CN101131362A - Method for fast filtering antibacterial medicine residue in milk-like liquid sample - Google Patents
Method for fast filtering antibacterial medicine residue in milk-like liquid sample Download PDFInfo
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- CN101131362A CN101131362A CNA2007100466073A CN200710046607A CN101131362A CN 101131362 A CN101131362 A CN 101131362A CN A2007100466073 A CNA2007100466073 A CN A2007100466073A CN 200710046607 A CN200710046607 A CN 200710046607A CN 101131362 A CN101131362 A CN 101131362A
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Abstract
The invention discloses a method for rapid screening the residues of antibiotics in milk-like liquid samples, including: preparation of bacillus bacteria culture used in detection, preparation of agar medium detector tube containing working bacteria and indicator, preparation of liquid culture, with the principle that antimicrobial agents can inhibit growth or propagation of bacterial sensitive to it, aspirate the liquid sample with quantitative pipet and drop in to the agar medium detector tube and add the liquid medium used for detection, in a certain temperature conditions culture vertically for a certain period of time, according to the nature and extent of color changes of solid agar medium in the tube to determine whether samples contain residues of antibiotics. Compared with the existing technology, the invention has characteristics of quick and easy, small interference, wide detection spectrum, high sensitivity, high accuracy, and good stability, applicable for high-flux and rapid detection of antibiotics residues in milk-like liquid agricultural products.
Description
Technical field
The present invention relates to antibacterial medicine residue check and analysis technical field, belong to specifically that a kind of growth that utilizes bacterium is subjected to that antibacterials residual in the test sample suppress and the method for antibacterial medicine residue in the rapid screening milk-like liquid sample.
Background technology
Antibacterial medicine residue is the serious problems of puzzlement food security always, various infectious diseases of milk cattle cultivating field widespread use antibacterial drug therapy thereby cause medicament residue in the milk easily.The method that is used for detecting the milk antibacterials now mainly contains high performance liquid chromatography, liquid phase-mass spectrometry analytic approach, gas phase-mass spectrometry analytic approach, thin-layered chromatography isochromatic spectrum method.The major defect that these chromatographic processes exist is: need professional instrument and equipment; The sample pretreatment program is loaded down with trivial details; Complicated operating process; Consuming time tediously long; Need the technician of professional training to operate; Disturbing factor is many; The analytical instrument costliness; Be difficult to popularize in basic unit, generally only be applicable to the laboratory detection; The testing cost height.Immunological detection also is used for the residue detection of antibacterials at milk, but a kind of kit generally can only detect a kind of antibacterials, be subject to disturb and go out false positive, consumables cost height.
Microbial method detect antibacterial medicine residue be according to antibacterials to the physiological function of microorganism, the inhibiting effect of metabolism, come qualitative or determine that quantitatively the sample Chinese traditional medicine is residual, have fast, advantages such as sensitivity, cheapness.At present, the antibacterial medicine residue microorganism detection method of liquid-like such as milk mainly contains triphenyl tetrazolium chloride method, and lists national standard in.But in the specific operation process of this method, adding the quantity that detects the streptococcus thermophilus in the milk can be very big to the testing result influence, can produce false negative or false positive results, and its detection time of while is also longer, meddlesome effort.Therefore, exploitation is cheap, susceptibility is high, can fast detecting milk etc. fluid samples, and the layman can operate, the microbial method residue detection screening technique of credible result becomes urgent problem.
Summary of the invention
The objective of the invention is to overcome the above-mentioned defective that prior art exists in promoting the use of process, a kind of specific large-sized analytic instrument equipment that do not need is provided, has highly sensitive, with a high credibility, the simple screening technique of using method, make it can effectively reduce the detection cost of antibacterial medicine residue in the fluid samples such as milk, alleviate the burden that needs to detect unit, be suitable for basic unit and promote.
