CL2013003190A1 - Medio de cultivo para celulas mesenquimales humanas. - Google Patents

Medio de cultivo para celulas mesenquimales humanas.

Info

Publication number
CL2013003190A1
CL2013003190A1 CL2013003190A CL2013003190A CL2013003190A1 CL 2013003190 A1 CL2013003190 A1 CL 2013003190A1 CL 2013003190 A CL2013003190 A CL 2013003190A CL 2013003190 A CL2013003190 A CL 2013003190A CL 2013003190 A1 CL2013003190 A1 CL 2013003190A1
Authority
CL
Chile
Prior art keywords
culture medium
mesenchymal cells
human mesenchymal
grow
present
Prior art date
Application number
CL2013003190A
Other languages
English (en)
Inventor
Cervantes Jose Maria Diez
Rodriguez Rodrigo Gajardo
Original Assignee
Grifols Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Grifols Sa filed Critical Grifols Sa
Publication of CL2013003190A1 publication Critical patent/CL2013003190A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0668—Mesenchymal stem cells from other natural sources
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0607—Non-embryonic pluripotent stem cells, e.g. MASC
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0663—Bone marrow mesenchymal stem cells (BM-MSC)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/33—Insulin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/11—Coculture with; Conditioned medium produced by blood or immune system cells
    • C12N2502/115—Platelets, megakaryocytes

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Chemical & Material Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Rheumatology (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

LA PRESENTE INVENCIÓN SE REFIERE A UN NUEVO MEDIO DE CULTIVO DE CÉLULAS MADRE MESENQUIMALES HUMANAS (HMSC). MÁS EN PARTICULAR, LA PRESENTE INVENCIÓN SE REFIERE A UN MEDIO DE CULTIVO EN EL QUE SON CAPACES DE CRECER LÍNEAS DE HMSC, INCLUSO LAS QUE NO CRECEN EN MEDIOS DE CULTIVOS HABITUALMENTE UTILIZADOS PARA ESTE TIPO DE CÉLULAS.
CL2013003190A 2012-11-13 2013-11-07 Medio de cultivo para celulas mesenquimales humanas. CL2013003190A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES201231753 2012-11-13

Publications (1)

Publication Number Publication Date
CL2013003190A1 true CL2013003190A1 (es) 2014-08-08

Family

ID=47913258

Family Applications (1)

Application Number Title Priority Date Filing Date
CL2013003190A CL2013003190A1 (es) 2012-11-13 2013-11-07 Medio de cultivo para celulas mesenquimales humanas.

Country Status (23)

Country Link
US (1) US8835175B2 (es)
EP (1) EP2730648B1 (es)
JP (1) JP6084548B2 (es)
KR (1) KR101831469B1 (es)
CN (2) CN103805561A (es)
AR (1) AR093443A1 (es)
AU (1) AU2013202314B2 (es)
BR (1) BR102013027139A2 (es)
CA (1) CA2830266C (es)
CL (1) CL2013003190A1 (es)
ES (1) ES2591205T3 (es)
HK (1) HK1195100A1 (es)
HU (1) HUE028991T2 (es)
IL (1) IL229203A (es)
MX (1) MX2013012128A (es)
MY (1) MY161163A (es)
PL (1) PL2730648T3 (es)
PT (1) PT2730648T (es)
RU (1) RU2640556C2 (es)
SG (1) SG2013075874A (es)
TW (1) TWI609963B (es)
UY (1) UY35104A (es)
ZA (1) ZA201307857B (es)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8835175B2 (en) * 2012-11-13 2014-09-16 Grifols, S.A. Culture medium for human mesenchymal stem cells
US10513689B2 (en) 2016-04-29 2019-12-24 Hope Biosciences, Llc Culture media for multipotent stem cells
TWI774111B (zh) * 2019-11-04 2022-08-11 胡翊威 一種製備單核球衍生的訊息細胞混合物的方法及其用途

