CA2252524A1 - Lignees de cellules souches embryonnaires de lapin multipotentes et leur utilisation pour produire des lapins chimeriques - Google Patents
Lignees de cellules souches embryonnaires de lapin multipotentes et leur utilisation pour produire des lapins chimeriques Download PDFInfo
- Publication number
- CA2252524A1 CA2252524A1 CA002252524A CA2252524A CA2252524A1 CA 2252524 A1 CA2252524 A1 CA 2252524A1 CA 002252524 A CA002252524 A CA 002252524A CA 2252524 A CA2252524 A CA 2252524A CA 2252524 A1 CA2252524 A1 CA 2252524A1
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- Prior art keywords
- cells
- rabbit
- embryonic stem
- medium
- cell lines
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/873—Techniques for producing new embryos, e.g. nuclear transfer, manipulation of totipotent cells or production of chimeric embryos
- C12N15/877—Techniques for producing new mammalian cloned embryos
- C12N15/8777—Rabbit embryos
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0603—Embryonic cells ; Embryoid bodies
- C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/20—Cytokines; Chemokines
- C12N2501/23—Interleukins [IL]
- C12N2501/235—Leukemia inhibitory factor [LIF]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/30—Hormones
- C12N2501/33—Insulin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2502/00—Coculture with; Conditioned medium produced by
- C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2509/00—Methods for the dissociation of cells, e.g. specific use of enzymes
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Developmental Biology & Embryology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Gynecology & Obstetrics (AREA)
- Reproductive Health (AREA)
- Plant Pathology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Cell Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Lignée de cellules souches embryonnaires de lapin, qui comprend au moins 70 %, de préférence 80 à 90 %, de cellules indifférenciées et obtenues par isolement de l'amas cellulaire interne de blastocystes 5,5 jours postcoïtus et par culture desdites lignées sur des cellules nourricières dans un milieu pour cellules souches embryonnaires de lapin. La présente invention concerne en outre l'optimisation de la dérivation et de la conservation de ladite lignée cellulaire et l'utilisation de cette dernière entre autres pour produire des lapins chimériques.
Applications Claiming Priority (6)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP96201169 | 1996-04-29 | ||
EP96201169.8 | 1996-04-29 | ||
EP96203060 | 1996-11-04 | ||
EP96203060.7 | 1996-11-04 | ||
EP97200168.9 | 1997-01-22 | ||
EP97200168 | 1997-01-22 |
Publications (1)
Publication Number | Publication Date |
---|---|
CA2252524A1 true CA2252524A1 (fr) | 1997-11-06 |
Family
ID=27237546
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA002252524A Abandoned CA2252524A1 (fr) | 1996-04-29 | 1997-04-29 | Lignees de cellules souches embryonnaires de lapin multipotentes et leur utilisation pour produire des lapins chimeriques |
Country Status (6)
Country | Link |
---|---|
EP (1) | EP0907722A1 (fr) |
JP (1) | JP2000508919A (fr) |
CN (1) | CN1217746A (fr) |
AU (1) | AU2892997A (fr) |
CA (1) | CA2252524A1 (fr) |
WO (1) | WO1997041209A1 (fr) |
Families Citing this family (25)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0941310A1 (fr) | 1996-10-11 | 1999-09-15 | THE TEXAS A&M UNIVERSITY SYSTEM | Techniques permettant la production de cellules sexuelles primordiales et d'especes animales transgeniques |
JP2002527036A (ja) * | 1997-11-25 | 2002-08-27 | エイアールシー ジェノミック リサーチ | 多能性胚性幹細胞および多能性胚性幹細胞を得る方法 |
WO1999027076A1 (fr) * | 1997-11-25 | 1999-06-03 | Arc Genomic Research | Cellules souches embryonnaires multipotentes et procedes permettant de les obtenir |
US6238922B1 (en) * | 1999-02-26 | 2001-05-29 | Stemcells, Inc. | Use of collagenase in the preparation of neural stem cell cultures |