Purpose of the present invention is achieved through the following technical solutions:
The method of antibacterial medicine residue in a kind of rapid screening milk-like liquid sample, characteristics are: it comprises that preparation detects agar medium detector tube and the screening that contains bacterial spore and acid base indicator with fluid nutrient medium, preparation, and its concrete steps are as follows:
(1), preparation detects and uses fluid nutrient medium
Get 0.6%～1.5% peptone, 0.4%～1.0% tryptone, 0.15%～0.4% beef extract, 0.4%～0.8% glucose, 0%～1.0% yeast soaks height, 1.0%～2.0% sodium chloride, 1.0%～2.0% milk powder, adding deionized water, to supply cumulative volume be 100%, the aforementioned solution of every 1000mL adds and contains 0.5% lime chloride behind the mixing, 0.25% sodium chloride, inorganic salt solution 20～the 40mL of 0.25% potassium chloride, methoxybenzyl aminopyrimidine solution 30～60mL of 6 μ g/mL, transferring pH behind the mixing is 6.8～7.6,115 ℃ of sterilizations 30 minutes, the cooling back was standby 0～4 ℃ of preservation.
(2), preparation contains the agar medium detector tube of bacterial spore and acid base indicator
A, making gemma rate reach the sporeformer liquid more than 85%
Bacillus is containing 1% tryptone, 0.5% yeast extract, 0.2%K
2HPO
4, cultivate 18～20h for 55 ℃ in the fluid nutrient medium of pH 7.2, transferred species is in the MnCl that contains 0.2～0.3% beef extract, 0.4～0.5% peptone, 0.3～0.4% yeast extract, 50～200mg/L then
2.4H
2O, 2% agar, the solid medium of pH7.2 was cultivated 3～7 days for 50～60 ℃; The spore staining microscopy, when the gemma rate 85% when above, washed and place 90 ℃ of water-bath heat inactivations 20 minutes with 4 ℃ of aseptic deionized waters; Centrifugal 20 minutes of 5000rpm, abandoning supernatant, the sterilization deionized water is floating again, the repeated centrifugation washed twice.Last precipitum is floating with 4 ℃ of aseptic deionized waters, gets sporeformer liquid after the count of bacteria.
B, acid base indicator mix with agar, add deionized water, make acid base indicator and agar concentration reach 50～100 μ g/mL and 1.5～2% respectively, and mixing is transferred pH7.0～7.2, and 115 ℃ were sterilized 30 minutes.
When making agar be cooled to 55～75 ℃ after c, sterilization finish, the sporeformer liquid that rapid adding has been counted makes gemma content reach 1 * 10
6Cfu/mL～1 * 10
8Cfu/mL, abundant immediately mixing also is sub-packed in the sterile tube of vertical state; Every arm adds 50～400 μ L agar, makes the interior agar thickness of pipe reach 0.2～0.6 centimetre, vertically sealing and standby in 0～4 ℃ of refrigeration after the state natural coagulation.
(3), screening
With transfer pipet I 50～200 μ L milk-like liquid samples are joined the agar medium detector tube that contains bacterial spore and acid base indicator, add 50～200 μ L detection fluid nutrient medium with transfer pipet II to the agar medium detector tube again, after vertically placing 55 ℃～70 ℃ to cultivate 1.5～4 hours the agar medium detector tube, filter out the sample that contains antibacterial medicine residue according to the character and the degree of solid agar medium change color in the agar medium detector tube.
Described sporeformer liquid is bacillus stearothermophilus or bacillus subtilis spore bacterium liquid.
Described acid base indicator is bromcresol purple, coeruleum bromocresolis or bromine cresol red indicator.
Described sterile tube is that internal diameter is 0.3～1.5 centimetre, highly is that 1.0～3.0 centimetres transparent column shape polystyrene plastics pipe or volume is the transparent conical centrifuge tube with cover of 0.5～1.0mL.
Detection principle of the present invention is: when the material that does not have inhibition bacterial spore growths such as antibacterials exists, gemma is in prescribing adequate nutrition, can ramp breed under the conditions such as suitable temperature, humidity, and then make nutrient culture media become acidity, this phenomenon usable acid neutralization indicator colour developing indication (being yellow); When residual when antibacterials are arranged in the fluid samples such as milk, the growth and the breeding of gemma are suppressed, though gemma is in prescribing adequate nutrition, but can not ramp breed under the conditions such as suitable temperature, humidity, and then nutrient culture media can not become acidity yet, acid base indicator can not change color (being purple) yet, thereby can detect in the fluid samples such as milk whether antibacterials are arranged, and the degree of depth of the change color of indicator and the concentration of antibacterials are certain reverse correlativity after cultivating, purple is dark more, and antibacterial medicine residue concentration is high more.The present invention to the residual sensitivity of penicillin in the plain chocolate between 2～3ng/mL, consuming time in 3 hours.
