCl 0.1g,
K
2HPO
4 0.5g,
MgSO
4·7H
2O 0.5g,
Ca(NO
3)
2 0.01g,
FeSO
4·7H
2O 44.2g,
Ca
3(PO
4)
2 5g,
Distilled water 1000mL;
2), 1. the one-level bacterium liquid of the Crius candiyeast of 10mL is inoculated in the Crius candiyeast secondary medium (being that 20% sulfuric acid adjusting Crius candiyeast secondary medium pH is 2.5 with volumetric concentration earlier) of 90mL by transfer pipet in Bechtop, place constant temperature shaking table shaking culture, 180 rev/mins of rotating speeds, 30 ℃ of culture temperature, cultivate after 6 days, filter and collection filtrate, be the secondary bacterium liquid of Crius candiyeast;
Described Crius candiyeast secondary medium (the NBRIP substratum of modification) component:
Glucose 10g,
(NH
4)
2SO
4 0.15g,
KCl 0.2g,
MgCl
2·6H
2O 0.5g,
MgSO
4·7H
2O 0.25g,
Low grade rock phosphate (200 order) 5g,
Distilled water 1000mL;
2. the one-level bacterium liquid of having a liking for the ferrous thiobacillus of acid oxidase of 10mL is had a liking for the ferrous thiobacillus secondary medium of acid oxidase (being that to have a liking for the ferrous thiobacillus secondary medium of acid oxidase pH be 2.5 to the adjusting of 20% sulfuric acid with volumetric concentration earlier) by what transfer pipet was inoculated in 90mL in Bechtop, place constant temperature shaking table shaking culture, 180 rev/mins of rotating speeds, 30 ℃ of culture temperature, cultivate after 6 days, filter and collection filtrate, be the secondary bacterium liquid of having a liking for the ferrous thiobacillus of acid oxidase;
Describedly have a liking for the ferrous thiobacillus secondary medium of acid oxidase (the 9K substratum of modification) component:
(NH
4)
2SO
4 3g,
KCl 0.1g,
K
2HPO
4 0.5g,
MgSO
4·7H
2O 0.5g,
Ca(NO
3)
2 0.01g,
Pyritic ashes (200 order) 10g,
Low grade rock phosphate (200 order) 5g,
Distilled water 1000mL;
3) by following mass percent proportioning:
Rice straw 20%,
Ammonium sulfate 10%,
Pyritic ashes (200 order) 20%,
Low grade rock phosphate (200 order) 15%,
The secondary bacterium liquid 17.5% of Crius candiyeast,
Have a liking for the secondary bacterium liquid 17.5% of the ferrous thiobacillus of acid oxidase;
Mix after choosing raw material, regulating pH with volumetric concentration 20% sulfuric acid is 2.5, be transferred to fermentor tank and cultivate, and 30 ℃ of leavening temperatures, the air flow in the fermentor tank is by feeding with fermentation volume 1: 1.5 (volume ratio), and tank pressure is 0.5kg/cm
2, stirring velocity is 180 rev/mins, ferments after 6 days, promptly packing is made complex microorganism phosphate fertilizer.
The mass content of the Vanadium Pentoxide in FLAKES in the described low grade rock phosphate of present embodiment is 19.9%.
Embodiment 2:
A kind of method of utilizing low grade rock phosphate to produce complex microorganism phosphate fertilizer, it comprises the steps:
1), 1. the Crius candiyeast (Candida krissii) of 15mL is inoculated in the Crius candiyeast one-level substratum (being that 20% sulfuric acid adjusting Crius candiyeast one-level medium pH is 2.8 with volumetric concentration earlier) of 100mL by transfer pipet in Bechtop, place constant temperature shaking table shaking culture, 160 rev/mins of rotating speeds, 32 ℃ of culture temperature, cultivate after 5 days, filter and collection filtrate, be the one-level bacterium liquid of Crius candiyeast;
Described Crius candiyeast one-level substratum (NBRIP substratum) component:
Glucose 10g,
(NH
4)
2SO
4 0.15g,
KCl 0.2g,
MgCl
2·6H
2O 0.5g,
MgSO
4·7H
2O 0.25g,
Ca
3(PO
4)
2 5g,
Distilled water 1000mL;
2. 15mL had a liking for the ferrous thiobacillus of acid oxidase (Acidithiobacillus ferrooxidans, be called for short At.f) in Bechtop, have a liking for the ferrous thiobacillus one-level of acid oxidase substratum (being that to have a liking for the ferrous thiobacillus one-level of acid oxidase medium pH be 2.8 to the adjusting of 20% sulfuric acid with volumetric concentration earlier) by what transfer pipet was inoculated in 100mL, place constant temperature shaking table shaking culture, 160 rev/mins of rotating speeds, 32 ℃ of culture temperature, cultivate after 5 days, filter and collection filtrate, be the one-level bacterium liquid of having a liking for the ferrous thiobacillus of acid oxidase;
Describedly have a liking for the ferrous thiobacillus one-level of acid oxidase substratum (9K substratum) component:
(NH
4)
2SO
4 3g,
KCl 0.1g,
K
2HPO
4 0.5g,
MgSO
4·7H
2O 0.5g,
Ca(NO
3)
2 0.01g,
FeSO
4·7H
2O 44.2g,
Ca
3(PO
4)
2 5g,
Distilled water 1000mL;
2), 1. the one-level bacterium liquid of the Crius candiyeast of 15mL is inoculated in the Crius candiyeast secondary medium (being that 20% sulfuric acid adjusting Crius candiyeast secondary medium pH is 2.8 with volumetric concentration earlier) of 100mL by transfer pipet in Bechtop, place constant temperature shaking table shaking culture, 160 rev/mins of rotating speeds, 32 ℃ of culture temperature, cultivate after 5 days, filter and collection filtrate, be the secondary bacterium liquid of Crius candiyeast;
Described Crius candiyeast secondary medium (the NBRIP substratum of modification) component:
Glucose 15g,
(NH
4)
2SO
4 0.2g,
KCl 0.15g,
MgCl
2·6H
2O 0.5g,
MgSO
4·7H
2O 0.2g,
Low grade rock phosphate (250 order) 10g,
Distilled water 1000mL;
2. the one-level bacterium liquid of having a liking for the ferrous thiobacillus of acid oxidase of 15mL is had a liking for the ferrous thiobacillus secondary medium of acid oxidase (being that to have a liking for the ferrous thiobacillus secondary medium of acid oxidase pH be 2.8 to the adjusting of 20% sulfuric acid with volumetric concentration earlier) by what transfer pipet was inoculated in 100mL in Bechtop, place constant temperature shaking table shaking culture, 160 rev/mins of rotating speeds, 32 ℃ of culture temperature, cultivate after 5 days, filter and collection filtrate, be the secondary bacterium liquid of having a liking for the ferrous thiobacillus of acid oxidase;
Describedly have a liking for the ferrous thiobacillus secondary medium of acid oxidase (the 9K substratum of modification) component:
(NH
4)
2SO
4 5g,
KCl 0.2g,
K
2HPO
4 0.1g,
MgSO
4·7H
2O 0.5g,
Ca(NO
3)
2 0.05g,
Pyritic ashes (250 order) 25g,
Low grade rock phosphate (250 order) 10g,
Distilled water 1000mL;
3) by following mass percent proportioning:
Wheat stalk 30%,
Ammonium sulfate 10%,
Pyritic ashes (250 order) 20%,
Low grade rock phosphate (250 order) 10%,
The secondary bacterium liquid 15% of Crius candiyeast,
Have a liking for the secondary bacterium liquid 15% of the ferrous thiobacillus of acid oxidase;
Mix after choosing raw material, regulating pH with volumetric concentration 20% sulfuric acid is 2.8, be transferred to fermentor tank and cultivate, and 32 ℃ of leavening temperatures, the air flow in the fermentor tank is by feeding with fermentation volume 1: 1 (volume ratio), and tank pressure is 1kg/cm
2, stirring velocity is 160 rev/mins, ferments after 5 days, promptly packing is made complex microorganism phosphate fertilizer.
The mass content of the Vanadium Pentoxide in FLAKES in the described low grade rock phosphate of present embodiment is 18%.
Embodiment 3:
A kind of method of utilizing low grade rock phosphate to produce complex microorganism phosphate fertilizer, it comprises the steps:
1) the Crius candiyeast (Candida krissii) with 20mL is inoculated in 150mL Crius candiyeast one-level substratum (being that 10% sulfuric acid adjusting Crius candiyeast one-level medium pH is 3.0 with volumetric concentration earlier) by transfer pipet in Bechtop, place constant temperature shaking table shaking culture, 170 rev/mins of rotating speeds, 28 ℃ of culture temperature, cultivate after 4 days, filter and collection filtrate, be the one-level bacterium liquid of Crius candiyeast;
Described Crius candiyeast one-level substratum (NBRIP substratum) component:
Glucose 10g,
(NH
4)
2SO
4 0.15g,
KCl 0.2g,
MgCl
2·6H
2O 0.5g,
MgSO
4·7H
2O 0.25g,
Ca
3(PO
4)
2 5g,
Distilled water 1000mL;
20mL had a liking for the ferrous thiobacillus of acid oxidase (Acidithiobacillus ferrooxidans, be called for short At.f) in Bechtop, be inoculated in 150mL and have a liking for the ferrous thiobacillus one-level of acid oxidase substratum (being that to have a liking for the ferrous thiobacillus one-level of acid oxidase medium pH be 3.0 to the adjusting of 10% sulfuric acid with volumetric concentration earlier) by transfer pipet, place constant temperature shaking table shaking culture, 170 rev/mins of rotating speeds, 28 ℃ of culture temperature, cultivate after 4 days, filter and collection filtrate, be the one-level bacterium liquid of having a liking for the ferrous thiobacillus of acid oxidase;
Describedly have a liking for the ferrous thiobacillus one-level of acid oxidase substratum (9K substratum) component:
(NH
4)
2SO
4 3g,
KCl 0.1g,
K
2HPO
4 0.5g,
MgSO
4·7H
2O 0.5g,
Ca(NO
3)
2 0.01g,
FeSO
4·7H
2O 44.2g,
Ca
3(PO
4)
2 5g,
Distilled water 1000mL;
2) the one-level bacterium liquid with the Crius candiyeast of 20mL is inoculated in 150mL Crius candiyeast secondary medium (being that 10% sulfuric acid adjusting Crius candiyeast secondary medium pH is 3.0 with volumetric concentration earlier), place constant temperature shaking table shaking culture, 170 rev/mins of rotating speeds, 28 ℃ of culture temperature, cultivate after 4 days, filter and collection filtrate, be the secondary bacterium liquid of Crius candiyeast;
Described Crius candiyeast secondary medium (the NBRIP substratum of modification) component:
Glucose 5g,
(NH
4)
2SO
4 0.1g,
KCl 0.3g,
MgCl
2·6H
2O 1.0g,
MgSO
4·7H
2O 0.5g,
Low grade rock phosphate (300 order) 5g,
Distilled water 1000mL;
The one-level bacterium liquid of having a liking for the ferrous thiobacillus of acid oxidase of 20mL is inoculated in 150mL has a liking for the ferrous thiobacillus secondary medium of acid oxidase (being that to have a liking for the ferrous thiobacillus secondary medium of acid oxidase pH be 3.0 to the adjusting of 10% sulfuric acid with volumetric concentration earlier), place constant temperature shaking table shaking culture, 170 rev/mins of rotating speeds, 28 ℃ of culture temperature, cultivate after 4 days, filter and collection filtrate, be the secondary bacterium liquid of having a liking for the ferrous thiobacillus of acid oxidase;
Describedly have a liking for the ferrous thiobacillus secondary medium of acid oxidase (the 9K substratum of modification) component:
(NH
4)
2SO
4 5g,
KCl 0.1g,
K
2HPO
4 0.5g,
MgSO
4·7H
2O 0.5g,
Ca(NO
3)
2 0.05g,
Pyritic ashes (300 order) 30g,
Low grade rock phosphate (300 order) 10g,
Distilled water 1000mL;
3) by following mass percent proportioning:
Maize straw 20%,
Ammonium sulfate 5%,
Pyritic ashes (300 order) 20%,
Low grade rock phosphate (300 order) 15%,
The secondary bacterium liquid 20% of Crius candiyeast,
Have a liking for the secondary bacterium liquid 20% of the ferrous thiobacillus of acid oxidase;
Mix after choosing raw material, regulating pH with volumetric concentration 10-20% sulfuric acid is 3.0, is transferred to fermentor tank and cultivates, and leavening temperature 28-32 ℃, the air flow in the fermentor tank is by feeding with fermentation volume 1: 1 (volume ratio), and tank pressure is 0.5-1kg/cm
2, stirring velocity is 160-180 rev/min, ferments after 4-6 days, promptly packing is made complex microorganism phosphate fertilizer.
