AR034140A1 - Procedimiento para transformar celulas vegetales, por la introduccion en dichas celulas de un gen heterologo, con un gen de tolerancia a los inhibidores de hppd, como marcador de seleccion y procedimiento de obtencion de plantas transgenicas que comprenden dicho gen heterologo integrado en su genoma - Google Patents
Procedimiento para transformar celulas vegetales, por la introduccion en dichas celulas de un gen heterologo, con un gen de tolerancia a los inhibidores de hppd, como marcador de seleccion y procedimiento de obtencion de plantas transgenicas que comprenden dicho gen heterologo integrado en su genomaInfo
- Publication number
- AR034140A1 AR034140A1 ARP010103848A ARP010103848A AR034140A1 AR 034140 A1 AR034140 A1 AR 034140A1 AR P010103848 A ARP010103848 A AR P010103848A AR P010103848 A ARP010103848 A AR P010103848A AR 034140 A1 AR034140 A1 AR 034140A1
- Authority
- AR
- Argentina
- Prior art keywords
- gene
- cells
- procedure
- selection marker
- competent
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Cell Biology (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
Se describe y reivindica un procedimiento de transformación de células vegetales por introducción de un gen heterólogo en dichas células vegetales con un gen de tolerancia a los inhibidores de HPPD como marcador de selección, comprendiendo dicho procedimiento las etapas de: a) preparación y cultivo de células vegetales competentes destinadas a recibir el gen heterólogo en un medio apropiado; b) transformación de las células competentes con el gen heterólogo y el marcador de selección; c) crecimiento y selección de las células transformadas que contienen el gen heterólogo en un medio apropiado, caracterizado porque se efectúa una etapa de decoloración de las células vegetales competentes antes de la etapa de transformación (b), al introducir en el medio apropiado de cultivo de las células vegetales competentes una cantidad apropiada de inhibidor de HPPD. Se describen y reivindican además, los objetos restantes mencionados en el título.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
FR0010601A FR2812883B1 (fr) | 2000-08-11 | 2000-08-11 | Utilisation d'inhibiteurs d'hppd comme agents de selection dans la transformation de plantes |
Publications (1)
Publication Number | Publication Date |
---|---|
AR034140A1 true AR034140A1 (es) | 2004-02-04 |
Family
ID=8853515
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ARP010103848A AR034140A1 (es) | 2000-08-11 | 2001-08-10 | Procedimiento para transformar celulas vegetales, por la introduccion en dichas celulas de un gen heterologo, con un gen de tolerancia a los inhibidores de hppd, como marcador de seleccion y procedimiento de obtencion de plantas transgenicas que comprenden dicho gen heterologo integrado en su genoma |
Country Status (7)
Country | Link |
---|---|
US (1) | US6791014B2 (es) |
EP (1) | EP1186666B1 (es) |
AR (1) | AR034140A1 (es) |
AT (1) | ATE306553T1 (es) |
BR (1) | BR0104564A (es) |
DE (1) | DE60113936T2 (es) |
FR (1) | FR2812883B1 (es) |
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US7879540B1 (en) | 2000-08-24 | 2011-02-01 | Promega Corporation | Synthetic nucleic acid molecule compositions and methods of preparation |
US20030157643A1 (en) * | 2000-08-24 | 2003-08-21 | Almond Brian D | Synthetic nucleic acids from aquatic species |
US7728118B2 (en) * | 2004-09-17 | 2010-06-01 | Promega Corporation | Synthetic nucleic acid molecule compositions and methods of preparation |