The present invention has following characteristics: 1, main agents all provides with the working fluid form, and the method for inspection is convenient and easy; 2, low to supplemental equipment requirement, be particularly suitable for basic unit's uses such as milking station; 3, wide to antibiotic drug test spectrum, the specificity height, highly sensitive, accuracy good, detects the residual sensitivity of penicillin in the plain chocolate between 2～3ng/mL, is lower than national maximum residue limit(MRL) standard, meets the residue detection requirement; 4, sample needs pre-treatment hardly, and tissues such as muscle, liver can be got tissue fluid by modes such as squeezings and detect; 5, can in 2～4 hours, finish detection, fast, save time; 6, the positive test result can be preserved 1.5～3 hours or longer at 55 ℃～70 ℃, preserved more than 30 days at 4 ℃.To provide powerful guarantee for the monitoring of antibacterial medicine residue in the fluid samples such as milk.
Embodiment
Below in conjunction with specific embodiment, further illustrate the present invention.
The screening of embodiment 1 milk sample
(1), preparation detects and uses fluid nutrient medium
Get 1.0% peptone, 0.7% tryptone, 0.2% beef extract, 0.7% glucose, 1.5% sodium chloride, 1.5% milk powder, adding deionized water, to supply cumulative volume be 100mL, add the inorganic salt solution 2.5mL that contains 0.5% lime chloride, 0.25% sodium chloride, 0.25% potassium chloride behind the mixing again, the methoxybenzyl aminopyrimidine solution 4mL of 6 μ g/mL, transferring pH behind the mixing is 7.2,115 ℃ of sterilizations 30 minutes, the cooling back was standby 0～4 ℃ of preservation.
(2), preparation contains the agar medium detector tube of bacterial spore and acid base indicator
A, making gemma rate reach the sporeformer liquid more than 85%
Bacillus alcalophilus is being contained 1% tryptone, 0.5% yeast extract, 0.2%K
2HPO
4, cultivate 18h for 55 ℃ in the fluid nutrient medium of pH 7.2, transferred species is in the MnCl that contains 0.3% beef extract, 0.5% peptone, 0.4% yeast extract, 200mg/L then
2.4H
2O, 2% agar, the solid medium of pH7.2 was cultivated 5 days for 55 ℃.The spore staining microscopy, when the gemma rate 85% when above, washed and place 90 ℃ of water-bath heat inactivations 20 minutes with 4 ℃ of aseptic deionized waters.Centrifugal 20 minutes of 5000rpm, abandoning supernatant, the sterilization deionized water is floating again, the repeated centrifugation washed twice.Last precipitum is floating with 4 ℃ of aseptic deionized waters, obtains bacillus stearothermophilus sporeformer liquid after the count of bacteria.
B, bromcresol purple is mixed with agar, add deionized water 100mL, make bromcresol purple and agar concentration reach 80 μ g/mL and 1.5% respectively, mixing is transferred pH7.0, and 115 ℃ of sterilizations 30 minutes.
Add the gemma liquid of having counted when making agar be cooled to 65 ℃ after c, sterilization finish, make gemma content reach 1 * 10
7About cfu/mL, abundant mixing and to be sub-packed in vertical internal diameter be that 0.8 centimetre, height are 1.5 centimetres transparent column shape polystyrene plastics pipe immediately.Every arm adds 150 μ L agar, makes the interior agar thickness of pipe reach 0.3 centimetre, natural coagulation, and sealing is also standby in 0～4 ℃ of refrigeration.
(3), the screening of milk sample
All reagent should be kept at 0 ℃～4 ℃ before the screening, should allow its room temperature of rising again naturally during use, should in time be kept at 0 ℃～4 ℃ after finishing using.Simultaneously, 64 ± 1 ℃ should be set and arrive to the thermostat of screening usefulness in advance.