The mass content of the Vanadium Pentoxide in FLAKES in the described low grade rock phosphate of present embodiment is 10%.
Embodiment 4:
A kind of method of utilizing low grade rock phosphate to produce complex microorganism phosphate fertilizer, it comprises the steps:
1) Crius candiyeast (Candida krissii) (all Crius candiyeasts all are applicable to the present invention) being inoculated in pH by transfer pipet in Bechtop is 2.5 Crius candiyeast one-level substratum (being that 10% sulfuric acid adjusting Crius candiyeast one-level medium pH is 2.5 with volumetric concentration earlier), the volume of Crius candiyeast is 10% of a Crius candiyeast one-level culture volume, place constant temperature shaking table shaking culture, 160 rev/mins of rotating speeds, 28 ℃ of culture temperature, cultivate after 4 days, filter and collection filtrate, be the one-level bacterium liquid of Crius candiyeast;
To have a liking for the ferrous thiobacillus of acid oxidase (Acidithiobacillus ferrooxidans, be called for short At.f) (all ferrous thiobacilluss of acid oxidase of having a liking for all are applicable to the present invention) in Bechtop by transfer pipet be inoculated in pH be 2.5 have a liking for the ferrous thiobacillus one-level of acid oxidase substratum (being that to have a liking for the ferrous thiobacillus one-level of acid oxidase medium pH be 2.5 to the adjusting of 10% sulfuric acid with volumetric concentration earlier), the volume of having a liking for the ferrous thiobacillus of acid oxidase is to have a liking for 10% of the ferrous thiobacillus one-level of acid oxidase culture volume, place constant temperature shaking table shaking culture, 160 rev/mins of rotating speeds, 28 ℃ of culture temperature, cultivate after 4 days, filter and collection filtrate, be the one-level bacterium liquid of having a liking for the ferrous thiobacillus of acid oxidase;
Described Crius candiyeast one-level nutrient media components:
Glucose 10g,
(NH
4)
2SO
4 0.15g,
KCl 0.2g,
MgCl?
2·6H
2O 0.5g,
MgSO
4·7H
2O 0.25g,
Ca
3(PO
4)
2 5g,
Distilled water 1000mL;
Describedly have a liking for the ferrous thiobacillus one-level of acid oxidase nutrient media components:
(NH
4)
2SO
4 3g,
KCl 0.1g,
K
2HPO
4 0.5g,
MgSO
4·7H
2O 0.5g,
Ca(NO
3)
2 0.01g,
FeSO
4·7H
2O 44.2g,
Ca
3(PO
4)
2 5g,
Distilled water 1000mL;
2) the one-level bacterium liquid of Crius candiyeast being inoculated in pH by transfer pipet in Bechtop is 2.5 Crius candiyeast secondary medium (being that 10% sulfuric acid adjusting pH is 2.5 with volumetric concentration earlier), and the volume of Crius candiyeast one-level bacterium liquid is 10% of a Crius candiyeast secondary medium volume; Place constant temperature shaking table shaking culture, 160 rev/mins of rotating speeds, 28 ℃ of culture temperature were cultivated after 4 days, filtered and collection filtrate, were the secondary bacterium liquid of Crius candiyeast;
The one-level bacterium liquid that to have a liking for the ferrous thiobacillus of acid oxidase in Bechtop by transfer pipet be inoculated in pH be 2.5 have a liking for the ferrous thiobacillus secondary medium of acid oxidase (being that 10% sulfuric acid adjusting pH is 2.5 with volumetric concentration earlier), the volume of having a liking for the one-level bacterium liquid of the ferrous thiobacillus of acid oxidase is to have a liking for 10% of the ferrous thiobacillus secondary medium of acid oxidase volume; Place constant temperature shaking table shaking culture, 160 rev/mins of rotating speeds, 28 ℃ of culture temperature were cultivated after 4 days, filtered and collection filtrate, were the secondary bacterium liquid of having a liking for the ferrous thiobacillus of acid oxidase;