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US8592650B2 (en) * | 2009-11-23 | 2013-11-26 | Bayer Cropscience N.V. | Elite event EE-GM3 and methods and kits for identifying such event in biological samples |
SI2503873T1 (sl) | 2009-11-23 | 2017-07-31 | Bayer Cropscience Nv | Na herbicide tolerantne rastline soje in postopki njihove prepoznave |
WO2011069893A1 (en) | 2009-12-08 | 2011-06-16 | Basf Se | Pesticidal mixtures |
EA023345B1 (ru) | 2009-12-08 | 2016-05-31 | Басф Се | Пестицидные смеси |
ES2668222T3 (es) | 2009-12-23 | 2018-05-17 | Bayer Intellectual Property Gmbh | Plantas tolerantes a herbicidas inhibidores de la HPPD |
EP2516630B1 (en) | 2009-12-23 | 2017-11-15 | Bayer Intellectual Property GmbH | Plants tolerant to hppd inhibitor herbicides |
UY33140A (es) | 2009-12-23 | 2011-07-29 | Bayer Cropscience Ag | Plantas tolerantes a herbicidas inhibidores de las hppd |
AU2010334818B2 (en) | 2009-12-23 | 2015-07-09 | Bayer Intellectual Property Gmbh | Plants tolerant to HPPD inhibitor herbicides |
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EP2531601B1 (en) * | 2010-02-02 | 2016-11-16 | Bayer Intellectual Property GmbH | Soybean transformation using HPPD inhibitors as selection agents |
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WO2011161131A1 (en) | 2010-06-25 | 2011-12-29 | Basf Se | Herbicidal mixtures |
WO2011161132A1 (en) | 2010-06-25 | 2011-12-29 | Basf Se | Pesticidal mixtures |
AU2011289283B2 (en) | 2010-08-13 | 2015-04-30 | Pioneer Hi-Bred International, Inc. | Chimeric promoters and methods of use |
WO2012022729A2 (en) | 2010-08-20 | 2012-02-23 | Basf Se | Method for improving the health of a plant |
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WO2012045737A1 (en) | 2010-10-07 | 2012-04-12 | Basf Se | Use of strobilurins for increasing the gluten strength in winter cereals |
US20130274104A1 (en) | 2010-12-22 | 2013-10-17 | Basf Se | Agrochemical mixtures for increasing the health of a plant |
US9078446B2 (en) | 2011-03-25 | 2015-07-14 | Bayer Intellectual Property Gmbh | Use of N-(tetrazol-4-yl)- or N-(triazol-3-yl)arylcarboxamides or their salts for controlling unwanted plants in areas of transgenic crop plants being tolerant to HPPD inhibitor herbicides |
WO2012130684A1 (en) | 2011-03-25 | 2012-10-04 | Bayer Cropscience Ag | Use of n-(1,2,5-oxadiazol-3-yl)benzamides for controlling unwanted plants in areas of transgenic crop plants being tolerant to hppd inhibitor herbicides |
EP2830420A1 (en) | 2012-03-29 | 2015-02-04 | Basf Se | Co-crystals of dicamba and a co-crystal former b |
JP6557142B2 (ja) | 2012-09-14 | 2019-08-07 | バイエル クロップサイエンス エルピーBayer Cropscience Lp | Hppd変異体および使用方法 |
MX2015004175A (es) | 2012-10-01 | 2015-06-10 | Basf Se | Uso de compuestos de n-tio-antranilamida en plantas cultivadas. |
WO2014079820A1 (en) | 2012-11-22 | 2014-05-30 | Basf Se | Use of anthranilamide compounds for reducing insect-vectored viral infections |
JP2016516011A (ja) | 2013-03-07 | 2016-06-02 | ビーエーエスエフ ソシエタス・ヨーロピアBasf Se | ピリメタニル及び選択されたジチインテトラカルボキシイミドの共結晶 |
RU2020105862A (ru) | 2013-03-07 | 2020-02-28 | Атеникс Корп. | Гены токсинов и способы их применения |
US10876130B2 (en) | 2014-03-11 | 2020-12-29 | BASF Agricultural Solutions Seed US LLC | HPPD variants and methods of use |
EP2979549A1 (en) | 2014-07-31 | 2016-02-03 | Basf Se | Method for improving the health of a plant |
EP3028573A1 (en) | 2014-12-05 | 2016-06-08 | Basf Se | Use of a triazole fungicide on transgenic plants |
WO2016091674A1 (en) | 2014-12-12 | 2016-06-16 | Basf Se | Use of cyclaniliprole on cultivated plants |