KR100771294B1 (ko) * | 1999-12-14 | 2007-10-29 | 써모 바이오스타, 인크. | 폴리펩티드 및 항원 안정화 희석제 |
JP4795616B2 (ja) * | 2000-06-20 | 2011-10-19 | イーエス・セル・インターナショナル・プライヴェート・リミテッド | Bmp−2経路アンタゴニストの存在下においてes細胞を培養することによって胚性幹細胞の分化をコントロールする方法 |
EP1217071B1 (fr) | 2000-12-22 | 2010-04-28 | Institut National De La Recherche Agronomique | Expression position-indépendante et tissus-spécifique d' un transgène dans le lait d'animaux transgéniques |
FR2834518B1 (fr) * | 2002-01-10 | 2005-03-18 | Agronomique Inst Nat Rech | Procede de clonage nucleaire chez le lapin et utilisations |
US8846393B2 (en) | 2005-11-29 | 2014-09-30 | Gamida-Cell Ltd. | Methods of improving stem cell homing and engraftment |
EP2508595B1 (fr) | 2009-12-01 | 2016-11-23 | National Cancer Center | Procédé de construction d'un rat chimérique à l'aide de cellules souches embryonnaires de rat |
US20120244619A1 (en) | 2009-12-09 | 2012-09-27 | Kyoto University | Composition for promoting differentiation of pluripotent stem cells into cardiac muscle cells which comprises nitrovin |
CN102933074B (zh) | 2010-06-11 | 2015-04-08 | 瑞泽恩制药公司 | 由xy es细胞制备能育的xy雌性动物 |
US9499790B2 (en) | 2010-08-26 | 2016-11-22 | Kyoto University | Method for promoting differentiation of pluripotent stem cells into cardiac muscle cells |
CN103209968B (zh) | 2010-08-26 | 2015-08-05 | 国立大学法人京都大学 | 多能干细胞心肌细胞分化促进剂 |
KR101996343B1 (ko) | 2012-01-27 | 2019-07-05 | 고쿠리츠 다이가쿠 호진 교토 다이가쿠 | 전능성 줄기세포의 심근분화 유도방법 |
BR112014020119A2 (pt) | 2012-02-13 | 2020-10-27 | Gamida-Cell Ltd | cultura de células-tronco mesenquimais |
US9175266B2 (en) | 2012-07-23 | 2015-11-03 | Gamida Cell Ltd. | Enhancement of natural killer (NK) cell proliferation and activity |
US9567569B2 (en) | 2012-07-23 | 2017-02-14 | Gamida Cell Ltd. | Methods of culturing and expanding mesenchymal stem cells |
EP2966166B1 (fr) | 2013-03-08 | 2019-04-03 | Kyoto University | Promoteur de différenciation d'une cellule souche pluripotente en myocarde, lequel comprend un inhibiteur d'un récepteur d'egf |
WO2015037706A1 (fr) | 2013-09-13 | 2015-03-19 | 国立大学法人京都大学 | Composé favorisant la différenciation de cellules souches pluripotentes en cardiomyocytes |
WO2015182765A1 (fr) | 2014-05-30 | 2015-12-03 | 国立大学法人京都大学 | Procédé d'induction de la différenciation myocardique des cellules souches pluripotentes à l'aide d'un composé de faible poids moléculaire |
HUE041584T2 (hu) | 2014-06-26 | 2019-05-28 | Regeneron Pharma | Célzott genetikai módosítások és alkalmazási módszerek és készítmények |
CA2998642C (fr) | 2015-09-17 | 2023-09-12 | Regeneron Pharmaceuticals, Inc. | Selection de cellules pluripotentes pour la production de souris femelles xy fertiles |
CN109153973A (zh) | 2016-03-18 | 2019-01-04 | 国立大学法人京都大学 | 用于冷冻多能干细胞来源心肌细胞的聚集体的方法 |
CN109609446B (zh) * | 2018-11-14 | 2020-11-03 | 广西大学 | 一种用于分离培养兔胚胎干细胞的培养液和方法 |
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JP2740320B2 (ja) * | 1988-08-04 | 1998-04-15 | アムラド・コーポレイション・リミテッド | 胚幹細胞のインビトロ増殖 |
US5523226A (en) * | 1993-05-14 | 1996-06-04 | Biotechnology Research And Development Corp. | Transgenic swine compositions and methods |
-
1997
- 1997-04-29 CA CA002252524A patent/CA2252524A1/fr not_active Abandoned
- 1997-04-29 JP JP9538604A patent/JP2000508919A/ja active Pending
- 1997-04-29 CN CN97194183A patent/CN1217746A/zh active Pending
- 1997-04-29 EP EP97922992A patent/EP0907722A1/fr not_active Withdrawn
- 1997-04-29 WO PCT/EP1997/002323 patent/WO1997041209A1/fr not_active Application Discontinuation
- 1997-04-29 AU AU28929/97A patent/AU2892997A/en not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
AU2892997A (en) | 1997-11-19 |
JP2000508919A (ja) | 2000-07-18 |
WO1997041209A1 (fr) | 1997-11-06 |
CN1217746A (zh) | 1999-05-26 |
EP0907722A1 (fr) | 1999-04-14 |
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