The 1st step: water-bath or other thermostat such as sand-bath pot are set at 64 ± 1 ℃;
The 2nd step: get a detector tube, milk sample is shaken up, add in the detector tube with transfer pipet I suck up milk to assigned scale (100 μ L);
The 3rd step: will detect and shake up, and draw with transfer pipet II and detect with fluid nutrient medium to assigned scale (50 μ L), in the adding detector tube with fluid nutrient medium;
The 4th step: other gets two detector tubes, repeats application of sample (a general milk sample uses three detector tubes) by the 2nd, 3 steps respectively;
The 5th step: immediately detector tube is vertically put into 64 ± 1 ℃ of water-baths (attention can not allow detector tube topple) and cultivated and pick up counting;
The 6th step: cultivate and take out detector tube immediately after 2.5 hours, and contrast with the colorimetric card.It is negative all to become yellow decidable as if nutrient culture media color in three detector tubes; If three interior nutrient culture media colors of detector tubes all become whole purples and are judged to be the positive; If wherein in two detector tubes the nutrient culture media color all to become yellow decidable negative; If wherein in two detector tubes the nutrient culture media color all to become the purple decidable positive.
The 7th step: the record result also deals carefully with detector tube
Also can replace milk as negative control deionized water in screening, the penicillin solution that will contain 4ng/mL be as positive control.
The screening of embodiment 2 other samples
Tissues such as meat, liver, kidney can be transferred pH to 7.0 through pressing extracting juice after the weighing and adding an amount of phosphate buffer; Feed, samples such as serum, honey, urine if necessary to different animal samples, add different damping fluids, with control pH.Other operation steps is with embodiment 1.
Claims (5)
1. the method for antibacterial medicine residue in the rapid screening milk-like liquid sample, it is characterized in that it comprises: preparation detects with fluid nutrient medium, prepares the agar medium detector tube and the screening that contain bacterial spore and acid base indicator, and its concrete steps are as follows:
(1), preparation detects and uses fluid nutrient medium
Get 0.6%～1.5% peptone, 0.4%～1.0% tryptone, 0.15%～0.4% beef extract, 0.4%～0.8% glucose, 0%～1.0% yeast extract, 1.0%～2.0% sodium chloride, 1.0%～2.0% milk powder, add deionized water, supplying cumulative volume is 100%; The aforementioned solution of every 1000mL adds the inorganic salt solution 20～40mL that contains 0.5% lime chloride, 0.25% sodium chloride, 0.25% potassium chloride again behind the mixing, methoxybenzyl aminopyrimidine solution 30～60mL of 6 μ g/mL; Transferring pH behind the mixing once more is 6.8～7.6, and 115 ℃ of sterilizations 30 minutes, the cooling back was standby 0～4 ℃ of preservation;
(2), preparation contains the agar medium detector tube of bacterial spore and acid base indicator
A, making gemma rate reach the sporeformer liquid more than 85%;
B, acid base indicator is mixed with agar, add deionized water, make acid base indicator and agar concentration reach 50～100 μ g/mL and 1.5～2% respectively, mixing is transferred pH7.0～7.2, and 115 ℃ of sterilizations 30 minutes;
After c, sterilization finish, when still being in melting state, the b step make it be cooled to 55～75 ℃, and add the sporeformer liquid of step a rapidly, fully be sub-packed in immediately in the sterile tube that is in vertical state behind the mixing, every arm adds 50～400 μ L agar, make the interior agar thickness of pipe reach 0.2～0.6 centimetre, vertically sealing and standby after the state natural coagulation in 0～4 ℃ of refrigeration;
(3), screening
With transfer pipet I 50～200 μ L milk-like liquid samples are joined the agar medium detector tube that contains bacterial spore and acid base indicator, add 50～200 μ L detection fluid nutrient medium with transfer pipet II to the agar medium detector tube again, after vertically placing 55 ℃～70 ℃ to cultivate 1.5～4 hours the agar medium detector tube, filter out the sample that contains antibacterial medicine residue according to the character and the degree of solid agar medium change color in the agar medium detector tube.
2. method according to claim 1 is characterized in that described sporeformer liquid is bacillus stearothermophilus or bacillus subtilis spore bacterium liquid.
3. method according to claim 1 is characterized in that described acid base indicator is bromcresol purple, coeruleum bromocresolis or bromine cresol red indicator.
4. method according to claim 1 is characterized in that described sterile tube is that internal diameter is 0.3～1.5 centimetre, highly is that 1.0～3.0 centimetres transparent column shape polystyrene plastics pipe or volume is the transparent conical centrifuge tube with cover of 0.5～1.0mL.