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EP3054014A3 (en) | 2016-05-10 | 2016-11-23 | BASF Plant Science Company GmbH | Use of a fungicide on transgenic plants |
WO2018098214A1 (en) | 2016-11-23 | 2018-05-31 | Bayer Cropscience Lp | Axmi669 and axmi991 toxin genes and methods for their use |
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WO2018165091A1 (en) | 2017-03-07 | 2018-09-13 | Bayer Cropscience Lp | Hppd variants and methods of use |
CA3055389A1 (en) | 2017-03-07 | 2018-09-13 | BASF Agricultural Solutions Seed US LLC | Hppd variants and methods of use |
US11279944B2 (en) | 2017-10-24 | 2022-03-22 | BASF Agricultural Solutions Seed US LLC | Of herbicide tolerance to 4-hydroxyphenylpyruvate dioxygenase (HPPD) inhibitors by down-regulation of HPPD expression in soybean |
WO2019083808A1 (en) | 2017-10-24 | 2019-05-02 | Basf Se | IMPROVING HERBICIDE TOLERANCE AGAINST HPPD INHIBITORS BY REGULATION OF PUTATIVE REDUCED 4-HYDROXYPHENYLPYRUVATE REDUCES IN SOYBEANS |
WO2019233349A1 (zh) | 2018-06-04 | 2019-12-12 | 青岛清原化合物有限公司 | 突变型对羟苯基丙酮酸双氧化酶、其编码核酸以及应用 |
CN110771443B (zh) * | 2019-11-22 | 2021-12-21 | 阳山县三连阳生态农林开发有限公司 | 阳山白及单一培养基配方无菌播种成苗生产工艺流程 |
WO2023044364A1 (en) | 2021-09-15 | 2023-03-23 | Enko Chem, Inc. | Protoporphyrinogen oxidase inhibitors |
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WO2024137438A2 (en) | 2022-12-19 | 2024-06-27 | BASF Agricultural Solutions Seed US LLC | Insect toxin genes and methods for their use |
Family Cites Families (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4443971A (en) * | 1979-10-16 | 1984-04-24 | Cornell Research Foundation, Inc. | Herbicide-tolerant plants |
US5217902A (en) * | 1989-05-26 | 1993-06-08 | Dna Plant Technology Corporation | Method of introducing spectinomycin resistance into plants |
US5073675A (en) | 1989-05-26 | 1991-12-17 | Dna Plant Technology Corporation | Method of introducing spectinomycin resistance into plants |
FR2734842B1 (fr) * | 1995-06-02 | 1998-02-27 | Rhone Poulenc Agrochimie | Sequence adn d'un gene de l'hydroxy-phenyl pyruvate dioxygenase et obtention de plantes contenant un gene de l'hydroxy-phenyl pyruvate dioxygenase, tolerantes a certains herbicides |
US6245968B1 (en) * | 1997-11-07 | 2001-06-12 | Aventis Cropscience S.A. | Mutated hydroxyphenylpyruvate dioxygenase, DNA sequence and isolation of plants which contain such a gene and which are tolerant to herbicides |
FR2770854B1 (fr) * | 1997-11-07 | 2001-11-30 | Rhone Poulenc Agrochimie | Sequence adn d'un gene de l'hydroxy-phenyl pyruvate dioxygenase et obtention de plantes contenant un tel gene, tolerantes aux herbicides |
-
2000
- 2000-08-11 FR FR0010601A patent/FR2812883B1/fr not_active Expired - Fee Related
-
2001
- 2001-07-19 AT AT01420162T patent/ATE306553T1/de not_active IP Right Cessation
- 2001-07-19 DE DE60113936T patent/DE60113936T2/de not_active Expired - Lifetime
- 2001-07-19 EP EP01420162A patent/EP1186666B1/fr not_active Expired - Lifetime
- 2001-08-02 US US09/921,263 patent/US6791014B2/en not_active Expired - Lifetime
- 2001-08-09 BR BR0104564-4A patent/BR0104564A/pt not_active IP Right Cessation
- 2001-08-10 AR ARP010103848A patent/AR034140A1/es unknown
Also Published As
Publication number | Publication date |
---|---|
ATE306553T1 (de) | 2005-10-15 |
US20020100076A1 (en) | 2002-07-25 |
EP1186666A3 (fr) | 2002-04-17 |
DE60113936T2 (de) | 2006-07-06 |
BR0104564A (pt) | 2002-06-04 |
DE60113936D1 (de) | 2006-02-23 |
EP1186666B1 (fr) | 2005-10-12 |
US6791014B2 (en) | 2004-09-14 |
EP1186666A2 (fr) | 2002-03-13 |
FR2812883A1 (fr) | 2002-02-15 |
FR2812883B1 (fr) | 2002-10-18 |
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