Described Crius candiyeast secondary medium component:
Glucose 5g,
(NH
4)
2SO
4 0.1g,
KCl 0.1g,
MgCl
2·6H
2O 0.5g,
MgSO
4·7H
2O 0.1g,
Low grade rock phosphate (200 order) 5g,
Distilled water 1000mL;
Describedly have a liking for the ferrous thiobacillus secondary medium of acid oxidase component:
(NH
4)
2SO
4 1g,
KCl 0.1g,
K
2HPO
4 0.1g,
MgSO
4·7H
2O 0.1g,
Ca(NO
3)
2 0.01g,
Pyritic ashes (200 order) 10g,
Low grade rock phosphate (200 order) 5g,
Distilled water 1000mL;
3) by following mass percent proportioning:
Rice straw 20%,
Ammonium sulfate 5%,
Pyritic ashes (200 order) 20%,
Low grade rock phosphate (200 order) 20%,
The secondary bacterium liquid 15% of Crius candiyeast,
Have a liking for the secondary bacterium liquid 20% of the ferrous thiobacillus of acid oxidase;
Mix after choosing raw material, regulating pH with volumetric concentration 10% sulfuric acid is 2.5, be transferred to fermentor tank and cultivate, and 28 ℃ of leavening temperatures, the air flow in the fermentor tank is by feeding with fermentation volume 1: 1 (volume ratio), and tank pressure is 0.5kg/cm
2, stirring velocity is 160 rev/mins, ferments after 4 days, packing is made complex microorganism phosphate fertilizer.
The mass content of the Vanadium Pentoxide in FLAKES in the described low grade rock phosphate is 19%.
Embodiment 5:
A kind of method of utilizing low grade rock phosphate to produce complex microorganism phosphate fertilizer, it comprises the steps:
1) Crius candiyeast (Candida krissii) (all Crius candiyeasts all are applicable to the present invention) being inoculated in pH by transfer pipet in Bechtop is 3.0 Crius candiyeast one-level substratum (being that 20% sulfuric acid adjusting pH is 3.0 with volumetric concentration earlier), the volume of Crius candiyeast is 20% of a Crius candiyeast one-level culture volume, place constant temperature shaking table shaking culture, 180 rev/mins of rotating speeds, 32 ℃ of culture temperature, cultivate after 6 days, filter and collection filtrate, be the one-level bacterium liquid of Crius candiyeast;
To have a liking for the ferrous thiobacillus of acid oxidase (Acidithiobacillus ferrooxidans, be called for short At.f) (all ferrous thiobacilluss of acid oxidase of having a liking for all are applicable to the present invention) in Bechtop by transfer pipet be inoculated in pH be 3.0 have a liking for the ferrous thiobacillus one-level of acid oxidase substratum (being that 20% sulfuric acid adjusting pH is 3.0 with volumetric concentration earlier), the volume of having a liking for the ferrous thiobacillus of acid oxidase is to have a liking for 20% of the ferrous thiobacillus one-level of acid oxidase culture volume, place constant temperature shaking table shaking culture, 180 rev/mins of rotating speeds, 32 ℃ of culture temperature, cultivate after 6 days, filter and collection filtrate, be the one-level bacterium liquid of having a liking for the ferrous thiobacillus of acid oxidase;
Described Crius candiyeast one-level nutrient media components:
Glucose 10g,
(NH
4)
2SO
4 0.15g,
KCl 0.2g,
MgCl?
