ATE403863T1 - METHOD FOR DETECTING INTRACELLULAR ATP AND/OR GENE EXPRESSION - Google Patents

METHOD FOR DETECTING INTRACELLULAR ATP AND/OR GENE EXPRESSION

Info

Publication number
ATE403863T1
ATE403863T1 AT02777526T AT02777526T ATE403863T1 AT E403863 T1 ATE403863 T1 AT E403863T1 AT 02777526 T AT02777526 T AT 02777526T AT 02777526 T AT02777526 T AT 02777526T AT E403863 T1 ATE403863 T1 AT E403863T1
Authority
AT
Austria
Prior art keywords
gene expression
intracellular atp
cell
detecting intracellular
luciferin
Prior art date
Application number
AT02777526T
Other languages
German (de)
Inventor
John Day
David Squirrel
Mark Bailey
Peter White
Original Assignee
Secr Defence
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Secr Defence filed Critical Secr Defence
Application granted granted Critical
Publication of ATE403863T1 publication Critical patent/ATE403863T1/en

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Landscapes

  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

A method for measuring intracellular ATP and/or gene expression, which method comprises transforming a cell with a construct which encodes a luciferase and a luciferin recycling protein, introducing luciferin into said cell, and monitoring biolominescent signals from said cell. Novel luciferin recycling proteins are also described and these, together with their use in the production of optically active enzyme substrates are claimed.
AT02777526T 2001-11-14 2002-11-13 METHOD FOR DETECTING INTRACELLULAR ATP AND/OR GENE EXPRESSION ATE403863T1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB0127292A GB0127292D0 (en) 2001-11-14 2001-11-14 Signal system and elements used therein
GB0205201A GB0205201D0 (en) 2001-11-14 2002-03-06 Signal system amd elements used therein

Publications (1)

Publication Number Publication Date
ATE403863T1 true ATE403863T1 (en) 2008-08-15

Family

ID=9925737

Family Applications (1)

Application Number Title Priority Date Filing Date
AT02777526T ATE403863T1 (en) 2001-11-14 2002-11-13 METHOD FOR DETECTING INTRACELLULAR ATP AND/OR GENE EXPRESSION

Country Status (5)

Country Link
CN (1) CN101003832A (en)
AT (1) ATE403863T1 (en)
DE (1) DE60228131D1 (en)
GB (2) GB0127292D0 (en)
ZA (1) ZA200403508B (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8962854B2 (en) * 2011-08-24 2015-02-24 The University Of Electro-Communications Luminescent substrate for luciferase
CN110577961B (en) * 2019-09-23 2021-05-04 安徽师范大学 Construction method of heat-stable malic acid dehydrogenase gene, encoded protein and application thereof
CN113186145A (en) * 2021-05-19 2021-07-30 山东大学 Tumor-targeted bacteria with visualization function, visualization method and application thereof

Also Published As

Publication number Publication date
DE60228131D1 (en) 2008-09-18
ZA200403508B (en) 2006-12-27
GB0205201D0 (en) 2002-04-17
CN101003832A (en) 2007-07-25
GB0127292D0 (en) 2002-01-02

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