AR034140A1 - Procedimiento para transformar celulas vegetales, por la introduccion en dichas celulas de un gen heterologo, con un gen de tolerancia a los inhibidores de hppd, como marcador de seleccion y procedimiento de obtencion de plantas transgenicas que comprenden dicho gen heterologo integrado en su genoma - Google Patents

Procedimiento para transformar celulas vegetales, por la introduccion en dichas celulas de un gen heterologo, con un gen de tolerancia a los inhibidores de hppd, como marcador de seleccion y procedimiento de obtencion de plantas transgenicas que comprenden dicho gen heterologo integrado en su genoma

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Publication number
AR034140A1
AR034140A1 ARP010103848A ARP010103848A AR034140A1 AR 034140 A1 AR034140 A1 AR 034140A1 AR P010103848 A ARP010103848 A AR P010103848A AR P010103848 A ARP010103848 A AR P010103848A AR 034140 A1 AR034140 A1 AR 034140A1
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AR
Argentina
Prior art keywords
gene
cells
procedure
selection marker
competent
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Application number
ARP010103848A
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English (en)
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Aventis Cropscience Sa
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Publication date
Application filed by Aventis Cropscience Sa filed Critical Aventis Cropscience Sa
Publication of AR034140A1 publication Critical patent/AR034140A1/es

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

Se describe y reivindica un procedimiento de transformación de células vegetales por introducción de un gen heterólogo en dichas células vegetales con un gen de tolerancia a los inhibidores de HPPD como marcador de selección, comprendiendo dicho procedimiento las etapas de: a) preparación y cultivo de células vegetales competentes destinadas a recibir el gen heterólogo en un medio apropiado; b) transformación de las células competentes con el gen heterólogo y el marcador de selección; c) crecimiento y selección de las células transformadas que contienen el gen heterólogo en un medio apropiado, caracterizado porque se efectúa una etapa de decoloración de las células vegetales competentes antes de la etapa de transformación (b), al introducir en el medio apropiado de cultivo de las células vegetales competentes una cantidad apropiada de inhibidor de HPPD. Se describen y reivindican además, los objetos restantes mencionados en el título.
ARP010103848A 2000-08-11 2001-08-10 Procedimiento para transformar celulas vegetales, por la introduccion en dichas celulas de un gen heterologo, con un gen de tolerancia a los inhibidores de hppd, como marcador de seleccion y procedimiento de obtencion de plantas transgenicas que comprenden dicho gen heterologo integrado en su genoma AR034140A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR0010601A FR2812883B1 (fr) 2000-08-11 2000-08-11 Utilisation d'inhibiteurs d'hppd comme agents de selection dans la transformation de plantes

Publications (1)

Publication Number Publication Date
AR034140A1 true AR034140A1 (es) 2004-02-04

Family

ID=8853515

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP010103848A AR034140A1 (es) 2000-08-11 2001-08-10 Procedimiento para transformar celulas vegetales, por la introduccion en dichas celulas de un gen heterologo, con un gen de tolerancia a los inhibidores de hppd, como marcador de seleccion y procedimiento de obtencion de plantas transgenicas que comprenden dicho gen heterologo integrado en su genoma

Country Status (7)

Country Link
US (1) US6791014B2 (es)
EP (1) EP1186666B1 (es)
AR (1) AR034140A1 (es)
AT (1) ATE306553T1 (es)
BR (1) BR0104564A (es)
DE (1) DE60113936T2 (es)
FR (1) FR2812883B1 (es)

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Also Published As

Publication number Publication date
ATE306553T1 (de) 2005-10-15
US20020100076A1 (en) 2002-07-25
EP1186666A3 (fr) 2002-04-17
DE60113936T2 (de) 2006-07-06
BR0104564A (pt) 2002-06-04
DE60113936D1 (de) 2006-02-23
EP1186666B1 (fr) 2005-10-12
US6791014B2 (en) 2004-09-14
EP1186666A2 (fr) 2002-03-13
FR2812883A1 (fr) 2002-02-15
FR2812883B1 (fr) 2002-10-18

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