2·6H
2O 0.5g,
MgSO
4·7H
2O 0.25g,
Ca
3(PO
4)
2 5g,
Distilled water 1000mL;
Describedly have a liking for the ferrous thiobacillus one-level of acid oxidase nutrient media components:
(NH
4)
2SO
4 3g,
KCl 0.1g,
K
2HPO
4 0.5g,
MgSO
4·7H
2O 0.5g,
Ca(NO
3)
2 0.01g,
FeSO
4·7H
2O 44.2g,
Ca
3(PO
4)
2 5g,
Distilled water 1000mL;
2) the one-level bacterium liquid of Crius candiyeast being inoculated in pH by transfer pipet in Bechtop is 3.0 Crius candiyeast secondary medium (being that 20% sulfuric acid adjusting pH is 3.0 with volumetric concentration earlier), and the volume of Crius candiyeast one-level bacterium liquid is 20% of a Crius candiyeast secondary medium volume; Place constant temperature shaking table shaking culture, 180 rev/mins of rotating speeds, 32 ℃ of culture temperature were cultivated after 6 days, filtered and collection filtrate, were the secondary bacterium liquid of Crius candiyeast;
The one-level bacterium liquid that to have a liking for the ferrous thiobacillus of acid oxidase in Bechtop by transfer pipet be inoculated in pH be 3.0 have a liking for the ferrous thiobacillus secondary medium of acid oxidase (being that 20% sulfuric acid adjusting pH is 3.0 with volumetric concentration earlier), the volume of having a liking for the one-level bacterium liquid of the ferrous thiobacillus of acid oxidase is to have a liking for 20% of the ferrous thiobacillus secondary medium of acid oxidase volume; Place constant temperature shaking table shaking culture, 180 rev/mins of rotating speeds, 32 ℃ of culture temperature were cultivated after 6 days, filtered and collection filtrate, were the secondary bacterium liquid of having a liking for the ferrous thiobacillus of acid oxidase;
Described Crius candiyeast secondary medium component:
Glucose 15g,
(NH
4)
2SO
4 0.3g,
KCl 0.3g,
MgCl
2·6H
2O 1.0g,
MgSO
4·7H
2O 0.5g,
Low grade rock phosphate (300 order) 15g,
Distilled water 1000mL;
Describedly have a liking for the ferrous thiobacillus secondary medium of acid oxidase component:
(NH
4)
2SO
4 5g,
KCl 0.3g,
K
2HPO
4 1.0g,
MgSO
4·7H
2O 1.0g,
Ca(NO
3)
2 0.5g,
Pyritic ashes (300 order) 30g,
Low grade rock phosphate (300 order) 15g,
Distilled water 1000mL;
3) by following mass percent proportioning:
Wheat stalk 30%,
Ammonium sulfate 10%,
Pyritic ashes (300 order) 10%,
Low grade rock phosphate (300 order) 10%,
The secondary bacterium liquid 20% of Crius candiyeast,
Have a liking for the secondary bacterium liquid 20% of the ferrous thiobacillus of acid oxidase;
Mix after choosing raw material, regulating pH with volumetric concentration 20% sulfuric acid is 3.0, be transferred to fermentor tank and cultivate, and 32 ℃ of leavening temperatures, the air flow in the fermentor tank is by feeding with fermentation volume 1.5 (volume ratio), and tank pressure is 1kg/cm
2, stirring velocity is 180 rev/mins, ferments after 6 days, packing is made complex microorganism phosphate fertilizer.
The mass content of the Vanadium Pentoxide in FLAKES in the described low grade rock phosphate is 10%.
Claims (2)
1. a method of utilizing low grade rock phosphate to produce complex microorganism phosphate fertilizer is characterized in that it comprises the steps:
1) Crius candiyeast (Candida krissii) is inoculated in the Crius candiyeast one-level substratum that pH is 2.5-3.0, the volume of Crius candiyeast is the 10-20% of Crius candiyeast one-level culture volume, place constant temperature shaking table shaking culture, rotating speed 160-180 rev/min, culture temperature 28-32 ℃, cultivate after 4-6 days, filter and collection filtrate, be the one-level bacterium liquid of Crius candiyeast;
To have a liking for the ferrous thiobacillus of acid oxidase (Acidithiobacillus ferrooxidans) be inoculated in pH be 2.5-3.0 have a liking for the ferrous thiobacillus one-level of acid oxidase substratum, the volume of having a liking for the ferrous thiobacillus of acid oxidase is the 10-20% that has a liking for the ferrous thiobacillus one-level of acid oxidase culture volume, place constant temperature shaking table shaking culture, rotating speed 160-180 rev/min, culture temperature 28-32 ℃, cultivate after 4-6 days, filter and collection filtrate, be the one-level bacterium liquid of having a liking for the ferrous thiobacillus of acid oxidase;
