ATE403863T1 - METHOD FOR DETECTING INTRACELLULAR ATP AND/OR GENE EXPRESSION - Google Patents
METHOD FOR DETECTING INTRACELLULAR ATP AND/OR GENE EXPRESSIONInfo
- Publication number
- ATE403863T1 ATE403863T1 AT02777526T AT02777526T ATE403863T1 AT E403863 T1 ATE403863 T1 AT E403863T1 AT 02777526 T AT02777526 T AT 02777526T AT 02777526 T AT02777526 T AT 02777526T AT E403863 T1 ATE403863 T1 AT E403863T1
- Authority
- AT
- Austria
- Prior art keywords
- gene expression
- intracellular atp
- cell
- detecting intracellular
- luciferin
- Prior art date
Links
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
A method for measuring intracellular ATP and/or gene expression, which method comprises transforming a cell with a construct which encodes a luciferase and a luciferin recycling protein, introducing luciferin into said cell, and monitoring biolominescent signals from said cell. Novel luciferin recycling proteins are also described and these, together with their use in the production of optically active enzyme substrates are claimed.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB0127292A GB0127292D0 (en) | 2001-11-14 | 2001-11-14 | Signal system and elements used therein |
GB0205201A GB0205201D0 (en) | 2001-11-14 | 2002-03-06 | Signal system amd elements used therein |
Publications (1)
Publication Number | Publication Date |
---|---|
ATE403863T1 true ATE403863T1 (en) | 2008-08-15 |
Family
ID=9925737
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AT02777526T ATE403863T1 (en) | 2001-11-14 | 2002-11-13 | METHOD FOR DETECTING INTRACELLULAR ATP AND/OR GENE EXPRESSION |
Country Status (5)
Country | Link |
---|---|
CN (1) | CN101003832A (en) |
AT (1) | ATE403863T1 (en) |
DE (1) | DE60228131D1 (en) |
GB (2) | GB0127292D0 (en) |
ZA (1) | ZA200403508B (en) |
Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US8962854B2 (en) * | 2011-08-24 | 2015-02-24 | The University Of Electro-Communications | Luminescent substrate for luciferase |
CN110577961B (en) * | 2019-09-23 | 2021-05-04 | 安徽师范大学 | Construction method of heat-stable malic acid dehydrogenase gene, encoded protein and application thereof |
CN113186145A (en) * | 2021-05-19 | 2021-07-30 | 山东大学 | Tumor-targeted bacteria with visualization function, visualization method and application thereof |
-
2001
- 2001-11-14 GB GB0127292A patent/GB0127292D0/en not_active Ceased
-
2002
- 2002-03-06 GB GB0205201A patent/GB0205201D0/en not_active Ceased
- 2002-11-13 CN CN 200710003863 patent/CN101003832A/en active Pending
- 2002-11-13 AT AT02777526T patent/ATE403863T1/en not_active IP Right Cessation
- 2002-11-13 DE DE60228131T patent/DE60228131D1/en not_active Expired - Lifetime
-
2004
- 2004-05-07 ZA ZA200403508A patent/ZA200403508B/en unknown
Also Published As
Publication number | Publication date |
---|---|
DE60228131D1 (en) | 2008-09-18 |
ZA200403508B (en) | 2006-12-27 |
GB0205201D0 (en) | 2002-04-17 |
CN101003832A (en) | 2007-07-25 |
GB0127292D0 (en) | 2002-01-02 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |