CA2211018A1 - Seed coat specific dna regulatory region and peroxidase - Google Patents
Seed coat specific dna regulatory region and peroxidaseInfo
- Publication number
- CA2211018A1 CA2211018A1 CA 2211018 CA2211018A CA2211018A1 CA 2211018 A1 CA2211018 A1 CA 2211018A1 CA 2211018 CA2211018 CA 2211018 CA 2211018 A CA2211018 A CA 2211018A CA 2211018 A1 CA2211018 A1 CA 2211018A1
- Authority
- CA
- Canada
- Prior art keywords
- peroxidase
- seed coat
- epep
- proteins
- dna regulatory
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 102000003992 Peroxidases Human genes 0.000 title abstract 8
- 108040007629 peroxidase activity proteins Proteins 0.000 title abstract 8
- 230000001105 regulatory effect Effects 0.000 title abstract 3
- 108090000623 proteins and genes Proteins 0.000 abstract 5
- 108020004414 DNA Proteins 0.000 abstract 3
- 102000004169 proteins and genes Human genes 0.000 abstract 3
- 108700028369 Alleles Proteins 0.000 abstract 2
- 102000054765 polymorphisms of proteins Human genes 0.000 abstract 2
- 241000193388 Bacillus thuringiensis Species 0.000 abstract 1
- 108090000565 Capsid Proteins Proteins 0.000 abstract 1
- 101710172503 Chemokine-binding protein Proteins 0.000 abstract 1
- 241000238631 Hexapoda Species 0.000 abstract 1
- 108010076504 Protein Sorting Signals Proteins 0.000 abstract 1
- 101710138270 PspA protein Proteins 0.000 abstract 1
- 108700005079 Recessive Genes Proteins 0.000 abstract 1
- 102000052708 Recessive Genes Human genes 0.000 abstract 1
- 108091081024 Start codon Proteins 0.000 abstract 1
- 208000036142 Viral infection Diseases 0.000 abstract 1
- 125000003275 alpha amino acid group Chemical group 0.000 abstract 1
- 150000001413 amino acids Chemical class 0.000 abstract 1
- 239000002299 complementary DNA Substances 0.000 abstract 1
- 230000000694 effects Effects 0.000 abstract 1
- 230000002363 herbicidal effect Effects 0.000 abstract 1
- 239000004009 herbicide Substances 0.000 abstract 1
- 230000000050 nutritive effect Effects 0.000 abstract 1
- 244000052769 pathogen Species 0.000 abstract 1
- 239000002243 precursor Substances 0.000 abstract 1
- 239000000523 sample Substances 0.000 abstract 1
- 239000013598 vector Substances 0.000 abstract 1
- 230000009385 viral infection Effects 0.000 abstract 1
Landscapes
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
A novel seed coat specific peroxidase genomic sequence is characterized and presented. Adjacent DNA regulatory regions have also been characterized. The seed coat peroxidase is translated as a 352 amino acid precursor protein of 38 kDa comprising a 26 amino acid signal sequence which when cleaved results in a 35 kDa protein. Plants containing a dominant Ep allele accumulate large amounts of peroxidase in the hourglass cells of the subepidermis. Homozygous recessive epep genotypes do not accumulate peroxidase in the hourglass cells and are much reduced in total seed coat peroxidase activity. Probes derived from the cDNA, or genomic DNA can be used to detect polymorphisms that distinguished EpEp and epep genotypes. Cosegregation of the polymorphisms in an F2 population from a cross of EpEp and epep plants shows that the Ep locus encodes the seed coat peroxidase protein. Comparison of Ep and ep alleles indicates that the recessive gene lacks 87 bp of sequence encompassing the translation start codon. The heterologous expression, as well as vectors and hosts to be used for the expression of the seed coat peroxidase, are also disclosed. The seed-specific DNA regulatory region may be used to control expression of genes of interest such as i) genes encoding herbicide resistance, or ii) biological control of insects or pathogens (e.g. B. thuringiensis), or iii) viral coat proteins to protect against viral infections, or iv) proteins of commercial interest (e.g.
pharmaceutical), and v) proteins that alter the nutritive value, taste, or processing of seeds.
pharmaceutical), and v) proteins that alter the nutritive value, taste, or processing of seeds.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CA 2211018 CA2211018C (en) | 1996-09-30 | 1997-09-19 | Seed coat specific dna regulatory region and peroxidase |
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US72341496A | 1996-09-30 | 1996-09-30 | |
| CA2,186,833 | 1996-09-30 | ||
| CA 2186833 CA2186833A1 (en) | 1996-09-30 | 1996-09-30 | Seed coat dna regulatory region and peroxidase |
| US08/723,414 | 1996-09-30 | ||
| CA 2211018 CA2211018C (en) | 1996-09-30 | 1997-09-19 | Seed coat specific dna regulatory region and peroxidase |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2211018A1 true CA2211018A1 (en) | 1998-03-30 |
| CA2211018C CA2211018C (en) | 2009-03-03 |
Family
ID=27170202
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA 2211018 Expired - Fee Related CA2211018C (en) | 1996-09-30 | 1997-09-19 | Seed coat specific dna regulatory region and peroxidase |
Country Status (1)
| Country | Link |
|---|---|
| CA (1) | CA2211018C (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1999053067A2 (en) * | 1998-04-13 | 1999-10-21 | Her Majesty In Right Of Canada As Represented By The Minister Of Agriculture And Agri-Food Canada | Seed-coat promoters, genes and gene products |
| US6586583B1 (en) * | 1995-10-27 | 2003-07-01 | Indiana Crop Improvement Association | Soybean peroxidase gene family and an assay for detecting soybean peroxidase activity |
| US7396978B2 (en) | 1995-05-15 | 2008-07-08 | Her Majesty The Queen In Right Of Canada, As Represented By The Minister Of Agriculture And Agri-Food | Seed coat gene and gene product |
| CN112707957A (en) * | 2021-02-10 | 2021-04-27 | 中国农业科学院作物科学研究所 | Soybean meristem gene GmWUS2 and application thereof in root nodule development |
-
1997
- 1997-09-19 CA CA 2211018 patent/CA2211018C/en not_active Expired - Fee Related
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7396978B2 (en) | 1995-05-15 | 2008-07-08 | Her Majesty The Queen In Right Of Canada, As Represented By The Minister Of Agriculture And Agri-Food | Seed coat gene and gene product |
| US6586583B1 (en) * | 1995-10-27 | 2003-07-01 | Indiana Crop Improvement Association | Soybean peroxidase gene family and an assay for detecting soybean peroxidase activity |
| WO1999053067A2 (en) * | 1998-04-13 | 1999-10-21 | Her Majesty In Right Of Canada As Represented By The Minister Of Agriculture And Agri-Food Canada | Seed-coat promoters, genes and gene products |
| WO1999053067A3 (en) * | 1998-04-13 | 1999-12-09 | Ca Minister Agriculture & Food | Seed-coat promoters, genes and gene products |
| CN112707957A (en) * | 2021-02-10 | 2021-04-27 | 中国农业科学院作物科学研究所 | Soybean meristem gene GmWUS2 and application thereof in root nodule development |
| CN112707957B (en) * | 2021-02-10 | 2022-07-12 | 中国农业科学院作物科学研究所 | Soybean meristem gene GmWUS2 and application thereof in root nodule development |
Also Published As
| Publication number | Publication date |
|---|---|
| CA2211018C (en) | 2009-03-03 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request | ||
| MKLA | Lapsed |