Described Crius candiyeast one-level nutrient media components:
Glucose 10g,
(NH
4)
2SO
4 0.15g,
KCl 0.2g,
MgCl
2·6H
2O 0.5g,
MgSO
4·7H
2O 0.25g,
Ca
3(PO
4)
2 5g,
Distilled water 1000mL;
Describedly have a liking for the ferrous thiobacillus one-level of acid oxidase nutrient media components:
(NH
4)
2SO
4 3g,
KCl 0.1g,
K
2HPO
4 0.5g,
MgSO
4·7H
2O 0.5g,
Ca(NO
3)
2 0.01g,
FeSO
4·7H
2O 44.2g,
Ca
3(PO
4)
2 5g,
Distilled water 1000mL;
2) the one-level bacterium liquid with the Crius candiyeast is inoculated in the Crius candiyeast secondary medium that pH is 2.5-3.0, and the volume of Crius candiyeast one-level bacterium liquid is the 10-20% of Crius candiyeast secondary medium volume; Place constant temperature shaking table shaking culture, rotating speed 160-180 rev/min, culture temperature 28-32 ℃, cultivate after 4-6 days, filter and collection filtrate, be the secondary bacterium liquid of Crius candiyeast;
The one-level bacterium liquid that to have a liking for the ferrous thiobacillus of acid oxidase be inoculated in pH be 2.5-3.0 have a liking for the ferrous thiobacillus secondary medium of acid oxidase, the volume of having a liking for the one-level bacterium liquid of the ferrous thiobacillus of acid oxidase is the 10-20% that has a liking for the ferrous thiobacillus secondary medium of acid oxidase volume; Place constant temperature shaking table shaking culture, rotating speed 160-180 rev/min, culture temperature 28-32 ℃, cultivate after 4-6 days, filter and collection filtrate, be the secondary bacterium liquid of having a liking for the ferrous thiobacillus of acid oxidase;
Described Crius candiyeast secondary medium component:
Glucose 5-15g,
(NH
4)
2SO
4 0.1-0.3g,
KCl 0.1-0.3g,
MgCl
2·6H
2O 0.5-1.0g,
MgSO
4·7H
2O 0.1-0.5g,
Low grade rock phosphate, the 200-300 order, 5-15g,
Distilled water 1000mL;
Describedly have a liking for the ferrous thiobacillus secondary medium of acid oxidase component:
(NH
4)
2SO
4 1-5g,
KCl 0.1-0.3g,
K
2HPO
4 0.1-1.0g,
MgSO
4·7H
2O 0.1-1.0g,
Ca(NO
3)
2 0.01-0.5g,
Pyritic ashes, the 200-300 order, 10-30g,
Low grade rock phosphate, the 200-300 order, 5-15g,
Distilled water 1000mL;
3) by following mass percent proportioning:
Paddy rice, wheat or maize straw 20-30%,
Ammonium sulfate 5-10%,
Pyritic ashes, the 200-300 order, 10-20%,
Low grade rock phosphate, the 200-300 order, 10-20%,
The secondary bacterium liquid 15-20% of Crius candiyeast,
Have a liking for the secondary bacterium liquid 15-20% of the ferrous thiobacillus of acid oxidase;
Mix after choosing raw material, regulating pH with volumetric concentration 10-20% sulfuric acid is 2.5-3.0, is transferred to fermentor tank and cultivates, and leavening temperature 28-32 ℃, the air flow in the fermentor tank is by feeding with fermentation volume 1: 1-1.5, and tank pressure is 0.5-1kg/cm
2, stirring velocity is 160-180 rev/min, ferments after 4-6 days, packing is made complex microorganism phosphate fertilizer.
2. a kind of method of utilizing low grade rock phosphate to produce complex microorganism phosphate fertilizer according to claim 1, it is characterized in that: the mass content of the Vanadium Pentoxide in FLAKES in the described low grade rock phosphate is lower than 20%.
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CN101905983A (en) * | 2010-07-02 | 2010-12-08 | 云南华卿亿安生物工程有限公司 | Method for treating low-grade and medium-grade phosphate rock with microbial strain |
CN102173878A (en) * | 2011-03-08 | 2011-09-07 | 武汉工程大学 | Method for dissolving medium/low grade phosphate ore powder by utilizing acidophilic heterotroph and acidophilic autotroph |
CN104671861B (en) * | 2015-02-11 | 2017-12-12 | 上海杏王生态科技有限公司 | A kind of organic phosphatic fertiliser and preparation method thereof |
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CN107780278B (en) * | 2017-11-17 | 2020-06-09 | 苏州博进生物技术有限公司 | Microbial treatment method for waste chips generated by setting off fireworks and crackers during spring festival |
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