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH07184634A (ja) * 1993-12-28 1995-07-25 Ajinomoto Co Inc 微生物好気培養における培養方法及び装置
JP2004504272A (ja) * 2000-03-23 2004-02-12 グリーンビル　ホスピタル　システム 双機能癌治療剤
ZA200404101B (en) * 2003-05-26 2005-03-07 Reliance Life Sciences Pvt Ltd In vitro culture of Mesenchymal Stem Cells (MSC) and a process for the preparation thereof for therapeutic use.
WO2005085422A1 (en) * 2004-02-27 2005-09-15 Michigan State University Adult stem cells and uses thereof
JP4745750B2 (ja) * 2005-08-01 2011-08-10 株式会社ツーセル 動物幹細胞培養用無血清培地
US7989205B2 (en) 2005-10-06 2011-08-02 American Cryostem Corporation Cell culture media, kits and methods of use
BRPI0713572A2 (pt) * 2006-06-20 2012-10-23 Stephen Duguay meios livres de soro e seus usos para expansão de condrócitos
US9095562B2 (en) * 2007-07-05 2015-08-04 Regenerative Sciences, Inc. Methods and compositions for optimized expansion and implantation of mesenchymal stem cells
CN101412985B (zh) * 2007-10-15 2012-06-13 华东理工大学 用于骨髓间充质干细胞体外培养和扩增的无血清培养基
CN101918542B (zh) * 2007-12-28 2013-06-12 富士瑞必欧株式会社 哺乳动物体细胞用培养基以及该培养基的添加剂
ES2360782B1 (es) * 2009-07-28 2012-03-12 Grifols, S.A. Medios para cultivo de células de mamíferos que comprenden sobrenadante de etapas del fraccionamiento de Cohn y uso de los mismos.
US9394521B2 (en) 2010-03-10 2016-07-19 Two Cells Co., Ltd. Cell preparation containing mesenchymal stem cells, and method for producing same
CN101984048A (zh) * 2010-11-24 2011-03-09 中国人民解放军军事医学科学院放射与辐射医学研究所 一种培养间充质干细胞的培养基
US8835175B2 (en) * 2012-11-13 2014-09-16 Grifols, S.A. Culture medium for human mesenchymal stem cells
US9321995B2 (en) * 2012-12-20 2016-04-26 Suzhou Biowisetech Co., Ltd. Stem cell culture medium and its applications as well as a stem cell culture method

Also Published As

Publication number Publication date
RU2013145425A (ru) 2015-04-20
AR093443A1 (es) 2015-06-10
KR101831469B1 (ko) 2018-02-22
HUE028991T2 (en) 2017-01-30
CA2830266C (en) 2018-09-18
PL2730648T3 (pl) 2016-12-30
CN111154717A (zh) 2020-05-15
HK1195100A1 (zh) 2014-10-31
JP6084548B2 (ja) 2017-02-22
MX2013012128A (es) 2014-05-22
US8835175B2 (en) 2014-09-16
MY161163A (en) 2017-04-14
US20140134724A1 (en) 2014-05-15
TWI609963B (zh) 2018-01-01
PT2730648T (pt) 2016-07-28
AU2013202314A1 (en) 2014-05-29
IL229203A (en) 2016-03-31
JP2014097055A (ja) 2014-05-29
ES2591205T3 (es) 2016-11-25
AU2013202314B2 (en) 2014-11-13
KR20140061263A (ko) 2014-05-21
NZ616977A (en) 2015-05-29
EP2730648A1 (en) 2014-05-14
RU2640556C2 (ru) 2018-01-09
EP2730648B1 (en) 2016-06-15
ZA201307857B (en) 2015-11-25
TW201418465A (zh) 2014-05-16
UY35104A (es) 2014-02-28
CN103805561A (zh) 2014-05-21
CA2830266A1 (en) 2014-05-13
SG2013075874A (en) 2014-06-27
BR102013027139A2 (pt) 2015-06-30

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