5. method according to claim 1 is characterized in that described gemma bacterial concentration is 1 * 10
6Cfu/mL～1 * 10
8Cfu/mL.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101281138B (en) * | 2008-03-04 | 2010-06-09 | 浙江大学 | Method for quickly detecting whether containing fermentation inhibitor or not in dairy food |
| CN103411967A (en) * | 2013-08-20 | 2013-11-27 | 安徽华恒生物工程有限公司 | Detection plate for activity of L-aspartic acid-beta-decarboxylase and fabrication methods thereof |
| CN105675604A (en) * | 2016-03-31 | 2016-06-15 | 山东五洲检测有限公司 | Method for detecting antibiotic residues in milk |
| CN106554987A (en) * | 2015-09-29 | 2017-04-05 | 北京华益精点生物技术有限公司 | The test kit and its detection method of antibiotic in a kind of detection food-borne animal tissue |
| CN106868094A (en) * | 2017-02-22 | 2017-06-20 | 湖南亚华乳业有限公司 | The method for quick of antibiotic residue in a kind of raw milk |
| CN107860769A (en) * | 2017-11-08 | 2018-03-30 | 新疆天润生物科技股份有限公司 | A kind of quick determination method, its detection preparation and application for being used to prevent and treat milk beer production pickup dye |
| CN109536568A (en) * | 2018-11-15 | 2019-03-29 | 华南农业大学 | A kind of quick detection produces method, kit and its application of carbapenem enzyme bacterial strain |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| NL7806086A (en) * | 1978-06-05 | 1979-12-07 | Gist Brocades Nv | ANALYSIS UNIT. |
| AU2584588A (en) * | 1987-12-01 | 1989-06-01 | Abbott Laboratories | Antibiotic resistance testing assay |
| CZ251094A3 (en) * | 1993-02-11 | 1995-02-15 | Gist Brocades Nv | Unit for detecting residues of antibacterial substances in liquids |
| CN1327229C (en) * | 2004-05-10 | 2007-07-18 | 黑龙江省完达山乳业股份有限公司 | Method for detecting antibiotic in milk through PH meter |
| CN1259430C (en) * | 2004-06-07 | 2006-06-14 | 天津科技大学 | Microbiological preparation preparing method for detecting antibiotics residual weight in cow's milk and use thereof |
| ES2263361B1 (en) * | 2004-11-30 | 2007-11-01 | Universidad De Zaragoza | METHOD OF DETECTION OF ANTIBIOTIC RESIDUES AND OTHER ANTIBACTERIAL COMPOUNDS. |
| CN1793922A (en) * | 2005-12-15 | 2006-06-28 | 丁东新 | Fresh milk antibiotics residue detection box and its manufacturing method |
-
2007
- 2007-09-29 CN CNB2007100466073A patent/CN100554950C/en not_active Expired - Fee Related
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101281138B (en) * | 2008-03-04 | 2010-06-09 | 浙江大学 | Method for quickly detecting whether containing fermentation inhibitor or not in dairy food |
| CN103411967A (en) * | 2013-08-20 | 2013-11-27 | 安徽华恒生物工程有限公司 | Detection plate for activity of L-aspartic acid-beta-decarboxylase and fabrication methods thereof |
| CN106554987A (en) * | 2015-09-29 | 2017-04-05 | 北京华益精点生物技术有限公司 | The test kit and its detection method of antibiotic in a kind of detection food-borne animal tissue |
| CN105675604A (en) * | 2016-03-31 | 2016-06-15 | 山东五洲检测有限公司 | Method for detecting antibiotic residues in milk |
| CN106868094A (en) * | 2017-02-22 | 2017-06-20 | 湖南亚华乳业有限公司 | The method for quick of antibiotic residue in a kind of raw milk |
| CN107860769A (en) * | 2017-11-08 | 2018-03-30 | 新疆天润生物科技股份有限公司 | A kind of quick determination method, its detection preparation and application for being used to prevent and treat milk beer production pickup dye |
| CN109536568A (en) * | 2018-11-15 | 2019-03-29 | 华南农业大学 | A kind of quick detection produces method, kit and its application of carbapenem enzyme bacterial strain |
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